Journal of Advanced Veterinary and Animal Research最新文献

{"title":"Developing a simple universal hypo-osmotic swelling test (HOST) for assessing sperm membrane integrity in pigs, rabbits, and goats.","authors":"Khuong Thi Thanh Tran, Tan Nhat Nguyen, Duy Lam Khanh Nguyen","doi":"10.5455/javar.2025.l913","DOIUrl":"10.5455/javar.2025.l913","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to evaluate the integrity of the plasma membrane for human and other domestic animal spermatozoa by the hypo-osmotic swelling test (HOST).</p><p><strong>Materials and methods: </strong>This study was conducted on spermatozoa of three species, including pigs, rabbits, and goats. Three experiments were conducted on three animal species (pigs, rabbits, and goats) to investigate the factors affecting sperm membranes: osmotic concentration (0, 50, 100, and 150 mOsmol) and treatment time (0, 15, 30, 45, 60, and 90 min) at 37°C.</p><p><strong>Results: </strong>The results showed that treatment time and osmotic concentration influenced the integrity of the sperm membrane in the hypo-osmotic solution. In general, the experiments showed a high rate of sperm reacting to HOST at 50 and 100 mOsmol for 30-45 min. According to this study, the hypo-osmotic solution resulted in a high rate of sperm with swollen tails and a high rate of viable sperm, indicating a correlation between these factors. Therefore, it is necessary to combine both conditions to assess sperm quality. Specifically, the positive rates for the HOST reaction in pig, rabbit, and goat sperm are 46.74%, 58.28%, and 61.95%, respectively.</p><p><strong>Conclusion: </strong>To sum up, the hypo-osmotic solution with a concentration of 100 mOsmol and an incubation time of 45 min is considered the optimal and most feasible condition that can be used for all three species: pigs, rabbits, and goats.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 2","pages":"477-486"},"PeriodicalIF":1.5,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12506764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of interferon on broilers' fecal microbiome composition.","authors":"Inna Burakova, Yuliya Smirnova, Mariya Gryaznova, Polina Morozova, Vyacheslav Kotarev, Ludmila Lyadova, Nadezhda Ivanova, Larisa Denisenko, Mikhail Syromyatnikov","doi":"10.5455/javar.2025.l914","DOIUrl":"10.5455/javar.2025.l914","url":null,"abstract":"<p><strong>Objective: </strong>The purpose of our study was to investigate the effect of chicken interferon on the intestinal microbiota of broiler chickens.</p><p><strong>Materials and methods: </strong>The study used next-generation sequencing on the Ion Torrent pragmatic general multicast platform to target the V3 <i>16S ribosomal ribonucleic acid</i> hypervariable region gene, allowing us to analyze in detail changes in the composition of the broiler chicken microbiome.</p><p><strong>Results: </strong>Forty-one bacterial genera were identified in the studied groups of broilers. The highest abundance in both groups was observed for <i>Lactobacillus</i>, which was 31.08% ± 6.52 in the control group and 36.08% ± 7.25 in the interferon group. There was no clustering between the microbiome communities of the groups studied. We found a decrease or complete absence of <i>Escherichia-Shigella, Eubacterium fissicatena group, Lachnospiraceae CHKCI001,</i> and <i>Pediococcus</i> in the interferon-treated broiler group compared to the control group and an increase in the number of genera <i>Ruminococcaceae CAG-352</i> and <i>Turicibacter</i> in the interferon group.</p><p><strong>Conclusion: </strong>A decrease in <i>E.-Shigella</i> may indicate normalization of the intestinal microbiota of broilers, and it can also be concluded that the introduction of interferon helps to suppress opportunistic bacteria. In the interferon group, a sharp increase in the number of <i>Turicibacter</i> was observed. Representatives of this genus are among the most common members in the intestines of broilers.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 2","pages":"487-496"},"PeriodicalIF":1.5,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12506741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of moisture and fermentation length on the quality and digestibility of fermented concentrate using tamanu kernel cake as the main protein source through an <i>in vitro</i> study.","authors":"Dimas Hand Vidya Paradhipta, Chusnul Hanim, Ali Agus, Budi Leksono, Aziz Umroni, Sinta Maharani, Arrynda Rachma Dyasti Wardani, Zazin Mukmila","doi":"10.5455/javar.2025.l910","DOIUrl":"10.5455/javar.2025.l910","url":null,"abstract":"<p><strong>Objective: </strong>The present study aimed to evaluate the effects of moisture and fermentation length on the chemical compositions, fermentation characteristics, feed-out phase, and ruminal digestibility of fermented concentrate using tamanu kernel cake (TKC) as the main protein source.</p><p><strong>Materials and methods: </strong>The concentrate was formulated to contain 16.5% crude protein (CP) and 35% neutral detergent fiber consisting of 40% TKC, 7.60% soybean meal, 25.0% wheat pollard, 26.4% dried cassava, and 1% molasses. Those ingredients were mixed and fermented anaerobically at 5 kg into a vacuumed plastic bag with different additional sterile distilled water to reach moisture levels at 25% (MO25), 35% (MO35), and 45% (MO45). Each moisture level was incubated with different fermentation lengths consisting of 2, 7, 14, 21, and 42 days in quadruplicate. After fermentation, each silo was sub-sampled for laboratory analyses.</p><p><strong>Results: </strong>MO25 and MO35 led to higher CP with lower acid detergent fiber than MO45. In the fermentation, MO35 and MO45 generated higher (<i>p</i> < 0.05) lactate than MO25. An extended fermentation length linearly dropped (<i>p</i> < 0.05) dry matter, CP, and ether extract, but gradually increased (<i>p</i> < 0.05) ammonia-N, lactate, acetate, and the counts of lactic acid bacteria, yeast, and bacilli. After 42 days, MO25 and MO35 initiated higher (<i>p</i> < 0.05) aerobic stability. The digestibility and total volatile fatty acid (VFA) in the rumen increased (<i>p</i> < 0.05) over 2 days. However, prolonged fermentation length linearly decreased (<i>p</i> < 0.05) total VFA and methane emission without affecting rumen pH, ammonia-N, and each VFA profile.</p><p><strong>Conclusion: </strong>The application of MO35 was found to reduce nutrient loss and improve aerobic stability comparable to MO25 while achieving fermentation quality similar to MO45, and short-term fermentation, such as 2 days, could improve ruminal digestibility.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 2","pages":"433-444"},"PeriodicalIF":1.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12506699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic resistance and pathogenicity of Shiga-toxin-producing <i>Escherichia coli</i> (STEC) and non-STEC isolated from goats in the Mekong Delta, Vietnam.","authors":"Thuan Khanh Nguyen, Trung Thanh Truong, Toan Tri Nguyen, Duy Duc Tran, Thu Thi Chuong Dang, Binh Cong Tran","doi":"10.5455/javar.2025.l908","DOIUrl":"10.5455/javar.2025.l908","url":null,"abstract":"<p><strong>Objective: </strong>Our study is conducted to identify serotypes, antibiotic resistance, heavy metal resistance, and virulent genes in <i>Escherichia coli</i> isolated from goats raised in small-scale farms in some provinces of the Mekong Delta, Vietnam.</p><p><strong>Material and methods: </strong>A total of 203 <i>E</i>. <i>coli</i> isolates from goat feces were examined by PCR for serotypes (O8, O9, O25, O26, O45, O103, O146, O157, and O159), eight antibiotic-resistance genes, four heavy-metal-resistance genes, and four pathogenic genes.</p><p><strong>Results: </strong>By PCR, 20.20% of <i>E</i>. <i>coli</i> isolates belonging to serotypes O8 (6.40%), O45 (13.30%), and O159 (0.49%) were identified. Antibiotic-resistance genes were recorded at high rates in <i>E</i>. <i>coli</i> isolates, especially genes <i>blaampC</i> (98.52%), <i>tetA</i> (50.74%), <i>sulII</i> (34.48%), <i>qnrA</i> (20.69%), and <i>aadA1</i> (20.69%). Moreover, 55.67% of these <i>E</i>. <i>coli</i> isolates harbored multiple antibiotic-resistance genes. Among heavy-metal-resistance genes, the gene <i>czcD</i> encoding for resistance to cobalt, zinc, and cadmium was the most prevalent (59.11%). In addition, the most frequent virulent gene was <i>stx1</i> (15.27%), followed by gene <i>stx2</i> (6.90%), <i>eae</i>, and <i>hlyA</i> (1.48%).</p><p><strong>Conclusion: </strong>These results revealed that goats were a natural reservoir of pathogenic <i>E</i>. <i>coli</i> serotypes, which could cause severe diseases in animals and humans. Moreover, these <i>E</i>. <i>coli</i> isolates showed a high ability to resist diverse antibiotics. Thus, managing the prevalence of pathogenic <i>E</i>. <i>coli</i> is essential for protecting public health in the Mekong Delta.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 2","pages":"420-426"},"PeriodicalIF":1.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12506771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular identification of antibiotic-resistant and virulent <i>Escherichia coli and Staphylococcus aureus</i> isolated from dogs in Southern Benin.","authors":"Ayaovi Bruno Yaovi, Arpita Das, Rama N Behera, Prudencio Comlan Sossa-Minou, Vinod Singh Bisht, Monica Yadav, Ayushi Kapoor, François Dossa, Paulin Azokpota, Souaϊbou Farougou, Lamine Baba-Moussa, Kiran Ambatipudi, Philippe Sessou","doi":"10.5455/javar.2025.l907","DOIUrl":"10.5455/javar.2025.l907","url":null,"abstract":"<p><strong>Objective: </strong>Antibiotic resistance is a worldwide health challenge. Close interaction with dogs carrying antibiotic-resistant zoonotic agents poses a risk to human health. The present study aimed to characterize antibiotic-resistant <i>Escherichia coli</i> (<i>E</i>. <i>coli</i>) and <i>Staphylococcus aureus</i> (<i>S</i>. <i>aureus</i>) isolated from dogs in Southern Benin.</p><p><strong>Materials and methods: </strong>A total of 336 swabs (112 buccal, 112 nasal, and 112 rectal) from 112 dogs living in the communes of Abomey-Calavi and Cotonou were analyzed for <i>E</i>. <i>coli</i> and <i>S</i>. <i>aureus</i> presence. Bacterial isolates were tested for antibiotic (penicillins, tetracyclines, aminoglycosides, cephalosporins, sulfonamides, and macrolides) susceptibility using the disc diffusion method, and antibiotic-resistant strains were characterized by the polymerase chain reaction method.</p><p><strong>Results: </strong>A 41.07% and 20.53% of dogs harbored <i>E</i>. <i>coli</i> and <i>S</i>. <i>aureus</i>, respectively. <i>Escherichia coli</i> and <i>S</i>. <i>aureus</i> isolates showed resistance to penicillin (100% and 81.48%), tetracycline (44.64% and 59.26%), and other antimicrobials tested. <i>Escherichia coli</i> isolates harbored resistance genes <i>bla</i>TEM (63.46%), <i>tetA</i> (62.50%), and <i>strA-strB</i> (55.56%). <i>tetK</i> (100%), <i>tetM</i> (100%), and <i>bla</i>Z (82.61%) were present in <i>S</i>. <i>aureus</i> isolates. <i>Escherichia coli</i> strains harbored virulence genes <i>fimH</i> (61.54%), <i>kpsMTII</i> (26.92%), <i>fyuA</i> (19.23%), and <i>eae</i> (1.92%), whereas 20.83% of <i>S</i>. <i>aureus</i> strains harbored <i>pvl</i> and <i>fnbA</i>.</p><p><strong>Conclusion: </strong>The results of the current study reveal the urgent need for stricter controls on antibiotic use. Implementing guidelines, responsible prescribing, and increasing public awareness are crucial steps to address this problem.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 2","pages":"396-419"},"PeriodicalIF":1.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12506730/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial usage assessment and the factors associated among small-scale household dairy farms in a district of southern India.","authors":"Shwetha Prabhu, Rinila Das, Arun Kharate, Ajith M Nayak, Navya Vyas","doi":"10.5455/javar.2025.l911","DOIUrl":"10.5455/javar.2025.l911","url":null,"abstract":"<p><strong>Objective: </strong>The study was primarily conducted to assess antimicrobial usage, associated factors, and animal health management practices in small-scale household dairy farms in a district of southern India.</p><p><strong>Materials and methods: </strong>A total of 330 dairy farmers participated in the study, and single-stage cluster sampling was performed, followed by probability proportional to size sampling. A semi-structured, validated questionnaire and a checklist were used to collect the data.</p><p><strong>Results: </strong>Only a few dairy farmers knew about antimicrobials (33%) and antimicrobial resistance (AMR) (10.9%). All 330 dairy farmers were using antimicrobials. The factors that influenced dairy farmers' decisions to use antimicrobials for their cattle were veterinarian advice (100%), para-veterinarian advice (96.3%), peer influence (31.2%), and previous experience of using antimicrobials (12.4%). None of them were aware of the drug withdrawal period and followed it. Significant differences in completing the full course of antimicrobial treatment as prescribed have been observed with increasing levels of education (<i>p</i> < 0.001).</p><p><strong>Conclusion: </strong>Despite the wide usage of antimicrobials in dairy farms, there was a significant lack of knowledge among dairy farmers regarding antimicrobials and AMR. The study emphasizes the need for targeted educational interventions to improve farmers' understanding of antimicrobial use and resistance, promote responsible practices, and enhance animal health management.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 2","pages":"445-453"},"PeriodicalIF":1.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12506765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GluN2B mRNA expression and molecular sequence in the brain of pigeons (<i>Columba livia</i>).","authors":"Mohammad Rabiul Karim, Ahmed I Abo-Ahmed, Abu Raihan, Md Asif Karim Hemel, Md Alamgir Kobir, Munmun Pervin","doi":"10.5455/javar.2025.l909","DOIUrl":"10.5455/javar.2025.l909","url":null,"abstract":"<p><strong>Objectives: </strong>The current study sought to ascertain the mRNA expression and establish the complementary DNA (cDNA) sequences of pigeon brain's glutamate receptor 2B of N-methyl-D-aspartate (GluN2B) type.</p><p><strong>Material and methods: </strong>Adult pigeons (<i>Columba livia; n</i> = 8, sharing an equal number of males and females) were used. After proper anesthesia, the brain was exposed, and small pieces of cerebellum, optic tectum, thalamus, and telencephalon were collected quickly; total ribonucleic acid (RNA) was isolated, and cDNA was synthesized for PCR amplification. The ABI Prism 3100 Genetic Analyzer was used to analyze the sequences of the corresponding cDNA fragments.</p><p><strong>Results: </strong>In RT-PCR, the findings unequivocally demonstrated that the pigeon brain's cerebellum, optic tectum, thalamus, and telencephalon all expressed the mRNA for GluN2B. The cDNA sequence of pigeon GluN2B was obtained from PCR-amplified products and included 51 base pairs (bp) of the 5' untranslated region (UTR), a 4,512-bp open reading frame, and 13 bps of the 3' UTR. Pigeon GluN2B's cDNA sequencing displayed 85% identity for human GluN2B and 95% identity for chicken. The amino acid sequences encoded by the pigeon GluN2B gene shared between 85% and 97% similarity with those of humans, rats, and mice. Molecular phylogenetic analysis using the neighbor-joining method showed that pigeon GluN2B is closely related to the GluN2B proteins of these other species.</p><p><strong>Conclusion: </strong>The findings suggest that certain neurons in the pigeon brain GluN2B mRNA. They also indicate the presence of various glutamatergic networks and connections within the avian brain.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 2","pages":"427-432"},"PeriodicalIF":1.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12506696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-methanogenic effect of phytogenic extract of <i>Moringa oleifera</i> on methane mitigation through inhibition of methyl-coenzyme M reductase receptor: <i>In silico</i> study.","authors":"Muhammad Sulaiman Daulai, Indah Wijayanti, Yuli Retnani, Suzuki Toshisada","doi":"10.5455/javar.2025.l904","DOIUrl":"10.5455/javar.2025.l904","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to assess the anti-methanogenic potential of <i>Moringa oleifera</i> L. phytogenic extracts through <i>in silico</i> inhibition of the methyl-coenzyme M reductase (MCR) receptor.</p><p><strong>Materials and methods: </strong>Phytochemicals from <i>M</i>. <i>oleifera</i> were screened and compared with anti-methanogenic compounds such as 3-nitrooxypropanol and native MCR enzyme ligands (coenzyme M and coenzyme B). Molecular docking analysis was performed using AutoDock Vina on PyRx 0.8, and interactions were visualized with Discovery Studio 2024.</p><p><strong>Results: </strong>Selected phytochemicals, including pterygospermin, exhibited promising drug-likeness based on Lipinski's rule of five and absorption, distribution, metabolism, excretion, and toxicology properties. Pterygospermin demonstrated the highest binding affinity to the MCR enzyme's active site, with interactions including Pi-sulfur (Phe443), Pi-alkyl (Val482, Leu320, and Met324), Pi-Pi stacking (Phe330), and van der Waals forces (Tyr333 and Ser325).</p><p><strong>Conclusion: </strong>Pterygospermin shows potential as a competitive inhibitor of the MCR enzyme, providing a sustainable approach to mitigate methane emissions in livestock and contribute to global greenhouse gas reduction efforts.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 2","pages":"361-373"},"PeriodicalIF":1.5,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12506701/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel reassortant strain of the infectious bursal disease virus (IBDV ASPVB) from Iraqi broiler farms: A first-time molecular and histopathological investigation revealing new insights.","authors":"Ammar Dhari Abdel Fattah, Samer Sadeq Hameed","doi":"10.5455/javar.2025.l906","DOIUrl":"10.5455/javar.2025.l906","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this investigation was to identify and detect the reassortant infectious bursal disease virus (IBDV) strain from broiler farms suspected of being infected.</p><p><strong>Materials and methods: </strong>The broiler yielded 72 samples, including the spleen and bursa of Fabricius. The tissues underwent histological examination before being used in a typical PCR molecular investigation.</p><p><strong>Results: </strong>The strain was subsequently termed IBDV ASPVB. The IBDV ASPVB strain in Iraq has been identified as a novel reassortant strain based on the results of PCR, sequencing, and phylogenetic analysis of partial segments A and B. Segment A of this strain is derived from the highly pathogenic IBDV strain. In contrast, segment B is derived from other field reassortant strains. Infection with this strain might result in minor clinical symptoms but substantial damage to lymphoid organs, leading to compromised immunological responses.</p><p><strong>Conclusion: </strong>As a result of ongoing evolution, this study demonstrates that IBDV in Iraq exhibits a wide range of histological, genetic, and phenotypic variation; to our knowledge, this paper represents the first report of reassortant IBDV in Iraq.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 2","pages":"385-395"},"PeriodicalIF":1.5,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12506698/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic evaluation of different graded Holstein Friesian × Local (HF × L) crossbred breeding bulls of Bangladesh.","authors":"Md Forhad Ahmed Hridoy, Sadia Afrin Siddiqua, Doo Ho Lee, Yeong Kuk Kim, Md Shane Khoda, Monira Akter Mou, Abul Kashem Fazlul Haque Bhuiyan, Seung Hwan Lee, Mohammad Shamsul Alam Bhuiyan","doi":"10.5455/javar.2025.l901","DOIUrl":"10.5455/javar.2025.l901","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to estimate genetic parameters, breeding value, and ranking of Holstein Friesian × Local (HF × L) crossbred sires based on multi-trait selection index information.</p><p><strong>Methods: </strong>A total of 51 HF × L crossbred breeding bulls of three different genetic groups (50%HF × 50%L, 62.5%HF × 37.5%L, and 75%HF × 25%L) managed at Central Cattle Breeding and Dairy Farm (CCBDF) were evaluated based on 4,319 half-sib progeny performance data. The descriptive statistical analysis was performed using the R package. Genetic parameters were estimated using BLUPF90 by a single-trait animal model. A selection index was constructed using adjusted breeding values multiplied by variable economic weightage for each trait.</p><p><strong>Results: </strong>Genotype had significant effects on the investigated semen quality attributes (<i>p <</i> 0.05), where 75%HF × 25%L and 62.5%HF × 37.5%L crossbred genotypes both differed significantly from 50%HF × 50%L crossbreds (<i>p <</i> 0.05). In general, better productive and reproductive performances were found with the progression of HF inheritance. Daughters of 75%HF × 25%L crossbred bulls showed the highest average performance in birth weight (27.20 ± 0.09 kg), daily milk yield (8.55 ± 0.06 l), peak milk yield (10.44 ± 0.07 l), and lactation length (233.53 ± 0.85 days).The study investigated variance components of eight productive and reproductive traits such as the birth weight of calf, age at first conception, service per conception, daily milk yield, peak milk yield, lactation length, and calving interval where estimated heritability ranged from 0.09 to 0.32. Genetic correlations among the considered traits were found to be mostly weak. Among the top 20%, breeding bulls belonged to 75%HF × 25%L and 62.5%HF × 37.5%L crossbred groups.</p><p><strong>Conclusion: </strong>This study provides insightful information on the genetic evaluation of different graded bulls that could be the basis for the proven crossbred breeding bulls' selection process at CCBDF.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"12 2","pages":"334-343"},"PeriodicalIF":1.5,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12506709/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}