{"title":"Carbocation scavenger enhanced pretreatment of wood at high solids loading – Effect on enzymatic conversion and fermentation","authors":"Matthias A.U. Eckl,Butrint Nikqi,Juri Salzmann,Johannes Ruhnke,Thomas Pielhop","doi":"10.1016/j.indcrop.2026.124467","DOIUrl":"https://doi.org/10.1016/j.indcrop.2026.124467","url":null,"abstract":"","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"53 1","pages":"124467"},"PeriodicalIF":5.9,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149151586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Miroslava Kačániová, Zhaojun Ban, Li Li, Jian Lou, Joel Horacio Elizondo-Luevano, Anis Ben Hsouna, Rania Ben Saad, Alessandro Bianchi, Stefania Garzoli
{"title":"Tea tree essential oil as a natural biocide: Chemical analysis and its efficacy against microbial, biofilm, and insect challenges","authors":"Miroslava Kačániová, Zhaojun Ban, Li Li, Jian Lou, Joel Horacio Elizondo-Luevano, Anis Ben Hsouna, Rania Ben Saad, Alessandro Bianchi, Stefania Garzoli","doi":"10.1016/j.indcrop.2026.124564","DOIUrl":"10.1016/j.indcrop.2026.124564","url":null,"abstract":"<div><div>Recent studies have demonstrated the potential of natural plant essential oils (EOs) as alternatives to chemical preservatives due to their notable antimicrobial activity, rapid biodegradability, and high safety profile. The aim of this study was to evaluate the chemical composition and biological activities of essential oil from <em>Melaleuca alternifolia</em> (tea tree). GC-MS analysis identified 31 volatile compounds, with γ-terpinene (25.3%) as the dominant component. Antioxidant activity was assessed using DPPH and ABTS assays, yielding IC₅₀ values of 28.07 mg/mL and 0.51 mg/mL, respectively. Antimicrobial efficacy was tested against twelve microorganisms using the disc diffusion method and determination of minimum inhibitory concentrations (MIC). The largest inhibition zones were observed against biofilm-forming <em>Salmonella enterica</em> (11.33 mm) and <em>S. enterica</em> (10.33 mm), with MIC<sub>50</sub> values ranging from 0.16 to 8.08 mg/mL. The highest <em>in situ</em> inhibition was recorded on the kiwi model against <em>Yersinia enterocolitica</em> (88.83%), on pear against <em>Staphylococcus aureus</em>, on parsley against <em>Listeria monocytogenes</em>, and on radish against biofilm-forming <em>S. enterica</em> all at the highest applied concentration of 500 µL/L. Anti-biofilm activity was evaluated using crystal violet staining and MALDI-TOF MS Biotyper spectral profiling. Spectral differences in MALDI-TOF MS profiles between planktonic and biofilm cells suggest that tea tree essential oil triggers distinct bacterial responses depending on physiological state and surface material. Insecticidal activity against adult <em>Megabruchidius dorsalis</em> yielded LC<sub>50</sub> and LC<sub>90</sub> values of 20.60% and 54.46%, respectively, with 100% mortality recorded at 100% essential oil concentration. These findings highlight the broad-spectrum bioactive potential of tea tree essential oil and its promise as a natural biocidal agent.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"252 ","pages":"Article 124564"},"PeriodicalIF":6.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149200459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Microbe-mediated regulation of cadmium partitioning and intra-plant detoxification in the medicinal plant Solanum aculeatissimum","authors":"Yanbo Yang, Qihang Cai, Zhenghai Sun, Liping Li","doi":"10.1016/j.indcrop.2026.124565","DOIUrl":"10.1016/j.indcrop.2026.124565","url":null,"abstract":"<div><div>Soil cadmium (Cd) pollution poses a serious threat to agricultural ecosystems, and plant-microbe interactions offer a promising strategy for Cd remediation. <em>Solanum aculeatissimum</em> is a medicinal and industrial plant with high Cd tolerance and accumulation potential, yet its interactions with associated microorganisms under Cd stress remain unclear. Metagenomic analyses revealed that Cd stress significantly reduced rhizosphere microbial diversity and promoted the enrichment of Cd-tolerant and potentially beneficial microorganisms. Notably, the abundance of <em>Achromobacter</em> increased markedly under high Cd treatment. Two core <em>Achromobacter</em> strains, Hach1 and Hach3, were isolated from roots. Both strains exhibited multiple plant growth-promoting traits, tolerated 800 mg/L Cd²⁺, and achieved Cd removal rates exceeding 60% in liquid culture. Inoculation experiments demonstrated that Hach1 significantly increased root Cd content by 35.6% and alleviated Cd-induced oxidative damage primarily by enhancing POD and CAT activities. In contrast, Hach3 enhanced Cd translocation to stems by 61.4% and to leaves by 56.2%, while improving Cd tolerance through enhanced SOD activity and proline accumulation. At the transcriptional level, application of the two core strains differentially regulated the expression of heavy metal ATPase (HMA) gene family members, thereby influencing Cd spatial distribution within plants. This study systematically elucidates the mechanisms underlying the cooperative responses of <em>S. aculeatissimum</em> and <em>Achromobacter</em> to Cd stress, providing mechanistic insights into plant-microbe interactions associated with Cd tolerance and adaptation.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"252 ","pages":"Article 124565"},"PeriodicalIF":6.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149200460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Changping Zhang, Ziting Chen, Yixuan Lin, Xiaodong Li, Yuya Geng, Jialong Sun, Gaoquan Dong, Jie Yan
{"title":"TkCPT1 modulates rubber particle size and natural rubber accumulation in Taraxacum kok-saghyz","authors":"Changping Zhang, Ziting Chen, Yixuan Lin, Xiaodong Li, Yuya Geng, Jialong Sun, Gaoquan Dong, Jie Yan","doi":"10.1016/j.indcrop.2026.124505","DOIUrl":"10.1016/j.indcrop.2026.124505","url":null,"abstract":"<div><div>Jasmonic acid (JA) signaling plays a pivotal role in regulating root architecture and natural rubber (NR) biosynthesis in <em>Taraxacum kok-saghyz</em> (<em>T. kok-saghyz</em>), yet its mechanistic connection with cis-prenyltransferase (CPT)-mediated rubber chain elongation remains largely unknown. In this study, eight TkCPT family members were identified at the whole-genome level. Among them, TkCPT1-TkCPT4 were differentially and markedly regulated by methyl jasmonate (MeJA), localized to the endoplasmic reticulum (ER), and possessed promoters enriched with JA-responsive cis-acting elements. Overexpression of <em>TkCPT1</em>, <em>TkCPT2</em>, and <em>TkCPT4</em> significantly promoted primary root elongation, whereas overexpression of <em>TkCPT3</em> had no significant effect on primary root length. All overexpression lines exhibited increased root biomass and enhanced NR content, with the OE-<em>TkCPT1</em> lines showing the greatest increase in rubber accumulation. Yeast two-hybrid and bimolecular fluorescence complementation assays demonstrated that TkCPT1-TkCPT4 physically interacted with TkCPTL1. Transmission electron microscopy and particle size distribution analyses further revealed a marked increase in rubber particle density in the OE-<em>TkCPT1</em> lines, accompanied by a shift in the predominant particle diameter from approximately 400 nm to 200 nm, resulting in a more homogeneous population of smaller rubber particles. Collectively, these findings demonstrate that TkCPT1 positively regulates natural rubber biosynthesis and rubber particle biogenesis, providing a promising molecular target for the genetic improvement of high-yielding <em>T. kok-saghyz</em> germplasm.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"252 ","pages":"Article 124505"},"PeriodicalIF":6.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149090511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Multi-omics reveals microbial community associations with metabolite accumulation in Gastrodia elata","authors":"Jian Chen, Hong-Gao Liu, Shun-Qiang Yang, Piao Chang, Yuan Yuan, Yu-Cheng Dai","doi":"10.1016/j.indcrop.2026.124477","DOIUrl":"10.1016/j.indcrop.2026.124477","url":null,"abstract":"<div><div><em>Gastrodia elata</em> is a medicinal herb widely cultivated in East Asia for treating headache and dizziness, and its efficacy largely depends on gastrodin, 4-hydroxybenzyl alcohol, and parishins. However, the basis for quality variation of <em>G. elata</em> across growth systems remains largely undetermined. Here, tubers and rhizosphere soils were collected from wild, wild‑simulated cultivated (WSC), and greenhouse cultivated (GHC) <em>G. elata</em>, and metabolite, transcript, and microbiome data were generated for comprehensive multi-omics analyses. We found that (1) wild <em>G. elata</em> accumulated significantly higher gastrodin and parishin C levels than GHC <em>G. elata</em>, and were enriched in organic acids and glycosides, whereas cultivated <em>G. elata</em> accumulated more sugars and amino acids. (2) gene expression patterns differed relatively little between wild and WSC <em>G. elata</em>, whereas GHC <em>G. elata</em> exhibited a distinct transcriptomic profile associated with defense activation. (3) wild and WSC <em>G. elata</em> harbored higher absolute microbial abundance (16S and ITS gene copy numbers) in the rhizosphere and were enriched in a diverse array of plant growth-promoting microorganisms. (4) microbial communities were associated with the metabolic and transcriptional divergence among growth systems. Soil properties and the rhizosphere bacterial community, particularly taxa such as <em>Burkholderia-Caballeronia-Paraburkholderia</em> and <em>Roseiarcus</em>, were closely associated with the accumulation of gastrodin and parishins. Notably, the absolute abundance of rhizosphere microbes was significantly correlated with gastrodin and parishins accumulation. In summary, this study offers a multi‑omics view of microbe–plant–metabolite associations in <em>G. elata</em>, providing valuable guidance for the rational design of synthetic microbial inoculants and future cultivation practices.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"252 ","pages":"Article 124477"},"PeriodicalIF":6.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149090517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Niche-differentiated assembly of the tobacco microbiome under pathogen invasion of black shank disease and the potential protective effects of an isolated Bacillus strain","authors":"Sujie Wang, Huabing Liu, Junjia Guo, Zechao Qu, Mengxiao Xu, Mengmeng Kong, Shizhou Yu, Jie Zhang, Jiemeng Tao, Huan Su, Qiao Wang, Jingjing Jin, Zefeng Li, Zhijun Tong, Peijian Cao, Linggai Cao, Peng Lu","doi":"10.1016/j.indcrop.2026.124549","DOIUrl":"10.1016/j.indcrop.2026.124549","url":null,"abstract":"<div><div>The assembly of plant-associated microbiomes along the root-stem continuum can contribute to a critical biological barrier against soil-borne diseases, yet the systemic dynamics of this assembly under pathogen invasion remain poorly understood. Here, we integrated 16S rRNA/ITS amplicon sequencing, culturomics, and dual-transcriptomic analysis to decipher the microbiome dynamics of tobacco under <em>Phytophthora nicotianae</em> (<em>P. nicotianae</em>) infection and elucidate the protective properties of a specific microbial member. Our results revealed that niche heterogeneity was the primary driver of microbial community assembly, with diversity progressively declining from the rhizosphere to the stem endosphere. Pathogen invasion was associated with altered spatial organization of the microbiome, characterized by compartment-dependent changes in bacterial-fungal co-occurrence networks. Under persistent pathogen pressure, asymptomatic tobacco plants maintained high relative abundance of <em>Bacillus</em> in stem endosphere, higher than in all root niches. Guided by this ecological cue, we isolated <em>Bacillus</em> sp. SX48, which significantly suppressed tobacco black shank in vivo. Transcriptomic profiling suggested that SX48 perturbed pathogen signaling and membrane/cell-wall-related processes and was accompanied by repression of biosynthetic pathways and induction of glycolysis-associated genes. In the host, SX48 enhanced plant growth and may modulate hormone-related pathways while strengthening cell wall-associated defenses, a response that may help limit pathogen spread from roots to stems. Collectively, these findings reveal compartment niche-specific microbiome dysbiosis during tobacco black shank disease and provide a microbiome-informed basis for developing <em>Bacillus</em>-based biocontrol strategies.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"252 ","pages":"Article 124549"},"PeriodicalIF":6.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149142106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chunpu Chen,Chunping Li,Kaimeng Bo,Yuting Liu,Changming Wang
{"title":"Funneliformis mosseae inoculation is associated with drought-intensity-dependent anatomical, physiological, and metabolic responses in Chimonobambusa quadrangularis","authors":"Chunpu Chen,Chunping Li,Kaimeng Bo,Yuting Liu,Changming Wang","doi":"10.1016/j.indcrop.2026.124523","DOIUrl":"https://doi.org/10.1016/j.indcrop.2026.124523","url":null,"abstract":"","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"45 1","pages":"124523"},"PeriodicalIF":5.9,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149151596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}