Journal of Advanced Veterinary and Animal Research最新文献

{"title":"Genetic variations of <i>Toll-like receptor 4</i> gene in exon 2 of South African Dorper sheep.","authors":"Lebelo Selala, Teedzai Chitura, Vusi Mbazima, Louis Tyasi","doi":"10.5455/javar.2024.k777","DOIUrl":"10.5455/javar.2024.k777","url":null,"abstract":"<p><strong>Objective: </strong>The study was conducted to identify the sequence variation of <i>Toll-like receptor 4</i> (<i>TLR4)</i> in exon 2 of South African Dorper sheep.</p><p><strong>Materials and methods: </strong>Blood samples were collected from fifty (<i>n =</i> 50) South African Dorper sheep aged between 3 and 4 years. The Deoxyribonucleic acid (DNA) was extracted, amplified, and sequenced for the <i>TLR4</i> gene. DNA sequencing was used to identify the sequence variations of the <i>TLR4</i> gene in South African Dorper sheep.</p><p><strong>Results: </strong>The results showed that one synonymous single nucleotide polymorphism (SNP) of the <i>TLR4</i> gene in exon 2 position T2249C was identified. Two genotypes (TT and TC) were discovered from the identified SNP. The dominant genotype was TT (0.60) over TC (0.40), with the dominant allele T (0.80) over C (0.20). The results also indicated that the used population was in the Hady-Weinberg Equilibrium. Polymorphism genetic analysis findings suggest that the identified sequence variation of TLR4 in exon 2 of South African Dorper sheep was moderate polymorphism.</p><p><strong>Conclusion: </strong><i>TLR4</i> gene at exon 2 of South African Dorper sheep had the SNP (T>C) at position 2249 bp with two genotypes (TT and TC).</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11296168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An investigation of antioxidative and anti-inflammatory effects of <i>Taraxacum coreanum</i> (white dandelion) in lactating Holstein dairy cows.","authors":"Sung Jae Kim, Young Jun Jo, Sang-Hee Jeong, Yo-Han Kim, Jeong Hee Han","doi":"10.5455/javar.2024.k781","DOIUrl":"10.5455/javar.2024.k781","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this investigation was to examine the impact of <i>Taraxacum coreanum</i> (known as dandelion) (TC) and TC mixtures with milk thistle (MT) or <i>Aspergillus oryzae</i> (AO) as feed additives on the immune response, milk quality, and milk production in Holstein cows over 6 weeks of administration.</p><p><strong>Materials and methods: </strong>Thirty-two healthy Holstein dairy cows were provided 30 kg of total mixed ration (TMR) with no TC, 90 gm TC, 54 gm TC + 36 gm MT, or 54 gm TC + 36 gm AO 40% groups. The feed additives were supplied daily in two equal portions (per 45 gm) by topdressing the TMR for 6 weeks. Milk and blood samples were collected weekly.</p><p><strong>Results: </strong>In the TC-treated cows (TC, TC + MT, and TC + AO groups), significantly lower peripheral blood white blood cell (WBC) counts at 6 weeks and milk somatic cell counts (SCCs) at 4-6 weeks of administration were observed. Concentrations of serum superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) were notably elevated in cows treated with TC for 4-6 weeks, while levels of proinflammatory cytokines concentrations of tumor necrosis factor-alpha (TNF-α) and chemokine (IL-8) were significantly reduced in TC-treated cows after 3-6 weeks of administration.</p><p><strong>Conclusion: </strong>These results suggested that TC or a TC mixture with other medicinal herbs supplementations enhanced the serum antioxidative activities and, consequently, might suppress the adverse immune response due to lower serum TNF-α and IL-8 release supported by lower WBC and SCC counts.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11296163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of rumen contents' characteristics in Nguni and Bonsmara cows raised under two different grazing systems.","authors":"Denis Kayima, Mhlangabezi Slayi, Ishmael Festus Jaja, Cletos Mapiye, Kennedy Dzama","doi":"10.5455/javar.2024.k783","DOIUrl":"10.5455/javar.2024.k783","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate rumen fermentation parameters influenced by both grazing system and breed.</p><p><strong>Materials and methods: </strong>A 2 × 2 factorial design was employed, involving 40 cows with matched age, parity, and physiological status. The cows were evenly divided between Bonsmara and Nguni breeds, as well as communal and commercial grazing systems. Rumen fluid samples were collected and analyzed for parameters including ammonia-nitrogen (NH3-N), pH, temperature, and volatile fatty acids (VFAs).</p><p><strong>Results: </strong>Nguni cows exhibited significantly higher ruminal NH3-N levels (<i>p <</i> 0.05) compared to Bonsmara, ranging from 69.05 to 96.78 mg/l. Commercial grazing demonstrated significantly higher NH3-N concentrations (<i>p <</i> 0.05) than communal grazing. Ruminal pH, temperature, total VFAs, and specific VFAs (Iso-butyrate, valeric, and iso-valeric) did not show significant differences (<i>p ></i> 0.05). However, total VFAs were slightly lower in communal grazing (78.87 mmol/l) than in commercial grazing (89.80 mmol/l). Acetate, propionate, butyrate, and the acetate to propionate ratio did not display significant differences (<i>p ></i> 0.05) between breeds but varied between grazing systems. Communal systems had higher acetate and acetate to propionate ratio (<i>p <</i> 0.05), while commercial systems showed higher propionate and butyrate levels (<i>p <</i> 0.05).</p><p><strong>Conclusion: </strong>Grazing conditions significantly influenced rumen fermentation parameters, irrespective of breed. Further research is necessary to explore the relationship between forage conditions, diversity, and rumen fermentation within different grazing systems.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11296167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the effectiveness of some essential oils against zoonotic methicillin-resistant <i>Staphylococcus aureus</i> isolated from dairy products and humans.","authors":"Marwa B Salman, Asmaa Ibrahim Abdelaziz Zin Eldin, Nourhan Eissa, Ahmed Maher, Abd-Elghany Aish, Sherein I Abd El-Moez","doi":"10.5455/javar.2024.k778","DOIUrl":"10.5455/javar.2024.k778","url":null,"abstract":"<p><strong>Objective: </strong><i>Staphylococcus aureus</i> (<i>S. aureus</i>) is a zooanthroponotic, nosocomial, and community-associated pathogen that threatens livestock management and even public health. The goal of this investigation was to clarify the role of <i>S. aureus</i> in zoonotic illnesses. Besides that, a novel trial was conducted in the current Egyptian study using oil extracts such as cactus oil, tea oil, geranium oil, and thyme oil to demonstrate the susceptibility of methicillin-resistant <i>S. aureus</i> (MRSA) isolates to these organic oils in response to the alarming global concern regarding the decreased susceptibility of <i>S. aureus</i> to known antibiotics, which exacerbates control and treatment protocols.</p><p><strong>Materials and methods: </strong>A total of 110 samples (45 raw cattle milk samples, 35 Karish cheese samples, and 30 human sputum samples) were collected. The bacterium was identified via traditional culturing methods, Gram staining, and the application of several biochemical tests. After that, various kinds of known commercial antibiotics were used to detect the antimicrobial susceptibility (AMS) of the obtained isolates. Furthermore, conventional polymerase chain reaction (PCR) testing was performed to identify <i>S. aureus</i> (<i>nuc</i> gene) and MRSA (<i>mec</i>A gene), with further application of multiplex PCR for screening of all the obtained isolates for vancomycin resistance via targeting <i>van</i>A, <i>van</i>B, and <i>van</i>C genes. Finally, the agar gel diffusion method was performed to assess the antibacterial activity of four plant extracts (cactus oil, tea oil, geranium oil, and thyme oil) against the obtained MRSA.</p><p><strong>Results: </strong>The culturing method revealed <i>S. aureus</i> positivity in raw cattle milk (13.33%), in Karish cheese (28.57%), and in human samples (20%). The obtained isolates showed mainly resistance to amoxicillin-clavulanic and ampicillin antibiotics, while the dairy samples showed further resistance against ceptaxime and an intermediate reaction against erythromycin. On the molecular side, PCR positivity was present in human samples (10%), raw cow milk (13.33%), and Karish cheese (14.29%). Nine of the fourteen PCR isolates were methicillin-resistant <i>S. aureus</i> (MRSA) isolates. Comparing the four oil extracts against the acquired MRSA isolates, cactus oil extract proved to be the most effective.</p><p><strong>Conclusion: </strong>The study's results are highly promising as they support the notion that certain essential oils possess strong antimicrobial properties against zoonotic <i>S. aureus</i>, thereby reducing the excessive use of antibiotics in veterinary and medical settings.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11296189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting dog tracheal diameter and length: A tool for improved intubation.","authors":"Maneenooch Khiao-In, Suppada Kananub, Tepyuda Sritrakul, Pattarawadee Thamsatit, Sirin Theerawatanasirikul, Naparee Srisowanna","doi":"10.5455/javar.2024.k780","DOIUrl":"10.5455/javar.2024.k780","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to develop formulas for estimating tracheal diameter and length in dogs using easily measurable anatomical parameters.</p><p><strong>Materials and methods: </strong>The samples consisted of 20 dogs of various breeds, comprising 10 males and 10 females, sourced from cadavers. The measured parameters included occipital tuberosity to tail base (OT), eye angle to ear tragus, nose to ear tragus, inner vertical diameters (IVD), and tracheal length (TL). The study conducted correlation and linear regression analyses, and subsequently, the formulated models underwent validation using 16 live dogs. The results were compared to radiographic measurements.</p><p><strong>Results: </strong>Linear regression recommended formulas based on OT, resulting in IVD (mm) = 0.203 × OT - 3.724 (<i>r² =</i> 0.608, <i>p</i> < 0.001) and TL (cm) = 0.346 × OT-3.773 (<i>r² =</i> 0.837, <i>p</i> < 0.001). The predicted tracheal diameter and length from formulas were slightly smaller than radiographic measurements (IVD = 2.76 ± 1.85 mm, <i>p</i> < 0.0001 and TL = 2.07 ± 1.81 cm, <i>p</i> < 0.0001).</p><p><strong>Conclusion: </strong>These formulas offer a practical way to estimate tracheal dimensions in live dogs, facilitating the selection of suitable endotracheal tube sizes and insertion depth. Further studies with larger sample sizes and consistent measurement methods can enhance the accuracy of these findings.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11296161/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dietary <i>Moringa oleifera</i> on production performance and gut health in broilers.","authors":"Shumaila Gul, Fida Hussain, Raheela Taj, Asad Ullah","doi":"10.5455/javar.2024.k782","DOIUrl":"10.5455/javar.2024.k782","url":null,"abstract":"<p><strong>Objective: </strong>In the present research work, we examined the dietary <i>Moringa oleifera</i> effect on gut health and growth traits in chickens.</p><p><strong>Materials and methods: </strong>There were 280 chicks (day old) that were weighted and allotted uniformly in seven groupings, each containing eight replicates (<i>n =</i> 5). Birds were supplemented with <i>M. oleifera</i> leaf extract (MLE) and seed extract (MSE) for 35 days. Group I was the control (fed merely basal diets), while Group II received 0.8% MLE, Group III was given 0.8% MSE, Group IV was given 1.2% MLE, Group V was given 1.2% MSE, Group VI was given 0.8% MLE + 0.8% MSE, and Group VII was given 1.2% MLE + 1.2% MSE. At the end of the fifth week, two chickens were selected from each replica, and samples (small intestine and ileal ingesta) were collected.</p><p><strong>Results: </strong>The chicken diet with MLE and MSE supplements saw significant improvement (<i>p</i> < 0.05) in both feed conversion ratio (FCR) and body weight gain (BWG). In the small intestine (duodenal, jejunal, and ileal), dietary MLE and MSE supplements significantly increased (<i>p</i> < 0.05) the surface area of the villus and the ratio of their height/crypt depth in comparison to the control group. The MLE and MSE supplements significantly increased (<i>p</i> < 0.05) the total goblet cell counts in the small intestine. The Lactobacillus <i>spp.</i> count was significantly improved (<i>p</i> < 0.05) and reduced (<i>p</i> < 0.05) in <i>Escherichia coli</i> counts when the bird diet was supplemented with MLE (0.8%) and MSE (0.8%).</p><p><strong>Conclusion: </strong>Results indicated that <i>M. oleifera</i> leaf and seed extract diet improved the growth trait and gut health in chickens.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11296185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DMP-1 expression in alveolar bone socket following <i>Anredera cordifolia</i> (Ten.) Steenis treatment: A histological study.","authors":"Christian Khoswanto, Ira Kusuma Dewi","doi":"10.5455/javar.2024.k775","DOIUrl":"10.5455/javar.2024.k775","url":null,"abstract":"<p><strong>Objective: </strong>The study aimed to ascertain how <i>Anredera cordifolia</i> (Ten.) Steenis Gel affects the expression of protein dentin matrix protein-1 (DMP-1) in alveolar Wistar rats after tooth extraction.</p><p><strong>Materials and methods: </strong>Rats were given <i>A. cordifolia</i> (Ten.) Steenis gel was in the socket after tooth extraction, and then the wound was sutured. The rats were sacrificed for 8 and 15 days following tooth extraction. The results on the 8^th and 15^th days demonstrate that the expression of DMP-1 in the treatment group is significantly higher than in the control group.</p><p><strong>Results: </strong>Expression of DMP-1 in the socket after tooth extraction on days 8 and 15 with a 400x magnification light microscope in both of the <i>A. cordifolia</i> (Ten.) Steenis gel treatment groups showed significant differences compared to the control group.</p><p><strong>Conclusion: </strong>The use of <i>A. cordifolia</i> (Ten.) Steenis gel can stimulate DMP-1 expression in alveolar bone after tooth extraction.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11296160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of Andaliman (<i>Zanthoxylum acanthopodium</i> DC.) fruit extracted with ethanol on TNF-α and TRPA-1 levels in type II diabetes-induced mice.","authors":"Boyke Marthin Simbolon, O K Yulizal, Albert Manggading Hutapea, Erwin Handoko","doi":"10.5455/javar.2024.k774","DOIUrl":"10.5455/javar.2024.k774","url":null,"abstract":"<p><strong>Objective: </strong>The present study investigated the effects of Andaliman fruit extract on tumor necrosis factor-alpha (TNF-α) and transient receptor potential ankyrin-1 (TRPA-1) levels in type 2 diabetes mellitus (T2DM) mouse models induced with streptozocin (STZ) and a high-fat diet (HFD).</p><p><strong>Materials and methods: </strong>In this research, mice were allocated into six distinct groups: normal, negative control (HFD and STZ), positive control (metformin, HFD, and STZ), and three treatment groups (HFD, STZ, and Andaliman extract at varying dosages of 100, 300, and 500 mg/kg, respectively). Body weight and blood glucose levels (BGLs) were recorded at weeks 1 (baseline), 8, 12, and 16. The levels of TNF-α and TRPA-1 were measured during the 16th week.</p><p><strong>Results: </strong>Phytochemical screening of the Andaliman extract revealed the presence of flavonoids, alkaloids, tannins, saponins, and glycosides. The one-way ANOVA revealed significantly elevated BGL at week 16 in the negative control group in comparison to the other groups (<i>p</i> < 0.05). The Kruskal-Wallis test followed by Bonferroni-corrected pairwise comparisons showed that the negative control had significantly higher TNF-α levels than the Andaliman-groups (<i>z</i> = 22.11, <i>p <</i> 0<i>.</i>01). TRPA-1 was significantly higher in the negative control group compared to the treatment groups (<i>p</i> < 0.05). Furthermore, Spearman's rho analysis revealed a statistically significant positive association between BGL and both TNF-α and TRPA-1, as well as between TNF-α and TRPA.</p><p><strong>Conclusion: </strong>Andaliman extract potentially serves as a therapy for diabetic neuropathy in T2DM by lowering BGL and inhibiting the expression of TNF-α and TRPA-1.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11296190/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The utilization of pineapple hump extracts in complete feeds on the quality of free-board rabbit meat.","authors":"Galih Ari Wirawan Siregar, Rini Hardiyanti, Uswatun Hasanah, Kennie Cendekia Desnamrina, Ferdy Saputra, Bram Brahmantiyo","doi":"10.5455/javar.2024.k776","DOIUrl":"10.5455/javar.2024.k776","url":null,"abstract":"<p><strong>Objective: </strong>The study aimed to identify the effect of pineapple hump extracts in different doses on increasing the chemical and physical quality of rabbit meat.</p><p><strong>Materials and methods: </strong>The research stages were carried out with maintenance for two months using a completely randomized design consisting of four treatments and five replicates. P0 = complete ration without pineapple hump extract; P1 = complete ration with the addition of 0.2% pineapple hump extract; P2 = complete ration with the addition of 0.4% pineapple hump extract; and P3 = complete ration with the addition of 0.6% pineapple hump extract. The variables observed were the chemical quality of meat (pH, moisture, carbohydrate, fat, and protein content in rabbit meat), and the physical quality (water holding capacity (WHC), cooking loss).</p><p><strong>Results: </strong>The data were analyzed using SAS, with significantly different results subjected to Duncan's further testing. The addition of 0.4% pineapple hump extract in rabbit complete rations had a very significant effect on the WHC of 71.62%. The addition of 0.6% pineapple hump extract also had a significant effect on the protein content of meat, increasing it by 19.17%.</p><p><strong>Conclusion: </strong>The effects of pineapple hump extract up to 0.6% in a complete diet of weaned rabbits have a positive effect on the physical and chemical quality of rabbit meat, especially on the protein and water-holding capacity of rabbit meat.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11296177/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Ampiang-Dadih-</i>a combination of Indonesian traditional fermented buffalo milk and black glutinous rice-prevents hypercholesterolemia and liver cell degeneration <i>in vivo</i>: A pilot study.","authors":"Sri Rahmatul Laila, Eddy Sukmawinata, Falsa Martiana Kencana Putri, Ilham Akbar, Luthfiyyah Fitri Zahra, Srihadi Agungpriyono, Umi Cahyaningsih, Tutik Wresdiyati","doi":"10.5455/javar.2024.k773","DOIUrl":"10.5455/javar.2024.k773","url":null,"abstract":"<p><strong>Objective: </strong>We aimed to evaluate the potential of <i>Ampiang-Dadih</i> (AD), a combination of Indonesian traditional fermented buffalo milk (<i>Dadih</i>) and black glutinous rice flakes (<i>Ampiang</i>) as an anti-hypercholesterolemic agent and to prevent liver-cell degeneration using a rat model.</p><p><strong>Materials and methods: </strong>A mixture of black glutinous rice powder (0.3 gm/gm feed) and fermented buffalo milk (3.74/200 gm BW) was prepared to obtain AD. Fifteen adult male Sprague Dawley rats were divided into three groups of five animals each: (A) negative control group (distilled water; 5 weeks), (B) hypercholesterolemia group (1% cholesterol per feed; 5 weeks), and (C) preventive AD group (1% cholesterol and AD; 5 weeks). The blood lipid profiles were measured at weeks 2, 4, and 5. The liver enzyme activity, cholesterol level, and histology were observed at the end of week 5.</p><p><strong>Results: </strong>AD administration simultaneously with cholesterol in Group C significantly prevented an increase in total plasma cholesterol and low-density lipoprotein levels compared to Group B. Alanine transaminase and aspartate transaminase were maintained at normal levels in Group C. Furthermore, the levels of liver cholesterol and liver cell degeneration in Group C were also maintained because of AD administration compared to that in Group B.</p><p><strong>Conclusion: </strong>This study demonstrated that AD has the potential to be developed as a functional food for hypercholesterolemia prevention.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11296179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}