International Journal of Medical Sciences最新文献

{"title":"Trends in Blood Pressure Control in US Adult CKD Patients from 1999 to 2018.","authors":"Anbang Sun, Lin Zhu, Yanmin Xia, Yao Zheng, Bicheng Hu, Fanghua Li, Yanhong Liao","doi":"10.7150/ijms.103107","DOIUrl":"10.7150/ijms.103107","url":null,"abstract":"<p><p><b>Background:</b> Blood pressure (BP) control can slow down the progression of chronic kidney disease (CKD) and protect against cardiovascular diseases, significantly improving patient survival. Herein, we analyzed the changes in BP control in adult CKD patients with hypertension in the United States from 1999-2000 to 2017-2018. <b>Methods:</b> National Health and Nutrition Examination Survey (NHANES) data from 1999-2000 to 2017-2018 were analyzed, including 5,510 adult CKD patients with BP above 140/90 mmHg or those under an antihypertensive regimen. <b>Results:</b> The proportion of adult CKD patients with uncontrolled BP decreased from 72.9% in 1999-2000 to 46.6% in 2013-2014, then increased to 56.9% in 2017-2018. Although adult CKD patients with albumin-creatinine rate (ACR) 30-299 mg/g or ACR ≥300 mg/g were more likely to take antihypertensive medication than those with ACR <30 mg/g (PR: 2.76, 95% CI: 1.63-4.79 and PR: 4.59, 95% CI: 2.37-9.51), they were more likely to have uncontrolled BP than those with ACR <30 mg/g ((multivariable-adjusted prevalence ratio (PR): 2.25, 95% CI: 1.39-3.75 and PR: 3.14, 95% CI: 1.71-6.07). Adult CKD patients (eGFR ≥60 mL/min/1.73m²) being aware of their high BP diagnosis were less likely to take antihypertensive medication than those with eGFR 30-59 mL/min/1.73m² (PR: 0.27, 95% CI: 0.09-0.65). <b>Conclusions:</b> These results show that BP control should be reinforced in adult CKD patients, particularly in those with ACR ≥300 mg/g, while patients with eGFR ≥60 mL/min/1.73m² should enhance awareness of taking antihypertensive medication.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 3","pages":"696-707"},"PeriodicalIF":3.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences of the Chest Images Between Coronavirus Disease 2019 (COVID-19) Patients and Influenza Patients: A Systematic Review and Meta-analysis.","authors":"Yingying Han, Zhijia Wang, Xingzhao Li, Zhuan Zhong","doi":"10.7150/ijms.98194","DOIUrl":"10.7150/ijms.98194","url":null,"abstract":"<p><p><b>Background:</b> Coronavirus disease 2019 (COVID-19) and influenza are two infectious diseases that can pose a great threat to human health. We aimed to compare the differences in chest images between patients with COVID-19 and influenza to deepen the understanding of these two diseases. <b>Methods:</b> We searched PubMed, Embase and Web of Science for articles published before December 25, 2023, and performed a meta-analysis using Stata 14.0 with a random-effects model. The study was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. <b>Results:</b> Twenty-six articles with 2,159 COVID-19 patients and 1,568 influenza patients were included in the meta-analysis. By comparing chest computed tomography (CT) and chest X-ray, we found that COVID-19 patients had more peripheral lung lesions (OR=3.66, 95% CI: 1.84-7.31). Although COVID-19 patients had more bilateral lung involvement (OR=1.74, 95% CI: 0.90-3.38) and less unilateral lung involvement (OR=0.67, 95% CI: 0.44-1.02), these two results were not statistically significant. Patients with COVID-19 showed more ground-glass opacities (OR=2.83, 95% CI: 1.85-4.32), reverse halo signs (OR=3.47, 95% CI: 2.37-5.08), interlobular septal thickening (OR=2.16, 95% CI: 1.55-3.01), vascular enlargement (OR=5.00, 95% CI: 1.80-13.85) and crazy-paving patterns (OR=2.63, 95% CI: 1.57-4.41) on chest images than patients with influenza. We also found that compared with influenza patients, pleural effusion was rare in COVID-19 patients (OR=0.15, 95% CI: 0.07-0.31). <b>Conclusions:</b> There are some differences in the manifestations and distributions of lesions between patients with COVID-19 and influenza on chest images, which is helpful to distinguish these two infectious diseases.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 3","pages":"641-650"},"PeriodicalIF":3.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunological Insights into the Causal Link Between Arthritis, Osteoarthritis, and Frailty: An Integrated Analytical Study.","authors":"Shuyang Wen, Yuxin Cai, Qi Zhang, Baizhi Qiu, Yuting Zeng, Shuqi Zheng, Zhishan Ling, Yupeng Xiao, Pengcheng Lu, Peng Zheng, Na Chen, Guozhi Huang, Qing Zeng, Jihua Zou","doi":"10.7150/ijms.104476","DOIUrl":"10.7150/ijms.104476","url":null,"abstract":"<p><p><b>Background:</b> Previous observational studies have observed associations between rheumatoid arthritis (RA), knee osteoarthritis (KOA), hip osteoarthritis (HOA), and frailty, but the causal relationships remain unestablished. <b>Objective:</b> This study aimed to evaluate the causal relationships between RA, KOA, HOA, KneeHipOA, and frailty using Mendelian randomization (MR) and bioinformatics analysis. <b>Methods:</b> We performed two-sample MR to test for causality between RA, KOA, HOA, KneeHipOA, and frailty. Subsequently, we combined our results in a meta-analysis and conducted multiple sensitivity analyses (MR-Egger, weighted median, constrained maximum likelihood and model averaging (cML-MA), and Bayesian weighted MR (BWMR)). We further explored the role of circulating immune cells and the effects of RA and OA-related gene expression on frailty. <b>Results:</b> Genetically determined RA, KOA, HOA, and KneeHipOA were correlated with a higher risk of frailty. The results of multivariate MR analyses were consistent with those of two-sample MR. Gene Ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes analysis indicated that RA and OA-related genes were primarily enriched in various immune responses. Our findings suggested that increases in monocyte cell AC, eosinophil cell AC, and neutrophil cell AC were associated with a higher risk of frailty. <b>Conclusion:</b> This research provides evidence supporting the associations between RA, KOA, HOA, KneeHipOA, and frailty. It also highlights the significant role of circulating immune cells in the development of frailty, indicating the importance of frailty management from an immunological perspective.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 3","pages":"616-629"},"PeriodicalIF":3.2,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of bone-protecting effects of palm carotene mixture in two- and three-dimensional osteoblast/osteoclast co-culture systems.","authors":"Michelle Min-Fang Yee, Kok-Yong Chin, Soelaiman Ima-Nirwana, Ekram Alias, Kien Hui Chua, Sok Kuan Wong","doi":"10.7150/ijms.103445","DOIUrl":"10.7150/ijms.103445","url":null,"abstract":"<p><p><b>Background:</b> Carotene exists naturally in a complex mixture consisting of alpha (α), beta (β), and gamma (γ)-isoforms. Previous studies investigated the effects of individual carotene isomers on bone rather than their actions in a mixture. <b>Purpose:</b> This study explored the bone-protective properties of palm carotene mixture using both two- and three-dimensional co-culture systems. <b>Study design:</b> The viability of human foetal osteoblasts (hFOB 1.19), viability of human monocytic cell line (THP-1), osteoblast differentiation, osteoclast maturation, bone quality and strength were assessed in two- and three-dimensional co-culture system after treatment of palm carotene mixture. <b>Methods:</b> The viability of hFOB 1.19 and THP-1 was determined on day 1, 3, and 6 following treatment of palm carotene mixture. The osteoblast-osteoclast co-culture (ratio of hFOB 1.19 to THP-1 = 2:1) was treated with palm carotene mixture as well as subjected to alkaline phosphatase (ALP) and tartrate resistant acid phosphatase (TRAP) staining on day 21 to assess the osteoblast proliferation and osteoclast maturation. Dual-energy X-ray absorptiometry, micro-computed tomography, universal testing machine, and bone histomorphometry were used to assess the bone parameters of scaffolds co-cultured with osteoblasts and osteoclasts. <b>Results:</b> Palm carotene mixture (3.13 - 50 μg/mL) increased osteoblast viability. Monocyte viability decreased in lower concentration (3.13 - 12.5 μg/mL) but increased in higher concentration (25 - 50 μg/mL) of palm carotene mixture. Treatment with palm carotene mixture (12.5 µg/mL) demonstrated earlier peak for the ALP-positive area on day 14 but decreased total number of TRAP-positive multinucleated cells on day 21. Palm carotene mixture also increased bone volume and osteoblast number in the three-dimensional co-culture system. <b>Conclusion:</b> Palm carotene mixture potentially exhibits beneficial effects on bone by accelerating osteoblast proliferation and suppressing osteoclast maturation. The findings of current study serve as the basis for the further validation through animal experiments and human trials.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 3","pages":"585-603"},"PeriodicalIF":3.2,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783079/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NACC1 accelerates the progression of AML by regulating the ADAM9/PI3K/AKT axis.","authors":"Ying Zhang, Liang Zhong, Peng Wan, Yi Zhao, Meng Wang, Hongyan Zhang, Yang Liao, Ying Deng, Beizhong Liu","doi":"10.7150/ijms.102266","DOIUrl":"10.7150/ijms.102266","url":null,"abstract":"<p><p>Nucleus accumbens-associated protein 1 (NACC1) regulates various types of biological processes. It is a transcription factor associated with cancer. NACC1 is overexpressed in many human malignancies and can regulate the progression, metastasis, and drug resistance of cancer cells. However, its precise role in acute myeloid leukemia (AML) remains unknown. This study aimed to unravel the basic mechanism of NACC1 in AML. Our findings demonstrated that NACC1 is immensely expressed in AML cells. Lentiviral vector-mediated knockdown of NACC1 inhibited the PI3K/AKT signaling pathway. Simultaneously, NACC1 knockdown promoted apoptosis, suppressed the proliferative capacity of AML cells, and resulted in cell cycle arrest during the G0/G1 phase. Additionally, A disintegrin and metalloproteinase 9 (ADAM9) was markedly expressed in AML cells. NACC1 regulated ADAM9 expression. ADAM9 expression was also downregulated after NACC1 knockdown. Concurrently, ADAM9 knockdown affected the activity of AML cells by decelerating the growth rate, promoting apoptosis, and blocking cell cycle progression. In addition, the AKT activator SC79 restored the inhibited cell proliferation after NACC1 knockdown and ADAM9 knockdown. In conclusion, our study suggested that the NACC1/ADAM9/PI3K/AKT axis is crucial for sustaining the survival of AML cells, indicating that NACC1 may be a viable target for treating AML.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 3","pages":"630-640"},"PeriodicalIF":3.2,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycosphingolipids-Dependent Phospholipid Metabolism Enhances Cancer Initiation and Progression through SMPD1/GLTP/B3GALT4/ST8SIA6 Signaling Axis: A Novel Therapeutic Target.","authors":"Liangpan Shi, Nanqi Mao, Zhihua Zheng, Jiangrui Liu, Hao Zhou, Jianbin Hou, Yibin Su","doi":"10.7150/ijms.103834","DOIUrl":"10.7150/ijms.103834","url":null,"abstract":"<p><p>Colorectal cancer (CRC) is a prevalent malignancy with high morbidity and mortality rates globally. Advances in single-cell sequencing technology have enabled comprehensive analyses of tumor cells at single-cell resolution, providing valuable insights into the molecular mechanisms underlying CRC initiation and progression. In this study, we integrated single-cell sequencing data with the TCGA database to identify key molecular pathways involved in CRC pathogenesis. Our analysis revealed that dysregulation of phospholipid metabolism, particularly sphingolipid metabolism, plays a crucial role in CRC development. Specifically, we observed aberrant expression of genes involved in sphingolipid biosynthesis and degradation, as well as altered levels of various sphingolipid metabolites in CRC cells. Furthermore, we identified several potential therapeutic targets, including <i>SMPD1</i>, <i>GLTP</i>, <i>B3GALT4</i>, and <i>ST8SIA6</i>, within the sphingolipid metabolism pathway that could be exploited for the development of novel CRC treatments. Overall, our findings provide novel insights into the molecular mechanisms underlying CRC and highlight the importance of targeting phospholipid metabolism, specifically sphingolipid metabolism, as a potential therapeutic strategy for CRC.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 3","pages":"604-615"},"PeriodicalIF":3.2,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the role of vitamin D in normal organs and those affected by COVID-19.","authors":"Rajendran Peramaiyan, Josephine Anthony, Sureka Varalakshmi, Ashok Kumar Sekar, Enas M Ali, Al Hashedi Sallah A, Basem M Abdallah","doi":"10.7150/ijms.103260","DOIUrl":"10.7150/ijms.103260","url":null,"abstract":"<p><p>The outbreak of COVID-19 has opened up new avenues for exploring the importance of vitamin D in immunity, in addition to its role in calcium absorption. Recently, vitamin D supplementation has been found to enhance T regulatory lymphocytes, which are reduced in individuals with COVID-19. Increased risk of pneumonia and increases in inflammatory cytokines have been reported to be major threats associated with vitamin-D deficiency. Although vaccination reduces the threat of COVID-19 to a certain extent, herd immunity is the long-term solution to overcoming such diseases. Co-administration of vitamin D with certain inactivated vaccines has been reported to enhance the systemic immune response through stimulation of the production of antigen-specific mucosal immunity. COVID-19 was found to induce multiple organ damage, and vitamin D has a beneficial role in various organs, such as the intestines, pancreas, prostate, kidneys, liver, heart, brain, and immune cells. The consequences that occur after COVID-19 infection known as long COVID-19 are also a concern as they accumulate and target multiple organs, leading to immune dysregulation. The present review covers the overall role and impact of vitamin D and its deficiency for various organs in normal conditions and after COVID-19 infection, which is still a serious issue.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 2","pages":"240-251"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11704692/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142948313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering Epigenetic and Post-Translational Modifications in Ferroptosis: A Scientometric and Visualization Study.","authors":"Siyang Cao, Yihao Wei, Yaohang Yue, Deli Wang, Ao Xiong, Hui Zeng","doi":"10.7150/ijms.104222","DOIUrl":"10.7150/ijms.104222","url":null,"abstract":"<p><p><b><i>Background:</i></b> Recent research emphasizes the significant regulatory functions of epigenetic alterations and post-translational modifications (PTMs) in the ferroptosis process. Despite the existing volume of literature, there is a remarkable shortage of comprehensive analyses that systematically trace the evolution of research, map key investigative routes, evaluate the current situation of the field, determine central themes, and predict future directions. This study intends to offer a comprehensive summary of the progress achieved during the past 12 years in comprehending how epigenetic modifications and PTMs regulate ferroptosis. <b><i>Methods:</i></b> The dataset originated from the Web of Science, covering the period from January 1, 2012, to May 21, 2024. By employing advanced analytical tools, we carried out an extensive scientometric assessment in combination with detailed visual data analysis. <b><i>Results:</i></b> The results emphasize the crucial role of China, which contributes 69.59% of the global research output, thereby demonstrating its significant influence on the research trajectory in this domain. Remarkable productivity is manifested at institutions such as Central South University, Shanghai Jiao Tong University, and Zhejiang University. Liu Shuang and Tang Daolin stand out as the most productive authors in this field. The journal <i>Cell Death & Disease</i> leads in terms of publication volume, having published the greatest number of articles related to this area. This study identified hepatocellular carcinoma, mitochondrial diseases, and iron overload as the most prominent diseases explored in this research domain. <b><i>Conclusion:</i></b> This meticulous scientometric assessment is beneficial to both experienced researchers and newcomers by providing essential information and facilitating the derivation of innovative concepts in this field.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 3","pages":"508-527"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Renal dysfunction in people with hidradenitis suppurativa: a multi-center, propensity-score-matched cohort study.","authors":"Shuo-Yan Gau, Chi-Ya Yang, Yun-Feng Li, Chien-Ying Lee, Yu-Jung Su, Hui-Chin Chang, Meng-Che Wu","doi":"10.7150/ijms.102434","DOIUrl":"10.7150/ijms.102434","url":null,"abstract":"<p><p><b>Background:</b> Recent studies suggest a potential link between HS and renal dysfunction. Our objective is to assess the correlation between hidradenitis suppurativa (HS) and renal consequences, specifically focusing on acute kidney injury (AKI), chronic kidney disease (CKD), and end-stage renal disease (ESRD). <b>Methods:</b> This study was performed based on retrospective cohort design. Electronic medical records of participants were retrieved from the US collaborative network in the TriNetX research network. Information from 46,561 individuals with HS was examined alongside an equivalent number of matched controls. Propensity matching was performed for matching confounders. The study spanned from January 1, 2005, to December 31, 2017. Primary outcomes were set as renal dysfunction, including AKI, CKD, and ESRD. <b>Results:</b> Over the 1-year follow-up, people with HS presented a 1.84-fold higher risk of AKI (95% CI, 1.34-2.53) and a 1.37-fold higher risk of CKD (95% CI, 1.02-1.85) than non-HS individuals. Elevated risks persisted over the longer follow-up periods for AKI at 1.51-fold (95% CI, 1.28-1.77) for 3-years-follow-up and 1.47-fold (95% CI, 1.30-1.65) for 5-years-follow-up, respectively. Stratification by sex revealed higher risks in males, and comparison with psoriasis patients indicated increased AKI and CKD risks in HS patients. <b>Conclusion:</b> This study highlights a significant association between HS and renal dysfunction, emphasizing the need for further exploration of shared pathophysiological mechanisms. The findings could offer potential insights into HS-related comorbidities.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 3","pages":"558-564"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PUF60 Promotes Chemoresistance Through Drug Efflux and Reducing Apoptosis in Gastric Cancer.","authors":"Qianhui Liu, Yingqiu Song, Jing Su, Shangbin Yang, Qinghai Lian, Tiantian Wang, Hongbo Wei, Jiafeng Fang","doi":"10.7150/ijms.102976","DOIUrl":"https://doi.org/10.7150/ijms.102976","url":null,"abstract":"<p><p><b>Background:</b> Chemotherapy resistance is a great challenge in the treatment of gastric cancer (GC), so it is urgent to explore the prognostic markers of chemoresistance. PUF60 (Poly (U)-binding splicing factor 60) is a nucleic acid-binding protein that has been shown to regulate transcription and link to tumorigenesis in various cancers. However, its biological role and function in chemotherapy resistance of GC is unclear. <b>Methods:</b> The expression and prognostic value of PUF60 in GC chemotherapy-resistant patients were analyzed by databases and K-M Plotter. The functional effect of PUF60 on chemoresistance in GC was studied by by RNA interference, CCK8 test, colony formation test and apoptosis detection. Moreover, further validation and mechanism exploration were conducted in clinical samples. <b>Results:</b> PUF60 was highly expressed in both GC and chemoresistant tissues, and was positively correlated with poor prognosis in GC patients treated with 5-fluorouracil (5-FU). In addition, the knockdown of PUF60 significantly reduced the proliferation of human gastric cancer cells and increased sensitivity to chemotherapy drugs, such as 5-FU and cisplatin (CDDP). Mechanistically, PUF60 enhances chemotherapy resistance in gastric cancer (GC) cells by actively excluding chemotherapy drugs via the recombinant ATP Binding Cassette Transporter A1 (ABCA1) and ATP Binding Cassette Subfamily C Member 1 (ABCC1). This process further affects the cell cycle, reduces cell apoptosis, and ultimately promotes resistance to chemotherapy in GC. <b>Conclusion:</b> PUF60 promotes chemoresistance in GC, resulting in poor prognosis of GC patients treated with 5-FU, and providing a new idea for overcoming the chemoresistance in GC.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 2","pages":"269-282"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11704696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142948465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}