{"title":"螺旋藻对大鼠肝保护、抗炎和伤口愈合的作用。","authors":"Md Imran Hossain, Sabbya Sachi, Purba Islam, Popy Khatun, Kazi Rafiq, Md Zahorul Islam, Atsushi Miyamoto","doi":"10.5455/javar.2025.l897","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>This study aims to investigate the hepatoprotective, anti-inflammatory, and wound-healing potentials of spirulina utilizing a rat model.</p><p><strong>Materials and methods: </strong>Carbon tetrachloride (CCl<sub>4</sub>) was used to induce hepatotoxicity, while carrageenan was employed to induce hind paw inflammation. The wound healing capability was assessed by making a 6 mm round wound with a biopsy punch on the dorsal interscapular area of each rat. The animals were fed a diet mixed with spirulina at dosages of 250 and 500 mg.kg<sup>-1</sup>bwt. Paw thickness measurements were taken at 1, 3, and 6 h after carrageenan injection.</p><p><strong>Results: </strong>Intraperitoneal injection of CCl<sub>4</sub> leads to hepatotoxicity, as evidenced by significantly elevated serum levels of alanine aminotransferase and aspartate aminotransferase. Treatment with spirulina markedly reduced these biochemical markers. CCl<sub>4</sub>-induced hepatic cellular necrosis, central vein congestion, and steatosis were notably improved following spirulina administration. Additionally, spirulina treatment diminished paw edema and shortened wound closure time in a dose-dependent manner. Histopathological analysis of the inflamed paw revealed massive infiltration of inflammatory cells and thickening of the epidermis, both of which showed significant improvement with spirulina treatment. The anti-inflammatory and wound-healing effects of spirulina were comparable to those of indomethacin, an established anti-inflammatory drug.</p><p><strong>Conclusion: </strong>Our findings demonstrate that <i>Spirulina platensis</i> possesses beneficial properties in counteracting hepatotoxicity and inflammation. Additionally, it exhibited significant wound-healing effects in rat models. These results reinforce the potential health advantages of spirulina as an effective functional food.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 1","pages":"303-311"},"PeriodicalIF":1.5000,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186807/pdf/","citationCount":"0","resultStr":"{\"title\":\"Hepatoprotective, anti-inflammatory, and wound healing effects of spirulina in rats.\",\"authors\":\"Md Imran Hossain, Sabbya Sachi, Purba Islam, Popy Khatun, Kazi Rafiq, Md Zahorul Islam, Atsushi Miyamoto\",\"doi\":\"10.5455/javar.2025.l897\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>This study aims to investigate the hepatoprotective, anti-inflammatory, and wound-healing potentials of spirulina utilizing a rat model.</p><p><strong>Materials and methods: </strong>Carbon tetrachloride (CCl<sub>4</sub>) was used to induce hepatotoxicity, while carrageenan was employed to induce hind paw inflammation. The wound healing capability was assessed by making a 6 mm round wound with a biopsy punch on the dorsal interscapular area of each rat. The animals were fed a diet mixed with spirulina at dosages of 250 and 500 mg.kg<sup>-1</sup>bwt. Paw thickness measurements were taken at 1, 3, and 6 h after carrageenan injection.</p><p><strong>Results: </strong>Intraperitoneal injection of CCl<sub>4</sub> leads to hepatotoxicity, as evidenced by significantly elevated serum levels of alanine aminotransferase and aspartate aminotransferase. Treatment with spirulina markedly reduced these biochemical markers. CCl<sub>4</sub>-induced hepatic cellular necrosis, central vein congestion, and steatosis were notably improved following spirulina administration. Additionally, spirulina treatment diminished paw edema and shortened wound closure time in a dose-dependent manner. Histopathological analysis of the inflamed paw revealed massive infiltration of inflammatory cells and thickening of the epidermis, both of which showed significant improvement with spirulina treatment. The anti-inflammatory and wound-healing effects of spirulina were comparable to those of indomethacin, an established anti-inflammatory drug.</p><p><strong>Conclusion: </strong>Our findings demonstrate that <i>Spirulina platensis</i> possesses beneficial properties in counteracting hepatotoxicity and inflammation. Additionally, it exhibited significant wound-healing effects in rat models. These results reinforce the potential health advantages of spirulina as an effective functional food.</p>\",\"PeriodicalId\":14892,\"journal\":{\"name\":\"Journal of Advanced Veterinary and Animal Research\",\"volume\":\"12 1\",\"pages\":\"303-311\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2025-03-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186807/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Advanced Veterinary and Animal Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5455/javar.2025.l897\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/3/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Advanced Veterinary and Animal Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/javar.2025.l897","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/3/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Hepatoprotective, anti-inflammatory, and wound healing effects of spirulina in rats.
Objective: This study aims to investigate the hepatoprotective, anti-inflammatory, and wound-healing potentials of spirulina utilizing a rat model.
Materials and methods: Carbon tetrachloride (CCl4) was used to induce hepatotoxicity, while carrageenan was employed to induce hind paw inflammation. The wound healing capability was assessed by making a 6 mm round wound with a biopsy punch on the dorsal interscapular area of each rat. The animals were fed a diet mixed with spirulina at dosages of 250 and 500 mg.kg-1bwt. Paw thickness measurements were taken at 1, 3, and 6 h after carrageenan injection.
Results: Intraperitoneal injection of CCl4 leads to hepatotoxicity, as evidenced by significantly elevated serum levels of alanine aminotransferase and aspartate aminotransferase. Treatment with spirulina markedly reduced these biochemical markers. CCl4-induced hepatic cellular necrosis, central vein congestion, and steatosis were notably improved following spirulina administration. Additionally, spirulina treatment diminished paw edema and shortened wound closure time in a dose-dependent manner. Histopathological analysis of the inflamed paw revealed massive infiltration of inflammatory cells and thickening of the epidermis, both of which showed significant improvement with spirulina treatment. The anti-inflammatory and wound-healing effects of spirulina were comparable to those of indomethacin, an established anti-inflammatory drug.
Conclusion: Our findings demonstrate that Spirulina platensis possesses beneficial properties in counteracting hepatotoxicity and inflammation. Additionally, it exhibited significant wound-healing effects in rat models. These results reinforce the potential health advantages of spirulina as an effective functional food.