International Journal of Medical Sciences最新文献

{"title":"Fructose Metabolism in Cancer: Molecular Mechanisms and Therapeutic Implications.","authors":"Xinyi Chen, Mu Yang, Lu Wang, Jingyao Tu, Xianglin Yuan","doi":"10.7150/ijms.108549","DOIUrl":"10.7150/ijms.108549","url":null,"abstract":"<p><p>Metabolic reprogramming enables cancer cells to adapt to the tumor microenvironment, facilitating their survival, proliferation, and resistance to therapy. While glucose has long been considered the primary substrate for cancer cell metabolism, recent studies have highlighted the role of fructose as an alternative carbon source. Fructose metabolism, particularly through key enzymes such as ketohexokinase (KHK) and aldolase B (ALDOB), along with the fructose transporter GLUT5, supports tumor growth, metastasis, and therapeutic resistance. This review explores the mechanisms by which fructose metabolism influences cancer progression, focusing on its metabolic pathways and its impact on the tumor microenvironment. By promoting glycolysis, lipid biosynthesis, and nucleotide production, fructose metabolism enhances the metabolic adaptability of cancer cells, especially in glucose-deprived conditions. A comprehensive understanding of these processes offers potential insights into therapeutic strategies targeting fructose metabolism for cancer treatment. However, further studies are required to fully elucidate the complex role of fructose in various malignancies.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 11","pages":"2852-2876"},"PeriodicalIF":3.2,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163614/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and Evaluation of tRF-Glu-CTC-013 as a Biomarker and Key Regulator in the Development of Cardiac Hypertrophy.","authors":"Wenlin Li, Ming Lan, Kun Xu, Sainan Li, Que Wang, Beidong Chen, Xiuqing Huang, Lin Dou, Na Jia, Li Zhao, Yuefeng Wang, Xingyun Jiao, Yong Man, Deping Liu, Liang Sun, Tong Zou, Qing He, Jian Li, Xue Yu, Tao Shen","doi":"10.7150/ijms.106114","DOIUrl":"10.7150/ijms.106114","url":null,"abstract":"<p><p>tRNA-derived small RNAs (tsRNAs) are a newly recognized class of non-coding RNAs involved in regulating RNA processing and translational control. Pathological cardiac hypertrophy, characterized by left ventricular remodeling under chronic stress, serves as a critical precursor to severe cardiovascular pathologies including myocardial ischemia, infarction, and heart failure. Utilizing an angiotensin II (Ang II)-induced mouse cardiac hypertrophy model combined with tsRNA transcriptome profiling, we identified differentially expressed tsRNAs and investigated their functional relevance. Validation in neonatal mouse ventricular myocytes (NMVMs) revealed five upregulated tsRNAs associated with hypertrophic progression. Functional characterization showed that overexpressing tRF-Glu-CTC-013 significantly reduced cardiomyocyte hypertrophy and inhibited inflammation and fibrosis. Further luciferase reporter assays revealed that tRF-Glu-CTC-013 could bind to the 3' UTR of TAS1R3, thereby inhibiting its expression and enhancing the level of autophagy in NMVMs. Taken together, these findings suggest that tsRNAs may act as novel regulators of cardiac remodeling, with tRF-Glu-CTC-013 emerging as a promising therapeutic candidate for cardioprotection via anti-hypertrophic, anti-inflammatory, and anti-fibrotic mechanisms.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 11","pages":"2839-2851"},"PeriodicalIF":3.2,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163609/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of cardiorespiratory fitness with adverse outcomes in patients with and without atrial fibrillation: a prospective cohort study.","authors":"Yutong Wang, Tao Xu, Chenxi Xia, Xinyang Song, Yanwen Chen, Sixian Weng, Fang Wang","doi":"10.7150/ijms.110802","DOIUrl":"10.7150/ijms.110802","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Background:&lt;/b&gt; Cardiorespiratory fitness plays a crucial role in cardiovascular health; however, its effects on adverse cardiovascular outcomes across different diseases remain poorly defined. Specifically, the differential impact of cardiorespiratory fitness on patients with and without atrial fibrillation (AF) is yet to be fully understood. This study aimed to explore the relationships between resting heart rate (RHR), maximal heart rate (HRmax), and maximal oxygen uptake (VO₂max) in relation to adverse cardiovascular outcomes, providing valuable insights to inform exercise prescriptions and cardiac rehabilitation practices. &lt;b&gt;Methods:&lt;/b&gt; Participants were classified into two groups: those with AF diagnosed prior to baseline (AF group) and those without AF at baseline (non-AF group). In the AF group, outcomes included heart failure (HF), stroke, and all-cause mortality; in the non-AF group, incident AF, stroke, HF, and mortality were assessed. Associations between cardiorespiratory indices-RHR, HRmax, and VO₂max-and adverse cardiovascular events were evaluated using Cox proportional hazards models. Dose-response relationships were examined via restricted cubic spline (RCS) models with three knots. &lt;b&gt;Results:&lt;/b&gt; In the non-AF population, higher resting heart rate was significantly associated with an increased risk of adverse cardiovascular outcomes, including heart failure (HF: HR = 1.008, 95% CI 1.001-1.014, P = 0.0182), stroke (HR = 1.010, 95% CI 1.004-1.016, P = 0.0018), atrial fibrillation (AF: HR = 1.011, 95% CI 1.007-1.015, P &lt; 0.0001), and all-cause mortality (HR = 1.016, 95% CI 1.010-1.022, P &lt; 0.0001). In contrast, higher HRmax was inversely associated with these outcomes (HF: HR = 0.993, 95% CI 0.991-0.995, P &lt; 0.0001; stroke: HR = 0.993, 95% CI 0.990-0.995, P &lt; 0.0001; AF: HR = 0.993, 95% CI 0.991-0.994, P &lt; 0.0001; cardiovascular death: HR = 0.994, 95% CI 0.990-0.997, P &lt; 0.0001). Similarly, higher cardiorespiratory fitness, measured by VO₂max, was consistently associated with reduced risks of adverse outcomes (HR range: 0.930-0.961, P &lt; 0.001). In the AF population, higher RHR was associated with elevated risks of HF (HR = 1.007, 95% CI 1.002-1.012, P = 0.0047) and all-cause mortality (HR = 1.009, 95% CI 1.004-1.014, P &lt; 0.0001). Conversely, greater VO₂max was linked to reduced risks of adverse outcomes, including HF (HR = 0.934, 95% CI 0.899-0.972, P &lt; 0.0001), stroke (HR = 0.943, 95% CI 0.891-0.999, P = 0.0446), and all-cause mortality (HR = 0.957, 95% CI 0.918-0.998, P = 0.038). &lt;b&gt;Conclusion:&lt;/b&gt; In individuals without AF, higher resting heart rate was significantly associated with increased risks of incident AF, HF, stroke, and all-cause mortality, with the lowest risks of AF and HF observed at an RHR of 61 beats per minute. Among patients with AF, elevated RHR was significantly linked to higher risks of HF and all-cause mortality. Conversely, higher VO₂max was consistently associated with reduced risks of adverse","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 11","pages":"2830-2838"},"PeriodicalIF":3.2,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PDGF-BB/EGR1 Axis Drives Fibroblast Activation Protein Expression to Promote Abdominal Aortic Aneurysm.","authors":"Zhihao Zhou, Lin Huang, Hui Luo, Rongzhou He, Ridong Wu, Rui Wang, Kangjie Wang, Chen Yao","doi":"10.7150/ijms.114429","DOIUrl":"10.7150/ijms.114429","url":null,"abstract":"<p><p>This study investigates the molecular mechanisms of fibroblast activation protein (FAP) in vascular smooth muscle cells (VSMCs) during abdominal aortic aneurysm (AAA) development. Bulk and single-cell RNA sequencing analysis revealed elevated FAP expression in AAA-derived VSMCs. In a porcine pancreatic elastase (PPE)-induced AAA mouse model, pharmacological inhibition of FAP (Ac-Gly-BoroPro) attenuated aneurysm formation and reduced macrophage infiltration. Further analysis showed that PDGF-BB upregulates FAP expression in VSMCs via the transcription factor EGR1, which binds to the FAP promoter to drive transcription. EGR1 inhibition significantly reduced PDGF-BB-induced FAP expression, highlighting its regulatory role. Additionally, clinical ¹⁸F-FAP inhibitor PET/CT imaging in an infectious AAA patient revealed strong FAP expression in the aneurysm wall. These findings underscore the importance of the PDGF-BB/EGR1/FAP axis in AAA pathogenesis and suggest that targeting FAP could offer therapeutic potential for managing AAA progression.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 11","pages":"2816-2829"},"PeriodicalIF":3.2,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163607/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations Between Long-Term Exposure to Air Pollutants and Prostate Cancer in a Large Taiwanese Population.","authors":"Jiun-Hung Geng, Chia-Yang Li, Ming-Tsang Wu, Szu-Chia Chen, Shu-Pin Huang","doi":"10.7150/ijms.109687","DOIUrl":"10.7150/ijms.109687","url":null,"abstract":"<p><p>Air pollution is associated with various illnesses including cancers, of which prostate cancer is one of the most prevalent malignancies in men. Emerging evidence has suggested that air pollution is a potential risk factor for prostate cancer. This study aimed to explore the relationship between air pollution and prostate cancer in a Taiwanese population. Using data from the Kaohsiung Medical University Hospital Database, we conducted a case-control study to identify patients with prostate cancer, and matched them by age with individuals without prostate cancer. Environmental pollution indices including particulate matter (PM), nitrogen oxides (NOx), sulfur dioxide (SO2), ozone (O3) and carbon monoxide (CO) were correlated with the patients' addresses using data from the Taiwan Central Air Quality Monitoring Network. The analysis included 3541 prostate cancer patients and 7082 age-matched controls. After adjusting for confounders, conditional logistic regression analysis demonstrated significant associations of prostate cancer with PM2.5 (odds ratio [95% confidence interval]: 1.240 [1.134-1.356]) and CO (odds ratio [95% confidence interval]: 1.105 [1.025-1.192]) at the index date, with similar associations observed for average exposure levels over 1, 2, 3, and 5 years prior to the index date. Furthermore, sensitivity analyses revealed that the odds ratios for combined-risk Z-score exposure at the index date and over these same time periods were 1.029, 1.033, 1.034, 1.034, and 1.033, respectively. These findings suggest that prolonged exposure to multiple air pollutants collectively contributes to prostate cancer risk. Further investigations are needed to validate these findings and explore potential underlying mechanisms.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 11","pages":"2771-2781"},"PeriodicalIF":3.2,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development Of the VAMPCT Score for Predicting Mortality in CKD Patients with COVID-19.","authors":"Chaofan Li, Yue Niu, Xinyan Pan, Dinghua Chen, Fei Liu, Zhe Feng, Yong Wang, Xueying Cao, Jie Wu, Jiabao Liu, Xin Guan, Xuefeng Sun, Li Zhang, Guangyan Cai, Xiangmei Chen, Ping Li","doi":"10.7150/ijms.111558","DOIUrl":"10.7150/ijms.111558","url":null,"abstract":"<p><p><b>Background:</b> Chronic kidney disease (CKD) patients with coronavirus disease 2019 (COVID-19) are at significant risk of death. However, clinical identification of high-risk individuals remains suboptimal despite the recognition of many pathophysiological and comorbidity-related risk factors. We aim to develop a clinically simple machine learning (ML)-based score to predict acute COVID-19 mortality among CKD patients. <b>Methods:</b> CKD inpatients with COVID-19 were prospectively enrolled from December 2022 to January 2023 with a three-month follow-up. Feature selection from clinical and laboratory results was performed through least absolute shrinkage and selection operator and stepwise selection. Logistic regression, support vector machine (SVM), random forest, and extreme gradient boosting were applied for ML model development. A predictive score for mortality was constructed using logistic regression. We compared predictive ability between the proposed score and other published scores. <b>Results:</b> 219 CKD patients were included and had a high mortality rate of 25.1%. The SVM model exhibited the best performance, with the validation area under the receiver operating characteristic curve (AUC) being 0.946 (95% CI 0.918, 0.974). The COVID-19 vaccination status, age, monocyte percentage, prothrombin activity, cardiac troponin T, and total bilirubin (\"VAMPCT\") were the most relevant factors and utilized to develop the scoring system with an AUC of 0.960 (95% CI 0.935, 0.985). <b>Conclusion:</b> ML models predicting three-month mortality had favorable performance for CKD patients with COVID-19. The VAMPCT mortality score provided a user-friendly approach.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 11","pages":"2782-2791"},"PeriodicalIF":3.2,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Large Language Models in Medicine: Applications, Challenges, and Future Directions.","authors":"Erlan Yu, Xuehong Chu, Wanwan Zhang, Xiangbin Meng, Yaodong Yang, Xunming Ji, Chuanjie Wu","doi":"10.7150/ijms.111780","DOIUrl":"10.7150/ijms.111780","url":null,"abstract":"<p><p>In recent years, large language models (LLMs) represented by GPT-4 have developed rapidly and performed well in various natural language processing tasks, showing great potential and transformative impact. The medical field, due to its vast data information as well as complex diagnostic and treatment processes, is undoubtedly one of the most promising areas for the application of LLMs. At present, LLMs has been gradually implemented in clinical practice, medical research, and medical education. However, in practical applications, medical LLMs still face numerous challenges, including the phenomenon of hallucination, interpretability, and ethical concerns. Therefore, in-depth exploration is still needed in areas of standardized evaluation frameworks, multimodal LLMs, and multidisciplinary collaboration in the future, so as to realize the widespread application of medical LLMs and promote the development and transformation in the field of global healthcare. This review offers a comprehensive overview of applications, challenges, and future directions of LLMs in medicine, providing new insights for the sustained development of medical LLMs.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 11","pages":"2792-2801"},"PeriodicalIF":3.2,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163604/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Acupuncture in Managing Radiation-Induced Xerostomia: An Updated Meta-Analysis.","authors":"Ru-Yin Tsai, Sheng-Yi Lin, Chin-Chang Chen, Yao Hsiao","doi":"10.7150/ijms.110366","DOIUrl":"10.7150/ijms.110366","url":null,"abstract":"<p><p><b>Background:</b> Xerostomia, or dry mouth, frequently affects head and neck cancer patients receiving radiotherapy, leading to discomfort and impacting daily functions such as speaking and swallowing. Conventional treatments may offer limited relief and are often accompanied by undesirable side effects. Acupuncture, as a non-pharmacological intervention, is increasingly explored for its potential to mitigate xerostomia symptoms. <b>Objective:</b> This systematic review and meta-analysis aim to assess the effectiveness of acupuncture in improving symptoms and quality of life in patients experiencing radiation-induced xerostomia. <b>Methods:</b> A thorough literature search was conducted across several databases, including MEDLINE, Embase, Cochrane Central, and Web of Science, up to the current year. We included randomized controlled trials (RCTs) that evaluated acupuncture's impact on salivary flow and symptom relief in adults with radiation-induced xerostomia. Primary outcomes were changes in salivary flow, with secondary outcomes including patient-reported symptom severity and quality of life metrics. The risk of bias was evaluated, and data were synthesized using a random-effects model. <b>Results:</b> A total of 11 RCTs involving 1271 participants were included in the analysis. The pooled data showed a moderate increase in salivary flow in the acupuncture group, effective in both resting and stimulated conditions. Additionally, acupuncture demonstrated significant benefits in reducing xerostomia symptoms and improving quality of life scores compared to control interventions. Subgroup analysis revealed that traditional acupuncture was more effective than Transcutaneous Electrical Nerve Stimulation (TENS). <b>Conclusions:</b> The findings suggest that acupuncture may be an effective complementary treatment for radiation-induced xerostomia, offering relief from dry mouth symptoms and potentially improving quality of life. Further research should focus on standardizing acupuncture protocols to confirm these benefits across diverse patient populations.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 11","pages":"2802-2815"},"PeriodicalIF":3.2,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Value of the Combined Detection of Microbiota, Multiple Inflammation-Related Indicators, Serum Lipid Indices, and Tumour Markers in Colorectal Polyps: A Case-Control Study.","authors":"Limin Zhang, Guoxiu Xiao, Duyao Su, Xun Wang, Cuiting Lv, Chunchun Li, Mingsheng Fu, Liang Song","doi":"10.7150/ijms.108819","DOIUrl":"10.7150/ijms.108819","url":null,"abstract":"<p><p><b>Purpose:</b> Colorectal polyps have few clinical symptoms, and related tumor markers are unclear; therefore, developing a simple and economical tumor detection index for auxiliary diagnosis is necessary. We aimed to investigate differences in salivary and fecal microbiota, inflammation-related indicators, serum lipid indices, and tumor markers between patients with colorectal polyps and healthy controls, to identify novel non-invasive biomarkers for colorectal polyps. <b>Patients and methods:</b> This case-control study enrolled 47 patients with colorectal polyps and 59 age- and sex-matched healthy controls between 13 May 2022 and 20 November 2023. From each participant, we collected salivary and fecal samples, fasting venous blood samples, polyp tissues, and normal intestinal tissues. We then evaluated the diagnostic performance of multiple markers, including salivary and fecal microbiota, routine blood tests, blood lipids, serum tumor markers, and the NOD-like receptor protein 3 (NLRP3) inflammasome, both individually and in combination. The assessment was based on metrics such as the Youden index, sensitivity, and specificity. <b>Results:</b> There were statistically significant differences in several markers between patients and controls. The receiver operating characteristic curve analysis showed that the areas under the curve for the diagnosis of colorectal polyps using the individual and combined detection of the neutrophil-to-lymphocyte ratio, mean corpuscular haemoglobin (MCH), MCH concentration, cystatin C, triglycerides, low-density lipoprotein cholesterol, carcinoembryonic antigen, <i>Porphyromonas gingivalis</i>, <i>Fusobacterium nucleatum</i>, <i>Prevotella intermedia</i>, <i>Ruminococcus gnavus</i>, <i>Bacteroides ovatus</i>, and <i>Parabacteroides distasonis</i> were 0.696, 0.726, 0.742, 0.771, 0.829, 0.731, 0.785, 0.759, 0.738, 0.786, 0.739, 0.764, 0.757, and 0.996, respectively. Combining 13 markers was better than a single marker regarding the diagnostic effect. Compared to that in normal mucosal tissues, the ratio of positively stained areas for NLRP3, apoptosis-associated speck-like proteins containing a caspase recruitment domain, and interleukin-1β was higher in polyp tissues. <b>Conclusion:</b> Detection of salivary and fecal microbiota, multiple inflammation-related indicators, serum lipid indices, and tumor markers can non-invasively and effectively improve the diagnosis of colorectal polyps.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 11","pages":"2757-2770"},"PeriodicalIF":3.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163605/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ABCG1 promotes the proliferation and migration of clear cell renal cell carcinoma and reduces its apoptosis.","authors":"Yihan Dong, Qiufang Qiao, Shaomin Guo, Ruibing Chen, Tianyu Lin, Xinyu Liu, Jiaxin Li, Shiming Liu, Huamao Jiang, Yong Wang, Dan Yue, Rui Wang","doi":"10.7150/ijms.107055","DOIUrl":"10.7150/ijms.107055","url":null,"abstract":"<p><p><b>Background and Aims:</b> In this study, we analyzed the expression levels of ATP-binding cassette transporter G1 (ABCG1) in clear cell renal cell carcinoma (ccRCC) to assess its significance for early diagnosis and its role in the disease's biological processes. <b>Materials and Methods:</b> ABCG1 expression levels in ccRCC were determined by analyzing The Cancer Genome Atlas (TCGA) database, as well as immunohistochemical staining and western blot. The correlation between the ABCG1 expression level and the clinicopathological stage and diagnostic prognosis of ccRCC were analyzed by the TCGA database. The ABCG1 stable knockout cell line and functional experiments using ABCG1 inhibitors were constructed to verify the effect of reduced ABCG1 expression on the ccRCC cell function. In addition, the pathways and genes affected by ABCG1 were analyzed by RNA-seq. <b>Results:</b> Analysis of the TCGA database demonstrated, as well as immunohistochemical staining and western blot detection, confirmed that ABCG1 was significantly elevated in ccRCC. The expression level of ABCG1 was correlated with the clinicopathological stage of ccRCC. The Cox regression analysis showed that ABCG1 could be used as an independent prognostic marker for ccRCC. And decreasing ABCG1 expression decreased the proliferation, migration, and invasion abilities of ccRCC cells, with increased apoptosis. In addition, analysis of ABCG1-related differentially expressed genes (DEGs) showed that ABCG1 positively regulates specific proliferation-related genes and negatively affects those associated with apoptosis. <b>Conclusions:</b> In summary, these findings highlight the critical role of ABCG1 in ccRCC progression and suggest its potential as a biomarker for diagnosis and prognosis.</p>","PeriodicalId":14031,"journal":{"name":"International Journal of Medical Sciences","volume":"22 11","pages":"2721-2737"},"PeriodicalIF":3.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}