Industrial Crops and Products最新文献

{"title":"The synergetic effect of ultrafine grinding and alkaline hydrogen peroxide pretreatment for the enzymatic saccharification of corn stover","authors":"Junbao Li, Yuke Han, Suan Shi, Weihua Xiao, Lujian Han","doi":"10.1016/j.indcrop.2024.120186","DOIUrl":"https://doi.org/10.1016/j.indcrop.2024.120186","url":null,"abstract":"Cellulose in lignocellulosic biomass is difficult to hydrolyze during enzymatic hydrolysis. To further simplify the pretreatment process in high solid loading on the premise of reducing the consumption of chemical reagent and energy, an effective one step mechanochemical (combines wet ultrafine grinding (WUG) with alkaline hydrogen peroxide (AHP)) composite pretreatment (CP) method for corn stover was developed. This new method could be performed at room temperature with low H₂O₂ dosages (0.05–0.15 g/g corn stover) and short processing time (0.5–0.1 h). The CP pretreatment removed lignin and acetyl groups, disrupted the cell structure of corn stover, effectively superimpose the enhancement effects of AHP and WUG. Its optimized enzymatic glucose yield was 254.23 g/kg with a low H₂O₂ dosage (0.10 g/g corn stover) and grinding time (0.5 h). The single pretreatment of WUG and AHP at the same condition could only achieve a glucose yield of 163.85 g/kg and 132.76 g/kg, respectively. The quantitative linear regression equation between cellulose content and enzymatic glucose yield (GY) for the CP pretreatment was found to be: GY= 31.78 × cellulose content - 979.40. The slope was higher than that for AHP pretreatment (23.26). This suggests that CP pretreatment with low H₂O₂ doses can improve the efficiency of enzymatic saccharification.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"1 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142789952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional wood-plastic composites: A review of research progress on flame retardancy, weather resistance and antimicrobial properties","authors":"Zihan Zhao, Zhihao Zhang, Hongxing Wang, Chunfeng Li, Lei Le, Mingli Liu","doi":"10.1016/j.indcrop.2024.120196","DOIUrl":"https://doi.org/10.1016/j.indcrop.2024.120196","url":null,"abstract":"Wood-plastic composites (WPC) have attracted a lot of academic attention due to their excellent mechanical properties and environmental friendliness. WPC is emerging as a sustainable and high-performing material consisting of wood fibers reinforced with a plastic matrix that has applications in a variety of industries, including construction. However, WPC still has some deficiencies, such as flame retardancy, aging resistance, and antimicrobial properties, which need to be further improved in some demanding industries. The wood fibers in WPC are flammable and have poor aging resistance, and the poor compatibility in the multiphase structure of WPC can lead to fungal or bacterial attack, which can degrade and ultimately damage its mechanical properties, so it is important to improve its flame retardancy, aging resistance, and antimicrobial properties to prolong its life. Therefore, it is crucial to improve its flame retardancy, aging resistance and antimicrobial properties to extend its outdoor service life. In this paper, the commonly used flame retardants, anti-aging agents and antimicrobial agents for WPC and their mechanisms of action are reviewed.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"4 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142789945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional role of calcium chloride in improving the chemical, mechanical, and physical properties of natural and synthetic rubber latex for gloves and transdermal patch films","authors":"Phataravarin Kantasa, Atiporn Obormkul, Nuchnapa Tangboriboon","doi":"10.1016/j.indcrop.2024.120183","DOIUrl":"https://doi.org/10.1016/j.indcrop.2024.120183","url":null,"abstract":"Synthetic and natural rubber latex gloves and transdermal patch films were prepared using a dipping process with unglazed porcelain molds. Calcium chloride (CaCl₂) was added to the rubber latex formulations at concentrations of 0, 0.2, 0.4, and 0.8 wt% to enhance their chemical, mechanical, and physical properties. The formation and characteristics of the rubber gloves and transdermal patch films were evaluated using standard testing methods. Calcium chloride, an inorganic compound composed of calcium ions (Ca^2 +) and chloride ions (Cl^-) with a pH of 2–3, proved highly effective in influencing the isoelectric point of the rubber latex compounds. It reduced the isoelectric point from −68.1 mV to −45 mV, maintaining it within the range conductive to stable film formation. Furthermore, it adjusted the pH of the rubber latex compounds from 10.5 to a range of 5.89–6.29 on the surface of the rubber film. Additionally, it facilitated ionic crosslinking between calcium ions and carboxylate groups on the particulate surface of natural rubber latex. These adjustments led to gradual improvements in tensile strength, elongation at break, and maximum stress of the dipped films, while simultaneously reducing water-extractable proteins. To further optimize the process, a small amount of Terric 16 A-16 was incorporated into the inorganic solution. This addition shortened coagulation time, particularly for synthetic rubber latex compound, and improved the physical properties of natural rubber latex. The resulting films were smooth, thin, and free of surface porosity. A key advantage of this study is the elimination of the need for a coagulant coating on the ceramic mold surface, achieved through a one-step dipping process. This innovation significantly reduces costs, processing time, and waste.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"9 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142788507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pinpointing MQTLs and candidate genes related to early maturity in upland cotton through the integration of meta‑analysis, RNA-seq, and VIGS approaches","authors":"Wenmin Yuan, Ying Li, Wenjiao Zhang, Jisheng Ju, Xuefeng Guo, Junning Yang, Hai Lin, Caixiang Wang, Qi Ma, Junji Su","doi":"10.1016/j.indcrop.2024.120195","DOIUrl":"https://doi.org/10.1016/j.indcrop.2024.120195","url":null,"abstract":"Early maturity is one of the primary traits associated with fiber yield, fiber quality and mechanized harvesting in China’s cotton agriculture. Mapping QTLs and mining candidate genes associated with maturity in cotton are critical for molecular improvement efforts that aim to increase early maturity traits in cotton. Integrating a large collection of cotton QTLs is an effective approach for revealing meta-QTLs (MQTLs) to identify candidate genes influencing early maturity in upland cotton. In this research, 1096 original QTLs linked to nine early maturity related traits were identified from 37 independent studies. Additionally, 772 initial QTLs were projected onto a consensus map containing 5051 markers, and 84 MQTLs were identified for traits related to early maturity. Among the MQTLs, 37 regions were verified via SNP loci reported in genome-wide association studies (GWAS) on early maturity traits in cotton. Finally, 118 candidate genes were successfully annotated in five major MQTL regions. Two candidate genes (<em>GhMYOB7</em> and <em>GhNSRB</em>) linked to early maturity were identified via RNA-seq and qRT<img alt=\"single bond\" src=\"https://sdfestaticassets-us-east-1.sciencedirectassets.com/shared-assets/55/entities/sbnd.gif\" style=\"vertical-align:middle\"/>PCR experiments. Furthermore, virus-induced gene silencing (VIGS) revealed that <em>GhMYOB7</em> and <em>GhNSRB</em> are crucial genes that control early flowering in cotton. The results of this study lay a foundation for exploring the genetic mechanisms underlying early maturity traits in upland cotton and provide a theoretical basis for the development of early maturity cotton cultivars.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"1 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142782787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing the enzymatic hydrolysis efficiency of wheat straw pulp residue by prewashing and ferric chloride assisted hydrothermal pretreatment","authors":"Kaijie Lu, Zhiwei Fan, Yue Liao, Xinxing Wu, Hongzheng Liu, Min Jin, Hui Wang, Shuaibo Han, Yan Zhang, Fangli Sun","doi":"10.1016/j.indcrop.2024.120238","DOIUrl":"https://doi.org/10.1016/j.indcrop.2024.120238","url":null,"abstract":"The presence of ash in lignocellulosic materials poses a challenge to achieving optimal conditions for autohydrolysis, thereby impeding efficient enzymatic conversion of biomass. This study investigates the synergistic effects of prewashing and the addition of ferric chloride (FeCl₃) to alleviate the adverse impact of both exogenous and endogenous ash on wheat straw pulp residue (WSPR) autohydrolysis. The findings indicated that as the water ratio (0–2000 w/v) increased and FeCl₃ (20 mM) was added, the removal of xylan and lignin from WSPR enhanced from 64.5 % to 92.4 % and from 20.7 % to 37.5 %, resulting in enhanced enzymatic efficiency of pretreated WSPR from 49.6 % to 90.7 %. The efficacy of these strategies was elucidated via analyses of cellulase accessibility, lignin surface area, particle size, and zeta potential. Furthermore, autohydrolysis of pretreated WSPR yielded a maximum xylooligosaccharide production rate of 171.7 mg/g WSPR. Consequently, the results demonstrated the effectiveness of prewash and FeCl₃ treatments in improving autohydrolysis efficiency and subsequent enzymatic hydrolysis of high ash biomass.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"20 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142788541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance of mild acetone organosolv fractionation on lignocellulosic feedstocks from new cropping systems for production of advanced bioethanol","authors":"Karla Dussan, Michiel Hoek, Truus de Vrije, Rick van de Vondervoort, Petra Bonouvrie, Rumeysa Caliskan, Andrea Parenti, Walter Zegada-Lizarazu, Andrea Monti, Arjan T. Smit, Ana M. López-Contreras","doi":"10.1016/j.indcrop.2024.120156","DOIUrl":"https://doi.org/10.1016/j.indcrop.2024.120156","url":null,"abstract":"Various lignocellulose feedstocks were sourced through an innovative double crop rotation system for production of advanced bioethanol. Dedicated biomass crops that grow with high yields with low cost and greenhouse gas emissions (hemp, biomass sorghum, and sunn hemp) were integrated with the cultivation of conventional food crops to recover agricultural residues (wheat straw, corn stover). These feedstocks with low indirect land use change risk were subjected to a biorefinery approach for the production of advanced ethanol from cellulosic and hemicellulosic sugars and co-production of lignin. The processing steps included tandem pre-extraction and acetone organosolv fractionation, followed by enzymatic hydrolysis of the obtained cellulose-enriched pulp (producing mainly C6 sugars), detoxification of hemicellulose hydrolysate (mainly C5 sugars) and fermentation of C5 and C6 sugars to ethanol. High recovery of cellulose in the pulp (87–100 %), C5 oligomeric and monomeric sugars in the hydrolysate (80–90 %) and isolated lignin (72–84 %) showed that process efficiency can be maintained across the various types of feedstocks. The ethanol production titres and productivity obtained by fermentation by <em>Saccharomyces cerevisiae</em> yeast on the C6 sugar enzymatic hydrolysates were robust and similar to those values obtained on model glucose substrate. C5 sugar hydrolysates after detoxification and evaporation were readily converted to ethanol with <em>Spathaspora passalidarum</em> CBS 10155. Sunn hemp and hemp feedstocks showed both less optimal enzymatic digestibility of cellulosic pulps and lower ethanol production rates from their hemicellulose hydrolysates. The present work shows the applicability of the aforementioned biorefinery approach for the production of advanced ethanol with a diverse set of lignocellulosic feedstocks obtained from an integrated food and bioenergy production system based on rotating crops. It also highlights that the most critical processing steps for process monitoring and optimisation are the pulp enzymatic hydrolysis of pulps and the detoxification of the C5 sugar hydrolysates.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"20 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142782790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and optimization of different extraction and analytical methods on Humulus lupulus L. with molecular networks, quantifications and desirability approaches","authors":"Valentin Pichon, Marion Millot, Emilie Pinault, Guillaume Hamion, Gilles Ducret, Marylène Viana, Lengo Mambu","doi":"10.1016/j.indcrop.2024.120166","DOIUrl":"https://doi.org/10.1016/j.indcrop.2024.120166","url":null,"abstract":"Specific natural compounds of <em>Humulus lupulus</em> L. (hop cones) have significant applications both in food and pharmaceutical industries. The effectiveness of these applications depends on the extraction methods used, which can vary in the yield and metabolite profile of the extract. This study focuses on the development of green solvent extraction methods in order to adapt the process to industrial-scale extraction in other plant species. Four extraction techniques were employed, including maceration (simple maceration (SM) and triphasic-SE), ultrasound (UAE), high pressure (PLE), microwaves (MAE). Two of them were also combined (UAE+SM). Ethanol and a combination of ethanol and water were mostly used. Triphasic extraction was performed with classical solvents. The results were compared in term of yield, total assays (polyphenols, flavonoids, and chlorophylls), metabolite profile, LC-MS/MS quantification, and molecular networking. UAE led to high yield with reduced extraction time compared to maceration, and this result was further amplified with pressure-assisted extraction (PLE) with a 3- and 6-fold increase in yield and time reduction, respectively. The yields were correlated with higher quantities of molecules of interest, such as α/β acids, polyphenols, and flavonoids. For instance, the MAE and PLE techniques provided high content of flavonoids and polyphenols (2 and 3-fold), xanthohumol (6 and 4-fold) compared to SE. The use of alternative extraction methods alone or coupled significantly improved the yields and contents. LC-MS/MS allowed for the monitoring of various metabolite concentrations, which were also found in molecular networks, and that facilitating the selection of the most appropriate technique. A descriptive statistical analysis was used to provide a global vision between the different extractive methods and determine the best experimental conditions. Among the various treatments tested, the global desirabilities from 65.5 % and 77.4 % were achieved with PLE followed by UAE-B+SM (64.5 %). They emerged as the most effective approach providing a balance between mass yields, content and concentration of metabolites of interest.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"20 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142782744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response surface methodology for supercritical CO2 extraction and microencapsulation of multi-components from “Pericarpium Citri Reticulatae - Fructus Aurantii” contained in herbal formulations","authors":"Xianghui Yao, Qing Cao, Gan Peng, Yaping Li, Min He","doi":"10.1016/j.indcrop.2024.120171","DOIUrl":"https://doi.org/10.1016/j.indcrop.2024.120171","url":null,"abstract":"To optimize the retention of the lipophilic compounds and aromatics, this study integrates supercritical CO₂ extraction (SCE) and microencapsulation technologies applied to \"<em>Pericarpium Citri Reticulatae</em> - <em>Fructus Aurantii</em>\" (PCR-FA). A three-factor, three-level experimental design employing response surface methodology (RSM) was conducted to assess the impact of pressure (P), temperature (T), and time (t) on the total extraction yield (Y) and the concentration of key constituents. The analysis of SCE products was performed using both sensory analysis and an electronic nose, followed by microencapsulation through complex coacervation. The findings revealed the presence of volatile terpenoids, lipophilic flavonoids, fatty acids, and sterols, with pressure exerting the significant influence on Y. Also extreme values of T and t adversely impacted the extraction efficiency. A sophisticated interaction among P, T, and t was demonstrated in the extraction of terpenoids, flavonoids, and lipids. An advanced adaptive chaotic grey wolf optimizer (ACGWO) pinpointed optimal conditions at 400 bar, 48 °C, and 87 minutes. The PCR-FA extracts exhibited distinctive olfactory traits and a unique electronic nose profile, differentiating them from individual herbal extracts. Post-microencapsulation, electron microscopy and ultraviolet (UV) spectroscopy confirmed a tri-layer structure encapsulating the SCE constituents. This investigation lays a scientific groundwork for the extraction and stabilization of active components from <em>Citrus</em>-based herbal combinations, offering substantial insights for the enhancement of Chinese patent medicine production through validated models and optimization algorithms.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"33 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142782786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the mechanism of straw-derived humic acid in mitigating urea nitrogen volatilization loss in four Chinese soils","authors":"Zeng Liu, Piao Zhu, Wenqi Han, Wenjie Su, Jianyuan Jing, Yingqiang Zhang, Qizhong Xiong, Xinxin Ye","doi":"10.1016/j.indcrop.2024.120188","DOIUrl":"https://doi.org/10.1016/j.indcrop.2024.120188","url":null,"abstract":"Natural humic acids can regulate urea conversion and mitigate urea nitrogen (N) volatilization losses. However, information regarding whether straw-derived artificial humic acid exhibits similar functionalities is unclear. Rice straw-derived artificial humic acid (A-HA) was synthesized via hydrothermal reaction, and A-HA-enhanced urea (A-HAU) was prepared. The impact of A-HAU on urea conversion and ammonia volatilization was investigated through soil incubation in four representative Chinese soils: calcareous meadow soil (CMS), black soil (BS), lime concretion black soil (LCBS), and red earth soil (RES). Multispectral characterization techniques were employed to elucidate the effect mechanism of A-HA on urea hydrolysis and ammonia volatilization. The A-HAU application significantly decreased ammonia volatilization from urea by 10.76 %, 30.64 %, 14.87 %, and 27.17 % in CMS, BS, LCBS, and RES soils, respectively, compared to conventional urea application. The mitigation of ammonia volatilization losses for CMS, BS, and LCBS soils was associated with the inhibition of urease activity and delayed urea hydrolysis; however, for RES soil, it was correlated with the attenuation of soil pH increase. The inhibitory effect of A-HAU on urease activity can be attributed to the ability of A-HA to react with the sulfhydryl groups of urease, resulting in reduced urease activity. Moreover, A-HA could react with urea during A-HAU production, which was also beneficial in retarding urea hydrolysis. Overall, A-HA for humic acid-enhanced urea production effectively mitigated urea-N volatilization losses, offering a novel approach to improve straw resource utilization and providing an additional option for urea synergist development.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"3 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142782788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and protein interactions of the TIFY family in the bioenergy plant Jatropha curcas L.","authors":"Yunliang Chen, Shanshan Lan, Xingyuan Liu, Yanfei Bai, Jin Sha, Zhurong Zou, Shuanglong Yang","doi":"10.1016/j.indcrop.2024.120194","DOIUrl":"https://doi.org/10.1016/j.indcrop.2024.120194","url":null,"abstract":"<em>Jatropha curcas</em> L. is an important energy crop whose growth is affected by abiotic stress. TIFY proteins regulate plant growth and respond to adverse conditions in higher plants. However, little information is available regarding the functions of the TIFY family genes in <em>J. curcas</em>. In this research, 14 members of the JcTIFY family were identified in <em>J. curcas</em> at the genome-wide level. These members belong to four subfamilies: jasmonate-ZIM-domain (JAZ), ZIM/ZIM-like, PEAPOD, and TIFY, and they are distributed across seven linkage groups. The TIFY proteins, consisting of 135–458 amino acids, are all hydrophilic and are predicted to be located in the nucleus. Although members of the same branch have different exon-intron structures, they share similar conserved domains and motif compositions. Protein interaction predictions indicate that TIFY proteins can form dimers and are involved in the jasmonic acid and ethylene signaling pathways. The role of TIFY in the growth of <em>J. curcas</em> was demonstrated by the analysis of cis-acting elements and expression of TIFY under various conditions and tissues. Furthermore, RT-qPCR analysis revealed that chilling stress and methyl jasmonate significantly induced the expression of JcJAZs, particularly JcTIFY5B. Yeast two-hybrid analysis revealed clear interactions between JcJAZs. Luciferase complementation and yeast two-hybrid assays demonstrated that JcTIFY10 formed a homodimer both <em>in vivo</em> and <em>in vitro</em>. JcJAZ proteins can form heterodimers with other proteins of the same family, and some proteins can form homodimers via the TIFY domain. The subcellular localization of JcTIFY5A and JcTIFY10 was in the nucleus, while JcTIFY9 was found in both the cytoplasm and nucleus. These results provide significant insights into the TIFY gene-mediated abiotic stress response and plant hormone signal transduction events in <em>J. curcas</em>.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"7 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}