Mapping of a Gene Causing Mouse Hypodontia to Chromosome 13

Abstract

The purpose of this study was to identify the major candidate chromosome and to detect the region that included the candidate gene (s) causing absence of the third molars in EL/sea mice. The candidate chromosomal analysis was performed on genetic crosses using two strains of mice, EL with absence of the third molars, and DDY with a normal complement of teeth. Linkage analysis by interval mapping with microsatellite markers suggested that mouse chromosome 13 was one of the candidate chromosomes and therefore this chromosome was investigated in detail by individual genotyping of F 2 intercross mice with missing third molars. The highest scores were found at D13Mit78 and D13Mit35 markers (xx2=11.8, p< 0.01). Based on these findings, it is suggested that the gene causing absent third molars in EL mice maps close to these microsatellite loci.