The phenolic and flavonoid content and biological activity of Curcuma (Curcuma xanthorrhiza) fractions with different solvent polarities.

IF 1.5 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Journal of Advanced Veterinary and Animal Research Pub Date : 2025-03-25 eCollection Date: 2025-03-01 DOI:10.5455/javar.2025.l886
Ucop Haroen, Syafwan Syafwan, Kiki Kurniawan, Agus Budiansyah, Nilawati Widjaja, Saitul Fakhri
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引用次数: 0

Abstract

Objective: This study aimed to identify the impact of variations in solvent polarity on the solubility of secondary metabolite compounds, which were correlated as antioxidant and antibacterial agents for Escherichia coli (ATCC 11725), Salmonella sp. (ATCC 22504), Staphylococcus aureus (ATCC 11526), and Bacillus subtilis (ATCC 11626).

Materials and methods: A total of 500 gm of Temulawak flour (Curcuma xanthorrhiza) was macerated gradually using the step gradient polarity (SGP) technique, where the solvents (methanol, ethyl acetate, and n-hexane) used had different levels of polarity. The extracting process of secondary metabolite components of Temulawak flour began with the use of non-polar solvents, semipolar solvents, and polar solvents, respectively. Each soaking process was completed for 5 × 24 h.

Results: From the Temulawak extract with different levels of solvent polarity, 76.048 gm were obtained for the methanol fraction, 106.242 gm for the ethyl acetate fraction, and 154.575 gm for the n-hexane fraction. The results of the antioxidant activity test showed that the ethyl acetate fraction had the highest inhibitory value for antioxidant activity of 50% (IC50) 22.59 mg/l. The results of this research's β-carotene content were 0.0865 gm. Ethyl acetate fraction was known to have good activity from the antibacterial activity test. The inhibition zone of E. coli and B. subtilis bacteria was 11.5 ± 0.71 with a minimum concentration of 3.13 mg/ml. The inhibition zone of S. aureus was 10.5 ± 0.17 with a minimum inhibitory concentration (MIC) of 6.25 mg/ml, while the inhibition zone for Salmonella sp. was 8.125 ± 0.35 with a MIC of 37.50 mg/ml.

Conclusion: All fractions have moderate antibacterial activity, yet the ability of the ethyl acetate fraction of Temulawak extract was higher than the methanol and n-hexane fractions.

不同溶剂极性姜黄提取物的酚类和类黄酮含量及生物活性。
目的:研究溶剂极性变化对大肠杆菌(ATCC 11725)、沙门氏菌(ATCC 22504)、金黄色葡萄球菌(ATCC 11526)和枯草芽孢杆菌(ATCC 11626)次生代谢产物溶解度的影响。材料和方法:选取500 gm Temulawak面粉(Curcuma xanthorrhiza),采用阶梯梯度极性(SGP)技术,在不同极性的溶剂(甲醇、乙酸乙酯和正己烷)中逐渐浸泡。采用非极性溶剂、半极性溶剂和极性溶剂分别提取特穆拉瓦克面粉次生代谢产物。结果:从不同溶剂极性水平的Temulawak提取物中,甲醇馏分为76.048 gm,乙酸乙酯馏分为106.242 gm,正己烷馏分为154.575 gm。抗氧化活性试验结果表明,乙酸乙酯部位抗氧化活性抑制值最高,为50% (IC50) 22.59 mg/l。结果表明,乙酸乙酯部位的β-胡萝卜素含量为0.0865 gm,具有较好的抗菌活性。对大肠杆菌和枯草芽孢杆菌的抑制带为11.5±0.71,最小浓度为3.13 mg/ml。对金黄色葡萄球菌的抑制带为10.5±0.17,最低抑制浓度(MIC)为6.25 mg/ml;对沙门氏菌的抑制带为8.125±0.35,最低抑制浓度(MIC)为37.50 mg/ml。结论:各部位均具有中等抑菌活性,其中乙酸乙酯部位抑菌活性高于甲醇和正己烷部位。
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来源期刊
CiteScore
2.70
自引率
7.10%
发文量
41
审稿时长
6 weeks
期刊介绍: Journal of Advanced Veterinary and Animal Research (JAVAR) - is an open access, international, peer-reviewed, quarterly, highly-indexed scientific journal publishing original research findings and reviews on all aspects of veterinary and animal sciences. Basic and applied researches on- - Anatomy & histology - Animal health economics - Animal nutrition - Animal reproduction - Animal science - Antimicrobial resistance (AMR) - Biochemistry - Biotechnology - Dairy science - Epidemiology - Food hygiene and technology - Genetics and breeding - Immunology - Microbiology - Parasitology - Pathology - Pharmacology & toxicology - Physiology - Poultry science - Preventive veterinary medicine - Public health - Surgery & obstetrics - Veterinary extension studies - Wildlife & aquatic medicine - Zoo animal medicine.
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