Japanese Journal of Oral Biology最新文献_第3页

Involvement of P/Q-type Voltage-dependent Calcium Channels in the Streptozotocin-induced Hyperalgesia in Mice P/ q型电压依赖性钙通道参与链脲佐菌素诱导的小鼠痛觉过敏

Japanese Journal of Oral Biology Pub Date : 2003-02-20 DOI: 10.2330/JORALBIOSCI1965.45.8

Tadaoki Fukuizumi, T. Ohkubo, K. Kitamura

引用次数: 2

Expression of Hsp47 in Fibroblasts Derived from Fetal and Neonatal Rat Tongues Hsp47在胎鼠和新生鼠舌成纤维细胞中的表达

Japanese Journal of Oral Biology Pub Date : 2002-12-20 DOI: 10.2330/JORALBIOSCI1965.44.541

S. Ohba, T. T. Baba, T. Nemoto, T. Inokuchi

{"title":"Expression of Hsp47 in Fibroblasts Derived from Fetal and Neonatal Rat Tongues","authors":"S. Ohba, T. T. Baba, T. Nemoto, T. Inokuchi","doi":"10.2330/JORALBIOSCI1965.44.541","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.541","url":null,"abstract":"Scar formation, caused by the abnormal expression and accumulation of collagen molecules accompanying the expression of heat shock protein 47 (Hsp47), a collagen-specific molecular chaperone, is a serious problem after surgery in the postnate, whereas in the fetus, the wound heals without scarring. In this study, we compared the expression patterns of Hsp47 and type I and type III collagens induced by TGF-β1 between fetal and neonatal fibroblasts in the primary cultures of rat tongues. In the neonate, high level expressions of both Hsp47 and collagen mRNAs and proteins were observed to be induced by TGF-β1, but not in the fetus. We performed a reporter assay using pLUC 5.5 (III), which carried the enhancer/promoter region of the mouse Hsp47 gene, to compare promoter/reporter activity. In the neonate, high promoter/ reporter activity in fibroblasts treated with TGF-β1 was observed, but was unchanged in the fetus. Thus, the expression of Hsp47 is enhanced by TGF-β1 in the neonate but not in the fetus. This different Hsp47 expression pattern between the fetus and neonate appears to be attributed to the different transcriptional regulation of the gene. Elucidation of the regulatory mechanism of Hsp47 production in developmental processes may provide a therapeutic modality for the scarless healing of postnatal wounds.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"26 1","pages":"541-548"},"PeriodicalIF":0.0,"publicationDate":"2002-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82277710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Simultaneous Determination of Alteration of a Variety of Macrophage Functions Related to Natural Immunity Following Treatment with a DP Receptor Agonist DP受体激动剂治疗后巨噬细胞自然免疫相关功能改变的同时测定

Japanese Journal of Oral Biology Pub Date : 2002-12-20 DOI: 10.2330/JORALBIOSCI1965.44.522

Kiyoshi Daito, Y. Azuma, M. Daito, K. Ohura

引用次数: 2

Morphological Study of the Parotid Duct 腮腺导管的形态学研究

Japanese Journal of Oral Biology Pub Date : 2002-12-20 DOI: 10.2330/JORALBIOSCI1965.44.515

K. Amano, H. Shiga, R. Sukekawa, I. Itoh

引用次数: 1

Japanese Journal of Oral Biology Pub Date : 2002-12-20 DOI: 10.2330/JORALBIOSCI1965.44.530

T. Miyamoto, M. Mizuno, M. Tamura, M. Kawanami

{"title":"Osteoblast-Related Gene Expression of Rat Bone Marrow Cells Induced by Three-dimensional Cell Culture in Type I Collagen Gel","authors":"T. Miyamoto, M. Mizuno, M. Tamura, M. Kawanami","doi":"10.2330/JORALBIOSCI1965.44.530","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.530","url":null,"abstract":"Bone marrow stromal cells contain multipotent stem cells that differentiate into several types of cells under certain conditions in culture. Previously, we reported that a type I collagen matrix induced the osteoblastic differentiation of rat bone marrow stromal cells in culture. In this study, we performed a three dimensional culture within type I collagen gel to elucidate the relationship between the extracellular matrix of the cell culture system and the osteoblastic differentiation of rat bone marrow stromal cells. We compared the osteoblastic gene expressions of the culture cells; (i) culture in conventional culture dish (2D group), (ii) culture in type I collagen-coated culture dish (COAT group), and (iii) culture in three-dimensional type I collagen gel (3D group). In the 3D group of cell culture, the alkaline phosphatase activities of cells were higher than those of the other groups over 3 weeks of culture. Moreover, bone sialoprotein and osteocalcin mRNA expressions were found to be induced by the three-dimensional type I collagen gel culture of rat bone marrow stromal cells. These results suggested that the three dimensional culture of rat bone marrow cells is a crucial factor for inducing osteoblastic differentiation. 抄録:高等動物の骨髄間質細胞のなかには,多分化能を有する間葉系幹細胞が存在し,培養下においても,種々の条件によりさまざまな細胞に分化しうると考えられている。われわれは,これまでにラット骨髄間質細胞をI 型コラーゲンとともに培養することにより,骨芽細胞への分化が誘導されることを報告してきた。本研究では, 〒060-8586 北海道札幌市北区北13条西7丁目宮本哲朗ほか:ラット骨髄間質細胞のコラーゲンゲル内三次元培養 531 細胞の三次元的配置と細胞分化との関連を明らかにする目的で,ラット骨髄間質細胞を通常の培養用ディシュにて単層培養した群(2D群),I型コラーゲンをコートしたディシュにて培養した群(COAT群)ならびにI型コラーゲンゲル内にて三次元培養を行った群(3D群)にてそれぞれ培養し,遺伝子発現を比較検討した。3D群において,ほかの2群と比較して,アルカリホスファターゼ活性が高く,骨シアロプロテインおよびオステオカルシンの遺伝子発現が誘導された。本研究により,ラット骨髄間質細胞をI型コラーゲンゲル内で三次元的に培養することにより,骨芽細胞の分化が誘導されることを明らかにした。","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"46 1","pages":"530-540"},"PeriodicalIF":0.0,"publicationDate":"2002-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89956488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Expression and Nuclear Localization of p65 in Brain and Retina of Streptozotocin-induced Diabetic Rats with Hyperglycemia 链脲佐菌素诱导的糖尿病高血糖大鼠脑和视网膜中p65的表达和核定位

Japanese Journal of Oral Biology Pub Date : 2002-08-20 DOI: 10.2330/JORALBIOSCI1965.44.274

Kiyoshi Daito, Y. Azuma, Pao‐Li Wang, S. Shirasu, Mikio Kato, M. Shinohara, M. Daito, K. Ohura

{"title":"Expression and Nuclear Localization of p65 in Brain and Retina of Streptozotocin-induced Diabetic Rats with Hyperglycemia","authors":"Kiyoshi Daito, Y. Azuma, Pao‐Li Wang, S. Shirasu, Mikio Kato, M. Shinohara, M. Daito, K. Ohura","doi":"10.2330/JORALBIOSCI1965.44.274","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.274","url":null,"abstract":"De novo protein synthesis in eukaryotes is mainly controlled at the level of gene transcription by transcription factors in the nucleus. In this communication, the expression and nuclear localization of the Rel/NF-κB family member p65 in nuclear and cytosolic fractions of the brain and retina from streptozotocin (STZ) -injected diabetic rats were determined in order to evaluate the possibility that the transportation of Rel/NF-κB family members from the cytoplasm to the nucleus could play a role in diabetes. Immunoblotting assays with a specific antibody revealed a much higher expression of immunoreactive p65 in nuclear fractions than in cytosolic fractions of the cerebral cortex, hippocampus, midbrain and cerebellum after diabetes induction by STZ administration. In contrast, STZ administration suppressed the p65 nuclear localization detected in the striatum, hypothalamus, medulla-pons and retina. These results suggest that STZ-inlected diabetic rats show differential alterations in the nuclear localization of p65 protein in the brain and retina.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"8 1","pages":"274-281"},"PeriodicalIF":0.0,"publicationDate":"2002-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78126997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Immunohistochemical Studies on Physiological Root Resorption of Rat Molars 大鼠磨牙生理性牙根吸收的免疫组织化学研究

Japanese Journal of Oral Biology Pub Date : 2002-08-20 DOI: 10.2330/JORALBIOSCI1965.44.282

Yotaro Tadatomo, M. Komatsu, M. Kagayama

引用次数: 2

Cloning and Characterization of the Streptococcus mutans Pyruvate Formate-Lyase Gene (plf) and Pyruvate Formate-Lyase-Activating Enzyme Gene (act) 变形链球菌丙酮酸甲酸裂解酶基因(plf)和丙酮酸甲酸裂解酶激活酶基因(act)的克隆与鉴定

Japanese Journal of Oral Biology Pub Date : 2002-08-20 DOI: 10.2330/JORALBIOSCI1965.44.265

Yasuhito Yamamoto

引用次数: 0

Bone Wound Healing after Maxillary Molar Extraction in Ovariectomized Aged Rats 老年去卵巢大鼠上颌磨牙拔除后骨创面愈合

Japanese Journal of Oral Biology Pub Date : 2002-08-20 DOI: 10.2330/JORALBIOSCI1965.44.255

Miho Shimizu-Ishiura

{"title":"Bone Wound Healing after Maxillary Molar Extraction in Ovariectomized Aged Rats","authors":"Miho Shimizu-Ishiura","doi":"10.2330/JORALBIOSCI1965.44.255","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.255","url":null,"abstract":"わが国における骨粗鬆症患者は増加の一途にあり,厚生労働省の推計では,男性が約100万人,女性では800万人に迫っており,その予備軍を含めると約2 ,000万人と推定されている。骨粗鬆症の原因には閉経のほか,老化やさまざまな内分泌疾患が考えられており,臨床的には骨量の減少による易骨折をきたす疾患を骨粗鬆症と診断している。本症の大半を占める,加齢に伴って起こる骨粗鬆症(退行期骨粗鬆症)の代表的なものとして,「閉経後骨粗鬆症」と「老人性骨粗鬆症」の2つがあげられる。前者は「閉経に伴うエストロゲンの欠乏」が,後者は「老化に伴う活性型ビタミンDの産生低下や骨形成に大切な腸や腎臓などの臓器の機能低下」が原因と考えられている。前者の閉経によるエストロゲン欠乏がヒトや実験動物において著しい骨量の減少を引き起こすことはよく知られた事実であり,骨髄腔の拡大が主たる組織変化と報告されている。骨組織は常に活発な代謝回転により再構築を繰り返し,その構造と骨量は血中のCa2+代謝に直接かかわる骨形成と骨吸収の平衡関係により維持されている1)。特に閉経後のエストロゲン欠乏は,骨芽細胞系細胞からの IL-6の産生を促進し,破骨細胞形成を促進することにより2-4),骨吸収の亢進による持続的な骨量の減少が起こり5),骨強度が低下する骨粗鬆症を発症する危険性が高まる。このように骨の強度はその量と質,すなわち骨量と骨の内部構造に依存し,骨芽細胞と破骨細胞の機能によって調節されている。歯科臨床における骨粗鬆症","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"1 1","pages":"255-264"},"PeriodicalIF":0.0,"publicationDate":"2002-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88810752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Adsorption of Bovine Serum Albumin and Porcine Enamel Proteins onto Carbonatoapatites: Effects of Adsorbent Composition and Magnesium Ion in Solution 碳酸钙磷灰石对牛血清白蛋白和猪搪瓷蛋白的吸附:吸附剂组成和溶液中镁离子的影响

Japanese Journal of Oral Biology Pub Date : 2002-06-20 DOI: 10.2330/JORALBIOSCI1965.44.210

O. Ikenoya, Kaori Sato, Y. Soeno, T. Aoba

{"title":"Adsorption of Bovine Serum Albumin and Porcine Enamel Proteins onto Carbonatoapatites: Effects of Adsorbent Composition and Magnesium Ion in Solution","authors":"O. Ikenoya, Kaori Sato, Y. Soeno, T. Aoba","doi":"10.2330/JORALBIOSCI1965.44.210","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.210","url":null,"abstract":"エナメル質は炭酸含有アパタイトで構成されている. 結晶・基質タンパクの相互作用によるエナメル質石灰化の制御機構を解明する目的で, 吸着質としてアルブミンとブタエナメルタンパク, 吸着材として組成・性状が判明している24種類の炭酸含有アパタイトを使用して, 結晶・タンパクの吸着反応を調べた. 吸着材は炭酸イオンの置換タイプによって2つのグループに大別できる. ひとつは中性溶液中で合成され, 水酸基 (Aタイプ) とリン酸基 (Bタイプ) の両サイトに炭酸イオンが置換した結晶試料であり, ほかのグループはアルカリ領域 (OH-とCO32-との競合) あるいはフッ素イオンの共存下 (F-とCO32-との合) で合成され, リン酸基への置換のみが検知された結晶試料である. 結晶・タンパクの吸着平衡の分析結果では, いずれのタンパク試料についても, 吸着反応は結晶組成 (CO3, F, Mg) と反応溶液のMgイオンの存在によって影響を受けることが判明した. これらの実験結果から, 形成期エナメル結晶の組成変化は結晶・タンパクの相互作用を不断に変動させることにつながり, 溶液相に含まれるMgイオンは, 結晶表面への吸着と結晶格子への置換によって結晶・タンパク相互作用を可変しうることが示唆された.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"28 1","pages":"210-224"},"PeriodicalIF":0.0,"publicationDate":"2002-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84092540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1