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GhDLDH1 up-regulates synthesis of chloroplast responding to salinity stress in Gossypium hirsutum L.
IF 5.6 1区 农林科学
Industrial Crops and Products Pub Date : 2025-03-08 DOI: 10.1016/j.indcrop.2025.120823
Yi Liu , Yapeng Fan , Yuyuan Qian , Shuai Wang , Hui Huang , Xiugui Chen , Junjuan Wang , Lanjie Zhao , Lixue Guo , Yuping Sun , Zhining Yang , Xi Zhang , Shaoqi Li , Guangen Wang , Jianhong Zhang , Wuwei Ye
{"title":"GhDLDH1 up-regulates synthesis of chloroplast responding to salinity stress in Gossypium hirsutum L.","authors":"Yi Liu ,&nbsp;Yapeng Fan ,&nbsp;Yuyuan Qian ,&nbsp;Shuai Wang ,&nbsp;Hui Huang ,&nbsp;Xiugui Chen ,&nbsp;Junjuan Wang ,&nbsp;Lanjie Zhao ,&nbsp;Lixue Guo ,&nbsp;Yuping Sun ,&nbsp;Zhining Yang ,&nbsp;Xi Zhang ,&nbsp;Shaoqi Li ,&nbsp;Guangen Wang ,&nbsp;Jianhong Zhang ,&nbsp;Wuwei Ye","doi":"10.1016/j.indcrop.2025.120823","DOIUrl":"10.1016/j.indcrop.2025.120823","url":null,"abstract":"<div><div>D-lactate dehydrogenase (D-LDH) is a key enzyme in the pyruvate metabolic pathway and is crucial for plant growth, development, and stress tolerance. This study provides a systematic analysis of the genetic characteristics, evolutionary history, <em>cis</em>-acting elements, and expression patterns of the <em>D-LDH</em> gene family in cotton with 14 <em>D-LDH</em> genes were discovered. Phylogenetic analysis showed that <em>D-LDH</em> family genes could be clustered into 2 clades. The <em>D-LDH</em> genes of the four cotton species show a closer relationship to those of <em>T. cacao</em> compared to other plant species. Structure analysis showed that <em>GhDLDH</em> genes from the same subgroup exhibited similar genetic structure. The <em>D-LDH</em> family members predominantly include elements associated with hormones, the light response, the drought response, stress and the temperature response. <em>GhDLDH1</em> was significantly upregulated following exposure to various stress treatments. The quantitative real-time PCR (qRT-PCR) results demonstrated that most <em>GhDLDH</em> genes responded positively to salt stress, with <em>GhDLDH1</em> exhibited the peak transcriptional activity. Subcellular localization analysis revealed that GhDLDH1 is specifically localized within the chloroplast. Compared with those of pYL156 plants, pYL156: <em>GhDLDH1</em> plants presented significantly reductions in pyruvate (PA), lactic acid (LA), glutathione (GSH), lactic dehydrogenase (LDH), and chlorophyll levels, with a significant increase in the methylglyoxal (MG) content under salt stress. <em>GhDLDH1</em> gene silencing in cotton seedlings resulted in heightened sensitivity to NaCl stress, as evidenced by trypan blue staining. Plants in which the <em>GhDLDH1</em> gene was silenced exhibited lower chloroplast levels compared with pYL156 plants, and the structure was less coherent. Potential interactions were revealed between the D-LDH protein and the chloroplast-associated proteins PKP1 and PKP3. And the qRT<img>PCR data revealed altered expression levels of <em>GhPKP1</em> and <em>GhPKP3</em> following <em>GhDLDH1</em> gene silencing. In conjunction with the observed changes in chloroplast number and morphology, these findings suggest that <em>GhDLDH1</em> may be a key regulator of chloroplast function. This study serves as a valuable reference for investigating the interaction between the <em>D-LDH</em> gene and chloroplast-associated genes, as well as their regulatory effects on cotton salt tolerance.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"227 ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143575553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comprehensive analysis of safflower R2R3-MYBs reveals the regulation mechanism of CtMYB76 on flavonol biosynthesis
IF 5.6 1区 农林科学
Industrial Crops and Products Pub Date : 2025-03-07 DOI: 10.1016/j.indcrop.2025.120795
Zhengwei Tan , Dandan Lu , Lei Li , Xiaoyu Su , Yao Sun , Lina Wang , Yongliang Yu , Xiufu Wan , Lanjie Xu , Chunming Li , Huizhen Liang
{"title":"Comprehensive analysis of safflower R2R3-MYBs reveals the regulation mechanism of CtMYB76 on flavonol biosynthesis","authors":"Zhengwei Tan ,&nbsp;Dandan Lu ,&nbsp;Lei Li ,&nbsp;Xiaoyu Su ,&nbsp;Yao Sun ,&nbsp;Lina Wang ,&nbsp;Yongliang Yu ,&nbsp;Xiufu Wan ,&nbsp;Lanjie Xu ,&nbsp;Chunming Li ,&nbsp;Huizhen Liang","doi":"10.1016/j.indcrop.2025.120795","DOIUrl":"10.1016/j.indcrop.2025.120795","url":null,"abstract":"<div><div>The <strong>s</strong>afflower <strong>(</strong><em>Carthamus tinctorius</em><strong>)</strong> is an important plant used for medicine and food. R2R3-MYB transcription factors play vital roles in regulating plant biological processes. In this study, we identified 102 R2R3-MYBs in the <em>C. tinctorius</em> genome. Phylogenetic analysis indicated 36 combined clades, and one independent <em>Carthamus</em> clade. Flower color, tissue and developmental specific expression patterns of <em>R2R3-MYB</em> genes were observed in <em>C. tinctorius</em>. Almost all <em>R2R3-MYB</em>s contained some <em>cis</em>-acting elements that associated with abiotic and biotic responses. Twelve <em>R2R3-MYB</em> genes contained the LTR, MBS and MeJA elements were selected to test their expressional responses under drought, cold and MeJA conditions. Results show all these treatments induced large scale upregulation in most of these genes, with drought stress inducing the highest changes. Overexpression of <em>CtMYB76</em> in tobacco significantly induced the upregulation of flavonoid biosynthesis structural genes, accompanied by accumulation of flavonols. Dual-luciferase reporter and yeast one-hybridization analyses confirmed that CtMYB76 can bind with the promoter of structural genes and activate their expression. This study not only provided an in-depth understanding of the molecular basis of R2R3-MYB TFs in <strong>s</strong>afflower but also highlighted their potential applications in agriculture.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"227 ","pages":"Article 120795"},"PeriodicalIF":5.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hydrothermal carbonization of cola nut shell: Impact of deep eutectic pretreatment on the property of hydrochar
IF 5.6 1区 农林科学
Industrial Crops and Products Pub Date : 2025-03-07 DOI: 10.1016/j.indcrop.2025.120792
Félix Mérimé Bkangmo Kontchouo , Hao Xu , Marie Ganmo Koutchouo , Kuan Ding , Chayanoot Kositanont , Chotiros Dokkhan , Thipjak Na Lampang , Chanchana Thanachayanont , Xun Hu , Chaoyang Xu , Shu Zhang
{"title":"Hydrothermal carbonization of cola nut shell: Impact of deep eutectic pretreatment on the property of hydrochar","authors":"Félix Mérimé Bkangmo Kontchouo ,&nbsp;Hao Xu ,&nbsp;Marie Ganmo Koutchouo ,&nbsp;Kuan Ding ,&nbsp;Chayanoot Kositanont ,&nbsp;Chotiros Dokkhan ,&nbsp;Thipjak Na Lampang ,&nbsp;Chanchana Thanachayanont ,&nbsp;Xun Hu ,&nbsp;Chaoyang Xu ,&nbsp;Shu Zhang","doi":"10.1016/j.indcrop.2025.120792","DOIUrl":"10.1016/j.indcrop.2025.120792","url":null,"abstract":"<div><div>Hydrothermal carbonization of lignocellulosic biomass results in hydrochar with abundant surface functionality and low degree of carbonization. In this work, Cola Shell was subjected to a pretreatment with the Deep Eutectic Solvent (DES) prior to hydrothermal carbonization under different conditions (210 ºC and 260 ºC). The aim was to ascertain the impact of pretreatment with DES on the properties of hydrochars. The results show that pretreatment with DES at 110 ºC favors the disintegration of hemicellulose while promoting the cellulose hydrolysis and dehydration. The lignin removal formed a snow cloud of particles on hydrochar surface, which further undergoes polymerization at a high HTC temperature to form a cluster of fibrous carbonaceous material, contributing to increase the degree of carbonization and thermal stability of hydrochar. DES pretreatment also reduced structural defects while increasing the cellulose crystallinity. In addition, DES pretreatment increased the nitrogen content and energy yield without affecting the calorific value of hydrochar-derived Cola Nut Shell.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"227 ","pages":"Article 120792"},"PeriodicalIF":5.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corrigendum to ‘Phytochemical content of roasted seeds of three white mustard (Sinapis alba L.) varieties differing in their glucosinolate and erucic acid content’ [Ind. Crops Prod. 220 (2024) 119207]
IF 5.6 1区 农林科学
Industrial Crops and Products Pub Date : 2025-03-07 DOI: 10.1016/j.indcrop.2025.120828
Aleksander Siger , Anna Grygier , Ewa Bąkowska , Joanna Szczechowiak-Pigłas , Iwona Bartkowiak-Broda
{"title":"Corrigendum to ‘Phytochemical content of roasted seeds of three white mustard (Sinapis alba L.) varieties differing in their glucosinolate and erucic acid content’ [Ind. Crops Prod. 220 (2024) 119207]","authors":"Aleksander Siger ,&nbsp;Anna Grygier ,&nbsp;Ewa Bąkowska ,&nbsp;Joanna Szczechowiak-Pigłas ,&nbsp;Iwona Bartkowiak-Broda","doi":"10.1016/j.indcrop.2025.120828","DOIUrl":"10.1016/j.indcrop.2025.120828","url":null,"abstract":"","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"227 ","pages":"Article 120828"},"PeriodicalIF":5.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unveiling the influence of NPK, organic fertilizers, and plant growth enhancers on China aster (Callistephus chinensis L.) CV. ‘Arka Kamini’ seed yield
IF 5.6 1区 农林科学
Industrial Crops and Products Pub Date : 2025-03-07 DOI: 10.1016/j.indcrop.2025.120778
S. BharathKumar , P. Prasanth , M. Sreenivas , P. Gouthami , G. Sathish , A.C. Jnanesha , S. Ranjith Kumar , S. Venu Gopal , K. Sravya , Ashish Kumar , R.K. Lal
{"title":"Unveiling the influence of NPK, organic fertilizers, and plant growth enhancers on China aster (Callistephus chinensis L.) CV. ‘Arka Kamini’ seed yield","authors":"S. BharathKumar ,&nbsp;P. Prasanth ,&nbsp;M. Sreenivas ,&nbsp;P. Gouthami ,&nbsp;G. Sathish ,&nbsp;A.C. Jnanesha ,&nbsp;S. Ranjith Kumar ,&nbsp;S. Venu Gopal ,&nbsp;K. Sravya ,&nbsp;Ashish Kumar ,&nbsp;R.K. Lal","doi":"10.1016/j.indcrop.2025.120778","DOIUrl":"10.1016/j.indcrop.2025.120778","url":null,"abstract":"<div><div>The entitled research study carried out at the Agricultural Research Institute's Floricultural Research Station, affiliated with the Agricultural Research Institute in Rajendranagar, in Hyderabad, India, investigated from October 2020 to April 2021 the effects of various fertilizer and biostimulant applications on the growth, flowering, seed yield, and quality of China aster cv. Arka Kamini. The study followed an RCBD with three replications and seven distinct treatment groups. Treatment 6, which consisted of applying the recommended dosage of NPK at 50 % along with Azotobacter, PSB, and KSB biofertilizers and a 0.5 % seaweed extract solution, produced the highest number of flowers/ plant (21.98), flower yield/plant (48.43 g), seed yield/flower (0.30 g), and seed yield/plant (6.59). Treatment 3, RDF at 75 % dosage plus the same biofertilizers and seaweed extract, exhibited the highest number of seeds/gm (484.23 g) and test weight (1.72 kg). Moreover, Treatment 6 demonstrated the maximum germination percentage (90.11 %), shortest days to germination (5.25 days), and highest speed of germination (9.00). Treatment 3 had the lowest mortality rate (15.24 %), best survival rate (84.76 %), seedling length (5.93 cm), and seedling vigor indices 1 and 2 (5.06 and 9.39,), respectively.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"227 ","pages":"Article 120778"},"PeriodicalIF":5.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of different pretreatments on the drying and quality characteristics of Strobilanthes sarcorrhiza (C. Ling) by hot-air drying
IF 5.6 1区 农林科学
Industrial Crops and Products Pub Date : 2025-03-06 DOI: 10.1016/j.indcrop.2025.120759
Shuqing Zhu , Suikang Yuan , Cong Wang , Jiang Wu , Kaijun Wang , Xingquan Liu , Fenghua Wu , Peng Wang
{"title":"Effects of different pretreatments on the drying and quality characteristics of Strobilanthes sarcorrhiza (C. Ling) by hot-air drying","authors":"Shuqing Zhu ,&nbsp;Suikang Yuan ,&nbsp;Cong Wang ,&nbsp;Jiang Wu ,&nbsp;Kaijun Wang ,&nbsp;Xingquan Liu ,&nbsp;Fenghua Wu ,&nbsp;Peng Wang","doi":"10.1016/j.indcrop.2025.120759","DOIUrl":"10.1016/j.indcrop.2025.120759","url":null,"abstract":"<div><div>In this study, the effects of ultrasound (USP), freeze-thaw (FTP), microwave (MWP), and blanching (BLP) pretreatments on the drying kinetic, water migration, microstructure, phytochemical compositions, and antioxidant activities of SSL were investigated. The SSL after BLP with large porous structures had higher effective moisture diffusivity and drying rate than the control with honeycomb structures, and the BLP-HAD group decreased its initial drying time and energy consumption of 51.23 % and 53.09 %, respectively. The SSL treated with BLP-HAD had improved rehydration rate of 2.23 g/g, hardness of 51.52 N, and shrinkage rate of 80 %. Additionally, the SSL after BLP treatment had higher contents of saponin (10.13 g/100 g), total phenolic (64.74 mg/100 g), and total flavonoid (3.95 g/100 g) than the HAD, thereby resulting in enhanced antioxidant activities of BLP-HAD group. BLP combined with hot-air drying is a promising pretreatment technique, as it improves both the drying process and the quality of the SSL.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"227 ","pages":"Article 120759"},"PeriodicalIF":5.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improvement of plant tolerance to salt stress by GhRL192 mRNA through long-distance movement
IF 5.6 1区 农林科学
Industrial Crops and Products Pub Date : 2025-03-06 DOI: 10.1016/j.indcrop.2025.120806
Xinjie Zhang , Wenqian Liu , Tianen Zhang , Jinzhu Li , Jie He , Yuanyuan Liu , Lu Yang , Yuan Wang , Jun Peng , Zhanshuai Li
{"title":"Improvement of plant tolerance to salt stress by GhRL192 mRNA through long-distance movement","authors":"Xinjie Zhang ,&nbsp;Wenqian Liu ,&nbsp;Tianen Zhang ,&nbsp;Jinzhu Li ,&nbsp;Jie He ,&nbsp;Yuanyuan Liu ,&nbsp;Lu Yang ,&nbsp;Yuan Wang ,&nbsp;Jun Peng ,&nbsp;Zhanshuai Li","doi":"10.1016/j.indcrop.2025.120806","DOIUrl":"10.1016/j.indcrop.2025.120806","url":null,"abstract":"<div><div>Salt stress poses a major restricting factor for sustainable agricultural development, limiting crop productivity and adversely affecting crop productivity. Grafting is a commonly used technique to improve the quality and stress resilience of horticultural crops. However, limited research has utilized grafting to explore the role of key mobile mRNAs in response to salt stress for molecular breeding applications. In this study, grafting systems were established between cotton and okra or tobacco. Through these systems, the 60S Ribosomal Protein L19–2 (<em>RL192</em>) mRNA was identified as a mobile molecule moved from cotton (rootstock) to okra (scion). Fluorescence quantification and β-glucuronidase (GUS) staining analyses showed that <em>GhRL192</em> expression was significantly induced by salt stress. Silencing <em>GhRL192</em> in cotton significantly reduced salt stress tolerance, whereas overexpression of <em>GhRL192</em> in tobacco or Arabidopsis improved seed germination and root development and the survival rate of seedlings under salt stress. This novel grafting strategy between horticultural and agricultural crops provides new insights and methodologies for studying mobile mRNAs that enhance crop stress resistance, yield, and quality. The identification and analysis of <em>GhRL192</em> mRNA in relation to salt tolerance confirmed the feasibility of this approach, laying the groundwork for further exploration of transport mechanisms and molecular regulatory networks.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"227 ","pages":"Article 120806"},"PeriodicalIF":5.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143549096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Geographical variations in Polygonatum polysaccharides and saponins biosynthesis in Polygonatum species
IF 5.6 1区 农林科学
Industrial Crops and Products Pub Date : 2025-03-06 DOI: 10.1016/j.indcrop.2025.120814
Zihao Li , Zixuan Hu , Kangyi Zhao , Lin Yang , Huaxuan Tang , Jiaru Li
{"title":"Geographical variations in Polygonatum polysaccharides and saponins biosynthesis in Polygonatum species","authors":"Zihao Li ,&nbsp;Zixuan Hu ,&nbsp;Kangyi Zhao ,&nbsp;Lin Yang ,&nbsp;Huaxuan Tang ,&nbsp;Jiaru Li","doi":"10.1016/j.indcrop.2025.120814","DOIUrl":"10.1016/j.indcrop.2025.120814","url":null,"abstract":"<div><div><em>Polygonatum</em> is an important source of polygonatum polysaccharides (PSPs) and saponins. However, the low environmental adaptability of plants producing bioactive components impedes their industrial development. In this study, three Chinese pharmacopoeias of <em>Polygonatum</em> were collected from three important populations covering a wide geographical area from northwest to south China. Variations in metabolites of PSPs and saponins biosynthesis pathway were determined. Moreover, the specialized metabolites showed regional variations across a large geographical scale. Among the environmental variables assessed, daily precipitation amount and elevation appear to have a significant impact on total saponins content. Similarly, sunshine duration and relative humidity appear to have a significant impact on the variation in PSPs content. Furthermore, correlation analysis of transcriptome and important metabolites reveals differences in the expression of genes related to the biosynthetic pathway of three <em>Polygonatum</em> species. Two potential candidate genes, <em>GPI</em> and <em>CAS</em>, have been identified as being respectively involved in the biosynthesis and regulation of PSPs and saponins. This study provides valuable information to understand the effect of environmental factors and key genes on the production of bioactive chemicals in medicinal crops.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"227 ","pages":"Article 120814"},"PeriodicalIF":5.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabolic pathway for evaluation of polyunsaturated fatty acids content variation and regulation of its biosynthesis in Paeonia ostii through abscisic acid and ABSCISIC ACID-INSENSITIVE 5 (PoABI5)
IF 5.6 1区 农林科学
Industrial Crops and Products Pub Date : 2025-03-06 DOI: 10.1016/j.indcrop.2025.120758
Yang Li , Xuanqi Wang , Shangwei Wu , Xinyan Mei , Wenjin Song , Qingyan Shu
{"title":"Metabolic pathway for evaluation of polyunsaturated fatty acids content variation and regulation of its biosynthesis in Paeonia ostii through abscisic acid and ABSCISIC ACID-INSENSITIVE 5 (PoABI5)","authors":"Yang Li ,&nbsp;Xuanqi Wang ,&nbsp;Shangwei Wu ,&nbsp;Xinyan Mei ,&nbsp;Wenjin Song ,&nbsp;Qingyan Shu","doi":"10.1016/j.indcrop.2025.120758","DOIUrl":"10.1016/j.indcrop.2025.120758","url":null,"abstract":"<div><div><em>Paeonia ostii</em> seed oil, rich in polyunsaturated fatty acids (PUFAs), offers a valuable source of health-promoting vegetable oils. However, the metabolic mechanisms underlying PUFA content variation and biosynthesis regulation remain poorly understood. This study analyzed PUFA variation patterns in seeds from 131 <em>P. ostii</em> plants using gas chromatography-mass spectrometry (GC-MS). To investigate primary metabolite dynamics, ultra-high-performance liquid chromatography (UPLC)-MS was conducted on seeds at key developmental stages 2 and 4, identifying 422 primary metabolites, including 165 differential ones. Pearson correlation analysis revealed that abscisic acid (ABA) was highly associated with fatty acids and their derivatives. Exogenous ABA treatment significantly increased PUFA content in <em>P. ostii</em> seeds. Furthermore, overexpression of <em>ABSCISIC ACID-INSENSITIVE 5</em> (<em>PoABI5</em>) in <em>Arabidopsis thaliana</em> upregulated key genes, including <em>AtFAD2/3</em> and <em>AtWRI1</em>, enhancing PUFA accumulation. This research elucidates key metabolites, particularly PUFAs, in mature seeds of various cultivated plants and developing seeds of <em>P</em>. <em>ostii</em>. Moreover, this study reveals the role of ABA and ABI5 in PUFA biosynthesis and provides a foundation for metabolic engineering to improve oil crop quality, addressing nutritional demands, particularly for populations with metabolic syndromes.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"227 ","pages":"Article 120758"},"PeriodicalIF":5.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143549206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unraveling the floral aroma of lotus (Nelumbo nucifera): Insights from volatile metabolomics and transcriptomics
IF 5.6 1区 农林科学
Industrial Crops and Products Pub Date : 2025-03-06 DOI: 10.1016/j.indcrop.2025.120782
Lin Chen , Heyun Song , Jia Xin , Wei Cheng , Mei Yang , Heng Sun
{"title":"Unraveling the floral aroma of lotus (Nelumbo nucifera): Insights from volatile metabolomics and transcriptomics","authors":"Lin Chen ,&nbsp;Heyun Song ,&nbsp;Jia Xin ,&nbsp;Wei Cheng ,&nbsp;Mei Yang ,&nbsp;Heng Sun","doi":"10.1016/j.indcrop.2025.120782","DOIUrl":"10.1016/j.indcrop.2025.120782","url":null,"abstract":"<div><div>Lotus (<em>Nelumbo nucifera</em>) is a prominent ornamental plant renowned for its delightful floral aroma. We conducted an integrated analysis of volatile metabolomics and transcriptomics to investigate the composition and emission patterns of lotus floral aromas. A total of 1430 volatile organic compounds (VOCs) were identified across different flower organs, with stamens identified as the primary source. Notably, 1072 VOCs were detected in the stamens during various flowering stages, particularly on the day of flowering (S2) and the day after (S3), which were crucial for scent emission. The major contributors to the aroma included terpenoids, esters, and ketones. Transcriptome analysis identified 13,034 differentially expressed genes (DEGs) across various stages of stamen development. These DEGs were predominantly enriched in metabolic pathways and the biosynthesis of secondary metabolites. The expression of DEGs closely correlated with the accumulation profiles of VOCs, which increased during stages S2 and S3, before decreasing at stage S4, two days after flowering. Integrated analysis revealed that specific structural genes from the mevalonate (MVA) and 2-<em>C</em>-methyl-D-erythritol-4-phosphate (MEP) pathways significantly influence terpenoid biosynthesis and floral aroma formation. These findings deepen our understanding of lotus floral aroma and provide a foundation for the genetic improvement of floral aroma traits in lotus.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"227 ","pages":"Article 120782"},"PeriodicalIF":5.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143549036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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