作为精子质量和男性不育症潜在生物标志物的人类 circBOULE RNAs。

IF 2.2 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Liping Cheng, He Jin, Tianheng Xiao, Xiaoyu Yang, Tingting Zhao, Eugene Yujun Xu
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引用次数: 0

摘要

预测生育能力的可靠分子生物标志物仍然稀缺。本研究探索了睾丸特异性环BOULE RNA作为男性不育症和精子质量生物标志物的潜力。通过 RT-PCR 和 RT-qPCR 检测,我们确定了人类精子中来自人类 BOULE 基因的七种环状 RNA。我们发现,与对照组相比,精子circEx3-6 RNA在无精子症中的表达明显下降,而circEx2-6和circEx2-7在畸形精子症中的表达则有所下降。此外,circEx2-6的表达与精子DNA碎片指数(DFI)呈负相关,而circEx2-7的水平与辅助生殖技术的受精率和卵裂率相关。在转基因苍蝇模型中进行的进一步功能分析支持了 circBOULE RNA 在精子发育和人类生育中的作用。总之,我们的研究结果支持精子环状BOULE RNA可作为评估精子活力和DNA质量的诊断生物标志物。因此,精子环状RNA在辅助生殖技术中的临床应用和意义值得进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Human circBOULE RNAs as potential biomarkers for sperm quality and male infertility.

Reliable molecular biomarkers to predict fertility remain scarce. The current study explored the potential of testis-specific circBOULE RNAs as biomarkers for male infertility and sperm quality. Using RT-PCR and RT-qPCR assays, we identified seven circular RNAs from the human BOULE gene in human sperm. We found that sperm circEx3-6 RNA exhibited a significantly decreased expression in asthenozoospermia while circEx2-6 and circEx2-7 expression decreased in teratozoospermia, compared with the controls. Furthermore, circEx2-6 expression exhibited a negative correlation with sperm DNA Fragmentation Index (DFI), and circEx2-7 levels were correlated with both fertilization and cleavage rates involving assisted reproductive technologies. Further functional analyses in a transgenic fly model lent support for the roles of circBOULE RNAs in sperm development and human fertility. Collectively, our findings support that sperm circBOULE RNAs may serve as diagnostic biomarkers for assessing sperm motility and DNA quality. Hence clinical application and significance of sperm circular RNAs in assisted reproductive technologies warrant further investigation.

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来源期刊
Journal of Biomedical Research
Journal of Biomedical Research MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
4.60
自引率
0.00%
发文量
69
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