Journal of Biomedical Research最新文献_第5页

Atherosclerosis originating from childhood: Specific features. 源自童年的动脉粥样硬化：具体特征。

IF 2.3 4区医学

Journal of Biomedical Research Pub Date : 2024-05-22 DOI: 10.7555/JBR.37.20230198

Anastasia V Poznyak, Alexey A Yakovlev, Mikhail А Popov, Elena B Zhigmitova, Vasily N Sukhorukov, Alexander N Orekhov

引用次数: 0

hUCMSC-derived extracellular vesicles relieve cisplatin-induced granulosa cell apoptosis in mice by transferring anti-apoptotic miRNAs. 源自 hUCMSC 的细胞外囊泡通过转移抗凋亡 miRNA 缓解顺铂诱导的小鼠颗粒细胞凋亡。

IF 2.3 4区医学

Journal of Biomedical Research Pub Date : 2024-04-25 DOI: 10.7555/JBR.37.20230310

Wenjing Tang, Haiyan Yan, Xiaojun Chen, Yanan Pu, Xin Qi, Liyang Dong, Chuan Su

{"title":"hUCMSC-derived extracellular vesicles relieve cisplatin-induced granulosa cell apoptosis in mice by transferring anti-apoptotic miRNAs.","authors":"Wenjing Tang, Haiyan Yan, Xiaojun Chen, Yanan Pu, Xin Qi, Liyang Dong, Chuan Su","doi":"10.7555/JBR.37.20230310","DOIUrl":"https://doi.org/10.7555/JBR.37.20230310","url":null,"abstract":"Premature ovarian insufficiency (POI) caused by chemotherapy is a common complication in female cancer survivors of childbearing age. Traditional methods including mesenchymal stem cell (MSC) transplant and hormone replacement therapy have limited clinical application due to their drawbacks, and more methods need to be developed. In the current study, the potential effects and underlying mechanisms of human umbilical cord MSC-derived extracellular vesicles (hUCMSC-EVs) were investigated in a cisplatin (CDDP)-induced POI mouse model and a human granulosa cell (GC) line. The results showed that hUCMSC-EVs significantly attenuated body weight loss, ovarian weight loss, ovary atrophy, and follicle loss in moderate-dose (1.5 mg/kg) CDDP-induced POI mice, similar to the effects observed with hUCMSCs. We further discovered that the hUCMSC-EVs might inhibit CDDP-induced ovarian GC apoptosis by upregulating anti-apoptotic miRNA levels in GCs, thereby downregulating the mRNA levels of multiple pro-apoptotic genes. In general, our findings indicate that moderate-dose chemotherapy may be a better choice for clinical oncotherapy considering the effective rescue of oncotherapy-induced ovarian damage with hUCMSC-EVs. Additionally, multiple miRNAs in hUCMSC-EVs may potentially be used to inhibit chemotherapy-induced ovarian GC apoptosis, thereby restoring ovarian function and improving the life quality of female cancer patients.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-15"},"PeriodicalIF":2.3,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA-sequencing expression profile and functional analysis of retinal pigment epithelium in atrophic age-related macular degeneration. 萎缩性老年性黄斑变性患者视网膜色素上皮细胞的 RNA 序列表达谱和功能分析。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-04-25 DOI: 10.7555/JBR.37.20230320

Miao Xu, Yan Gao, Wenjie Yin, Qinghuai Liu, Songtao Yuan

{"title":"RNA-sequencing expression profile and functional analysis of retinal pigment epithelium in atrophic age-related macular degeneration.","authors":"Miao Xu, Yan Gao, Wenjie Yin, Qinghuai Liu, Songtao Yuan","doi":"10.7555/JBR.37.20230320","DOIUrl":"10.7555/JBR.37.20230320","url":null,"abstract":"The retinal pigment epithelium (RPE) is fundamental to sustaining retinal homeostasis. RPE abnormality leads to visual defects and blindness, including age-related macular degeneration (AMD). Although breakthroughs have been made in the treatment of neovascular AMD, effective intervention for atrophic AMD is largely absent. The inadequate knowledge of RPE pathology is hindered by a lack of patient RPE datasets, especially at the single-cell resolution. In this study, we delved into a large-scale single-cell resource of AMD donors in which RPE cells were occupied in a substantial proportion. Bulk RNA-seq datasets of atrophic AMD were integrated to extract molecular characteristics of RPE in the pathogenesis of atrophic AMD. Both in vivo and in vitro models revealed that carboxypeptidase X, M14 family member 2 (CPXM2) was specifically expressed in the RPE cells of atrophic AMD, which might be induced by oxidative stress and involved in the epithelial-mesenchymal transition of RPE cells. Additionally, silencing of CPXM2 inhibited the mesenchymal phenotype of RPE cells in an oxidative stress cell model. Thus, our results demonstrate that CPXM2 plays a crucial role in regulating atrophic AMD and may serve as a potential therapeutic target for atrophic AMD.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-12"},"PeriodicalIF":2.2,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First-episode psychiatric disorder risk from SARS-CoV-2 infection, a clinical analysis with Chinese psychiatric inpatients. 中国精神病住院患者感染 SARS-CoV-2 导致首发精神障碍的风险临床分析。

IF 2.3 4区医学

Journal of Biomedical Research Pub Date : 2024-04-25 DOI: 10.7555/JBR.38.20240005

Ya Xie, Zifeng Xu, YuMin Zhang, Yisheng Li, Pengyu Du, Chun Wang

{"title":"First-episode psychiatric disorder risk from SARS-CoV-2 infection, a clinical analysis with Chinese psychiatric inpatients.","authors":"Ya Xie, Zifeng Xu, YuMin Zhang, Yisheng Li, Pengyu Du, Chun Wang","doi":"10.7555/JBR.38.20240005","DOIUrl":"https://doi.org/10.7555/JBR.38.20240005","url":null,"abstract":"The extensive spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) throughout China in late 2022 has underscored the correlation between this virus and severe psychiatric disorders. Nevertheless, there remains a dearth of reported corresponding clinical and pathological features. Accordingly, we retrospectively reviewed the electronic medical records of psychiatric inpatients for seven days from early January 2023. Twenty-one inpatients who developed first-episode psychiatric disorders within two weeks after SARS-CoV-2 infection were recruited, while 24 uninfected the first-episode psychiatric inpatients were selected as controls. Comparative analyses of clinical manifestations, routine laboratory, and imaging examinations were performed. Our investigation revealed a 330% increase in first-episode psychiatric inpatients incidence after SARS-CoV-2 infection in 2023 compared to the preceding year without infections. Most cases exhibited psychiatric symptoms within a week of infection, resolving about two weeks with no residual symptoms after a three month. One-way ANOVA analysis between inpatients characterized by psychotic symptoms and hyperthermia was significant. Infected inpatients displayed elevated cytokine levels of interleukin-4, interleukin-8, and interferon-α, and decreased levels of eosinophils and basophils. These finding suggested that SARS-CoV-2 may contribute to the development of psychiatric disorders, likely mediated by the virus-induced inflammatory response and neuronal dysfunction in the context of psychological distress.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-11"},"PeriodicalIF":2.3,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of pericyte in ocular vascular diseases. 眼周膜在眼部血管疾病中的作用。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-04-25 DOI: 10.7555/JBR.37.20230314

Lian Jun Shi, HuiMin Ge, Fan Ye, Xiumiao Li, Qin Jiang

{"title":"The role of pericyte in ocular vascular diseases.","authors":"Lian Jun Shi, HuiMin Ge, Fan Ye, Xiumiao Li, Qin Jiang","doi":"10.7555/JBR.37.20230314","DOIUrl":"10.7555/JBR.37.20230314","url":null,"abstract":"Pericytes are located in the stromal membrane of the capillary outer wall and contain endothelial cells (ECs). They are pivotal in regulating blood flow, enhancing vascular stability, and maintaining the integrity of the blood-retina barrier (BRB)/blood-brain barrier (BBB). The pluripotency of pericytes allows them to differentiate into various cell types, highlighting their significance in vascular disease pathogenesis, as demonstrated by previous studies. This potential enables pericytes to be a potential biomarker for the diagnosis and a target for treatment of vascular disorders. The retina, an essential part of the eyeball, is an extension of cerebral tissue with a transparent refractive medium. It offers a unique window for assessing systemic microvascular lesions. Routine fundus examination is necessary for patients with diabetes and hypertension. Manifestations, such as retinal artery tortuosity, dilation, stenosis, and abnormal arteriovenous anastomosis, serve as typical hallmarks of retinal vasculopathy. Therefore, studies of ocular vascular diseases significantly facilitate the exploration of systemic vascular diseases.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-10"},"PeriodicalIF":2.2,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11629158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mouse KL2 is a unique MTSE involved in chromosome-based spindle organization and regulated by multiple kinases during female meiosis. 小鼠 KL2 是一种独特的 MTSE，参与基于染色体的纺锤体组织，在雌性减数分裂过程中受多种激酶调控。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-04-25 DOI: 10.7555/JBR.37.20230290

Shi-Ya Xie, Yan-Jie Yang, Zhen Jin, Xiao-Cong Liu, Shu-Ping Zhang, Ning Su, Jia-Qi Liu, Cong-Rong Li, Dong Zhang, Lei-Lei Gao, Zhi-Xia Yang

{"title":"Mouse KL2 is a unique MTSE involved in chromosome-based spindle organization and regulated by multiple kinases during female meiosis.","authors":"Shi-Ya Xie, Yan-Jie Yang, Zhen Jin, Xiao-Cong Liu, Shu-Ping Zhang, Ning Su, Jia-Qi Liu, Cong-Rong Li, Dong Zhang, Lei-Lei Gao, Zhi-Xia Yang","doi":"10.7555/JBR.37.20230290","DOIUrl":"10.7555/JBR.37.20230290","url":null,"abstract":"Microtubule-severing enzymes (MTSEs) play important roles in mitosis and meiosis of the primitive organisms. However, no studies have assessed their roles in mammalian meiosis of females, whose abnormality accounts for over 80% of the cases of gamete-originated human reproductive disease. In the current study, we reported that katanin-like 2 (KL2) was the only MTSE concentrating at chromosomes. Furthermore, the knockdown of KL2 significantly reduced chromosome-based increase in the microtubule (MT) polymer, increased aberrant kinetochore-MT (K-MT) attachment, delayed meiosis, and severely affected normal fertility. Importantly, we demonstrated that the inhibition of aurora B, a key kinase for correcting aberrant K-MT attachment, eliminated KL2 from chromosomes completely. KL2 also interacted with phosphorylated eukaryotic elongation factor-2 kinase; they competed for chromosome binding. We also observed that the phosphorylated KL2 was localized at spindle poles, and that KL2 phosphorylation was regulated by extracellular signal-regulated kinase 1/2. In summary, our study reveals a novel function of MTSEs in mammalian female meiosis and demonstrates that multiple kinases coordinate to regulate the levels of KL2 at chromosomes.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-15"},"PeriodicalIF":2.2,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coronary atherosclerotic plaque regression strategies. 冠状动脉粥样硬化斑块消退策略。

IF 2.3 4区医学

Journal of Biomedical Research Pub Date : 2024-04-25 DOI: 10.7555/JBR.37.20230223

Anastasia V Poznyak, Alexey Aleksandrovich Yakovlev, Mikhail А Popov, Alexander D Zhuravlev, Vasily N Sukhorukov, Alexander N Orekhov

引用次数: 0

Ferroptosis: Iron-mediated cell death linked to disease pathogenesis. 铁变态反应：铁介导的细胞死亡与疾病发病机制有关。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-03-25 DOI: 10.7555/JBR.37.20230224

Xiangyu Zhang, Yingchao Hu, Bingwei Wang, Shuo Yang

引用次数: 0

Evaluating short-term and long-term liver fibrosis improvement in hepatitis C patients post-DAA treatment. 评估 DAA 治疗后丙型肝炎患者肝纤维化的短期和长期改善情况。

IF 2.2 4区医学

Journal of Biomedical Research Pub Date : 2024-03-21 DOI: 10.7555/JBR.37.20230284

Yifan Wang, Xinyan Ma, Yanzheng Zou, Ming Yue, Meiling Zhang, Rongbin Yu, Hongbo Chen, Peng Huang

{"title":"Evaluating short-term and long-term liver fibrosis improvement in hepatitis C patients post-DAA treatment.","authors":"Yifan Wang, Xinyan Ma, Yanzheng Zou, Ming Yue, Meiling Zhang, Rongbin Yu, Hongbo Chen, Peng Huang","doi":"10.7555/JBR.37.20230284","DOIUrl":"10.7555/JBR.37.20230284","url":null,"abstract":"Despite achieving a high cure rate with direct-acting antivirals (DAAs) in hepatitis C treatment, further research is needed to identify additional benefits of the DAA therapy. The current study evaluated liver fibrosis improvement in 848 hepatitis C patients treated with DAAs, who also achieved sustained virologic response (SVR). Using the fibrosis-4 (FIB-4) index, patients were categorized based on their baseline fibrosis level, and improvements in fibrosis were analyzed in both the short-term (9-26 weeks) and long-term (≥ 36 weeks) follow-up. The results showed a significant decrease in the FIB-4 index, indicating an improvement in liver fibrosis, in 63.00% of the patients during the short-term follow-up and 67.56% during the long-term follow-up. Short-term improvement was associated with factors including ribavirin (RBV) usage, blood cholinesterase levels, alanine transaminase levels, albumin levels, and the baseline FIB-4 index. Additionally, long-term improvement was associated with factors such as aspartate transaminase levels, total protein level, and the baseline FIB-4 index. The current study emphasizes the importance of continuous assessment and post-treatment monitoring of liver fibrosis, providing crucial insights for enhancing patient care in hepatitis C management.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"1-10"},"PeriodicalIF":2.2,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional aspects of the brain lymphatic drainage system in aging and neurodegenerative diseases. 脑淋巴引流系统在衰老和神经退行性疾病中的功能。

IF 2.3 4区医学

Journal of Biomedical Research Pub Date : 2024-03-02 DOI: 10.7555/JBR.37.20230264

Yan Chen, Xiaoxin He, Jiachen Cai, Qian Li

{"title":"Functional aspects of the brain lymphatic drainage system in aging and neurodegenerative diseases.","authors":"Yan Chen, Xiaoxin He, Jiachen Cai, Qian Li","doi":"10.7555/JBR.37.20230264","DOIUrl":"10.7555/JBR.37.20230264","url":null,"abstract":"The phenomenon of an aging population is advancing at a precipitous rate. Alzheimer's disease (AD) and Parkinson's disease (PD) are two of the most common age-associated neurodegenerative diseases, both of which are primarily characterized by the accumulation of toxic proteins and the progressive demise of neuronal structures. Recent discoveries about the brain lymphatic drainage system have precipitated a growing body of investigations substantiating its novel roles, including the clearance of macromolecular waste and the trafficking of immune cells. Notably, aquaporin 4-mediated glymphatic transport, crucial for maintaining neural homeostasis, becomes disrupted during the aging process and is further compromised in the pathogenesis of AD and PD. Functional meningeal lymphatic vessels, which facilitate the drainage of cerebrospinal fluid into the deep cervical lymph nodes, are integral in bridging the central nervous system with peripheral immune responses. Dysfunction in these meningeal lymphatic vessels exacerbates pathological trajectory of the age-related neurodegenerative disease. This review explores modulatory influence of the glymphatic system and meningeal lymphatic vessels on the aging brain and its associated neurodegenerative disorders. It also encapsulates the insights of potential mechanisms and prospects of the targeted non-pharmacological interventions.","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"206-221"},"PeriodicalIF":2.3,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11144931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140012658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0