Comparative study of the glucose and trehalose addition on the extenders for goat sperm liquid storage.

IF 1.5 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Advanced Veterinary and Animal Research Pub Date : 2025-03-26 eCollection Date: 2025-03-01 DOI:10.5455/javar.2025.l895

Md Emtiaj Alam, Most Shorifa Yeasmin, Dipak Kumar Das, Md Shohidul Islam, Soshe Ahmed, Md Hamidul Islam, Md Akhtarul Islam, Md Mostofa Kamal, Aurangazeb Kabir, Alam Khan, Md Hakimul Haque, Md Abdul Masum, Moizur Rahman, Mst Ishrat Zerin Moni

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引用次数: 0

Abstract

Objectives: The objective of this study was to assess the effects of varying glucose and trehalose concentrations on tris-citric acid-egg yolk-fructose (TCEF) diluents for the short-term cold storage of goat semen.

Materials and methods: The semen sample was collected, unwashed, and divided into the following groups: control (TCEF without glucose and trehalose), TCEF + glucose (75, 150 mm), and TCEF + trehalose (75, 150 mm). Each experimental sperm group (sperm concentration: 9 × ×10⁷/ml) was kept at 4°C in a refrigerator. The impact of varying glucose and trehalose levels on the quality of the spermatozoa was assessed at different time points: after dilution and at 5, 24, 48, and 72 h of refrigeration.

Results: After dilution, progressive motility (PM), total motility (TM), sperm viability (SV), functional integrity (FI), and acrosome integrity of G-75, G-150, T-75, and T-150 did not differ significantly from the control. The PM, TM, SV, FI, and acrosome integrity of sperm of T-150 were considerably lower than the control, G-75, G-150, and T-75 after 5 and 24 h of cool storage. The T-75 group showed superior PM, TM, and FI after 48 h of cool storage, with noticeably greater values than the other groups.

Conclusion: This study indicates that trehalose is a more favorable sugar than glucose for 48 h cool storage of buck semen, providing greater advantages in PM, TM, and PMI.

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葡萄糖和海藻糖在山羊精子液体储存中添加的比较研究。

目的：本研究的目的是评估不同浓度的葡萄糖和海藻糖对三柠檬酸-蛋黄-果糖（TCEF）短期冷藏山羊精液稀释剂的影响。材料与方法：收集精液样本，不清洗，分为对照组（TCEF不含葡萄糖和海藻糖）、TCEF +葡萄糖（75、150 mm）和TCEF +海藻糖（75、150 mm）。每个实验精子组（精子浓度：9 × ×107/ml）在4℃的冰箱中保存。在不同时间点评估不同葡萄糖和海藻糖水平对精子质量的影响：稀释后，冷藏5、24、48和72小时。结果：稀释后，G-75、G-150、T-75和T-150的渐进运动力（PM）、总运动力（TM）、精子活力（SV）、功能完整性（FI）和顶体完整性与对照组无显著差异。冷藏5 h和24 h后，T-150精子的PM、TM、SV、FI和顶体完整性显著低于对照、G-75、G-150和T-75。冷藏48 h后，T-75组的PM、TM和FI均显著高于其他各组。结论：本研究表明海藻糖比葡萄糖更有利于雄鹿精液冷藏48 h，在PM、TM和PMI方面具有更大的优势。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Advanced Veterinary and Animal Research Veterinary-Veterinary (all)

CiteScore

2.70

自引率

7.10%

发文量

审稿时长

6 weeks

期刊介绍： Journal of Advanced Veterinary and Animal Research (JAVAR) - is an open access, international, peer-reviewed, quarterly, highly-indexed scientific journal publishing original research findings and reviews on all aspects of veterinary and animal sciences. Basic and applied researches on- - Anatomy & histology - Animal health economics - Animal nutrition - Animal reproduction - Animal science - Antimicrobial resistance (AMR) - Biochemistry - Biotechnology - Dairy science - Epidemiology - Food hygiene and technology - Genetics and breeding - Immunology - Microbiology - Parasitology - Pathology - Pharmacology & toxicology - Physiology - Poultry science - Preventive veterinary medicine - Public health - Surgery & obstetrics - Veterinary extension studies - Wildlife & aquatic medicine - Zoo animal medicine.