{"title":"A review of analytical techniques coupled with chemometric methods for tobacco origin traceability","authors":"Xiaoyue Yuan, Lili Cui, Shulei Han, Yaning Fu, Hongjuan Wang, Yushan Tian, Xiao Li, Huan Chen, Hongwei Hou, Yuwei Yuan","doi":"10.1016/j.indcrop.2025.122004","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.122004","url":null,"abstract":"Tobacco holds significant economic value and medicinal potential, but illegal trade and counterfeit products pose major barriers to its sustainable development. Tobacco origin traceability technologies offer practical solutions by ensuring product authenticity and quality assurance. Despite the emergence of new technologies, a systematic review of tobacco origin traceability remains lacking. This paper presents the first systematic summary of organic compound fingerprints, mineral element fingerprints, and isotope fingerprints, detailing their respective advantages and limitations. Chemometric methods are introduced, and their integration with these three traceability techniques for tobacco origin identification is discussed. Furthermore, research gaps in blockchain-IoT integration, omics technologies, and spectroscopic techniques are highlighted, with potential integrations with tobacco traceability systems proposed. This review demonstrates that technology integration significantly enhances the accuracy of traceability, offering comprehensive insights into the geographic origin of tobacco.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"114 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aiyuan Xue, Ningke Fan, Le Yue, Xiaona Li, Xie Wang, Huimin Liao, Zhenggao Xiao, Zhenyu Wang
{"title":"Salicylic acid-functionalized nanogels enhance tobacco mosaic virus resistance and alleviate oxidative stress in crops","authors":"Aiyuan Xue, Ningke Fan, Le Yue, Xiaona Li, Xie Wang, Huimin Liao, Zhenggao Xiao, Zhenyu Wang","doi":"10.1016/j.indcrop.2025.122009","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.122009","url":null,"abstract":"Tobacco mosaic virus (TMV), one of the most devastating plant pathogens worldwide, causes catastrophic agricultural losses. While chemical pesticides remain primary method for controlling viral diseases, their improper use poses significant ecological risks, including environmental persistence and biomagnification. Herein, we employed a nanogel carrier to encapsulate salicylic acid (SA), creating a green nanobiostimulant termed salicylic acid-functionalized nanogels (SAN). This SAN exhibited excellent stability, strong foliar adhesion, a favorable slow-release profile, and high biosafety for tadpoles. Foliar application of 50 mg/L SAN resulted in a higher control efficiency against TMV in tobacco (<em>Nicotiana tabacum</em> L.) plants than that of SA or lentinan (LNT, a commercial biostimulant) alone, and the disease severity of SAN was significantly reduced by 29.6 % and 20.5 % compared with SA and LNT, respectively. Mechanistically, 50 mg/L SAN directly inactivated TMV by causing the aggregation and fracture of TMV particles. Furthermore, SAN indirectly induced tobacco plant resistance to TMV via activating endogenous signaling (Ca<sup>2 +</sup> influx and phytohormone accumulation), upregulating the expression of key genes for disease resistance (<em>e.g</em>., <em>PR1</em>, <em>PR3</em>), and enhancing defense metabolites involved phenylpropanoid pathway (<em>e.g</em>., chlorogenic acid, ferulic acid, rutin). Moreover, SAN significantly mitigated tobacco leaf oxidative stress by increasing the activity of antioxidant enzymes (SOD, POD, CAT) and the production of flavonoids, and such effect was better than that of SA and LNT. Overall, SAN is a promising eco-friendly nanobiostimulant with the potential to replace conventional biostimulants and pesticides for sustainable disease control in agroecosystems.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"2 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145204032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Canopy nitrogen addition reshapes leaf physiological-metabolic processes in Populus euphratica seedlings with a water dependency","authors":"Yuehan Liu, Yuchen Wang, Huifang Wang, Eryang Li, Yudong Chen, Qiong Wu, Wen Cao, Guanghui Lv","doi":"10.1016/j.indcrop.2025.122019","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.122019","url":null,"abstract":"Compared to soil nitrogen (N) addition, canopy N addition offers a more ecologically relevant simulation of natural N deposition, and plays a pivotal role in regulating plant physiological metabolism and adaptive strategies. However, it is uncertain whether this critical process is water-dependent. This study systematically revealed the synergistic response mechanism of <em>Populus euphratica</em> seedlings to water treatment (drought stress vs. well-watered) and N addition method (canopy N addition vs. soil N addition). The results showed that: (1) The interactive effects significantly regulated soil nutrient characteristics, with N addition method exerting a pronounced positive effects on ammonium nitrogen (AN) and soil organic carbon (SOC) (<em>P</em> < 0.05); (2) At the leaf physiological level, canopy N addition under drought stress significantly decreased the contents of Proline (LPRO) and Starch (LST), along with declining trends in Malondialdehyde (MDA) levels, Peroxidase (POD) and Ascorbate peroxidase (APX) activities, indicating its capacity to alleviate drought stress; (3) Metabolomic analysis revealed that drought stress induced changes in various secondary metabolites, including Carbohydrates and carbohydrate conjugates (7.55 %), Amino acids, peptides, and analogues (5.03 %), Flavonoid glycosides (4.40 %), enriched in pathways such as Arachidonic acid metabolism and Flavonoid biosynthesis. Canopy N addition markedly expanded drought-responsive metabolites, predominantly influencing primary metabolism (amino acid metabolism); (4) Structural equation modeling identified that water treatment exerted stronger direct effects on differential metabolites (DMs) variations than N addition method, while the latter indirectly drove metabolic reprogramming through modulation of leaf nutrients and soil N availability. These findings unveil desert plants’ balanced strategies of physiological defense and resource acquisition through water-nitrogen coupling, establishing nutrient stewardship frameworks for maintaining arid ecosystem equilibrium under global climatic perturbations scenarios.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"99 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The bZIP transcription factor PrABI5 regulates α-linolenic acid synthesis in tree peony seeds","authors":"Yanfeng Xu, Yihan Ma, Yuhui Zhai, Xinrong Xie, Lixin Niu, Yanlong Zhang","doi":"10.1016/j.indcrop.2025.122013","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.122013","url":null,"abstract":"The tree peony (<em>Paeonia rockii</em>), a traditional ornamental plant in Chinese horticulture, has recently gained significant attention as a promising woody oil crop rich in α-linolenic acid (ALA), an omega-3 fatty acid with multiple industrial value and health benefits. The development of plant resources rich in ALA and the molecular mechanisms of plant ALA synthesis have emerged as research hotspots. We previously identified the key gene <em>PrFAD3</em> encoding fatty acid desaturase, which catalyze linoleic acid (LA) to synthesis ALA. In <em>P. rockii</em>, however, the upstream transcriptional mechanism remained unclear. In this study, we disclosed that a bZIP family transcription factor, PrABI5, is involved in the synthesis of ALA in <em>P. rockii</em> seeds. Present research indicated that PrABI5 activates the expression of <em>PrFAD3</em> by binding to the ABRE element in the promoter. We verified the positive role of PrABI5 in promoting ALA synthesis through gene overexpression and virus-induced gene silencing (VIGS). Additionally, PrABI5 enhances ALA biosynthesis by recruiting PrCAM7, a member of calmodulin protein family, to strengthen transcriptional regulation of the <em>PrFAD3</em>. Collectively, our study provides novel insights into the transcriptional mechanisms of ALA synthesis in plant seeds and the improvement of plant fatty acid composition through molecular biology techniques.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"39 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juan Wu, Ying Ni, Hui Sun, Lingzhong Wan, Sihong Ye, Jiabao Zhu, Xiaonan Deng
{"title":"Hierarchical porous carbon derived from waste cotton linter for high-performance capacitive deionization","authors":"Juan Wu, Ying Ni, Hui Sun, Lingzhong Wan, Sihong Ye, Jiabao Zhu, Xiaonan Deng","doi":"10.1016/j.indcrop.2025.122041","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.122041","url":null,"abstract":"In this study, a series of hierarchical porous carbons (HPCs) were prepared from waste cotton linter via activation with zinc carbonate basic, and their structure-performance relationships were systematically explored in the context of CDI. The results demonstrate that the introduction of Zn salt effectively tailored the pore architecture and surface chemistry of the carbon materials, leading to the formation of hierarchical structures with interconnected micro-, meso-, and macropores. Among the samples, HPC3 exhibited the highest BET surface area (1298 m²/g), outstanding electrochemical performance with a specific capacitance of 111.7 F/g, and a superior SAC of 19.2 mg/g in 1000 mg/L NaCl solution, surpassing that of commercial activated carbon. Post-reaction XPS and TEM analyses confirmed the material’s structural integrity and regeneration capability during cycling. These findings highlight the critical role of pore structure and surface functionalities in enhancing CDI performance and offer new insights into the high-value utilization of biomass wastes for advanced water treatment applications.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"102 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sustainable additive manufacturing: Microstructural evolution and mechanical viability of recycled Flax/PP via fused granular fabrication","authors":"Baoxing Wang, Adragna Pierre-Antoine, Montay Guillaume, Guang Yang, Siyu Zhou","doi":"10.1016/j.indcrop.2025.121997","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.121997","url":null,"abstract":"This study investigates the recyclability of flax fiber-reinforced polypropylene (Flax/PP) composites processed via fused granular fabrication (FGF), with a focus on microstructural evolution, thermal degradation, and mechanical performance under repeated recycling. Flax/PP composites were subjected to six consecutive recycling cycles (R0–R5). Mechanical testing, scanning electron microscopy (SEM), and infrared thermography were performed at each cycle to assess property degradation, while thermal analysis (DSC and TGA) was conducted at selected stages (R0, R2, and R5). Results show a progressive and irreversible decline in mechanical performance beyond two cycles: tensile strength dropped from ∼13 MPa (R0) to ∼7 MPa (R5), and elongation at break fell from ∼9 % to ∼3 %, accompanied by a shift from ductile to brittle failure. SEM observations revealed increasing fiber pull-out, interfacial debonding, and porosity growth from ∼12.5 % to ∼32.6 %, with maximum pore sizes exceeding 400 µm. DSC and TGA results confirmed thermal degradation of both flax fibers and the PP matrix, including decreased crystallinity and reduced thermal stability. By the sixth cycle, severe interlayer delamination and nozzle clogging rendered the material unprintable. These findings elucidate the degradation pathways of natural fiber composites under intensive melt processing and provide a foundation for improving recyclability. Future work will verify whether strategies such as virgin PP blending, optimized processing conditions, and the use of bio-based coupling agents can mitigate the observed thermal and interfacial deterioration.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"19 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shuyu Luan, Suhaib Nisar, Jonathan Phipps, Antonio Ovejero-Pérez, Jason P. Hallett, Pedro Verdía Barbará
{"title":"Optimization of the ionoSolv process for the preservation of pulp fibre dimensions","authors":"Shuyu Luan, Suhaib Nisar, Jonathan Phipps, Antonio Ovejero-Pérez, Jason P. Hallett, Pedro Verdía Barbará","doi":"10.1016/j.indcrop.2025.121996","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.121996","url":null,"abstract":"The pulping industry, dominated by century-old technologies, is highly polluting, making imperative finding more environmentally friendly alternatives. The ionoSolv process, based on the use of low-cost protic ionic liquids, is one of the most promising methods to fractionate lignocellulose with low ecological impact. However, this process has been only optimized to produce sugar-derived chemicals, where fibre quality is not a key factor. Earlier studies have shown the detrimental effect of the standard ionoSolv conditions on fibre length, which renders them unsuitable for fibre applications, including the production of paper. For the first time, this study shows the optimization of the ionoSolv fractionation of <em>Miscanthus</em> to preserve key fibre properties of the pulps, namely fibre dimensions, degree of polymerization and relative molecular mass, while maintaining optimal purity. Different temperatures (150 ºC and 170 ºC), retention times (45–90 min), and acid:base ratios (a:b = 1.00 to a:b = 0.55) were investigated. Fractionation at 170 ºC for 45 min with a reduced acid:base ratio of a:b = 0.86 allowed for maximum delignification (lignin content of 4.4 %) and preservation of fibre length. Pulps recovered under optimal conditions were bleached using a single stage method based on H<sub>2</sub>O<sub>2</sub> increasing pulp purity, achieving optimal decolorization and retaining fibre dimensions comparable to those obtained from a commercial process (Lc(l) = 0.509 mm and Lc(w) = 0.775 vs Lc(l) = 0.540 mm and Lc(w) = 0.975). This highlights the potential of the ionoSolv process as a greener fractionation alternative to produce cellulose fibre materials.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"93 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jiarui Cui, Shubin Cao, Jie Hao, Yao Zhang, Shuang Gao, Jianshe Li, Fatimah Shuang Ma, Longguo Wu
{"title":"Construction of an industrial detection model for tomato organic acids based on low-rank adaptation and spatial feature fusion","authors":"Jiarui Cui, Shubin Cao, Jie Hao, Yao Zhang, Shuang Gao, Jianshe Li, Fatimah Shuang Ma, Longguo Wu","doi":"10.1016/j.indcrop.2025.121909","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.121909","url":null,"abstract":"Tomato (Solanum lycopersicum L.) is not only a globally important crop for human consumption but also a potential source of industrially valuable organic acids. Organic acids such as citric and malic acid have widespread applications in bio-based chemical synthesis, fermentation, biodegradable plastics, and green solvents. In this study, we develop a novel non-destructive framework combining Low-Rank Adaptation (LoRA) with Adaptive Spatial Feature Fusion Network (ASFFN) to accurately predict organic acid content using hyperspectral imaging (HSI). The model integrates advanced feature fusion strategies and robust outlier detection to improve generalizability across tomato varieties. Comparative experiments demonstrate the superior performance of the proposed LoRA-ASFFN over conventional CNN and PLSR baselines. The model enables rapid and precise identification of tomatoes with high organic acid concentrations, providing an efficient pathway for industrial processing and extraction. This research contributes to advancing bio-based chemical supply chains and improving the economic value of tomato crops through data-driven, precision screening techniques.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"53 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Andleeb Tajammal, Sammer Fatima, Huma Qureshi, Tauseef Anwar, Hossam S. El-Beltagi, Ibrokhim Ismoilov, Feruza Tukhtaboeva, Nazih Y. Rebouh, Maryam M. Alomran, Mohd Asif Shah
{"title":"Green-synthesized copper nanoparticles enhance drought tolerance in wheat for sustainable biomass and industrial applications","authors":"Andleeb Tajammal, Sammer Fatima, Huma Qureshi, Tauseef Anwar, Hossam S. El-Beltagi, Ibrokhim Ismoilov, Feruza Tukhtaboeva, Nazih Y. Rebouh, Maryam M. Alomran, Mohd Asif Shah","doi":"10.1016/j.indcrop.2025.121934","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.121934","url":null,"abstract":"Drought stress severely limits the growth, physiological performance, and yield of <em>Triticum aestivum</em> L. (wheat), particularly under water-limited conditions relevant to industrial and bioenergy applications. This study evaluated the potential of green-synthesized copper nanoparticles (CuNPs), produced using <em>Syzygium aromaticum</em> (L.) Merr. & L.M.Perry) (clove) extract, to enhance drought tolerance in four wheat varieties—Dharab-11, Watan-94, Faisalabad-2008, and Faisalabad-2010—selected based on contrasting stress sensitivity and germination performance. Copper nanoparticles with an average crystalline size of 23.6 nm were applied as foliar sprays at 10, 20, and 30 ppm, while drought stress was imposed at the grain-filling stage by withholding irrigation for 15 days, reducing soil moisture to approximately 40 % of field capacity. CuNP supplementation, particularly at 30 ppm, significantly improved growth, physiological, and biochemical traits under drought. Dharab-11 exhibited the tallest plants (87 cm), Watan-94 maintained the largest leaf area (52 cm²), and Watan-94 also produced the longest spikes (22.5 cm). Relative water content increased up to 94 %, while proline accumulation reached 0.78 µmol g⁻¹ fresh weight, reflecting enhanced osmotic adjustment. Total soluble sugars increased up to 0.96 µmol g⁻¹ fresh weight, supporting cellular turgor and metabolic stability under stress. Yield components, including spikelet number and 1,000-grain weight, were also positively influenced. These findings demonstrate that clove-extract-mediated CuNPs provide a novel, eco-friendly approach to mitigate drought-induced morphological, physiological, and biochemical impairments in wheat. Future field trials are recommended to validate scalability, assess nanoparticle persistence in soil, and evaluate potential phytotoxicity at higher concentrations.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"6 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145189384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Regulatory mechanism of anthocyanin biosynthesis in Dendrobium officinale : Integrating metabolome and transcriptome to reveal the potential application for quality improvement","authors":"Chenning Zhao, Jiaqi Ren, Yu Zhang, Qing Xu, Aimin Lv, Liang Wei, Qingsong Shao, Chenfei Lu","doi":"10.1016/j.indcrop.2025.121987","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.121987","url":null,"abstract":"The MYB and bHLH transcription factors could participate in the synthesis of anthocyanins through interaction or upstream-downstream regulation. Anthocyanins, as one of the most important active substances in plants, have high nutritional value of human beings. At present, anthocyanin biosynthesis has been widely studied, however, the regulatory mechanism of anthocyanins in <em>Dendrobium officinale</em> (<em>D. officinale</em>) remain unknow. Here, the anthocyanin components of two different colored cultivars from <em>D. officinale</em> were analyzed by UPLC-MS/MS. We have found that delphinidin and its derivatives were the main anthocyanin components in <em>D. officinale</em>, among which Malvidin-3-O-glucoside and Malvidin-3-O-galactoside were the main active substances. Subsequently, the key structural genes <em>DoCHS1/2</em>, <em>DoF3H</em>, <em>DoF3’H</em>, <em>DoDFR1/2</em>, <em>DoANS</em>, and transcription factors <em>DoMYB5–1</em>, <em>DoMYB7</em>, <em>DobHLH5–1</em> were identified based on transcriptomic analysis, which were mainly involved in the anthocyanin biosynthesis. Among that, both <em>DoMYB5–1</em> and <em>DoMYB7</em> could interact with <em>DobHLH5–1</em> to form complexes (<em>DoMYB5–1-DobHLH5–1</em>, <em>DoMYB7-DobHLH5–1</em>), and then regulated the expression of <em>DoDFR1</em> and <em>DoF3’H</em> to promote anthocyanin synthesis through the protein-protein interaction, transient overexpression experiments and Y1H assay. Furthermore, we also found that the interaction of <em>DoMYB5–1</em> and <em>DobHLH5–1</em> could cascade-rergulate the complex <em>DoMYB7-DobHLH5–1</em>, thereby regulating the expression of <em>DoDFR1</em> and then participating in the regulation of anthocyanin metabolism in <em>D. officinale</em>. Overall, this study clarified the regulatory mechanism of anthocyanin accumulation in <em>D. officinale</em>, which was helpful to reveal the biological mechanism of secondary metabolism in plants and provided a theoretical reference for the quality improvement of <em>D. officinale</em>.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"32 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145189389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}