Modification of cryoprotectants on sperm cryopreservation: A study of embryo development.

IF 1.5 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Advanced Veterinary and Animal Research Pub Date : 2024-08-26 eCollection Date: 2024-09-01 DOI:10.5455/javar.2024.k804

Manggiasih Dwiayu Larasati, Silvia Werdhy Lestari, Mulyoto Pangestu, Andon Hestiantoro, Gito Wasian

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引用次数: 0

Abstract

Objective: This research was conducted to analyze the effect of cryopreservation of sperm using modified cryoprotectants on embryo development through in vitro fertilization (IVF). In this research, three types of cryoprotectant combinations were compared, including Nakagata, modified cryoprotectant, and commercial (Kitazato).

Materials and methods: Several parameters, namely sperm concentration, motility, morphology, plasma membrane integrity, cryo-survival rate, and viability rate, were measured and compared before vitrification and warming. Embryo development was also observed on the first and third days of development based on the cell number, cell size, and fragmentation rate.

Results: Sperm cryopreservation exhibited a negative influence on embryo quality. Both Nakagata cryoprotectants and modified cryoprotectants attained good-quality embryos. In terms of embryonic development, this research revealed a modified cryoprotectant superior to Nakagata's cryoprotectant, although Kitazato was superior to the Nakagata cryoprotectant and modified cryoprotectant. Significant differences were found in the three cryoprotectants on observations on day 1 and day 3, all of them with p-value < 0.05.

Conclusion: Modified cryoprotectant was found to be better than Nakagata but less significant than Kitazato in terms of embryonic development quality. Therefore, modified cryoprotectants could be a better alternative compared to commercial (Kitazato) cryoprotectants for improving embryo quality.

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冷冻保护剂对精子冷冻的影响：胚胎发育研究

研究目的本研究旨在分析使用改良冷冻保护剂冷冻精子对体外受精（IVF）胚胎发育的影响。在这项研究中，对三种冷冻保护剂组合进行了比较，包括 Nakagata、改良冷冻保护剂和商业冷冻保护剂（Kitazato）：测量并比较了玻璃化和升温前的精子浓度、活力、形态、质膜完整性、低温存活率和存活率等参数。在胚胎发育的第一和第三天，还根据细胞数量、细胞大小和破碎率对胚胎发育情况进行了观察：结果：精子冷冻对胚胎质量有负面影响。Nakagata 低温保护剂和改良低温保护剂都能获得优质胚胎。在胚胎发育方面，本研究发现改良型冷冻保护剂优于中形冷冻保护剂，但北里冷冻保护剂优于中形冷冻保护剂和改良型冷冻保护剂。三种低温保护剂在第 1 天和第 3 天的观察结果均有显著差异，P 值均小于 0.05：就胚胎发育质量而言，改良型低温保护剂优于 Nakagata，但显著性低于 Kitazato。因此，改良型低温保护剂与商用（北里）低温保护剂相比，在提高胚胎质量方面可能是更好的替代品。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Advanced Veterinary and Animal Research Veterinary-Veterinary (all)

CiteScore

2.70

自引率

7.10%

发文量

审稿时长

6 weeks

期刊介绍： Journal of Advanced Veterinary and Animal Research (JAVAR) - is an open access, international, peer-reviewed, quarterly, highly-indexed scientific journal publishing original research findings and reviews on all aspects of veterinary and animal sciences. Basic and applied researches on- - Anatomy & histology - Animal health economics - Animal nutrition - Animal reproduction - Animal science - Antimicrobial resistance (AMR) - Biochemistry - Biotechnology - Dairy science - Epidemiology - Food hygiene and technology - Genetics and breeding - Immunology - Microbiology - Parasitology - Pathology - Pharmacology & toxicology - Physiology - Poultry science - Preventive veterinary medicine - Public health - Surgery & obstetrics - Veterinary extension studies - Wildlife & aquatic medicine - Zoo animal medicine.