Journal of Animal Science and Technology最新文献

{"title":"Nutritional and physiological response to feed form and sodium bicarbonate-adjusted dietary electrolyte balance in lactating sows under heat stress.","authors":"Kwang Mun Kim, Jun Young Mun, Habeeb Tajudeen, Abdolreza Hosseindoust, Sang Hun Ha, So Dam Choi, Seon Ah Park, Sang Sik Lee, Yong-Soo Park, Eun-Ji Song, Young-Do Nam, Jin Soo Kim","doi":"10.5187/jast.2500270","DOIUrl":"https://doi.org/10.5187/jast.2500270","url":null,"abstract":"<p><p>Heat stress (HS) during lactation poses a critical challenge in swine production, often impairing feed intake, metabolic function, and reproductive performance in sows. This study aimed to evaluate the effects of feed form (mash vs. pellet) and dietary electrolyte balance (dEB; 230 vs. 290 mEq/kg) adjusted with sodium bicarbonate on sow performance, litter growth, immune response, antioxidant status, and gut microbiota under HS conditions. A total of 40 multiparous sows were assigned to four treatments in a 2 × 2 factorial design: Mlow (mash + 230 mEq/kg), Mhigh (mash + 290 mEq/kg), Plow (pellet + 230 mEq/kg), and Phigh (pellet + 290 mEq/kg). Each treatment contained 10 sows with 3-5 parity and their initial body weight was 241.83 ± 16 kg at d 112 of lactation. The trial spanned from parturition to weaning (21 days) during summer at an average temperature of 28.8°C. Results showed that sows fed pelleted diets (Plow, Phigh) and higher dEB levels (Mhigh, Phigh) had higher (<i>p</i> < 0.001) average daily feed intake. Piglets from Mhigh and Phigh sows had increased (<i>p</i> = 0.001) weaning weights. Dry matter digestibility was increased (<i>p</i> = 0.022) in sows receiving pelleted diets. The tumour necrosis factor-alpha was lower (<i>p</i> < 0.001) in Plow, Phigh, and Mhigh (<i>p</i> = 0.010), with an interaction (<i>p</i> = 0.013) in feed forms and dEB. The interleukin-1β was lower (<i>p</i> < 0.001) in Plow and Phigh, with higher (<i>p</i> < 0.001) superoxide dismutase activity in Phigh and Plow. Hair cortisol was lower (<i>p</i> = 0.048) in pelleted groups, suggesting lower physiological stress. Although alpha diversity did not differ, beta diversity and relative abundance of <i>Lactobacillus</i> and <i>Turicibacter</i> indicated microbial shifts influenced by feed form. In conclusion, pelleted feed form and higher dEB, particularly the Phigh treatment, enhanced feed intake, litter performance, and anti-inflammatory status without negatively affecting milk composition or gut integrity. These findings support the integration of feed form and electrolyte strategies to improve sow productivity under HS conditions.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 5","pages":"1460-1476"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13678364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional strategies using synbiotics and organic acids to enhance piglet post-weaning growth and gut health.","authors":"Su Hyup Lee, Sunbong Choi, Young Bin Park, Hyun-Jun Jang, Soyeon Park, Yangseon Kim, Jin-Ki Park, Sung Ho Lee, Dong Wook Kim","doi":"10.5187/jast.2500456","DOIUrl":"https://doi.org/10.5187/jast.2500456","url":null,"abstract":"<p><p>This study aimed to evaluate the effects of dietary supplementation with probiotics (PRO), synbiotics (SYN), and SYN + benzoic acid (SYP) on growth performance, stress response, nutrient digestibility, blood parameters, immune status, and intestinal health of weanling pigs. Four hundred weaned pigs (Landrace × Yorkshire × Duroc) were allocated to four treatment groups: control (basal diet), PRO (<i>Bacillus licheniformis</i>), SYN (<i>Bacillus licheniformis</i> + dietary fiber), and SYP. The feeding trial lasted for 28 days. Notably, pigs in the SYN and SYP groups showed greater average daily gain during phase 1 (<i>p</i> = 0.038), phase 2 (<i>p</i> = 0.008), and the overall period (<i>p</i> = 0.011) compared with those in the control group. Gain-to-feed ratio was also improved during phase 2 (<i>p</i> = 0.003) and the overall experimental period (<i>p</i> = 0.012). The SYP group demonstrated significantly improved dry matter digestibility during phase 2 (<i>p</i> < 0.05). Serum IL-1β concentrations tended to decrease in pigs fed SYN and SYP diets. In addition, SYN and SYP supplementation increased the concentration of the tight junction protein zona occludens-1 (ZO-1) in the jejunum (<i>p</i> = 0.022). Histological analysis showed greater villus height in the duodenum (<i>p</i> = 0.013) and jejunum (<i>p</i> = 0.038) of pigs fed SYN and SYP diets. Overall, dietary supplementation with synbiotics, particularly in combination with benzoic acid, improved growth performance and intestinal health in weanling pigs.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 5","pages":"1501-1516"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13678349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of scaffold properties for cell-cultured food based on protein sources and their mixtures.","authors":"Seon-Min Oh, Jae Hoon Lee, Tae-Kyung Kim, Jinryong Park, Min-Cheol Kang, Min Kyung Park, Yun-Sang Choi","doi":"10.5187/jast.2500143","DOIUrl":"https://doi.org/10.5187/jast.2500143","url":null,"abstract":"<p><p>For cultured meat applications, the development of protein-based scaffolds is essential to produce sustainable edible materials with suitable textures and functionalities. In this study, scaffolds were fabricated using various plant-based protein sources, including soybean (gel-based soybean protein scaffold, GSP), pea (gel-based pea protein scaffold, GPP), and faba bean (gel-based faba bean protein scaffold, GFP), and mixed protein formulations (S1-S4), and their physicochemical, mechanical, and biological properties were evaluated. All the scaffolds exhibited a pale yellow color and porous surface morphology. Water absorption analysis revealed that GSP exhibited the highest uptake among the single-protein scaffolds. Notably, the partial substitution of pea or faba bean proteins with other plant proteins, such as soy, significantly improved the water absorption capacity compared to that of GPP and GFP. The degradation rate of plant protein-based scaffolds remained below 10% during the early incubation stages, but increased markedly after 12 h. Mixed-protein scaffolds exhibited over 20% degradation at 48 h, whereas single-protein scaffolds showed less degradation. Texture profile analysis demonstrated that mixed-protein scaffolds had significantly higher hardness and chewiness than single-protein scaffolds, likely due to enhanced protein-protein interactions and network formation. However, cell proliferation analysis indicated that single-protein scaffolds supported better cell attachment and proliferation, with scaffolds prepared using faba bean proteins showing the highest proliferation rate. These results suggest that plant-based protein scaffolds can be tailored based on protein composition to optimize both their physicochemical and biological properties, thereby offering promising strategies for the development of edible scaffolds for cultured meat production.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 5","pages":"1586-1598"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13678344/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing canine intestinal health: evodiamine-enriched functional diet modulates microbiota and metabolic pathways.","authors":"Qingzheng Wang, Fuqing Huang, Xu Cheng, Song Tian, Juan Wan, Yun Chen, Xiaojie Xiao, Xin Zhang, Manli Hu","doi":"10.5187/jast.2024.e89","DOIUrl":"https://doi.org/10.5187/jast.2024.e89","url":null,"abstract":"<p><p>This study investigated the effects of an evodiamine (EVO)-enriched functional diet on canine intestinal health, with a focus on its modulation of microbiota composition and promotion of intestinal well-being. After a one-week acclimatization period, dogs were randomly assigned to either the control group or the EVO group, receiving their respective diets for 8 weeks. Physiological and hematological parameters were assessed at 0, 4, and 8 weeks, and fresh feces were collected at 4 and 8 weeks for microbial analysis to evaluate the impact of the functional diet on canine intestinal microorganisms. The 8-week trial revealed that the functional gut diet regulated microbial composition, improving intestinal health without affecting body metrics or routine blood indicators, and the diet's safety was affirmed by normal biochemical indices. Comparative analysis indicated altered microbial abundance in model dogs, highlighting positive changes favoring intestinal barrier enhancement. Alpha diversity analysis confirmed increased species diversity in the EVO group, reflecting a healthier gut. Moreover, the study demonstrated the functional diet's regulatory impact on microbial metabolic pathways and species differences without observed side effects, reinforcing its positive influence on gut health. Therefore, the canine intestinal functional diet containing EVO showed no significant impact on physiological health but exhibited regulatory effects on intestinal microorganisms. EVO effectively modulated microbial abundance and diversity, fostering intestinal mucosal repair and barrier protection. Additionally, the diet improved microbial function by regulating canine intestinal metabolic pathways. This study serves as a valuable reference for future research in promoting canine intestinal health.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 5","pages":"1528-1543"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13678316/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between PI3K/PDK1/AKT pathway-related protein levels and sperm motility in Duroc Boars.","authors":"Seung-Ik Jang, Jae-Hwan Jo, Claudine Uwamahoro, Eun-Ju Jung, Woo-Jin Lee, Jeong-Won Bae, Chanwool Park, Junkoo Yi, Daehyun Kim, Joonho Moon, Dong Yep Oh, Jae Jung Ha, Woo-Sung Kwon","doi":"10.5187/jast.2500234","DOIUrl":"https://doi.org/10.5187/jast.2500234","url":null,"abstract":"<p><p>Male infertility is an increasing global health concern, with reduced sperm motility being a major contributing factor. Among various molecular mechanisms, the phosphoinositide 3-kinase (PI3K)/phosphoinositide-dependent protein kinase-1 (PDK1)/protein kinase B (AKT) signaling pathway has been identified as a key regulator of sperm function, particularly motility. However, the relationship between this pathway and sperm motility is not fully understood. In this study, we investigated the correlation between sperm motility and the levels of PI3K/PDK1/AKT pathway-related proteins in Duroc boar spermatozoa. Sperm motility was assessed using computer-assisted sperm analysis (CASA), and protein levels were measured via enzyme-linked immunosorbent assay (ELISA). Our results revealed significant correlations between signaling components and specific motility parameters. Notably, PI3K levels were negatively correlated with beat cross frequency (BCF), suggesting that excessive activation may impair flagellar motion. In contrast, phosphorylated PDK1 (p-PDK1) and AKT phosphorylated at Thr308 and Ser473 were positively correlated with progressive motility (PRG), supporting a role for AKT activation in enhancing forward movement. Interestingly, both PTEN and phosphorylated PTEN (p-PTEN) showed positive correlations with various velocity parameters, indicating a potential regulatory role in modulating AKT activity to maintain optimal motility patterns. While these findings enhance our understanding of sperm motility regulation, limitations include reliance on the correlation analysis and the absence of direct enzymatic activity measurements. Additionally, these results are specific to Duroc boar spermatozoa and may not be directly applicable to other species. Nonetheless, this study provides foundational insights into the molecular mechanisms underlying sperm motility and underscores the importance of the PI3K/PDK1/AKT pathway in male fertility.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 5","pages":"1371-1381"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13678331/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of pork bones by means of shelf-life property, nutritional composition, and their gelatin's bioactivity.","authors":"Van Ba Hoa, Won-Seo Park, Jun-Sang Ham, In-Seon Bae","doi":"10.5187/jast.2500309","DOIUrl":"https://doi.org/10.5187/jast.2500309","url":null,"abstract":"<p><p>The millions of tons of pork bones produced annually by the meat industry are widely used for human consumption and applied in other industries. This study aimed to evaluate the shelf-life and nutritional composition of pork bones and to develop an efficient method for extracting their bioactive gelatin. Pork bone samples collected at 24 h after slaughter were aerobically packaged and stored at 4°C for 21 days, then evaluated for total aerobic bacteria, discoloration, and fat and protein oxidation. Nutritional composition (fatty acids, amino acids, and collagen contents) was also analyzed. For gelatin extraction, the bone samples were swollen with 0.3% vinegar (4°C for 24 h) and hydrolyzed with 2.0% (v/w) crude ginger or kiwifruit extract for 5 h at 55°C. Results showed that pork bones lost approximately 43.69%-63.76% of their red color values and had high levels of lipid oxidation only after 14 days of storage. The total collagen, essential amino acid, and polyunsaturated fatty acid contents in the bones were 6-11 g/100 g, 403-554 mg/100 g, and 8%-10%, respectively. The hydrolysis with crude enzyme extracts yielded gelatins with multiple molecular weights, mainly in the range of 20-15 kDa. At a 0.7 mg/mL concentration, gelatin samples had DPPH free radical scavenging and ferrous chelating activity of 76%-89% and 67%-81%, respectively. Pork bone is a nutrient-rich by-product, and using vinegar and plant-derived enzyme extracts could be an effective and safe alternative for extracting bioactive compounds from pork bones.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 5","pages":"1599-1616"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13678348/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Histamine-N-methyltransferase-like (HNMT-like) gene polymorphisms on the anserine and carnosine content in chicken breast meat.","authors":"Jean Pierre Munyaneza, Minjun Kim, Eunjin Cho, Aera Jang, Cheorun Jo, Ki-Chang Nam, Hyo Jun Choo, Jun Heon Lee","doi":"10.5187/jast.2400225","DOIUrl":"https://doi.org/10.5187/jast.2400225","url":null,"abstract":"<p><p>Anserine is an abundant peptide in chicken meat and influences the meat flavor. The content of anserine in meat as other flavor precursors is influenced by many genes as well as environmental factors. This study aimed to identify the single nucleotide polymorphisms (SNPs) in the <i>HNMT-like</i> gene and assess their possible associations with the content of anserine in the red-brown Korean native chicken (KNC-R). We used three hundred and eighty-four chickens (males, n = 192; females, n = 192) aged 10 weeks old to genotype three synonymous SNPs (rs317607580C>T, rs316762204A>G, and rs316857074A>G) in <i>HNMT-like</i> gene by using PACE genotyping technology. We used a two-way ANOVA of the R program to test the effects of the <i>HNMT-like</i> genotypes on the content of anserine in KNC-R. We found a significant association (<i>p</i> < 0.05) between the genotypes of the synonymous SNP: rs316762204A>G and the content of the anserine in KNC-R. The synonymous SNP: rs316762204A>G of the <i>HNMT-like</i> gene might be used as a genetic marker in the selection and breeding of chickens with desired flavored meat.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 5","pages":"1362-1370"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13678358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploratory assessment of N-carbamylglutamate supplementation on feed efficiency and protein utilization in early growing pigs fed low crude protein diet.","authors":"So Dam Choi, Jun Young Mun, Abdolreza Hosseindoust, Habeeb Tajudeen, Seon Ah Park, Priscilla Neves Silvestre, Sang Sik Lee, Jin Soo Kim","doi":"10.5187/jast.2500167","DOIUrl":"https://doi.org/10.5187/jast.2500167","url":null,"abstract":"<p><p>Reducing dietary crude protein (CP) levels while supplementing with essential amino acids (AA) has been widely studied as a strategy to maintain nutrient balance while lowering nitrogen excretion. Among feed additives, N-carbamylglutamate (NCG), an arginine precursor with superior stability and mitochondrial permeability, has emerged as a promising feed additive for improving intestinal development and overall pig performance. A total of 240 crossbred grower pigs were allotted to four dietary treatments based on initial body weight. Treatments were arranged in a 2 × 2 factorial design with two levels of dietary CP (MCP, 16% moderate CP; LCP, 14% low CP) and two levels of NCG supplementation (without NCG [WN]; NS, 0.1% NCG supplementation). Each treatment had 10 replicates, with six (3 male and 3 female) pigs per pen, and the feeding trial lasted for 4 weeks. Pigs fed the MCP diet tended to have higher final BW (<i>p</i> = 0.088) compared with the LCP diet. Feed efficiency was greater in MCP than LCP (<i>p</i> < 0.05) and also tended to be enhanced in the NS (<i>p</i> = 0.089). CP digestibility tended to increase in the NS group compared with the WN (<i>p</i> = 0.093). The digestibility of arginine and tyrosine was increased in the NS group compared with the WN group (<i>p</i> < 0.05). Furthermore, lysine (<i>p</i> = 0.067), glycine (<i>p</i> = 0.058), and the average of essential AA (<i>p</i> = 0.091) digestibility tended to be increased in the NS. The NS group showed higher concentrations of arginine, lysine, essential AA, non-essential AA, and total AA in the blood (<i>p</i> < 0.05). Trypsin activity showed a tendency to increase in the NS group compared with the WN group (<i>p</i> = 0.087). In conclusion, dietary NCG supplementation in a low-protein diet showed potential to improve feed efficiency, AA digestibility, and serum AA profiles in early growing pigs.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 5","pages":"1416-1427"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13678308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-time determination of swine manure liquid compost maturity by oxidation-reduction potential and pH monitoring.","authors":"Seung Soo Kim, Soo Min Shim, Seung Gun Won, Chang Six Ra","doi":"10.5187/jast.2500220","DOIUrl":"https://doi.org/10.5187/jast.2500220","url":null,"abstract":"<p><p>The aim of this study was to develop a real-time diagnostic method for assessing the maturity of liquid compost from swine manure by monitoring the oxidation-reduction potential (ORP) and pH, two key indicators of the oxidative status of organic matter and nitrogen compounds. The experimental system comprised a cylindrical bioreactor equipped with an aeration and mixing device. During the liquid composting process of swine manure in the bioreactor, the ORP and pH were continuously monitored, while changes in the liquid compost maturity and concentrations of soluble nitrogen and organic matter were observed. In addition, the physicochemical differences between mature liquid compost (MLC) and non-mature liquid compost (NMLC) were comparatively analyzed. The MLC exhibited significantly higher ORP and lower pH with lower ammonium nitrogen concentrations and higher concentrations of oxidized nitrogen compounds (NOₓ-N) compared to NMLC. A diagnostic method combining ORP and pH thresholds was proposed, where samples with an ORP greater than or equal to 180 mV were classified as MLC. In addition, samples with an ORP between 0 and 180 mV and pH < 8 were classified as MLC. Field validation at a swine manure liquid composting facility confirmed the accuracy of the diagnostic method, achieving 100% agreement with liquid compost maturity analysis using an automatic liquid compost maturity tester. This study provides a simple and effective tool for optimizing liquid compost production, minimizing ammonia emissions, and enhancing liquid compost maturity in swine manure recycling systems.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 5","pages":"1645-1661"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13678305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-analysis with batch-effect adjustment reveals breed-specific ruminal microbiome variation of beef cattle and its implications for meat quality and productivity.","authors":"Ryukseok Kang, Chaemin Yu, Yeonsue Gwon, Sangmin Lee, Joohyuk Yang, Jongsik Jeong, Geonwoo Kim, Woohyung Lee, Wanho Jo, Myeong-Gwan Oh, Changbeen Jang, Mekonnen Tilahun, Joonpyo Oh, Tansol Park","doi":"10.5187/jast.2500254","DOIUrl":"https://doi.org/10.5187/jast.2500254","url":null,"abstract":"<p><p>Various beef cattle breeds are raised in different countries. Hanwoo and Wagyu breeds are primarily raised in Korea and Japan, respectively, whereas Angus breed is one of the most widely bred beef cattle breed worldwide. Although microbiome studies have been conducted for each breed, comparative analyses of the ruminal microbiome and their functions across breeds remain limited. Moreover, the potential links between the ruminal microbiome and animal traits related to meat quality and productivity have not been studied. Based on 13 studies comprising 954 samples (Hanwoo: 384, Angus: 246, Wagyu: 324) with the application of an optimal batch-effect adjustment tool, this meta-analysis aimed to compare the ruminal microbiome across Hanwoo, Angus, and Wagyu and explore potential associations between breed-specific microbiome profiles and key phenotypic traits, such as meat quality and production performance. The overall microbial composition at the phylum, genus, and Kyoto Encyclopedia of Genes and Genomes (KEGG) ortholog levels was effectively adjusted batch-effect using the selected adjustment tool (ConQuR). Subsequent analyses were conducted using batch-effect adjusted microbiome data to investigate the breed-specific differences. Cellulolytic bacteria such as <i>Bacteroides</i>, <i>Fibrobacter</i>, <i>Lacrimispora</i>, and <i>Ruminococcus</i> were dominant in Angus, whereas saccharolytic bacteria such as <i>Selenomonas</i>, <i>Olsenella</i>, <i>Sporomusa</i>, <i>Streptococcus</i>, and <i>Bifidobacterium</i> were relatively abundant in Wagyu. Predicted KEGG modules revealed that ubiquinone and biotin biosynthesis pathways were enriched in Angus, whereas amino acid biosynthesis was enriched in Wagyu. Hanwoo exhibited intermediate traits at both the phylum and genus taxonomic levels. In the comparative network analysis, <i>Prevotella</i> and <i>Dialister</i> were the keystone genera in Angus, <i>Mogibacterium</i> in Wagyu, and <i>Streptococcus</i> in Hanwoo. In archaeal microbiome comparisons, methane metabolism-related KEGG modules were enriched in Angus. KEGG modules and taxa previously known as high average daily gain-related were relatively enriched in Angus, whereas Wagyu was characterized by those related to high intramuscular fat. Hanwoo exhibited intermediate traits in both productivity and meat quality, and the microbiome features were between those of Angus and Wagyu. These findings suggest a potential link between the ruminal microbiome and meat quality- and productivity-related traits in beef cattle.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 5","pages":"1544-1566"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13678367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}