Journal of Advanced Veterinary and Animal Research最新文献

{"title":"The frozen-thawed sperm protein of Indonesian Toraya buffaloes is significantly associated with sperm kinematics, acrosome integrity, and mitochondrial membrane potential.","authors":"Tulus Maulana, Syahruddin Said, Raden Iis Arifiantini, Jakaria Jakaria, Asep Gunawan","doi":"10.5455/javar.2024.k838","DOIUrl":"10.5455/javar.2024.k838","url":null,"abstract":"<p><strong>Objective: </strong>The study aimed to characterize frozen-thawed sperm proteins and their correlation with sperm kinematics, acrosome integrity, and mitochondrial membrane potential (MMP) in Indonesian Toraya buffalo bulls.</p><p><strong>Materials and methods: </strong>Semen was obtained from six Toraya buffaloes classified as Saleko and Bonga 4-10 years old. The frozen semen was analyzed for sperm motility, sperm kinematics, viability, abnormalities, membrane integrity, intact acrosome, MMP, DNA integrity, and sperm protamine (PRM) deficiency. Sperm protein concentration (Prot. Con) was determined by the bicinchoninic acid, and protein molecular weight (MW) was determined using 1D sodium dodecyl sulfate-polyacrylamide gel electrophoresis with 4%-20% gradient gel and 6.5-240 kDa protein marker.</p><p><strong>Results: </strong>The results of this study showed that the quality of frozen semen from Toraya buffalo bulls is in a good category and suitable for use in artificial insemination programs. The sperm quality differed significantly (<i>p <</i> 0.05) between individuals, immunofluorescence examination of intact acrosome, PRM deficiency, intact MMP, and intact DNA showed no significant difference (<i>p <</i> 0.05)<i>.</i> Pearson correlations in this study showed that sperm Prot. Con has a significant correlation (<i>p <</i> 0.05) with acrosome integrity. The sperm protein band (Prot. Band) correlated significantly (<i>p <</i> 0.05) with sperm kinematic parameters and intact MMP. The average sperm Prot. Con of Toraya buffalo was 77.29 <i>±</i> 39.26 µg/ml and 4-13 Prot. Bands with 6-240 kDa of MW were detected, with Prot. Bands of 16, 50, 70, and 115 kDa having higher intensity.</p><p><strong>Conclusions: </strong>Frozen-thawed sperm protein is correlated with sperm kinematics, acrosome integrity, and MMP. The proteins were found to correlate with sperm quality and fertility in Toraya buffalo bulls.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 4","pages":"869-879"},"PeriodicalIF":1.5,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aflatoxin B1, ochratoxin A, and fumonisin B1 detoxification from poultry feeds by corona discharge application.","authors":"Hiba S Alnaemi, Tamara N Dawood, Qais Th Algwari","doi":"10.5455/javar.2024.k834","DOIUrl":"10.5455/javar.2024.k834","url":null,"abstract":"<p><strong>Objective: </strong>The efficiency of corona discharge (CD) for detoxification of aflatoxin B1 (AB1), ochratoxin A (OA), and fumonisin B1 (FMB1) from poultry feeds with its influences on feed components was investigated.</p><p><strong>Materials and methods: </strong>Feed samples were exposed to CD for six durations (10, 20, 30, 40, 50, and 60 min) at three distances (1.5, 2.5, and 3.5 cm). Mycotoxin levels were estimated by competitive enzyme-linked immunosorbent assay, and findings were substantiated by high-performance liquid chromatography.</p><p><strong>Results: </strong>AB1, OA, and FMB1 degradation percentages increased significantly (<i>p</i> < 0.05) with processing times increment and distances reduction to reach values of 83.22%, 84.21%, and 84.76% at the first distance; 80.28%, 84.00%, and 84.12% at the second distance; and 68.30%, 71.74%, and 76.18% at the third distance, respectively, after 60 min of treatment. FMB1 reported the highest degradation level. Concerning CD impacts on feed composition, protein, fat, and moisture contents decreased significantly (<i>p</i> < 0.05). Carbohydrates and ash were not affected adversely. Depending on peroxide values estimation, fats were of good quality.</p><p><strong>Conclusion: </strong>The CD effectiveness for AB1, OA, and FMB1 detox from poultry feeds with moderate impact on the quality of feed.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 4","pages":"819-834"},"PeriodicalIF":1.5,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of sperm motility, acrosome integrity, protamine deficiency, and DNA fragmentation in proven and unproven Friesian Holstein bulls.","authors":"Ristika Handarini, Abdullah Baharun, Annisa Rahmi, Deden Sudrajat, Anggraeni Anggraeni, Nurcholis Nurcholis, Hikmayani Iskandar, Tulus Maulana, Ekayanti Mulyawati Kaiin, Saiful Anwar, Syahruddin Said","doi":"10.5455/javar.2024.k831","DOIUrl":"10.5455/javar.2024.k831","url":null,"abstract":"<p><strong>Objective: </strong>The evaluation of frozen semen quality is an essential aspect in determining male fertility for artificial insemination programs. This study aims to evaluate the characteristics of Friesian Holstein (FH) bull-frozen semen in different classes (proven and unproven) based on protein profiling and molecular evaluation.</p><p><strong>Materials and methods: </strong>This study used frozen semen straws from FH bulls selected according to criteria for proven (6 individuals) and unproven (6 individuals) bulls produced by the Singosari AI Center (AIC). Sperm motility parameters were assessed using Computer Assisted Semen Analysis (CASA Supervision^®, Germany), while sperm viability and abnormality were evaluated through eosin-nigrosin staining under a microscope at 400´ magnifications. The integrity of the sperm plasma membrane was determined using the hypoosmotic swelling test, and acrosome integrity was analyzed using the fluorescein isothiocyanate PNA-propidium iodide staining method. Protamine deficiency was quantified using Chromomycin A3 fluorescence staining, while DNA fragmentation was assessed using the acridine orange technique.</p><p><strong>Results: </strong>The findings demonstrated that there were no statistically significant differences (<i>p</i> > 0.05) in the assessed parameters of frozen semen quality between FH-proven and unproven bulls. Furthermore, in FH-proven bulls, a negative correlation was observed between protamine deficiency and acrosome integrity (<i>r</i> = -0.528) and between protamine deficiency and sperm DNA fragmentation (<i>r</i> = -0.467). The parameters of protamine deficiency in unproven bulls exhibited a positive correlation with sperm progressive motility.</p><p><strong>Conclusion: </strong>The frozen semen quality of FH bulls in different classes (proven and unproven) was found to be equally good. Molecular-based analysis allows for a more accurate determination of semen quality. These findings are significant for bull breeding stations when comprehensively evaluating semen quality.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 3","pages":"796-802"},"PeriodicalIF":1.5,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal histological changes and aquaporin 5 immunoreactivity in the ductus deferens and prostate gland of Saudi Arabian dromedary camel.","authors":"Marwa Babiker","doi":"10.5455/javar.2024.k820","DOIUrl":"10.5455/javar.2024.k820","url":null,"abstract":"<p><strong>Objectives: </strong>The present study aims to investigate the seasonal histological structure changes and immunoreactivity of Aquaporin 5 (AQP-5) in the ductus deferens and prostate under the light microscope.</p><p><strong>Materials and methods: </strong>For the present study, twelve mature male camels were employed. Following the animals breeding and non-breeding seasons of slaughter under official licensing and veterinary supervision, samples were obtained from various regions of the ductus deferens and prostate gland and processed using general histology and immunohistochemical methods.</p><p><strong>Results: </strong>The secretory end pieces of the vas deferens ampulla and prostate gland of the Saudi Arabian dromedary camel were well developed during reproductive and non-reproductive seasons. The spermatozoa were noticed in the lumen of the ductus deferens during the reproductive and non-reproductive seasons. The response of AQP-5 in the ductus deferens was particularly significant throughout the not-rutting season. Moreover, during the non-breeding season, the prostate gland showed a strong to moderate response. In the breeding season, the ampulla of the ductus deferens showed an insignificant reaction. Additionally, the body and disseminated portion of the prostate gland showed a mild to moderate response.</p><p><strong>Conclusion: </strong>The results suggested that Saudi Arabian dromedary camels might produce sperm during rutting and non-rutting seasons.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 3","pages":"703-710"},"PeriodicalIF":1.5,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590585/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic status, reproductive, and productive performances of transition dairy cows as affected by dietary rumen-protected choline supplementation.","authors":"Ratchataporn Lunsin, Damrongchai Sokantat, Taina Silvestre, Helio Rezende Lima Neto, Thong Jin Koh, Fei Sun, Chittraporn Yeanpet, Ruangyote Pilajun","doi":"10.5455/javar.2024.k827","DOIUrl":"10.5455/javar.2024.k827","url":null,"abstract":"<p><p>Research articles about the effects of rumen-protected choline (RPC) supplementation on metabolic response, and reproductive and productive performances in transitional dairy cows were reviewed and presented. Analysis was conducted on 32 research papers that were published. The papers examined treatments without RPC supplementation and RPC supplementation levels varying from 10 to 100 gm/day. The feeding duration of RPC started from 40 to 140 days prepartum and continued until 20 to 140 days postpartum in multiparous dairy cows. Studies indicated that adding herbal choline (Cho) to the diet of transition dairy cows resulted in increased milk production and improved milk quality, leading to enhanced energy balance and reduced oxidative stress. The concentration and yield of IgG in colostrum provide passive immunity to dairy newborns and can be enhanced by dietary Cho supplementation. The inconsistent effects of RPC supplementation on reproduction may be due to several factors such as heat stress, genetics, and management. RPC supplementation improved the transition dairy cows' milk yield and quality, but dosage response was not observed as in the prior publication. Remarkably, the length of RPC supplementation had a positive correlation with an increase in milk yield. Based on this review, 45-50 gm/day dietary RPC supplementation between 3 weeks pre-calving to 8 weeks post-calving is suggested to increase at least 10% milk yield in dairy cows.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 3","pages":"754-761"},"PeriodicalIF":1.5,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590597/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Willingness to pay for certification and labeling of chicken meat in the Mymensingh District of Bangladesh.","authors":"Mahbuba Akther Mishu, Sourav Mohan Saha, Md Masudul Haque Prodhan, Md Mostafijur Rahman, Md Akhtaruzzaman Khan","doi":"10.5455/javar.2024.k829","DOIUrl":"10.5455/javar.2024.k829","url":null,"abstract":"<p><strong>Objective: </strong>This study explores consumers' willingness to pay (WTP) for labeled and certified chicken meat in Mymensingh district, examining the key socioeconomic and demographic factors that shape these preferences.</p><p><strong>Materials and method: </strong>Data were gathered through structured interviews with 300 participants from diverse urban and rural demographics, encompassing various occupational groups. The analysis employed logistic regression models to examine the determinants of WTP for labeling and certification, with a focus on variables such as income, education, health perception, environmental awareness, dependency ratio, and market distance.</p><p><strong>Results: </strong>The study found that a significant proportion of consumers preferred labeled (64%) and certified (71%) broiler meat. Strong links between higher income and education levels and increased WTP suggest that wealthier and more literate consumers are more likely to invest in food safety measures. Positive health and environmental perceptions also played a role, with more conscious consumers willing to pay a premium for labeled and certified products. On the other hand, a higher dependency ratio and greater market distance were associated with lower WTP, underscoring the impact of socioeconomic constraints and accessibility issues on consumer choices.</p><p><strong>Conclusion: </strong>Introducing labeling and certification systems could strengthen consumer trust and enhance public health, offering substantial benefits to both the poultry industry and the broader economy in Bangladesh.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 3","pages":"772-781"},"PeriodicalIF":1.5,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590609/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The protective effect of <i>Curcuma longa</i> on male infertility induced by thioacetamide.","authors":"Fatima S Alaryani, Fatima A Jaber, Boudor S Almutiri, Suzan B Abdu, Arif Mohammed, Asmaa H Al-Robiee","doi":"10.5455/javar.2024.k828","DOIUrl":"10.5455/javar.2024.k828","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the impact of thioacetamide (TAA) on the structure and function of the testes and assess the therapeutic effects of <i>Curcuma longa</i> (Cl) against TAA-induced toxicity in rats.</p><p><strong>Materials and methods: </strong>Thirty-two male albino rats weighing 180-200 gm and aged 11-12 weeks were randomly separated into four groups. The control group was given normal saline, the Cl group was orally administered Cl (500 mg/kg/day), the TAA group received intraperitoneal TAA (200 mg/kg body weight, three times/week), and the Cl with TAA group received Cl orally two hours before TAA administration. After 8 weeks, all rats were anesthetized, and body and testis weights were recorded. Morphological and histological assessments as well as biochemical analyses were conducted.</p><p><strong>Results: </strong>The study revealed a significant decrease in both body and testis weights in the TAA group, accompanied by a substantial increase in luteinizing hormone (LH), follicle-stimulating hormone (FSH), and malondialdehyde (MDA) levels. Testosterone (T) and glutathione (GSH) were significantly decreased in the TAA-treated group compared to the control. Conversely, the Cl-treated group exhibited a substantial decrease in LH, FSH, and MDA levels while showing a significant increase in T and GSH.</p><p><strong>Conclusion: </strong>Cl has been found to have a potential therapeutic role in mitigating TAA-induced testicular damage by acting as an antioxidant. This is supported by a significant decrease in oxidative stress markers and supporting hormonal levels. Further research is needed to understand the underlying mechanisms and explore the clinical applicability of Cl in preventing and treating testicular toxicity.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 3","pages":"762-771"},"PeriodicalIF":1.5,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of meleanocortin 4 receptor gene polymorphism (MC4R:C.92C>T) with feline diabetes.","authors":"Kristina Morkūnienė, Justina Dambrauskaitė, Renata Bižienė, Ramutė Mišeikienė, Nijolė Pečiulaitienė, Laimutis Kučinskas, Ugnė Dudonytė","doi":"10.5455/javar.2024.k825","DOIUrl":"10.5455/javar.2024.k825","url":null,"abstract":"<p><strong>Objective: </strong><i>Diabetes mellitus</i> (DM) is a prevalent endocrine condition found in cats. Recent research has identified a connection between a higher risk of diabetes in cats and genetic factors. This genetic susceptibility to type 2 diabetes is associated with genes that control insulin secretion and function, as well as those that impact factors like obesity. The purpose of the study was to determine the prevalence of the melanocortin 4 receptor (MC4R) gene missense mutation among cats and to investigate the relationship between body condition score (BCS) and the occurrence of diabetes in felines.</p><p><strong>Materials and methods: </strong>Genotype analysis was done for 45 samples. The research results were obtained with the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The 1,200-bp PCR product was cut with <i>BstOI</i> restriction enzyme.</p><p><strong>Results: </strong>Upon analyzing the data, it was found that 37.8% (<i>n</i> = 17) of the subjects carried the <i>MC4R</i> gene mutation. Across the entire population of cats studied, the C allele associated with the mutation was most prevalent in the group of cats with DM (frequency of 0.3) and least common among the clinically healthy cats (frequency of 0.2).</p><p><strong>Conclusion: </strong>After analysis of the study results, a statistically significant difference was observed between cats with DM and clinically healthy cats in the comparison of their BCS (<i>p</i> < 0.05). The association of the <i>MC4R</i> gene polymorphism with overweight and the development of diabetes has been identified.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 3","pages":"738-745"},"PeriodicalIF":1.5,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential ectoparasiticide for dog and cat fleas, a combination of <i>Ficus minahassae</i> extract and latex from <i>Carica papaya</i> L.","authors":"Dina Victoria Rombot, Yermia Semuel Mokosuli","doi":"10.5455/javar.2024.k833","DOIUrl":"10.5455/javar.2024.k833","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to analyze the compound content of the combined extracts of Langusei fruit (<i>Ficus minahassae</i> L.) and papaya latex (<i>Carica papaya</i> L.) and perform bioassays of the combination of extracts on fleas of the genus <i>Ctenocephalides</i>.</p><p><strong>Materials and methods: </strong>Liquid chromatography-tandem mass spectrometry (LC-MS/MS) determined the chemical composition of the combined extract. The toxicity of the extract combination was evaluated <i>in vivo</i> on cat flea and dog flea imago separately. The combination of phytochemical screening of Langusei extract and papaya latex contained all the main phytochemical compounds.</p><p><strong>Results: </strong>The LCMS/MS analysis results showed that the combination of Langusei extract and papaya latex contained six compounds. Four compounds were identified: 3-butenyl glucosinolate, erythromycin, aluminum palmitate, and henpentakontilbenzene. Four compounds indicate a new compound. The toxicity of the combined extract was highest for both <i>Ctenocephalides felis</i> and <i>Ctenocephalides canis</i> in the P4 treatment (10%), with an average mortality of 100%, the same as the control mortality of the synthetic insecticide deltamethrin. The best LD₅₀ for <i>C. felis</i> was in the F1 formula (4.003 mg/l), while in <i>C. canis</i> it was shown in the F3 (3.733 mg/l).</p><p><strong>Conclusion: </strong>Based on the results obtained, the combination of Langusei fruit extract and papaya latex can be developed as an ectoparasiticide for fleas of the genus <i>Ctenocephalides</i>.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 3","pages":"810-818"},"PeriodicalIF":1.5,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590603/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Welfare assessment of broiler chickens at live bird market of Chattogram in Bangladesh.","authors":"Md Ridoan Pasha, Mohammad Belayet Hossain, Amir Hossan Shaikat, Minhazur Rahman, Mohammad Rashedul Alam","doi":"10.5455/javar.2024.k832","DOIUrl":"10.5455/javar.2024.k832","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to assess the welfare conditions of broiler chickens in the live bird markets (LBMs) in Bangladesh.</p><p><strong>Materials and methods: </strong>A total of fifty broiler outlets were studied in 10 LBMs of Chattogram, Bangladesh. A total of 10 chickens were observed to check the welfare issues during slaughter from each outlet (<i>N</i> = 500). The data were collected using a structured questionnaire method through interviews of the vendors and observation of the lairage and slaughter practice.</p><p><strong>Results: </strong>The study revealed that the stocking density was significantly higher in cage-type lairage than in floor-type (<i>p</i> < 0.05). The feeding and drinking areas for the chickens were significantly but negatively correlated to the stocking density. The duration between unloading of broiler chickens at LBMs and feeding or drinking could exceed 5 hours in 22% of outlets. The mortality was significantly higher in the bigger outlets than the smaller outlets (<i>p</i> < 0.05). During pre-slaughter handling, the one-wing grasping method was practiced more in the bigger outlets (<i>p</i> < 0.05) whereas the feet grasping method was used more in the smaller outlets (<i>p</i> < 0.05). Moreover, the knives used to slaughter the chickens were not sharpened daily in 76% of outlets.</p><p><strong>Conclusion: </strong>This study indicated that the broiler chickens in the LBMs of Chattogram had to face many stress episodes at different stages at their penultimate moments-from lairage to slaughter-which led to poor welfare conditions and exacerbated the suffering of chickens.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 3","pages":"803-809"},"PeriodicalIF":1.5,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}