金黄鸡油菌(姬松茸)甲醇提取物对人类急性髓性白血病细胞株的抑制作用

IF 1.4 4区 生物学 Q4 MYCOLOGY
Zhihong Xu, Antonio Shall, Eric Dadey, Qiong Wu, Zhijian Qian
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引用次数: 0

摘要

目前现有的急性髓性白血病治疗方法所表现出的副作用限制了它们的使用,原发性和继发性抗药性是长期存在的问题。食用菌具有营养价值,同时也是生物活性化合物的极佳来源,可能具有治疗多种疾病的潜力。本研究的目的是调查从西巴瑞菇甲醇提取物中提取的色谱馏分对人类急性髓性白血病(AML)细胞系 MV4-11 的体外抑制作用。蘑菇是从杂货店购买的,子实体经三叉处理后用 99.9% 的甲醇提取,生成粗提取物。然后将粗提取物重新溶于甲醇,用棉花过滤以去除不溶性固体,再用 Sephadex® LH-20 进行分馏。每个馏分干燥后采用反相高效液相色谱法进行分析。根据已报道的主要真菌抗癌剂中的主要发色团,选择了一种典型的紫外波长来检测 Cibarius 中可能的抗癌化合物。根据色谱图,合并了可能含有潜在抗癌剂的特定馏分,并评估了合并馏分对 MV4-11 白血病细胞系的抑制活性。数据证实,一种组合馏分 LH-20F-IV 对所选细胞株的体外抑制活性最强。通过应用已报道的化学信息学方法对潜在抗癌剂中可能存在的发色团进行高效液相色谱检测,并结合细胞生物测定,快速确定了含有潜在抗癌化合物的馏分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Inhibitory Effects of the Methanol Extract of the Golden Chanterelle Mushroom Cantharellus cibarius (Agaricomycetes) Against Human Acute Myeloid Leukemia Cell Line
Currently available treatments for acute myeloid leukemia exhibit side effects that limit their use, with primary and secondary resistance as persistent issues. While edible mushrooms possess nutritional value, they are also an excellent source of bioactive compounds that may have the potential to treat multiple disease states. The aim of the present study was to investigate the in vitro inhibitory effects of chromatographic fractions from the methanol extract of Cantharellus cibarius in a human acute myeloid leukemia (AML) cell line MV4-11. The mushrooms were purchased from a grocery store, the fruiting bodies were triturated and then extracted with 99.9% methanol to generate a crude extract. This crude extract was then redissolved in methanol, filtered over cotton to remove insoluble solids, and then fractionated over Sephadex® LH-20. Each fraction was dried and then analyzed by reverse-phase HPLC. A typical UV wavelength was selected for the detection of possible anticancer compounds in C. cibarius based on the major chromophores in main fungal anticancer agents reported. Based on chromatographic profiles, specific fractions that might contain potential anticancer agents were combined, and the inhibitory activity of the combined fractions was assessed against the MV4-11 leukemia cell line. Data confirmed that one combined fraction LH-20F-IV showed the greatest degree of in vitro inhibitory activity against the selected cell line. Fast determination of the potential anticancer compound containing fraction was completed through the application of reported chemoinformatics on HPLC detection of possible chromophores in potential anticancer agents, in combination with cellular bioassays.
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来源期刊
CiteScore
2.60
自引率
16.70%
发文量
91
审稿时长
6-12 weeks
期刊介绍: The rapid growth of interest in medicinal mushrooms research is matched by the large number of disparate groups that currently publish in a wide range of publications. The International Journal of Medicinal Mushrooms is the one source of information that will draw together all aspects of this exciting and expanding field - a source that will keep you up to date with the latest issues and practice. The International Journal of Medicinal Mushrooms published original research articles and critical reviews on a broad range of subjects pertaining to medicinal mushrooms, including systematics, nomenclature, taxonomy, morphology, medicinal value, biotechnology, and much more.
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