Hot Water Extract of Caterpillar Medicinal Mushroom Cordyceps militaris (Ascomycetes) Fruiting Body Activates Myeloid Dendritic Cells through a Dectin-1-Mediated Pathway

IF 1.4 4区 生物学 Q4 MYCOLOGY
Takashi Kanno, Rui Tada, Toyokazu Nakasone, Shigemi Okamatsu, Yoichiro Iwakura, Kazuhiro Tamura, Hiroaki Miyaoka, Yoshiyuki Adachi
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引用次数: 0

Abstract

The traditional use of Cordyceps militaris, an entomopathogenic fungus, in East Asian medicine has been well documented. Our previous study revealed that the fruiting body powder of C. militaris, referred to as Ryukyu-kaso, contains 1,3-β-glucan and stimulates bone marrow-derived dendritic cells via a dectin-1-dependent pathway. However, the immunomodulatory effects of soluble 1,3-β-glucan in Ryukyu-kaso and the underlying mechanisms remain unclear. In the present study, we aimed to investigate the immunostimulatory effects of the hot water extract of C. militaris fruiting body on bone marrow-derived dendritic cells and the involvement of the β-glucan receptor dectin-1. Our findings revealed that the hot water extract of C. militaris fruiting body contains soluble 1,3-β-glucan and potently induces bone marrow-derived dendritic cells to secrete both pro-inflammatory and immunoregulatory cytokines. RK-HWE significantly increased the secretion of TNF-α, IL-6, IFN-β, IL-12p70, and GM-CSF compared to control (all p < 0.05). For instance, TNF-α secretion increased from 7.82 ± 1.57 pg/mL in control to 403.7 ± 59.7 pg/mL with RK-HWE stimulation (p < 0.001). Notably, these immunostimulatory effects of hot water extract of C. militaris fruiting body were completely abolished in bone marrow-derived dendritic cells derived from dectin-1-knockout mice (p < 0.001 for all cytokines), suggesting that dectin-1 is essential for immunomodulation induced by hot water extract of C. militaris fruiting body. These findings provide novel insights into the mechanisms underlying the immunostimulatory effects of hot water extract of C. militaris fruiting body and highlight its potential as a natural immunomodulatory agent for various therapeutic applications.
毛孢药用蘑菇冬虫夏草(子囊菌科)子实体的热水提取物通过 Dectin-1 介导的途径激活髓系树突状细胞
冬虫夏草是一种昆虫病原真菌,它在东亚医学中的传统用途已有详细记载。我们之前的研究发现,被称为琉球卡索的冬虫夏草子实体粉末含有 1,3-β-葡聚糖,能通过依赖于右旋糖酐-1 的途径刺激骨髓树突状细胞。然而,琉球卡索中可溶性 1,3-β 葡聚糖的免疫调节作用及其内在机制仍不清楚。在本研究中,我们旨在研究琉球菰子实体热水提取物对骨髓树突状细胞的免疫刺激作用以及β-葡聚糖受体 dectin-1 的参与。我们的研究结果表明,绿僵菌子实体的热水提取物含有可溶性 1,3-β 葡聚糖,能有效诱导骨髓树突状细胞分泌促炎和免疫调节细胞因子。与对照组相比,RK-HWE 能明显增加 TNF-α、IL-6、IFN-β、IL-12p70 和 GM-CSF 的分泌(所有 p 均为 0.05)。例如,在 RK-HWE 刺激下,TNF-α 的分泌量从对照组的 7.82 ± 1.57 pg/mL 增加到 403.7 ± 59.7 pg/mL(p < 0.001)。值得注意的是,在Dectin-1基因敲除小鼠的骨髓树突状细胞中,C. militaris子实体热水提取物的这些免疫刺激作用被完全取消(所有细胞因子的p < 0.001),这表明Dectin-1对于C. militaris子实体热水提取物诱导的免疫调节是必不可少的。这些研究结果为我们提供了新的视角,使我们了解到小米草子实体热水提取物的免疫刺激作用机制,并凸显了其作为天然免疫调节剂在各种治疗应用中的潜力。
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来源期刊
CiteScore
2.60
自引率
16.70%
发文量
91
审稿时长
6-12 weeks
期刊介绍: The rapid growth of interest in medicinal mushrooms research is matched by the large number of disparate groups that currently publish in a wide range of publications. The International Journal of Medicinal Mushrooms is the one source of information that will draw together all aspects of this exciting and expanding field - a source that will keep you up to date with the latest issues and practice. The International Journal of Medicinal Mushrooms published original research articles and critical reviews on a broad range of subjects pertaining to medicinal mushrooms, including systematics, nomenclature, taxonomy, morphology, medicinal value, biotechnology, and much more.
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