Journal of Animal Science and Technology最新文献

{"title":"Dietary supplementation of <i>Lactobacillus salivarius</i> in suckling and weanling piglets modulates intestinal microbiota, morphology and improves growth performance.","authors":"Elick Kinara, Joseph Moturi, Abdolreza Hosseindoust, Jun Young Mun, Habeeb Tajudeen, Sang Hun Ha, Se Rin Park, Su Hyub Lee, Jin Soo Kim","doi":"10.5187/jast.2024.e58","DOIUrl":"https://doi.org/10.5187/jast.2024.e58","url":null,"abstract":"<p><p>Suckling piglets face the hurdle of pathogenic inversion before the full development of their gastrointestinal tract. The provision of <i>Lactobacillus</i> (<i>L</i>) <i>salivarius</i> guarantees resilient gut health, controls pathogens, increases microbiota, and fortifies intestinal structure. We evaluated the effect of <i>L. salivarius</i> LS144 probiotic given to suckling piglets through the post-weaning stage on the gut microbiota, intestinal morphology, and growth performance. A total of 120 three-day-old crossbred (Landrace × Yorkshire × Duroc) piglets were assigned to four dietary treatments on the basis of initial body weight. The NN group was not supplemented with the probiotic in both the suckling and post-weaning phases, the NP group was supplemented with the probiotic during the post-weaning phase, the PN group was supplemented with the probiotic only during the suckling phase, and the PP group was supplemented with the probiotic during both the suckling and post-weaning periods. Results revealed that the average daily gain was higher (<i>p</i> < 0.05) in the PN and PP groups than in the NN and NP groups in phase 1. In the overall study (1-51 d), average daily gain was greater (<i>p</i> < 0.05) in the PP treatment compared to all other groups. The average daily feed intake was higher (<i>p</i> < 0.05) in the PP group (22-51 d) than all groups. The villus height was greater in the duodenum (<i>p</i> < 0.05), jejunum (<i>p</i> < 0.05), and ileum (<i>p</i> < 0.05) in the PP compared with the NN. The pH of the intestinal digesta was higher (<i>p</i> < 0.05) in the NN treatment than in the PN and PP treatments in the duodenum. The population of total <i>L</i>. bacteria was greater in both the PN and PP groups compared to the NN treatment in the duodenum (<i>p</i> < 0.01), jejunum (<i>p</i> < 0.05), ileum (<i>p</i> < 0.01), and cecum (<i>p</i> < 0.01). There was no significant difference in the population of total anaerobes, Clostridium, and coliform bacteria in the duodenum, jejunum, ileum, and cecum among the groups. Based on these findings, dietary supplementation with <i>L. salivarius</i> in suckling piglets continued to post-weaning could establish appropriate intestinal microbiota, improve feed intake, and increase the villus height, which translates to improved growth performance during this critical period in piglet's life.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"67 4","pages":"826-838"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12380024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth performance of male broiler chickens in different growth phases in response to amino acid concentrations in the pre-starter diet.","authors":"Su Hyun An, Changsu Kong","doi":"10.5187/jast.2024.e55","DOIUrl":"https://doi.org/10.5187/jast.2024.e55","url":null,"abstract":"<p><p>An experiment involving 720 one-day-old male broilers (Ross 308) was conducted to investigate the effects of graded levels of crude protein and standardized ileal digestible (SID) amino acids (AA) on growth performance during the pre-starter period (0 to 7 d), and to compare the subsequent growth performance of birds fed a commercial diet in the later phase (8 to 28 d). On d 1, all birds were individually weighed and allocated to six groups with eight replicate pens (15 birds/pen). Broilers were fed diets containing six different dietary SID AA levels relative to the 90 to 115% requirement for 7 d. From d 8 to 28, birds were fed a commercial diet containing nutrient levels meeting their dietary requirements. The body weight gain (BWG; <i>p</i> = 0.044) and gain-to-feed ratio (G:F; <i>p</i> = 0.005) of birds increased quadratically, and feed intake of birds linearly increased with increasing dietary AA concentration during d 0 to 7. Following the transition to a commercial diet, body weight at 14, 21, and 28 d, BWG, feed intake, and G:F linearly increased (<i>p</i> < 0.05). From 22 to 28 days of age, BWG (<i>p</i> = 0.001) and feed intake (<i>p</i> = 0.008) of birds linearly increased compared to the 90% SID AA treatment, whereas G:F was not affected (<i>p</i> = 0.088) by dietary treatment. Overall, BWG and the growth rate of broilers aged 8 to 28 d also exhibited linear increments (<i>p</i> < 0.01) by the dietary AA concentrations in diets during the first week. The study findings confirm the influence of dietary AA concentrations on the growth performance of broilers in the first week after hatch, demonstrating that this impact persists in the later growth stage. Therefore, ensuring sufficient dietary AA intake during the first week of life can enhance performance in later stages of development in broiler chickens.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"67 4","pages":"839-852"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12380020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association and fine-mapping analyses identify novel candidate genes affecting serum cortisol levels using imputed whole-genome sequencing data in pigs.","authors":"Tae-Hee Kim, Hyeon-Ah Kim, Jae-Bong Lee, Dongwon Seo, Yuju Lee, Yong Jun Kang, Sang-Geum Kim, Sang-Hyun Han, Seung-Hwan Lee, Cedric Gondro, Hee-Bok Park, In-Cheol Cho","doi":"10.5187/jast.2024.e83","DOIUrl":"https://doi.org/10.5187/jast.2024.e83","url":null,"abstract":"<p><p>In swine breeding programs, it has now become critically important to emphasize selection for resilience to external environmental stress factors that have negatively impacted the productivity of pigs, such as those due to climate change induced temperature increases, or the intensification of housing environments. Secretion of cortisol, a neurophysiological change mediated by the hypothalamic-pituitary-adrenal axis, is a central mechanism in the biological stress response. This hormone is closely related to pig robustness and health and can serve as an informative indicator of stress resistance and robustness in pigs. To identify positional candidate genes and their genetic variants influencing blood cortisol levels, we conducted genome-wide association study (GWAS), joint linkage and linkage disequilibrium (LALD) mapping and Bayesian fine-mapping analysis in an F₂ resource population generated by crossing Duroc pigs with Korean native pigs. The data used in the study included 243 F₂ animals. We utilized imputed whole-genome sequencing data for our analyses. GWAS results revealed a genome-wide significant quantitative trait locus (q-value < 0.05) located within a ~2.46 Mb region between single nucleotide polymorphisms 7:114031215 and 7:116497417 on pig chromosome 7, which accounted for 12.65% of the phenotypic variation. LALD mapping analysis was performed to narrow down the confidence interval (CI) of the quantitative trait locus which resulted in a CI of 2.39 Mb (7:114409266~116803751). Further, to identify candidate causal genes within the 2.39 Mb region, fine-mapping analysis was performed within the region. The fine-mapping analysis identified <i>SERPINA1</i>, <i>ITPK1</i>, <i>CLMN</i>, <i>SERPINA12</i>, and <i>PRIMA1</i>, in addition to <i>SERPINA6</i>, which was previously shown to be associated with blood cortisol levels. Our results identified positional candidate genes and genetic variants associated with serum cortisol concentrations that can be included in marker panels for genomic prediction to improve selection for robustness in pigs.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"67 4","pages":"759-772"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12380018/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to: Impacts of guidelines transition on greenhouse gas inventory in the livestock sector: a study case of Korea.","authors":"Eska Nugrahaeningtyas, Jong-Sik Lee, Dong-Jun Lee, Jung-Kon Kim, Kyu-Hyun Park","doi":"10.5187/jast.2024.e7.e","DOIUrl":"https://doi.org/10.5187/jast.2024.e7.e","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.5187/jast.2024.e7.].</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"67 4","pages":"948"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12380021/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial activity of <i>Pediococcus pentosaceus</i> strains against diarrheal pathogens isolated from pigs and effect on paracellular permeability of HT-29 cells.","authors":"Eunbee Cho, Yoonjeong Yoo, Yohan Yoon","doi":"10.5187/jast.2024.e47","DOIUrl":"https://doi.org/10.5187/jast.2024.e47","url":null,"abstract":"<p><p>This study aimed to investigate lactic acid bacteria with antimicrobial activities against infectious diarrheal pathogens in pigs and their genetic characteristics. Acid-resistant lactic acid bacteria were examined for bile resistance, pancreatic enzyme resistance, gelatinase and urease activities, and antibiotic resistance. Subsequently, selected isolates were examined for antimicrobial activities against <i>Campylobacter coli</i>, <i>Clostridium perfringens</i>, <i>Escherichia coli</i>, and <i>Salmonella</i> Typhimurium, and their effects on paracellular permeability and the expression of tight junction protein-encoding genes in HT-29 cells were assessed. Whole genome sequencing was performed to identify the genes related to safety and antibacterial activity. Of the 51 isolates examined, 12 were resistant to bile and pancreatin and did not produce gelatinase and urease. Of these 12, isolates 19, 20, 30, 36, and 67 showed tetracycline resistance and isolates 15, 19, and 38W showed antimicrobial activity against infectious diarrheal bacteria. Treatment with isolate 38W significantly reduced the paracellular permeability induced by <i>E. coli</i> in HT-29 cells and alleviated the expression of tight junction protein-encoding genes (<i>claudin-1</i>, <i>occludin</i>, and <i>ZO-1</i>) induced by <i>E. coli</i> inoculation. Isolates 15, 19, and 38W were named as <i>Pediococcus pentosaceus</i> SMFM2016-NK1, SMFM2016-YK1, and SMFM2016-WK1, respectively. Bacteriocin-related genes were <i>YheH</i>, <i>ytrF</i>, <i>BceA</i>, <i>BceB</i>, and <i>MccF</i> in SMFM2016-NK1; <i>YheH</i>, <i>ytrF</i>, <i>BceA</i>, <i>BceB</i>, <i>entK</i>, <i>lcnA</i>, <i>MccF</i>, and <i>skgD</i> in SMFM2016-YK1; and <i>YheH</i>, <i>ytrF</i>, <i>BceA</i>, <i>BceB</i>, and <i>MccF</i> in SMFM2016-WK1. SMFM2016-YK1 harbored the <i>tetM</i> gene. These results indicate that <i>P. pentosaceus</i> SMFM2016-WK1 might control diarrheal pathogens isolated from pigs. However, a further study is necessary because the results were obtained only from <i>in vitro</i> experiment.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"67 4","pages":"868-891"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12380015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth performance of broiler chickens fed diets containing granulated L-methionine compared with DL-methionine.","authors":"June Hyeok Yoon, Su Hyun An, Yangsu Kim, Hyeon-Jin Kim, Changsu Kong","doi":"10.5187/jast.2024.e49","DOIUrl":"https://doi.org/10.5187/jast.2024.e49","url":null,"abstract":"<p><p>A novel granulated L-methionine (Met) has been developed using a simplified purification process, however its replacement with DL-Met has not yet been explored. The objective of the present study was to investigate the growth performance of broilers fed diets containing granulated L-Met (90% purity) compared to a diet containing DL-Met (99% purity). A total of 192 one-day-old broilers were allocated in four dietary treatments with six replicates (eight birds/cage) in a randomized complete block design based on body weight as the blocking factor. Twelve experimental diets were used, with four for each of the three growth stages: pre-starter (day 0 to 7), starter (day 7 to 21), and grower (day 21 to 28). The experimental diets consisted of: (1) a diet containing DL-Met at 100% of the digestible Met requirement, (2) a diet containing granulated L-Met at 85% of the digestible Met requirement, (3) a diet containing granulated L-Met at 90% of the digestible Met requirement, and (4) a diet containing granulated L-Met at the same inclusion rate (approximately 95% of the digestible Met requirement) as diet 1. The broilers were fed experimental diets during the pre-starter, starter, and grower stages, and growth performance was recorded by correcting mortality throughout the experiment period. Over the entire 28-day period, body weight gain and feed intake of broilers fed diets containing granulated L-Met increased linearly (<i>p</i> < 0.05) with an increase in dietary granulated L-Met supplementation. However, the growth performance of broilers fed diets containing granulated L-Met did not differ from those fed a diet containing DL-Met. The bioefficacy of L-Met relative to DL-Met for body weight gain and gain-to-feed ratio during the pre-starter stage was 116.9% and 104.0%, respectively. During the starter stage, the bioefficacy of L-Met relative to DL-Met was 127.5% and 111.0% for body weight gain and gain-to-feed ratio, respectively. Results of the present study reveal that the growth performance of broilers fed diets containing granulated L-Met was comparable to those fed a diet containing DL-Met, despite the lower dietary Met intake than digestible Met requirement. This suggests that L-Met might exhibit greater bioefficacy relative to DL-Met.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"67 4","pages":"817-825"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12380026/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alternative nutritional evaluation of black soldier fly with various substrates in cat diets and its effects on fecal microbiota.","authors":"Kyeongho Jeon, Jihwan Lee, Minho Song, Kwanho Park, Seyeon Chang, Dongcheol Song, Hyuck Kim, Jinmo Yang, Jinho Cho","doi":"10.5187/jast.2025.e58","DOIUrl":"https://doi.org/10.5187/jast.2025.e58","url":null,"abstract":"<p><p>This study aimed to examine the effects of proper substitution ratios and organic substrates of black soldier fly larvae (<i>Hermetia illucens</i> larvae; HIL) on cats. A total of 30 mixed-sex domestic cats (<i>Felis domestica</i>) with an initial body weight of 5.06 ± 0.89 kg were used in this experiment, which used an incomplete 5 × 5 replicated Latin square design. The experimental period was 10 d, with 7 d of diet adaptation followed by 3 d of dietary treatment. The dietary treatments were provided: 1) CON (basal diet), 2) HA3 (replacing 3% of the poultry meal (PM) in the basal diet with HIL fed on animal-based substrate), 3) HA6 (replacing 6% of the PM in the basal diet with HIL fed on animal-based substrate), 4) HP3 (replacing 3% of the PM in the basal diet with HIL fed on plant-based substrate) 5) HP6 (replacing 6% of the PM in the basal diet with HIL fed on plant-based substrate). The HA3 group showed significantly higher (<i>p</i> < 0.05) crude protein digestibility than the CON group. The groups replaced by HIL had significantly higher (<i>p</i> < 0.05) ether extract digestibility than the CON group. The leucine, tryptophan, serine, and aspartic acid digestibility were significantly increased (<i>p</i> < 0.05) in the groups replaced by HIL compared to the CON group. The HP6 group showed distinct (<i>p</i> < 0.05) unweighted UniFrac distances from all other groups and weighted UniFrac distances from the CON and HP3 groups. In addition, the CON group exhibited significant differences (<i>p</i> < 0.05) in weighted UniFrac distances compared to all treatment groups. At the genus level, the HP6 group showed increased (<i>p</i> < 0.05) abundance of Bacteroidota compared with the CON group, while the CON exhibited higher (<i>p</i> < 0.05) levels of Firmicutes than the HP6 group. In conclusion, substituting PM with HIL in cat diets improved nutrient digestibility and had no negative impact on the fecal microbiota. Therefore, it is considered safe and effective to substitute up to 6% of the protein source with HIL in cat diets.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"67 4","pages":"922-943"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12380016/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144954983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of polyphosphates with different chain lengths on digestive organ weight, carcass quality, and immune response, and intestinal microflora in broilers.","authors":"Yi-Qiang Chang, Seung-Gyu Moon, Yan-Qing Wang, Sang-Woo Jeon, Ah-Ran Lee, Soo-Hyun Kim, Won-Uk Hwang, Soo-Ki Kim","doi":"10.5187/jast.2024.e57","DOIUrl":"https://doi.org/10.5187/jast.2024.e57","url":null,"abstract":"<p><p>Physiological effects of polyphosphates with different chain lengths were unknown in poultry. The effect of 0.05% concentration of short chain polyphosphates (SCPP), medium chain polyphosphates (MCPP) and long chain polyphosphates (LCPP) was observed in broilers. MCPP and LCPP produced bacteriostatic properties against four pathogenic bacteria, <i>Shigella sonnei</i>, <i>Pseudomonas aeruginosa</i>, <i>Salmonella enterica</i> serovar. Pullorum, and <i>Escherichia coli</i> O157:H7. SCPP reduced the level of triglycerides in the blood. Intervention of MCPP and LCPP induced cecum IL-1β expression involved in the regulation of autoimmune inflammation. In terms of colony-forming units, SCPP increased the number of <i>Lactobacilli</i> while MCPP and LCPP significantly decreasing the number of <i>Shigella</i>, <i>Salmonella</i> and <i>Coliform</i> bacteria. SCPP, MCPP, and LCPP improved the intestinal microflora with abundance of beneficial bacteria such as <i>Faecalibacterium</i>, <i>Phocaeicola</i>, and <i>Barnesiella</i> but with reduced <i>Bacteroides</i>. In addition, SCPP, MCPP, and LCPP did not adversely affect the meat quality of broilers. The antimicrobial properties of SCPP, MCPP, and LCPP can help to improve the intestinal environment and enhance immune properties. Based on the comparison of different length polyphosphates in broiler chickens, it is suggested that MCPP is more effective compared to SCPP and LCPP as antimicrobial feed additives.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"67 4","pages":"853-867"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12380029/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geographical distribution and phenotypic characterization of Malin sheep in three selected states of Peninsular Malaysia.","authors":"Siti Nabilah Mahdzar, Mohd Aftar Abu Bakar, Nadiatur Akmar Zulkifli, Mahanem Mat Noor, Mohd Hafiz Abdul Rahman, Norfarhan Mohd-Assa'ad, Shairah Abdul Razak","doi":"10.5187/jast.2023.e80","DOIUrl":"10.5187/jast.2023.e80","url":null,"abstract":"<p><p>Conservation of indigenous breeds of ruminants is crucial for offering alternatives to commercial breeds. Moreover, it is part of long-term strategies in the agri-food sector to sustain supplies by ensuring genetic resource diversity to overcome climate change and the food crisis. Malin is the only native sheep breed in Malaysia. Due to traits such as heat tolerance and disease resistance, Malin sheep are considered an invaluable biological heritage. However, breeders and industrial producers are not interested in Malin because of their low commercial value and slow growth rate. Hence, this breed is neglected, its population is fragmented, and its numbers are dwindling, without data updates. Therefore, current information regarding Malin sheep is needed, including the latest geographical distribution and phenotypic characterization. First, we determined the population distribution using information from the State Department of Veterinary Services. Data were then collected from 15 studied locations in Pahang, Perak, and Kelantan via purposive sampling. Six qualitative traits and seven morphometric traits were recorded for 152 Malin sheep. These traits were quantitatively analyzed using multi-variate statistical tools to define the best measure to represent body conformation when comparing Malin sheep across studied locations. Findings showed that the Malin Ne population is very small. Morphologically, most Malin sheep exhibit light-brown wool with a course wool type; convex head shape, curved and horned in males but polled in females; and white hoof color. Imputation for missing body weight values in one population was successfully performed based on imputation regression modelling prior to downstream analyses. Kruskal-Wallis tests indicated that the median value of all morphometric traits except female body weight differed significantly between all studied locations. The highest correlation was observed between chest girth and body length in males (ρ=0.76) and chest girth and body weight (ρ=0.76) in females. Non-metric dimensional scaling showed that sheep maintained by smallholders in Pahang and Kelantan are similar phenotypically, but with smaller size compared with Perak. These findings suggest that phenotypic traits can help evaluate and compare sheep body conformation and thus provide an opportunity to distinguish and clarify a herd's position, thereby highlighting populations requiring management attention.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"10 1","pages":"737-758"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12380030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76797234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete genome sequence of <i>Priestia megaterium</i> S188, a hydrogen sulfide-degrading bacterium.","authors":"Sang Hoon Kim, Ji Hoon Song, Remilyn M Mendoza, Dae-Kyung Kang","doi":"10.5187/jast.2024.e81","DOIUrl":"10.5187/jast.2024.e81","url":null,"abstract":"<p><p><i>Priestia megaterium</i> (formerly <i>Bacillus megaterium</i>) is a gram-positive, aerobic, spore-forming bacterium found in a wide range of environmental niches. Here, we report the complete genome sequence of <i>P. megaterium</i> S188 isolated from soil, which can decrease hydrogen sulfide (H₂S) levels and help reduce malodor generation in livestock farms. Putative genes related to sulfide assimilation and conversion were found in the genome of <i>P. megaterium</i> S188; among these, one O-acetylhomoserine (O-AH) desulfhydrase, two cysteine synthases-primarily related to the biosynthesis of sulfur-containing amino acids, five rhodanese or sulfurtransferases, and one nitrogen reductase were identified. The genomic information on <i>P. megaterium</i> S188 provides insights into the possible biodegradation or conversion mechanisms of sulfur-containing substances that cause malodors, which can help reduce odor generation. Furthermore, identification of the key genes or molecules responsible for H₂S reduction would facilitate the optimization of the H₂S-degrading ability of S188.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"67 3","pages":"714-717"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12159701/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}