Industrial Crops and Products最新文献

{"title":"Curcumin derivative intercalated expandable graphite: Imparting flame retardancy, anti-dripping and smoke suppression for polylactic acid composites","authors":"Wei Tan ,&nbsp;Yin Tian ,&nbsp;Chunlong Zuo ,&nbsp;Yongli Zhang ,&nbsp;Xiaoyu Liu ,&nbsp;Ziyin Ye ,&nbsp;Lu Bai ,&nbsp;Yuanlin Ren ,&nbsp;Xiaohui Liu","doi":"10.1016/j.indcrop.2024.120305","DOIUrl":"10.1016/j.indcrop.2024.120305","url":null,"abstract":"<div><div>With the depletion of petrochemical resources and the escalating severity of white pollution, replacing petrochemical resources with bio-based resources aligns with sustainable development strategy. In this work, a novel biomass flame retardant (PCDAC) derived from curcumin was intercalated into expandable graphite (EG) to construct an intrinsically intumescent flame retardant system (EG@PCDAC), which was then melt blended with polylactic acid (PLA) to fabricate flame retardant, anti-dripping and smoke suppressive PLA composite (PLA/EG@PCDAC). The residual char of PLA/EG@PCDAC was 14.27 wt% under N₂ atmosphere, while reaching UL-94 V–0 rating. Furthermore, the peak heat release rate and total heat release of PLA/EG@PCDAC were reduced by 46.95 % and 19.02 % compared with original PLA, and the total smoke production and peak smoke production rate also decreased by 20.74 % and 72.09 %, respectively. This work offered green and feasible inspiration for exploring biomass flame retardants and renewable PLA-based functional materials.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"224 ","pages":"Article 120305"},"PeriodicalIF":5.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142841191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation and assessment of equilibrium solubility, Hansen solubility parameters and modeling of natural plant medicine artemisinin (Form Ⅰ) in three binary solvents at different temperatures","authors":"Pengshuai Zhang ,&nbsp;Binbin Wu ,&nbsp;Han Sun ,&nbsp;Haiyan Luo ,&nbsp;Ranran Feng ,&nbsp;Chunxia Jin ,&nbsp;Jixiu Deng ,&nbsp;Jiaxuan Xu ,&nbsp;Peng Li ,&nbsp;Shuoye Yang","doi":"10.1016/j.indcrop.2024.120313","DOIUrl":"10.1016/j.indcrop.2024.120313","url":null,"abstract":"<div><div>Artemisinin has been widely used in various countries around the world for the treatment of malaria, which has saved so many lives that it continues to attract the attention of scientific researchers. However, its clinical efficacy and potential applications in other fields could be affected by its poor water-solubility. Thus, it is necessary to study the solubility and crystallization of artemisinin. At the present study, the solubility of artemisinin (Form Ⅰ) in three (tetrahydrofuran + isopropanol, 1,4-dioxane + isopropanol, 1,4-dioxane + <em>n</em>-propanol) binary solvents were measured from 283.15 K to 323.15 K by a gravimetric method. The Ma model, Ideal model, Jouyban-van′t Hoff model and GSM model were selected to fit the obtained solubility. Furthermore, <em>ARD</em>, <em>RMSD</em> and <em>RD</em> were used to evaluate the correlate reliability of the thermodynamic models. The average 100<em>ARD</em> and 10⁴<em>RMSD</em> of the four models were 2.19 (Ma), 1.48 (GSM), 2.18 (Jouyban-van′t Hoff), 1.53 (Ideal) and 12.37 (Ma), 8.27 (GSM), 11.18 (Jouyban-van′t Hoff), 5.74 (Ideal), respectively. Additionally, DSC, TGA, PXRD and crystal morphology characterization analysis were used to analyze the crystal polymorphic change during the artemisinin dissolution process. Besides, Hansen solubility parameters and thermodynamic properties including Δ_sol<em>H</em>°, Δ_sol<em>S</em>°, Δ_sol<em>G</em>°, ζ_H and ζ_TS of artemisinin (Form Ⅰ) in the selected solvents were investigated. The research could give important guiding significance and make a fundamental contribution to enrich and expand the solubility database and thermodynamic mechanism of dissolution and crystallization process of artemisinin (Form Ⅰ).</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"224 ","pages":"Article 120313"},"PeriodicalIF":5.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142832855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hierarchical characteristics in radial direction of Bambusa textilis McClure and their influence on tensile properties","authors":"Xiaolong Hao ,&nbsp;Yanhui Wei ,&nbsp;Kaitai Yao ,&nbsp;Yuan Chen ,&nbsp;Haiyang Zhou ,&nbsp;Rongxian Ou ,&nbsp;Qingwen Wang","doi":"10.1016/j.indcrop.2024.120376","DOIUrl":"10.1016/j.indcrop.2024.120376","url":null,"abstract":"<div><div>Bamboo exhibits a unique hierarchical structure, allowing it to adjust its resistance to external load in natural conditions. However, there is a lack of basic data on the structure and performance of <em>Bambusa textilis</em> McClure (BTM). This work elucidates the structure and properties of BTM from macroscopic to ultra-microscopic and explored the factors potentially influencing the tensile properties of BTM. The results show that BTM exhibits significant gradient changes in its fiber volume ratio (<em>V</em>_f), chemical components, fiber morphology, microfibril angle, crystallite sizes, crystallinity, creep resistance, and tensile properties, while the mechanical properties of its fiber cell wall are relatively constant along the radial direction of the BTM culm. The tensile failure of BTM layers commonly forms a “broom shape”, which is affected by the distribution and morphology of the fiber bundles, parenchyma, and vessels. The promising tensile strength and modulus in the outer culm were 489.05 MPa and 37.12 GPa, respectively. Statistical analysis showed that the change in macroscopic structure exerts significant effect on tensile properties of BTM relative to the micro-and ultra-microscopic features. BTM contains 95 % monoclinic crystals with lattice constants approximating those of other higher plants. The rule of mixture was invalid in this material.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"224 ","pages":"Article 120376"},"PeriodicalIF":5.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142888004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"YOLO-TBD: Tea Bud Detection with Triple-Branch Attention Mechanism and Self-Correction Group Convolution","authors":"Zhongyuan Liu ,&nbsp;Li Zhuo ,&nbsp;Chunwang Dong ,&nbsp;Jiafeng Li","doi":"10.1016/j.indcrop.2025.120607","DOIUrl":"10.1016/j.indcrop.2025.120607","url":null,"abstract":"<div><div>Automatic Tea Bud Detection (TBD) is one of the core technologies in intelligent tea-picking systems Since the tea buds are small, dense, highly overlapped, and their colors are close to the background, accurate tea bud detection faces great challenges. In this paper, a tea bud detection method, named as YOLO-TBD, is proposed, which adopts YOLOv8 as the basic framework. Firstly, the Path Aggregation Feature Pyramid Network (PAFPN) in YOLOv8 is improved by incorporating the features from the 2nd layer into the PAFPN network. This modification enables better utilization of low-level features, such as texture and color information, thereby enhancing the network’s feature representation ability. Secondly, a Triple-Branch Attention Mechanism (TBAM) is designed and integrated into the output of the backbone network and the C2f module. This attention mechanism strengthens the features of the tea bud objects and suppresses background noise through feature channel interactions, without increasing the model parameters. Finally, a Self-Correction Group Convolution (SCGC) is proposed, which replaces the conventional convolution in the C2f module. This convolution establishes long-range spatial and channel dependencies around each spatial position, enabling a larger receptive field and better contextual information capture with fewer parameters, thereby mitigating false detections and missed detections of tea bud objects. The proposed modules are integrated into the YOLOv8 network architecture, resulting in the construction of three detection models with different parameters, namely YOLO-TBD-L, YOLO-TBD-M and YOLO-TBD-S, respectively. Experimental results on our self-built tea bud detection dataset and the publicly available GWHD_2021 dataset demonstrate that, compared with current methods, the proposed YOLO-TBD-L method can attain a state-of-the-art accuracy, with mAP value reaching 87.04 % and 94.5 %, respectively. And the proposed YOLO-TBD-S model achieves comparable detection accuracy to the YOLOv8-L model with much lower model parameters and computational complexity.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"226 ","pages":"Article 120607"},"PeriodicalIF":5.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green extraction of valuable compounds from the byproduct of oil extraction from forage radish seed","authors":"Natália Stevanato ,&nbsp;Jaqueline Hoscheid ,&nbsp;Ana Paula Peron ,&nbsp;Érica Marusa Pergo Coelho ,&nbsp;Camila da Silva ,&nbsp;Edson Antônio da Silva","doi":"10.1016/j.indcrop.2024.120257","DOIUrl":"10.1016/j.indcrop.2024.120257","url":null,"abstract":"<div><div>This work aimed to investigate the extraction of compounds from the byproduct of oil extraction (BOE) of forage radish seeds, using hydroethanolic solvent under pressurized liquid conditions. For this purpose, the influence of the variables temperature (100, 125 and 150 °C), pressure (50, 75 and 100 bar) and time (30 and 60 min) on the extraction process was evaluated. The chemical characterization, antibacterial activity, cytotoxicity and genotoxicity of the extracts was determined, as well as its purification. Temperature was the variable that most influenced the responses, resulting in greater overall yield (in terms of mass and active compounds) as it increased. A diverse phenolic profile was found in the extracts, encompassing different classes such as phenolic acids, flavonoids and aldehydes. The predominant compounds were sinapic, malic, nicotinic acids and rutin. The extract showed better antimicrobial activity against <em>Streptococcus pyogenes</em> with MIC value of 11.71 mg mL⁻¹. The concentrations of extracts evaluated were not cytotoxic or genotoxic to tissue with high cell proliferation. After purification with XAD 7HP resin, the content of phenolic compounds and the antioxidant capacity had a pronounced increase in the obtained product.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"224 ","pages":"Article 120257"},"PeriodicalIF":5.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142832854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-synergistic flame-retardant hydrogel coating for bamboo veneers","authors":"Haomeng Yu ,&nbsp;Zewei Ye ,&nbsp;Zhihao Yue,&nbsp;Shitao Shi,&nbsp;Yuanyuan Wang,&nbsp;Jiaqi Liao,&nbsp;Wenwen Zhu,&nbsp;Chencong Liu,&nbsp;Bo Chen,&nbsp;Qingfeng Sun,&nbsp;Xiaoping Shen","doi":"10.1016/j.indcrop.2024.120324","DOIUrl":"10.1016/j.indcrop.2024.120324","url":null,"abstract":"<div><div>The urgent demand for fire-safe coatings in bamboo furniture has driven significant progress in developing halogen-free flame-retardant technologies. This study presents a new multi-synergistic flame-retardant hydrogel coating, composed of methylcellulose (MC), polyacrylamide (PAAm), and tetraethyl orthosilicate (TEOS)-pretreated ammonium polyphosphate (SiO₂@APP) for bamboo veneers. The uniformly dispersed SiO₂@APP serves as an acid catalyst and an incombustible gas source, while MC and PAAm act as carbon sources in the fire-resistant C-Si-P-N coating system. Additionally, the hydrogel containing MC and water provides significant heat absorption, improving its flame-retardant efficacy. The limiting oxygen index (LOI) of the resultant hydrogel-coated bamboo (HB) increases significantly from 22.1 % to 55.9 %. Notable reductions of 20.3 % and 53.1 % in the peak heat release rate (PHRR) and total heat release (THR) of the hydrogel-coated bamboo (HB) are observed, respectively, in comparison to the original bamboo. After combustion, the dense hybrid char layer incorporating P, N, and Si effectively impedes heat release and combustible gas diffusion. Additionally, the hydrogel coating exhibits excellent interfacial adhesive strength (125.9 kPa) on the bamboo surface. Through this simple and facile sol-gel preparation process, efficient flame-retardant coatings demonstrate promising potential for biomass-based materials in diverse applications, including furniture, commercial buildings, and industrial uses.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"224 ","pages":"Article 120324"},"PeriodicalIF":5.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142832857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epoxidized safflower oil: Synthesis and evaluation of its performance as bioplasticizer for polylactic acid films","authors":"N.R. Amrutha ,&nbsp;P S Keshava Murthy ,&nbsp;Jeevan Prasad Reddy","doi":"10.1016/j.indcrop.2024.120360","DOIUrl":"10.1016/j.indcrop.2024.120360","url":null,"abstract":"<div><div>There is an emerging trend towards the utilization of biobased polymer formulation for massive applications like food packaging due to environmental concerns. Polylactic acid (PLA), a significant biopolymer, has faced limitations in industrial applications due to its brittleness and poor ductility. To overcome these drawbacks, bio-friendly oils have been employed as plasticizers, to ameliorate overall attributes and expand their use as a potential flexible packaging material. In this work, Safflower oil (SFO) was successfully epoxidized (ESFO) using an in-situ Prilezhaev reaction. ¹ H NMR and FTIR analysis indicated that the optimal conditions were a temperature of 70 °C and a residence time of 30 min, resulting in a remarkable maximum selectivity of 96 %. Further, SFO and ESFO modified PLA films were obtained by melt blending and cast film extrusion. The effect of oils on structural, mechanical and thermal properties of PLA was examined. Morphological analysis revealed a smoother surface along with improved thermal stability, moisture barrier, and hydrophobicity for PLA-ESFO (5 phr) films. Additionally, tensile properties emphasized the significant improvement in ductile properties for both the oils, particularly marked in 5 phr ESFO-based films, where 21 folds improved than neat PLA without compromising tensile strength and modulus. Therefore, epoxidized safflower oil exerted plasticization, encouraging the likelihood of PLA as a biodegradable packaging material.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"224 ","pages":"Article 120360"},"PeriodicalIF":5.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142884084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stability assessments of coloration and functionalization of cationic cotton fabric using flavonoid-rich extracts from Zanthoxylum bungeanum pericarps","authors":"Huiyu Jiang ,&nbsp;Wei Zeng ,&nbsp;Hongwen Mei ,&nbsp;Wen Zhang ,&nbsp;Xing–Xiang Ji","doi":"10.1016/j.indcrop.2024.120398","DOIUrl":"10.1016/j.indcrop.2024.120398","url":null,"abstract":"<div><div>This study investigates the use of flavonoid-rich extracts from <em>Zanthoxylum bungeanum</em> pericarps (ZBPFs) for sustainable dyeing and functionalization of cationized cotton fabrics. The extracts were obtained through ethanol/water extraction and purified using macroporous resin, with flavonoids such as rutin, astragalin, coumarin, baicalin, and quercetin making up over 72 % of the total composition. Stability tests revealed that ZBPFs were stable under neutral or mildly acidic conditions but were highly sensitive to reducing agents, prolonged light exposure, and metal ions. Cotton fabrics modified with 3-chloro-2-hydroxypropyl trimethylammonium chloride and dyed with ZBPFs reached a maximum color depth (K/S value) of 12.5 at 5 % owf. Fabrics dyed with 2 % owf ZBPFs demonstrated excellent UV protection (UPF of 115.54), antioxidant activity (98.1 % radical scavenging), and antibacterial efficacy (over 98 % against <em>E</em>. <em>coli</em> and <em>S</em>. <em>aureus</em>). The adsorption of ZBPFs onto cationized cotton fitted both the pseudo-second-order and Sips models. This indicates that chemical adsorption is the predominant mechanism and suggests multiple interactions between the ZBPFs and the fabric. The dyed fabrics exhibited high stability in rubbing and washing fastness, although their light fastness was comparatively lower. Further, washing with potassium aluminum sulfate significantly diminished the antioxidant and antibacterial properties, while washing with other solutions, such as tap water and deionized water, showed minimal effects on performance. This research highlights the potential of ZBPFs as a stable, bioactive, and environmentally friendly dye, offering considerable functional and ecological benefits in textile applications.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"224 ","pages":"Article 120398"},"PeriodicalIF":5.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142905123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kinetics, reaction mechanism and product distribution of lignocellulosic biomass pyrolysis using triple-parallel reaction model, combined kinetics, Py-GC/MS, and artificial neural networks","authors":"Chaowei Ma ,&nbsp;Yong Yu ,&nbsp;Cheng Tan ,&nbsp;Jianhang Hu ,&nbsp;Hua Wang","doi":"10.1016/j.indcrop.2024.120308","DOIUrl":"10.1016/j.indcrop.2024.120308","url":null,"abstract":"<div><div>Pyrolysis of biomass is a crucial process for the production of renewable energy and a sustainable alternative to fossil fuels. The present study analyzed the pyrolysis process and the composition of the products of six representative lignocellulosic biomasses using simultaneous thermal analyzer, pyrolysis - gas chromatography/mass spectrometry (Py-GC/MS), liquid-phase nuclear magnetic resonance (NMR) spectroscopy and X-ray photoelectron spectrometer (XPS). Furthermore, based on the TG results, the kinetics of the pyrolysis of three components of the six biomasses were explored using kinetic modelling, triple-parallel reaction model and Asym2sig deconvolution function. Subsequently, kinetic models of the six biomasses were developed using a combinatorial kinetic method. Finally, an artificial neural network (ANN) model was developed to predict the pyrolysis behaviors of the studied biomasses. For example, corn straw (CS) revealed three primary pyrolysis stages: below 400 K (volatilization of small molecules), 400–670 K (decomposition of major components), and above 670 K (charring of the residual components and secondary decomposition of intermediates). The optimum kinetics models for CS components were: <em>f</em>_<em>1</em><em>(α</em>_<em>1</em><em>) = α</em>_<em>1</em>^{<em>−0.80697</em>} <em>(1 − α</em>_<em>1</em><em>)</em>^{<em>1.99689</em>} <em>[−ln(1 − α</em>_<em>1</em><em>)]</em>^{<em>1.15425</em>}<em>, f</em>_<em>2</em><em>(α</em>_<em>2</em><em>) = α</em>_<em>2</em>^{<em>0.43522</em>} <em>(1 − α</em>_<em>2</em><em>)</em>^{<em>1.29066</em>} <em>[−ln(1 − α</em>_<em>2</em><em>)]</em>^{<em>0.32644</em>}<em>,</em> and <em>f</em>_<em>3</em><em>(α</em>_<em>3</em><em>) = α</em>_<em>3</em>^{<em>−2.82644</em>} <em>(1 − α</em>_<em>3</em><em>)</em>^<em>3</em> <em>[–ln(1 − α</em>_<em>3</em><em>)]</em>^{<em>−1.87480</em>}. Moreover, ANN23 showed the highest <em>R</em>² value (0.99908). Therefore, ANN23 is the most suitable model for predicting the pyrolysis of CS. The present research provides valuable references for the pyrolysis of biomass.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"224 ","pages":"Article 120308"},"PeriodicalIF":5.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142825730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan-mediated elicitation of secondary metabolism in Rhazya stricta and the in-silico exploration of phytochemicals as potential drug candidates against H1299-NSLC cell lines","authors":"Muhammad Uzair Javed ,&nbsp;Tehreem Mahmood ,&nbsp;Urooj Ali Khan ,&nbsp;Zain Asif Alvi ,&nbsp;Gouhar Zaman ,&nbsp;Razia Khurshid ,&nbsp;Nathalie Giglioli-Guivarc’h ,&nbsp;Caroline Birer Williams ,&nbsp;Vincent Courdavault ,&nbsp;Bilal Haider Abbasi","doi":"10.1016/j.indcrop.2024.120180","DOIUrl":"10.1016/j.indcrop.2024.120180","url":null,"abstract":"<div><div><em>Rhazya stricta</em> is a medicinally important plant known for its diverse pharmaceutical benefits, particularly in biosynthesizing anticancer and anti-inflammatory metabolites. For the eco-friendly and sustainable synthesis of phytochemicals and plant biomass, a biotechnological technique, ‘elicitation,’ is highly effective in various in vitro cultures. This study investigated the use of different concentrations of chitosan as a biotic elicitor in callus cultures of <em>R. stricta</em>, aiming to enhance biomass production, biological activities, and secondary metabolism. The findings demonstrated that maximum biomass production (FW: 40.91 ± 1.65 g/L; DW: 3.53 ± 0.11 g/L) was observed at 5 mg/L of chitosan as compared to the control. Secondary metabolites showed immense production [Phenolic content (14.50 ± 0.14 µg/mg; Flavonoid content (17.73 ± 0.80 µg/mg)] in callus cultures treated with 5 mg/L of chitosan as compared with control. Additionally, at a chitosan concentration of 1 mg/L, the elicited callus cultures had the maximum antioxidant capacity as measured by a phosphomolybdenum-based assay and DPPH free radical scavenging assay, with values of 131.82 1.53 µg/mg TAC and 94.6 % of DPPH inhibition. Callus extracts treated with 2 μg/mL of chitosan for 15 days significantly decreased the viability of H1299 cells to 60.52 %, showcasing the ability of chitosan to promote the cytotoxicity of plant extracts. In contrast to the control, cultures with chitosan concentrations of 5 mg/L revealed a considerable accumulation of pharmacologically active components, with 14 out of 19 alkaloids being the most accumulated compounds, as determined by LC-HRMS/MS analyses. Among the identified compounds, Eburnamine emerges as the most viable drug candidate that can be further screened against non-small cell lung cancer. This study highlights the potential of chitosan in augmenting the secondary metabolism of <em>R. stricta</em> callus cultures and provides insight into the cytotoxic potential of extracted phytochemicals, which could enhance the anticancer potential of pharmaceuticals.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"224 ","pages":"Article 120180"},"PeriodicalIF":5.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142841187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}