Journal of Advanced Veterinary and Animal Research最新文献

{"title":"Hepatoprotective, anti-inflammatory, and wound healing effects of spirulina in rats.","authors":"Md Imran Hossain, Sabbya Sachi, Purba Islam, Popy Khatun, Kazi Rafiq, Md Zahorul Islam, Atsushi Miyamoto","doi":"10.5455/javar.2025.l897","DOIUrl":"10.5455/javar.2025.l897","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to investigate the hepatoprotective, anti-inflammatory, and wound-healing potentials of spirulina utilizing a rat model.</p><p><strong>Materials and methods: </strong>Carbon tetrachloride (CCl₄) was used to induce hepatotoxicity, while carrageenan was employed to induce hind paw inflammation. The wound healing capability was assessed by making a 6 mm round wound with a biopsy punch on the dorsal interscapular area of each rat. The animals were fed a diet mixed with spirulina at dosages of 250 and 500 mg.kg^-1bwt. Paw thickness measurements were taken at 1, 3, and 6 h after carrageenan injection.</p><p><strong>Results: </strong>Intraperitoneal injection of CCl₄ leads to hepatotoxicity, as evidenced by significantly elevated serum levels of alanine aminotransferase and aspartate aminotransferase. Treatment with spirulina markedly reduced these biochemical markers. CCl₄-induced hepatic cellular necrosis, central vein congestion, and steatosis were notably improved following spirulina administration. Additionally, spirulina treatment diminished paw edema and shortened wound closure time in a dose-dependent manner. Histopathological analysis of the inflamed paw revealed massive infiltration of inflammatory cells and thickening of the epidermis, both of which showed significant improvement with spirulina treatment. The anti-inflammatory and wound-healing effects of spirulina were comparable to those of indomethacin, an established anti-inflammatory drug.</p><p><strong>Conclusion: </strong>Our findings demonstrate that <i>Spirulina platensis</i> possesses beneficial properties in counteracting hepatotoxicity and inflammation. Additionally, it exhibited significant wound-healing effects in rat models. These results reinforce the potential health advantages of spirulina as an effective functional food.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 1","pages":"303-311"},"PeriodicalIF":1.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144496702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Chromobacterium violaceum</i> isolated from pleural effusion in cat and antimicrobial susceptibility profile: A rare case report.","authors":"Neeranoot Detcharoenyos, Somyod Kankuntod, Suksan Chumsing, Monticha Suwunwong, Piyaporn Eiamcharoen, Amornthep Archawakulathep","doi":"10.5455/javar.2025.l896","DOIUrl":"10.5455/javar.2025.l896","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to present <i>Chromobacterium violaceum</i> isolated from a pleural effusion in an indoor-outdoor cat.</p><p><strong>Materials and methods: </strong>A 3-year-old male domestic shorthair cat was brought to the hospital with dyspnea. The thoracic radiographs and ultrasound showed the presence of a pleural effusion. The thoracic fluid was sent for cytological analysis, reverse transcription polymerase chain reaction (RT-PCR) for feline infectious peritonitis (FIP), and bacterial culture.</p><p><strong>Results: </strong>Cytological findings illustrated the protein-rich modified transudate. Albumin: globulin ratio was 0.4. RT-PCR testing for FIP was negative. The bacterial culture presented a pure growth of violet-pigmented colonies on both sheep blood agar and MacConkey agar. The isolation was identified and further confirmed as <i>C</i>. <i>violaceum</i>. The isolation was susceptible to drugs in aminoglycosides, tetracyclines, macrolides, fluoroquinolones, and sulfonamide groups.</p><p><strong>Conclusion: </strong><i>Chromobacterium violaceum</i>, a saprophytic Gram-negative bacterium, commonly inhabits stagnant water and soil in tropical and subtropical areas. It is considered an opportunistic bacterium in both veterinary and human medicine. Although <i>C</i>. <i>violaceum</i> infection is rare, the disease is extremely fatal. In Thailand, a few cases of humans infected with <i>C</i>. <i>violaceum</i> were reported; however, the bacterial infection has never been discovered in animals. This case report highlighted a rare opportunistic infection of <i>C</i>. <i>violaceum</i>in a cat in Thailand and suggested drugs of choice for clinical treatments.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 1","pages":"297-302"},"PeriodicalIF":1.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144496737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and molecular identification of the cat flea <i>Ctenocephalides felis</i> from Bangladesh.","authors":"Md Shamsudduha, Md Mahfuzur Rahman, Jannatun Naher, Azizul Islam Barkat, Sumaiya Akter, Mohammad Shamimul Alam","doi":"10.5455/javar.2025.l894","DOIUrl":"10.5455/javar.2025.l894","url":null,"abstract":"<p><strong>Objective: </strong>The present study was designed to conduct molecular and morphological identification of cat fleas (<i>Ctenocephalides felis</i>) from Bangladesh along with nucleotide polymorphism and phylogenetic analysis.</p><p><strong>Materials and methods: </strong>Samples were collected from two hosts (cat and human). The species was identified through morphological studies first, and then DNA was extracted for subsequent molecular analysis. A part of the mitochondrial <i>16S rRNA</i> gene was amplified by polymerase chain reaction using extracted DNA as a template. The amplified region was sequenced using the Sanger dideoxy method. The sequence was subjected to NCBI BLASTn search. BioEdit and MEGA 11 software were used for multiple sequence alignment (MSA) and generating a phylogenetic tree.</p><p><strong>Results: </strong>Morphological features such as shape, size, and appendages showed similarity with <i>C. felis.</i> The metatibial formula of chaetotaxy (2-2-2-2-1-3) was confirmed for species-level identification. NCBI BLASTn search showed the highest sequence identity with the available sequence of <i>C</i>. <i>felis</i> such as 99.78% (NC_049858.1) and 99.12% (MW420044.1 and MK941844.1) with 100% query coverage. MSA of <i>C</i>. <i>felis</i> sequences from different geographical distributions show their sequence affinities with each other, and the phylogenetic tree presents their relationship with each other.</p><p><strong>Conclusion: </strong>Both morphological and molecular studies clearly indicate the identity and confirmation of cat flea (<i>C</i>. <i>felis)</i> from Bangladesh.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 1","pages":"280-286"},"PeriodicalIF":1.5,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144496750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphometry, hematology, and plasma chemistry of common coot (<i>Fulica atra</i>) in Punjab, Pakistan.","authors":"Shozab Seemab Khan, Tariq Javed, Muhammad Saleem Khan, Zahid Farooq, Muhammad Wajid","doi":"10.5455/javar.2025.l898","DOIUrl":"10.5455/javar.2025.l898","url":null,"abstract":"<p><strong>Objective: </strong>The common coot (<i>Fulica atra</i>) is a medium-sized migratory bird wintering at different wetlands in Pakistan. It belongs to the order Gruiformes and family Rallidae. This study aimed to investigate the morphometry, hematology, and blood chemistry of common coots during the winter visit of 2022-2023.</p><p><strong>Materials and methods: </strong>Thirty two adult common coots were captured with the help of hunters holding valid licenses from six wetlands in Punjab, Pakistan. Each individual was sexed and subjected to different morphological measurements. Eighteen blood samples (3-5 ml) were collected from the basilic veins of adults for hematology and blood chemistry.</p><p><strong>Results: </strong>Results revealed that body weight ranged from 378 to 680 gm, with males significantly heavier than females. Length was not significantly different. Males showed dominance in most of the morphometric characteristics compared to females. Regarding hematological parameters and plasma chemistry, there was a sufficient difference between the genders in most of the studied parameters.</p><p><strong>Conclusion: </strong>The male coot was significantly larger compared to the female, except for total length. Hematology and plasma chemistry showed significant differences between genders.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 1","pages":"312-320"},"PeriodicalIF":1.5,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144496762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of the glucose and trehalose addition on the extenders for goat sperm liquid storage.","authors":"Md Emtiaj Alam, Most Shorifa Yeasmin, Dipak Kumar Das, Md Shohidul Islam, Soshe Ahmed, Md Hamidul Islam, Md Akhtarul Islam, Md Mostofa Kamal, Aurangazeb Kabir, Alam Khan, Md Hakimul Haque, Md Abdul Masum, Moizur Rahman, Mst Ishrat Zerin Moni","doi":"10.5455/javar.2025.l895","DOIUrl":"10.5455/javar.2025.l895","url":null,"abstract":"<p><strong>Objectives: </strong>The objective of this study was to assess the effects of varying glucose and trehalose concentrations on tris-citric acid-egg yolk-fructose (TCEF) diluents for the short-term cold storage of goat semen.</p><p><strong>Materials and methods: </strong>The semen sample was collected, unwashed, and divided into the following groups: control (TCEF without glucose and trehalose), TCEF + glucose (75, 150 mm), and TCEF + trehalose (75, 150 mm). Each experimental sperm group (sperm concentration: 9 × ×10⁷/ml) was kept at 4°C in a refrigerator. The impact of varying glucose and trehalose levels on the quality of the spermatozoa was assessed at different time points: after dilution and at 5, 24, 48, and 72 h of refrigeration.</p><p><strong>Results: </strong>After dilution, progressive motility (PM), total motility (TM), sperm viability (SV), functional integrity (FI), and acrosome integrity of G-75, G-150, T-75, and T-150 did not differ significantly from the control. The PM, TM, SV, FI, and acrosome integrity of sperm of T-150 were considerably lower than the control, G-75, G-150, and T-75 after 5 and 24 h of cool storage. The T-75 group showed superior PM, TM, and FI after 48 h of cool storage, with noticeably greater values than the other groups.</p><p><strong>Conclusion: </strong>This study indicates that trehalose is a more favorable sugar than glucose for 48 h cool storage of buck semen, providing greater advantages in PM, TM, and PMI.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 1","pages":"287-296"},"PeriodicalIF":1.5,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186796/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144496741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The phenolic and flavonoid content and biological activity of Curcuma (<i>Curcuma xanthorrhiza</i>) fractions with different solvent polarities.","authors":"Ucop Haroen, Syafwan Syafwan, Kiki Kurniawan, Agus Budiansyah, Nilawati Widjaja, Saitul Fakhri","doi":"10.5455/javar.2025.l886","DOIUrl":"10.5455/javar.2025.l886","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to identify the impact of variations in solvent polarity on the solubility of secondary metabolite compounds, which were correlated as antioxidant and antibacterial agents for <i>Escherichia coli</i> (ATCC 11725), <i>Salmonella sp.</i> (ATCC 22504), <i>Staphylococcus aureus</i> (ATCC 11526), and <i>Bacillus subtilis</i> (ATCC 11626).</p><p><strong>Materials and methods: </strong>A total of 500 gm of Temulawak flour (<i>Curcuma xanthorrhiza</i>) was macerated gradually using the step gradient polarity (SGP) technique, where the solvents (methanol, ethyl acetate, and n-hexane) used had different levels of polarity. The extracting process of secondary metabolite components of Temulawak flour began with the use of non-polar solvents, semipolar solvents, and polar solvents, respectively. Each soaking process was completed for 5 × 24 h.</p><p><strong>Results: </strong>From the Temulawak extract with different levels of solvent polarity, 76.048 gm were obtained for the methanol fraction, 106.242 gm for the ethyl acetate fraction, and 154.575 gm for the n-hexane fraction. The results of the antioxidant activity test showed that the ethyl acetate fraction had the highest inhibitory value for antioxidant activity of 50% (IC50) 22.59 mg/l. The results of this research's <i>β</i>-carotene content were 0.0865 gm. Ethyl acetate fraction was known to have good activity from the antibacterial activity test. The inhibition zone of <i>E. coli</i> and <i>B. subtilis</i> bacteria was 11.5 ± 0.71 with a minimum concentration of 3.13 mg/ml. The inhibition zone of <i>S. aureus</i> was 10.5 ± 0.17 with a minimum inhibitory concentration (MIC) of 6.25 mg/ml, while the inhibition zone for <i>Salmonella</i> sp. was 8.125 ± 0.35 with a MIC of 37.50 mg/ml.</p><p><strong>Conclusion: </strong>All fractions have moderate antibacterial activity, yet the ability of the ethyl acetate fraction of Temulawak extract was higher than the methanol and n-hexane fractions.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 1","pages":"192-204"},"PeriodicalIF":1.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144496705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, screening, and molecular identification of endopytic fungus producing cellulose and cyanide degrading enzyme its application for waste cassava.","authors":"Yetti Marlida, Husmaini Husmaini, Ahadiyah Yuniza, Lili Anggraini, Wulansih Dwi Astuti, Ridho Kurniawan Rusli, Hera Dwi Triani, Gusri Yanti","doi":"10.5455/javar.2025.l884","DOIUrl":"10.5455/javar.2025.l884","url":null,"abstract":"<p><strong>Objective: </strong>This research aims to isolate, screen, and identify some candidates for endophytic fungus-producing cellulase and cyanidase.</p><p><strong>Materials and methods: </strong>Fungi were isolated from cassava leaves that had undergone surface sterilization. The fungal isolates were qualitatively tested for their ability to produce cellulase and cyanidase enzymes by adding carboxy methyl cellulose (CMC) and KCN to the media. Enzyme production was indicated by the formation of clear zones around the growing colonies. Isolates that tested positive for cellulase and cyanidase production underwent further quantitative screening to measure enzyme activity using a spectrophotometer at wavelengths of 540 nm and 400 nm, respectively. The isolates showing the highest cellulase and cyanidase activity were identified through 18S rRNA analysis using the Sanger DNA sequencing method.</p><p><strong>Results: </strong>The research obtained six pure isolates of endophytic fungus, namely Y1; Y2; Y3; Y4; Y5; and Y6. Four isolates had the ability to degrade CMC with a clear zone between 0.1 until 0.5 mm, and three isolates had the ability for KCN degrade. The highest activity for cellulase and cyanidase degrading enzymes was produced by isolate Y2. After molecular identification using 18S rRNA, isolate Y2 had 98.82% similarity to <i>Phomopsis</i> sp. 32PG/F.</p><p><strong>Conclusion: </strong>Six isolates of endophytic fungi were obtained, Y1; Y2; Y3; Y4; Y5; and Y6. Four isolate the ability of to degrade CMC and three isolate the ability for KCN degrade. Isolate Y2 is the isolate with the best activity for cellulase and cyanidase degrading enzymes, namely 2.99 U/ml and 2.19 U/ml. After molecular identification using 18S rRNA, isolate Y2 had 98.82% similarity to <i>Phomopsis</i> sp. 32PG/F.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 1","pages":"169-178"},"PeriodicalIF":1.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186803/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144496747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aging time influences fatty acid profiles and volatile compounds in cooked Thai native beef.","authors":"Watcharawit Meenongyai, Kunwadee Kaewka, Kannika Wongpanit, Piyamas Phongkaew, Pichad Khejornsart, Panuwat Khumpeerawat, Alexander Michael Stelzleni","doi":"10.5455/javar.2025.l885","DOIUrl":"10.5455/javar.2025.l885","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to assess the impact of aging time on the quality of meat, fatty acid profiles, and volatile compounds in cooked Thai native beef.</p><p><strong>Materials and methods: </strong>The experiment utilized a randomized complete block design, where the aging time (0, 7, 14, 21, and 28 days) served as the treatment and the longissimus thoracis et lumborum muscle from Thai native cattle (<i>n</i> = 4) was considered a block.</p><p><strong>Results: </strong>Meat color and Warner-Bratzler shear force values decreased as the aging time increased. Furthermore, the moisture content decreased while the protein and ash content increased with a longer aging time. The proportions of fatty acids showed significant differences with varying aging times. These fatty acids exhibited the highest proportions in the 14-day dry-aged beef samples. The levels of short-chain aldehydes (pentanal, heptanal, octanal, nonanal, decanal, and 2-nonenal) and alcohols (1-hexanol and 1-octanol) decreased as the aging time increased. However, there was an increase in long-chain aldehydes (tetradecanal, hexadecanal, heptadecanal, and octadecanal) with longer aging time. In addition, the levels of toluene, dodecane, tridecane, methyl-pyrazine, 2,5-dimethyl-pyrazine, trimethyl-pyrazine, and dimethyl trisulfide were higher in 14-day dry-aged beef compared to beef that had not been aged. Furthermore, a correlation was found between the number of the 6 significant fatty acids in the dry-aged beef samples and the 12 volatile compounds in cooked beef.</p><p><strong>Conclusion: </strong>Our results suggest that aging times significantly influence the fatty acid profiles of Thai native beef, which in turn are correlated with the characteristic volatile compounds.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 1","pages":"179-191"},"PeriodicalIF":1.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144496738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplastic pollution in the marine environment: Sources, impacts, and degradation.","authors":"Osamah A Ahmad, Mamdoh T Jamal, Hamdah S Almalki, Abeer H Alzahrani, Amirah S Alatawi, Md Fazlul Haque","doi":"10.5455/javar.2025.l893","DOIUrl":"10.5455/javar.2025.l893","url":null,"abstract":"<p><p>Microplastics (MPs) are tiny particles derived from plastic, and their high fragmentation poses a significant threat to the marine environment. MPs can originate from various sources, such as primary or secondary sources as well as internal or external sources. However, in marine environments, MPs mainly enter from external sources, such as plastic waste, debris from land-based activities, tourism, shipping, and atmospheric deposition. MP accumulation in marine ecosystems is also influenced by the use of plastic equipment, aquaculture feed, health products, and particular environmental conditions. Understanding the ecotoxicological effects of environmentally relevant concentrations of MPs on the health of marine organisms is crucial. The effects of MPs on the health of marine organisms are well reported by different studies, highlighting their interactions with a wide range of marine life, including plankton, invertebrates, crustaceans, corals, seagrass, fish, and even humans. Thus, MPs have been reported as a notorious pollutant because of their deleterious impacts on the morphology, physiology, immunology, behavior, reproduction, and heredity of marine organisms. Moreover, most MPs are slowly degradable, and degradation is dependent on various biotic and abiotic factors, resulting in continuous accumulation in the marine environment. This review provides an in-depth explanation of the sources of MPs, along with their impacts on marine organisms, ecosystems, aquaculture, and human health. In addition, it will discuss the degradation of MPs in the marine environment to explore the potential strategies for reducing the harmful impacts of MPs.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 1","pages":"260-279"},"PeriodicalIF":1.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144496749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphometric traits and identification of GH and IGF-1 gene polymorphisms in Napu buffalo (<i>Bubalus bubalis</i>) from Central Sulawesi, Indonesia.","authors":"Amirudin Dg Malewa, Rusdin Rusdin, Mardiah Mangun, Isyana Khaerunnisa, Dwi Lestari, Yulius Duma","doi":"10.5455/javar.2025.l887","DOIUrl":"10.5455/javar.2025.l887","url":null,"abstract":"<p><strong>Objective: </strong>The objectives of this study are to measure the body weight (BW) and morphometric parameters of Napu buffaloes, identify the growth hormone (GH) and insulin-like growth factor-1 (IGF-1) gene polymorphisms, and associate them with the BW and morphometric parameters of Napu buffaloes.</p><p><strong>Materials and methods: </strong>This study used 39 Napu buffaloes (9 males and 30 females). Morphometric measurements were performed using a measuring tape. The GH and IGF-1 gene diversity analysis was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and direct sequencing techniques.</p><p><strong>Results: </strong>The results showed that there is diversity in BW and body size of Napu buffaloes due to the influence of age and sex. The results of PCR-RFLP analysis of the GH|<i>AluI</i> gene showed 0% VV, 100% LL, and 0% VL genotypes. While the IGF-1|<i>SnaBI</i> gene shows the genotype AA 0%, BB 100%, and AB 0%. The sequencing results of the GH and IGF-1 genes in Napu buffaloes did not find mutations.</p><p><strong>Conclusion: </strong>Age and sex in Napu buffaloes may affect BW and body size. The GH and IGF-1 genes in Napu buffaloes are monomorphic, so no association can be made with the morphometric parameters of Napu buffaloes.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 1","pages":"205-213"},"PeriodicalIF":1.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144496761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}