Dominika Forszt, Karolina Gerreth, Jakub Kopczyński, Anna Zalewska, Katarzyna Hojan, Renata Marchewka, Marzena Bielas, Mateusz Maciejczyk
{"title":"The screening assessment of pro-inflammatory, anti-inflammatory, Th1, Th2 and Th17 cytokines in saliva of patients with ischemic stroke.","authors":"Dominika Forszt, Karolina Gerreth, Jakub Kopczyński, Anna Zalewska, Katarzyna Hojan, Renata Marchewka, Marzena Bielas, Mateusz Maciejczyk","doi":"10.7150/ijms.110452","DOIUrl":"10.7150/ijms.110452","url":null,"abstract":"<p><p><b>Introduction:</b> Ischemic stroke leads to hypoxia of brain structures, causes inflammation and tissue necrosis. Since the blood-brain barrier is damaged, inflammatory mediators can enter the bloodstream and saliva. This case-control study examines pro-inflammatory, anti-inflammatory, Th1, and Th2 cytokines in the unstimulated saliva of stroke patients and explores the association between these biomarkers and clinical status. <b>Patients and methods:</b> The study group included 22 patients with ischemic stroke, while the control group included 22 healthy individuals that were matched for age, sex, dental, periodontal and oral hygiene status to the study group. Unstimulated saliva was collected from each patient. In each sample, the inflammatory profile was determined using the multiplex ELISA method. Due to the lack of normal distribution, the Mann-Whitney U test was used for comparisons. The research was approved by the Bioethics Committee of the Poznan University of Medical Sciences (59/19, 890/19, 504/21). <b>Results:</b> In the unstimulated saliva of stroke patients, significantly higher levels of pro-inflammatory cytokines (IL-1β (p=0.0003), TNF-α (p≤0.0001), TNF-β; (p≤0.0001)), anti-inflammatory cytokines (IL-1ra (p≤0.0001), TRAIL (p=0.0206)), Th1 cytokines (IFN-<b>γ</b> (p≤0.0001), IL-2Rα (p=0.0021) and IL-12 (p40) (p≤0.0001)) and Th2 cytokines (IL-6 (p=0.0023)) were found compared to healthy participants. Interestingly, Addenbrooke's Cognitive Examination Revised (ACE-R) correlated negatively with TNF-α (p=0.01, r=-0.53), TNF-β (p=0.02, r=-0.60) and IFN-γ (p=0.02, r=-0.50). The Functional Independence Measure (FIM) exhibited a positive association with IL-6 (p=0.003, r=0.62) and BI (p=0.001, r=0.66). The positive correlation was found between Sitting Balance Scale (SBS) and IL-6 (p=0.02, r=0.52). <b>Conclusions:</b> Stroke patients exhibit altered salivary composition characterized by increased secretion of inflammatory mediators. The obtained results do not indicate the dominance of any of the branches of the immune response. The concentration of salivary TNF-α, TNF-β, IFN-<b>γ</b> and IL-12 significantly distinguish patients with ischemic stroke from healthy individuals. Although validation of the results in a larger patient population is necessary, salivary cytokines show potential as diagnostic biomarkers.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 11","pages":"2686-2699"},"PeriodicalIF":3.2,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Autonomic Nervous System in Acupuncture for Gastrointestinal Dysmotility: From Anatomical Insights to Clinical Medicine.","authors":"Na-Na Yang, Xiao-Xia Xie, Wen-Li Yan, Yi-Duo Liu, He-Xuan Wang, Liu-Xin Yang, Cun-Zhi Liu","doi":"10.7150/ijms.107643","DOIUrl":"10.7150/ijms.107643","url":null,"abstract":"<p><p>The expanding knowledge on the involvement of the autonomic nervous system in regulating digestive tract homeostasis is shedding new light on the therapeutic potential of acupuncture. This review aims to evaluate the effectiveness and autonomic nervous system associated mechanisms of acupuncture in gastrointestinal dysmotility in both laboratory and clinical settings, guiding future studies. Mechanistically, studies with animal models and human subjects demonstrated that acupuncture, as a non-invasive somatosensory stimulation, can help improve gastrointestinal dysmotility via directly modulating the activation of autonomic nervous system. Additionally, acupuncture has been found to suppress inflammatory response and alter the secretion of gastrointestinal hormones via different somatic autonomic reflex pathways. Importantly, the therapeutic effects of acupuncture are affected by, and even rely on, the selected acupoints, the frequency and intensity of stimulation, type of gastrointestinal disorders, and the treatment frequency. Meanwhile, clinical studies indicated that acupuncture shows promise in alleviating gastrointestinal dysmotility from the esophagus to the colon, including conditions like gastroparesis, functional dyspepsia, postoperative ileus, irritable bowel syndrome, and chronic functional constipation. We argued against a narrow classification of abdominal and hindlimb acupoints as solely activating sympathetic or vagal nerve to decrease or increase gastrointestinal motility, respectively. Lastly, incorporating new technologies will assist to reveal central autonomic network changes in response to acupuncture stimulation.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 11","pages":"2620-2636"},"PeriodicalIF":3.2,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The interaction network between intestinal flora and cell death in microecosystem of pan-cancers.","authors":"Yuhan Zhang, Cenzhu Wang, Tingyan Ruan, Shuai Liang, Huning Jiang, Xi Wu, Rui Hou, Hanfang Fan, Huiyu Wang, Junli Ding, Junying Xu","doi":"10.7150/ijms.111723","DOIUrl":"10.7150/ijms.111723","url":null,"abstract":"<p><p>The human intestinal floras play an important role in human microecosystem, accounting for more than 1,500 species and consisting of beneficial, harmful and neutral bacteria, which take part in regulating the progression of various malignant tumors. Meanwhile, the cell death is a physiological process maintaining biological development and internal environmental homeostasis, including cuproptosis, ferroptosis, disulfidptosis, immunogenic cell death, necroptosis, anoikis, autophagy, pyroptosis and so on. A variety of cell deaths and their related genes have been reported to regulate many malignant tumors. In recent years, there has been increasing interest in understanding the interaction network between intestinal flora and cell death in microecosystem of various malignant tumors. However, this interaction network is still not fully understood and requires further investigation. Therefore, the aim of this study is to explore the potential mechanism network between intestinal flora and cell death in pan-cancers, with the hope that this research could bring a novel insight for the prevention and treatment of tumors.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 11","pages":"2596-2608"},"PeriodicalIF":3.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163404/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Deciphering the Relationship Between Circulating Metabolites and Osteoarthritis: A Comprehensive Genetic Correlation and Mendelian Randomization Studies.","authors":"Guang Yang, Wenqing Xie, Hengzhen Li, Wenhao Lu, Ying Liang, Yusheng Li, Yaping Wang, Wenfeng Xiao","doi":"10.7150/ijms.111848","DOIUrl":"10.7150/ijms.111848","url":null,"abstract":"<p><p>The causal impact of blood metabolites on OA has yet to be definitively established, further studies are needed to explore the specific roles of metabolites in OA. This is a genetic correlation and two-sample bidirectional mendelian randomization study. GWAS summary data of metabolites and OA were extracted from large-scale GWAS study based on Europeans and Asians. LDSC was conducted to estimate the genetic correlations between 233 circulating metabolites and 11 OA phenotypes, MR was then performed to explore the casual association. 41.20% of the metabolic traits showed genetic correlation with All OA, 15.88% with Knee/Hip OA, 51.50% with Knee OA, and 52.79% with Spine OA. No significant genetic correlations were detected between the metabolic traits and other OA phenotypes. Lactate levels was associated with increased odds of All OA (OR: 1.1558, P<0.001), Hip OA (OR: 1.1446, P=0.004), Knee/Hip OA (OR: 1.1820, P<0.001), Knee OA (OR: 1.1375, P=0.001), Spine OA (OR: 1.3179, P<0.001), THR (OR: 1.5290, P<0.001), and TJR (OR: 1.2827, P<0.001), except for Thumb OA (OR: 0.9429, P<0.001). Ratio of conjugated linoleic acid to total fatty acids was associated 6 OA phenotypes: Hip OA (OR: 0.9522, P=0.035), Knee/Hip OA (OR: 1.0890, P<0.001), Knee OA (OR: 1.1429, P<0.001), THR (OR: 1.3800, P<0.001), TJR (OR: 1.3102, P<0.001), and TKR (OR: 1.2555, P<0.001). Glycerol levels exhibited significant MR associations with four OA phenotypes: Finger OA (OR: 0.6669, P<0.001), Hand OA (OR: 0.8682, P=0.011), Hip OA (OR: 0.9395, P<0.001), and Knee OA (OR: 1.1409, P=0.036). This study underscores genetic and causal connections between specific metabolites and OA. These findings could inform future therapeutic metabolic pathways involved in OA.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 11","pages":"2609-2619"},"PeriodicalIF":3.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The combination of PF-429242 and chloroquine triggers pH-dependent cell death in hepatocellular carcinoma cells.","authors":"Jiunn-Chang Lin, Tun-Sung Huang, Yan-Bin Chen, Pao-Shu Wu, Tsang-Pai Liu, Pei-Ming Yang","doi":"10.7150/ijms.109069","DOIUrl":"10.7150/ijms.109069","url":null,"abstract":"<p><p>Hepatocellular carcinoma (HCC) remains a significant health challenge due to its resistance to conventional treatments and high recurrence rates. Developing novel therapeutic strategies is critical for improving outcomes for HCC patients. In this study, we identified the synergistic anticancer activity of the combination of PF-429242 and chloroquine against HCC cells. Combined treatment exhibited significant cytotoxicity against HCC cell lines, which was not observed with other therapeutic drugs. Notably, this synergistic effect was not mediated through apoptosis or autophagy. Further investigation revealed that the combination induced pH-dependent cell death, distinct from the previously described alkaliptosis. Unlike alkaliptosis, this cell death mechanism did not involve intracellular alkalinization or the IKKβ/NF-κB/CA9 signaling pathway. We also found that the ATP6V0D1/STAT3 axis, implicated in alkaliptosis, was not crucial for PF-429242/chloroquine-induced cell death. Additionally, site-1 protease inhibition by PF-429242 was not responsible for the observed synergistic effect. While the exact mechanism remains unclear, combined treatment induced a necrosis-like morphology and membrane rupture, which could be prevented by acidifying the culture medium. This research highlighted a novel pH-dependent cell death mechanism in HCC cells and suggests potential therapeutic implications for combining PF-429242 and chloroquine in cancer treatment.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 11","pages":"2583-2593"},"PeriodicalIF":3.2,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Global, regional, and national burden of gastritis and duodenitis from 1990 to 2021 with projections to 2050: a systematic analysis of the Global Burden of Disease Study 2021.","authors":"Lufei Wang, Wei Jiang, Hui Li","doi":"10.7150/ijms.109762","DOIUrl":"10.7150/ijms.109762","url":null,"abstract":"<p><p><b>Background:</b> Gastritis and duodenitis are highly prevalent upper gastrointestinal inflammatory conditions. We estimate the most up-to-date global, regional, and national burden in incidence, prevalence, mortality, and disability-adjusted life-years (DALYs) for gastritis and duodenitis from 1990 to 2021 with projections to 2050. <b>Methods:</b> Population-based data in this study was retrieved from the Global Burden of Diseases, Injuries, and Risk Factors Study 2021 (GBD 2021). We evaluated the temporal trends of age-standardized rates of gastritis and duodenitis prevalence (ASPR), incidence (ASIR), mortality (ASMR), and DALYs (ASDR) across 204 countries and territories, as well as estimated annual percentage changes (EAPC) from 1990 to 2021. Analyses were stratified by sex, age subgroup, socio-demographic index (SDI) at the global, regional, and national level. A Bayesian age-period-cohort model was employed to project ASPR and ASDR of gastritis and duodenitis by sex and age up to 2050. <b>Results:</b> In 2021, 27.20 million (95% UI: 21.85-33.65) individuals globally had gastritis and duodenitis, with an ASIR of 323.24 (95% UI: 261.35-398.64) per 100,000. Globally, the prevalent cases of gastritis and duodenitis in 2021 was higher in females than in males and increased with age, peaking at the fifth decade of life. The number of prevalent cases of gastritis and duodenitis is projected to reach approximately 51.24 million by 2050. In 2021, gastritis and duodenitis caused 2.81 (95% UI: 2.17-3.61) million DALYs worldwide, with an ASDR of 35.64 (95% UI: 27.56-45.77) per 100,000, while the ASMR was 0.54 (95% UI: 0.47-0.62) per 100,000. Despite global decreases in ASPR and ASIR, increasing trends were observed in the low and low-middle SDI regions from 1990 to 2021. The low SDI regions exhibited the highest ASDR of 63.44 (95% UI: 44.08-87.16) per 100,000. Among 21 GBD regions, East Asia exhibited the highest ASPR and ASIR in 2021. The ASPR of gastritis and duodenitis in 2050 is forecast to be 402.14 per 100,000, with an ASDR of approximately 22.09 per 100,000. <b>Conclusion:</b> Despite global reductions in ASPR, ASIR, ASMR, and ASDR between 1990 and 2021, the global burden of gastritis and duodenitis exhibits regional disparities, closely linked to SDI levels. The highest burden of gastritis and duodenitis was observed in East Asia and Sub-Saharan Africa. More potent measures are urgently needed in low SDI regions to forestall the increase of Gastritis and duodenitis burden.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 11","pages":"2570-2582"},"PeriodicalIF":3.2,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Doxycycline Enhances Anticancer Activity of Zoledronic Acid via Inducing ROS and Autophagy in Osteosarcoma Cell Lines.","authors":"Yi-An Li, Hsuan-Ying Chen, Chien-Sheng Hsu, Shun-Cheng Tseng, Eric Hwang, Cheng-Pu Hsieh, Shih-Chieh Hung, Yi-Fu Huang","doi":"10.7150/ijms.108086","DOIUrl":"10.7150/ijms.108086","url":null,"abstract":"<p><p>Zoledronic acid (ZOL) is an inhibitor of osteoclast-mediated bone resorption. It is used to treat osteoporosis and skeletal complications in patients with tumor-induced osteolysis. ZOL is also demonstrated to possess anti-cancer activity in several tumors via apoptosis induction. Doxycycline is well-known antibiotic used in treatment of infections caused by bacteria and certain parasites. In this study, we evaluated the possibility if doxycycline could be used as an effective adjuvant to ZOL against osteosarcoma cells. The data showed that co-treatment with doxycycline at non-toxic dose could significantly increase the anti-viability effect of ZOL in osteosarcoma HOS and MG-63 cells in MTT assay and colony formation assay, and largely increased the levels of apoptotic markers, cleaved caspase 3 and PARP, in ZOL-treated cells. Furthermore, as co-treatment with doxycycline, the levels of ROS and autophagy were enhanced in ZOL-treated cells. Administration of <i>N</i>-acetyl-L-cysteine, a reactive oxygen species (ROS) inhibitor, or autophagy inhibitor chloroquine both reduced anti-growth effect of this combined treatment, indicating that the increased ROS and autophagy should be involved in anti-viability effect of combined treatment with ZOL and doxycycline. Taken together, our findings suggested that combined treatment with ZOL and doxycycline may serve as a potential strategy for treating osteosarcoma.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 11","pages":"2560-2569"},"PeriodicalIF":3.2,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Unraveling the Role of N6-Methylation Modification: From Bone Biology to Osteoporosis.","authors":"Junyi Liu, Xiang Chen, Xijie Yu","doi":"10.7150/ijms.108763","DOIUrl":"10.7150/ijms.108763","url":null,"abstract":"<p><p>N6-methyladenosine (m6A) is the most abundant and reversible epitranscriptomic modification in eukaryotes, playing a pivotal role in regulating various RNA metabolic processes, including splicing, nuclear export, translation and degradation. Emerging evidence indicates that m6A modification is indispensable in biological processes of bone cells such as proliferation, differentiation and apoptosis. Given its pivotal influence on osteoblastogenesis and osteoclastogenesis, m6A modification, particularly via METTL3, has attracted considerable attention in osteoporosis (OP). In this review, we probe the function of m6A modification in intramembranous and endochondral ossification. Furthermore, we summarize the regulatory role of m6A modification in various biological processes in osteoblasts, osteoclasts and osteocytes, focusing on its potential signaling pathways in osteoblast and osteoclast differentiation. Specifically, m6A modulates osteoblast differentiation predominantly through signaling pathways such as Wnt/β-catenin, PI3K/AKT, and BMP/Smad. Concurrently, it regulates osteoclast differentiation and maturation via the RANKL/RANK pathway and its downstream signaling mechanisms. We also discuss recent discoveries that m6A modification regulates OP and further explore its potential clinical value in diagnosing and treating OP. Collectively, m6A modification serves as a crucial regulatory factor in bone metabolism, and a comprehensive understanding of the molecular mechanisms of m6A modification in bone biology is expected to provide new targets for treating OP.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 11","pages":"2545-2559"},"PeriodicalIF":3.2,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Guanxiao Chen, Ting Zhou, Xiaoyu Shen, Wan Shu, Shuyang Yu, Kejun Dong, Piotr Laudański, Klaudia Gutowska, Shuangshuang Cheng, Hongbo Wang
{"title":"Identification of an Anoikis-associated LncRNA Signature to Predict the Clinical Prognosis and Immune Function of Patients with Endometrial Cancer.","authors":"Guanxiao Chen, Ting Zhou, Xiaoyu Shen, Wan Shu, Shuyang Yu, Kejun Dong, Piotr Laudański, Klaudia Gutowska, Shuangshuang Cheng, Hongbo Wang","doi":"10.7150/ijms.107243","DOIUrl":"10.7150/ijms.107243","url":null,"abstract":"<p><p><b>Background:</b> Endometrial cancer is a highly heterogeneous malignancy in women with high mortality, and patients diagnosed with advanced endometrial cancer have a poor prognosis. Anoikis is a form of programmed cell death that is important for cancer development and metastasis. Long non-coding RNAs (lncRNAs) are considered critical regulators of gene expression and key players in cancer biology; however, the effects of anoikis-associated lncRNAs on the prognosis and treatment of patients with endometrial cancer remain unclear. <b>Methods:</b> Using transcriptome sequencing data and clinical information from The Cancer Genome Atlas database, we developed a novel prognostic signature for endometrial cancer based on anoikis-related lncRNAs by combining multivariate regression analysis and least absolute shrinkage and selection operator regression. The signature was validated by receiver operating characteristic (ROC) curve and Kaplan-Meier analyses. After analyzing the relationships between the seven lncRNAs in the signature and tumor progression through gene set enrichment analysis (GSEA), we further explored the differences in immune function and drug sensitivity. Additionally, to investigate the functions of these lncRNAs in the occurrence and development of endometrial cancer, we selected <i>CFAP58-DT</i> to conduct a series of <i>in vitro</i> and <i>in vivo</i> experiments to verify its partial functions. <b>Results:</b> Seven anoikis-associated lncRNAs (<i>CFAP58-DT, AC004585.1, AC103563.9, AL121895.2, AC004596.1, AC010761.4,</i> and <i>AC087564.1</i>) with prognostic value were identified for signature construction. The analysis showed excellent predictive accuracy of the signature (the largest area under the ROC curve = 0.757). GSEA indicated that these lncRNAs may regulate diverse cellular processes, including intercellular interactions, cell proliferation, differentiation, apoptosis, angiogenesis, glucose and fatty acid metabolism, immune responses, and inflammatory regulation. Furthermore, immune analysis revealed a high likelihood of immune evasion and poor immunotherapy efficacy in high-risk patients. However, there were distinct differences in the immune checkpoints and anticancer drug sensitivity between the two patient groups, which may aid in guiding treatment. Finally, our experiments showed that silencing <i>CFAP58-DT</i> significantly affected cell proliferation, promoted apoptosis, and reduced tumor malignancy. <b>Conclusion:</b> Our study highlights the significance of anoikis-associated lncRNAs in endometrial cancer progression and their potential as prognostic markers and therapeutic targets. The signature constructed using these lncRNAs may offer new avenues for endometrial cancer treatment and immunotherapy. The function of <i>CFAP58-DT</i> has been validated <i>in vitro</i> and <i>in vivo</i>, consistent with our previous analysis; however, further research into its upstream and downstream mechanisms is warrante","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 10","pages":"2502-2517"},"PeriodicalIF":3.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12080581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}