Journal of Advanced Research最新文献

{"title":"Physiological and pathological functions of TAF15 in neurodegenerative diseases and cancers","authors":"Congcong Liu, Lanxia Meng, Zhentao Zhang","doi":"10.1016/j.jare.2026.04.066","DOIUrl":"https://doi.org/10.1016/j.jare.2026.04.066","url":null,"abstract":"<h3>Background</h3>TATA-box binding protein associated factor 15 (TAF15) is a multifunctional DNA/RNA-binding protein that plays pivotal roles in transcription regulation, precursor mRNA splicing, and cellular stress responses. Accumulating evidence demonstrates that TAF15 is strongly implicated in two distinct pathological classes: neurodegenerative diseases and cancers. In neurodegenerative diseases including frontotemporal lobar degeneration (FTLD) and amyotrophic lateral sclerosis (ALS), TAF15 undergoes abnormal cytoplasmic aggregation and mislocalization in neurons and glia, and TAF15 has been established as a candidate disease gene for ALS. In a wide range of cancers, TAF15 drives oncogenic transcriptional dysregulation either via wild-type protein dysfunction or the formation of oncogenic fusion proteins derived from chromosomal translocations.<h3>Aim of review</h3>A central unresolved question is how TAF15 contributes to two mechanistically distinct disease entities. This review aims to provide a mechanistically integrated analysis of the physiological and pathological functions of TAF15. We use TAF15′s intrinsic molecular properties as a unifying framework to connect its roles in neurodegeneration and cancer. We also summarize key pathogenic mechanisms and emerging therapeutic strategies targeting TAF15, with the goal of proposing a novel conceptual perspective to guide future research.Key scientific concepts of review.TAF15 may act as a biologically relevant molecular link between neurodegeneration and cancer through its intrinsic molecular characteristics, such as nucleic acid binding, phase separation, and nucleocytoplasmic shuttling. The “localization determines outcome” hypothesis offers a unifying framework to explain the connection between the two diseases. TAF15 holds promise as a target for novel biomarkers and precision therapeutics across both disease areas. Deepening mechanistic studies of TAF15 will not only advance understanding of its dual pathological roles but also illuminate the largely unexplored molecular link between neurodegenerative diseases and cancers","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"144 1","pages":""},"PeriodicalIF":10.7,"publicationDate":"2026-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147751663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rutin Combats Salmonella infection through virulence suppression and gut barrier maintenance","authors":"Tao Wang, Xinyou Zhang, Haihua Feng, Jianfeng Wang, Xuming Deng, Jinli Ge, Hongtao Liu, Jiazhang Qiu","doi":"10.1016/j.jare.2026.04.061","DOIUrl":"https://doi.org/10.1016/j.jare.2026.04.061","url":null,"abstract":"<h3>Introduction</h3><em>Salmonella</em> Typhimurium (<em>S.</em> Typhimurium) is a major zoonotic pathogen causing severe gastroenteritis and septicemia. Traditional antibiotics targeting bacterial growth face increasing resistance challenges, while the development of novel antimicrobials is slow and costly. Antivirulence therapy, which targets bacterial virulence factors, has emerged as a promising alternative strategy.<h3>Objectives</h3>To demonstrate that the natural flavonoid rutin can serve as an antivirulence agent against <em>S.</em> Typhimurium infection.<h3>Methods</h3>A host-cell invasion assay was used to screen natural compounds. Bacteria treated with rutin were subjected to transcriptomic profiling (RNA-seq), followed by validation using qPCR, Western blot, a β-lactamase reporter system, intrinsic fluorescence spectroscopy, circular dichroism and electrophoretic mobility shift assays. Auto-aggregation and crystal-violet assays, together with confocal and scanning electron microscopy, were used to evaluate biofilm-associated phenotypes. An acute murine salmonellosis model was used to evaluate the therapeutic efficacy of rutin. Histological analysis, immunostaining and <em>16S</em> rRNA gene sequencing were performed to assess intestinal barrier integrity and gut microbiota composition.<h3>Results</h3>Rutin significantly inhibited <em>S.</em> Typhimurium invasion of HeLa and Caco-2 cells without affecting bacterial growth or host cell viability. Integrated transcriptomic and functional analyses demonstrated that rutin targets HilA, the master regulator of type III secretion system 1 (T3SS-1), leading to reduced T3SS-1 effector expression and secretion. Rutin also decreased transcription of the biofilm master regulator CsgD, reduced bacterial auto-aggregation, and disrupted mature biofilm architecture. In infected mice, oral administration of rutin decreased bacterial burden and tissue injury, improved epithelial barrier integrity, attenuated intestinal inflammation, and alleviated gut microbiota imbalance.<h3>Conclusion</h3>Rutin mitigates <em>S.</em> Typhimurium infection through coordinated suppression of HilA-dependent T3SS-1 activity and CsgD-mediated biofilm formation, while supporting intestinal barrier function and modulating gut microbial composition. Rutin shows potential as an adjunct or alternative to conventional antibiotics.","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"250 1","pages":""},"PeriodicalIF":10.7,"publicationDate":"2026-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147751664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in super-resolution imaging technology bring new perspectives to cytoskeleton mechanism research","authors":"Song Peng, Ferdinand Ndikuryayo, Xue-Yan Gong, WMWW Kandegama, Wen-Chao Yang","doi":"10.1016/j.jare.2026.04.056","DOIUrl":"https://doi.org/10.1016/j.jare.2026.04.056","url":null,"abstract":"The cytoskeleton constitutes the fundamental structural framework governing cellular morphology and the regulation of intracellular cellular processes. Its nanoscale architecture and dynamics are essential for understanding the mechanisms of fundamental biological activities. However, conventional imaging techniques are constrained by Abbe diffraction limit, precluding the visualization of cytoskeleton at nanoscale. While electron microscopy (EM) offers superior resolution, it is intrinsically constrained by a lack of live-cell compatibility and restricted three-dimensional (3D) resolving power. These technical bottlenecks have long hindered the in-depth dissection of cytoskeleton regulatory mechanisms. Super-resolution microscopy (SRM) has emerged as transformative paradigm, circumventing the diffraction barrier to enable high-resolution imaging of ultrastructural components and real-time tracking within living systems. This review provides a comprehensive synthesis of the development of mainstream SRM modalities—especially Stimulated Emission Depletion Microscopy (STED), Structured Illumination Microscopy (SIM), and Single-Molecule Localization Microscopy (SMLM)—and their applications in investigating microfilaments, microtubules, and intermediate filaments. We delineate key biological insights facilitated by SRM, including the discovery of the membrane-associated periodic skeleton (MPS) in axons, the vimentin-mediated stabilization of microtubule dynamics, and the unimpeded walking mechanism of kinesin-1 resolved via Minimum Fluorescence Flux Microscopy (MINFLUX) nanoscopy. Furthermore, we provide a comparative analysis of the performance metrics and application niches of various SRM techniques. By summarizing SRM-driven advancements and addressing current technical issues, this work serves as a critical reference for researchers navigating SRM selection to conduct innovative cytoskeletal research.","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"115 1","pages":""},"PeriodicalIF":10.7,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147744101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nonlinear trajectories of multi-organ aging and microbial associations in mice","authors":"Fang He, Fenglin Song, Yixuan Xu, Yueying Wu, Caixia Wang, Yuheng Mao, Yunhua Guo, Dong Liang, Wenhua Ling, Yan Yang","doi":"10.1016/j.jare.2026.04.057","DOIUrl":"https://doi.org/10.1016/j.jare.2026.04.057","url":null,"abstract":"Aging is characterized by progressive, nonlinear decline across organ systems, yet the temporal coordination of multi-organ aging and its relationship with gut microbiota remodeling remain insufficiently defined within a unified framework.","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"136 1","pages":""},"PeriodicalIF":10.7,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147736822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SUMOylation-deficient TRPV1 in sensory neurons confers resistance to high-fat diet-induced obesity via enhanced sympathetic adipose innervation","authors":"Xiaokun Gu, Weiji Weng, Yang Yang, Sihan Guo, Gerald W. Zamponi, Zhenzhou He, Ou Huang, Yong Li","doi":"10.1016/j.jare.2026.04.053","DOIUrl":"https://doi.org/10.1016/j.jare.2026.04.053","url":null,"abstract":"Sensory neurons have emerged as modulators of adipose metabolism and systemic energy balance, with implications for obesity. TRPV1, a nociceptive ion channel, also participates in metabolic regulation, but its precise role remains unclear. While our prior work showed that TRPV1 SUMOylation in sensory neurons is critical for nociception and itch, its contribution to obesity through neuro–metabolic crosstalk is unknown.","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"27 1","pages":""},"PeriodicalIF":10.7,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147736824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic module exchange in plant-endophyte coevolution: mechanisms and implications","authors":"Xuejing Zhong, Hanping Bao, Shen Zhang, Yuxuan Gong, Xiaolong Li, Chunhong Zhang, Minhui Li","doi":"10.1016/j.jare.2026.04.054","DOIUrl":"https://doi.org/10.1016/j.jare.2026.04.054","url":null,"abstract":"Endophytes establish intimate symbioses with their hosts through long-term coevolution. They are known to promote plant growth, enhance stress resistance, and contribute to the biosynthesis of bioactive compounds. However, despite extensive research on these beneficial roles, a systematic synthesis of their distribution patterns, functional differentiation, and mechanisms of metabolite production remains lacking. This knowledge gap limits our understanding of plant-endophyte coadaptation and hinders its applications in agriculture and medicine. <ce:bold>Purpose of Review:</ce:bold> This review critically synthesizes major studies on endophyte diversity, functional specialization, and metabolite biosynthesis. By integrating bibliometric and systematic evidence, we aim to elucidate the essential patterns and possible mechanisms underlying these phenomena-specifically, how endophytes and their host plants achieve functional complementarity and metabolic congruence. We further evaluate the roles of horizontal gene transfer and convergent evolution in enabling both partners to produce identical or structurally similar bioactive metabolites. <ce:bold>Significant Scientific Ideas:</ce:bold> Endophyte communities are highly structured, forming the basis for their interactions with host plants. Their distribution and functions are host‑ and tissue‑specific: strains from the same tissue of congeneric species show closer phylogenetic relatedness. A clear functional division exists in phytohormone production-bacteria predominantly produce indole-3-acetic acid (IAA), while fungi mainly produce gibberellins (GAs). Roots represent the dominant and most diverse colonization site. Horizontal gene transfer and convergent evolution serve as critical mechanisms enabling metabolic complementation between endophytes and plants. Together, these findings indicate that plant–endophyte coadaptation is underpinned by structured community assembly and functional specialization, rather than by random association.","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"19 1","pages":""},"PeriodicalIF":10.7,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147736823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone marrow mesenchymal stem cells have tissue-specific characteristic in promoting the potential of periodontal ligament stem cells for periodontal regeneration","authors":"Fen Liu, Zhifei Zhou, Xiao Sun, Bin Zhu, Shaoqing Yang, Xuan Li, Zhou Zhang, Ying An, Faming Chen","doi":"10.1016/j.jare.2026.04.058","DOIUrl":"https://doi.org/10.1016/j.jare.2026.04.058","url":null,"abstract":"Periodontal ligament stem cells (PDLSCs) are commonly used in regenerating damaged periodontal tissues. The regeneration of complex periodontal tissues relies on stem cell crosstalk. Typically, bone marrow mesenchymal stem cells (BMMSCs) are used to promote the differentiation of PDLSCs. However, it is currently unknown whether BMMSCs have tissue-specific characteristic in promoting the differentiation of PDLSCs.","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"244 1","pages":""},"PeriodicalIF":10.7,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147736830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary EGCG reshapes metabolic-epigenetic interplay to induce transgenerational host defense","authors":"Wenqi Huang, Shiye Lin, Xuanyu Zheng, Mohamed A. Farag, Thomas Efferth, Jesus Simal-Gandara, Zimiao Chen, Jianbo Xiao, Hui Cao","doi":"10.1016/j.jare.2026.04.028","DOIUrl":"https://doi.org/10.1016/j.jare.2026.04.028","url":null,"abstract":"<h3>Introduction</h3>Parental diet is a key determinant of offspring health and immune function, in part through epigenetic regulation. Metabolic and epigenetic networks integrate nutrient sensing with chromatin dynamics to maintain cellular and organismal homeostasis. However, the mechanism by which specific dietary bioactive compounds reshape metabolic-epigenetic networks to drive transgenerational adaptive responses remains poorly understood.<h3>Objectives</h3>Here, we investigate whether and how epigallocatechin-3-gallate (EGCG), a well-characterized dietary bioactive compound, modulates heritable host defense through metabolic-epigenetic crosstalk.<h3>Methods</h3>To address both physiological relevance and mechanistic insight, we employed mouse and <em>Drosophila melanogaster</em> models. Parental animals were administered EGCG, and offspring were subsequently assessed for immune function upon infection with <em>Escherichia coli</em>, <em>Pseudomonas aeruginosa</em>, or <em>Staphylococcus aureus</em>. By integrating transcriptomics, metabolite analysis, and isotopic tracing, we analyzed metabolism-related pathways and constructed a dynamic network linking metabolic changes to epigenetic modifications in <em>Drosophila</em>.<h3>Results</h3>In mice, EGCG administration led to a decrease in <em>Escherichia coli</em> burden across multiple tissues in paternal and male offspring in a sex-specific manner, accompanied by metabolic and pro-inflammatory factor changes. In <em>Drosophila melanogaster</em>, early-life EGCG exposure increased survival upon <em>Pseudomonas aeruginosa</em> or <em>Staphylococcus aureus</em> infection and persisted for two subsequent generations. Mechanistically, EGCG reduced intestinal amino acids, thereby moderately inducing activation of activating transcription factor 4 (ATF4), which in turn enhanced maternal glycolysis and immune adaptation. Tyrosine supplementation abolished the enhanced host defense and metabolic changes. Furthermore, ATF4-induced activation of glycolysis promoted ovarian lactate production, serving as a substrate for increased global H3K27 acetylation in the offspring.<h3>Conclusion</h3>Together, these findings suggest that dietary bioactive compounds modulate metabolic and gene regulatory processes, with functional evidence supporting a role for amino acid metabolism and lactate in linking metabolic remodeling to enhanced resistance to infection in the offspring. This work provides mechanistic insight into how diet can shape heritable immune function through metabolic-epigenetic interplay","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"27 1","pages":""},"PeriodicalIF":10.7,"publicationDate":"2026-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147719646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Time-series single-cell transcriptomics and spatial metabolomics reveal spatiotemporal tobacco leaf response to herbivory","authors":"Huan Su, Xiaoxiang Hu, Lijun Meng, Xinhua Tian, Xiaoyu Zhang, Qiao Wang, Jiemeng Tao, Peng Lu, Jizhong Wu, Huabing Liu, Kaiyan Li, Peijian Cao, Jingjing Jin","doi":"10.1016/j.jare.2026.04.048","DOIUrl":"https://doi.org/10.1016/j.jare.2026.04.048","url":null,"abstract":"Introduction: Plants have evolved complex defense systems to cope with herbivore attack, yet how these defense responses are coordinated across distinct cell types and time scales remains unclear. Understanding the cellular and spatial organization of such defenses is critical for elucidating the mechanisms underlying plant–insect interactions.Objectives: This study aimed to dissect the spatiotemporal regulation of tobacco leaf defense responses to herbivory by integrating time-series single-cell transcriptomics and spatial metabolomics.Methods: We generated a high-resolution single-cell atlas of 28,318 tobacco leaf cells under simulated herbivory. Transcriptional dynamics, cell–cell communication networks, and spatial metabolite distributions were analyzed using co-expression, pseudotime, and ligand–receptor inference approaches. Functional validation of key regulators was conducted through qRT-PCR and VIGS assays.Results: Our analyses revealed rapid transcriptional and metabolic remodeling following herbivore stress, with epidermal subpopulations serving as early signaling hubs. Spatial metabolomics confirmed epidermis-enriched accumulation of defense metabolites. Pseudotime and co-expression analyses identified epidermal subcluster 6 as an early-responsive population characterized by elevated <em>WRKY81</em> expression. Silencing <em>WRKY81</em> impaired defense activation, increasing herbivore feeding efficiency and metabolic assimilation.Conclusion: This study establishes the first single-cell–resolved dynamic defense network of tobacco leaves against herbivory. The findings uncover the central role of epidermal transcriptional reprogramming and identify <em>WRKY81</em> as a critical regulator of early defense commitment, offering a mechanistic basis for the development of pest-resistant crops.","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"126 1","pages":""},"PeriodicalIF":10.7,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147708667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nafamostat mesilate improves sepsis outcomes by modulating complement and coagulation pathways","authors":"Qiaolan He, Yuanjun Yang, Zhuxin Xie, Yuanyuan Wu, Chenghang Yang, Yiqi Qian, Ming Zhong","doi":"10.1016/j.jare.2026.04.041","DOIUrl":"https://doi.org/10.1016/j.jare.2026.04.041","url":null,"abstract":"<h3>Introduction</h3>Sepsis is a leading cause of mortality in critically ill patients, characterized by dysregulation of multiple biological systems, including the complement, coagulation and contact pathways Nafamostat (NM), a serine protease inhibitor, has shown potential in modulating these systems.<h3>Objectives</h3>We investigated the potential therapeutic role of NM in sepsis and its mechanism.<h3>Methods</h3>High-throughput mass spectrometry proteomics analysis was conducted to investigate changes in protein levels among sepsis patients. Leveraging the chemical structure of NM, multiple drug-target prediction databases were employed to forecast potential targets. In septic mice, evaluation of complement, coagulation, and contact system activation were conducted after NM administration. Plasma Kininogen 1 (KNG1) was overexpressed to validate roles in the occurrence and development of sepsis. A retrospective study was conducted to evaluate the impact of NM as an anticoagulant for continuous renal replacement therapy (CRRT) on the prognosis of septic patients.<h3>Results</h3>107 differentially expressed proteins (DEPs) were identified in sepsis patients with varying prognosis. 622 predicted targets for NM and 184 DEPs in sepsis were identified, with 16 overlapped genes enriching in complement and coagulation cascades. NM reduced plasma KNG1 cleavage and downstream products, alleviating organ damage in septic mice. In a small pilot cohort, NM reduced SOFA scores and platelet consumption while prolonging APTT compared to unfractionated heparin.<h3>Conclusion</h3>NM may offer a novel therapeutic approach by modulating dysregulated pathways, thus improving organ function and clinical outcomes in sepsis.","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"67 1","pages":""},"PeriodicalIF":10.7,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147695891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}