Japanese Journal of Oral Biology最新文献_第6页

Decrease in TIMP-1 Level in Saliva from Patients with Taste Disorder 味觉障碍患者唾液中TIMP-1水平的降低

Japanese Journal of Oral Biology Pub Date : 2001-12-20 DOI: 10.2330/JORALBIOSCI1965.43.700

A. Igarashi, M. Monya, S. Nomura

引用次数: 2

Swelling of Collagen Fiber by Guanidine Hydrochloride and Its Calcification. 盐酸胍对胶原纤维的溶胀及其钙化作用。

Japanese Journal of Oral Biology Pub Date : 2001-12-20 DOI: 10.2330/JORALBIOSCI1965.43.653

Ki-ichiro Tanaka, T. Ono, T. Kobayakawa

引用次数: 0

Histological Changes in the Rat Submandibular Gland Following Ligation and Removal of the Main Excretory Duct 结扎和切除主要排泄管后大鼠颌下腺的组织学变化

Japanese Journal of Oral Biology Pub Date : 2001-12-20 DOI: 10.2330/JORALBIOSCI1965.43.676

Hideo Yagishita, M. Uchida, S. Aiyama

引用次数: 5

Analysis of Cell Death in Meckel's Chondrocytes In Vitro Induced by Heat-Shock Treatment 热休克诱导体外梅克尔软骨细胞死亡的研究

Japanese Journal of Oral Biology Pub Date : 2001-08-20 DOI: 10.2330/JORALBIOSCI1965.43.370

M. Kubo, K. Ishizeki

{"title":"Analysis of Cell Death in Meckel's Chondrocytes In Vitro Induced by Heat-Shock Treatment","authors":"M. Kubo, K. Ishizeki","doi":"10.2330/JORALBIOSCI1965.43.370","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.370","url":null,"abstract":"To clarify whether in vitro cell death in Meckel's chondrocytes induced by heat-stress treatment involves apoptosis or necrosis, we performed morphological examinations using immunohistochemistry including BrdU-incorporation, the TUNEL method, and light and electron microscopy. Cell death was induced in Meckel's chondrocytes isolated enzymatically from 17-day embryonic mice, by exposure to temperatures 40-60°C. On exposure t0 sublethal temperatures of 40-45°C, the incorporation of BrdU was facilitated, and TUNEL-positive apoptotic cells appeared with high frequency. At the ultrastructural level, apoptotic cell death was characterized by chromatin condensation, nuclear segmentation, and the formation of apoptotic bodies. In contrast, cell death induced by lethal temperature (50-60°C) presented decreases in TUNEL-positive cells and cell incorporating BrdU. High temperature-treated cells showed apparent disruption of the membrane of cell organelles. Immunostaining for heat shock proteins (HSP) -27 and-70 revealed that these proteins are secreted continuously at lower temperatures, but have a tendency toward decrease on exposure to lethal temperatures. Our results suggest that Meckel's chondrocytes demonstrate apoptosis at sublethal temperatures, but undergo necrosis at lethal temperatures.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"72 2 1","pages":"370-383"},"PeriodicalIF":0.0,"publicationDate":"2001-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84719696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Bipartite Action of Parathyroid Hormone-related Peptide on Cartilage 甲状旁腺激素相关肽对软骨的两部分作用

Japanese Journal of Oral Biology Pub Date : 2001-08-20 DOI: 10.2330/JORALBIOSCI1965.43.360

N. Amizuka

{"title":"The Bipartite Action of Parathyroid Hormone-related Peptide on Cartilage","authors":"N. Amizuka","doi":"10.2330/JORALBIOSCI1965.43.360","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.360","url":null,"abstract":"軟骨の研究は,骨代謝研究のなかでも発生生物学的なアプローチの視点から急速に進歩してきた領域の1つである。軟骨細胞の分化増殖,さらに軟骨内骨化に対する調節因子の研究は,基礎的な細胞生物学ばかりでなく先天異常を含めた遺伝子疾患の解明にも及び,その必要性が再認識されている(図1)。そのなかで副甲状腺ホルモン関連ペプチド(parathyroid hormone-related peptide: PTHrP)は,軟骨の分化増殖に最も重要な因子であり,多くの研究者によってその詳細が明らかにされている。PTHrPについては,すでに1950年代にAlbright が,悪性腫瘍から産生される副甲状腺ホルモン(PTH) 様の物質でありhumoral hypercalcemia of malignancy の高カルシウム血症の起因物質としてその存在を提唱していた。しかしながら,実際にそのアミノ酸・遺伝子配列の同定が明らかにされたのは1980年代後半になってからである1-3)。Albrightの予想は見事に的中しており, PTHrPはN末端アミノ酸がPTHのそれと高い相同配列を示すため,PTHと共有のPTH/PTHrPレセプターに結合することで副甲状腺機能亢進症様の活性を示すことが知られている。このような歴史的な歩みから, PTHrPは病理学的因子として扱われており,現在でも悪性腫瘍に併発する高カルシウム血症ならびに腫瘍細胞の増殖に及ぼすPTHrPの作用などが精力的に検討されている。ところがPTHrPは,oncoproteinとしての反面,胎生期のさまざまな正常組織で産生されていることから個体発生や器官形成の局所因子として解釈されるようになってきた。PTHrPは胎生期の哺乳類の多くの組","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"42 1","pages":"360-369"},"PeriodicalIF":0.0,"publicationDate":"2001-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85001070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms for Calcium Signaling and Fluid and Electrolytes Secretion in Salivary Cells 唾液细胞中钙信号和液体及电解质分泌的机制

Japanese Journal of Oral Biology Pub Date : 2001-08-20 DOI: 10.2330/JORALBIOSCI1965.43.343

A. Tanimura

{"title":"Mechanisms for Calcium Signaling and Fluid and Electrolytes Secretion in Salivary Cells","authors":"A. Tanimura","doi":"10.2330/JORALBIOSCI1965.43.343","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.343","url":null,"abstract":"唾液分泌は主にムスカリン受容体やアドレナリン受容体によって調節されている。多くの臓器では交感神経と副交感神経がアクセルとブレーキのように拮抗的な働きをしているのに対し,唾液腺では車の両輪のように協調して水分泌とタンパク質分泌という2つの分泌を調節している(図1)。唾液の水成分はphospholipase C (PLC) と連関する αアドレナリン受容体(α 受容体)やムスカリン受容体を介し,アミラーゼやムチンなどのタンパク質成分はadenylate cyclaseと連関するβアドレナリン受容体(β 受容体)を介して分泌が惹起される1)。そのほかに唾液腺のタンパク質分泌に関与するvasoactive intestinal peptide (VIP)や水分泌に関与するサブスタンスPといった生理活性物質がよく知られている1)。また最近では,プリン受容体やPAR (protease activated receptor)受容体が唾液腺細胞に存在することが報告されている2,3)。唾液中のタンパク質成分と水・電解質とでは分泌の様式がまったく異なる。水分泌はイオン・チャンネルやイオン輸送体を介してNa+, K+, Cl-などの電解質が腺腔内へ分泌され,その結果生じる浸透圧勾配によって水が腺腔内へ移動する現象である4)。一方,タンパク質分泌は腺房細胞の分泌顆粒と細胞膜の融合により,顆粒内に蓄えられたタンパク質が細胞外へ放出される開口分泌である。受容体刺激によりPLCが活性化されると,膜のリン脂質であるホスファチジルイノシトール二リン酸からジアシルグリセロール(DG)とイノシトール三リン酸(IP3) が生成される。DGはprotein kinase C (PKC)の活性","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"1 1","pages":"343-352"},"PeriodicalIF":0.0,"publicationDate":"2001-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89776845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene Expression of Bovine Dental Pulp Cells In Vitro during Odontoblast-like Cell Differentiation 牛牙髓细胞在成牙细胞样细胞分化过程中的基因表达

Japanese Journal of Oral Biology Pub Date : 2001-08-20 DOI: 10.2330/JORALBIOSCI1965.43.402

S. Fujiwara, K. Imai, K. Sanada

引用次数: 1

Mechanistic Understanding of the Stage-specific Accumulation of Magnesium Ions in Developing Enamel: Simulation of Coupling Events at the Crystal/Solution Interface in an in vitro Precipitation Model 镁离子在牙釉质发育阶段特异性积累的机制理解:体外沉淀模型中晶体/溶液界面耦合事件的模拟

Japanese Journal of Oral Biology Pub Date : 2001-08-20 DOI: 10.2330/JORALBIOSCI1965.43.424

Y. Taya, Y. Soeno, Kaori Sato, Satoshi Kurata, T. Aoba

{"title":"Mechanistic Understanding of the Stage-specific Accumulation of Magnesium Ions in Developing Enamel: Simulation of Coupling Events at the Crystal/Solution Interface in an in vitro Precipitation Model","authors":"Y. Taya, Y. Soeno, Kaori Sato, Satoshi Kurata, T. Aoba","doi":"10.2330/JORALBIOSCI1965.43.424","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.424","url":null,"abstract":"The present study investigated the mechanism of stage-specific Mg incorporation during enamel formation. To this end, a series of in vitro experiments were conducted using a precipitation model with a dialysis chamber. Synthetic hydroxyapatite and pig enamel proteins were used as seeds and regulators for precipitation, respectively. The results showed that the accumulation of Mg ion on and into apatite crystals was highly dependent on solution composition (Ca concentrations and the supersaturation levels) and protein coverages of the crystals. Fluoride at low concentrations efficiently promoted Mg intake into apatite crystals due to the accelerating effect on precipitation kinetics. Trypsin, used as a prototype of enamel resident proteases, also provided an increasing effect on Mg intake due to degradation and removal of proteins associated with crystals. From the data obtained, it is evident that stage-specific Mg incorporation during enamel formation is related to the multiple kinetic and thermodynamic aspects characteristic for enamel mineralization, namely, fluid composition and the competitive adsorption between Ca and Mg ions onto apatite surface, and accumulation of proteinaceous inhibitors of crystal growth and their removal after enzymatic degradation.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"65 1","pages":"424-433"},"PeriodicalIF":0.0,"publicationDate":"2001-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83373837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Stoichiometry and Thermodynamic Solubility Properties of Dentin Mineral of Human Permanent and Deciduous Teeth 恒牙和乳牙牙本质矿物的化学计量学和热力学溶解度

Japanese Journal of Oral Biology Pub Date : 2001-08-20 DOI: 10.2330/JORALBIOSCI1965.43.410

H. Komatsu, Kaori Sato, T. Aoba

{"title":"Stoichiometry and Thermodynamic Solubility Properties of Dentin Mineral of Human Permanent and Deciduous Teeth","authors":"H. Komatsu, Kaori Sato, T. Aoba","doi":"10.2330/JORALBIOSCI1965.43.410","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.410","url":null,"abstract":"Acquisition of substantial amounts of CO32-, HPO42- and other impurities is universally encountered in bioapatites from mammals. To date, however, there is a paucity of information about the stoichiometry and, on that basis, solubility product of dentin mineral. In this report, we summarize our experimental approach toward the determination of the stoichiometry model and thermodynamic solubility products of human dentin apatites. Dentin samples were collected separately from the coronal portion of permanent premolars (extracted for orthodontic reasons) and exfoliated deciduous teeth. Both pooled materials were pulverized with an agate-mortar, and then plasma-ashed at approx. 60°C to remove the organic matter. The stoichiometry model selected for these crystals was (Ca) 5-x (Mg) q (Na) u (HPO4) v (CO3) w (PO4) 3-y (OH) 1-z. These stoichiometric coefficients were analytically determined by assessing separately the labile or surface pools of the ionic species on the crystal surface and their stable pools in the bulk crystal lattice. The dentin mineral was equilibrated in dilute phosphoric solutions (100mg/100ml) at 25°C for a maximum of 28 days under constant partial pressures of CO2. The results showed that there are appreciable differences in the crystal stoichiometry between the permanent and deciduous dentin mineral and that the solubility product constants (KDN) were 4.11×10-45 for permanent dentin and 1.74×10-43 for deciduous dentin, which were determined under 1.8% CO2/N2. Notably, the solubility properties of the dentin mineral remained relatively constant in the range of 1.0 through 3.3% CO2/N2, whereas the discrete solubility data were obtained below and above the corresponding partial pressure of CO2.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"233 1","pages":"410-423"},"PeriodicalIF":0.0,"publicationDate":"2001-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75570609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Effects of the Posterior Margin of the Denture on the Functional Properties of Swallowing 义齿后缘对吞咽功能特性的影响

Japanese Journal of Oral Biology Pub Date : 2001-08-20 DOI: 10.2330/JORALBIOSCI1965.43.384

K. Hiraba, Yoshinori Desaki, Nobuhiro Hasegawa, Norie Nozaki, T. Ishida, Yoshinobu Tanaka

引用次数: 0