Journal of Advanced Veterinary and Animal Research最新文献

{"title":"Effects of waste-based concentrates from broiler farm on physico-chemical qualities and blood profile of landrace pigs.","authors":"Tirta Ariana, Djoko Kisworo, Bulkaini Bulkaini, Sumerta Miwada, Dewi Ayu Warmadewi, Rahayu Tanama Putri","doi":"10.5455/javar.2023.j712","DOIUrl":"10.5455/javar.2023.j712","url":null,"abstract":"<p><strong>Objective: </strong>The study aimed to determine the effect of giving concentrate protein from closed-house (CP-CH) broiler farm waste in rations on the meat quality (physical-chemical) and blood profile of landrace crossbreed pigs.</p><p><strong>Materials and methods: </strong>The study used a completely randomized design (CRD) with 3 treatments and 4 replications with 12 finisher-phase landrace pigs with an average body weight of 63.42 ± 2.39 kg. The treatments were: A (control): use of 0% CP-CH + 24% CP-152 concentrate; B: 12% CP-CH + 12% CP-152 concentrate; and C: 24% CP-CH + 0% CP-152 concentrate. The research parameters were meat quality (physical-chemical meat) and blood lipid profile [total cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and triglycerides (TGs)].</p><p><strong>Results: </strong>The results showed that treatment group B obtained physical meat quality (pH, drip loss, cooking loss, and water holding capacity) and meat chemical quality (moisture, protein, fat, and ash content) that were almost the same as the control (<i>p ></i> 0.05). By administering 24% CP-CH + 0% CP-152 (C) concentrate, there was a significant decrease in the physical and chemical quality of crossbreed landrace pigs (<i>p</i> < 0.05). Administration of CP-CH at 12%-24% did not affect total cholesterol, HDL, LDL, or blood TGs in landrace-crossbreed pigs.</p><p><strong>Conclusion: </strong>Giving 12% CP-CH (B) obtained meat quality (physical and chemical) almost the same as the control group. Treatment group C significantly reduced the physical and chemical quality of the meat. Administration of CP-CH at 12%-24% did not affect the blood profile of landrace crosses.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10868680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139899910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial resistance patterns of <i>Staphylococcus aureus</i> isolated from apparently healthy pet cats of Bangladesh.","authors":"Shanta Das, Ajran Kabir, Chandra Shaker Chouhan, Md Ahosanul Haque Shahid, Tasmia Habib, Md Alamgir Kobir, Md Zawad Hossain, Marzia Rahman, K H M Nazmul Hussain Nazir","doi":"10.5455/javar.2023.j708","DOIUrl":"10.5455/javar.2023.j708","url":null,"abstract":"<p><strong>Objective: </strong>This study sought to determine the occurrence, molecular identification, antimicrobial-resistant trends, and gene distribution of <i>Staphylococcus aureus</i> in pet cats and their owners' hand swabs.</p><p><strong>Materials and methods: </strong>From different places and clinics in Mymensingh and Dhaka, 168 pet cat samples and 42 hand swab samples from cat owners were obtained. The organisms were scrutinized by assessing the outcomes using conventional and molecular techniques. The disc diffusion technique was applied to find the resistance pattern against 12 antibiotics, and genes were discovered by targeting specific genes using PCR.</p><p><strong>Results: </strong>The occurrence of pathogenic <i>S. aureus</i> in pet cats was 7.74%, while it was 9.50% in pet owners' hand swabs, and 25.0% of the pet owner's hand swabs contained these genes. <i>Staphylococcus aureus</i> was utterly resistant to amoxicillin, ampicillin, cefixime, erythromycin, and imipenem in both pet cat and hand swabs of pet owner samples. All <i>S. aureus</i> isolates had a multidrug-resistant phenotype, and 1 from pet cats (O19) and 1 from pet owner hand swabs (H9) were resistant to all 12 antibiotics in the 7 antimicrobial classes. Several antibiotic-resistance genes were detected by PCR.</p><p><strong>Conclusion: </strong>The study confirmed multidrug-resistant pathogenic <i>S. aureus</i> in pet cats and their owners in Bangladesh, indicating a major health risk to both people and cats. Thus, a holistic and integrated one-health approach between veterinary and medical specialists is needed to mitigate the global distribution of these zoonotic antibiotic-resistant <i>S. aureus</i> strains.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134648960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of particle size of zeolite clay included in high-energy diets for feedlot lambs: Growth performance, dietary energy, carcass traits, and visceral mass.","authors":"Beatriz I Castro-Pérez, Jonathan Rodríguez-Vázquez, Alfredo Estrada-Angulo, Francisco G Ríos-Rincón, Jesús D Urías-Estrada, Elizama Ponce-Barraza, Alberto Barreras, Alejandro Plascencia","doi":"10.5455/javar.2023.j703","DOIUrl":"https://doi.org/10.5455/javar.2023.j703","url":null,"abstract":"<p><strong>Objective: </strong>Several reports on the effects of zeolite (ZEO) inclusion in diets for feedlot lamb diets have indicated improvements in dietary energy efficiency and decreases in internal fat. Inclusion levels and the type of zeolite used have been the main focus of those reports. However, the possible effect of the zeolite particle size on the growth performance and carcass characteristics has not yet been investigated.</p><p><strong>Material and methods: </strong>Forty-eight male intact Pelibuey × Katahdin lambs were fed for 67 days with a high-energy diet supplemented with ZEO as follows: 1) basal diet 0% ZEO (control); 2) basal diet 3% ZEO particle size 250 µM (sieve 60); 3) basal diet 3% ZEO particle size 149 µM (sieve 100); and 4) basal diet 3% ZEO particle size 74 µM (sieve 200). ZEO replaced corn grain and soybean meal in equal parts. At the end of feeding, lambs were slaughtered, and variables such as carcass characteristics and visceral mass were evaluated.</p><p><strong>Results: </strong>Particle size did not affect growth performance, carcass characteristics, or visceral mass. The inclusion of ZEO reduced 3.4% of dietary net energy in diets but did not affect dry matter intake or average daily gain, thus improving (<i>p</i> ≤ 0.05) dietary energy efficiency by 2.9%. Except for an 18.9% reduction (<i>p</i> = 0.02) in visceral mass fat, ZEO did not affect other carcass characteristics or visceral organ mass.</p><p><strong>Conclusion: </strong>The particle size studied in the present experiment has not affected any of the variables of growth performance or carcass characteristics. ZEO inclusion could be a strategy to reduce the visceral fat in finishing lambs.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636074/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134649020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and identification of caprine pasteurellosis in pneumonic goats in Bangladesh.","authors":"Md Habibur Rahman, Sonia Akther, Md Shahin Alam, Md Zakir Hassan, Md Samun Sarker, Md Zulfekar Ali, Md Giasuddin, Sadek Ahmed","doi":"10.5455/javar.2023.j707","DOIUrl":"10.5455/javar.2023.j707","url":null,"abstract":"<p><strong>Objective: </strong>This research aimed to assess the prevalence of caprine pasteurellosis, isolate and identify pasteurellosis (<i>Mannheimia haemolytica</i> and <i>Pasteurella multocida</i>) in pneumonic goats, and discover the main bacterial cause of pneumonia.</p><p><strong>Materials and methods: </strong>One hundred and five samples (94 nasal swabs and 11 lung tissues) from goats suspected of having pneumonia were taken and transferred aseptically to the laboratory. Following the processing of the collected samples, <i>Pasteurella</i> spp. was isolated with the aid of plate culture methods. Biochemical characteristics were used to identify all bacterial isolates, which were then verified by polymerase chain reaction (PCR). Antimicrobial susceptibility testing was also carried out to evaluate the sensitivity profiles of various antibiotics. The <i>Pasteurella haemolytica</i> serotype-specific antigen (PHSSA) gene was used to identify isolates of <i>M. haemolytica</i>, and the KMT1 gene was used to identify isolates of <i>P. multocida</i>.</p><p><strong>Results: </strong>From the 105 clinically suspicious samples, 51 (48.57%) were identified to be <i>Pasteurella spp.</i> through bacteriological testing and also by PCR targeting the <i>16S rRNA</i> gene. Of these, 47.87% (45/94) were nasal swabs, and 54.55% (6/11) were lung tissues. Among confirmed samples, 70.59% (36/51) were identified as <i>M. haemolytica,</i> and 29.41% (15/51) were identified as <i>P. multocida</i>. Resistance to tetracycline, streptomycin, oxytetracycline, gentamicin, and ceftriaxone was found in 50%-83% of the isolates. In addition, PCR identified the PHSSA and KMT1 genes from isolates of <i>P. multocida</i> and <i>M. haemolytica</i>, respectively.</p><p><strong>Conclusion: </strong>The present study revealed that <i>M. haemolytica</i> and <i>P. multocida</i> primarily caused pasteurellosis in pneumonic goats in Bangladesh. However, when treating these animals, the proper choice of antimicrobials should be made to control this disease.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134648969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of canine oral tumors: Insights into prevalence, types, and lesion distribution.","authors":"Chakkarin Satthathum, Supreeya Srisampane, Pollawat Jariyarangsrirattana, Pitak Anusorn, Panpicha Sattasathuchana, Naris Thengchaisri","doi":"10.5455/javar.2023.j709","DOIUrl":"https://doi.org/10.5455/javar.2023.j709","url":null,"abstract":"<p><strong>Objective: </strong>The escalating prevalence of canine oral tumors has emerged as a considerable health concern. This study examined the prevalence, types, and distributions of lesions linked to canine oral tumors.</p><p><strong>Material and methods: </strong>The medical records of 526 dogs diagnosed with oral tumors were analyzed to determine the prevalence, types, and distributions. Tumor stages were classified into four categories using the tumor node metastasis system.</p><p><strong>Results: </strong>Among the 526 dogs, there were 118 cases of benign tumors and 408 cases of malignant tumors. Acanthomatous ameloblastoma was the most common benign tumor (43.22%), while melanoma was the most common malignant tumor (51.23%). The gingiva was the most common site for both benign and malignant lesions, accounting for 89.83% and 63.73% of cases, respectively. Melanoma, squamous cell carcinoma, and fibrosarcoma were primarily located in the gingiva, whereas osteosarcoma was commonly found in the mandible. Most tumors were classified as stage III (ranging from 46.84% to 74.58%). Of the reported cases, 56.08% were males and 43.92% were females, and the most common breed was mixed at 30.41%, followed by Poodle at 14.25% and Shih Tzu at 11.40%. Moreover, patients with malignant oral tumors (11.6 ± 3.1 years) were significantly older than those with benign tumors (8.9 ± 3.4 years, <i>p</i> < 0.0001).</p><p><strong>Conclusion: </strong>Gingiva was the primary site for oral tumors, and mainly classified as stage III. These findings emphasize the increasing occurrence of oral tumors in senior and geriatric dogs and provide insights into the prevalent types and distribution.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636079/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134648963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete genome sequence analysis of the multidrug resistant <i>Aeromonas veronii</i> isolated for the first time from stinging catfish (Shing fish) in Bangladesh.","authors":"Mohummad Muklesur Rahman, Mohammad Sadekuzzaman, Md Asikur Rahman, Mahbubul Pratik Siddique, Mohammad Asir Uddin, Md Enamul Haque, Md Golam Azam Chowdhury, A K M Khasruzzaman, Md Tanvir Rahman, Muhammad Tofazzal Hossain, Md Alimul Islam","doi":"10.5455/javar.2023.j711","DOIUrl":"https://doi.org/10.5455/javar.2023.j711","url":null,"abstract":"<p><strong>Objective: </strong>Whole genome sequencing (WGS) of <i>Aeromonas veronii</i> Alim_AV_1000 isolated from ulcerative lesions of Shing fish (stringing catfish; <i>Heteropneustes fossilis</i>) was performed during the outbreak year 2021.</p><p><strong>Materials and methods: </strong>Using next-generation sequencing (Illumina) technology, WGS was accomplished, resulting in the sequencing, assembly, and analysis of the entire genome of the <i>A. veronii</i> strain. Moreover, the genomic features, virulence factors, antimicrobial resistome, and phylogenetic analysis for the molecular evolution of this strain were also examined.</p><p><strong>Results: </strong>The genome size of the <i>A. veronii</i> Alim_AV_1000 strain was 4,494,515 bp, with an average G+C content of 58.87%. Annotation revealed the known transporters and genes linked to virulence, drug targets, and antimicrobial resistance.</p><p><strong>Conclusion: </strong>The findings of the phylogenetic analysis revealed that the strain of the present study has a close relationship with the China strain TH0426 and strain B56. This study provides novel information on <i>A. veronii</i> isolated from Shing fish in Bangladesh.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134648964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and characterization of quinolone-resistant <i>Escherichia coli</i> isolated from retail raw beef and poultry meat in Egypt.","authors":"Dina Ibrahim, Amal Awad, Gamal Younis","doi":"10.5455/javar.2023.j702","DOIUrl":"https://doi.org/10.5455/javar.2023.j702","url":null,"abstract":"<p><strong>Objective: </strong>The goal of this study was to look at quinolone-resistant (QR) <i>Escherichia coli</i> (<i>E. coli</i>) from retail beef and poultry meat in Egypt by looking at the QR mechanisms in the resistant strains.</p><p><strong>Materials and methods: </strong>In total, 120 samples of raw poultry meat (<i>n</i> = 60) and beef meat (<i>n</i> = 60) were purchased from Mansoura retail stores between January and March 2021, and evaluated microbiologically for <i>E. coli</i>. Then, an antimicrobial sensitivity test was applied to all isolates. The prevalence of QR <i>E. coli</i> with concern for the QR determinants, including quinolone resistance-determining regions (QRDRs) mutations, the plasmid-mediated quinolone resistance gene (PMQR), and the efflux pump activity were determined.</p><p><strong>Results: </strong>The total prevalence of <i>E. coli</i> was 34.2% (41/120). Noticeably, the prevalence of <i>E. coli</i> in poultry meat (40%, 24/60) was higher than that of beef (28%, 17/60). All strains were assessed for their antimicrobial susceptibility using the disc diffusion technique; the highest rate of resistance (100%) was displayed to clindamycin and cefuroxime, followed by ampicillin (97.6%), doxycycline (92.7%), amoxicillin-clavulanate (92.7%), nalidixic acid (NA) (80.5%), sulfamethoxazole/trimethoprim (70.7%), chloramphenicol (63.4%), gentamicin, and azithromycin (58.5% each). Multiple antimicrobial resistance (strains resistant to three or more antimicrobial classes) was displayed by 97.6% of <i>E. coli</i> isolates. Regarding QR, 37 isolates could resist at least one of the examined quinolones. Regarding PMQR genes, <i>qnrS</i> was determined in 70% (7/10) of QR <i>E. coli,</i> while <i>qnr</i>A, <i>qnrB,</i> and <i>qnr</i>D were not identified. While the mutations determined regions of QR in the resistant <i>E. coli</i> isolates, S83L was the most prevalent in gyrase subunit A either alone or combined with D87N and D87Y, and three isolates of QR <i>E. coli</i> isolates revealed a <i>topoisomerase IV subunit</i> mutation harboring S80I. 20% of the isolates displayed efflux activity, as NA showed a considerable difference between its zones of inhibition.</p><p><strong>Conclusion: </strong>The high prevalence of antimicrobial-resistant <i>E. coli,</i> with concern for QR strains harboring different resistance mechanisms in poultry meat and beef, threatens the public's health. Thus, standard manufacturing procedures and adequate hygiene conditions must be followed in all phases of meat preparation, production, and consumption, and public knowledge should be improved.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134648968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in probiotication of fruit and vegetable juices.","authors":"Md Saydar Rahman, Dwip Das Emon, Maria Afroz Toma, Asmaul Husna Nupur, Poly Karmoker, Abdullah Iqbal, Mohammad Gulzarul Aziz, Md Abdul Alim","doi":"10.5455/javar.2023.j706","DOIUrl":"https://doi.org/10.5455/javar.2023.j706","url":null,"abstract":"<p><p>Probiotics are live bacteria beneficial to health when consumed adequately. Health professionals now recommend probiotics on regular diets due to their positive effects on human health. The probiotics that are usually consumed from the market through food products are mostly dairy-based. Fruit and vegetables are gaining popularity as preferred matrices for probiotic carriers to the human body, owing to their high cholesterol content and the lactose intolerance of dairy products. On the other hand, fruits and vegetable juices are rich in nutrient content such as vitamins, minerals, and antioxidants and do not contain a starter culture that can compete with the nutrients. The probiotication of fruit and vegetable juices (apple, carrot, citrus fruit, pome-granate, watermelon, tomato, and pineapple) are performing as efficient probiotic bacteria carriers. This review covers the previous works that highlighted the variety of probiotic fruit and vegetable juices as well as the viability of each probiotic in various products after proper fermentation and storage. In addition, physicochemical and sensory changes that occurred during the processing and storage period have been discussed. Furthermore, strategies (microencapsulation, adding prebiotics, antioxidant addition, maintaining optimum pH, temperature, adaptation with resistance, and good packaging) to improve the stability of probiotic bacteria are outlined, as it is difficult to maintain the stability of probiotic bacteria during storage. Finally, the manuscript discusses the effect of probiotic fruit and vegetable juices on human health.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134648973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the influence of xanthan on mozzarella cheese characteristics focusing on its antimicrobial effect.","authors":"Ayah B Abdel-Salam, Mena Saad, Rania F Ahmed, Neveen S M Soliman","doi":"10.5455/javar.2023.j700","DOIUrl":"https://doi.org/10.5455/javar.2023.j700","url":null,"abstract":"<p><strong>Objective: </strong>This study was designed to show the effect of adding different levels of microbial (lab-produced) and commercial xanthan (CX) for 30 days on the sensory, chemical, and microbiological parameters of mozzarella cheese (MC).</p><p><strong>Materials and methods: </strong>The production of xanthan was done in Garcia-Ochoa's medium. The sensory evaluation of the examined MC was achieved through a tabulated scorecard<i>.</i> The Gerber method was used for the determination of MC fat%. The mean counts of staphylococci [colony forming unit (CFU)/gm], coliforms (most probable number/gm), fungi (CFU/gm), and mesophilic bacteria (CFU/gm) were estimated in different fortified cheeses. Also, mean counts of <i>Escherichia coli</i> O157 and <i>Staphylococcus aureus</i> in artificially contaminated MC were determined.</p><p><strong>Results: </strong>The microbial xanthan (MX) had a significant (<i>p</i> < 0.05) effect on the sensory parameters of the examined samples with its concentration (0.0007%) after 20 days of storage. The MX (0.0005%) and CX (0.0002%) had a significant effect on moisture, fat in dry matter, and protein percentage of MC throughout the storage period. The high meltability degree of MC was observed in samples with both types of xanthan (0.0002%) at the end of storage.</p><p><strong>Conclusion: </strong>Both types of xanthan at all concentrations had a significant reducing effect on <i>E. coli</i> O157 and <i>S. aureus</i> in all samples from 10 to 30 days of storage. Xanthan has accepted attentiveness and offers beneficial and safe characteristics that improve its adaptability in MC. In the Middle East, this survival trial of <i>E. coli</i> O157 and <i>S. aureus</i> in the MC supplemented by xanthan is considered a scarce exploratory investigation.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134649026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfilariae infection by <i>Acanthocheilonema reconditum</i> and <i>Dirofilaria immitis</i> and their molecular detection in a dog with lymphoma: Case report.","authors":"Rodrigo Lugo-Vargas, Ruben Dario Perez-Ramirez, Nicolas Carrillo-Godoy, Iang Schroniltgen Rondón-Barragán","doi":"10.5455/javar.2023.j701","DOIUrl":"https://doi.org/10.5455/javar.2023.j701","url":null,"abstract":"<p><strong>Objective: </strong>Microfilariae parasites are common in tropical regions, and some species are reported as potentially zoonotic. The diagnosis of filarial infection in dogs by cytology or hematologic techniques showed lower sensibility and specificity, which may result in misdiagnosis. Thus, molecular techniques seem to be an alternative to identifying and detecting microfilariae infections. On the other hand, lymphoma is one of the main tumors in domestic animals, with a high prevalence in domestic canines. This study aims to report a mixed infection with microfilariae in a dog with lymphoma, emphasizing its diagnosis and the possible role of this infection in the development of the neoplasia.</p><p><strong>Materials and methods: </strong>An 8-year-old male mixed breed dog was referred to consultation due to the presence of lethargy, recumbency, skin ulceration lesions, nonspecific pain manifestations, emesis, myoclonus in the left temporalis muscle, and seizures. Routine blood and biochemistry tests were normal, and cytology of the skin evidenced a microfilariae infection. The dog died due to a cardiorespiratory arrest, and tissue sampling was done for histopathology and molecular analysis at the necropsy examination.</p><p><strong>Results: </strong>Skin lesions were related to a microfilarial pyogranuloma related to <i>Acanthocheilonema reconditum</i>. Histopathology of the spleen and liver revealed a diffuse lymphoma composed of blast cells and large lymphocytes, distributed diffusely in the parenchyma and surrounding the vasculature. In the skin, microfilariae were seen in some superficial capillaries.</p><p><strong>Conclusion: </strong>This study describes a microfilariae mixed infection with <i>A. reconditum</i> and <i>Dirofilaria immitis</i> in a dog with a lymphoma and its molecular detection. To the knowledge of the authors, this is the first report of a mixed microfilariae infection in a tumor of a dog and highlights the use of molecular techniques, i.e., polymerase chain reaction, for an accurate diagnosis.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636073/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134649028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}