补充药用虎乳菇 Lignosus rhinocerus TM02®(姬松茸)菌丝体可部分通过调节氧化应激缓解自发性高血压大鼠的高血压并减轻血管功能障碍

IF 1.4 4区 生物学 Q4 MYCOLOGY
Yi En Wong, Muhammad Fazril Mohamad Razif, Szu-Ting Ng, Chon-Seng Tan, Shin Yee Fung, Dharmani Murugan
{"title":"补充药用虎乳菇 Lignosus rhinocerus TM02®(姬松茸)菌丝体可部分通过调节氧化应激缓解自发性高血压大鼠的高血压并减轻血管功能障碍","authors":"Yi En Wong, Muhammad Fazril Mohamad Razif, Szu-Ting Ng, Chon-Seng Tan, Shin Yee Fung, Dharmani Murugan","doi":"10.1615/intjmedmushrooms.2024055061","DOIUrl":null,"url":null,"abstract":"Hypertension is a risk factor for cardiovascular diseases such as coronary artery disease, heart failure and stroke. Lignosus rhinocerus (Cooke) Ryvarden (also known as tiger milk mushroom) has been reported to exhibit a range of pharmacological effects, such as anti-inflammatory, anti-proliferative, antioxidative, immunomodulatory and anti-asthmatic activities. Thus far, limited research has explored its ability to mediate vascular effects in vivo. Therefore, this study investigated the antihypertensive and vascular protective effects of L. rhinocerus TM02® sclerotia supplementation in spontaneously hypertensive rats (SHR). Wistar Kyoto (WKY) rats served as a normotensive control group. SHR were orally administered with L. rhinocerus TM02® sclerotia (100 mg/kg and 300 mg/kg, respectively) for 8 weeks, and blood pressure was monitored every 2 weeks. Vascular function was evaluated using an organ bath (aorta) and wire myograph (renal artery) at the treatment endpoint. The levels of reactive oxygen species (ROS) and nitric oxide (NO) in the aorta and renal artery were evaluated using dihydroethidium (DHE) and difluoro fluorescein acetate (DAF-FM) fluorescence assays, respectively. Total plasma nitrate/nitrite and tumor necrosis factor alpha (TNF-α) levels were evaluated via colorimetric assays. In vivo treatment with L. rhinocerus TM02® sclerotia significantly attenuated the increase in systolic blood pressure (SBP). It also alleviated vascular dysfunction and decreased elevated ROS in the aorta and renal arteries of the treated SHRs. Moreover, L. rhinocerus TM02® sclerotia attenuated plasma TNF-α level but increased total plasma nitrate/nitrite, albeit slightly, coupled with significantly increased NO at the vascular level. Collectively, the present study demonstrated that L. rhinocerus TM02® sclerotia supplementation exerted blood pressure lowering effects, partly attributed to improvements in vascular function via reduction in vascular oxidative stress.","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Medicinal Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) Sclerotia Supplementation Mitigates Hypertension and Alleviates Vascular Dysfunction Partly Through Oxidative Stress Modulation in Spontaneously Hypertensive Rats\",\"authors\":\"Yi En Wong, Muhammad Fazril Mohamad Razif, Szu-Ting Ng, Chon-Seng Tan, Shin Yee Fung, Dharmani Murugan\",\"doi\":\"10.1615/intjmedmushrooms.2024055061\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Hypertension is a risk factor for cardiovascular diseases such as coronary artery disease, heart failure and stroke. Lignosus rhinocerus (Cooke) Ryvarden (also known as tiger milk mushroom) has been reported to exhibit a range of pharmacological effects, such as anti-inflammatory, anti-proliferative, antioxidative, immunomodulatory and anti-asthmatic activities. Thus far, limited research has explored its ability to mediate vascular effects in vivo. Therefore, this study investigated the antihypertensive and vascular protective effects of L. rhinocerus TM02® sclerotia supplementation in spontaneously hypertensive rats (SHR). Wistar Kyoto (WKY) rats served as a normotensive control group. SHR were orally administered with L. rhinocerus TM02® sclerotia (100 mg/kg and 300 mg/kg, respectively) for 8 weeks, and blood pressure was monitored every 2 weeks. Vascular function was evaluated using an organ bath (aorta) and wire myograph (renal artery) at the treatment endpoint. The levels of reactive oxygen species (ROS) and nitric oxide (NO) in the aorta and renal artery were evaluated using dihydroethidium (DHE) and difluoro fluorescein acetate (DAF-FM) fluorescence assays, respectively. Total plasma nitrate/nitrite and tumor necrosis factor alpha (TNF-α) levels were evaluated via colorimetric assays. In vivo treatment with L. rhinocerus TM02® sclerotia significantly attenuated the increase in systolic blood pressure (SBP). It also alleviated vascular dysfunction and decreased elevated ROS in the aorta and renal arteries of the treated SHRs. Moreover, L. rhinocerus TM02® sclerotia attenuated plasma TNF-α level but increased total plasma nitrate/nitrite, albeit slightly, coupled with significantly increased NO at the vascular level. Collectively, the present study demonstrated that L. rhinocerus TM02® sclerotia supplementation exerted blood pressure lowering effects, partly attributed to improvements in vascular function via reduction in vascular oxidative stress.\",\"PeriodicalId\":14025,\"journal\":{\"name\":\"International journal of medicinal mushrooms\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2024-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of medicinal mushrooms\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1615/intjmedmushrooms.2024055061\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of medicinal mushrooms","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1615/intjmedmushrooms.2024055061","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

高血压是冠心病、心力衰竭和中风等心血管疾病的危险因素。据报道,Lignosus rhinocerus (Cooke) Ryvarden(又称虎皮乳菇)具有一系列药理作用,如抗炎、抗增殖、抗氧化、免疫调节和抗哮喘。迄今为止,对其在体内介导血管效应能力的研究还很有限。因此,本研究对自发性高血压大鼠(SHR)补充犀角菌 TM02® 的降压和血管保护作用进行了调查。Wistar Kyoto(WKY)大鼠作为正常血压对照组。自发性高血压大鼠口服鼻炎灵 TM02® 硬菌(分别为 100 毫克/千克和 300 毫克/千克)8 周,每 2 周监测一次血压。在治疗终点,使用器官浴(主动脉)和钢丝肌电图(肾动脉)评估血管功能。主动脉和肾动脉中的活性氧(ROS)和一氧化氮(NO)水平分别通过二氢乙锭(DHE)和二氟醋酸荧光素(DAF-FM)荧光测定法进行评估。血浆硝酸盐/亚硝酸盐总量和肿瘤坏死因子α(TNF-α)水平通过比色法进行评估。用鼻炎灵 TM02® 硬菌进行体内治疗可显著降低收缩压(SBP)的升高。它还缓解了血管功能障碍,降低了接受治疗的 SHR 主动脉和肾动脉中升高的 ROS。此外,L. rhinocerus TM02® sclerotia 能降低血浆 TNF-α 水平,但能增加血浆硝酸盐/亚硝酸盐总量,尽管增加幅度较小,同时还能显著增加血管中的 NO。总之,本研究表明,补充鼻炎灵 TM02® 硬菌具有降低血压的作用,部分原因是通过降低血管氧化应激改善了血管功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Medicinal Tiger Milk Mushroom Lignosus rhinocerus TM02® (Agaricomycetes) Sclerotia Supplementation Mitigates Hypertension and Alleviates Vascular Dysfunction Partly Through Oxidative Stress Modulation in Spontaneously Hypertensive Rats
Hypertension is a risk factor for cardiovascular diseases such as coronary artery disease, heart failure and stroke. Lignosus rhinocerus (Cooke) Ryvarden (also known as tiger milk mushroom) has been reported to exhibit a range of pharmacological effects, such as anti-inflammatory, anti-proliferative, antioxidative, immunomodulatory and anti-asthmatic activities. Thus far, limited research has explored its ability to mediate vascular effects in vivo. Therefore, this study investigated the antihypertensive and vascular protective effects of L. rhinocerus TM02® sclerotia supplementation in spontaneously hypertensive rats (SHR). Wistar Kyoto (WKY) rats served as a normotensive control group. SHR were orally administered with L. rhinocerus TM02® sclerotia (100 mg/kg and 300 mg/kg, respectively) for 8 weeks, and blood pressure was monitored every 2 weeks. Vascular function was evaluated using an organ bath (aorta) and wire myograph (renal artery) at the treatment endpoint. The levels of reactive oxygen species (ROS) and nitric oxide (NO) in the aorta and renal artery were evaluated using dihydroethidium (DHE) and difluoro fluorescein acetate (DAF-FM) fluorescence assays, respectively. Total plasma nitrate/nitrite and tumor necrosis factor alpha (TNF-α) levels were evaluated via colorimetric assays. In vivo treatment with L. rhinocerus TM02® sclerotia significantly attenuated the increase in systolic blood pressure (SBP). It also alleviated vascular dysfunction and decreased elevated ROS in the aorta and renal arteries of the treated SHRs. Moreover, L. rhinocerus TM02® sclerotia attenuated plasma TNF-α level but increased total plasma nitrate/nitrite, albeit slightly, coupled with significantly increased NO at the vascular level. Collectively, the present study demonstrated that L. rhinocerus TM02® sclerotia supplementation exerted blood pressure lowering effects, partly attributed to improvements in vascular function via reduction in vascular oxidative stress.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
2.60
自引率
16.70%
发文量
91
审稿时长
6-12 weeks
期刊介绍: The rapid growth of interest in medicinal mushrooms research is matched by the large number of disparate groups that currently publish in a wide range of publications. The International Journal of Medicinal Mushrooms is the one source of information that will draw together all aspects of this exciting and expanding field - a source that will keep you up to date with the latest issues and practice. The International Journal of Medicinal Mushrooms published original research articles and critical reviews on a broad range of subjects pertaining to medicinal mushrooms, including systematics, nomenclature, taxonomy, morphology, medicinal value, biotechnology, and much more.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信