Journal of Advanced Veterinary and Animal Research最新文献

{"title":"Isolation and antimicrobial resistance patterns of <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> from caprine respiratory tract infections: A hospital-based clinical study.","authors":"Shanta Barua, Md Abu Sayeed, Md Ashiqur Rahman, Mohammad Mahmudul Hassan, Mohammed Yousuf Elahi Chowdhury, Eaftekhar Ahmed Rana","doi":"10.5455/javar.2024.k855","DOIUrl":"10.5455/javar.2024.k855","url":null,"abstract":"<p><strong>Objective: </strong><i>Staphylococcus aureus</i> and <i>Escherichia coli</i> are the most common opportunistic pathogens frequently associated with respiratory tract infection (RTI) in different animals. This cross-sectional study aimed to identify the occurrence of <i>S. aureus</i> and <i>E. coli</i> in goats with RTI, analyze the antimicrobial resistance patterns, and explore potential risk factors contributing to RTI.</p><p><strong>Materials and methods: </strong>A total of 120 nasal swab samples were collected from goats, and standard classical bacteriological methods were performed to isolate and identify <i>S. aureus</i> and <i>E. coli</i>. Subsequently, the disc diffusion method was employed to evaluate the antimicrobial sensitivity test. A logistic regression model was used to analyze the factors associated with RTI.</p><p><strong>Results: </strong>About 13.3% (<i>N =</i> 16; <i>N =</i> 120) isolates were confirmed as <i>S. aureus,</i> and 6.67% (<i>N =</i> 8; <i>N =</i> 120) isolates were confirmed as <i>E. coli</i>. All S. <i>aureus</i> isolates were resistant to ampicillin, and all <i>E. coli</i> isolates were resistant to amoxicillin and penicillin. Among the isolated organisms, 43.7% (<i>N =</i> 7; <i>N =</i> 16) <i>S. aureus</i> and 62.5% (<i>N =</i> 5; <i>N =</i> 8) <i>E. coli</i> isolates were found to be multidrug-resistant (resistant to ≥3 classes of antimicrobials). Multivariable logistic regression analysis revealed that female goats [(odds ratio) OR: 4.2; 95% confidence intervals (CI): 0.8-20.8; <i>p</i> = 0.074] and goats in poor health condition (OR: 3.8; 95% CI: 0.7-19.3; <i>p</i> = 0.100) were more prone to RTI caused by <i>S. aureus</i>. Besides, goats that were not dewormed (OR: 4.8; 95% CI: 1-23.6; <i>p</i> = 0.051) and those reared in semi-intensive conditions (OR: 2.7; 95% CI: 0.8-8.7; <i>p</i> = 0.092) were found to be at higher risk of <i>S. aureus</i>-mediated RTI.</p><p><strong>Conclusion: </strong>The findings highlight the importance of implementing improved farm management practices and efficient antimicrobial resistance control approaches to minimize respiratory infections and reduce the burden of antibiotic resistance in goats.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 4","pages":"1037-1050"},"PeriodicalIF":1.5,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855430/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of zinc sulfate for the development of zinc-fortified meat products from broiler meat.","authors":"Asma Sultana, Nathu Ram Sarker, Raihan Habib, Md Shahin Alam, Dulal Chandra Paul, Mst Farhana Sharmin, Aminul Islam, Ruhul Amin, Md Sazedul Karim Sarker","doi":"10.5455/javar.2024.k864","DOIUrl":"10.5455/javar.2024.k864","url":null,"abstract":"<p><strong>Objective: </strong>The study aimed to assess the feasibility of incorporating food-grade zinc sulfate (ZnSO₄) for making zinc-fortified meatballs from broiler meat.</p><p><strong>Materials and methods: </strong>A total of 20 broiler breasts were treated with different concentrations of elemental zinc (Zn) of food-grade ZnSO₄ for making meatballs. The study included a control group without added Zn (T0: 0 mg/kg) and treatment groups with Zn concentrations of 10 mg/kg (T1), 20 mg/kg (T2), 30 mg/kg (T3), and 40 mg/kg (T4). Post-cooking, the meatball samples underwent preservation at -20°C for proximate composition, quality parameters, storage quality, texture, and sensory analysis.</p><p><strong>Results: </strong>Though the moisture content of meatballs did not differ significantly (<i>p</i> > 0.05), the T4 meatballs showed higher moisture percentages and significantly (<i>p</i> < 0.01) higher crude protein content compared to the control. However, a significant increase in cook yield (<i>p</i> < 0.01) and water-holding capacity (<i>p</i> < 0.05) were revealed by the T4 group. The T4 meatball recovered around 41.8% Zn. The malondialdehyde levels in all samples were acceptable when kept in the chiller for ten days. Moreover, the addition of varying doses of ZnSO₄ did not result in a significant alteration (<i>p</i> > 0.05) in the hardness of the meatballs. The sensory panelists also validated the instrumental outcome of hardness.</p><p><strong>Conclusion: </strong>The addition of 40 mg/kg of elemental Zn from ZnSO₄ improves nutritional composition and quality with no alteration of the texture and sensory properties of the meatball. This dose could be an effective strategy for fortifying chicken meat products and could offer a valuable source of dietary Zn for human consumption.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 4","pages":"1130-1138"},"PeriodicalIF":1.5,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of quail egg supplements enriched with marine macroalgae <i>Eucheuma</i> spinosum on the physiological condition of Sprague Dawley rats during pregnancy.","authors":"Hasan Basri, Slamet Widiyanto, Hendry T S Saragih, Zuprizal Zuprizal","doi":"10.5455/javar.2024.k862","DOIUrl":"10.5455/javar.2024.k862","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect of quail egg supplements enriched with marine macroalgae <i>Eucheuma spinosum</i> on body weight and physiological conditions of <i>Sprague Dawley</i> rats during pregnancy.</p><p><strong>Materials and methods: </strong>This study used a completely randomized experimental design. The test animals were 25 pregnant white rats aged 3 months and weighed ± 200 gm. Pregnant rats were divided into five treatments and five repetitions; each repetition contained one pregnant rat. T0: control treatment; T1: treatment group consuming quail eggs from quail fed commercial feed; T2: treatment group consuming quail eggs from quail fed with 3% <i>E. spinosum</i>; T3: treatment group consuming quail eggs from quail fed with 4% <i>E. spinosum</i>; T4: treatment group consuming quail eggs from quail fed with 5% <i>E. spinosum</i>. The parameters measured were egg proximate, egg iron, egg cholesterol, red blood cell (RBC), hemoglobin (Hb), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular Hb (MCH), mean corpuscular Hb concentration (MCHC), white blood cell (WBC), lymphocytes (LYM), <i>neutrophils</i> (NEUT), RBC distribution width (RDW), platelet distribution width (PDW), mean platelet volume (MPV), cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), glucose, <i>superoxide dismutase</i> (SOD), and m<i>alondialdehyde</i> (MDA).</p><p><strong>Results: </strong>The addition of <i>E. spinosum</i> into quail feed at concentrations of 3%, 4%, and 5% did not show statistically significant differences (<i>p</i> > 0.05) in the proximate levels (water, ash, fat, protein, and carbohydrates) compared to the control. However, the analysis of iron in quail eggs indicated a significant increase (<i>p</i> < 0.05) in comparison to the control treatment. In addition, the supplementation of <i>E. spinosum</i> in the quail feed resulted in significantly reduced (<i>p</i> < 0.05) quail egg cholesterol levels when compared to the control treatment. Furthermore, the supplementation of quail egg produced by quail fed with <i>E. spinosum</i> did not significantly affect the birth weight of the pups, RBC, MCV, MCH, MCHC, WBC, LYM, NEUT, RDW, PDW, MPV, MDA, and SOD when compared to the control treatment (<i>p</i> > 0.05). Meanwhile, the mother weight, Hb, HCT, cholesterol, HDL, LDL, and glucose levels significantly increased (<i>p</i> < 0.05) compared to the control.</p><p><strong>Conclusion: </strong>The administration of <i>E. spinosum</i> with concentrations of 3%, 4%, and 5% can increase iron levels and decrease cholesterol levels in quail eggs. The administration of quail egg supplements produced by quail-fed additional macroalgae <i>E. spinosum</i> can increase body weight, Hb, and HCT in pregnant rats. The addition of marine macroalgae with concentrations of 4% and 5% can decrease the levels of cholesterol, LDL, and glucose and increase serum HDL levels in pregnant rats.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 4","pages":"1114-1121"},"PeriodicalIF":1.5,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transformation toward precision large-scale operations for sustainable farming: A review based on China's pig industry.","authors":"Md Kamrul Hasan, Hong-Seok Mun, Keiven Mark B Ampode, Eddiemar B Lagua, Hae-Rang Park, Young-Hwa Kim, Md Sharifuzzaman, Chul-Ju Yang","doi":"10.5455/javar.2024.k859","DOIUrl":"10.5455/javar.2024.k859","url":null,"abstract":"<p><p>This review evaluates the current situation of pig farming, identifies challenges, and projects for the sustainable development of the Chinese pig industry. A literature review using keyword searches was conducted on Google Scholar for articles from 2017-2023. The review included studies focused on pig farming in China, covering prospects, challenges, quantitative data on production, marketing, and consumption, automation in livestock farming, and publications from peer-reviewed journals, credible websites, government reports, and conference proceedings. Pork consumption in China is increasing, and the country imports a sizable amount of pork annually. Even though small-scale farms still account for most operations, the pig industry is undergoing a critical stage of modernization and transition towards large-scale farming. The major challenges identified were feed, disease, antimicrobial resistance, environmental pollution, and pork prices. Smart technologies, such as cameras, Internet of Things, and sensors, integrated into precision pig farming can improve productivity and animal health through real-time data collection and decision-making. To solve the problems we face now, we need to put a lot of money into large-scale transformation, the creation of new animal precision tools, the automation of manure treatment, and the research and development of long-lasting alternative energy sources like photovoltaics and wind. By implementing these strategies, large-scale precision pig farming in China can become economically and environmentally sustainable, which can ultimately benefit consumers by supplying wholesome pork products.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 4","pages":"1076-1092"},"PeriodicalIF":1.5,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855412/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic resistance and virulence profile of <i>Klebsiella pneumoniae</i> isolated from wild Sumatran Orangutans (<i>Pongo abelii</i>).","authors":"Usamah Afiff, Rahmat Hidayat, Agustin Indrawati, Titiek Sunartatie, Aprilia Hardiati, Dordia Anindita Rotinsulu, Raden Iis Arifiantini, Deandarla Naoremisa, Nurhashunatil Mar'ah, Safika Safika","doi":"10.5455/javar.2024.k858","DOIUrl":"10.5455/javar.2024.k858","url":null,"abstract":"<p><strong>Objective: </strong>Orangutans (<i>Pongo abelii</i>), as endemic primates of Indonesia, are characterized by a predominantly arboreal lifestyle. <i>Klebsiella pneumoniae</i> (<i>K. pneumonia</i>) and other Gram-negative bacteria are present in the Indigenous flora of many mammals, including orangutans. This study aimed to investigate the antibiotic resistance and virulence profile of <i>K. pneumonia</i> isolated from wild Sumatran orangutans.</p><p><strong>Materials and methods: </strong>This study investigated 10 fecal samples from wild Sumatran orangutans from the Gunung Leuser National Park, Aceh, Indonesia. Biochemical and molecular identification of <i>K. pneumoniae</i> using the RNA polymerase subunit b gene and detection of virulence-associated genes. In addition, molecular detection of antibiotic resistance genes was performed to characterize the resistance mechanisms in the isolates.</p><p><strong>Results: </strong><i>K. pneumonia</i> was detected in 6 out of 10 fecal samples from wild Sumatran orangutans. The virulence genes <i>mrk</i>D and <i>ent</i>B were detected in all (100%) of the isolates, whereas wabG was identified in 83.33% of the strains. Antibiotic susceptibility testing against <i>K. pneumoniae</i> revealed that three isolates were susceptible to streptomycin (S) and nalidixic acid (NA), while all six isolates were susceptible to chloramphenicol and ciprofloxacin. One isolate demonstrated intermediate resistance to NA, while the remaining two exhibited intermediate resistance to S. Six isolates were resistant to ampicillin, tetracycline, and erythromycin, indicating multidrug resistance. Furthermore, antibiotic resistance genes were detected in the isolates with the following prevalence: <i>bla</i> _TEM gene (six isolates; 100%), <i>bla</i> _SHV (six isolates; 100%), <i>bla</i> _CTX-M gene (four isolates; 66.67%), and <i>tet</i>A gene (four isolates; 66.67%).</p><p><strong>Conclusion: </strong>This study revealed the virulence and resistance profile of <i>K. pneumoniae</i> bacterium isolated from wild Sumatran orangutans, which is essential for formulating effective conservation and healthcare strategies.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 4","pages":"1066-1075"},"PeriodicalIF":1.5,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the skin blackening phenomenon in Youzhou Dark goats based on the histological characteristics of melanocytes.","authors":"Cancan Chen, Jie Li, Xiaoyan Sun, Jing Jiang, Shipeng Lv, Liangjia Liu, Gaofu Wang, Hangxing Ren","doi":"10.5455/javar.2024.k865","DOIUrl":"10.5455/javar.2024.k865","url":null,"abstract":"<p><strong>Objective: </strong>The study was conducted to identify the molecular mechanism of the phenotype formation of Youzhou black sheep by histological cytology and transcriptomics.</p><p><strong>Materials and methods: </strong>In this study, HE and IHC staining were used to explore the patterns and cytological differences in skin pigment deposition between Youzhou Dark goats and Banjiao goats. In addition, the DEGs related to the black skin phenotype were identified via transcriptomic analyses. Finally, the expression pattern of the agouti signal protein (ASIP) gene in the skin from individuals with different skin color phenotypes was verified by FISH.</p><p><strong>Results: </strong>The results showed that compared with that on the skin surface of Banjiao goats, melanin deposition on the skin surface of Youzhou Dark goats was abnormally increased. The transcriptomic analyses showed that the expression of the <i>ASIP</i> genes decreased significantly in Youzhou Dark goats. FISH confirmed that the expression of the <i>ASIP</i> gene in Youzhou Dark goats was significantly lower than that in Banjiao goats.</p><p><strong>Conclusion: </strong>The present study showed that a decrease in <i>ASIP</i> gene expression and an increase in melanin production are important factors associated with skin pigmentation in Youzhou Dark goats.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 4","pages":"1139-1148"},"PeriodicalIF":1.5,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection of a novel mutation in the <i>TPO</i> gene associated with congenital hypothyroidism in a cat: Case report.","authors":"César Gallego-Munevar, Nicolas Carrillo-Godoy, Iang Schroniltgen Rondón-Barragán","doi":"10.5455/javar.2024.k854","DOIUrl":"10.5455/javar.2024.k854","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to analyze the sequence of different fragments of the thyroperoxidase (TPO) gene from a cat diagnosed with congenital hypothyroidism (CH).</p><p><strong>Materials and methods: </strong>The feline was diagnosed due to high serum concentrations of thyroid-stimulating hormone and low T4. The analysis of sequences containing mutations in the TPO gene from dogs with CH allowed for the prediction of mutation sites within the gene in an affected cat. In addition, the design of a polymerase chain reaction-based test allowed the amplification and sequencing of these gene segments. In addition, after the death of the patient, a necropsy and histopathology were performed, looking for macroscopic and microscopic alterations of affected organs.</p><p><strong>Results: </strong>The necropsy examination showed megacolon, cardiac concentric left ventricular hypertrophy, and bilateral enlargement of the thyroid gland. The histopathology of the thyroid showed follicular hypoplasia and low colloid production. gDNA analysis allowed the detection of mutation in the <i>TPO</i> gene, which corresponded to one transition in the nucleotide 12.542 (A > G) and heterozygous variations located in the nucleotide 14.627 (G/A) and in the nucleotide 30.713 (G/C).</p><p><strong>Conclusion: </strong>Due to the presence of these polymorphisms, it is suspected that one monoallelic expression of mutant alleles is present. More studies that allow an understanding of the role of the heterozygous in this pathology are required, as well as the role of gene mutations related to CH in cats. On the other hand, the data from the present study serve as the base for the development of a molecular test that allows a fast and accurate diagnosis of HC in cats.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 4","pages":"1030-1036"},"PeriodicalIF":1.5,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of melatonin, zinc sulfate, nano-melatonin, and nano-zinc oxide on mitochondria function and developmental competence of buffalo oocytes.","authors":"Omaima Mohamed Kandil, Heba Elsaeed Elsehy, Nabil Mohamed Baker, Mohamed Asran Elbehiry, Sayed Ahmed Hattab","doi":"10.5455/javar.2024.k860","DOIUrl":"10.5455/javar.2024.k860","url":null,"abstract":"<p><strong>Objective: </strong>The aim of the current work is studying the effect of antioxidants and nano--antioxidants on <i>in vitro</i> development and mitochondrial function of buffalo oocytes.</p><p><strong>Materials and methods: </strong>Good and excellent Buffalo oocytes were <i>in vitro</i> matured: (1) tissue culture medium-199 (control group), (2) TCM-199 + melatonin (Mel) 10^-9 M (Mel group), (3) TCM-199 + zinc 10^-6 M (Zn group), (4) TCM-199 + nano- Mel 10^-6 M (N-Mel group), and (5) TCM-199 + nano-zinc-oxide 10^-6 M (N-ZnO group) and incubated with CO₂ 5% and 38.5°C for 22 hr. <i>In vitro</i>-matured oocytes were either stained for mitochondrial function or cultured for detection of embryo development.</p><p><strong>Results: </strong>The maturation rate of buffalo oocytes in the N-Mel and N-ZnO groups had a significant (<i>p</i> < 0.05) increase (91.89% and 93.64%, respectively) compared to the Mel group (85.78%) and Zn group (81.37%), and all groups were significantly higher than the control (73.16%). Mitochondrial intensity was significantly elevated (<i>p</i> < 0.05) in the N-Mel and N-ZnO groups than in oocytes matured in the Mel, Zn, or control groups. Rates of fertilization, cleavage, and transferable embryos of buffalo oocytes matured <i>in vitro</i> were significantly raised in the N-ZnO group (88.35%, 85.93%, and 30.71%, respectively) and the N-Mel group (86.74%, 82.75%, and 28.32%, respectively) (<i>p</i> < 0.05) when compared with the Mel group (82.46%, 77.25%, and 21.29%, respectively) and the Zn group (79.98%, 75.19%, and 19.68%, respectively), and all were increased significantly (<i>p</i> < 0.05) compared to the control group (71.76%, 68.7%, and 11.98%, respectively).</p><p><strong>Conclusion: </strong>Supplementation of maturation medium with Mel 10^-9 M and zinc sulfate 10^-6 M and nano-Mel 10^-6 M and nano-zinc oxide 10^-6 M improves buffalo oocyte maturation rates, mitochondrial function, and embryo development.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 4","pages":"1093-1104"},"PeriodicalIF":1.5,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of narrowband ultraviolet B (311 nm) exposure on skin carcinogenesis in Wistar rats.","authors":"Roro Inge Ade Krisanti, Septelia Inawati Wanandi, Puspita Eka Wuyung, Aida S D Hoemardani","doi":"10.5455/javar.2024.k861","DOIUrl":"10.5455/javar.2024.k861","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study is to determine narrowband UVB (NB-UVB) irradiation's effect on the promotion of skin cancer, particularly its effect on DNA damage, oxidative stress, inflammation, and histological changes in Wistar rat skin.</p><p><strong>Materials and methods: </strong>Wistar rats were selected for this study and randomly divided into control, dimethylbenzanthracene (DMBA), and DMBA+NB-UVB groups. The rats were given a single dose of DMBA and exposed to NB-UVB 3 times a week for 10 weeks. The radiation dose started with 1 minimal erythema dose, which is equivalent to 3.192 J/cm². In the 11th week, analysis on cyclobutene pyrimidine dimer (CPD), malondialdehyde (MDA), nuclear factor kappa-B (NFκB), inflammatory cytokines, and histopathology examination of the skin tissue was conducted.</p><p><strong>Results: </strong>Higher CPD, MDA, NFκB, tumor necrosis factor a (TNF-a), interleukin (IL)-6, IL-11, IL-10, and IL-12 levels in rats exposed to DMBA+NB-UVB for 10 weeks compared to control and DMBA groups. Macroscopic examination presented erythema, skin thickening, desquamation, ulcer, and crust. Histopathology examination showed hyperkeratosis, acanthosis, atypical keratinocytes, irregular arrangement of the basement membrane, and inflammatory cell infiltration in the DMBA+NB-UVB group.</p><p><strong>Conclusion: </strong>This research has shown that 10 weeks of a combination of DMBA and NB-UVB irradiation induced DNA damage, oxidative stress, inflammation, and histological changes in the Wistar rat skin.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 4","pages":"1105-1113"},"PeriodicalIF":1.5,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of edible bird's nest and EDTA on cadmium toxicity exposed rats' embryo production, quality, and pre- and post-embryo transfer pregnancy rates.","authors":"Anmar Jasim Mohammed, Nurhusien Yimer, Faez Firdaus Abdullah Jesse, Wan Nor Fitri Wan Jaafar, Ainu Husna","doi":"10.5455/javar.2024.k844","DOIUrl":"10.5455/javar.2024.k844","url":null,"abstract":"<p><strong>Objectives: </strong>The current study aimed to investigate the prophylactic potential of EBN compared to EDTA in mitigating Cd's toxic effects on pregnancy rates and embryonic development in rats.</p><p><strong>Materials and methods: </strong>Ninety-eight female rats (Sprague Dawley) were divided into donor and recipient groups, with donors further divided into seven subgroups, including negative control, Cd-exposed, EBN-treated, and EDTA-treated groups. Embryos from donors were transferred to recipient rats, with EBN and Cd administered for 4 weeks and EDTA given only in the last 5 days for the donor group.</p><p><strong>Results: </strong>Results showed significant differences in pregnancy rates and blastocyst quality. EBN at 120 mg/kg BW led to higher blastocyst production and better quality compared to Cd-exposed groups. The highest pregnancy rates in recipient groups correlated with the highest blastocyst scores from donors.</p><p><strong>Conclusion: </strong>EBN at 120 mg/kg demonstrated significant protection against Cd toxicity and its effect on pregnancy rates, embryo production, quality, and pre- and post-embryo transfer, surpassing the effects of both 90 mg/kg EBN and EDTA. This study provides empirical evidence in support of the conventional belief in the positive impact of EBN on female reproduction.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 4","pages":"944-953"},"PeriodicalIF":1.5,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855418/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}