International journal of medicinal mushrooms最新文献_第10页

Sterols Content of Fruiting Bodies of Medicinal Artist's Bracket Mushroom Ganoderma applanatum (Agaricomycetes) Collected in Armenia. 亚美尼亚产药用菌灵芝子实体甾醇含量研究。

IF 1.2 4区生物学

International journal of medicinal mushrooms Pub Date : 2023-01-01 DOI: 10.1615/IntJMedMushrooms.2023048520

Susanna M Badalyan, Sergey S Hayrapetyan

{"title":"Sterols Content of Fruiting Bodies of Medicinal Artist's Bracket Mushroom Ganoderma applanatum (Agaricomycetes) Collected in Armenia.","authors":"Susanna M Badalyan, Sergey S Hayrapetyan","doi":"10.1615/IntJMedMushrooms.2023048520","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2023048520","url":null,"abstract":"The qualitative analysis of hexane extracts obtained from different trama layers (WT, T1-T4) of dried fruiting bodies of medicinal bracket fungus Ganoderma applanatum collected in the Tavoush region of North-East Armenia was performed by GC-MS analysis. Three sterols [(7.22-ergostadienon, ergosterol and ergosta-14.22-diene-3-ol (3β, 5α, 22E)] have been identified. The results have shown that the content and ratio of sterols differ in analyzed trama samples. The highest amount of sterols was detected in middle parts of T2 and T3 layers, while content of sterols gradually decreased to the upper cortical (T4) and lower hymenial (T1) layers. The chromatographic profiles of identified compounds indicate that different sterols dominated in each layer: 7.22-ergostadienon in T4, ergosterol in T3, T2, and T1. The average weight loss of analyzed trama samples during six days of drying was about 40 wt.% (37.0-43.49 wt.%) of the total weight of basidiome, which decreased up to 5 wt.% in the next two days. The complete extraction of sterols lasted six days. Its further prolongation leads to stationary phase without an increase in the amount of extracted sterols.","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":"25 7","pages":"65-74"},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10010646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of the Extraction Solvents on Dissolution Rate and Antioxidant Capacity of Auricularia auricula (Agaricomycetes) Polysaccharides In Vitro and In Vivo. 提取溶剂对木耳多糖体外和体内溶出率及抗氧化能力的影响

IF 1.2 4区生物学

International journal of medicinal mushrooms Pub Date : 2023-01-01 DOI: 10.1615/IntJMedMushrooms.2023048033

Jing Yao, Jiang-Ying Zeng, Yu-Xuan Tang, Yi-Rui Cheng, Tian-Yuan Li, Jun Yang, Yong-Jun Zhang

{"title":"Effects of the Extraction Solvents on Dissolution Rate and Antioxidant Capacity of Auricularia auricula (Agaricomycetes) Polysaccharides In Vitro and In Vivo.","authors":"Jing Yao, Jiang-Ying Zeng, Yu-Xuan Tang, Yi-Rui Cheng, Tian-Yuan Li, Jun Yang, Yong-Jun Zhang","doi":"10.1615/IntJMedMushrooms.2023048033","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2023048033","url":null,"abstract":"This paper reports the effects of solvents on the dissolution rate and antioxidant capacity of Auricularia auricula polysaccharides (AAPs). The ultra-low temperature combined with microwave extraction (UME) was used to compare the dissolution rates and molecular weights of AAPs using deionized water and deep eutectic solvents (DES) as solvents, respectively. Scanning electron microscope (SEM) was used to observe the effects of water extract (AAPs-FW) and DES extract (AAPs-FD) on the cell wall of A. auricula. The antioxidant capacity of polysaccharide extracts in vitro was assessed by using various methods (DPPH, ABTS, and hydroxyl radicals). In addition, in vivo oxidative stress was assessed using Caenorhabditis elegans models. The extract yield of AAPs varied among the extracts and was 19.58% ± 0.56% in AAPs-FW. Whereas DES-UME increased the yield of polysaccharides (AAPs-FD) by 9.81% in the extraction medium containing triethylene glycol-choline chloride, under the optimum conditions of 60 min freezing time, 350 W, and 90 s microwave time. The microstructure of the cell wall shown by SEM was consistent with the results of polysaccharide yields. The molecular weights of AAPs-FW and AAPs-FD were found to be 398.107 kDa and 89.099 kDa, respectively. The results demonstrated that AAPs-FD exhibited potent radical scavenging activity against DPPH and a weaker scavenging ability for ABTS and OH radicals compared to AAPs-FW. In addition, both polysaccharide extracts increased the survival rate of C. elegans under methyl viologen induced oxidative stress at specific concentrations (p < 0.05), and the antioxidant capacity of AAPs-FD was higher than that of AAPs-FW at low concentrations (0.125 mg/mL). This indicated that both polysaccharides had a protective effect against damage induced by intracellular free radical generators (methyl viologen).","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":"25 5","pages":"61-74"},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9474447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review on Edible Fungi-Derived Bioactive Peptides: Preparation, Purification and Bioactivities. 食用菌源生物活性肽的制备、纯化及生物活性研究进展。

IF 1.2 4区生物学

International journal of medicinal mushrooms Pub Date : 2023-01-01 DOI: 10.1615/IntJMedMushrooms.2023048464

Tiantian Zhou, Qingwei Li, Ming Zhao, Yu Pan, Xianghui Kong

引用次数: 0

The Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) Can Combat Cytokine Storm and Other COVID-19 Related Pathologies: A Review. 灵芝或灵芝药菇灵芝(菌丝菌)可以对抗细胞因子风暴和其他COVID-19相关病理:综述

IF 1.2 4区生物学

International journal of medicinal mushrooms Pub Date : 2023-01-01 DOI: 10.1615/IntJMedMushrooms.2023048109

Adekunle Babajide Rowaiye, Akwoba Joseph Ogugua, Doofan Bur, Timipanipiri Wood, Zainab Labbo, Chimaobi Chukwu, Femi Johnson Afolabi, Ezinne Janefrances Nwonu, Tarimoboere Agbalalah

{"title":"The Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) Can Combat Cytokine Storm and Other COVID-19 Related Pathologies: A Review.","authors":"Adekunle Babajide Rowaiye, Akwoba Joseph Ogugua, Doofan Bur, Timipanipiri Wood, Zainab Labbo, Chimaobi Chukwu, Femi Johnson Afolabi, Ezinne Janefrances Nwonu, Tarimoboere Agbalalah","doi":"10.1615/IntJMedMushrooms.2023048109","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2023048109","url":null,"abstract":"Coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 is characterized by acute respiratory distress syndrome (ARDS) facilitated by cytokine storm and other risk factors that increase susceptibility and complications leading to death. Emerging as a major global public health challenge, the disease has claimed more than 6 million lives and caused catastrophic global economic disruptions. However, there are concerns about the safety as well as the efficacy of drugs and vaccines presently used to control the pandemic, therefore necessitating intense global search for safe natural products that can effectively and safely combat it. This work reviews studies on lingzhi or reishi medicinal mushroom, Ganoderma lucidum and its properties that may potentially combat SARS-CoV-2 infection and the co-morbidities. Available evidence suggests that medicinal properties of the Ganoderma mushroom can combat the complications of SARS-CoV-2 infection and the co-morbidities that can aggravate the severity of the disease. Preclinical and clinical evaluation to establish dose, efficacy, and potential toxicity and possible use in the management of COVID-19 is recommended.","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":"25 5","pages":"1-15"},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9474444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Triterpene Yield from Ganoderma atrum HBSD Z19 (Agaricomycetes) by Optimization of Medium Components and Evaluation of Bioactivity Under Simulated Gastrointestinal Digestion In Vitro. 通过培养基成分优化及体外模拟胃肠道消化生物活性评价优化灵芝HBSD Z19 (agaricomyetes)三萜产率

IF 1.2 4区生物学

International journal of medicinal mushrooms Pub Date : 2023-01-01 DOI: 10.1615/IntJMedMushrooms.2023048024

Ruoyun Dong, Jie Qiao, Shiqin Song, Wanwan Zhuansun, Yiping Hu, Xin Zeng, Binyue Xin, Qimeng Weng, Huawei Zeng, Xian Yin

{"title":"Optimization of Triterpene Yield from Ganoderma atrum HBSD Z19 (Agaricomycetes) by Optimization of Medium Components and Evaluation of Bioactivity Under Simulated Gastrointestinal Digestion In Vitro.","authors":"Ruoyun Dong, Jie Qiao, Shiqin Song, Wanwan Zhuansun, Yiping Hu, Xin Zeng, Binyue Xin, Qimeng Weng, Huawei Zeng, Xian Yin","doi":"10.1615/IntJMedMushrooms.2023048024","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2023048024","url":null,"abstract":"The intracellular triterpene yield from Ganoderma atrum was enhanced by optimization based on single-factor experiments, Plackett-Burman experimental design (PBED) and response surface methodology (RSM) under liquid fermentation conditions. The optimal medium composition (g·L-1) was glucose (46.0), bean cake powder (30.2), KH2PO4 (2.0), CaCl2 (3.0), MgSO4 (1.5), FeSO4 (0.2), and pH 6.0. Under the optimal conditions, the highest triterpene yield of 0.527 g·L-1 was obtained, which was 4.705-fold higher than before optimization. The fermented powder that was collected from the optimal medium was subjected to simulated gastrointestinal digestion, with differences resulting from extraction in different digestive juices (purified water, simulated gastric digestive juice, simulated gastrointestinal digestive juice). The content of triterpenes and polysaccharides increased, except for total phenol content. In terms of the antioxidant activity, the 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH+⋅) scavenging activity gradually decreased whereas the 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+⋅) scavenging activity first decreased and then increased. In terms of enzyme viability, the activity of α-amylase (α-AL) and α-glucosidase (α-GC) in the digestive juices decreased dramatically. The main bioactive components of G. atrum and their bioactivity in digestive juices were evaluated, providing a reference for the effective use of fermented power from G. atrum.","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":"25 5","pages":"75-90"},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9474449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial, Antioxidant, Cytotoxicity and DNA Protective Properties of the Pink Oyster Mushroom, Pleurotus djamor (Agaricomycetes). 粉红色平菇的抗菌、抗氧化、细胞毒性和DNA保护特性

IF 1.2 4区生物学

International journal of medicinal mushrooms Pub Date : 2023-01-01 DOI: 10.1615/IntJMedMushrooms.2022046706

Şule Inci, Mehmet Akyüz, Sevda Kirbag

{"title":"Antimicrobial, Antioxidant, Cytotoxicity and DNA Protective Properties of the Pink Oyster Mushroom, Pleurotus djamor (Agaricomycetes).","authors":"Şule Inci, Mehmet Akyüz, Sevda Kirbag","doi":"10.1615/IntJMedMushrooms.2022046706","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2022046706","url":null,"abstract":"In this study, pink oyster mushroom Pleurotus djamor was cultivated using wheat straw (WS), quinoa stalk (QS), and their mixtures (WS-QS (1:1)) as substrate and evaluated in terms of antimicrobial, antioxidant, cytotoxicity, and DNA protective effects. Gram-positive and Gram-negative pathogen bacteria (Bacillus subtilis, Proteus vulgaris, Streptococcus mutans, Salmonella typhi, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli), dermatophyte (Trichophyton sp.) and yeast (Candida tropicalis) were used in the study. It was found to be very active against all bacteria (except S. mutans and S. typhi), and dermatophyte when compared to the control groups (8.7-33.3 mm), but low against C. tropicalis. It was seen that the best total antioxidant assay (TAS) value was 2.05 mmol/L on WS-QS (1:1). Depend on, it was determined that the total oxidant assay (TOS) value (5.26 μmol/L) in the same compost was lower than the others, and also the scavenging effect of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) was higher on WS at 25 mg/mL (84.20%). The methanol extract on WS at a concentration of 400 μg/mL, significantly reduced the percentage of viability in the human breast cancer (MDA-MB-231) cell line (2.2%). The methanol extracts on WS and QS medium were found to inhibit DNA damage induced by UV radiation and H2O2 at a concentration of 25 mg/mL. These results showed that pink oyster mushroom has benefits such as antimicrobial, antioxidant, cytotoxic, and DNA protective effects.","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":"25 2","pages":"55-66"},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10680752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fatty Acids Composition of Basidiocarps of Some Wood Associated Medicinal Mushrooms (Agaricomycetes) from India. 印度几种木生药用蘑菇(木生菌)担子果皮的脂肪酸组成。

IF 1.2 4区生物学

International journal of medicinal mushrooms Pub Date : 2023-01-01 DOI: 10.1615/IntJMedMushrooms.2022047269

Uzma Azeem, Richa Shri, Gurpaul Singh Dhingra

{"title":"Fatty Acids Composition of Basidiocarps of Some Wood Associated Medicinal Mushrooms (Agaricomycetes) from India.","authors":"Uzma Azeem, Richa Shri, Gurpaul Singh Dhingra","doi":"10.1615/IntJMedMushrooms.2022047269","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2022047269","url":null,"abstract":"In this investigation, crude fat contents and fatty acid compositions of lipids present in the basidiocarps of widely distributed, medicinally important, wild mushrooms (Fuscoporia torulosa, Inonotus pachyphloeus, Phellinus allardii, Ph. fastuosus, Ph. gilvus and Ph. sanfordii) collected from different localities of Dehradun, Uttarakhand, India were analyzed. Gas chromatography with flame ionization detector was performed to identify and quantify the individual fatty acids present in the lipids of each mushroom. Mushrooms exhibited comparable amounts of crude fats with maximum content (0.35%) in Ph. sanfordii. The dominant fatty acid in the examined mushrooms was palmitic acid (C16:0). Oleic acid (C18:1n9c) and linoleic acid (C18:2n6c) exhibited maximum contents among the monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs), respectively. Saturated fatty acids (SFAs) in F. torulosa, I. pachyphloeus and Ph. fastuosus were at higher concentrations than unsaturated fatty acids (UFAs). Ph. allardii, Ph. gilvus and Ph. sanfordii exhibited greater amounts of UFAs compared with SFAs. Among UFAs, MUFAs dominated the polyunsaturated ones except for I. pachyphloeus and Ph. sanfordii. Of the polyunsaturated fatty acids (PUFAs), the contents of ω6 PUFAs were higher than ω3 PUFAs except for Ph. gilvus. Interestingly, a single trans fatty acid, elaidic acid (C18:1n-9t) (0.54-2.34%) was noticed in F. torulosa, Ph. fastuosus and Ph. sanfordii only. The examined mushrooms also differed in UFAs/SFAs, MUFAs/SFAs, PUFAs/SFAs, ∑ω6/∑ω3 and (linoleic acid) C18:2n6c/(oleic acid) C18:1n9c ratios. The presence of essential and non-essential fatty acids may make the examined mushrooms befitting candidates for use in nutraceuticals and pharmaceuticals.","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":"25 3","pages":"87-97"},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9313897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of Potential Health Benefits of Insoluble Dietary Fiber from the Fruiting Body of Medicinal Mushroom Schizophyllum commune (Agaricomycetes) through Superfine Pulverization. 超细粉碎法增强药用蘑菇裂叶菌子实体中不溶性膳食纤维的潜在保健价值。

IF 1.2 4区生物学

International journal of medicinal mushrooms Pub Date : 2022-01-01 DOI: 10.1615/IntJMedMushrooms.2022045183

Bin Du, Caihong Cheng, Xuejun Ren, Yuedong Yang, Baojun Xu

{"title":"Enhancement of Potential Health Benefits of Insoluble Dietary Fiber from the Fruiting Body of Medicinal Mushroom Schizophyllum commune (Agaricomycetes) through Superfine Pulverization.","authors":"Bin Du, Caihong Cheng, Xuejun Ren, Yuedong Yang, Baojun Xu","doi":"10.1615/IntJMedMushrooms.2022045183","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2022045183","url":null,"abstract":"The objective of the current study is to reveal the influence of particle size of ground Schizophyllum commune and its extracted dietary fiber (DF) on physicochemical and antioxidant properties. Sch. commune powder and the extracted DF was ground by regular and superfine grinding, and their particle sizes were determined using laser diffraction particle size analyzer. The results indicated that superfine grinding could significantly pulverize DF particles to micro-scale; the particle size distribution presented a Gaussian distribution. The soluble DF in Sch. commune was increased effectively with superfine grinding. Sub-micronized insoluble DF showed increased total phenolic content (TPC) and ferric reducing antioxidant power (FRAP). Moreover, with particle size reduction, the oil binding capacity (OBC), nitrite ion absorption capacity (NIAC), cation-exchange capacity (CEC), cholesterol absorption capacity (CAC), and Pb ion adsorption capacity were significantly (p < 0.05) increased and the water retention capacity (WRC), swelling capacity (SC) and Cu, Zn ions adsorption capacity had no significant changes. A kind of health beneficial DF with higher soluble DF content, OBC, NIAC, CEC, CAC, Pb ion adsorption capacity and antioxidant activity (TPC and FRAP) was obtained through superfine grinding. Sch. commune DF could be potentially used as an ingredient for functional food and nutraceuticals.","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":"24 11","pages":"73-82"},"PeriodicalIF":1.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40463841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidative and Cytoprotective Effects of Ganoderma applanatum and Fomitopsis pinicola in PC12 Adrenal Phaeochromocytoma Cells. 平顶灵芝和细绒拟南芥对PC12肾上腺嗜铬细胞瘤细胞的抗氧化和细胞保护作用。

IF 1.2 4区生物学

International journal of medicinal mushrooms Pub Date : 2022-01-01 DOI: 10.1615/IntJMedMushrooms.2022045050

Xiu-Yue Li, Shu-Yao Li, Feng Yin, Hai-Min Chen, Dong-Feng Yang, Xiang-Qian Liu, Qing-Hao Jin, Xiao-Man Lv, Dennis Mans, Xiao-Dan Zhang, Zong-Suo Liang

{"title":"Antioxidative and Cytoprotective Effects of Ganoderma applanatum and Fomitopsis pinicola in PC12 Adrenal Phaeochromocytoma Cells.","authors":"Xiu-Yue Li, Shu-Yao Li, Feng Yin, Hai-Min Chen, Dong-Feng Yang, Xiang-Qian Liu, Qing-Hao Jin, Xiao-Man Lv, Dennis Mans, Xiao-Dan Zhang, Zong-Suo Liang","doi":"10.1615/IntJMedMushrooms.2022045050","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2022045050","url":null,"abstract":"Considering the impact of oxidative stress on the development of many diseases, together with the role of natural antioxidants in maintaining physiological balance in humans, medicinal mushrooms are potential sources of bioactive compounds against many diseases. In the present work, in vitro evaluation of the biological activities of the alcoholic extracts of two wild tree mushrooms, namely, Ganoderma applanatum and Fomitopsis pinicola, has been performed. Extraction of G. applanatum (GAE) and F. pinicola (FPE) was conducted with 60% ethanol and 100% ethanol sequentially. UPLC-MS/MS identification was conducted on the two mushrooms extracts. A total of 15 substances were identified in GAE, including 3 spiro meroterpenoids and 12 triterpenoids; a total of 14 chemical constituents were iden¬tified in FPE, including 8 triterpenoids, 4 triterpene glycosides, 1 lanosterol, and 1 lanostanoid. The resulting extracts were examined for their in vitro antioxidative and cytoprotective effects against AAPH-induced oxidative damage. Our results demonstrated that both extracts have potent antioxidative activities, when GAE was 0.2 mg/mL, the clearance rates of DPPH and ABTS have reached 93.34% and 99.93%, respectively. When FPE was 1.4 mg/mL and 0.6 mg/mL, the scavenging rates of DPPH and ABTS have reached 91.76% and 100%, respectively. Both the alcoholic extracts of G. applanatum and F. pinicola were able to protect the AAPH-induced damage and could effectively inhibit cell aging via β-galactosidase (SA β-gal) staining activity test and scanning electron microscopy analysis.","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":"24 10","pages":"15-29"},"PeriodicalIF":1.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40466499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Pulsed Light Mutagenesis of the Willow Bracket Mushroom, Phellinus igniarius (Agaricomycetes), for Enhanced Production of Flavonoids, Laccase, and Fermentation Biomass. 利用脉冲光诱变柳条菇(Phellinus igniarius)提高黄酮、漆酶和发酵生物量的研究。

IF 1.2 4区生物学

International journal of medicinal mushrooms Pub Date : 2022-01-01 DOI: 10.1615/IntJMedMushrooms.2022044961

Yating Dong, Haile Ma, Moses Kwaku Golly, Wenjuan Qu, Bei Wang, Cunshan Zhou, Xiaofei Ye, Henan Zhang, Bingcheng Gan, Junjie Yan, Xing Han, Jin Zhao, Rencai Feng, Renyun Miao, Zhiyuan Zhang

{"title":"Pulsed Light Mutagenesis of the Willow Bracket Mushroom, Phellinus igniarius (Agaricomycetes), for Enhanced Production of Flavonoids, Laccase, and Fermentation Biomass.","authors":"Yating Dong, Haile Ma, Moses Kwaku Golly, Wenjuan Qu, Bei Wang, Cunshan Zhou, Xiaofei Ye, Henan Zhang, Bingcheng Gan, Junjie Yan, Xing Han, Jin Zhao, Rencai Feng, Renyun Miao, Zhiyuan Zhang","doi":"10.1615/IntJMedMushrooms.2022044961","DOIUrl":"https://doi.org/10.1615/IntJMedMushrooms.2022044961","url":null,"abstract":"Phellinus igniarius is a medicinal fungus possessing potent therapeutic activity due to the polysaccharides, polyphenols, flavonoids, and other secondary metabolites they contain. Laccases are crucial enzymes involved in lignin degradation in Ph. igniarius and offer great potential to accomplish several bioprocesses. To generate Ph. igniarius strains with high biomass, flavonoid, and laccase activity, we used pulsed light (PL) technology for mutagenesis of Ph. igniarius protoplasts and screened for mutants with high biomass, flavonoid, and laccase activity. At the irradiation power of 100 J, treated distance 8.5 cm, irradiation frequency was 0.5 s/time, three times treatments, after five generations of selection, three mutants were obtained with higher biomass production. Compared with control, the mycelium biomass and the flavonoid production of the screened mutant strain QB72 were increased 20.87% and 53.51%, respectively. The total amount of the accumulated extracellular laccase of the QB72 in the first 6 and 8 days increased 23.38% and 22.37% respectively, and over the total 16 days it increased 9.62%. In addition, RAPD analysis results indicated that the genetic materials of the mutant QB72 were altered. PL mutagenesis method has great potential for developing strains, especially Phellinus.","PeriodicalId":14025,"journal":{"name":"International journal of medicinal mushrooms","volume":"24 10","pages":"31-43"},"PeriodicalIF":1.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40466500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1