Journal of Biomedical Research最新文献

{"title":"Author Correction: A novel recombinant DNA vaccine encoding <i>Mycobacterium tuberculosis</i> ESAT-6 and FL protects against <i>Mycobacterium tuberculosis</i> challenge in mice.","authors":"Qingtao Jiang,&nbsp;Jing Zhang,&nbsp;Xia Chen,&nbsp;Mei Xia,&nbsp;Yanlai Lu,&nbsp;Wen Qiu,&nbsp;Ganzhu Feng,&nbsp;Dan Zhao,&nbsp;Yan Li,&nbsp;Fengxia He,&nbsp;Guangyong Peng,&nbsp;Yingwei Wang","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":"36 6","pages":"448-450"},"PeriodicalIF":2.3,"publicationDate":"2022-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10541036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between soft drink consumption types and risk of lung cancer and all-cancer: A prospective study of PLCO data.","authors":"Dongfang You,&nbsp;Hongyang Xu,&nbsp;Xin Chen,&nbsp;Jiawei Zhou,&nbsp;Yaqian Wu,&nbsp;Yingdan Tang,&nbsp;Zhongtian Wang,&nbsp;Yang Zhao,&nbsp;Fang Shao","doi":"10.7555/JBR.36.20220135","DOIUrl":"https://doi.org/10.7555/JBR.36.20220135","url":null,"abstract":"<p><p>Diet/sugar-free soft drinks are considered to be healthier than regular soft drinks. However, few studies have examined the relationship between the types of soft drinks (regular and diet/sugar-free) and lung cancer (LC)/all-cancer (AC) risk. In this study, we comprehensively assessed the influence of the type of soft drink consumption on LC/AC risk based on the Prostate, Lung, Colorectal, and Ovarian (PLCO) Cancer Screening Trial. Multivariable Cox proportional hazards and competing risks Fine-Gray regression models adjusted for relevant confounders were used to estimate hazard ratios (HRs) and subdistribution HRs for different types of soft drink consumption. In the PLCO population, female subgroup, and the ever/current smoker subgroup, consumption of both regular and diet soft drinks was associated with a significantly reduced risk of LC compared with no soft drinks at all. For the non-lung cancer (NLC) risk, consumption of only diet soft drinks had a significant positive association for the total population and female subgroup. Based on our findings, it was suggested that partial replacement of regular soft drinks with diet soft drinks might be beneficial to LC prevention, especially for females and ever/current smokers. Additionally, completely replacing regular soft drinks with diet soft drinks might be detrimental to NLC prevention, especially for females.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":"36 6","pages":"390-400"},"PeriodicalIF":2.3,"publicationDate":"2022-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10534512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"hucMSC-derived exosomes protect ovarian reserve and restore ovarian function in cisplatin treated mice.","authors":"Yue Xiao,&nbsp;Yue Peng,&nbsp;Chi Zhang,&nbsp;Wei Liu,&nbsp;Kehan Wang,&nbsp;Jing Li","doi":"10.7555/JBR.36.20220166","DOIUrl":"10.7555/JBR.36.20220166","url":null,"abstract":"<p><p>Anti-cancer therapy often causes premature ovarian insufficiency and infertility as the ovarian follicle reserve is extremely sensitive to chemotherapy drugs, such as cisplatin. Various fertility preservation methods have been explored for women, especially prepubertal girls undergoing radiotherapy and chemotherapy due to cancer. In recent years, mesenchymal stem cell-derived exosomes (MSC-exos) have been reported to play an important role in tissue repair and the treatment of various diseases. In the current study, we observed that human umbilical cord-derived MSC-exos (hucMSC-exos) after short-term culture improved follicular survival and development while receiving cisplatin treatment. Moreover, intravenous injection of hucMSC-exos improved ovarian function and ameliorated inflammatory environment within the ovary. The underlying mechanism of hucMSC-exos on fertility preservation was associated with the down-regulation of p53-related apoptosis and their anti-inflammatory function. Based on these findings, we propose that hucMSC-exos may be a potential approach to improve fertility in women diagnosed with cancer.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":" ","pages":"382-393"},"PeriodicalIF":2.3,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9481414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A pilot study of the relative number of circulating tumor cells and leukocytes containing actin-binding proteins in head and neck cancer patients.","authors":"Gelena Kakurina,&nbsp;Marina Stakheeva,&nbsp;Elena Sereda,&nbsp;Evgenia Sidenko,&nbsp;Olga Cheremisina,&nbsp;Evgeny Choinzonov,&nbsp;Irina Kondakova","doi":"10.7555/JBR.36.20220182","DOIUrl":"https://doi.org/10.7555/JBR.36.20220182","url":null,"abstract":"<p><p>Circulating tumor cells (CTCs) play an important role in tumor metastases, which is positively correlated with an increased risk of death. Actin-binding proteins, including cofilin (CFL1), profilin 1 (PFN1), and adenylate cyclase-associated protein 1 (CAP1), are thought to be involved in tumor cell motility and metastasis, specifically in head and neck squamous cell carcinoma (HNSCC). However, currently, there are no published studies on CFL1, PFN1, and CAP1 in CTCs and leukocytes in HNSCC patients. We assessed serum levels of CFL1, PFN1, and CAP1 and the number of CTCs and leukocytes containing these proteins in blood from 31 HNSCC patients (T1-4N0-2M0). The analysis used flow cytometry and an enzyme-linked immunosorbent assay kit. We found that CAP1 ⁺ CTCs and CAP1 ⁺ leukocyte subpopulations were prevalent in these HNSCC patient samples, while the prevalence rates of CFL1 ⁺ and PFN1 ⁺ CTCs were relatively low. Patients with stage T2-4N1-2M0 had CFL1 ⁺ and PFN1 ⁺ CTCs with an elevated PFN1 serum level, compared with the T1-3N0M0 group. In summary, the PFN1 serum level and the relative number of PFN1 ⁺CD326 ⁺ CTCs could be valuable prognostic markers for HNSCC metastases. The current study is the first to obtain data regarding the contents of actin-binding proteins (ABPs) in CTCs, and leukocytes in blood from HNSCC patients. This is also the first to assess the relationship between the number of CTCs subgroups and disease characteristics.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":"37 3","pages":"213-224"},"PeriodicalIF":2.3,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10226087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9545426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value of AKT isoforms in non-small cell lung adenocarcinoma.","authors":"Sahil Khurana,&nbsp;Ajay Pal Singh,&nbsp;Ashok Kumar,&nbsp;Rajeev Nema","doi":"10.7555/JBR.36.20220138","DOIUrl":"https://doi.org/10.7555/JBR.36.20220138","url":null,"abstract":"Dear Editor, Lung cancer is one of the most prevalent cancers in the world and has a high mortality rate. Lung cancer patients often have a poor prognosis, with a five-year survival rate of only about 16%. The International Agency for Research on Cancer reports that lung cancer was the main cause of cancer deaths in 2020, accounting for 1.80 million deaths. Due to the dismal overall prognosis of lung cancer, there is an urgent need to develop accurate and effective diagnostic tests that target specifically early oncogenic pathways in lung cancer patients to improve their prognosis. The two principal types of lung cancer are small cell lung carcinoma (SCLC) and non-small cell lung carcinoma (NSCLC), with NSCLC accounting for around 85% of all lung malignancies[1]. The region frequency and prevalence of the lung disease are controlled by both genotypic and phenotypic exposures. An accurate lung cancer diagnosis is essential for the patient's better survival for two main reasons: appropriate drug selection and effective treatment prediction. Histopathological diagnosis depends on cell shape and the nucleus-to-cytoplasm size ratio to distinguish SCLC from NSCLC. Surgical resection, aggressive or palliative radiation, and neoadjuvant chemotherapy are frequently used to treat lung cancer. In the modern era, gene-targeted treatments against tyrosine kinase inhibitors and antibodies against mutations in driver genes for lung cancer are being developed. Several mutations have been shown to be the most common in lung cancer. For example, mutations in the K-ras proto-oncogene cause 10% to 30% of lung adenocarcinoma (LUAD), while epidermal growth factor receptor mutations are more frequent in squamous cell lung cancer (SqCLC)[2]. Kaplan-Meier plotter (KM plotter) database (http://kmplot.com/analysis/) is a commonly used database for the real-time meta-analysis of published lung cancer microarray datasets to find survival biomarkers[3]. In a number of malignancies, the KM plotter has also been used to discover genes that may serve as possible prognostic indicators for postprogression survival (PPS), progression-free survival (PFS), and overall survival (OS)[3]. Many human malignancies, including lung cancer, have activated and overexpressed AKT isoforms[4]. AKT2 inhibition aids in the suppression of LUAD cell proliferation and colony expansion. Therefore, the significance of AKT isoforms in the diagnosis and prognosis of lung cancer was investigated in the current study. The association between gene specific mRNA expression and OS was analyzed by the KM plotter. Currently, gene expression and survival data from 1927 patients with a follow-up period of 20 years are available. Gene names of AKT isoforms (i.e., AKT1, AKT2, and AKT3) were entered into the KM plotter database to obtain survival plots. The association between mRNA expression levels of different AKT isoforms and the established clinicopathological features was studied. The patient data were linked t","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":"37 3","pages":"225-228"},"PeriodicalIF":2.3,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10226088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9543966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of an adaptation to hypoxia on cardiac tolerance to ischemia/reperfusion.","authors":"Natalia V Naryzhnaya, Leonid N Maslov, Ivan A Derkachev, Huijie Ma, Yi Zhang, N Rajendra Prasad, Nirmal Singh, Feng Fu, Jianming Pei, Akpay Sarybaev, Akylbek Sydykov","doi":"10.7555/JBR.36.20220125","DOIUrl":"10.7555/JBR.36.20220125","url":null,"abstract":"<p><p>The acute myocardial infarction (AMI) and sudden cardiac death (SCD), both associated with acute cardiac ischemia, are one of the leading causes of adult death in economically developed countries. The development of new approaches for the treatment and prevention of AMI and SCD remains the highest priority for medicine. A study on the cardiovascular effects of chronic hypoxia (CH) may contribute to the development of these methods. Chronic hypoxia exerts both positive and adverse effects. The positive effects are the infarct-reducing, vasoprotective, and antiarrhythmic effects, which can lead to the improvement of cardiac contractility in reperfusion. The adverse effects are pulmonary hypertension and right ventricular hypertrophy. This review presents a comprehensive overview of how CH enhances cardiac tolerance to ischemia/reperfusion. It is an in-depth analysis of the published data on the underlying mechanisms, which can lead to future development of the cardioprotective effect of CH. A better understanding of the CH-activated protective signaling pathways may contribute to new therapeutic approaches in an increase of cardiac tolerance to ischemia/reperfusion.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":"37 4","pages":"230-254"},"PeriodicalIF":2.2,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10387748/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9907734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glioblastoma multiforme: Diagnosis, treatment, and invasion.","authors":"Jiawei Li,&nbsp;Lili Feng,&nbsp;Yingmei Lu","doi":"10.7555/JBR.36.20220156","DOIUrl":"https://doi.org/10.7555/JBR.36.20220156","url":null,"abstract":"<p><p>Glioblastoma multiforme (GBM) is an essentially incurable brain tumor, which has been explored for approximately a century. Nowadays, surgical resection, chemotherapy, and radiation therapy are still the standardized therapeutic options. However, due to the intrinsic invasion and metastasis features and the resistance to chemotherapy, the survival rate of glioblastoma patients remains unsatisfactory. To improve the current situation, much more research is needed to provide comprehensive knowledge of GBM. In this review, we summarize the latest updates on GBM treatment and invasion. Firstly, we review the traditional and emerging therapies that have been used for GBM treatment. Given the limited efficiency of these therapies, we further discuss the role of invasion in GBM recurrence and progression, and present current research progress on the mode and mechanisms of GBM invasion.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":"37 1","pages":"47-58"},"PeriodicalIF":2.3,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898042/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10739917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction and evaluation of an alcohol vapor chamber system.","authors":"Wan Jiang,&nbsp;Jiajia Chen,&nbsp;Olivia Ewi Vidjro,&nbsp;Yingying Zhang,&nbsp;Gengni Guo,&nbsp;Ziyi Li,&nbsp;Yize Qi,&nbsp;Rouli Dai,&nbsp;Tengfei Ma","doi":"10.7555/JBR.36.20220151","DOIUrl":"https://doi.org/10.7555/JBR.36.20220151","url":null,"abstract":"<p><p>An increasing number of studies demonstrated that alcohol vapor chamber is an effective way to model physical signs of alcohol use disorders. Although researchers are developing different vapor chambers to study chronic alcohol exposure model worldwide, few studies build and modify their own vapor chambers in China. Here, we designed and established an alcohol vapor chamber system for small animals. We described a paradigm showing how to control and monitor alcohol concentration in whole system. The vapor chamber system with several advantages including accommodating up to ten standard mouse cages. Furthermore, the system was tested by evaluating the blood alcohol concentration and neuron injury in mice. Importantly, the alcohol withdrawal after vapor exposure caused motor coordination impairment, anxiolytic- and depression-like behavior. Finally, the N-methyl-D-aspartate receptor (NMDAR)-mediated glutamatergic transmissions in the medial prefrontal cortex was changed after alcohol vapor exposure-induced behaviors. The frequency and amplitude of spontaneous excitatory postsynaptic currents between control and alcohol groups were not different, suggesting that alcohol exposure-induced behaviors are associated with the change in NMDAR response. Taken together, the new alcohol vapor chamber system was constructed, which would help to research the relationship between the stable alcohol exposure and withdrawal behaviors and to study chronic alcohol exposure-induced disorders in China.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":"37 2","pages":"115-124"},"PeriodicalIF":2.3,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10018410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9565644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological functions and potential implications of circular RNAs.","authors":"Lan Ma,&nbsp;Haiyan Chu,&nbsp;Meilin Wang,&nbsp;Zhengdong Zhang","doi":"10.7555/JBR.36.20220095","DOIUrl":"https://doi.org/10.7555/JBR.36.20220095","url":null,"abstract":"<p><p>Circular RNAs (circRNAs) are characterized by a covalent closed-loop structure with an absence of both 5' cap structure and 3' polyadenylated tail. Numerous studies have found that circRNAs play an important role in various diseases and have a variety of biological regulatory mechanisms, including acting as microRNA sponges, interacting with proteins, modulating the expression of related genes and translating into peptides or proteins. CircRNAs have also been used as biomarkers for a number of diseases, which could improve clinical practice. This review summarizes the most recent advances in biogenesis and knowledge of the biological functions of circRNAs as well as the related bioinformatics databases. We specifically describe developments in understanding of circRNA functions in the field of environmental exposure-induced diseases. Finally, we focus on potential clinical implications of circRNAs to facilitate their clinical transformation into disease treatment.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":"37 2","pages":"89-99"},"PeriodicalIF":2.3,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10018409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9884290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impaired autophagy in amyloid-beta pathology: A traditional review of recent Alzheimer's research.","authors":"Minghao Yuan,&nbsp;Yangyang Wang,&nbsp;Zhenting Huang,&nbsp;Feng Jing,&nbsp;Peifeng Qiao,&nbsp;Qian Zou,&nbsp;Jing Li,&nbsp;Zhiyou Cai","doi":"10.7555/JBR.36.20220145","DOIUrl":"https://doi.org/10.7555/JBR.36.20220145","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is the most prevalent neurodegenerative disorder. The major pathological changes in AD progression are the generation and accumulation of amyloid-beta (Aβ) peptides as well as the presence of abnormally hyperphosphorylated tau proteins in the brain. Autophagy is a conserved degradation pathway that eliminates abnormal protein aggregates and damaged organelles. Previous studies have suggested that autophagy plays a key role in the production and clearance of Aβ peptides to maintain a steady-state of Aβ peptides levels. However, a growing body of evidence suggests that autophagy is significantly impaired in the pathogenesis of AD, especially in Aβ metabolism. Therefore, this article reviews the latest studies concerning the mechanisms of autophagy, the metabolism of Aβ peptides, and the defective autophagy in the production and clearance of Aβ peptides. Here, we also summarize the established and new strategies for targeting autophagy <i>in vivo</i> and through clinical AD trials to identify gaps in our knowledge and to generate further questions.</p>","PeriodicalId":15061,"journal":{"name":"Journal of Biomedical Research","volume":"37 1","pages":"30-46"},"PeriodicalIF":2.3,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9898044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9251319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}