Morphological and molecular identification of the cat flea Ctenocephalides felis from Bangladesh.

IF 1.5 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Advanced Veterinary and Animal Research Pub Date : 2025-03-26 eCollection Date: 2025-03-01 DOI:10.5455/javar.2025.l894

Md Shamsudduha, Md Mahfuzur Rahman, Jannatun Naher, Azizul Islam Barkat, Sumaiya Akter, Mohammad Shamimul Alam

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引用次数: 0

Abstract

Objective: The present study was designed to conduct molecular and morphological identification of cat fleas (Ctenocephalides felis) from Bangladesh along with nucleotide polymorphism and phylogenetic analysis.

Materials and methods: Samples were collected from two hosts (cat and human). The species was identified through morphological studies first, and then DNA was extracted for subsequent molecular analysis. A part of the mitochondrial 16S rRNA gene was amplified by polymerase chain reaction using extracted DNA as a template. The amplified region was sequenced using the Sanger dideoxy method. The sequence was subjected to NCBI BLASTn search. BioEdit and MEGA 11 software were used for multiple sequence alignment (MSA) and generating a phylogenetic tree.

Results: Morphological features such as shape, size, and appendages showed similarity with C. felis. The metatibial formula of chaetotaxy (2-2-2-2-1-3) was confirmed for species-level identification. NCBI BLASTn search showed the highest sequence identity with the available sequence of C. felis such as 99.78% (NC_049858.1) and 99.12% (MW420044.1 and MK941844.1) with 100% query coverage. MSA of C. felis sequences from different geographical distributions show their sequence affinities with each other, and the phylogenetic tree presents their relationship with each other.

Conclusion: Both morphological and molecular studies clearly indicate the identity and confirmation of cat flea (C. felis) from Bangladesh.

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孟加拉猫蚤的形态与分子鉴定。

目的：对孟加拉国猫蚤（Ctenocephalides felis）进行分子形态学鉴定、核苷酸多态性和系统发育分析。材料和方法：从猫和人两种宿主中采集样本。首先通过形态学研究对其进行鉴定，然后提取DNA进行分子分析。以提取的DNA为模板，采用聚合酶链反应扩增线粒体16S rRNA基因的一部分。用Sanger双脱氧法对扩增区进行测序。对序列进行NCBI BLASTn检索。使用BioEdit和MEGA 11软件进行多序列比对（MSA），生成系统发育树。结果：外形、大小、附属物等形态学特征与猫科动物相似。确定了毛分类学的代谢公式（2-2-2- 1-3），用于种水平的鉴定。NCBI BLASTn检索结果显示，与C. felis可用序列的一致性最高，分别为99.78% （NC_049858.1）和99.12% (MW420044.1和MK941844.1)，查询覆盖率为100%。不同地理分布的猫毛猴序列MSA具有序列亲和性，系统发育树显示了它们之间的关系。结论：形态学和分子学研究明确了孟加拉国猫蚤的身份和鉴定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Advanced Veterinary and Animal Research Veterinary-Veterinary (all)

CiteScore

2.70

自引率

7.10%

发文量

审稿时长

6 weeks

期刊介绍： Journal of Advanced Veterinary and Animal Research (JAVAR) - is an open access, international, peer-reviewed, quarterly, highly-indexed scientific journal publishing original research findings and reviews on all aspects of veterinary and animal sciences. Basic and applied researches on- - Anatomy & histology - Animal health economics - Animal nutrition - Animal reproduction - Animal science - Antimicrobial resistance (AMR) - Biochemistry - Biotechnology - Dairy science - Epidemiology - Food hygiene and technology - Genetics and breeding - Immunology - Microbiology - Parasitology - Pathology - Pharmacology & toxicology - Physiology - Poultry science - Preventive veterinary medicine - Public health - Surgery & obstetrics - Veterinary extension studies - Wildlife & aquatic medicine - Zoo animal medicine.