Journal of Animal Science and Technology最新文献

{"title":"Effects of illite or bentonite on cytotoxicity, antibacterial and adsorption capacity in porcine intestinal epithelial cells.","authors":"Seyeon Chang, Jihwan Lee, Seungwon Jung, Dongcheol Song, Sehyun Park, Kyeongho Jeon, Hyuck Kim, Jinho Cho","doi":"10.5187/jast.2024.e107","DOIUrl":"https://doi.org/10.5187/jast.2024.e107","url":null,"abstract":"<p><p>This study investigated the cytotoxicity of illite and bentonite using porcine intestinal epithelial cells (IPEC-J2) as a primary screening verification method for the effect of supplementing illite and bentonite. Also, it evaluated the antibacterial activity and adsorption capacity of illite and bentonite by infecting IPEC-J2 cells with pathogenic <i>Escherichia coli</i>. The addition levels of illite and bentonite in IPEC-J2 cells medium were set as follows; CON, 0%; I1, illite 1%, I1.5, illite 1.5%; I2, illite 2%; B1, bentonite 1%; B1.5, bentonite 1.5%; B2, bentonite 2%. The treatment time of illite and bentonite on IPEC-J2 cells was set to 2 hours, 4 hours, and 6 hours. Cytotoxicity and wound healing assays were analyzed without <i>E. coli</i> challenge. Cell viability and <i>E. coli</i> adhesion ability were assessed through illite or bentonite treatment with <i>E. coli</i> challenge. All experiments were performed with three independent replicates, with six technical replicates per experiment. The B1.5 and B2 treatment groups showed higher (TRT, <i>p</i> < 0.05) cytotoxicity than the CON and illite groups. There was no significant difference in cytotoxicity according to the illite and bentonite treatment time. For wound closure rate, when illite and bentonite were treated for 6 hours, a significantly higher (Time, <i>p</i> < 0.05) healing rate was shown than that of the 2 and 4-hour treatments. The B1 with <i>E. coli</i> challenge group showed a significantly higher (TRT, <i>p</i> < 0.05) live cell count than the non-supplementation with <i>E. coli</i> challenge and B2 with <i>E. coli</i> challenge groups. In conclusion, except for 1.5% and 2% addition levels of bentonite, the illite and bentonites did not induce cytotoxicity in IPEC-J2 cells regardless of the treatment time. They were thought to be effective in wound healing ability in IPEC-J2 cells.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 2","pages":"487-498"},"PeriodicalIF":3.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13071718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147690149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics integrated approach reveals host-microbiome interactions in the adaptive mechanisms of weaning piglets.","authors":"Ji-Yeong Lee, Chiwoong Lim, Young-Jun Seo, Hyunjin Kyoung, Sanghoon Lee, Younghoon Kim, Minhye Shin, Minho Song, YounChul Ryu, Jun-Mo Kim","doi":"10.5187/jast.2500436","DOIUrl":"https://doi.org/10.5187/jast.2500436","url":null,"abstract":"<p><p>The weaning transition is a critical phase in piglet development, marked by physiological challenges that influence growth and health. Therefore, this study aims to investigate host-microbiome interactions during the weaning transition using a multi-omics integrated approach. Fecal samples were collected from piglets on the weaning day (W0), 7 days post-weaning (W7), and 14 days post-weaning (W14). Ileal microbiota, microbial-derived metabolites, and tissue samples (ileum, thymus, and mesenteric lymph nodes) were collected at W0 and W14. Fecal microbiota analysis revealed a more stable community at W14 than at W7, with increased presence of fiber-degrading bacteria, including <i>Prevotella</i>, <i>Treponema</i>, <i>Muribaculaceae</i>, and <i>Lachnospiraceae</i>. The ileal microbiota exhibited an adaptive pattern with increases in <i>Lactobacillus</i>, <i>Clostridium_sensu stricto_1</i>, and <i>Enterobacteriaceae</i>, optimized for solid feed digestion and gut stabilization. Morphological analysis of the ileum showed changes in villus architecture between W0 and W14, including increased crypt depth and villus area and decreased villus width, while villus height and goblet cell counts were numerically higher at W14. Transcriptomic profiling revealed the ileum as the primary site of molecular adaptation, with 506 differentially expressed genes (DEGs) involved in immune response pathways, including viral protein interactions with cytokine and cytokine receptor pathways and T cell receptor signaling. The thymus (158 DEGs) and mesenteric lymph nodes (30 DEGs) exhibited modulation of structural pathways linked to systemic immune development, indicating tissue-specific molecular adaptation. Integrated analysis of the host transcriptome and microbial-derived metabolites revealed upregulated glycerophospholipid and glutathione metabolic pathways in piglets 14 days post-weaning, consistent with modulation of membrane structure, barrier function, and antioxidant defense during gut adaptation. Overall, the multi-omics findings provide a comprehensive description of molecular changes associated with weaning adaptation and identify candidate targets for piglet health management during the weaning transition.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 2","pages":"603-628"},"PeriodicalIF":3.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13071673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147690169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying the optimal ratios for replacing spray-dried plasma protein with hydrolyzed porcine intestinal protein in weaning pig.","authors":"Sehyun Park, Jihwan Lee, Seonwoong Kim, Hyuck Kim, Dongcheol Song, Seyeon Chang, Kyeongho Jeon, Jinmo Yang, Jihong Jung, Jinho Cho","doi":"10.5187/jast.2024.e120","DOIUrl":"https://doi.org/10.5187/jast.2024.e120","url":null,"abstract":"<p><p>This study aimed to evaluate the effects of replacing spray-dried plasma protein (SDPP) with hydrolyzed porcine intestinal protein (HP) in weaning pigs and determine the optimal replacement ratio. Ninety-six crossbred weaning pigs (initial body weight 7.35 ± 0.67 kg) were used for five weeks and assigned to four dietary treatments: HP0 (100% SDPP), HP25 (25% HP), HP50 (50% HP), and HP100 (100% HP), with 6 replicates of 4 pigs per treatment. The HP0 and HP25 diets significantly increased (<i>p</i> < 0.05) average daily gain (ADG) and feed efficiency (G:F) compared with HP100 at weeks 3 to 5 and over the entire study period. Increasing levels of HP replacement linearly decreased (<i>p</i> < 0.05) ADG and G:F. At week 3, HP0 and HP25 diets significantly increased crude protein (CP) digestibility compared to HP50 and HP100, with similar results observed at week 5. Additionally, HP0 and HP25 diets led to significantly lower (<i>p</i> < 0.05) total protein (TP) and blood urea nitrogen (BUN) levels than HP50 and HP100 at week 5. TP and BUN levels increased linearly as HP levels increased. The HP25 diet notably increased <i>Lactobacillus</i> counts in feces compared to HP50 and HP100 at week 5. Total weight gain was significantly higher (<i>p</i> < 0.05) in the HP0 and HP25 groups compared to HP100, and the HP25 diet significantly reduced feed cost per kg gain (FCG) compared with HP100. Moreover, during the study, the HP25 diet showed a trend (<i>p</i> = 0.087) towards lower FCG compared with HP0. In conclusion, replacing 25% of SDPP with HP provides optimal benefits in growth performance, nutrient digestibility, and feed cost efficiency without negative impacts on weaning pigs.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 2","pages":"516-531"},"PeriodicalIF":3.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13071670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147690218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of probiotics on growth performance, cytokine levels, and gut microbiome composition of broiler chickens for 7 and 35 days.","authors":"Jin Young Jeong, Junsik Kim, Minji Kim, Yoo-Bhin Kim, Cheolju Park, Jaekyeong Song, Dong Wook Kim, Minseok Kim, Nam-Geon Park","doi":"10.5187/jast.2025.e12","DOIUrl":"https://doi.org/10.5187/jast.2025.e12","url":null,"abstract":"<p><p>Through microbial fermentation, probiotics are essential for improving growth performance and gut health in broiler chickens. This study aimed to assess the effects of three additives on growth performance, cytokine levels, and cecal microbiota in broiler chickens. One-day-old Arbor Acres chicks (total 300) were randomized into four groups: (1) control: basal diet, (2) <i>Bacillus subtilis</i> (BS) + basal diet, (3) essential oil + basal diet, and (4) <i>Bacillus velezensis</i> + basal diet. All chickens were fed and watered ad libitum throughout the experiment. Feed intake and body weight were measured weekly. On days 7 and 35, cecal contents of one bird per replicate, based on average body weight, were collected and analyzed for microbiota using 16S rRNA gene amplicon sequencing. The BS group exhibited enhanced growth performance, including increased final body weight, average daily gain, and reduced feed conversion ratio compared to that of the other groups. On day 7, the BS group exhibited a higher abundance of <i>Eisenbergiella</i> (8.24%), and on day 35, there was an increased abundance of <i>Firmicutes</i> (99.63%) and <i>Lachnoclostridium</i> (1.4%). These results indicate that <i>B. subtilis</i> may be a promising probiotic for enhancing broiler health by modulating gut microbiota.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 2","pages":"586-602"},"PeriodicalIF":3.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13071723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147690144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hastening meat production: a review of growth promoters for traditional and cultured meat production.","authors":"Ermie Buen Mariano, Da-Young Lee, Ji Won Park, Seok Namkung, So Young Choi, Chae Hyeon Bok, Woojin Lee, Yewon Shin, Colin Venter, Sun Jin Hur","doi":"10.5187/jast.2500296","DOIUrl":"https://doi.org/10.5187/jast.2500296","url":null,"abstract":"<p><p>Time is an intangible yet important element in food production systems. It is often used as one of the bases for technology and production efficiency, with shorter production durations being favored. Meat is a staple source of protein for the human diet; therefore, efforts to improve and optimize production efficiency are always warranted. Growth promoters in traditional meat production are additives used to improve the performance and productivity of livestock animals while the enhancement of proliferation, differentiation, and maintenance capacity of cells are prioritized in cultured meat production. Although often put in opposition to each other, traditional and cultured meat share a common purpose of providing meat and its products for food security. This review provides insight into the previous and current growth promoters used in both meat production systems. Differences in traditional meat and cultured meat production, in terms of nutrient allocation and production period, were given emphasis. Potential effects of shortened meat production duration to the environment were also discussed. In both production systems, plant-derived growth promoters are commonly used to improve production efficiencies and food safety, particularly in terms of antimicrobial resistance, hormonal residues, and toxicity. Antioxidants play an important role in both meat production and myogenesis, supporting animal and cell growth. Shortened production duration due to growth promoters can generally lead to lower production costs, as well as result in the commercialization and price competitiveness of traditional and cultured meat products.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 2","pages":"399-419"},"PeriodicalIF":3.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13071691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147690201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole genome sequence analysis of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> KL101 and comparative genomics with BB12.","authors":"Kiyeop Kim, Junghee Lee, Seung-Ji Kang, Sejong Oh","doi":"10.5187/jast.2024.e76","DOIUrl":"https://doi.org/10.5187/jast.2024.e76","url":null,"abstract":"<p><p><i>Bifidobacterium</i> species is a prominent bacterium in the human gut, particularly in infants, where it plays an important role in maintaining gut health. The whole genome sequence of <i>B. animalis</i> subsp. <i>lactis</i> KL101 (KL101), isolated from infant feces, exhibits a compact structure with a genome size of approximately 1.92 Mbp comprising 1,555 coding sequences. Key chromosomal characteristics are genes encoding bile salt hydrolase and the thioredoxin system, which contribute to bile acid resistance and the oxidative stress response, respectively. Moreover, the genome has a significant number of genes that play a role in carbohydrate metabolism, supporting its probiotic functions. The comparative genomic analysis of the KL101 strain, in comparison to the well-known BB12 strain (<i>B. animalis</i> subsp. <i>lactis</i> BB12), reveals unique and similar characteristics. Although both strains have a similar guanine-cytosine (GC) content, KL101 exhibits unique genomic characteristics that may contribute to its specific adaptations in the infant gut. The results demonstrate that KL101 is highly adapted, with a genome specifically designed to efficiently process carbohydrates, withstand stress, and interact with its host. These findings enhance our understanding of KL101, supporting its potential applications in dietary supplements and health foods aimed at improving gut health.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 2","pages":"638-643"},"PeriodicalIF":3.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13071689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147690216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the origin, characters, and breeding system of Kebumen Ongole Grade cattle in Indonesia: a review.","authors":"Subiharta Subiharta, Pita Sudrajad, Yudi Adinata, Eni Siti Rohaeni, Rini Nur Hayati, Agung Prabowo, Salfina Nurdin Ahmad, Santiananda Arta Asmarasari, Rudi Prasetyo Hantoro","doi":"10.5187/jast.2024.e121","DOIUrl":"https://doi.org/10.5187/jast.2024.e121","url":null,"abstract":"<p><p>Kebumen Ongole Grade cattle represent a distinctive local breed of beef cattle in Indonesia known for their adaptability to tropical climates and low-input farming methods. These cattle, which are descended from Indian Ongole cattle, have a long history of development. While these cattle have many similarities to their ancestors, they have evolved unique features and performance traits, particularly in the Kebumen region. Despite their recognized value, the population has declined as a result of crossbreeding with other cattle breeds and uncontrolled mating. This has raised concerns about genetic erosion and the future viability of the breed. These cattle are also distinguished by their large body size and high birth weight, which are associated with excellent reproductive and production performance. Despite these advantages, better management and feeding systems are required to maintain and improve breed quality. These cattle also have a successful breeding program, with certified progeny distributed nationwide. The comprehensive documentation in this study is intended to serve as the foundation for future policy formulation and sustainable breeding strategies. This review suggests that Kebumen Ongole Grade cattle represent a successful community-based breeding effort with room for growth. This study emphasizes the importance of preserving the breed's genetic integrity while addressing breeding management issues and production system sustainability.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 1","pages":"1-17"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12923259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147271107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional characteristics of a pig 2D intestinal organoid model as an <i>in vitro</i> platform for nutritional studies.","authors":"Sang Seok Joo, Bon-Hee Gu, Eunbyeol Lee, Eunseon Oh, Minji Kim, Hyunjung Jung, Myunghoo Kim","doi":"10.5187/jast.2024.e116","DOIUrl":"10.5187/jast.2024.e116","url":null,"abstract":"<p><p>Intestinal epithelial cell lines have been widely used in the field of biomedical and livestock research, and recently, the use of organoid systems has been attempted. However, they have several limitations as an <i>in vitro</i> platform in particularly in nutrition-related studies. Thus, this study aimed to compare the existing <i>in vitro</i> platform (IPEC-J2 cell line) with a three-dimension (3D) organoid model, and to understand the nutritional phenomena occurring in the intestinal lumen through the establishment and characterization of a two-dimension (2D) organoid model. By comparing the IPEC-J2 cell line and 3D intestinal organoids, we found differences in intestinal epithelial cell types, including nutrient-related enteroendocrine cells and enterocytes. 3D organoids have most of gut epithelial cell types, but IPEC-J2 did not. We further established a 2D organoid model with an exposed apical membrane and compared it with a 3D organoid model. The established 2D organoids had higher expression of enteroendocrine cells and enterocyte marker genes, and most genes were related to nutritional properties (nutrient transporters, hormones, and digestive enzymes). Fatty acids, one of the nutrients, were added to the two organoid models for comparison. Fluorescence image analysis confirmed that more fatty acids were absorbed by 2D organoids. Treatment with a long-chain fatty acid mixture increased the expression of fatty acid receptor (<i>FFAR1</i> and <i>FFAR4</i>) and hormone (<i>GCG</i>, <i>CCK</i>, and <i>PYY</i>) genes in 2D organoids but not in 3D organoids, leading to the activation of metabolic responses. The more facilitated metabolic process was observed in 2D organoids by increased mitochondria activity and ATP production. Our findings emphasize that pig intestinal organoid systems, particularly 2D organoid model, is better <i>in vitro</i> platform, particularly in nutrition-related studies. Compared with other <i>in vitro</i> platforms, 2D organoids can be used for studying intestinal epithelial cell-nutrient interactions structurally and characteristically. Our study provides a basis for utilizing a pig 2D intestinal organoid model as a potentially advanced <i>in vitro</i> system for intestinal epithelial cell-based nutritional research in domestic animals.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 1","pages":"236-257"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12902515/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146201652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating RNA-Seq-based differential gene expression during hair follicle development in Angora goat skin.","authors":"Senem Esin Selçuk, Ozge Ozmen, Reyhan Çolak","doi":"10.5187/jast.2024.e104","DOIUrl":"10.5187/jast.2024.e104","url":null,"abstract":"<p><p>Mohair, an important source of fiber, is only obtained from Angora goats. The important characteristics that determine the economic value of mohair are fiber diameter and quantity. In countries where mohair is produced, efforts are made to improve these characteristics. It is stated that hair follicle characteristics and/or genetic regulation mechanisms that form animal fibers directly affect fiber production and quality. In this study, it was aimed to determine the genes affecting mohair development in two varieties of Angora goat and the molecular mechanisms affecting these genes. The biopsy samples were collected during three distinct phases of the hair growth cycle: anagen (active growth, September), catagen (transition, January), and telogen (resting, March). The specific timing and location of the biopsies suggest a potential influence of seasonality or a controlled experimental design. RNA was isolated from these biopsy samples, and differentially expressed genes (DEGs) and the pathways affected by these genes were evaluated using the RNA sequencing method. It has been observed that the expression of <i>KRTAP8-1</i>, <i>KRTAP16.4</i>, and <i>KRTAP21-1</i> genes was quite high in the group in which catagen and telogen phases were compared in Eskisehir variety females. Interestingly, the <i>KRTAP21-1</i> gene was found to be expressed in four different protein isoforms. Interestingly, the analysis revealed a cluster of keratin-associated protein genes (<i>KRT40</i>, <i>KRT72</i>, <i>KRTAP10</i>) solely differentially expressed (DE) in the male Eskisehir versus Lalahan comparison. This suggests potential sex-specific regulatory mechanisms involving keratinocyte differentiation during the catagen phase, which might be unique to the Eskisehir variety. In the Eskisehir variety, unlike the Lalahan variety, DEGs identified in the anagen-catagen comparison in males were significantly enriched in the Reactome mediated keratinization pathway, and these genes were down regulated. The results showed that <i>KRT</i> and <i>KRTAP</i> genes are highly functional and have different expression patterns between males and females, in addition to being different between the Eskisehir and Lalahan varieties. This study provides valuable insights into the genetic regulation of mohair development, potentially paving the way for targeted breeding strategies to improve mohair quality and production.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 1","pages":"72-95"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12902665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146201639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increasing arginine supplementation alleviated heat stress and citrulline can effectively substitute arginine in broilers.","authors":"Ji Hwan Lee, Dong Cheol Song, Seung Won Jeong, Se Yeon Chang, Young Gi Hong, Ji Soo Tak, Kyeong Ho Jeon, Hyuck Kim, Jin Ho Cho","doi":"10.5187/jast.2024.e94","DOIUrl":"10.5187/jast.2024.e94","url":null,"abstract":"<p><p>This study was conducted to determine the optimal standard ileal digestible (SID) arginine (Arg) to SID lysine (Lys) ratio in broilers under cyclic heat stress. Additionally this study tested whether citrulline (Cit) can replace Arg under cyclic heat stress, based on the report that a large amount of Arg is metabolized in the liver while Cit can by-pass metabolism in the liver. A total of 360, one-day-old Arbor Acres broiler chickens with initial body weight of 34.50 ± 0.87 g were placed in 24 pens. The 24 pens were randomly assigned to four dietary treatments with six replicates of fifteen broiler chickens. Treatments were as follows: 1) NC (SID Arg : Lys =0.95), 2) PC (SID Arg : Lys=1.05), 3) Arg1.15 (SID Arg : Lys =1.15), 4) Arg1.25 (SID Arg : Lys =1.25), 5) Cit33 (supplementation of Cit at 33% of Arg supplementation in Arg1.15, 6) Cit50 (supplementation of Cit at 50% of Arg in Arg1.15). The Arg1.25 group had the highest BW on 32 days and BWG during the overall period (<i>p</i> < 0.05) than the NC groups. However, there was no significant difference (<i>p</i> > 0.05) on day 32 BW and BWG during the overall period in Arg supplemented groups (Arg1.15 and Arg1.25) and Arg replacement with Cit groups (Cit33 and Cit50). Arg1.25 and Cit33 groups had higher villus height (VH) in the duodenum, jejunum and ileum than the NC groups. Moreover, the Arg1.25 group had the lowest crypt depth (CD) in the jejunum and ileum than the NC group, while there was no significant difference (<i>p</i> > 0.05) between Arg supplementation and Arg replacement with Cit groups. Arg1.25 group had the highest arginase activity in the liver and total nitric oxide synthase (NOS) and arginase activity in the kidney than other treatments, but no statistical difference was observed (<i>p</i> > 0.05) in arginase in the liver among treatments. Collectively the results ascertain that Cit can effectively replace a certain part of dietary arginine in broiler diets.</p>","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"68 1","pages":"151-167"},"PeriodicalIF":3.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12902513/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146201644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}