International journal of medicinal mushrooms最新文献

{"title":"Enhancement of Cordycepin Production Through Liquid Static Fermentation of Caterpillar Medicinal Mushroom Cordycep militaris (Ascomycetes)","authors":"Mahesh Borde, Shweta Kumawat, Sanjay K. Singh","doi":"10.1615/intjmedmushrooms.2024055323","DOIUrl":"https://doi.org/10.1615/intjmedmushrooms.2024055323","url":null,"abstract":"Cordycepin (3′-deoxyadenosine) is a bioactive nucleoside analogue synthesise by Cordyceps militaris. Liquid fermentation of C. militaris by addition in different concentrations of five additives singly was evaluated. glycine at 15.00 g/L after 20 days enhanced the cordycepin of 1773.33 mg/L (15-fold increment over control). adenine at 4.00 g/L and 6.00 g/L in the liquid media showed significantly higher cordycepin i.e.1596.66 mg/L and 1550.00 mg/L (3-fold increment over control) after 40 days. Tryptone supplementation 14.00 g/L significantly higher cordycepin 784.33 mg/L (6.70-fold increment over control) and 912.66 mg/L production after 20 and 40 days of inoculation. Peanut oil at 10.00 g/L produced 585.66 mg/L (5-fold increment over control) cordycepin after 20 days and after 40 days, also addition of Peanut oil at 20.00 g/L and 30.00 g/L in the media showed 631.66 and 624.31 mg/L cordycepin content. Supplementation of monosodium glutamate at 0.30 g/L produced significantly highest cordycepin i.e. 614 mg/L and 635.00 mg/L cordycepin after 20 and 40 days respectively.","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141932850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hot Water Extract of Caterpillar Medicinal Mushroom Cordyceps militaris (Ascomycetes) Fruiting Body Activates Myeloid Dendritic Cells through a Dectin-1-Mediated Pathway","authors":"Takashi Kanno, Rui Tada, Toyokazu Nakasone, Shigemi Okamatsu, Yoichiro Iwakura, Kazuhiro Tamura, Hiroaki Miyaoka, Yoshiyuki Adachi","doi":"10.1615/intjmedmushrooms.2024055210","DOIUrl":"https://doi.org/10.1615/intjmedmushrooms.2024055210","url":null,"abstract":"The traditional use of Cordyceps militaris, an entomopathogenic fungus, in East Asian medicine has been well documented. Our previous study revealed that the fruiting body powder of C. militaris, referred to as Ryukyu-kaso, contains 1,3-β-glucan and stimulates bone marrow-derived dendritic cells via a dectin-1-dependent pathway. However, the immunomodulatory effects of soluble 1,3-β-glucan in Ryukyu-kaso and the underlying mechanisms remain unclear. In the present study, we aimed to investigate the immunostimulatory effects of the hot water extract of C. militaris fruiting body on bone marrow-derived dendritic cells and the involvement of the β-glucan receptor dectin-1. Our findings revealed that the hot water extract of C. militaris fruiting body contains soluble 1,3-β-glucan and potently induces bone marrow-derived dendritic cells to secrete both pro-inflammatory and immunoregulatory cytokines. RK-HWE significantly increased the secretion of TNF-α, IL-6, IFN-β, IL-12p70, and GM-CSF compared to control (all p < 0.05). For instance, TNF-α secretion increased from 7.82 ± 1.57 pg/mL in control to 403.7 ± 59.7 pg/mL with RK-HWE stimulation (p < 0.001). Notably, these immunostimulatory effects of hot water extract of C. militaris fruiting body were completely abolished in bone marrow-derived dendritic cells derived from dectin-1-knockout mice (p < 0.001 for all cytokines), suggesting that dectin-1 is essential for immunomodulation induced by hot water extract of C. militaris fruiting body. These findings provide novel insights into the mechanisms underlying the immunostimulatory effects of hot water extract of C. militaris fruiting body and highlight its potential as a natural immunomodulatory agent for various therapeutic applications.","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141872908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory Effects of the Methanol Extract of the Golden Chanterelle Mushroom Cantharellus cibarius (Agaricomycetes) Against Human Acute Myeloid Leukemia Cell Line","authors":"Zhihong Xu, Antonio Shall, Eric Dadey, Qiong Wu, Zhijian Qian","doi":"10.1615/intjmedmushrooms.2024055328","DOIUrl":"https://doi.org/10.1615/intjmedmushrooms.2024055328","url":null,"abstract":"Currently available treatments for acute myeloid leukemia exhibit side effects that limit their use, with primary and secondary resistance as persistent issues. While edible mushrooms possess nutritional value, they are also an excellent source of bioactive compounds that may have the potential to treat multiple disease states. The aim of the present study was to investigate the in vitro inhibitory effects of chromatographic fractions from the methanol extract of Cantharellus cibarius in a human acute myeloid leukemia (AML) cell line MV4-11. The mushrooms were purchased from a grocery store, the fruiting bodies were triturated and then extracted with 99.9% methanol to generate a crude extract. This crude extract was then redissolved in methanol, filtered over cotton to remove insoluble solids, and then fractionated over Sephadex® LH-20. Each fraction was dried and then analyzed by reverse-phase HPLC. A typical UV wavelength was selected for the detection of possible anticancer compounds in C. cibarius based on the major chromophores in main fungal anticancer agents reported. Based on chromatographic profiles, specific fractions that might contain potential anticancer agents were combined, and the inhibitory activity of the combined fractions was assessed against the MV4-11 leukemia cell line. Data confirmed that one combined fraction LH-20F-IV showed the greatest degree of in vitro inhibitory activity against the selected cell line. Fast determination of the potential anticancer compound containing fraction was completed through the application of reported chemoinformatics on HPLC detection of possible chromophores in potential anticancer agents, in combination with cellular bioassays.","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141932916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Silico Investigation of Suillin Derived from Suillus luteus Mushroom (Agaricomycetes), Targeting Acetylcholinesterase: Docking and Virtual Screening Study","authors":"ADITYA GANESHPURKAR, Ankit Ganeshpurkar, Pushpendra Kumar Khangar, Likhit Akotkar, Swati Jain, Harshal Prajapati, Nazneen Dubey","doi":"10.1615/intjmedmushrooms.2024055215","DOIUrl":"https://doi.org/10.1615/intjmedmushrooms.2024055215","url":null,"abstract":"This study integrates bioinformatics and computer-aided drug discovery to assess Suillin's therapeutic potential, particularly its interaction with acetylcholinesterase (AChE). Alzheimer's disease presents profound challenges, necessitating effective treatments to mitigate cognitive decline and improve patient quality of life. While current medications offer symptomatic relief, they often entail adverse effects and do not address the\u0000underlying disease progression. Natural sources, such as macrofungi mushrooms, hold promise for novel drug discovery due to their bioactive compounds; diverse therapeutic properties. Suillin, derived from Suillus luteus mushrooms, shows promise as a mixed-type AChE inhibitor, crucial for maintaining acetylcholine levels in neurodegenerative disorders like Alzheimer's disease. Computational docking studies reveal suillin's distinctive interactions with AChE, suggesting potential modulation of enzyme function through various bonding mechanisms. The Molinspiration drug-likeness score further supports Suillin's efficacy, indicating its suitability for enzyme inhibition. By combining computational and bioinformatics approaches, this study elucidates suillin's molecular interactions and underscores its potential as a therapeutic agent.","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141932914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Mycelial Growth Parameters in Lion’s Mane Edible Medicinal Mushroom Hericium erinaceus (Agaricomycetes) by Supplementation of Growth Hormones","authors":"Stanzin Disket, Sachin Gupta, Moni Gupta, Pavleen Kour, Laxmi Devi, Ranbir Singh","doi":"10.1615/intjmedmushrooms.2024055204","DOIUrl":"https://doi.org/10.1615/intjmedmushrooms.2024055204","url":null,"abstract":"The present study was carried out to optimize the strain and evaluate the effect\u0000of amendment of growth media with different hormone concentrations for enhancing\u0000mycelium growth of lion’s mane mushroom (Hericium erinaceus) under in vitro conditions. Among the five strains of H. erinaceus, He-04 strain showed maximum GRavr of 4.78 mm d-1 . Different media viz., potato dextrose agar (PDA), malt extract agar (MEA), sawdust extract agar (SDEA), wheat straw extract agar (WSEA) and rice straw extract agar (RSEA) amended with four concentrations (10, 20, 30 and 40 ppm) of gibberellic acid, kinetin and indole acetic acid were evaluated for promotion of mycelial growth of H. erinaceus. PDA was observed to be the best media promoting the mycelial growth of H. erinaceus. The highest mycelial GRavr 8.47 mm d -1 was observed in PDA amended with indole acetic acid (10 ppm) followed by gibberellic acid and kinetin (30 ppm) decreasing mycelial GRav to 8.15 and 7.75mm d -1 respectively. Temperature of 25°C and pH 7.0 was found to be the best for mycelium growth of H. erinaceus.","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141866136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) Supplementation on Psychological Stress and Selective Fitness Profile Parameters in Female College Students in West Bengal, India","authors":"Sudeep Mitra, Mousumi Mitra, Dilip Kumar Nandi, Mantu Saha, Amit Bandyopadhyay","doi":"10.1615/intjmedmushrooms.2024055300","DOIUrl":"https://doi.org/10.1615/intjmedmushrooms.2024055300","url":null,"abstract":"Psychological disparities impact physical activity and fitness in sedentary female college students by affecting cardiovascular efficiency. Ganoderma lucidum, vitality-enhancing herb alleviates health and rejuvenates the mind-body to improve endurance fitness. A double-blinded, randomized, placebo-controlled parallel design study was conducted to determine whether supplementation of G. lucidum in daily dosages of 500 mg and 1000 mg improves psychophysiological health capabilities during the different phases of the experimental trial. Analysis for pre-experimental trial (0 day), experimental trial (15thday), and post-experimental trial (after 30th day) on anthropometric, psychological, physiological and physical fitness parameters were executed. Seventy-eight participants (n=78, age=20.64±3.21 years) were assigned randomly and equally divided (n=26) to one of the three treatment groups for intragroup and intergroup comparisons. Significant differences in the post-experimental G. lucidum1000mg supplementation group for heart rate (HR), maximal oxygen consumption (VO2max), physical work capacity (PWC170) and right-hand grip strength (p<0.05) in comparison to the placebo group were observed. G. lucidum1000mgsupplemented group also significantly improved (p<0.05) HR, VO2 max and PWC170 (p<0.001) after pre- to post-trials. Experimental trial between placebo and G.lucidum1000mg group and post-experimental trial between G. lucidum500mg and G. lucidum1000mg group showed significant changes in VO2max (p<0.001) and PWC170 (p<0.05). Anxiety, depression, vitality and positive wellbeing scores significantly improved, leading to improved psychological well-being after G. lucidum1000mg supplementation. G. lucidum1000mg supplementation for 30 days might act as a longevity-promoting tonic for endurance and strength performance by ameliorating stress to improve the overall psychophysiological health, vitality and quality of life for better cardiovascular efficacy.","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141932913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitin and Its Derivative Chitosan: Distribution in Nature, Applications Realm, and Technology Research (A Review)","authors":"Vladimir Perelygin, Mikhail Zharikov, Ivan Zmitrovich, Tatyana Nekrasova","doi":"10.1615/intjmedmushrooms.2024055012","DOIUrl":"https://doi.org/10.1615/intjmedmushrooms.2024055012","url":null,"abstract":"The present review highlights the chitin/chitosan as biopolymers that are promising for biomedical research development. Our goal was to assess the potential for practical use of these biopolymers and to summarize information on traditional and innovative technologies for their production and purification. The widespread occurrence of chitin and chitosan in nature as well as the unique chemical and biological properties of chitosan are reasons of growing interest in the use of the latter in several pharmaceutical fields. The main stages of chitin extraction and its further modification into chitosan are deproteinization, demineralization, deacetylation, and the main methods of chitosan purification are filtration, dialysis and reprecipitation. The profitability of the production of chitin/chitosan from crustaceans and edible mushrooms is approximately at the same level. The cost of mushroom products can be reduced by using agricultural or forestry waste as nutrient substrates. This makes the use of fungi as sources of chitin/chitosan in forested regions a rather promising issue.","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141737171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Medicinal Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) Sclerotia Supplementation Mitigates Hypertension and Alleviates Vascular Dysfunction Partly Through Oxidative Stress Modulation in Spontaneously Hypertensive Rats","authors":"Yi En Wong, Muhammad Fazril Mohamad Razif, Szu-Ting Ng, Chon-Seng Tan, Shin Yee Fung, Dharmani Murugan","doi":"10.1615/intjmedmushrooms.2024055061","DOIUrl":"https://doi.org/10.1615/intjmedmushrooms.2024055061","url":null,"abstract":"Hypertension is a risk factor for cardiovascular diseases such as coronary artery disease, heart failure and stroke. Lignosus rhinocerus (Cooke) Ryvarden (also known as tiger milk mushroom) has been reported to exhibit a range of pharmacological effects, such as anti-inflammatory, anti-proliferative, antioxidative, immunomodulatory and anti-asthmatic activities. Thus far, limited research has explored its ability to mediate vascular effects in vivo. Therefore, this study investigated the antihypertensive and vascular protective effects of L. rhinocerus TM02® sclerotia supplementation in spontaneously hypertensive rats (SHR). Wistar Kyoto (WKY) rats served as a normotensive control group. SHR were orally administered with L. rhinocerus TM02® sclerotia (100 mg/kg and 300 mg/kg, respectively) for 8 weeks, and blood pressure was monitored every 2 weeks. Vascular function was evaluated using an organ bath (aorta) and wire myograph (renal artery) at the treatment endpoint. The levels of reactive oxygen species (ROS) and nitric oxide (NO) in the aorta and renal artery were evaluated using dihydroethidium (DHE) and difluoro fluorescein acetate (DAF-FM) fluorescence assays, respectively. Total plasma nitrate/nitrite and tumor necrosis factor alpha (TNF-α) levels were evaluated via colorimetric assays. In vivo treatment with L. rhinocerus TM02® sclerotia significantly attenuated the increase in systolic blood pressure (SBP). It also alleviated vascular dysfunction and decreased elevated ROS in the aorta and renal arteries of the treated SHRs. Moreover, L. rhinocerus TM02® sclerotia attenuated plasma TNF-α level but increased total plasma nitrate/nitrite, albeit slightly, coupled with significantly increased NO at the vascular level. Collectively, the present study demonstrated that L. rhinocerus TM02® sclerotia supplementation exerted blood pressure lowering effects, partly attributed to improvements in vascular function via reduction in vascular oxidative stress.","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141775677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Influence of Polysaccharide Fractions of the Lion’s Mane Medicinal Mushroom Hericium erinaceus (Agaricomycetes) on the Immunomodulatory and Antioxidant Activity of Neutrophils Exposed to Stress of Different Durations","authors":"Oksana Zaitseva, Marta Sergushkina, Dmitry Popyvanov, Yanina Nazarova, Tatyana Polezhaeva, Olga Solomina, Andrey Khudyakov","doi":"10.1615/intjmedmushrooms.2024054937","DOIUrl":"https://doi.org/10.1615/intjmedmushrooms.2024054937","url":null,"abstract":"Polysaccharide fractions from the mycelium of the fungus Hericium erinaceus BP 16, cultivated on sterile grain substrates (barley, oats, wheat, rice, rye), were isolated and characterized. 1% solutions were prepared from the resulting fractions, mixed with a blood, which was then subjected to cold stress at a temperature of 6°C for 3, 5 and 7 days. It has been shown that the fraction of H. erinaceus grown on rye is characterized by a high content of the protein fraction and arabinose monosaccharide and contributes to the preservation of higher phagocytic, bactericidal and antioxidant activity cells throughout the entire period of stress. Polysaccharide fractions of the fungus H. erinaceus, grown on various grain substrates, can serve as an immunomodulatory and antioxidant food additive and provide significant benefits in the daily life of people with stress and reduced immunity.","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141721171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Comparative Transcriptome and Weighted Gene Co-Expression Network Analysis Provide Valuable Insights into the Mechanisms of Pinhead Initiation in Chinese Cordyceps Ophiocordyceps sinensis (Ascomycota)","authors":"He Li, Fang Xiao, Chen Xi Dou, Bo Zhou, Zhao He Chen, Jing Yi Wang, Cheng Gang Wang, Fang Xie","doi":"10.1615/intjmedmushrooms.2024054674","DOIUrl":"https://doi.org/10.1615/intjmedmushrooms.2024054674","url":null,"abstract":"ABSTRACT: The initiation and formation of the pinhead is the key node in growth process of Ophiocordyceps sinensis (Chinese Cordyceps). The research on the mechanism of changes in this growth stage is the basis for realizing the industrialization of its artificial cultivation. Clarifying the mechanisms of pinhead initiation is essential for its further application. Here, we performed a comprehensive transcriptome analysis of pinhead initiation process in O. sinensis. Comparative transcriptome analysis revealed remarkable variation in gene expression and enriched pathways at different pinhead initiation stages. Gene co-expression network analysis by WGCNA identified 4 modules highly relevant to different pinhead initiation stages, and 23 hub genes. The biological function analysis and hub gene annotation of these identified modules demonstrated that transmenbrance transport and nucleotide excision repair were the topmost enriched in pre-pinhead initiation stage, carbohydrate metabolism and protein glycosylation were specially enriched in pinhead initiation stage, nucleotide binding and DNA metabolic process were over-represented after pinhead stage. These key regulators are mainly involved in carbohydrate metabolism, synthesis of proteins and nucleic acids. This work excavated the candidate pathways and hub genes related to the pinhead initiation stage, which will serve as a reference for realizing the industrialization of artificial cultivation in O. sinensis.","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141504748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}