Effect of interferon on broilers' fecal microbiome composition.

IF 1.5 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Advanced Veterinary and Animal Research Pub Date : 2025-05-16 eCollection Date: 2025-06-01 DOI:10.5455/javar.2025.l914

Inna Burakova, Yuliya Smirnova, Mariya Gryaznova, Polina Morozova, Vyacheslav Kotarev, Ludmila Lyadova, Nadezhda Ivanova, Larisa Denisenko, Mikhail Syromyatnikov

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Abstract

Objective: The purpose of our study was to investigate the effect of chicken interferon on the intestinal microbiota of broiler chickens.

Materials and methods: The study used next-generation sequencing on the Ion Torrent pragmatic general multicast platform to target the V3 16S ribosomal ribonucleic acid hypervariable region gene, allowing us to analyze in detail changes in the composition of the broiler chicken microbiome.

Results: Forty-one bacterial genera were identified in the studied groups of broilers. The highest abundance in both groups was observed for Lactobacillus, which was 31.08% ± 6.52 in the control group and 36.08% ± 7.25 in the interferon group. There was no clustering between the microbiome communities of the groups studied. We found a decrease or complete absence of Escherichia-Shigella, Eubacterium fissicatena group, Lachnospiraceae CHKCI001, and Pediococcus in the interferon-treated broiler group compared to the control group and an increase in the number of genera Ruminococcaceae CAG-352 and Turicibacter in the interferon group.

Conclusion: A decrease in E.-Shigella may indicate normalization of the intestinal microbiota of broilers, and it can also be concluded that the introduction of interferon helps to suppress opportunistic bacteria. In the interferon group, a sharp increase in the number of Turicibacter was observed. Representatives of this genus are among the most common members in the intestines of broilers.

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干扰素对肉鸡粪便微生物组成的影响。

目的：研究鸡干扰素对肉鸡肠道菌群的影响。材料与方法：本研究利用Ion Torrent实用通用多播平台上的下一代测序技术，针对V3 16S核糖体核糖核酸高变区基因，详细分析肉鸡微生物组组成的变化。结果：在所研究的肉鸡群体中鉴定出41个细菌属。两组中乳杆菌丰度最高，对照组为31.08%±6.52，干扰素组为36.08%±7.25。研究群体的微生物群落之间没有聚类。我们发现，与对照组相比，干扰素处理的肉鸡组中志贺氏杆菌、裂裂真杆菌组、毛杆菌科CHKCI001和Pediococcus的数量减少或完全不存在，而干扰素组中瘤胃球菌科CAG-352和Turicibacter的数量增加。结论：志贺氏杆菌的减少可能表明肉仔鸡肠道菌群的正常化，干扰素的引入有助于抑制机会菌。在干扰素组，观察到Turicibacter的数量急剧增加。本属的代表是肉鸡肠道中最常见的成员之一。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Advanced Veterinary and Animal Research Veterinary-Veterinary (all)

CiteScore

2.70

自引率

7.10%

发文量

审稿时长

6 weeks

期刊介绍： Journal of Advanced Veterinary and Animal Research (JAVAR) - is an open access, international, peer-reviewed, quarterly, highly-indexed scientific journal publishing original research findings and reviews on all aspects of veterinary and animal sciences. Basic and applied researches on- - Anatomy & histology - Animal health economics - Animal nutrition - Animal reproduction - Animal science - Antimicrobial resistance (AMR) - Biochemistry - Biotechnology - Dairy science - Epidemiology - Food hygiene and technology - Genetics and breeding - Immunology - Microbiology - Parasitology - Pathology - Pharmacology & toxicology - Physiology - Poultry science - Preventive veterinary medicine - Public health - Surgery & obstetrics - Veterinary extension studies - Wildlife & aquatic medicine - Zoo animal medicine.