Mechanistic Understanding of the Stage-specific Accumulation of Magnesium Ions in Developing Enamel: Simulation of Coupling Events at the Crystal/Solution Interface in an in vitro Precipitation Model

Y. Taya, Y. Soeno, Kaori Sato, Satoshi Kurata, T. Aoba
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引用次数: 2

Abstract

The present study investigated the mechanism of stage-specific Mg incorporation during enamel formation. To this end, a series of in vitro experiments were conducted using a precipitation model with a dialysis chamber. Synthetic hydroxyapatite and pig enamel proteins were used as seeds and regulators for precipitation, respectively. The results showed that the accumulation of Mg ion on and into apatite crystals was highly dependent on solution composition (Ca concentrations and the supersaturation levels) and protein coverages of the crystals. Fluoride at low concentrations efficiently promoted Mg intake into apatite crystals due to the accelerating effect on precipitation kinetics. Trypsin, used as a prototype of enamel resident proteases, also provided an increasing effect on Mg intake due to degradation and removal of proteins associated with crystals. From the data obtained, it is evident that stage-specific Mg incorporation during enamel formation is related to the multiple kinetic and thermodynamic aspects characteristic for enamel mineralization, namely, fluid composition and the competitive adsorption between Ca and Mg ions onto apatite surface, and accumulation of proteinaceous inhibitors of crystal growth and their removal after enzymatic degradation.
镁离子在牙釉质发育阶段特异性积累的机制理解:体外沉淀模型中晶体/溶液界面耦合事件的模拟
本研究探讨了牙釉质形成过程中特定阶段Mg的掺入机制。为此,采用带透析室的沉淀模型进行了一系列体外实验。合成羟基磷灰石和猪牙釉质蛋白分别作为沉淀的种子和调节因子。结果表明,镁离子在磷灰石晶体上和晶体内的积累高度依赖于溶液组成(钙浓度和过饱和水平)和晶体的蛋白质覆盖率。低浓度氟化物通过对沉淀动力学的加速作用,有效地促进了镁进入磷灰石晶体。胰蛋白酶作为牙釉质常驻蛋白酶的原型,由于与晶体相关的蛋白质的降解和去除,也对镁的摄入量产生了越来越大的影响。从获得的数据可以看出,釉质形成过程中特定阶段的Mg结合与釉质矿化的多个动力学和热力学方面的特征有关,即流体组成和Ca和Mg离子在磷灰石表面的竞争性吸附,以及晶体生长的蛋白质抑制剂的积累和酶降解后的去除。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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