Enhancement of Cordycepin Production Through Liquid Static Fermentation of Caterpillar Medicinal Mushroom Cordycep militaris (Ascomycetes)

Abstract

Cordycepin (3′-deoxyadenosine) is a bioactive nucleoside analogue synthesise by Cordyceps militaris. Liquid fermentation of C. militaris by addition in different concentrations of five additives singly was evaluated. glycine at 15.00 g/L after 20 days enhanced the cordycepin of 1773.33 mg/L (15-fold increment over control). adenine at 4.00 g/L and 6.00 g/L in the liquid media showed significantly higher cordycepin i.e.1596.66 mg/L and 1550.00 mg/L (3-fold increment over control) after 40 days. Tryptone supplementation 14.00 g/L significantly higher cordycepin 784.33 mg/L (6.70-fold increment over control) and 912.66 mg/L production after 20 and 40 days of inoculation. Peanut oil at 10.00 g/L produced 585.66 mg/L (5-fold increment over control) cordycepin after 20 days and after 40 days, also addition of Peanut oil at 20.00 g/L and 30.00 g/L in the media showed 631.66 and 624.31 mg/L cordycepin content. Supplementation of monosodium glutamate at 0.30 g/L produced significantly highest cordycepin i.e. 614 mg/L and 635.00 mg/L cordycepin after 20 and 40 days respectively.