Morphometric traits and identification of GH and IGF-1 gene polymorphisms in Napu buffalo (Bubalus bubalis) from Central Sulawesi, Indonesia.

IF 1.5 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Advanced Veterinary and Animal Research Pub Date : 2025-03-25 eCollection Date: 2025-03-01 DOI:10.5455/javar.2025.l887

Amirudin Dg Malewa, Rusdin Rusdin, Mardiah Mangun, Isyana Khaerunnisa, Dwi Lestari, Yulius Duma

{"title":"Morphometric traits and identification of GH and IGF-1 gene polymorphisms in Napu buffalo (Bubalus bubalis) from Central Sulawesi, Indonesia.","authors":"Amirudin Dg Malewa, Rusdin Rusdin, Mardiah Mangun, Isyana Khaerunnisa, Dwi Lestari, Yulius Duma","doi":"10.5455/javar.2025.l887","DOIUrl":null,"url":null,"abstract":"Objective: The objectives of this study are to measure the body weight (BW) and morphometric parameters of Napu buffaloes, identify the growth hormone (GH) and insulin-like growth factor-1 (IGF-1) gene polymorphisms, and associate them with the BW and morphometric parameters of Napu buffaloes.Materials and methods: This study used 39 Napu buffaloes (9 males and 30 females). Morphometric measurements were performed using a measuring tape. The GH and IGF-1 gene diversity analysis was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and direct sequencing techniques.Results: The results showed that there is diversity in BW and body size of Napu buffaloes due to the influence of age and sex. The results of PCR-RFLP analysis of the GH|AluI gene showed 0% VV, 100% LL, and 0% VL genotypes. While the IGF-1|SnaBI gene shows the genotype AA 0%, BB 100%, and AB 0%. The sequencing results of the GH and IGF-1 genes in Napu buffaloes did not find mutations.Conclusion: Age and sex in Napu buffaloes may affect BW and body size. The GH and IGF-1 genes in Napu buffaloes are monomorphic, so no association can be made with the morphometric parameters of Napu buffaloes.","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 1","pages":"205-213"},"PeriodicalIF":1.5000,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186792/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Advanced Veterinary and Animal Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/javar.2025.l887","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/3/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: The objectives of this study are to measure the body weight (BW) and morphometric parameters of Napu buffaloes, identify the growth hormone (GH) and insulin-like growth factor-1 (IGF-1) gene polymorphisms, and associate them with the BW and morphometric parameters of Napu buffaloes.

Materials and methods: This study used 39 Napu buffaloes (9 males and 30 females). Morphometric measurements were performed using a measuring tape. The GH and IGF-1 gene diversity analysis was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and direct sequencing techniques.

Results: The results showed that there is diversity in BW and body size of Napu buffaloes due to the influence of age and sex. The results of PCR-RFLP analysis of the GH|AluI gene showed 0% VV, 100% LL, and 0% VL genotypes. While the IGF-1|SnaBI gene shows the genotype AA 0%, BB 100%, and AB 0%. The sequencing results of the GH and IGF-1 genes in Napu buffaloes did not find mutations.

Conclusion: Age and sex in Napu buffaloes may affect BW and body size. The GH and IGF-1 genes in Napu buffaloes are monomorphic, so no association can be made with the morphometric parameters of Napu buffaloes.

查看原文本刊更多论文

印度尼西亚中苏拉威西纳布水牛生长激素和IGF-1基因多态性及形态计量学特征

目的：测定纳普水牛的体重（BW）和形态参数，鉴定生长激素（GH）和胰岛素样生长因子-1 （IGF-1）基因多态性，并将其与纳普水牛体重和形态参数联系起来。材料与方法：以39头纳普水牛为研究对象，其中公水牛9头，母水牛30头。使用卷尺进行形态测量。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和直接测序技术对GH和IGF-1基因多样性进行分析。结果：纳普水牛的体重和体型受年龄和性别的影响存在差异。PCR-RFLP分析结果显示GH|AluI基因为0% VV、100% LL和0% VL基因型。而IGF-1|SnaBI基因显示的基因型为AA 0%， BB 100%, AB 0%。纳普水牛的生长激素和IGF-1基因测序结果未发现突变。结论：年龄和性别可能影响纳普水牛的体重和体型。纳普水牛的生长激素和IGF-1基因是单态的，因此与纳普水牛的形态计量参数没有关联。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Advanced Veterinary and Animal Research Veterinary-Veterinary (all)

CiteScore

2.70

自引率

7.10%

发文量

审稿时长

6 weeks

期刊介绍： Journal of Advanced Veterinary and Animal Research (JAVAR) - is an open access, international, peer-reviewed, quarterly, highly-indexed scientific journal publishing original research findings and reviews on all aspects of veterinary and animal sciences. Basic and applied researches on- - Anatomy & histology - Animal health economics - Animal nutrition - Animal reproduction - Animal science - Antimicrobial resistance (AMR) - Biochemistry - Biotechnology - Dairy science - Epidemiology - Food hygiene and technology - Genetics and breeding - Immunology - Microbiology - Parasitology - Pathology - Pharmacology & toxicology - Physiology - Poultry science - Preventive veterinary medicine - Public health - Surgery & obstetrics - Veterinary extension studies - Wildlife & aquatic medicine - Zoo animal medicine.