Japanese Journal of Oral Biology最新文献_第4页

Expression of Glial Cell Line-Derived Neurotrophic Factor in Myoepithelial Cells of Mouse Tongue Salivary Glands 胶质细胞系来源的神经营养因子在小鼠舌唾液腺肌上皮细胞中的表达

Japanese Journal of Oral Biology Pub Date : 2002-06-20 DOI: 10.2330/JORALBIOSCI1965.44.238

M. Takeda, Nobuhiko Uchida, Yuko Suzuki, N. Obara, Y. Nagai

{"title":"Expression of Glial Cell Line-Derived Neurotrophic Factor in Myoepithelial Cells of Mouse Tongue Salivary Glands","authors":"M. Takeda, Nobuhiko Uchida, Yuko Suzuki, N. Obara, Y. Nagai","doi":"10.2330/JORALBIOSCI1965.44.238","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.238","url":null,"abstract":"ganglion neurons4), and peripheral autonomic neurons5). GDNF mRNA is expressed in various parts of the developing central nervous system and peripheral tissues including the kidneys, teeth and skeletal muscles6). In particular, an abundant expression of GDNF mRNA is indicated in the smooth muscle layers of the intestine during embryogenesis7). Myoepithelial cells are found close to the acini and the intercalated ducts in the salivary glands, occupying the space between the basement membrane and basal plasma membrane of secretory epithelial cells, and are very similar to smooth muscle cells because of the presence of actin and myosin filaments in their cytoplasm and their ability to contract. Here we immunocytochemically found GDNF expression in the cells surrounding the acini and ducts of the lingual salivary glands, which we presumed to be myoepithelial cells. We therefore compared GDNF-positive cells with the cells stained by phalloidin, which labels F-actin. GDNF is shown to interact with a specific cell-surface receptor, GFRα1, and its biological effects are mediated through the interaction of GDNF, GFRα1, and a tyrosine kinase receptor, Ret8) . We therefore additionally examined the expression of GFRα1 and Ret in the lingual salivary glands using an immunocytochemical method.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"38 1","pages":"238-242"},"PeriodicalIF":0.0,"publicationDate":"2002-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77171990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Modulation of Neural Activity in the Lateral Nucleus of the Amygdala by the Propagation of Synchronous Burst Discharges from Parahippocampal Neurons 海马旁神经元同步突发放电的传播对杏仁核外侧核神经活动的调节

Japanese Journal of Oral Biology Pub Date : 2002-06-20 DOI: 10.2330/JORALBIOSCI1965.44.195

M. Funahashi

{"title":"Modulation of Neural Activity in the Lateral Nucleus of the Amygdala by the Propagation of Synchronous Burst Discharges from Parahippocampal Neurons","authors":"M. Funahashi","doi":"10.2330/JORALBIOSCI1965.44.195","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.195","url":null,"abstract":"海馬体と扁桃体は辺縁系を構成する2大領域であり, 大脳皮質や皮質下の諸核と密接な線維連絡をもつとともに,視床下部とも強力な相互連絡をもち,本能行動や情動行動,さらには自律神経調節をも制御している1)。扁桃体緒核のうち外側核は,恐怖条件付けによる回避行動の発現に関与しており,大脳皮質および周辺皮質からの感覚情報を最初に受ける場所としてよく知られている2-7)。恐怖条件付け行動における学習・記憶の形成に関する研究によく用いられてきたのが,ラットの足底の電気刺激を無条件刺激に,一定のトーンによる聴覚刺激を条件刺激にし,トーンによる聴覚刺激を与えてから回避行動発現に要する時間を測定するといった行動実験で, 電気刺激と聴覚刺激を繰り返すとより短い時間で回避行動を行うようになり,やがて回避行動がトーンによる刺激だけでも起こるようになる現象について調べたものである5)。このような行動学的研究によって,視床および大脳皮質連合野の聴覚野からの入力が,扁桃体外側核に収束していることがわかったのをはじめとして,感覚情報の扁桃体外側核への伝達経路が次第に明らかにされていった。一方,記憶形成の主体部分と考えられる海馬体からの入力については,いくつかの報告8-12)があるものの不明な点が多く残されている。すなわち,さまざまな情動刺激を受けることにより生じる感覚情報が,視床や大脳皮質を経由して最終的に扁桃体外側核へ送られる過程において,海馬体からいかなる入力を受け,またそれが感覚情報の修飾・統合にどのように関与するのかを明らかにすることは,感覚情報と記憶とのかかわりを知るうえでもたいへん重要である。図1は,恐怖条件付けにかかわる神経回路をモデルにして,扁桃体外側核への入力経路を模式的に示したものである。このようにたいへん複雑な線維連絡をもつ辺縁系の神経連絡や神経回路網の活動を調べることは容易ではないため,われわれの研究では主に脳スライス標本を用いている。特に側頭葉の水平断スライスを用いることにより,海馬体と扁桃体の両方を同時に同じスライスの上で調べることができる。以下に述べる電気生理学的知見のすべてが,このようなスライス標本から得られたものである。本稿では,われわれの研究により得られた知見をもとに,傍海馬領域および扁桃体外側核の単一ニューロン特性と海馬体から扁桃体への情報伝達様式,さらに傍海馬領域からの同期性入力による扁桃体神経活動の修飾についてまとめ,扁桃体における感覚情報が海馬体からの入力によっていかに修飾・統合されるのかを考察した。","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"67 1","pages":"195-202"},"PeriodicalIF":0.0,"publicationDate":"2002-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88473346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aging of Molars and Jaw Bones in Wild Mice, Mus musculus molossinus 野生小鼠磨牙和颌骨的衰老，molossinus

Japanese Journal of Oral Biology Pub Date : 2002-06-20 DOI: 10.2330/JORALBIOSCI1965.44.203

Y. Takada, E. Sakai, Y. Uematsu, T. Tateishi

引用次数: 1

Apoptosis, Keratin, and Occludin during the Development of Furrows in the Circumvallate Papillae and Ductal Lumina in the Glands of von Ebner. 细胞凋亡、角蛋白和Occludin在von Ebner腺体周围乳头和导管腔沟槽发育过程中的作用。

Japanese Journal of Oral Biology Pub Date : 2002-06-20 DOI: 10.2330/JORALBIOSCI1965.44.225

Y. Nagai, Yuko Suzuki, N. Obara, M. Takeda

{"title":"Apoptosis, Keratin, and Occludin during the Development of Furrows in the Circumvallate Papillae and Ductal Lumina in the Glands of von Ebner.","authors":"Y. Nagai, Yuko Suzuki, N. Obara, M. Takeda","doi":"10.2330/JORALBIOSCI1965.44.225","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.225","url":null,"abstract":"Using mice from embryonic day 18 (E 18) to postnatal day 2 (P 2), we examined apoptosis, keratin and occludin in order to explore the mechanism for the formation of furrows in the circumvallate papillae and ductal lumina in the glands of von Ebner. At E 15, the epithelial cords of the papillae penetratedfrom the dorsal surface into the connective tissue, and at E 18 the epithelial cords of the glands further extended from the base of the papillary cords. Some small spaces appeared in both cords at E 18 and the spaces increased in number, fused, and enlarged to form furrows and ductal lumina by P 2. Apoptotic cells were distributed around and within the spaces in both cords from the examined E 18 to P 2, playing a role in deleting useless cells and sculpting. Cytokeratins 8/18 existed in the central cells of both cords after E 18, including a single cell layer around the spaces. These cells contained reticular keratin filaments. Occludin, localized at tight junctions, appeared along the apical parts of the cells around the spaces in both cords after E 18. Cytokeratins 8/18-and occludin-positive cells around the spaces disappeared from the papilla after completion of the furrow at P 1 or P 2; however, they remained unchanged around the ductal lumina of the glands. This cell layer plays a role in maintaining the spaces and providing elasticity in the process of fusion and enlargement of the spaces during development.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"80 1","pages":"225-237"},"PeriodicalIF":0.0,"publicationDate":"2002-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75617032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Anatomical Studies on the Stereo Structure of the Lingual Papillae and Their Connective Tissue Cores in the Japanese Serow and Bighorn Sheep 日本羊和大角羊舌乳头及结缔组织核立体结构的比较解剖学研究

Japanese Journal of Oral Biology Pub Date : 2002-04-20 DOI: 10.2330/JORALBIOSCI1965.44.127

Takayuki Yamaguchi, Tomoichirou Asami, Kan Kobayashi

{"title":"Comparative Anatomical Studies on the Stereo Structure of the Lingual Papillae and Their Connective Tissue Cores in the Japanese Serow and Bighorn Sheep","authors":"Takayuki Yamaguchi, Tomoichirou Asami, Kan Kobayashi","doi":"10.2330/JORALBIOSCI1965.44.127","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.127","url":null,"abstract":"草食性動物で偶蹄目のニホンカモシカとオオツノヒツジの舌と舌乳頭について, 光顕と走査電顕により観察し, 比較解剖学的に検討した.糸状乳頭の外形に関しては, 両種ともに太い1本の主突起と, 細長い数本の副突起からなる. 糸状乳頭の結合織芯は, ニホンカモシカでは円筒形の基部の周辺から細長い2次芯が馬蹄形に並び, オオツノヒツジでは多数の小突起が馬蹄形に並び, 後方の数本の基部が癒合している. 茸状乳頭の結合織芯は, 両種ともに1次芯が先端の丸まった円柱状で, ニホンカモシカでは側面に縦走するヒダがあり, オオツノヒツジでは側壁や上面は小さく尖った2次芯でおおわれる. 上面には両種共味蕾を入れる小陥凹が数個ある. 舌隆起部には大型円錐乳頭が分布し, 芯の基部は大きく, 表面は多数の小突起 (2次芯) でおおわれる.有郭乳頭の数は, ニホンカモシカでは約20個, オオツノヒツジでは両側に数個ずつある. 中央乳頭部の溝側上皮内に多数の味蕾をもち, 輪状溝の底に漿液腺の導管が多数開口する. 両種ともに葉状乳頭はない. 舌根部表面は平坦で, 上皮下によく発達した粘液腺が分布する.以上から, ニホンカモシカでは舌隆起部の大型円錐乳頭の結合織芯はウシに酷似するが, 糸状乳頭と茸状乳頭の結合織芯はイエウサギのものに似る. オオツノヒツジの糸状乳頭の結合織芯は, 同じ偶蹄目で草食性動物のなかにあって, ニホンカモシカとプロングホーンとの中間に位置する立体構造をもち, この構造はさらにシロイワヤギからシバヤギへと複雑化し, ウシの癒合型へと進化したことが推測される.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"80 1","pages":"127-141"},"PeriodicalIF":0.0,"publicationDate":"2002-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91068698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Changes in Activities of the Jaw and Tongue Muscles Following Severance of the Unilateral Hypoglossal Nerve (Medial Branch) in Awake Cats 清醒猫单侧舌下神经(内侧支)断裂后下颌和舌头肌肉活动的变化

Japanese Journal of Oral Biology Pub Date : 2002-04-20 DOI: 10.2330/JORALBIOSCI1965.44.96

H. Hiraba, Takako Sato, Koko Manabe, M. Hori, Syuuko Imura, Hiroshi Tanaka

引用次数: 3

Historical Change of Dental Carious Lesions from Prehistoric to Modern Times in Japan 日本史前至近代龋病的历史变迁

Japanese Journal of Oral Biology Pub Date : 2002-04-20 DOI: 10.2330/JORALBIOSCI1965.44.87

H. Fujita

{"title":"Historical Change of Dental Carious Lesions from Prehistoric to Modern Times in Japan","authors":"H. Fujita","doi":"10.2330/JORALBIOSCI1965.44.87","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.87","url":null,"abstract":"The aim of this study was to document the historical change of dental caries lesions in the Japanese people. The majority of carious lesions in historical populations from the Jomon to Edo period were located in the neck and/or root of the approximal surface (NRAS). Caries on the occlusal surface (OS) were rare in both the Jomon and Kofun populations, but there was a relatively high rate (17.6-24.7%) in the Kamakura, Muromachi and Edo periods. People in both the Jomon and Kofun periods had severe dental attrition; therefore, due to the disappearance of fissures and pits, caries on the OS did not arise. However, after the Kamakura period, the attrition was moderate, so occlusal caries increased. Caries on the lingual surface (LS) and the neck and/or root of the lingual surface (NRLS) were rare in all Japanese periods. The incidence may be associated with a self-cleaning action with the tongue and the saliva. In the modern population, the most frequent lesion is on the approximal surface (AS), followed by the NRAS and the OS. The rate of coronal caries is higher than that of root caries only in modern times. It seems reasonable to suppose that the former is a modern type of caries, and the latter is ancient. Furthermore, it can be said that from the Jomon to Kofun or from the Kofun to Kamakura periods, and from the Edo to modern times, are turning points for different types of caries in the history of Japan. The type of carious lesion is a good indicator of oral health condition, including dietary habits, subsistence and lifestyle in each period.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"5 1","pages":"87-95"},"PeriodicalIF":0.0,"publicationDate":"2002-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89628351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Biophysical and Pharmacological Properties of Voltage-gated Calcium Channels in Osteoblastic MC3T3-E1 Cells 成骨细胞MC3T3-E1电压门控钙通道的生物物理和药理特性

Japanese Journal of Oral Biology Pub Date : 2002-04-20 DOI: 10.2330/JORALBIOSCI1965.44.120

Mutsuhito Tatamiya, H. Hotokezaka, N. Yoshida, Kazuhide Kobayashi, Toshihide Sato, Y. Okada

{"title":"Biophysical and Pharmacological Properties of Voltage-gated Calcium Channels in Osteoblastic MC3T3-E1 Cells","authors":"Mutsuhito Tatamiya, H. Hotokezaka, N. Yoshida, Kazuhide Kobayashi, Toshihide Sato, Y. Okada","doi":"10.2330/JORALBIOSCI1965.44.120","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.120","url":null,"abstract":"The electrophysiological and pharmacological properties of the voltage-gated Ca2+ channels in MC3T3-E1 cells were analyzed using the perforated whole-cell patch-clamp technique. When the voltage was depolarized by step pulses from a holding potential of -104mV, the cells displayed transient inward currents (-4.25±0.25pA/pF, n=16) in 10mM Ba2+ solution. The activation threshold for the inward Ba2+current was about -60mV and the peak existed between -40 and -20mV. The steady state activation and inactivation properties of the inward Ba2+ current generated a window current in the range of -70 to -40 mV. Gd2+ (0.1mM) inhibited the inward Ba2+ currents by about 60%. Ni2+ (0.1mM, a blocker for T-type and R-type Ca2+ channels at this concentration), nifedipine (5μM, L-type Ca2+ channel blocker), ω-conotoxin GVIA (3μM, N-type Ca2+ channel blocker) and ω-agatoxin TK (200nM, a P/Q-type Ca2+ channel blocker) did not inhibit the currents. Bay K 8644 (0.5μM, a dihydropyridine agonist for L-type Ca2+ channel) also did not affect the Ba2+ currents. The results suggest that Ca2+ channels with novel properties are expressed in MC3T3-E1 cells.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"2012 1","pages":"120-126"},"PeriodicalIF":0.0,"publicationDate":"2002-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86391193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Clonality of Black-pigmented Anaerobes Isolated from Infected Root Canals and Subgingival Plaque in 12 Patients 12例感染根管及龈下菌斑中黑色厌氧菌的克隆分析

Japanese Journal of Oral Biology Pub Date : 2002-04-20 DOI: 10.2330/JORALBIOSCI1965.44.106

M. Okamoto, Reiko Osada, T. Arai, N. Maeda

{"title":"Clonality of Black-pigmented Anaerobes Isolated from Infected Root Canals and Subgingival Plaque in 12 Patients","authors":"M. Okamoto, Reiko Osada, T. Arai, N. Maeda","doi":"10.2330/JORALBIOSCI1965.44.106","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.106","url":null,"abstract":"The purpose of this study was to investigate the clonal types of black-pigmented anaerobes isolated from infected root canals and subgingival plaque. Twelve patients, who had single-rooted teeth with selected chronic apical periodontitis and had not undergone endodontic treatment, were chosen for thisstudy. Microbiological specimens from the root canals were collected with three sizes of H-files, and thosefrom six subgingival sites of the same teeth were collected with paper points. The isolates were identified by the 16S rRNA gene-directed PCR method, and the gene types were determined by the arbitrarily primed PCR (AP-PCR) method. Porphyromonas gingivalis and Prevotella nigrescens were the most frequently isolated species from the root canals (41.7%) and from subgingival plaque samples (50.0%) in 12 patients, respectively. Four patients simultaneously harbored the same species in the root canal and subgingival plaque (three patients harbored P. nigrescens and one patient P. gingivalis). The AP-PCR patterns of isolates from the root canal and subgingival plaque were identical in at least three out of 12 patients. These results support the hypothesis that the pocket is one of the possible sources of root-canal infection.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"5 1","pages":"106-113"},"PeriodicalIF":0.0,"publicationDate":"2002-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74162931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between Physical Properties of a Food Bolus and the Swallowing Threshold during Mastication of Gel Type Food. 凝胶型食物咀嚼过程中食丸的物理性质与吞咽阈值的关系。

Japanese Journal of Oral Biology Pub Date : 2002-04-20 DOI: 10.2330/JORALBIOSCI1965.44.114

K. Shiozawa, K. Kohyama, K. Yanagisawa

{"title":"Relationship between Physical Properties of a Food Bolus and the Swallowing Threshold during Mastication of Gel Type Food.","authors":"K. Shiozawa, K. Kohyama, K. Yanagisawa","doi":"10.2330/JORALBIOSCI1965.44.114","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.114","url":null,"abstract":"To study which physical properties of a food bolus trigger swallowing during mastication of gelatinous food, we measured the texture of a bolus immediately prior to swallowing by texture profileanalysis. Filter paper soaked in 0.2M tartaric acid (acid stimulation) or distilled water (DW stimulation) was placed on the dorsal surface of the tongue of 10 healthy adult participants for 1 minute before they masticated rice cake (RC) or gummy candy (G). The G bolus was significantly (p<0.05) harder immediately prior to swallowing after acid stimulation than after DW stimulatien. On the other hand, hardness, adhesive. ness and cohesiveness of the RC bolus did not differ significantly between the two masticatory conditions (after acid stimulation and DW stimulation). After DW stimulation, the texture of the RC bolus during the middle stage of mastication was compared with that just before swallowing. The RC bolus at the middle stage was significantly harder (p<0.001) and more adhesive (p<0.05) than just before swallowing. These results suggest that the degree of adhesiveness of a bolus might be closely related to the swallowing threshold for gelatinous food such as rice cakes.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"136 1","pages":"114-119"},"PeriodicalIF":0.0,"publicationDate":"2002-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76401961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11