Journal of Advanced Research最新文献

{"title":"D-Mannose-Mediated metabolic pathways sustain the molecular signatures of sperm function and fertilization","authors":"Wenlong Zhao ,&nbsp;Nihao Gu ,&nbsp;Xueyuan Liu ,&nbsp;Ningxin Qing ,&nbsp;Jianzhong Sheng ,&nbsp;Xianhua Lin ,&nbsp;Hefeng Huang","doi":"10.1016/j.jare.2024.12.035","DOIUrl":"10.1016/j.jare.2024.12.035","url":null,"abstract":"<div><h3>Introduction</h3><div>Mammalian sperm within a single ejaculate exhibit significant heterogeneity, with only a subset possessing the molecular characteristics required for successful fertilization. Identifying the defining traits of these high-fertility sperm remains an open question.</div></div><div><h3>Objectives</h3><div>To elucidate the molecular markers and mechanisms underlying the fertilization potential of sperm in both mice and humans, with a focus on the role of D-mannose.</div></div><div><h3>Methods</h3><div>Sperm morphology and functionality were analyzed using flow cytometry, biochemical assays, and immunofluorescence. Multi-omics analyses, including proteomics, metabolomics, and lipidomics, were conducted to identify distinct molecular signatures. Pharmacological interventions were employed to validate the role of key pathways, particularly Akt/mTOR signaling.</div></div><div><h3>Results</h3><div>Sperm with longer flagella demonstrated enhanced motility, mitochondrial activity, and fertilization potential in both mice and humans. Multi-omics analyses revealed distinct molecular profiles in high-fertility sperm, characterized by specific proteins, lipids, and metabolites. Notably, D-mannose supplementation enhanced sperm motility and fertilization capacity, even in asthenozoospermic sperm, by activating the Akt/mTOR pathway. This effect was not replicated by D-glucose or ATP supplementation. Mechanistically, D-mannose bypassed glycolytic rate-limiting steps, increasing ATP production and promoting mitochondrial and acrosomal integrity.</div></div><div><h3>Conclusion</h3><div>This study identifies key molecular signatures of fertilization-competent sperm and highlights D-mannose as a novel modulator of sperm quality and function. These findings provide valuable insights into sperm biology and propose innovative therapeutic strategies for treating male infertility and optimizing assisted reproduction technologies.</div></div>","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"76 ","pages":"Pages 251-269"},"PeriodicalIF":13.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142888402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adiponectin deficiency prevents chronic colitis-associated colonic fibrosis via inhibiting CXCL13 production","authors":"Haitao Xiao ,&nbsp;Tianhang Xing ,&nbsp;Miao Qiu ,&nbsp;Guangtao Zhang ,&nbsp;Gongli Yang ,&nbsp;Wenke Chen ,&nbsp;Die Hu ,&nbsp;Deao Xue ,&nbsp;Jiao Peng ,&nbsp;Bin Du","doi":"10.1016/j.jare.2024.12.032","DOIUrl":"10.1016/j.jare.2024.12.032","url":null,"abstract":"<div><h3>Introduction</h3><div>Colonic fibrosis is a long-term complication of inflammatory bowel disease (IBD), often leading to functional impairment, intestinal obstruction, and surgery. Adiponectin (APN) is an adipokine derived from adipocytes that plays a pleiotropic role in fibrosis regulation, depending on tissue and cell type specific or disease context, but its role in colonic fibrosis remains unclear.</div></div><div><h3>Objective</h3><div>To explore the role and involved mechanism of APN in chronic colitis-associated colonic fibrosis.</div></div><div><h3>Methods</h3><div>Studies were performed in GEO database, colonic tissues of UC patients, dextran sulfate sodium (DSS)-induced colonic fibrosis in male wild-type (WT) and APN^-/- mice, mouse L929 and human CCD-18Co fibroblasts treated with recombinant CXCL13 protein, and colonic fibrosis in WT mice infected with shRNA of CXCL13.</div></div><div><h3>Results</h3><div>APN was highly expressed in the colonic tissues of UC patients and positively correlated with the colonoscopy score and colonic fibrosis markers COL1A1 and COL3A1. APN deficiency significantly improved chronic colitis-induced colonic fibrosis in mice with down-regulating collagenase accumulation and expressions of TGF-β, α-SMA, COL1A1, COL3A1, and MMP-9 in colonic tissues. Transcriptomics showed that APN deficiency mainly affected cytokine-cytokine receptor interactions, especially CXCL13 signaling. Follow-up studies showed that APN deficiency significantly decreased the number of colonic F4/80⁺CD206⁺CXCL13⁺ macrophages by weakening Akt phosphorylation. Additional experiments confirmed that CXCL13 notably increased the expressions of α-SMA and COL1A1 in mouse and human fibroblasts by activating p-Akt, p-p38, p-ERK, and p-JNK. Moreover, inhibiting CXCL13 with shRNA significantly ameliorated colonic fibrosis in mice with DSS-induced chronic colitis. Immunohistochemistry analysis revealed high expression of CXCL13 in the colon tissues of patients with UC, showing a positive correlation with APN, COL1A1, and COL3A1.</div></div><div><h3>Conclusion</h3><div>APN contributes to the progression of colonic fibrosis and can exacerbate this condition by regulating the secretion of CXCL13 in the colon, offering potential new perspectives on the pathophysiology of colonic fibrosis.</div></div>","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"76 ","pages":"Pages 639-653"},"PeriodicalIF":13.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142884686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CD34+CLDN5+ tumor associated senescent endothelial cells through IGF2-IGF2R signaling increased cholangiocellular phenotype in hepatocellular carcinoma","authors":"Xin-yu Zhu ,&nbsp;Wen-ting Liu ,&nbsp;Xiao-juan Hou ,&nbsp;Chen Zong ,&nbsp;Wei Yu ,&nbsp;Zhe-min Shen ,&nbsp;Shu-ping Qu ,&nbsp;Min Tao ,&nbsp;Meng-meng Xue ,&nbsp;Dao-yu Zhou ,&nbsp;Hao-ran Bai ,&nbsp;Lu Gao ,&nbsp;Jing-hua Jiang ,&nbsp;Qiu-dong Zhao ,&nbsp;Li-xin Wei ,&nbsp;Xue Yang ,&nbsp;Zhi-peng Han ,&nbsp;Li Zhang","doi":"10.1016/j.jare.2024.12.008","DOIUrl":"10.1016/j.jare.2024.12.008","url":null,"abstract":"<div><h3>Introduction</h3><div>The heterogeneity of hepatocellular carcinoma (HCC) is linked to tumor malignancy and poor prognosis. Nevertheless, the precise mechanisms underlying the development of the cholangiocellular phenotype (CCA) within HCC remain unclear. Emerging studies support that the cross-talk among the host cells within tumor microenvironment (TME) sustains the cancer cell plasticity.</div></div><div><h3>Objectives</h3><div>This study sought to identify the specific cell types involved in the formation of CCA and to elucidate their functional roles in the progression of HCC.</div></div><div><h3>Methods</h3><div><strong>S</strong>ingle-cell RNA sequencing was employed to identify the specific cell types involved in the formation of CCA. Both in vitro and vivo analyses were used to identify the tumor-associated senescent ECs and investigate the function in TME. The diethylnitrosamine-induced model was utilized to investigate the interaction between senescent ECs and MSCs, aiming to elucidate their synergistic contributions to the progression of CCA.</div></div><div><h3>Results</h3><div>Using single-cell RNA sequencing, we identified a distinct senescent-associated subset of endothelial cells (ECs), namely CD34⁺CLDN5⁺ ECs, which mainly enriched in tumor tissue. Further, the senescent ECs were observed to secrete IGF2, which recruited mesenchymal stem cells (MSCs) into the TME through IGF2R/MAPK signaling. In primary liver cancer model, MSCs exhibited a strong tumor-promoting effect, increasing the CCA and tumor malignancy after HCC formation. Interestingly, knockdown of IGF2R expression in MSCs inhibited the increase of CCA caused by MSCs in HCC. Meanwhile, it was revealed that MSCs released multiple inflammatory and trophic-related cytokines to enhance the cancer stem cell-like characteristics in HCC cells. Finally, we demonstrated that CEBPβ up-regulated IGF2 expression in tumor senescent ECs by combining with <em>Igf2</em>-promtor-sequence.</div></div><div><h3>Conclusions</h3><div>Together, our findings illustrated that tumor associated senescent ECs in HCC recruited the MSCs into TME, enhancing cancer stem cell (CSC)-like features of HCC cells and contributing to the CCA formation.</div></div>","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"76 ","pages":"Pages 511-528"},"PeriodicalIF":13.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive value of remnant cholesterol inflammatory index for stroke risk: Evidence from the China health and Retirement Longitudinal study","authors":"Jiaying Chen ,&nbsp;Qiheng Wu ,&nbsp;Haotian Liu ,&nbsp;Weike Hu ,&nbsp;JiaJia Zhu ,&nbsp;Zhong Ji ,&nbsp;Jia Yin","doi":"10.1016/j.jare.2024.12.015","DOIUrl":"10.1016/j.jare.2024.12.015","url":null,"abstract":"<div><h3>Introduction</h3><div>Remnant cholesterol (RC) and high-sensitivity C-reactive protein (hs-CRP) are established stroke risk factors, but their joint impact remains unclear.</div></div><div><h3>Objectives</h3><div>This study aimed to evaluate the predictive value of the remnant cholesterol inflammatory index (RCII), a novel index integrating RC and hs-CRP, in assessing stroke risk.</div></div><div><h3>Methods</h3><div>We analyzed 9,898 participants aged 45 years or older, with no history of stroke at baseline, from the China Health and Retirement Longitudinal Study (CHARLS). RCII was calculated using the formula: RCII = RC (mg/dL) × hs-CRP(mg/L)/10. A subset of 5,704 participants was studied to investigate the relationship between cumulative RCII exposure and stroke incidence. The associations of both baseline and cumulative RCII with stroke risk were assessed using Cox proportional hazards regression model.</div></div><div><h3>Results</h3><div>During a median 7-year follow-up, 560 participants (5.7 %) experienced an incident stroke. Stroke incidence escalated with increasing RCII quartiles, from 3.5 % (Q1) to 7.6 % (Q4). In multivariable-adjusted analyses, each standard deviation increase in RCII was significantly associated with a 10.6 % increased risk of stroke (HR = 1.106, 95 % CI: 1.048–1.167). ROC analysis revealed that RCII had the highest AUC at 0.581, higher than RC (0.566) and hs-CRP (0.560), though the difference with RC was not statistically significant (P = 0.166). Mediation analysis indicated a reciprocal mediation between RC and hs-CRP on stroke risk. In a 3-year subset analysis, 288 participants suffered a stroke. Participants with cumulative RCII levels exceeding 36.14 had a significantly increased risk of incident stroke (HR = 1.462, 95 % CI: 1.102–1.939). Subgroup analyses showed a significant positive association between elevated RCII levels and stroke risk in males, but not in females.</div></div><div><h3>Conclusions</h3><div>Elevated levels of RCII, both at baseline and cumulative, are significantly associated with an increased risk of stroke. Early intervention in patients with high RCII may further help reduce stroke risk.</div></div>","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"76 ","pages":"Pages 543-552"},"PeriodicalIF":13.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loss of glomerular aldolase B in diabetic nephropathy promotes renal fibrosis via activating Akt/GSK/β-catenin axis","authors":"Minghui Liu ,&nbsp;Wenwen Yang ,&nbsp;Shuang Qu ,&nbsp;Tingting Zhao ,&nbsp;Song Jiang ,&nbsp;Suming Peng ,&nbsp;Mingchao Zhang ,&nbsp;Ji Xuan ,&nbsp;Zhihong Liu ,&nbsp;Ke Zen","doi":"10.1016/j.jare.2024.12.027","DOIUrl":"10.1016/j.jare.2024.12.027","url":null,"abstract":"<div><h3>Objective</h3><div>Diabetic nephropathy (DN), characterized by a complex and multifaceted pathogenesis, stands as the foremost catalyst behind end-stage renal disease (ESRD). This study aims to analyze the level and non-metabolic role of glomerular aldolase B (ALDOB) in DN progression.</div></div><div><h3>Methods</h3><div>Glomerular proteomics and transcriptome are analyzed from 50 DN patients and 25 controls, respectively. Human kidney biopsy, cultured podocytes and mouse models are employed to study ALDOB levels and function.</div></div><div><h3>Results</h3><div>ALDOB is strongly downregulated in DN-affected glomeruli, as well as in human and murine podocytes exposed to inflammatory cytokines. ALDOB reduction increases podocyte injury, while adenovirus-mediated ALDOB overexpression leads to substantial alleviation of renal injuries in a diabetic mouse model. Mechanistically, ALDOB reduction triggers the Akt/GSK/β-catenin signaling cascade within podocytes.</div></div><div><h3>Conclusion</h3><div>Our findings reveal a novel non-metabolic role of glomerular ALDOB in protecting against podocyte injury and renal fibrosis.</div></div>","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"76 ","pages":"Pages 207-218"},"PeriodicalIF":13.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142884688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lonicera caerulea genome reveals molecular mechanisms of freezing tolerance and anthocyanin biosynthesis","authors":"Jing Wang ,&nbsp;Xiaotong Wang ,&nbsp;Yuwei Ma ,&nbsp;Ranran Gao ,&nbsp;Yongmiao Wang ,&nbsp;Zhoujie An ,&nbsp;Ya Tian ,&nbsp;Huihua Wan ,&nbsp;Dianwen Wei ,&nbsp;Feng Wang ,&nbsp;Baojiang Zheng ,&nbsp;Baozhong Duan ,&nbsp;Li Xiang ,&nbsp;Gangqiang Dong ,&nbsp;Wei Sun ,&nbsp;Zhichao Xu","doi":"10.1016/j.jare.2024.12.038","DOIUrl":"10.1016/j.jare.2024.12.038","url":null,"abstract":"<div><h3>Introduction</h3><div><em>Lonicera caerulea</em> L. (blue honeysuckle) is a noteworthy fleshy-fruited tree and a prominent medicinal plant, which possesses notable characteristics such as exceptional resilience to winter conditions and early maturation, and the richest source of functional anthocyanins, particularly cyanidin-3-glucoside. The molecular mechanisms responsible for its freezing tolerance and anthocyanin biosynthesis remain largely unknown.</div></div><div><h3>Objectives</h3><div>Here, a chromosome-scale genome of <em>L. caerulea</em> was presented, aiming to examine the genetic foundations that underlie these characteristics of blue honeysuckle.</div></div><div><h3>Methods</h3><div>The PacBio HiFi reads and Hi-C data were used to construct high-quality genome of blue honeysuckle. Comparative genomic and transcriptomic analyses were conducted to elucidate the molecular mechanisms of freezing tolerance and anthocyanin biosynthesis.</div></div><div><h3>Results</h3><div>Comparative genomics analysis between <em>L. caerulea</em> and <em>L. japonica</em> revealed that the dynamic changes of duplicated genes contributed to their phytochemical reconstruction and environmental adaptation. Moreover, the ABA and ICE-CBF-COR signaling pathways were closely correlated to the freezing tolerance of <em>L. caerulea</em>. Genome-wide identification and biochemical function indicated that three anthocyanin 3′,5′-<em>O</em>-methyltransferases (<em>LcOMT2</em>, <em>LcOMT14</em>, and <em>LcOMT20</em>) and two 3′-<em>O</em>-glycosyltransferases (<em>LcUGT78X1</em> and <em>LcUGT95P1</em>) were responsible for anthocyanin biosynthesis. In addition, <em>LcUGT78X1</em> was regarded as the potent glycosyltransferase for the accumulation of cyanidin-3-glucoside in <em>L. caerulea</em>.</div></div><div><h3>Conclusion</h3><div>This research elucidates the crucial roles of the ABA and ICE-CBF-COR signaling pathways in enhancing freezing tolerance, while also identifying highly efficient anthocyanin biosynthetic enzymes in <em>L. caerulea</em>. These findings advance the understanding of environmental adaptation and phytochemical production in <em>Lonicera</em> species.</div></div>","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"76 ","pages":"Pages 293-305"},"PeriodicalIF":13.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of cGAS-STING signaling pathway in ferroptosis","authors":"Lina Ding ,&nbsp;Ruicheng Zhang ,&nbsp;Wenqi Du ,&nbsp;Qingling Wang ,&nbsp;Dongsheng Pei","doi":"10.1016/j.jare.2024.12.028","DOIUrl":"10.1016/j.jare.2024.12.028","url":null,"abstract":"<div><div>The cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signaling pathway has been identified as a crucial mechanism in antiviral defense and innate immunity pathway. Ferroptosis, characterized by iron dependence and lipid peroxidation, represents a specialized form of cell death. A burgeoning collection of studies has demonstrated that the cGAS-STING signaling pathway participates in the homeostatic regulation of the organism by modulating ferroptosis-associated enzyme activity or gene expression. Consequently, elucidating the specific roles of the STING signaling pathway and ferroptosis in vivo is vital for targeted disease intervention. This review systematically examines the interactions between the cGAS-STING signaling pathway and ferroptosis, highlighting their influence on disease progression in the contexts of inflammation, injury, and cancerous cell dynamics. Understanding these interactions may provide novel therapeutic strategies. The STING pathway has been implicated in the regulation of various cell death mechanisms, including apoptosis, pyroptosis, necroptosis, autophagy, and ferroptosis. Our focus primarily addresses the role and mechanism of the cGAS-STING signaling pathway and ferroptosis in diseases, limiting discussion of other cell death modalities and precluding a comprehensive overview of the pathway’s additional functions.</div></div>","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"76 ","pages":"Pages 219-231"},"PeriodicalIF":13.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving grain yield and salt tolerance by optimizing plant height with beneficial haplotypes in rice (Oryza sativa)","authors":"Ruidang Quan ,&nbsp;Juan Wang ,&nbsp;Hua Qin ,&nbsp;Liang Chen ,&nbsp;Dinglin Xiao ,&nbsp;Zihan Zhao ,&nbsp;Zhanying Zhang ,&nbsp;Xiaoyang Zhu ,&nbsp;Zichao Li ,&nbsp;Rongfeng Huang","doi":"10.1016/j.jare.2024.12.007","DOIUrl":"10.1016/j.jare.2024.12.007","url":null,"abstract":"<div><h3>Introduction</h3><div>Rice (<em>Oryza sativa</em> L.), a staple food for billions worldwide, is challenged by salt stress. Owing to the limited understanding of the physiological and genetic basis of rice salt tolerance, few genes have been identified as valuable in rice breeding, causing a major bottleneck in the development of high-yield, salt-tolerant rice varieties.</div></div><div><h3>Objective</h3><div>This study aims to identify salt tolerance genes/quantitative trait loci (QTLs) with breeding potential in rice.</div></div><div><h3>Methods</h3><div>Field trials were conducted with 166 Chinese rice cultivars from saline-affected regions and 412 global rice accessions to assess salt tolerance. Genome-wide association study (GWAS) was performed to identify key loci related to high yield and salt tolerance. Additionally, the impact of introducing beneficial haplotypes on grain yield and salt tolerance was assessed.</div></div><div><h3>Results</h3><div>The optimal rice plant height of 100–120 cm was crucial for sustaining high yield under both normal and salt stress conditions. GWAS revealed 6 novel QTLs/genes associated with rice plant growth and grain yield across various environments, distinct from previously recognized salt stress-related genes. Notably, the gene <em>PHS10.1</em>, encoding a serine/threonine protein kinase, may regulate carbon metabolism, starch and sucrose metabolism, influencing plant growth and grain yield. Certain haplotypes of the genes regulating plant height and grain yield, including <em>SD1</em>, <em>Ghd7.1</em>, <em>GH3.5,</em> and <em>PHS10.1</em>, were selected in traditional breeding. Moreover, optimizing plant height through the introgression of beneficial alleles of these genes increased grain yield in recipient lines under both normal and saline conditions.</div></div><div><h3>Conclusion</h3><div>We propose that utilizing beneficial haplotypes to optimize plant height can effectively balance the growth–stress trade-offs in rice plants. This represents a promising breeding strategy for the development of crop varieties that are both high-yielding and salt-tolerant.</div></div>","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"76 ","pages":"Pages 17-32"},"PeriodicalIF":13.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transient receptor potential channels in viral infectious diseases: Biological characteristics and regulatory mechanisms","authors":"Wen-Hui Qi ,&nbsp;Na Tang ,&nbsp;Zhi-Jing Zhao ,&nbsp;Xiao-Qiang Li","doi":"10.1016/j.jare.2024.11.022","DOIUrl":"10.1016/j.jare.2024.11.022","url":null,"abstract":"<div><h3>Background</h3><div>Viral infectious diseases have long posed a challenge to humanity. In recent decades, transient receptor potential (TRP) channels have emerged as newly investigated cation channels. Increasing evidence suggests that TRP channel-mediated Ca²⁺ homeostasis disruptions, along with associated pathological changes, are critical factors in the onset and progression of viral infectious diseases. However, the precise roles and mechanisms of TRP channels in these diseases remain to be systematically elucidated.</div></div><div><h3>Aim of Review</h3><div>The aim of this review is to systematically summarize recent advances in understanding TRP channels in viral infections, and based on current progress and challenges, propose future directions for research.</div></div><div><h3>Key Scientific Concepts of Review</h3><div>This review summarizes the classification and biological functions of the TRP family, explores the mechanisms by which TRP channels contribute to viral infections, and highlights specific mechanisms at three levels: virus, host, and outcome. These include the direct role in viral biology and replication, the indirect role in host immunity and inflammation, and the resulting pathological changes. Additionally, we discuss the potential applications of the TRP family in the treatment of viral infectious diseases and propose future research directions.</div></div>","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"76 ","pages":"Pages 119-133"},"PeriodicalIF":13.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142650442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-adhesion conductive cardiac patch based on methoxytriethylene glycol-functionalized graphene effectively improves cardiac function after myocardial infarction","authors":"Xu Wang ,&nbsp;Hao Wang ,&nbsp;Xin Liu ,&nbsp;Yuan Zhang ,&nbsp;Jiamin Li ,&nbsp;Heng Liu ,&nbsp;Jing Feng ,&nbsp;Wenqian Jiang ,&nbsp;Ling Liu ,&nbsp;Yongchao Chen ,&nbsp;Xiaohan Li ,&nbsp;Limin Zhao ,&nbsp;Jing Guan ,&nbsp;Yong Zhang","doi":"10.1016/j.jare.2024.11.026","DOIUrl":"10.1016/j.jare.2024.11.026","url":null,"abstract":"<div><h3>Introduction</h3><div>Abnormal electrical activity of the heart following myocardial infarction (MI) may lead to heart failure or sudden cardiac death. Graphene-based conductive hydrogels can simulate the microenvironment of myocardial tissue and improve cardiac function post-MI. However, existing methods for preparing graphene and its derivatives suffer from drawbacks such as low purity, complex processes, and unclear structures, which limiting their biological applications.</div></div><div><h3>Objectives</h3><div>We propose an optimized synthetic route for synthesizing methoxytriethylene glycol-functionalized graphene (TEG-GR) with a defined structure. The aim of this study is to establish a novel self-adhesion conductive cardiac patch based on TEG-GR for protecting cardiac function after MI.</div></div><div><h3>Methods</h3><div>We optimized π-extension polymerization (APEX) reaction to synthesize TEG-GR. TEG-GR was incorporated into dopamine-modified gelatin (GelDA) to construct conductive cardiac patch (TEG-GR/GelDA). We validated the function of TEG-GR/GelDA cardiac patch in rat models of MI, and explored the mechanism of TEG-GR/GelDA cardiac patch by RNA sequencing and molecular biology experiments.</div></div><div><h3>Results</h3><div>Methoxytriethylene glycol side chain endowed graphene with low immunogenicity and superior biological properties without compromising conductivity. In rats, transplantation of TEG-GR/GelDA cardiac patch onto the infarcted area of heart could more effectively enhance ejection fraction, attenuate collagen deposition, shorten QRS interval and increase vessel density at 28 days post-treatment, compared to non-conductive cardiac patch. Transcriptome analysis indicated that TEG-GR/GelDA cardiac patch could improve cardiac function by maintaining gap junction, promoting angiogenesis, and suppressing cardiomyocytes apoptosis.</div></div><div><h3>Conclusion</h3><div>The precision synthesis of polymer with defined functional group expands the application of graphene in biomedical field, and the novel cardiac patch can be a promising candidate for treating MI.</div></div>","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"76 ","pages":"Pages 745-759"},"PeriodicalIF":13.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142671092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}