Journal of Advanced Veterinary and Animal Research最新文献

{"title":"Production and effects of nanomineral selenium (Nano-Se) feed additive on rumen fermentation, productivity, and reproductive performance of ruminants.","authors":"Pradita Iustitia Sitaresmi, Mohammad Firdaus Hudaya, Bayu Andri Atmoko, Wulandari Wulandari, Tri Ujilestari","doi":"10.5455/javar.2024.k830","DOIUrl":"10.5455/javar.2024.k830","url":null,"abstract":"<p><p>Nanotechnology (Nano) applications of feed additives can potentially improve feed-substrate efficiency to enhance livestock productivity. The utilization of Nano in feed in ruminants still tends to be under-explored and reviewed, particularly the application of Nano in trace minerals to enhance the reproductive performance and productivity of ruminants such as selenium. Trace minerals are essential for animal well-being and productivity, and the bioavailability of trace minerals is influenced by a complex matrix of interacting variables, including the chemical form of the minerals used and those found in the diet, the nature of the food ingested, the total composition of the diet, and the health and nutrition of the livestock. Nanominerals such as selenium (nano-Se) have shown impressive results when used as animal feed supplements in ruminants. Nano-Se can significantly boost wellness and immunity, gastrointestinal system function, microbiota homeostasis, metabolism, and reproductive performance in ruminants. This review aims to present the current knowledge on the technology of nano-Se in ruminants, ranging from the nanomanufacturing procedures of nano-Se, the impact of supplementation on the ruminant digestive system, productivity, and reproductive performance in ruminants in some dosages to find the optimized dosage to be provided.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 3","pages":"782-795"},"PeriodicalIF":1.5,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety and effectiveness of seahorse extract (<i>Hippocampus comes</i> L.) on the hematological profile and body weight of male rats induced by depo medroxyprogesterone acetate.","authors":"Trisnawati Mundijo, Franciscus Dhyanagiri Suyatna, Agung Eru Wibowo, Yusra Yusra, Yurnadi Hanafi Midoen","doi":"10.5455/javar.2024.k822","DOIUrl":"10.5455/javar.2024.k822","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to investigate the effectiveness and safety of seahorse <i>(Hippocampus comes</i> L.) extract on hematological profile and body weight on rats induced by depo medroxyprogesterone acetate (DMPA).</p><p><strong>Materials and methods: </strong>Thirty adult male Sprague-Dawley rats with 200-250 gm and 8 weeks old. All rats were intramuscularly administered 1.25 mg/kg BW DMPA Merck Depo Geston at 150 mg/3 ml. The animals were divided into five main groups (6 each), consisting of aquadest (G1), CMC 1% (G2), seahorse extract (SE) dose 150 mg/kg BW (G3), 225 mg/kg BW (G4), and 300 mg/kg BW (G5). All rats were weighed until the end of the treatment week.</p><p><strong>Results: </strong>The hematological profile and body weight of the group given SE tended to increase compared to the group not given extract; however, our hematological profile and body weight were in the normal range for rats.</p><p><strong>Conclusion: </strong>We find that SE enhances the effectiveness of the hematological profile, body weight, and safety of rats induced by DMPA.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 3","pages":"717-721"},"PeriodicalIF":1.5,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590595/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance and nutritional status of Holstein crossbred cows in a selected area of Bangladesh under the existing farming system.","authors":"Md Aliar Rahman, Rakhi Chowdhury, Khan Md Shaiful Islam","doi":"10.5455/javar.2024.k818","DOIUrl":"10.5455/javar.2024.k818","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to compare the body weight (BW), milk yield, nutritional status, and profitability of moderate genetic (MG) and high genetic (HG) merit of Holstein crossbred (HC) cows in a tropical region under the existing farming system.</p><p><strong>Materials and methods: </strong>Data was gathered from 204 nursing cows of MG (<i>n =</i> 99) and HG (<i>n =</i> 105) merit of HC cows throughout a year in the dairy zone Keraniganj, Bangladesh. HC cows of MG and HG merit contained 50.0%-67.7% and 75.0%-87.5% Holstein blood, respectively. Data on genetic merit, BW, lactation stage and number, daily milk yield, feed intake, feed, and milk price were documented. All variables were except genetic merit analyzed using one-way analysis of variance.</p><p><strong>Results: </strong>HC cows of MG and HG merit had 433 and 493 kg BW (<i>p</i> < 0.01), and daily produced 11.99 and 14.06 kg milk (<i>p</i> = 0.07) with having 0.99 and 1.15 feed efficiency (<i>p</i> = 0.06), respectively but dry matter intake did not vary (<i>p</i> > 0.05). HC cows of both genetic merit daily offered surplus metabolizable energy and digestible crude protein through roughage and concentrate than their requirement (<i>p</i> > 0.05). The milk production cost of both genetic merit HC cows was alike (<i>p</i> > 0.05), whereas almost two times more profit was obtained in HG merit HC compared to MG merit HC cows (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>HC cows of HG merit showed superior potentiality of milk yield, profit, and feed efficiency, whereas MG merit HC cows revealed inferior feed efficiency and milk yield.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 3","pages":"686-692"},"PeriodicalIF":1.5,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590593/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of zinc oxide nanoparticles utilizing extract from <i>Garcinia mangostana</i> leaves: Characterization and optimization of calcination temperature.","authors":"Ridho Kurniawan Rusli, Mustofa Hilmi, Maria Endo Mahata, Ahadyah Yuniza, Zurmiati Zurmiati, Sepri Reski, Rita Mutia, Cecep Hidayat","doi":"10.5455/javar.2024.k807","DOIUrl":"10.5455/javar.2024.k807","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to synthesize eco-friendly zinc oxide nanoparticles (ZnO NPs) by utilizing <i>Garcinia mangostana</i> leaf extract and assess the characteristics of ZnO NPs produced throughout different calcination temperatures (300°C, 400°C, 500°C, and 600°C).</p><p><strong>Materials and methods: </strong>An evaluation was conducted to analyze ZnO NPs using an aqueous extract of <i>G. mangostana</i> leaf bioreductor at different calcination temperatures. The analysis involved the use of a particle size analyzer (PSA), a scanning electron microscope (SEM), energy dispersive X-ray (EDX), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy.</p><p><strong>Results: </strong>The PSA and SEM indicated that the ZnO NPs had an average particle size ranging from 641.97 nm to 915.94 nm. Furthermore, the nanoparticles were found in both individual nanoforms and agglomerated forms. The EDX study indicated that the primary constituents of the ZnO NPs were zinc and oxygen. Additionally, the XRD examination demonstrated a distinct peak at 2θ = 36.25°, confirming the presence of a crystalline ZnO structure. The crystal size was determined to be between 40.98 nm and 46.92 nm. An FTIR spectroscopic study verified the existence of ZnO vibrations at distinct wavelengths as well as the absorption peak of the -OH functional group within the range of 3330.58 nm-3415.04 nm.</p><p><strong>Conclusion: </strong>The findings suggest that ZnO NPs produced utilizing the aqueous extract of <i>G. mangostana</i> leaves as a bioreductor can be synthesized at a temperature of 300°C, resulting in a lower particle size compared to those generated at 600°C.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 3","pages":"573-582"},"PeriodicalIF":1.5,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The antioxidant, anti-cholangiocarcinoma, and anti-<i>Opisthorchis viverrini</i> activities of ethanolic extract from <i>Antidesma thwaitesianum</i> fruit.","authors":"Ratchadawan Aukkanimart, Pranee Sriraj, Areeya Changthong, Tichanon Promsrisuk","doi":"10.5455/javar.2024.k813","DOIUrl":"10.5455/javar.2024.k813","url":null,"abstract":"<p><strong>Objectives: </strong>The current study was performed to determine the antioxidant, anti-inflammatory, and anti-cholangiocarcinoma (CCA) properties of <i>Antidesma thwaitesianum,</i> also known as \"MAO,\" whole plant extract on Opisthorchiasis in animal models and CCA cell lines.</p><p><strong>Materials and methods: </strong>Ethanol was used to extract compounds from the whole ripe fruit. The phytochemical investigation of MAO extract was done to evaluate antioxidant activity, and high-performance liquid chromatography was used to identify the active compounds. The efficacy of MAO extract against OV was evaluated <i>in vivo</i>. The anti- CCA activity was evaluated using superoxide dismutase (SRB), cell cycle arrest, apoptosis, and western blot analyses.</p><p><strong>Results: </strong>MAO extract possessed flavonoid and phenolic contents, antioxidant activity, and an expressed cyanidin-3-O-glycosides content of 0.08 μg/mg extract. MAO extract demonstrated hepatoprotective effects through raised alanine transaminase and alkaline phosphatase levels, as well as an influence on oxidative stress via decreased MDA and increased glutathione, superoxide dismutase, and catalase levels. MAO extract significantly inhibited the migration of CCA cells in a concentration- and time-dependent manner, as well as triggered cell cycle arrest on G1 and activated apoptosis pathways via upregulation of C3, downregulation of cyclin-dependent kinase inhibitor p21, cyclin D, and cyclin-dependent kinases 2 expression. MAO extract inhibited inflammation, which in turn decreased fibrosis in hamsters. It also increased hepatoprotective activity.</p><p><strong>Conclusion: </strong>Our findings demonstrate the potential benefits of MAO extract in both <i>in vitro</i> and animal studies of hamster opisthorchiasis. However, more research should be done to ascertain the mechanisms of activity of MAO extracts and elute bioactive components in order to confirm their safety and examine their clinical applications.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 3","pages":"637-647"},"PeriodicalIF":1.5,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590606/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EphB4 and ephrin-B1 expression in the intra-testicular-resident macrophages in mice.","authors":"Md Royhan Gofur, Kazushige Ogawa","doi":"10.5455/javar.2024.k826","DOIUrl":"10.5455/javar.2024.k826","url":null,"abstract":"<p><strong>Objective: </strong>The objective was to find out the expression of EphB4 receptor and ephrin-B1 ligand by the macrophages that live inside the mouse testicles.</p><p><strong>Materials and methods: </strong>Messenger ribonucleic acid (mRNA) expression of EphB4 and ephrin-B1 was identified via RT-PCR amplification, and protein expression was examined by immunostaining.</p><p><strong>Results: </strong>Analysis using RT-PCR revealed that mRNA of EphB4 and ephrin-B1 were noticed in the examined testis of all postnatal ages. Furthermore, immunostaining revealed that F4/80-positive intra-testicular-resident macrophages were located in the intertubular spaces within the testis and more densely around the intra-testicular excurrent duct system, and increased in number gradually during the postnatal period of development until 5 weeks of age, when the mice attain their maturity (puberty), and maintained thereafter. Both EphB4 and ephrin-B1 immunoreactivity were noticed in F4/80-positive intra-testicular-resident macrophages within the testis of all studied postnatal ages. Ephrin-B1 and EphB4 immunoreactivity were weak during early postnatal development until the age of 2 weeks, and then ephrin-B1 immunoreactivity became very strong and EphB4 immunoreactivity became strong at the age of 3 weeks, and they continued to do so until the age of 8 weeks. Furthermore, EphB4 receptor was tyrosine-phosphorylated in testis.</p><p><strong>Conclusion: </strong>The expression of EphB4 and ephrin-B1 in mice intra-testicular-resident macrophages is being examined for the first time in this work. The localization of EphB4 and ephrin-B1, and EphB4 tyrosine-phosphorylation suggest that EphB4/ephrin-B1 signaling might occur in the intra-testicular-resident macrophages, and may participate in maintaining male fertility.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 3","pages":"746-753"},"PeriodicalIF":1.5,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590604/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infectivity and molecular epidemiology of Peste des Petits ruminants virus in slaughtered goats at the local meat market of Mymensingh division, Bangladesh.","authors":"Sajeda Sultana, Munmun Pervin, Nazneen Sultana, Muhammad Tofazzal Hossain, RMd Afiqul Islam, Mohammad Abu Hadi Noor Ali Khan","doi":"10.5455/javar.2024.k815","DOIUrl":"10.5455/javar.2024.k815","url":null,"abstract":"<p><strong>Objective: </strong>The purpose of this study was to characterize the circulating Peste des Petits Ruminants virus (PPRV) from slaughtered goats and conduct a phylogenetic analysis of the N gene of PPRV.</p><p><strong>Materials and methods: </strong>A total of 196 slaughtered goats were investigated at the marketplaces of Mymensingh division from January 2019 to March 2021. Lungs, spleen, and mesenteric lymph nodes were collected for histology and molecular study. In-house developed Reverse-Transcriptase-Polymerase Chain Reaction (RT-PCR) protocols were carried out using designed primer sets (PPRV NF-gctctgtgattgcggctgagc and PPRV NR-cctggtcctccagaatcttggcc). The CLC sequence viewer was used for phylogenetic analysis.</p><p><strong>Results: </strong>Grossly pneumonic lungs, shrinkage spleen, and enlarged mesenteric lymph nodes with hemorrhages were recorded. Both intracytoplasmic and intranuclear inclusion bodies were seen in lymphocytes of the mesenteric lymph node, spleen, and lungs. PPRV was detected in 37 goats (18.9%) by RT-PCR test. The 402-bp amplicon was generated in PPRV-positive cases. The phylogenetic analysis showed that the studied PPRV isolates of the Mymensingh division belonged to lineage IV.</p><p><strong>Conclusion: </strong>The prevalence of PPR was 18.9% in slaughtered goats at marketplaces in the Mymensingh division. Slaughterhouses may be a source of PPRV, and it can be horizontally transmitted from the meat market to the farm. Restricting sick animal movement within the country, mass PPR vaccination campaigns, increased awareness, and improved biosecurity in the meat market may lessen the incidence of PPR in goats.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 3","pages":"655-663"},"PeriodicalIF":1.5,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590596/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rumen-protected lysine and methionine failed to improve the performance of late-nursing Awassi ewes regardless of dietary protein contents.","authors":"Mofleh S Awawdeh","doi":"10.5455/javar.2024.k821","DOIUrl":"10.5455/javar.2024.k821","url":null,"abstract":"<p><strong>Objective: </strong>The current study investigated the effects of supplying ruminally protected amino acids (AA) (lysine, L; and methionine, M) and dietary protein levels on the performance of late-nursing ewes.</p><p><strong>Materials and methods: </strong>Thirty-one Awassi ewes nursing single lambs were individually housed and assigned randomly to one of four treatment groups (2 × 2 factorial design). Ewes in treatment groups were (with supplemental RPL and RPM) or were not (without supplemental RPL and RPM) supplemented with lysine (8.5 gm/day) and methionine (4 gm/day) and were fed diets containing either 13.2 (moderate protein) or 11.1% (low protein) protein.</p><p><strong>Results: </strong>No interactions between supplemental AA and dietary protein levels were observed. Supplying ewes with L and M did not affect (<i>p</i> ≥ 0.06) their nutrient intake or their final body weights (BWs). Additionally, milk composition, yield, and efficiency were not affected by supplemental L and M. Decreasing dietary protein levels did not affect (<i>p</i> = 0.13) the final BWs, milk yield, composition, and efficiency but decreased (<i>p</i> < 0.01) nutrient intake of ewes.</p><p><strong>Conclusion: </strong>Under our study conditions, reducing the protein contents of the diets from 13.2% to 11.1% had no negative impact on late-nursing ewes. Regardless of dietary protein level, the beneficial effect of supplying L and M was not evident.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 3","pages":"711-716"},"PeriodicalIF":1.5,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of GHG emissions in dairy production systems based on existing feed resources through the <i>GLEAM</i> model under different climatic zones of Bangladesh and their mitigation options.","authors":"Muhammad Khairul Bashar, Nathu Ram Sarker, Nasrin Sultana, Sheikh Mohammad Jahangir Hossain","doi":"10.5455/javar.2024.k816","DOIUrl":"10.5455/javar.2024.k816","url":null,"abstract":"<p><strong>Objective: </strong>The current study evaluated the greenhouse gas (GHG) emissions of dairy cattle through the Global Livestock Environmental Assessment Model <i>(GLEAM)</i> model and illustrated potential mitigation strategies by modifying nutrition interventions.</p><p><strong>Materials and methods: </strong>A semi-structural questionnaire was developed to calculate dairy animal GHG emissions. This study comprised 40 farmers from four districts: river basin (Pabna), drought-prone (Chapainobabganj), floodplain (Nilphamari), and saline-prone (Sathkhira) areas. Ten lactating cows (two cows from each farmer) were also selected to collect information on feeding practices, feed resources, feed intake (roughages and concentrate), water intake, and productive and reproductive parameters for 7 days at each site during two seasons: dry (November-February) and wet (June-October).</p><p><strong>Results: </strong>The GHG emissions from the river basin area were significantly (<i>p</i> < 0.05) higher due to low-quality roughages (75%), whereas CH₄/kg of milk production was the lowest (77.0 gm). In contrast, the area that frequently experiences drought showed a different pattern. For instance, the generation of CH₄ from enteric fermentation was 1187.4 tons/year, while the production of CH₄ and N₂O from manure management was 323.1 tons/year and 4.86 tons/year, respectively. In comparison to other climatic areas, these values were the lowest because the supply of green grass was twice as abundant as in the other climatic areas (40%). The quantity of CH₄/kg of milk produced in an area susceptible to drought did not vary.</p><p><strong>Conclusion: </strong>Implementing feeding systems in drought-prone areas is a successful approach to reducing GHG emissions in the dairy industry in Bangladesh. Consequently, implementing feed-balancing techniques can enhance productivity and foster environmentally sustainable animal production.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 3","pages":"664-674"},"PeriodicalIF":1.5,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of <i>Neospora caninum</i> from aborted fetuses of cattle, sheep, and goats in Bangladesh.","authors":"Md Shahiduzzaman, Pijush Biswas, Ajran Kabir, Abu Rakib M Beni Amin, Sakhyajit Saha Parijat, Nurnabi Ahmed, Md Zawad Hossain, Majed H Wakid","doi":"10.5455/javar.2024.k811","DOIUrl":"10.5455/javar.2024.k811","url":null,"abstract":"<p><strong>Objectives: </strong>The study aimed to detect <i>Neospora caninum</i> by nested PCR (nPCR) in aborted fetuses of cattle, sheep, and goats in Bangladesh.</p><p><strong>Materials and methods: </strong>The head portion of each aborted fetus (111) was dissected at each sampling site and transferred to the laboratory in an ice box. Data on risk factors associated with <i>N. caninum</i> infection were simultaneously collected. Deoxyribonucleic acid was extracted from brain tissue to perform nPCR targeting the internal transcribed spacer 1 (ITS1) ribosomal DNA (rDNA) gene of <i>N. caninum</i> and sequencing was performed from the representative positive samples<i>.</i></p><p><strong>Results: </strong>By nPCR, <i>N. caninum</i> was found in 16.0% of aborted fetuses of cattle, followed by sheep (14.81%) and goats (11.78%). The highest prevalence was found in aborted fetuses of animals during the second trimester (27.78%) of pregnancy aged 2 to 4 years (18.75%). Obtained sequences showed they were completely matched with <i>N. caninum</i> ITS1 rDNA gene deposited in GenBank. Univariate analysis demonstrated that pregnancy stages (trimesters), abortion history of the animals, and access to dogs in animal farms were significantly (<i>p</i> ≤ 0.05) correlated with <i>N. caninum</i> infection.</p><p><strong>Conclusion: </strong>This study represents the first investigation into the molecular detection, phylogenetic characterization, and analysis of risk factors associated with <i>N. caninum</i> in livestock in Bangladesh. According to the research findings, <i>N. caninum</i> infection may have a role in abortion cases and the ensuing financial losses in the nation's livestock industry.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 3","pages":"618-626"},"PeriodicalIF":1.5,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590592/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}