Japanese Journal of Oral Biology最新文献_第5页

The Effect of Streptozotocin-induced Diabetes on Bone Tissue around Endosseous Implants after Osseointegration 链脲佐菌素诱导的糖尿病对骨融合后骨内种植体周围骨组织的影响

Japanese Journal of Oral Biology Pub Date : 2002-02-20 DOI: 10.2330/JORALBIOSCI1965.44.19

Toshitake Obata, A. Yokoyama, Satoru Yamamoto, T. Kawasaki, Mitsutaka Oda, T. Iizuka, T. Kohgo

引用次数: 1

Thermodynamic and Kinetic Interpretation of Enamel Mineralization Octacalcium Phosphate (OCP) as an Inevitable Intermediate Phase 八磷酸钙(OCP)作为牙釉质矿化不可避免的中间相的热力学和动力学解释

Japanese Journal of Oral Biology Pub Date : 2002-02-20 DOI: 10.2330/JORALBIOSCI1965.44.48

T. Hiratsuka, Kaori Sato, T. Aoba

{"title":"Thermodynamic and Kinetic Interpretation of Enamel Mineralization Octacalcium Phosphate (OCP) as an Inevitable Intermediate Phase","authors":"T. Hiratsuka, Kaori Sato, T. Aoba","doi":"10.2330/JORALBIOSCI1965.44.48","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.48","url":null,"abstract":"Much attention has been given to the growth mechanism of enamel mineral by which the unique features (i. e., morphology, size and alignment) of enamel crystallites in mammals are achieved. In this paper, we report our experimental and theoretical approaches to a long-debated and unsettled issue, i. e., the involvement and role of an acidic precursor in enamel mineralization. Analysis of nucleation events, which were induced in neutral media at 37°C and 1.8% partial CO2 pressure, substantiated the involvement of octacalcium phosphate (OCP) in precipitation reactions. We also developed a new crystal growth model using miniaturized columns in tandem, in which OCP itself was proved to be equally competent, in comparison with hydroxyapatite (HA), as a template for crystal precipitation and as an adsorbent for interaction with enamel proteins. Simulation of the precipitation process taking place in situ with calcium transport (its increment) into enamel fluid also allowed us to predict that OCP is more kinetically favorable than HA, if Ca ions are supplied through the cellular activity of ameloblasts. The results obtained support the theory that OCP is involved as an inevitable intermediate phase to yield large enamel carbonatoapatite during amelogenesis.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"29 1","pages":"48-65"},"PeriodicalIF":0.0,"publicationDate":"2002-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82291207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Influence of Occlusal Hypofunction Induced by Opposed Tooth Loss on Periodontal Mechanoreceptors in Rat Molars 拮抗牙缺失所致咬合功能减退对大鼠磨牙牙周机械感受器的影响

Japanese Journal of Oral Biology Pub Date : 2002-02-20 DOI: 10.2330/JORALBIOSCI1965.44.66

Y. Seki, N. Ishii, K. Toda, K. Soma

{"title":"Influence of Occlusal Hypofunction Induced by Opposed Tooth Loss on Periodontal Mechanoreceptors in Rat Molars","authors":"Y. Seki, N. Ishii, K. Toda, K. Soma","doi":"10.2330/JORALBIOSCI1965.44.66","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.66","url":null,"abstract":"It has been critical in clinical dentistry to understand the response properties of periodontal mechanoreceptors (PMRs) when the occlusal condition was changed, because the PMRs are important sensory receptors controlling jaw movement. Therefore, we investigated the effect of opposed tooth loss on the response properties of PMRs in rat molars. All mandibular molars in an in vitro jaw-nerve preparation, made from a rat without opposed teeth, were carefully extracted before electrophysiological recordings. Single-unit discharges evoked by direct mechanical stimuli with von Frey hairs applied to the PMRs were recorded from the inferior alveolar nerve on day 3, and 1, 2, 4, 8, and 12 weeks after opposed tooth extraction. The following results were obtained; (1) in both the 3-day and 1-week post-extraction groups, the von Frey threshold value was significantly lower, although in the 12-week group, it was significantly higher than that in the control group and (2) in the 3-day to 8-week groups, the conduction velocity of nerve fibers innervating PMRs was significantly slower than that in the control group. These results showed that sustained functional changes could be clearly elicited in PMRs by occlusal hypofunction, suggesting that these changes may have critical influences on both jaw reflexes and masticatory movements.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"1 1","pages":"66-74"},"PeriodicalIF":0.0,"publicationDate":"2002-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89668746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Genotyping of Heterogenous Osteopetrotic Mice Using Microsatellite Markers 利用微卫星标记对异种骨变性小鼠进行基因分型

Japanese Journal of Oral Biology Pub Date : 2002-02-20 DOI: 10.2330/JORALBIOSCI1965.44.1

Y. Asada, Yukie Sato, Takehiko Shimizu, T. Sakae, Hirotsugu Yamamoto, T. Maeda

{"title":"Genotyping of Heterogenous Osteopetrotic Mice Using Microsatellite Markers","authors":"Y. Asada, Yukie Sato, Takehiko Shimizu, T. Sakae, Hirotsugu Yamamoto, T. Maeda","doi":"10.2330/JORALBIOSCI1965.44.1","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.1","url":null,"abstract":"The aim of this study was to elucidate the three genotypes (op/op, op/+ and +/+) of heterogenous osteopetrotic mice using microsatellite markers. The genotyping analysis was performed on genetic crosses using B6C3Fe-op/+ and A/WySnJ mice. In this study, we used five MIT markers, D3Mit11, 75, 101, 102 and 189 that are closely linked to the op locus and which segregate polymorphic variants in mating between B6C3Fe-op/+ and A/WySnJ mice, and the homozygous op/op F3 mice were obtained by F2 mice mating. From the results of F3 genotyping, it was possible to determine the genotype of the op gene without a complicated molecular process. Histopathological studies revealed that the F3-op/op mice showed similar femur bone structures to those of B6C3Fe-op/op mice. Based on these findings, it is suggested that genotyping of heterogenous osteopetrotic mice using microsatellite markers is a powerful technique for investigating the mechanism of osteoclast development and genetic studies of osteopetrosis. 抄録:大理石骨病交雑系モデルマウスを用い,マイクロサテライトマーカーによる遺伝子型を検出することで op遺伝子の3つの遺伝子型(op/op, op/+, +/+)を判定できるか否かを目的とし本実験を行った。B6C3Feop/+マウスとA/WySnJマウスとの交配より得られたすべてのF2マウスの遺伝子型をマイクロサテライトマーカーにて判定したところ,すべてのマウスで表現型(切歯の萌出の有無)と一致していた。さらに,肉眼的には+/+と判別困難なヘテロ型(op/+)マウスをマイクロサテライトマーカーより判定し,選抜したF2ヘテロ型マウス雌雄を交配したところ,F3マウスにおいてop/opマウスを作成することができた。また,組織学的所見より従来のopマウスとF3-op/opマウスでは大腿骨における形態学的特徴が類似していた。以上の結果より,大理石骨病交雑系モデルマウスはマイクロサテライトマーカーを用いることで簡便に遺伝子型を判定できることが明らかとなり,破骨細胞の分化に対するメカニズム解明ならびに大理石骨病の遺伝学的研究に有用であることが","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"22 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2002-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91545338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Adenovirus E4orf6 Suppresses the Apoptotic Activity by Bcl-2 Family Protein, BNIP 3 腺病毒E4orf6通过Bcl-2家族蛋白bnip3抑制细胞凋亡活性

Japanese Journal of Oral Biology Pub Date : 2002-02-20 DOI: 10.2330/JORALBIOSCI1965.44.40

Mariko Aoyagi, F. Higashino, M. Yasuda, T. Kohgo, H. Sano, M. Shindoh

{"title":"Adenovirus E4orf6 Suppresses the Apoptotic Activity by Bcl-2 Family Protein, BNIP 3","authors":"Mariko Aoyagi, F. Higashino, M. Yasuda, T. Kohgo, H. Sano, M. Shindoh","doi":"10.2330/JORALBIOSCI1965.44.40","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.40","url":null,"abstract":"E4orf6はアデノウイルスの新しいウイルスがん遺伝子でE4orf6が発現しているがん細胞はヌードマウスに造腫瘍性があるため, E4orf6はがん細胞の悪性化にかかわっていると考えられている. この機構にはがん抑制遺伝子産物p53とその関連タンパクp73にE4orf6が結合し, それらの機能を抑制することが重要であることをわれわれは報告してきた.今回われわれは, アポトーシス関連タンパクに対するE4orf6の影響について検索し, Bcl-2ファミリーのBNIP 3のアポトーシス誘導能をE4orf6が抑制することを見いだした. E4orf6は, N末端側にNuclear ExportSignal (NES) をコードする領域をもっている. E4orf6とBNIP 3を293細胞に遺伝子導入し, レーザー共焦点顕微鏡により観察したところ, E4orf6は細胞質中でBNIP 3のミトコンドリアへの局在を変化させることが認められた. さらに, BNIP 3との結合にはNESを含むがんの悪性化に重要な役割を果たす領域が必要であった. これらの結果は, E4orf6はBNIP 3をターゲットにして細胞中で相互作用し細胞を悪性化することを示唆している.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"28 1","pages":"40-47"},"PeriodicalIF":0.0,"publicationDate":"2002-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81950388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-dimensional Observation of the Distribution of the Inferior Alveolar Artery Using Micro-CT 显微ct对下肺泡动脉分布的三维观察

Japanese Journal of Oral Biology Pub Date : 2002-02-20 DOI: 10.2330/JORALBIOSCI1965.44.29

Y. Kitano, T. Hara, Y. Ide

{"title":"Three-dimensional Observation of the Distribution of the Inferior Alveolar Artery Using Micro-CT","authors":"Y. Kitano, T. Hara, Y. Ide","doi":"10.2330/JORALBIOSCI1965.44.29","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.29","url":null,"abstract":"The purpose of this study was to clarify the distribution of the inferior alveolar artery (IAA) and its dental branches in the mandibular canal and surrounding tissues in both dentulous and edent ulous mandibles using a Micro-CT system. Twenty-six sides of 18 mandibles from Japanese cadavers were examined . Contrast medium was injected into the maxillary artery of the examined specimens to obtain the Micro-CT and 3-D reconstruction images . Maximum vertical length and horizontal breadth of the mandibular canal were measured to determi ne the position of IAA in the canal. The angle of the dental branches against IAA and the inside diameter of IAA were also examined. The vertical position of IAA in the molar area was higher in the edentulous than that in the dent ulous mandible. There was, however, no significant difference in the horizontal position . The retromolar ramus , i n which the dental branches were distributed at the retromolar region , was usually observed to run toward the buccal just after separating from IAA. The inside diameter of IAA was smaller in the edentulous tha n that in the dentulous mandible. These results suggest that positional change of IAA in the mandibular canal and decrease of inside diameter may be caused by absorption of alveolar bone following tooth loss and subsequently morphological change of the mandibular canal. However, the running direction of the retromolar ramus did not change . 抄録:有歯顎と無歯顎における下歯槽動脈の走行形態および歯枝の分岐形態を把握することを目的として, Micro-CTを用いた三次元的観察および解析を行った。試料として顎動脈より造影剤の注入を行った日本人成人遺体18体26側を用いた。下顎管を基準とした下歯槽動脈の垂直的位置,水平的な位置,血管内径および下歯槽動脈を基準とした歯枝の分岐する角度の計測を行ったところ,以下の結果を得た。下顎管内部における下歯槽動〒261-8502 千葉県千葉市美浜区真砂1-2-2 30 歯基礎誌44: 29-39, 2002. 脈は,無歯顎になると垂直的には有歯顎と比較して相対的に上方に位置したが,水平的には差が認められなかった。血管内径は無歯顎になると細くなる傾向が認められた。歯枝は歯牙の喪失により分岐する数の減少がみられたが,臼後領域に向かう臼後枝は変化が認められなかった。また,その臼後枝は頬側方向に分岐していた。これらは,歯牙喪失による歯槽骨の吸収,それに伴う内部構造,特に下顎管の構造の変化が大きく関与していた。","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"1 1","pages":"29-39"},"PeriodicalIF":0.0,"publicationDate":"2002-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87465213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Changes in Muscle Fiber Size and Fiber Type Composition of the Masseter Muscle Induced by Long-term Feeding of a Fine-grained Diet to Rabbits 长期饲喂细粒饲粮对家兔咬肌肌纤维大小和纤维类型组成的影响

Japanese Journal of Oral Biology Pub Date : 2002-02-20 DOI: 10.2330/JORALBIOSCI1965.44.7

K. Ito, K. Hiraba, T. Negoro, M. Mizutani, Takumi Morita, T. Fujiwara, S. Goto, N. Ohno

{"title":"Changes in Muscle Fiber Size and Fiber Type Composition of the Masseter Muscle Induced by Long-term Feeding of a Fine-grained Diet to Rabbits","authors":"K. Ito, K. Hiraba, T. Negoro, M. Mizutani, Takumi Morita, T. Fujiwara, S. Goto, N. Ohno","doi":"10.2330/JORALBIOSCI1965.44.7","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.7","url":null,"abstract":"ウサギにおいて粉末飼料飼育 (離乳直後5週齢から成獣時18週齢まで) が咬筋筋線維に及ぼす影響をタイプI, IIA, IIBの3種類についてミオシンATPaseならびにSDH染色法にて検討した. その結果, 実験群の筋線維の直径は対照群 (固形飼料飼育) と比べて, タイプI線維は43.9 (対照群, n=1, 809) から35.3μm (実験群, n=1, 462) に, IIA線維は55.0 (対照群, n=722) から50.1μm (実験群n=1, 410) に有意に減少したが, IIB線維では有意な変化はみられなかった. 計測した全筋線維数に占める各タイプの筋線維数の割合 (構成比率) は, タイプI線維は対照群と比較して統計的に有意に減少した (対照群: 40.7%, 実験群: 29.3%). 一方, IIA線維は有意な増加を示した (対照群: 17.6%, 実験群: 30.5%). 過去の筋に対する負荷を低下させた四肢筋での研究では, 負荷の低下はSとFRタイプの運動単位に属するタイプIとIIA線維の萎縮を引き起こすだけでなく, ミオシン重鎖の出現型をI→IIa→IId/x→IIbと変化させることが明らかにされている. したがって, 粉末飼料飼育による負荷の低下は, SならびにFRタイプの運動単位の活動量を低下させ, その結果, タイプIとIIA線維に萎縮が生じたと思われる. また, タイプI線維の構成比率の減少と, タイプIIA線維の構成比率の増加の背景には, おそらくミオシン重鎖のI型からIIa型への変換が存在すると思われた.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"1 1","pages":"7-18"},"PeriodicalIF":0.0,"publicationDate":"2002-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88850035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the Susceptibility of Dentin Phosphoproteins to Proteases and Heat Treatment. 牙本质磷蛋白对蛋白酶和热处理敏感性的评价。

Japanese Journal of Oral Biology Pub Date : 2001-12-20 DOI: 10.2330/JORALBIOSCI1965.43.659

Tomokazu Nakajima, R. Fujisawa, Y. Kuboki

{"title":"Evaluation of the Susceptibility of Dentin Phosphoproteins to Proteases and Heat Treatment.","authors":"Tomokazu Nakajima, R. Fujisawa, Y. Kuboki","doi":"10.2330/JORALBIOSCI1965.43.659","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.659","url":null,"abstract":"Phosphophoryn (PP) is a unique phosphoprotein of dentin . Previous investigators have reported that PP degrades during tooth maturation, but its degradation mechanism has not been determined . In this study, we attempted to examine the susceptibility of PP to proteases , using SDS-PAGE with sensitive staining, and we discussed the mechanisms of PP degradation . A human molar, divided into crown and root, was analyzed by SDS-PAGE. The crown fragment contained more lower molecular weight components than the root fragment, indicating that human PP is degraded in vivo during maturation . To examine the mechanisms of the degradation, we investigated the susceptibility of PP to proteases and heat treatment . Rat PP preparations were subjected to matrix metalloproteinase-2 (MMP-2) , trypsin or heat treatment. Major bands of rat PP were observed at 74 and 70kDa. These two bands were clearly observed in the sample treated with MMP-2 or trypsin, but in the heat-treated sample, the proteins corresponding to the major bands were degraded and only diffuse staining was observed . Enzymatic PP degradation was not observed for MMP-2 and trypsin. The non-enzymatic degradation, such as β elimination , as well as enzymatic processes by other MMPs, might contribute to PP degradation. 抄録:象牙質ホスホホリン(PP)は,象牙質特有のタンパク質である。これまでの研究により,歯の成熟に伴いPPが分解を受けることが報告されているが,その分解機構は明らかではない。本研究では,PPのプロテアーゼに対する感受性をゲル電気泳動で高感度染色法を用いて検討し,PPの分解機構について考察した。ヒト第3大臼歯を歯冠と歯根に分割し,ゲル電気泳動で分析したところ,歯冠のほうが歯根より分子量分布が,低分子量側ヘシフトしていた。これは,ヒトPPが,in vivoで分解を受けることを示している。分解機構の解明のため,PP のプロテアーゼ(MMP-2,トリプシン)または,熱処理に対する感受性を検討した。ラットPPの主なバンドは, 74と70kDaにみられた。MMP-2,トリプシンを作用させたサンプルは,主要なバンドに変化はなくPPの分解はみられなかった。熱処理したサンプルでは主要なバンドは形成されず,diffuseな染色性を示し,PPの分解がみられた。本研究では,MMP-2,トリプシンに関して,酵素的分解はみられなかった。PPの分解はほかのMMPs による酵素的分解と同様に,非酵素的分解,β 脱離反応が重要であると思われた。","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"85 1","pages":"659-665"},"PeriodicalIF":0.0,"publicationDate":"2001-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77607365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mapping of a Gene Causing Mouse Hypodontia to Chromosome 13 致小鼠下颌畸形的13号染色体基因定位

Japanese Journal of Oral Biology Pub Date : 2001-12-20 DOI: 10.2330/JORALBIOSCI1965.43.690

Kazuyuki Saito, Y. Asada

引用次数: 0

Comparison of Ca2+-Independent Phospholipase A2 Activity in Apical Plasma Membranes and Secretory Granules from the Rat Parotid Gland 大鼠腮腺顶质膜和分泌颗粒中Ca2+非依赖性磷脂酶A2活性的比较

Japanese Journal of Oral Biology Pub Date : 2001-12-20 DOI: 10.2330/JORALBIOSCI1965.43.666

H. Inokuchi, M. Mizuno-Kamiya, A. Fujita

引用次数: 0