Shuyu Luan, Suhaib Nisar, Jonathan Phipps, Antonio Ovejero-Pérez, Jason P. Hallett, Pedro Verdía Barbará
{"title":"Optimization of the ionoSolv process for the preservation of pulp fibre dimensions","authors":"Shuyu Luan, Suhaib Nisar, Jonathan Phipps, Antonio Ovejero-Pérez, Jason P. Hallett, Pedro Verdía Barbará","doi":"10.1016/j.indcrop.2025.121996","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.121996","url":null,"abstract":"The pulping industry, dominated by century-old technologies, is highly polluting, making imperative finding more environmentally friendly alternatives. The ionoSolv process, based on the use of low-cost protic ionic liquids, is one of the most promising methods to fractionate lignocellulose with low ecological impact. However, this process has been only optimized to produce sugar-derived chemicals, where fibre quality is not a key factor. Earlier studies have shown the detrimental effect of the standard ionoSolv conditions on fibre length, which renders them unsuitable for fibre applications, including the production of paper. For the first time, this study shows the optimization of the ionoSolv fractionation of <em>Miscanthus</em> to preserve key fibre properties of the pulps, namely fibre dimensions, degree of polymerization and relative molecular mass, while maintaining optimal purity. Different temperatures (150 ºC and 170 ºC), retention times (45–90 min), and acid:base ratios (a:b = 1.00 to a:b = 0.55) were investigated. Fractionation at 170 ºC for 45 min with a reduced acid:base ratio of a:b = 0.86 allowed for maximum delignification (lignin content of 4.4 %) and preservation of fibre length. Pulps recovered under optimal conditions were bleached using a single stage method based on H<sub>2</sub>O<sub>2</sub> increasing pulp purity, achieving optimal decolorization and retaining fibre dimensions comparable to those obtained from a commercial process (Lc(l) = 0.509 mm and Lc(w) = 0.775 vs Lc(l) = 0.540 mm and Lc(w) = 0.975). This highlights the potential of the ionoSolv process as a greener fractionation alternative to produce cellulose fibre materials.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"93 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jiarui Cui , Shubin Cao , Jie Hao , Yao Zhang , Shuang Gao , Jianshe Li , Fatimah Shuang Ma , Longguo Wu
{"title":"Construction of an industrial detection model for tomato organic acids based on low-rank adaptation and spatial feature fusion","authors":"Jiarui Cui , Shubin Cao , Jie Hao , Yao Zhang , Shuang Gao , Jianshe Li , Fatimah Shuang Ma , Longguo Wu","doi":"10.1016/j.indcrop.2025.121909","DOIUrl":"10.1016/j.indcrop.2025.121909","url":null,"abstract":"<div><div>Tomato (Solanum lycopersicum L.) is not only a globally important crop for human consumption but also a potential source of industrially valuable organic acids. Organic acids such as citric and malic acid have widespread applications in bio-based chemical synthesis, fermentation, biodegradable plastics, and green solvents. In this study, we develop a novel non-destructive framework combining Low-Rank Adaptation (LoRA) with Adaptive Spatial Feature Fusion Network (ASFFN) to accurately predict organic acid content using hyperspectral imaging (HSI). The model integrates advanced feature fusion strategies and robust outlier detection to improve generalizability across tomato varieties. Comparative experiments demonstrate the superior performance of the proposed LoRA-ASFFN over conventional CNN and PLSR baselines. The model enables rapid and precise identification of tomatoes with high organic acid concentrations, providing an efficient pathway for industrial processing and extraction. This research contributes to advancing bio-based chemical supply chains and improving the economic value of tomato crops through data-driven, precision screening techniques.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"236 ","pages":"Article 121909"},"PeriodicalIF":6.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Regulatory mechanism of anthocyanin biosynthesis in Dendrobium officinale : Integrating metabolome and transcriptome to reveal the potential application for quality improvement","authors":"Chenning Zhao, Jiaqi Ren, Yu Zhang, Qing Xu, Aimin Lv, Liang Wei, Qingsong Shao, Chenfei Lu","doi":"10.1016/j.indcrop.2025.121987","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.121987","url":null,"abstract":"The MYB and bHLH transcription factors could participate in the synthesis of anthocyanins through interaction or upstream-downstream regulation. Anthocyanins, as one of the most important active substances in plants, have high nutritional value of human beings. At present, anthocyanin biosynthesis has been widely studied, however, the regulatory mechanism of anthocyanins in <em>Dendrobium officinale</em> (<em>D. officinale</em>) remain unknow. Here, the anthocyanin components of two different colored cultivars from <em>D. officinale</em> were analyzed by UPLC-MS/MS. We have found that delphinidin and its derivatives were the main anthocyanin components in <em>D. officinale</em>, among which Malvidin-3-O-glucoside and Malvidin-3-O-galactoside were the main active substances. Subsequently, the key structural genes <em>DoCHS1/2</em>, <em>DoF3H</em>, <em>DoF3’H</em>, <em>DoDFR1/2</em>, <em>DoANS</em>, and transcription factors <em>DoMYB5–1</em>, <em>DoMYB7</em>, <em>DobHLH5–1</em> were identified based on transcriptomic analysis, which were mainly involved in the anthocyanin biosynthesis. Among that, both <em>DoMYB5–1</em> and <em>DoMYB7</em> could interact with <em>DobHLH5–1</em> to form complexes (<em>DoMYB5–1-DobHLH5–1</em>, <em>DoMYB7-DobHLH5–1</em>), and then regulated the expression of <em>DoDFR1</em> and <em>DoF3’H</em> to promote anthocyanin synthesis through the protein-protein interaction, transient overexpression experiments and Y1H assay. Furthermore, we also found that the interaction of <em>DoMYB5–1</em> and <em>DobHLH5–1</em> could cascade-rergulate the complex <em>DoMYB7-DobHLH5–1</em>, thereby regulating the expression of <em>DoDFR1</em> and then participating in the regulation of anthocyanin metabolism in <em>D. officinale</em>. Overall, this study clarified the regulatory mechanism of anthocyanin accumulation in <em>D. officinale</em>, which was helpful to reveal the biological mechanism of secondary metabolism in plants and provided a theoretical reference for the quality improvement of <em>D. officinale</em>.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"32 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145189389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Cinnamomum verum L. on intestinal glucose absorption and its use in the treatment of type 2 diabetes","authors":"Hamza Assaggaf","doi":"10.1016/j.indcrop.2025.122028","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.122028","url":null,"abstract":"<em>Cinnamomum verum</em> is an aromatic plant widely used in daily life. Scientific work has shown that cinnamon has hypoglycemic properties and may be very beneficial in controlling blood sugar levels in type 2 diabetes. In this work, we evaluated the inhibitory properties <em>Cinnamomum verum</em> aqueous extracts on intestinal glucose absorption and its antihyperglycemic effect <em>in vitro</em> using digestive functions enzymes, namely α-amylase and α-glucosidase, and we evaluated the subacute toxicity profiles of cinnamon extracts in rats through the approaches described in the guidelines of the OECD. The results show that the aqueous crude extract of cinnamon has no inhibitory effect on the second messenger, the Na+ /glucose cotransporter. Consequently, cinnamon has no effect on intestinal glucose absorption from an electrogenic point of view.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"18 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Upcycling agro-industrial biomass molasses into D-mannitol via an enzymatic cascade with in situ cofactor regeneration","authors":"Hyeonseon Bak, Jimin Park, Inchan Kwon","doi":"10.1016/j.indcrop.2025.122016","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.122016","url":null,"abstract":"Molasses, a sugar industry by-product rich in sucrose, D-glucose, and D-fructose, remains underutilized in high-value biomanufacturing. In this study, we present an efficient enzymatic cascade for the direct conversion of molasses into D-mannitol, a valuable sugar alcohol widely used in the food and pharmaceutical sectors. The cascade integrates three enzymes: invertase for sucrose hydrolysis, mannitol dehydrogenase (MDH) for D-fructose reduction, and glucose dehydrogenase (GDH) for in situ NADH regeneration via D-glucose oxidation, which simultaneously generates D-gluconolactone as a value-added coproduct. Two reaction formats were evaluated: a two-step system that sequentially optimizes enzyme-specific conditions, and a one-pot system enabling operational simplicity. The two-step method yielded 137 ± 13 mM D-mannitol, corresponding to ∼92 % conversion, while the one-pot system produced 123.1 ± 1.3 mM, corresponding to ∼95 % conversion under molasses conditions within 24 h. The molasses pretreatment was not essential for D-mannitol production. Enzymes maintained high catalytic activity in the complex molasses matrix, and glucose supplementation improved cofactor regeneration, eliminating residual D-fructose. This integrated strategy offers a sustainable and scalable platform for agro-industrial waste valorization by co-producing D-mannitol and D-gluconolactone, aligning with circular bioeconomy principles and advancing green bioprocessing technologies.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"28 3 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Andleeb Tajammal , Sammer Fatima , Huma Qureshi , Tauseef Anwar , Hossam S. El-Beltagi , Ibrokhim Ismoilov , Feruza Tukhtaboeva , Nazih Y. Rebouh , Maryam M. Alomran , Mohd Asif Shah
{"title":"Green-synthesized copper nanoparticles enhance drought tolerance in wheat for sustainable biomass and industrial applications","authors":"Andleeb Tajammal , Sammer Fatima , Huma Qureshi , Tauseef Anwar , Hossam S. El-Beltagi , Ibrokhim Ismoilov , Feruza Tukhtaboeva , Nazih Y. Rebouh , Maryam M. Alomran , Mohd Asif Shah","doi":"10.1016/j.indcrop.2025.121934","DOIUrl":"10.1016/j.indcrop.2025.121934","url":null,"abstract":"<div><div>Drought stress severely limits the growth, physiological performance, and yield of <em>Triticum aestivum</em> L. (wheat), particularly under water-limited conditions relevant to industrial and bioenergy applications. This study evaluated the potential of green-synthesized copper nanoparticles (CuNPs), produced using <em>Syzygium aromaticum</em> (L.) Merr. & L.M.Perry) (clove) extract, to enhance drought tolerance in four wheat varieties—Dharab-11, Watan-94, Faisalabad-2008, and Faisalabad-2010—selected based on contrasting stress sensitivity and germination performance. Copper nanoparticles with an average crystalline size of 23.6 nm were applied as foliar sprays at 10, 20, and 30 ppm, while drought stress was imposed at the grain-filling stage by withholding irrigation for 15 days, reducing soil moisture to approximately 40 % of field capacity. CuNP supplementation, particularly at 30 ppm, significantly improved growth, physiological, and biochemical traits under drought. Dharab-11 exhibited the tallest plants (87 cm), Watan-94 maintained the largest leaf area (52 cm²), and Watan-94 also produced the longest spikes (22.5 cm). Relative water content increased up to 94 %, while proline accumulation reached 0.78 µmol g⁻¹ fresh weight, reflecting enhanced osmotic adjustment. Total soluble sugars increased up to 0.96 µmol g⁻¹ fresh weight, supporting cellular turgor and metabolic stability under stress. Yield components, including spikelet number and 1,000-grain weight, were also positively influenced. These findings demonstrate that clove-extract-mediated CuNPs provide a novel, eco-friendly approach to mitigate drought-induced morphological, physiological, and biochemical impairments in wheat. Future field trials are recommended to validate scalability, assess nanoparticle persistence in soil, and evaluate potential phytotoxicity at higher concentrations.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"236 ","pages":"Article 121934"},"PeriodicalIF":6.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145189384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aminu Musa , Hajara Wada Bawa , Nura Suleiman Gwaram , Ibrahim Salisu , Tawfik A. Saleh , Muhammad Abdulkadir
{"title":"Sustainable green neem oil–based polymeric and Nanocrystalline cellulose additives: Synthesis, characterization, and performance in lubricating oils","authors":"Aminu Musa , Hajara Wada Bawa , Nura Suleiman Gwaram , Ibrahim Salisu , Tawfik A. Saleh , Muhammad Abdulkadir","doi":"10.1016/j.indcrop.2025.121956","DOIUrl":"10.1016/j.indcrop.2025.121956","url":null,"abstract":"<div><div>There has been significant interest in the use of green additives produced from bio-based materials within the lubricant industry. These additives are characterized by their non-toxic nature, cost-effectiveness, natural biodegradability, and diverse applications. The current study focuses on the synthesis of a Neem oil-derived homo-polymer, as well as a copolymer formed from Neem oil with Nanocrystalline cellulose (NCC). GC-MS analysis identified the fatty acid composition of neem oil, which was primarily dominated by the presence of oleic acid (∼19 %), which offers crucial reactive sites for the process of free-radical polymerization. This led to the formation of both homo-polymers and copolymers. Following this, FTIR confirmed the formation of both copolymer and homopolymer due to the absence of the olefinic peaks in the 1550–1680 cm<sup>−1</sup> region as opposed to their presence in the neat neem oil. The successful isolation of nanocrystalline cellulose (NCC) was validated through FTIR, which indicates a complete removal of the characteristic lignin peak around 1500–1600 cm⁻¹ and the hemicellulose carbonyl peak near 1735 cm⁻¹ , both of which are observable in the raw husk. The TGA analysis demonstrated the enhanced thermal stability of the copolymer, with about 5 % weight loss at 300°C in contrast to a 15 % loss for the homopolymer at the same temperature. The pour point and Viscosity Index of the base oil were analysed before and after the addition of the synthesized additives at different concentrations. Specifically, the polymers (both homo-polymer and copolymer) were evaluated at concentrations of 1 %, 2 %, and 3 % (w/v), whereas the NCC was assessed at 1 % and 2 % (w/v). The base oil containing 2 % copolymer was found to be optimal, yielding the highest Viscosity Index of 111.1 and a notably lower pour point of −14°C. These results confirmed the efficiency and applicability of synthesized NCC, a homo-polymer of neem oil, and copolymer as additives for lubricating oil to improve, specifically, its pour point and Viscosity Index.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"236 ","pages":"Article 121956"},"PeriodicalIF":6.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mengting Yu, Yujing Sun, Na Ji, Lei Dai, Man Li, Xuyan Dong, Liu Xiong, Qingjie Sun
{"title":"Superhydrophobic chitosan films via SiO2/PDMS coating for reducing liquid food residue","authors":"Mengting Yu, Yujing Sun, Na Ji, Lei Dai, Man Li, Xuyan Dong, Liu Xiong, Qingjie Sun","doi":"10.1016/j.indcrop.2025.122003","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.122003","url":null,"abstract":"Superhydrophobic coatings have gained significant interest due to their ability to prevent liquid foods like juice and yogurt from adhering to the inner surfaces of containers, thus reducing waste. Superhydrophobic coatings limit its application due to its toxicity, degradability and other reasons. In this work, chitosan sodium phytate films were deposited on glass plates by layer-by-layer self-assembly (LBL), then SiO<sub>2</sub> nanoparticles were introduced to construct micro/nano-structures, and finally, surface modification with polydimethylsiloxane (PDMS) was carried out to prepare superhydrophobic films. The surface of the superhydrophobic film had a micro/nano-scale structure similar to that of lotus leaves. The water contact angle (WCA) of superhydrophobic films reached 153°, and sliding contact angle (SA) was 5°. Owing to this unique structure, different liquid food droplets could roll off freely in a near spherical form on the chitosan phytate film surfaces, effectively reducing residue during container pouring. The outcomes of this study are expected to expand the potential applications of superhydrophobic chitosan films in liquid food anti-adhesion.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"93 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Guoping Shu, Yueli Tang, Mohammad Mahmoud Nagdy, Lingjiang Zeng, Fangyuan Zhang, Min Chen, Jingxin Mao, Zhihua Liao
{"title":"AabZlP1 positively regulates the biosynthesis of monoterpenes and sesquiterpenes in Artemisia annua","authors":"Guoping Shu, Yueli Tang, Mohammad Mahmoud Nagdy, Lingjiang Zeng, Fangyuan Zhang, Min Chen, Jingxin Mao, Zhihua Liao","doi":"10.1016/j.indcrop.2025.122024","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.122024","url":null,"abstract":"<em>Artemisia annua</em> synthesizes not only artemisinin but also diverse monoterpenes and non-artemisinin sesquiterpenes, which are widely utilized as aromatic and flavoring agents in the food, cosmetic, and healthcare industries. However, the regulatory mechanisms governing their biosynthesis remain unclear. In this study, we report that AabZIP1, an abscisic acid (ABA)-responsive bZIP transcription factor, modulates both monoterpene and sesquiterpene biosynthesis in <em>A. annua</em>. ABA exposure upregulated the expression of numerous terpene synthase (<em>TPS</em>) genes, including <em>AaBPS</em>, <em>AaTPS5</em>, <em>AaBFS</em>, <em>AaCPS</em>, <em>AaECS</em>, and <em>AaGAS</em>. Dual-luciferase assays, yeast one-hybrid, and electrophoretic mobility shift assays demonstrated that AabZIP1 directly bound to the promoters of <em>AaBPS</em>, <em>AaCPS</em>, and <em>AaGAS</em>, and indirectly modulates the others. Overexpression of <em>AabZIP1</em> led to the significant increase of <em>β</em>-pinene, camphene, <em>β</em>-caryophyllene, cedrol, and germacrene A contents in transgenic plants. These findings establish AabZIP1 as a positive modulator of terpene synthesis and offer a molecular target for engineering <em>A. annua</em> to enhance the production of high-value terpenoids<em>.</em>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"100 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zijie Zhang , Feiran Chen , Jie Fang , Huiyin Jin , Tongli Wang , Shengzuo Fang
{"title":"Metal and non-metal nanoparticles differentially regulate flavonoid accumulation in Cyclocarya paliurus: A multi-omics analysis","authors":"Zijie Zhang , Feiran Chen , Jie Fang , Huiyin Jin , Tongli Wang , Shengzuo Fang","doi":"10.1016/j.indcrop.2025.122023","DOIUrl":"10.1016/j.indcrop.2025.122023","url":null,"abstract":"<div><div>Nanotechnology offers emerging tools for elevating metabolites levels in agricultural and medicinal crops, yet its regulatory effects on woody species remains underexplored. <em>Cyclocarya paliurus</em>, a precious tree species with flavonoid-linked antioxidant and hypoglycemic properties, was foliar-applied with nanoparticles (NPs) of ZnO, MnO<sub>2</sub>, and SiO<sub>2</sub> to investigate their elicitation potential under field-simulated conditions. Compared with the control, applications of MnO<sub>2</sub>- and SiO<sub>2</sub>-NPs markedly increased total flavonoid content (TFC) in <em>C. paliurus</em> leaves by 21.26–49.99 %, and enhanced per-plant flavonoid accumulation by 40.70–50.06 %. Conversely, applications of high-dose ZnO-NPs induced oxidative imbalance, resulting in a 30.25 % and 43.84 % decline in TFC and flavonoid accumulation respectively. Multi-omics profiling revealed that both MnO<sub>2</sub>- NPs and SiO<sub>2</sub>-NPs activated ROS-dependent pathways involving MAPK and glutathione signaling, while SiO₂-NPs may also promote flavonoid biosynthesis via enhancing carbon fixation and primary metabolism. The MYB-bHLH-WD40 complex (including <em>MYB113</em>, <em>bHLH42</em>, and <em>TTG1</em>) was identified as a central regulatory node, ranking among the top 10 contributors in O2PLS analysis and exhibiting strong correlations (cor > 0.95) with flavonoid metabolites. Collectively, we propose a model wherein ROS signaling induced by NPs interacts with transcriptional networks to modulate flavonoid accumulation. This work provides a nano-enabled strategy to boost health-promoting compounds in the woody plants.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"236 ","pages":"Article 122023"},"PeriodicalIF":6.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145189331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}