Journal of Animal Science and Technology最新文献

{"title":"Dietary flavor supplementation enhanced growth performance and alleviated diarrhea of weaned pigs by modulating gut microbiota and systemic immunity.","authors":"Hyunjin Kyoung, Soyun Kim, Jin Ho Cho, Jeehwan Choe, Jinmu Ahn, Jinuk Nam, Yonggu Kang, Younghoon Kim, Hyeun Bum Kim, Minho Song","doi":"10.5187/jast.2025.e3","DOIUrl":"10.5187/jast.2025.e3","url":null,"abstract":"<p><p>The experiment was conducted to investigate the effects of dietary flavor on growth performance, diarrhea severity, nutrient digestibility, systemic immunity, and gut microbiota of weaned pigs. Weaned pigs (n = 72; 6.66 ± 0.32 kg body weight [BW]; 28 days of age) were randomly assigned to one of the two dietary treatments (9 pens/treatment; 4 pigs/pen): nursery basal diet (CON) and CON supplemented with 0.05% milky cream flavor (FLA). The experiment lasted for 42 days. Pigs in the FLA group tended to have increase (<i>p</i> < 0.10) BW on day 42 and had greater (<i>p</i> < 0.05) average daily gain during each interval, compared with pigs in the CON group. Pigs fed FLA diet tended to have increase (<i>p</i> < 0.10) average daily feed intake during the overall period compared with pigs fed CON diet. The FLA group had increased (day 1 to 7, <i>p</i> < 0.05; day 1 to 14, <i>p</i> < 0.10; day 1 to 21, <i>p</i> < 0.05) gain to feed ratio during each interval compared with the CON group, except from day 1 to 42. The FLA group showed reduced (<i>p</i> < 0.05) frequency of diarrhea from day 8 to 14 after weaning and tended to increase (<i>p</i> < 0.10) gross energy apparent total tract digestibility compared with the CON group. Pigs fed FLA diet tended to have lower (<i>p</i> < 0.10) white blood cell count, serum TNF-α, and serum IL-6 on day 7 and lower (<i>p</i> < 0.05) white blood cell counts on day 14 than pigs fed CON diet. Pigs treated with FLA had a higher (<i>p</i> < 0.05) relative abundance of Firmicutes, whereas had a lower (<i>p</i> < 0.05) relative abundance of Bacteroidetes than pigs treated with CON. At the genus level, the FLA group had a higher (<i>p</i> < 0.05) relative abundance of <i>Lactobacillus</i> and <i>Clostridium</i> but had a lower (<i>p</i> < 0.05) relative abundance of <i>Prevotella</i> in fecal samples than the CON group. In conclusion, dietary flavor supplementation may enhance growth performance, reduce diarrhea, and increase energy digestibility by alleviating systemic immune responses and altering the gut microbiota of weaned pigs.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 1","pages":"168-180"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12902547/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146201843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the efficacy of <i>in vitro</i> and <i>in vivo</i> methods for assessing nutrient digestibility in Sapsarees each age.","authors":"Kyeongho Jeon, Jihwan Lee, Minho Song, Gokmi Kim, Seyeon Chang, Dongcheol Song, Hyuck Kim, Jinmo Yang, Jinho Cho","doi":"10.5187/jast.2025.e34","DOIUrl":"10.5187/jast.2025.e34","url":null,"abstract":"<p><p>This study evaluated the comparison between <i>in vitro</i> and <i>in vivo</i> methods for predicting nutrient digestibility across different life stages in Sapsarees. The research performed both <i>in vitro</i> methods of dog gastrointestinal conditions such as stomach and small intestine conditions and <i>in vivo</i> methods using 18 Sapsaree groups. Sapsarees were categorized into three groups by age and weight: six puppies (under 1 year; 9.94 ± 5.27 kg), six adult dogs (2-7 years; 23.49 ± 3.90 kg), and six senior dogs (over 8 years; 21.57 ± 2.27 kg). The nutrients examined included dry matter (DM), organic matter (OM), crude protein (CP), gross energy (GE), crude fiber (CF), and ether extract (EE). The significant differences were found in the digestibility of OM, CF, and EE between the methods (<i>p</i> < 0.05) except the digestibility of DM in puppies and adult dogs and gross energy GE digestibility. In puppies, there were strong linear relationships for OM, GE, CF, and EE with <i>R</i> ² values of 0.85, 0.90, 0.85, and 0.82, respectively, between <i>in vitro</i> and <i>in vivo</i> digestibility. Also, in adult dogs, there were strong linear relationships for DM, GE, and CF with <i>R</i> ² values of 0.85, 0.90, and 0.91, respectively, between <i>in vitro</i> and <i>in vivo</i> digestibility. In the relationship between <i>in vitro</i> and <i>in vivo</i> digestibility of senior dogs, there were strong linear relationships for OM with <i>R</i> ² values of 0.87. The <i>in vitro</i> method shows a strong correlation with <i>in vivo</i> digestibility and is predicted to have significant potential for practical application.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 1","pages":"357-365"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12902546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146201825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic profiling of porcine duodenal, jejunal, and ileal organoids in response to porcine epidemic diarrhea virus.","authors":"Jiyoung Heo, Jae Han Park, Hyun Sung Park, Dong-Hoon Chae, Aaron Yu, Keonwoo Cho, Dong Ha Bhang, Yoo Yong Kim, Mi-Kyung Oh, Kyung-Rok Yu","doi":"10.5187/jast.2024.e114","DOIUrl":"10.5187/jast.2024.e114","url":null,"abstract":"<p><p>Porcine epidemic diarrhea virus (PEDV) is a highly pathogenic virus that causes severe gastrointestinal disease in neonatal piglets, often leading to high mortality. To better understand PEDV pathogenesis, we developed porcine intestinal apical-out organoids derived from the duodenum, jejunum, and ileum that support viral replication and enable long-term experimental manipulation. In this study, we investigated the region-specific responses of these organoids to PEDV infection, focusing on regional characteristics, gene expression, and susceptibility to infection. PEDV replicated efficiently in the apical-out organoids, with significantly higher viral loads in jejunal and ileal organoids than duodenal organoids, indicating region-specific susceptibility. Bulk RNA sequencing and a differential gene expression analysis revealed unique transcriptomic responses across regions. The jejunal and ileal organoids exhibited stronger activation of pathways related to cellular processes, immune regulation, and antiviral defense than the duodenal organoids. Notably, viral entry receptor genes such as <i>ANPEP</i>, <i>ACE2</i>, and <i>DPP4</i> were expressed at higher levels in jejunal and ileal organoids under uninfected conditions, suggesting an innate predisposition for viral entry in these regions. Further analysis identified key upregulated genes involved in immune modulation, inflammation regulation, and tissue integrity, such as <i>SLIT2</i>, <i>MMD2</i>, and <i>PKHD1</i>, along with downregulated genes, including <i>IL-1A</i>, <i>MMP13</i>, and <i>GNA15</i>, that help control inflammation and minimize tissue damage. In conclusion, PEDV infection in porcine intestinal organoids elicits region-specific responses, with increased susceptibility and antiviral activation in jejunal and ileal organoids driven by the differential expression of viral entry receptors and immune-regulatory genes.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 1","pages":"258-272"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12902409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146201647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic and metataxonomic changes in lactating holstein dairy cows during the transition from heat stress to the recovery period.","authors":"Raniel Agustin Valencia, Seon Ho Kim, Janine Isidro Berdos, Myung Hoo Kim, Sung Sill Lee, Sang Suk Lee","doi":"10.5187/jast.2024.e97","DOIUrl":"10.5187/jast.2024.e97","url":null,"abstract":"<p><p>This study investigated the changes in rumen fermentation characteristics, blood parameters, and rumen microbial communities of Holstein dairy cows in the early lactation stage during heat stress conditions and subsequent recovery. This study aimed to fill the significant knowledge gaps regarding the recovery of dairy cattle from heat stress during the early stages of lactation. Metataxonomic analysis was used to identify potential biomarkers and metabolites associated with metabolic disease prediction. The temperature-humidity index was recorded on a dairy farm to define the heat stress and recovery periods. Using the Bray-Curtis dissimilarity index, principal coordinate analysis revealed that both the heat stress and recovery periods affected the overall composition of the rumen bacterial community. The first three principal coordinates explained 33.10%, 19.10%, and 12.30% of the total variation, indicating the significant (<i>p</i> < 0.01) influence of temperature changes on the dominance of rumen microbes and the rumen environment. However, alpha diversity measurements were unaffected in both periods. Metataxonomic analysis (average relative abundance 2%) of cows in both periods revealed ten predominant genera: <i>Prevotella</i>, <i>Ruminococcus</i>, <i>Selenomonas</i>, <i>Gilliamella</i>, <i>Duncaniella</i>, <i>Succiniclasticum</i>, <i>Paraprevotella</i>, <i>Bacteriodes</i>, <i>Lentimicrobium</i>, and <i>Treponema</i>. During heat stress, significant alterations were observed in the levels of three organic acids, six fatty acids, and thirteen amino acids. Furthermore, heat stress caused a significant increase in blood serum HSP27 and HSP70 levels (both <i>p</i> < 0.01), whereas blood serum glucose (<i>p</i> = 0.001) and blood urea nitrogen (<i>p</i> < 0.001) decreased. Heat stress significantly increased blood serum ketone concentrations (<i>p</i> = 0.005), tended to decrease cholesterol levels (<i>p</i> = 0.053), reduced blood urea nitrogen concentrations (<i>p</i> < 0.001), and affected total protein (<i>p</i> = 0.002), aspartate aminotransferase (<i>p</i> = 0.049), and total bilirubin concentrations (<i>p</i> = 0.010). The levels of blood serum minerals, such as calcium, phosphorus, and magnesium, as well as ruminal pH, ammonia-N, acetate, and butyrate, were not affected during either period. Heat stress influenced propionate (<i>p</i> = 0.006) and total volatile fatty acids (<i>p</i> = 0.030). Overall, heat stress during early lactation resulted in significant shifts within the rumen bacterial community structure, accompanied by corresponding changes in blood metabolite profiles.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 1","pages":"324-356"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12902393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146201632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Far-infrared ray and β-mannanase influence on growth performance, meat quality, gut morphology, and blood parameters in nutritionally challenged broilers.","authors":"Jeseok Lee, Sehyeok Oh, Elijah Ogola Oketch, Shan Randima Nawarathne, Nuwan Chamara Chathuranga, Haeeun Park, Taichang Hur, Myunghwan Yu, Jung Min Heo","doi":"10.5187/jast.2500273","DOIUrl":"10.5187/jast.2500273","url":null,"abstract":"<p><p>This study investigated the effects of far-infrared ray (FIR) radiation on broiler performance, carcass traits, meat quality, intestinal morphology, and blood metabolites in birds fed different energy and amino acid (EAA) levels with or without β-mannanase from hatch to day 35. A total of 384 one-day-old Ross 308 broilers were assigned to a 2 × 2 × 2 factorial design: FIR radiation (yes or no), dietary EAA level (normal or low), and β-mannanase supplementation (yes or no). Data were analyzed using three-way ANOVA to evaluate the main and interactive effects of these factors, with Tukey's test applied for multiple comparisons. FIR exposure improved (<i>p</i> < 0.001) average daily gain (ADG) and average daily feed intake (ADFI) on days 1-35. Feed conversion ratio (FCR) was reduced (<i>p</i> < 0.05) on days 1-10, 11-21, and overall. FIR combined with β-mannanase enhanced (<i>p</i> < 0.001) ADG on days 1-10 and 11-21. Broilers fed a low EAA diet and exposed to FIR had higher (<i>p</i> < 0.01) ADFI on days 1-10. On day 35, FIR increased (<i>p</i> < 0.001) moisture, crude protein, and ash content in breast meat and improved (<i>p</i> < 0.05) relative breast meat weight. FIR also enhanced (<i>p</i> < 0.01) water-holding capacity and pH, while reducing (<i>p</i> < 0.05) cooking loss. FIR improved intestinal morphology by increasing (<i>p</i> < 0.001) villus height on days 21 and 35. A significant three-way interaction (<i>p</i> < 0.01) among FIR, EAA level, and β-mannanase was observed for villus height on day 21. Immunologically, FIR reduced (<i>p</i> < 0.01) blood levels of IL-1β and TNF-α on days 21 and 35, and IFN-γ on day 35. FIR also lowered (<i>p</i> < 0.05) blood glucose and lactate levels on both days. In conclusion, FIR radiation, particularly under nutrient-challenged conditions, enhanced broiler growth performance, carcass yield, meat quality, and immune status, with β-mannanase providing additional early growth benefits. FIR technology shows potential as a non-invasive, feed-independent strategy to improve commercial broiler productivity, warranting further field evaluation.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 1","pages":"197-218"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12902473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146201828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of phytobiotics on intestinal barrier function and gut microbiome in weaned piglets challenged with enterotoxigenic <i>Escherichia coli</i>.","authors":"Gi Beom Keum, Hyunok Doo, Jinok Kwak, Xingmin Sun, Jinho Cho, Hyeun Bum Kim","doi":"10.5187/jast.2500128","DOIUrl":"10.5187/jast.2500128","url":null,"abstract":"<p><p>Weaned piglets are highly susceptible to infections caused by enterotoxigenic <i>Escherichia coli</i> (ETEC), and phytobiotic supplementation has been explored as a potential strategy to prevent or mitigate such infections during the weaning period. However, comprehensive studies on the specific effects of phytobiotics on pig gut health and microbiota composition remains limited. Therefore, this study aimed to investigate the effects of various phytogenic feed additives (PFAs) on intestinal barrier function and gut microbiota composition in weaned piglets challenged with ETEC. A total of 63 weaned piglets ([Yorkshire × Landrace] × Duroc), 28 days old with an initial body weight of 8.03 ± 0.43 kg, were used in a 21-day trial. Piglets were randomly assigned to one of seven treatment groups: NC, negative control (basal diets without <i>E. coli</i> challenge); PC, positive control (basal diets + <i>E. coli</i> challenge); T1, PC + 0.04 % P1; T2, PC + 0.01 % P2; T3, PC + 0.10 % P3; T4, PC + 0.04 % P4; T5, PC + 0.10 % P5. The five phytobiotic materials were as follows: a bitter citrus extract rich in flavonoids (P1), a microencapsulated blend of thymol and carvacrol (P2), a composite of P1 and P2 (P3), a grape polyphenol-based mixture containing green tea and hops (P4), and a fenugreek seed powder rich in saponins (P5). Piglets in challenged groups were orally inoculated with ETEC at a concentration of 1.2 × 10¹⁰ CFU/mL from days 8 to 10. The results showed that PFA supplementation upregulated the expression of tight junction and mucin-related genes including ZO-1 and MUC3. Based on 16S rRNA gene sequencing, distinct microbial shifts were observed depending on the specific PFA composition. The relative abundances of beneficial genera such as <i>Lactobacillus</i>, <i>Olsenella</i>, and <i>Collinsella</i> increased following PFA supplementation. These genera are known to be associated with short-chain fatty acid production, improved gut health, and increased resistance to pathogens. Additionally, increases in <i>Acinetobacter</i> and <i>Medioterraneibacter</i>, which may be involved in the metabolism of plant-derived compounds, were observed. Collectively, our results suggest that PFAs may enhance gut health and contribute to the stabilization of the intestinal microbiota in weaned piglets.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 1","pages":"181-196"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12902666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146201866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of solar radiation on spermatozoa abnormality and motility in bulls.","authors":"Onur Şahin","doi":"10.5187/jast.2024.e118","DOIUrl":"10.5187/jast.2024.e118","url":null,"abstract":"<p><p>The aim of this study was to investigate the effects of solar radiation on spermatozoa motility and abnormalities. The material of the study consisted of 28 bulls of the Holstein Friesian, Brown Swiss and Simmental breeds reared in a private AI center for artificial insemination, as well as the data of 1,539 collected ejaculations of these bulls and the meteorological data of the research area. The SPPS 25.0 program was used for statistical analysis. The differences between the solar radiation intensity groups for the concentration and proportion of distal midpiece reflex (DMR) and coiled tail (CT) spermatozoa were highly statistically significant (<i>p</i> < 0.01) and the differences found for the concentration and proportion of proximal drop (PD) spermatozoa were statistically significant (<i>p</i> < 0.05). In contrast, the differences found between the groups for CT spermatozoa were statistically insignificant (<i>p</i> > 0.05). The difference between the groups of solar radiation for the ratio of solve spermatozoa (SL) to abnormal spermatozoa was statistically significant (<i>p</i> < 0.01), while the differences for Static spermatozoa (ST), Progressive spermatozoa (PR), and motile spermatozoa (MO) spermatozoa ratios were statistically insignificant (<i>p</i> > 0.05). The differences observed between the temperature-humidity index groups in all abnormal spermatozoa ratios were not statistically significant (<i>p</i> > 0.05). The intensity of solar radiation was positively and significantly (<i>p</i> < 0.05) associated with ST spermatozoa, while it was negatively and significantly (<i>p</i> < 0.01) associated with MO and SL spermatozoa. There was a positive and significant (<i>p</i> < 0.01) association between solar radiation intensity and abnormal spermatozoa bent tail (BT), DMR, distal drop (DD) and proximal drop (PD), while CT showed a negative and significant (<i>p</i> < 0.05) association with spermatozoa. There was a positive and significant relationship between temperature-humidity index (THI) and ST motility traits, a negative and significant relationship with MO spermatozoa and a negative and significant relationship with SL spermatozoa ratio. There was a positive and significant relationship between THI and BT, DMR, DD and PD abnormal spermatozoa, while a negative and significant relationship was found with CT.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 1","pages":"105-125"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12902411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146201623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of irradiation on microbiological safety and physicochemical properties of dry pet food.","authors":"Dongbin Park, Anand Kumar Sethukali, Minwoo Choi, Jae-Kyung Kim, Hyun Jung Lee, Cheorun Jo","doi":"10.5187/jast.2024.e117","DOIUrl":"10.5187/jast.2024.e117","url":null,"abstract":"<p><p>The objective of this study was to investigate the effects of electron beam (EB) and X-ray (XR) irradiation on dry pet food during long-term storage. The samples were irradiated with EB and XR at doses of 0, 2.5, 5, 10, and 20 kGy, and their microbial safety and quality/oxidation properties were analyzed over 56 days under storage conditions of 25°C and 70% relative humidity. As a result, total aerobic bacteria and yeasts and molds significantly decreased as the doses of EB and XR increased. When treated with 10 kGy for both irradiations, no bacteria were detected in the dry pet food, and this effect remained during the 56-day storage period. While EB and XR were effective in reducing aflatoxin B1 in solution, they showed limited effect on dry pet food. On the other hand, changes in quality traits such as proximate compositions, pH, water activity, color, and volatile basic nitrogen due to EB and XR were negligible. However, both types of irradiation induced lipid and protein oxidation in dry pet food. Also, a significant increase was observed in oxidation-related volatile compounds such as hydrocarbons, aldehydes, and ketones with EB and XR treatment, which suggested these changes could potentially impact the flavor of the dry pet food. The current findings confirm the efficient microbial reduction of dry pet food by EB and XR and the consequent changes in quality and oxidative properties. Future research should focus on sensory evaluations to understand the implications of these oxidized substances on pet preferences and explore potential methods to mitigate negative effects.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 1","pages":"306-323"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12902436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146201830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete genome sequence of bacteriocin-producing <i>Lactococcus lactis</i> subsp. <i>lactis</i> DOME 6301 with potential oral-pathogen control applications.","authors":"Arxel G Elnar, Yujin Jang, Byeong-Gwan Eum, Yookyung Hur, Chul Sung Huh, Geun-Bae Kim","doi":"10.5187/jast.2024.e62","DOIUrl":"10.5187/jast.2024.e62","url":null,"abstract":"<p><p><i>Lactococcus lactis</i> subsp. <i>lactis</i> DOME 6301, isolated from bovine milk, produces an antimicrobial compound that inhibits oral pathogens including <i>Streptococcus mutans</i>, <i>Prevotella intermedia</i>, and <i>Fusobacterium nucleatum</i>. The entire genome of <i>L. lactis</i> DOME 6301 was sequenced and assembled <i>de novo</i> using the PacBio RS II platform. The genome was 2,532,858 bp in length, assembled into three contigs, and had a guanine and cytosine (G + C) ratio of 35%. The annotation results revealed 2,469 protein-coding sequences, 22 rRNA genes, and 78 tRNA genes. Genes involved in the utilization of complex carbohydrates (i.e., cellulose, xylose, pullulan, amylose, maltodextrin, and arabinofuranose) and synthesis of the bacteriocin, nisin Z, were detected. In addition, genes encoding antimicrobial resistance and virulence factors (i.e., hemolysin and enterotoxin) were detected. Whole-genome analysis of strain DOME 6301 contributed to our understanding of the evolution of the taxa and provided the basis for the correct selection of probiotic candidates for downstream applications.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 1","pages":"366-370"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12902663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146201858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the correlation of glucose-regulated protein 78 with sperm motility and kinematic parameters insights into male fertility.","authors":"Claudine Uwamahoro, Jae-Hwan Jo, Seung-Ik Jang, Eun-Ju Jung, Woo-Jin Lee, Jeong-Won Bae, Dae-Hyun Kim, Jun Koo Yi, Dong Yep Oh, Seung Won Nam, Eu Jin Chung, Jae Jung Ha, Woo-Sung Kwon","doi":"10.5187/jast.2024.e108","DOIUrl":"10.5187/jast.2024.e108","url":null,"abstract":"<p><p>Glucose-regulated protein 78 (GRP78), which belongs to the heat shock protein 70 family, is a chaperone essential for maintaining cellular balance in the endoplasmic reticulum. It acts as a receptor on the cell surface, affecting cell growth and survival. Recent research has emphasized the involvement of GRP78 in protein folding, endoplasmic reticulum stress signaling, and cancer cell proliferation and highlighted its presence on sperm cells, where it contributes to spermatogenesis and sperm capacitation. Despite these findings, the association of GRP78 with sperm motility and motion kinematic parameters remains insufficiently understood. This study assessed the correlation between GRP78 and various sperm motility and kinematic parameters, which are essential for the capacitation process. GRP78 expression was positively correlated with linearity and straightness and negatively correlated with total sperm motility and beat cross frequency. However, GRP78 expression did not exhibit a significant correlation with progressive sperm motility, average path velocity, curvilinear velocity, straight-line velocity, and amplitude of lateral head displacement. These results provide novel insights into the correlation between GRP78 and sperm motility, as well as its potential implications for male fertility.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 1","pages":"96-104"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12902372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146201603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}