Journal of Advanced Veterinary and Animal Research最新文献

{"title":"Molecular identification and phylogenetic characterization of <i>Leptospira</i> spp. in domestic animals from Jordan.","authors":"Mohammad H Gharaibeh, Sawsan S Jarrah, Sameeh M Abutarbush, Farah R Al Qudsi, Ibrahim M Alzuheir","doi":"10.5455/javar.2026.m1044","DOIUrl":"10.5455/javar.2026.m1044","url":null,"abstract":"<p><p><b>Background:</b> Leptospirosis is an emerging zoonotic disease-causing substantial morbidity, mortality, and economic losses worldwide, with over 60,000 human deaths annually. Early diagnosis and molecular identification of <i>Leptospira</i> spp. are essential for effective control and prevention. In Jordan, while serological evidence exists, there is a critical lack of molecular data regarding the species circulating in animal hosts. <b>Objectives:</b> This study aimed to detect <i>Leptospira</i> infection using nested polymerase chain reaction (PCR) and to characterize the isolates molecularly through sequencing and phylogenetic analysis of the <i>16S rRNA</i> (<i>rrs</i>) gene. <b>Materials and Methods:</b> DNA was extracted from serum and urine samples collected between January 2020 and May 2021 from 82 animals suspected of leptospirosis, including 80 cattle, one goat, and one dog. Pooled urine from imported feedlot calves and urine from the goat and dog were analyzed. Positive <i>Leptospira</i> samples were identified using nested PCR targeting the <i>rrs</i> gene, followed by Sanger sequencing. Phylogenetic analysis was performed using MEGA software, and sequences were compared with reference serovars from GenBank. <b>Results:</b> Three samples (3.5%) were positive for the <i>Leptospira</i> <i>rrs</i> gene, producing the expected 299 bp amplicon. Sequencing confirmed <i>Leptospira</i> DNA in two samples-one from pooled urine of imported calves and one from a dog serum sample. The sequences were submitted to GenBank as \"Uncultured <i>Leptospira</i> spp. Canine Jordan 2020\" (OK394051) and \"Uncultured <i>Leptospira</i> spp. Cattle Jordan 2020\" (OK394045). <b>Conclusions:</b> This is the first molecular characterization of <i>Leptospira</i> spp. in domestic animals in Jordan. The findings underscore the need to include leptospirosis in differential diagnoses of febrile diseases and to enhance surveillance and preventive programs.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"13 2","pages":"420-426"},"PeriodicalIF":1.9,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13546708/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148900553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative assessment of medicinal plant, probiotic, and antibiotic supplementation on the growth performance, carcass traits, and meat quality of broiler chicken.","authors":"Momota Rani Debi, Shahnur Rahman Sohag, Piash Sikder, Md Abu Rayhan, Bapon Dey, Mohammad Al-Mamun, Md Abul Hashem, Khan Md Shaiful Islam","doi":"10.5455/javar.2026.m1052","DOIUrl":"10.5455/javar.2026.m1052","url":null,"abstract":"<p><p><b>Objectives:</b> Due to rising concerns about antimicrobial resistance to antibiotic growth promoters, the use of natural feed additives is a safer alternative in poultry production. Therefore, this study aimed to examine the impact of dietary supplementation of moringa, yeast, and antibiotics on the overall productivity of broiler chicken. <b>Materials and Methods:</b> A total of 240-day-old chicks were allocated into 4 dietary treatments (60 chicks/treatment), namely CD = Control diet, CDM = Control diet + 2.5% moringa leaf powder, CDY = Control diet + 2.5% yeast, and CDA = Control diet + 0.015% antibiotic, each having six replications (10 chicks/replication) for a period of 35 days. <b>Results:</b> The final body weight (1736 gm) was significantly (<i>p</i> < 0.05) higher in the CDA group than in the others. In feed intake, there were no significant (<i>p</i> > 0.05) differences, but FCR and digestibility were improved in the CDA and CDY groups. Bone mineralization increased in the CDM group. Treatment groups showed significantly higher dressing yield and meat yield than the control (<i>p</i> < 0.05). The cooking loss, drip loss, water-holding capacity, TBARS value, and redness of color showed better results (<i>p</i> < 0.05) in the moringa and yeast groups than the control and antibiotic groups. The meat protein content was significantly (<i>p</i> < 0.05) higher in the CDM (21.68%) and CDY (21.10%) groups compared to CDA (20.33%). Lower fat content was recorded in the CDM (1.12%) and higher in the CDA (1.67%) group. <b>Conclusions:</b> It can be concluded that the moringa or yeast-supplemented feeds are suitable options and appear to be promising choices for producing safe meat with improved nutritional quality.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"13 2","pages":"498-509"},"PeriodicalIF":1.9,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13546715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148900656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of negative dietary cation-anion difference (DCAD) diet on primiparous cow metabolomic analysis: Colostrum and milk assay.","authors":"Oscar David Aguilar-Vega, Héctor Aarón Lee-Rangel, Francisco Javier Castro-Lomelí, Ricardo Emmanuel Gaytan-Rodríguez, Anayeli Vázquez-Valladolid, Othoniel H Aragón Martínez, Mario Alejandro Mejía-Delgadillo, Rogelio Flores Ramírez","doi":"10.5455/javar.2026.m1054","DOIUrl":"10.5455/javar.2026.m1054","url":null,"abstract":"<p><p><b>Objectives:</b> This study aimed to characterize the metabolomic profile of colostrum and milk in primiparous Holstein cows subjected to a prepartum diet with a low negative dietary cation-anion difference (DCAD). <b>Materials and Methods:</b> Eight primiparous cows were randomly assigned to two treatments: (a) basal diet (<i>n</i> = 4) and (b) basal diet supplemented with 250 gm/day of anionic salts (<i>n</i> = 4), starting 21 days before parturition. Colostrum and milk samples were analyzed using gas chromatography-mass spectrometry (GC-MS). Data were log-transformed and evaluated using multivariate (PLS-DA) and univariate (Wilcoxon test with fold change) approaches in MetaboAnalyst 6.0. <b>Results:</b> A total of 34 and 31 metabolites were detected in colostrum and milk, respectively. Multivariate analysis showed a visual separation between treatments; however, model validation parameters indicated limited predictive performance. Volcano plot analysis did not identify metabolites with statistical significance (<i>p</i> < 0.05), although several compounds exhibited consistent trends in relative abundance between groups. In colostrum, fatty acids such as arachidic acid and cholesterol tended to be lower in DCAD-supplemented cows, whereas L-glutamate and dodecanedioic acid showed higher relative abundance. In milk, similar trend-level differences were observed in fatty acid profiles between treatments. <b>Conclusions:</b> Prepartum supplementation with a negative DCAD diet was associated with exploratory metabolomic shifts in colostrum and milk of primiparous cows, particularly in lipid-related compounds. These findings should be interpreted as preliminary, and further studies with larger sample sizes are required to confirm the observed patterns.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"13 2","pages":"522-529"},"PeriodicalIF":1.9,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13546712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148900687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of peste des petits ruminants virus in small ruminants from the Savannah District, Northern Ivory Coast.","authors":"Konan Ange-Sylvestre Goli, Aristide Anicet Zobo, Augustino Alfred Chengula, Melvyn Quan, Benjamin Kiffopan M'Bari, Mariam Makange, Jean Nepomuscene Hakizimana, Peter Thompson, Gerald Misinzo","doi":"10.5455/javar.2026.m1041","DOIUrl":"10.5455/javar.2026.m1041","url":null,"abstract":"<p><p><b>Objectives:</b> Peste des petits ruminants (PPR) is a highly contagious viral disease affecting sheep and goats, with significant socio-economic consequences. The disease is endemic in the Ivory Coast; molecular data on circulating strains, particularly in the northern Savannah district, remain limited. This study aimed to characterize the PPR virus (PPRV) strains circulating among sheep and goats in this area. <b>Materials and Methods:</b> A total of 355 nasal swabs were collected from unvaccinated small ruminants, regardless of clinical status, across three regions of the Savannah district (Poro, Tchologo, and Bagoué). Viral ribonucleic acid (RNA) was extracted and screened using real-time reverse transcription polymerase chain reaction (RT-qPCR) targeting the nucleoprotein (<i>N</i>) gene of PPRV, followed by conventional RT-PCR (RT-cPCR). Positive amplicons were sequenced using the Sanger method, and phylogenetic analysis was performed with PhyML and edited in MEGA X software. <b>Results:</b> Out of 355 samples analyzed, 25 (7%) tested positive by RT-qPCR, and 17 of these were amplified by RT-cPCR for nucleotide sequencing. Ten sequences were of sufficient quality for phylogenetic analysis. All sequences described in this study clustered within lineage IV of PPRV and shared high nucleotide identity (96-98%) with previously described West African PPRV strains. Phylogenetic reconstruction revealed two distinct sub-clusters, suggesting possible multiple viral introductions, likely facilitated by uncontrolled transboundary livestock movements. <b>Conclusions:</b> This study provides the first molecular evidence of PPRV circulation in the Savannah district, confirming the dominance of lineage IV. The findings underscore the need for enhanced molecular surveillance and cross-border collaboration to inform regional vaccination and eradication efforts.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"13 2","pages":"388-397"},"PeriodicalIF":1.9,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13546718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148900505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shrimp shell chitosan improves preservation quality and <i>in vitro</i> rumen digestibility and reduces methane concentration.","authors":"Rakhmad Perkasa Harahap, Yeti Rohayeti, Saitul Fakhri, Anuraga Jayanegara, Sri Suharti, Nahrowi Nahrowi","doi":"10.5455/javar.2026.m1045","DOIUrl":"10.5455/javar.2026.m1045","url":null,"abstract":"<p><p><b>Objectives:</b> This research assessed the impact of additives derived from shrimp shells, specifically chitosan, on the preservation of total mixed rations (TMR) in both anaerobic and aerobic environments, along with their effects on <i>in vitro</i> rumen digestibility, fermentation attributes, and methane production. <b>Materials and Methods:</b> A 2 × 4 factorial randomized complete block design was employed, featuring five replications. The study included two preservation conditions (anaerobic and aerobic) and four additive treatments: control, shrimp shell, chitin, and chitosan at 1% of fresh matter. After 30 days of storage, we assessed the preservation characteristics and the <i>in vitro</i> rumen fermentation variables. <b>Results:</b> Anaerobic preservation led to decreased dry matter loss, pH, and NH₃, and to increased total volatile fatty acids compared with aerobic preservation (<i>p</i> < 0.01). Chitosan showed the most beneficial preservation effect, exhibiting the least dry matter loss (3.50%) and pH (5.20), along with the highest organoleptic quality. Significant interactions between treatment and additive were observed for traits related to preservation, suggesting that the positive effects of chitosan were more pronounced under anaerobic conditions. Additionally, chitosan achieved the highest <i>in vitro</i> dry matter and organic matter digestibility (56.61% and 58.30%, respectively), along with the lowest methane concentration and cumulative gas production (<i>p</i> < 0.05). <b>Conclusions:</b> Shrimp shell-derived chitosan at 1% fresh matter effectively enhances TMR preservation and improves rumen fermentation, thereby increasing digestibility and reducing methane concentration. This indicates the potential to use shrimp shell waste as a valuable feed additive, promoting sustainable ruminant feeding and supporting circular economy practices.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"13 2","pages":"427-439"},"PeriodicalIF":1.9,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13546724/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148900561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influencing of particle size, calcination, and mineral enrichment of local calcites on productivity and economic efficiency of quails (<i>Coturnix japonica</i>).","authors":"Khalil Khalil, Dwi Ananta, Andri Andri, Ridho Kurniawan Rusli","doi":"10.5455/javar.2026.m1063","DOIUrl":"10.5455/javar.2026.m1063","url":null,"abstract":"<p><p><b>Background:</b> Small-scale quail farmers commonly use semi-self-mixed diets that may reduce mineral availability and compromise laying performance. Locally sourced calcite could provide a cheaper, more effective alternative to commercial mineral premixes. <b>Objectives:</b> This study evaluated the effects of locally formulated calcite-based mineral supplements that differed in particle size, calcination, and mineral enrichment on the productivity, physiological responses, and economic efficiency of laying quails. <b>Materials and Methods:</b> Five local calcite-based mineral formulas were produced from rock flour and oyster shell meal in raw or calcined forms, with or without mineral enrichment. A total of 432 laying quails were assigned to six dietary treatments, including a commercial mineral premix and five local mineral supplements incorporated at 5% of the basal diet for eight weeks. Productive performance, eggshell quality, blood mineral profile, physical characteristics of the minerals, and economic returns were evaluated. <b>Results:</b> Local calcite-based minerals significantly improved egg production, egg mass, feed conversion ratio, and net return compared with the commercial premix (<i>p</i> < 0.05). Calcite grit (Cag) produced the highest productivity and profitability. Its coarse particle size and greater density likely enhanced calcium retention and sustained mineral release for eggshell formation. Although calcination and mineral enrichment increased serum phosphorus levels, they did not further improve production performance or economic efficiency. <b>Conclusions:</b> Calcite grit is an effective and economically superior mineral supplement for laying quails. Physical characteristics, particularly coarse particle size and density, contributed more to productive performance than calcination or mineral enrichment.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"13 2","pages":"604-615"},"PeriodicalIF":1.9,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13546722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148900536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive pathological insights and molecular detection of caseous lymphadenitis caused by <i>Corynebacterium pseudotuberculosis</i> in slaughtered sheep, goats, and cattle.","authors":"Nazneen Sultana, Mohammad Abu Hadi Noor Ali Khan, Munmun Pervin, Sajeda Sultana, Moutuza Mostaree, Anika Tabassum, Mahmuda Islam","doi":"10.5455/javar.2026.m1037","DOIUrl":"10.5455/javar.2026.m1037","url":null,"abstract":"<p><p><b>Objectives:</b> Caseous lymphadenitis (CLA) is one of the significant economically important diseases of small ruminants caused by <i>Corynebacterium pseudotuberculosis</i>. Despite the occurrence of CLA worldwide, there are no reports of CLA in slaughtered ruminants in Bangladesh. This study was designed to investigate the pathology and molecular detection of CLA in slaughtered sheep, goats, and cattle in Bangladesh. <b>Materials and Methods:</b> A total of 102 goats, 16 sheep, and 50 cattle were examined at several slaughterhouses in Mymensingh Sadar. Mesenteric lymph nodes, lungs, liver, spleen, and kidneys from animals with suspected internal lesions were evaluated pathologically. Confirmation of <i>C. pseudotuberculosis</i> was performed by polymerase chain reaction (PCR). <b>Results:</b> Gross examination revealed enlarged mesenteric lymph nodes with multiple abscesses containing greenish-yellow caseous material. The spleen exhibited a characteristic onion-skin appearance, commonly associated with CLA. Histopathology revealed caseous necrosis, encapsulated by fibrous tissue, with infiltration of neutrophils and mononuclear cells, along with Gram-positive coccobacilli arranged in Chinese letter patterns. Similar lesions were observed in other visceral organs of goats. PCR detected <i>C. pseudotuberculosis</i> in 29.41% (30/102) goats, 6.25% (1/16) sheep, and 18% (9/50) cattle. <b>Conclusions:</b> To date, it is the first report of internal CLA in slaughtered ruminants in Bangladesh, as confirmed by pathological and molecular findings, and it highlights the importance of slaughterhouse-based surveillance for disease control.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"13 1","pages":"351-360"},"PeriodicalIF":1.9,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13197677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148015457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First insights into the physiological status and gut microbiota of free-grazing Tibetan goats in Kangding, Western China.","authors":"Jian Huang, Haiyue Xiao, Xi Liu, Wenwu Gao, Yao Pan, Yuxia Wang, Li Ma, Shengping Long, Yan Li, Jiuhong Heng","doi":"10.5455/javar.2026.m1036","DOIUrl":"10.5455/javar.2026.m1036","url":null,"abstract":"<p><p><b>Objectives:</b> This study assessed the health status, morphometric traits, and gastrointestinal microbiota of Tibetan goats reared under traditional free-range conditions in Kangding, western China, without antibiotics, anthelmintics, or vaccinations. <b>Materials and Methods:</b> Five adult Tibetan goats were assessed for body weight, linear measurements, and blood parameters. Microbial communities from six gut segments of the gastrointestinal tract were characterized using <i>16S rRNA</i> gene sequencing. To examine seasonal dynamics, rectal fecal microbiota from eight additional goats, sampled during winter and summer, were compared. <b>Results:</b> Despite fluctuating forage availability, growth traits were comparable to those reported for established meat goat breeds. Most hematological and serum biochemical values fell within normal physiological ranges, though mild deficiencies in certain minerals were observed, accompanied by geophagic behavior. The gut microbiota exhibited clear spatial structuring along the digestive tract, with Firmicutes and Bacteroidota as the dominant phyla and higher microbial richness in the hindgut. Seasonal differences were evident in the rectal fecal microbiota, with higher relative abundance of <i>Escherichia-Shigella</i> in winter and increased levels of Prevotella and the <i>Christensenellaceae_R-7_group</i> during summer. Functional predictions suggested greater nutrient transport and signaling capacity in the small intestine, whereas the hindgut showed stronger metabolic activity, particularly in fermentation-related pathways. <b>Conclusions:</b> Tibetan goats demonstrate notable physiological and microbial adaptability under extensive management. However, seasonal nutritional constraints may pose subclinical health risks. Integrating routine monitoring of physiological and microbial indicators could help safeguard animal welfare, maintain meat quality, and support the long-term sustainability of traditional pastoral systems in this region.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"13 1","pages":"340-350"},"PeriodicalIF":1.9,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13197658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148015622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The sambiloto (<i>Andrographis paniculata</i>) phytochemical as a feed additive for the prevention of <i>Eimeria</i> sp. infection in broilers.","authors":"Agustina Dwi Wijayanti, Wahyurafida Putri, Guntari Titik Mulyani, Dwi Priyowidodo, Ika Nindya Irianti","doi":"10.5455/javar.2026.m1035","DOIUrl":"10.5455/javar.2026.m1035","url":null,"abstract":"<p><p><b>Objectives:</b> This study aims to investigate the effectiveness of feeds containing sambiloto extracted in water (SWE) or ethanol (SEE) in improving the performance of broilers infected with <i>Eimeria</i> sp. <b>Material and Methods:</b> A total of 80 ROSS 308 strain day-old chicks were divided into 4 groups: groups FSWE and FSEE (chickens infected with <i>Eimeria</i> sp. and fed with basal feed containing SWE and SEE, respectively), group F⁺ (chickens infected and fed basal feed), and group F^- (chickens not infected and fed basal feed). Infection was performed on day 14 by oral administration at a dose of 3×10⁴ oocysts for every chick. Body weight gain, feed conversion ratio (FCR), and survival rate were calculated during the treatment period. Anticoccidial efficacy variables were measured based on the oocysts per gram (OPG), oocyst ratio, oocyst value, and lesion scoring. Data were analyzed using one-way ANOVA and the Kruskal-Wallis test. <b>Results:</b> The performance study results indicated that the highest body weight was achieved by the FSWE, and an ideal FCR was obtained from the FSEE, respectively. The lowest oocyst count was obtained in FSWE (<i>p</i> < 0.05), and the highest survival rate was observed in FSEE. <b>Conclusions:</b> The study concluded that SWE and SEE have anticoccidial efficacy in broilers and offer benefits as feed additives in preventing <i>Eimeria</i> sp. infections.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"13 1","pages":"332-339"},"PeriodicalIF":1.9,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13222458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148143159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geometric morphometric analysis of wing size and shape variation in <i>Aedeomyia catasticta</i> (Diptera: Culicidae) populations across different regions of Thailand.","authors":"Tanawat Chaiphongpachara, Tawee Saiwichai, Sedthapong Laojun","doi":"10.5455/javar.2026.m1029","DOIUrl":"10.5455/javar.2026.m1029","url":null,"abstract":"<p><p><b>Objectives:</b> <i>Aedeomyia catasticta</i> is a mosquito species with relatively limited biological information. Therefore, this study aims to examine the geographical variation of wing shape in <i>A. catasticta</i> populations from different regions of Thailand using a landmark-based geometric morphometric approach. <b>Materials and Methods:</b> Samples of <i>A. catasticta</i> were collected from each of four regions of Thailand, each with distinct geographical and ecological characteristics: Eastern, Northeastern, Southern, and Western. Wing size was estimated by comparing centroid size (CS) values between populations. Differences in wing shape were assessed using the Mahalanobis distance, and statistical significance was tested using 10,000 permutations. <b>Results:</b> Statistical analyses of wing size indicated significant differences in wing CS among various pairs of populations across different regions, particularly between eastern and western, eastern and southern, and northeastern and western populations (<i>p</i> < 0.05). Statistical comparisons of wing shapes also demonstrated that wing shape varied significantly across all four regions (<i>p</i> < 0.05). <b>Conclusions:</b> The wing CS and shape of <i>A. catasticta</i> populations from different regions of Thailand may have been influenced by regional environmental factors, as indicated by geometric morphometric analyses. These findings improve understanding of regional morphological variation in this understudied mosquito species.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"13 1","pages":"256-264"},"PeriodicalIF":1.9,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13197667/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148015577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}