Industrial Crops and Products最新文献

{"title":"β-glucosidase mediates ginsenoside hydrolysis in the leaves of Panax species","authors":"Li-Juan Ma, Jia-Yue Liu, Wen-Yu Fu, Hui-Chang Bi, Ding Yuan, Jian-Bo Wan","doi":"10.1016/j.indcrop.2025.120636","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.120636","url":null,"abstract":"Several species of <em>Panax</em> leaves have been traditionally used as folk medicines and food supplements. Previous studies have demonstrated that the hydrolysis of ginsenosides in <em>P. notoginseng</em> leaves occurs when extracted with water as the extraction solvent, rather than with methanol. However, the occurrence of ginsenoside hydrolysis in other congeneric <em>Panax</em> species remains largely unexplored. In this study, twenty-four ginsenosides were simultaneously quantified by liquid chromatography-mass spectrometry in methanol and aqueous extracts from the leaves of eight different <em>Panax</em> species. These findings revealed that 20(<em>S</em>)-protopanaxadiol saponins in <em>Panax</em> leaves undergo hydrolysis, resulting in the formation of deglycosylated products through regioselective cleavage of the common <em>β</em> (1→2) glycosidic bond at C-3 position. This ginsenoside hydrolysis was confirmed to be associated with total proteins extracted from <em>Panax</em> leaves and is directly mediated by <em>β</em>-glucosidases. Collectively, the extraction solvent profoundly influences the ginsenoside profiles of <em>Panax</em> leaves, and ginsenoside hydrolysis is widespread across various <em>Panax</em> species. These findings provide a scientific foundation highlighting the considerable practical implications of <em>Panax</em> leaves and their related products.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"134 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143124399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient removal of lignin in tobacco stems with choline chloride-based deep eutectic solvents","authors":"Saibo Yu, Bingjie Qiu, Yong Jin, Yu Zhao, Wei Luo, Xinhua Qi","doi":"10.1016/j.indcrop.2025.120634","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.120634","url":null,"abstract":"Tobacco stems are a by-product of the tobacco industry and a valuable natural resource. To enhance the quality of tobacco stems, the efficiency of five binary and ternary deep eutectic solvents (DES) composed of choline chloride (ChCl), ethylene glycol (EG), urea, lactic acid (LA), and Gamma-Valerolactone (GVL) for the pretreatment of tobacco stems was investigated with the aim of reducing the lignin content. The experimental analysis showed that all the prepared DES were effective in the removal of lignin from tobacco stems. In particular, the ternary DES system composed of ChCl, LA, and EG ([Ch][LA]₅[EG]_0.6) was the most effective for the pretreatment of tobacco stems, achieving a lignin removal efficiency of 85.9 % at 100°C for 4 h. Under these conditions, a tobacco stem residue rich in cellulose fractions was obtained. The pretreatment of tobacco stems with DES can effectively reduce the lignin content in the stems and the phenolics in the combustion smoke of the stems after delignification, leading to an improvement in the sensory quality of tobacco flake cigarette products.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"40 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143192538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic boosting furoic acid and glucose production from corn stover by tandem chemocatalysis and biocatalysis","authors":"Lirong Huang, Yuehua Chen, Jia Ouyang, Zhaojuan Zheng","doi":"10.1016/j.indcrop.2025.120651","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.120651","url":null,"abstract":"The synthesis of bio-based compounds derived from biomass is essential for promoting sustainable development and environmental preservation. In this study, a tandem chemocatalysis and biocatalysis approach was proposed to co-produce furoic acid (FA) and glucose from corn stover. The corn stover was pretreated using 2 wt% lactic acid (LA), achieving the highest xylose and xylooligosaccharides (XOS) overall yield of 86.1 %. The hydrolysate was catalyzed in a biphasic system by 0.25 % Hβ zeolite and pre-existing LA to obtain furfural, which was further converted into FA at 70.5 % yield in the same biphasic system using biocatalyst of <em>Pseudomonas rhodesiae</em>. In addition, the possible mechanisms of the associated catalytic reactions were also discussed and the enzymatic saccharification efficiency of the solid remains was enhanced to 87.5 %, increased by 3.3 times than that of the original corn stover. More importantly, the glucose obtained could serve as a substrate for cell cultivation, facilitating the production of both biocatalyst (<em>P. rhodesiae</em>) and chemical catalyst (lactic acid). This facile biorefinery method of co-production of FA and glucose improves the bioavailability of corn stover in a greener and simpler manner, avoiding strong acids and complex chemical catalysts.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"40 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143192679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional magnetic cellulose aerogel nanopaper made from nanofibrillated bamboo pulp fiber as templates","authors":"Kangkang Zhang, Lin Liu, Siyu Cui, Xian Wang, Ning Li, Changzhao Li, Chunwang Yang, Chenyang Fan, Jun Li, Yushan Yang, Jian Qiu","doi":"10.1016/j.indcrop.2025.120642","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.120642","url":null,"abstract":"Elastic or ductile networks derived from plant cellulose nanofibrils are suitable for use as nanostructured biofunctional materials with tunable mechanical properties. However, the non-destructive processing of functional guest nanoparticles into macroscopic cellulose nanofibril aerogel nanocomposites with enriched architecture and functionality through conventional nanotechniques maintains a challenge. Here, we developed cellulose nanofibril aerogels that can be used as a versatile template for the nonagglomerative in situ growth of nanoparticles and processed ferromagnetic cobalt ferrite nanoparticles into lightweight and porous magnetic aerogels through their unique network architecture, which were subsequently piezo-controlled into flexible magnetic nanopapers. It optimizes the tensile strength, resistance to ultrasonic decomposition, and ultra-foldable conductivity of flexible magnetic cellulose nanopaper. This flexible magnetic cellulose aerogel nanopaper (CANP) have excellent mechanical strength, with a high tensile modulus of 761.95 ± 10.2 MPa and exceptional foldability without misconfiguration damage after 10,000 repetitions of folding, as well as flexural fatigue resistance. Simultaneously, the highly flexible magnetic CANP exhibits large coercivity and moderately strong saturation magnetization strength. Due to optimized impedance matching of the dielectric/magnetic components with cellulose aerogel, the highly flexible magnetic CANP doped with cobalt ferrite nanoparticles has good effective absorption bandwidth (13.28 GHz) with a minimum reflection loss values of - 58.24 dB. Moreover, it had large coercivities, high saturation magnetization, and a minimum value of reflection.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"138 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143192684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-precision identification transgenic sugarcane using active terahertz low-frequency excitation","authors":"Shan Tu, Zhongzhou Song, Senhao Pang, Qilin He, Jingkai Su, Cheng Zhang, Heng Xiao, Jungang Wang, Xihui Liu, Wentao Zhang, Junhui Hu","doi":"10.1016/j.indcrop.2025.120621","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.120621","url":null,"abstract":"This study proposes a novel, environmentally friendly, accurate, rapid, and cost-effective technique for the classification and identification of transgenic sugarcane, combining terahertz (THz) time-domain spectroscopy with an Isomap-Deep Convolutional Neural Network (Isomap-DCNN). Different types of transgenic sugarcane derived from the same receptor material exhibit similar THz absorbance spectra, posing significant challenges for accurate categorization. The Tukey window function was first applied to the THz absorbance spectra of five transgenic sugarcane varieties, and the processed data were subsequently used for feature extraction with t-distributed Stochastic Neighbor Embedding (t-SNE), LargeVis (LV), and Isomap. To enhance the robustness and generalization capability of the model, the Kennard-Stone (KS) algorithm was employed to partition the training and testing sets. The processed key \"fingerprint\" features were then input into a Decision Tree (DT) classifier and a Deep Convolutional Neural Network (DCNN) classifier, respectively. Notably, the Isomap-DCNN model achieved 100 % accuracy in identifying the five transgenic sugarcane varieties. The Isomap-DCNN model, constructed based on THz spectroscopy, is expected to provide a fast, high-precision, environmentally friendly, and cost-effective technique for the classification and identification of transgenic sugarcane. This approach will aid in the development and implementation of regulatory standards, enhance the safety and efficacy of transgenic sugarcane, and promote public transparency regarding information about transgenic sugarcane.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"56 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143124401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive models for the tensile strength of polymer composites comprising spherical nano-starch using interphase properties","authors":"Muhammad Montazeri, Mohsen Mohammadi, Yasser Zare, Kyong Yop Rhee","doi":"10.1016/j.indcrop.2025.120655","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.120655","url":null,"abstract":"This study presents two novel predictive equations based on the Pukanszky and Leidner–Woodhams models, tailored to forecast the tensile strength of polymer composites containing spherical nano-starch. The developed models integrate factors such as the starch volume fraction (&lt;span&gt;&lt;span style=\"\"&gt;&lt;/span&gt;&lt;span data-mathml='&lt;math xmlns=\"http://www.w3.org/1998/Math/MathML\"&gt;&lt;msub is=\"true\"&gt;&lt;mrow is=\"true\"&gt;&lt;mi is=\"true\"&gt;&amp;#x3D5;&lt;/mi&gt;&lt;/mrow&gt;&lt;mrow is=\"true\"&gt;&lt;mi mathvariant=\"normal\" is=\"true\"&gt;f&lt;/mi&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;' role=\"presentation\" style=\"font-size: 90%; display: inline-block; position: relative;\" tabindex=\"0\"&gt;&lt;svg aria-hidden=\"true\" focusable=\"false\" height=\"2.432ex\" role=\"img\" style=\"vertical-align: -0.697ex;\" viewbox=\"0 -747.2 959.9 1047.3\" width=\"2.229ex\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"&gt;&lt;g fill=\"currentColor\" stroke=\"currentColor\" stroke-width=\"0\" transform=\"matrix(1 0 0 -1 0 0)\"&gt;&lt;g is=\"true\"&gt;&lt;g is=\"true\"&gt;&lt;g is=\"true\"&gt;&lt;use xlink:href=\"#MJMATHI-3D5\"&gt;&lt;/use&gt;&lt;/g&gt;&lt;/g&gt;&lt;g is=\"true\" transform=\"translate(596,-242)\"&gt;&lt;g is=\"true\"&gt;&lt;use transform=\"scale(0.707)\" xlink:href=\"#MJMAIN-66\"&gt;&lt;/use&gt;&lt;/g&gt;&lt;/g&gt;&lt;/g&gt;&lt;/g&gt;&lt;/svg&gt;&lt;span role=\"presentation\"&gt;&lt;math xmlns=\"http://www.w3.org/1998/Math/MathML\"&gt;&lt;msub is=\"true\"&gt;&lt;mrow is=\"true\"&gt;&lt;mi is=\"true\"&gt;ϕ&lt;/mi&gt;&lt;/mrow&gt;&lt;mrow is=\"true\"&gt;&lt;mi is=\"true\" mathvariant=\"normal\"&gt;f&lt;/mi&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt;&lt;/span&gt;&lt;script type=\"math/mml\"&gt;&lt;math&gt;&lt;msub is=\"true\"&gt;&lt;mrow is=\"true\"&gt;&lt;mi is=\"true\"&gt;ϕ&lt;/mi&gt;&lt;/mrow&gt;&lt;mrow is=\"true\"&gt;&lt;mi mathvariant=\"normal\" is=\"true\"&gt;f&lt;/mi&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/script&gt;&lt;/span&gt;), interphase strength (&lt;span&gt;&lt;span style=\"\"&gt;&lt;/span&gt;&lt;span data-mathml='&lt;math xmlns=\"http://www.w3.org/1998/Math/MathML\"&gt;&lt;msub is=\"true\"&gt;&lt;mrow is=\"true\"&gt;&lt;mi is=\"true\"&gt;&amp;#x3C3;&lt;/mi&gt;&lt;/mrow&gt;&lt;mrow is=\"true\"&gt;&lt;mi is=\"true\"&gt;i&lt;/mi&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;' role=\"presentation\" style=\"font-size: 90%; display: inline-block; position: relative;\" tabindex=\"0\"&gt;&lt;svg aria-hidden=\"true\" focusable=\"false\" height=\"1.74ex\" role=\"img\" style=\"vertical-align: -0.582ex;\" viewbox=\"0 -498.8 915.8 749.2\" width=\"2.127ex\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"&gt;&lt;g fill=\"currentColor\" stroke=\"currentColor\" stroke-width=\"0\" transform=\"matrix(1 0 0 -1 0 0)\"&gt;&lt;g is=\"true\"&gt;&lt;g is=\"true\"&gt;&lt;g is=\"true\"&gt;&lt;use xlink:href=\"#MJMATHI-3C3\"&gt;&lt;/use&gt;&lt;/g&gt;&lt;/g&gt;&lt;g is=\"true\" transform=\"translate(571,-150)\"&gt;&lt;g is=\"true\"&gt;&lt;use transform=\"scale(0.707)\" xlink:href=\"#MJMATHI-69\"&gt;&lt;/use&gt;&lt;/g&gt;&lt;/g&gt;&lt;/g&gt;&lt;/g&gt;&lt;/svg&gt;&lt;span role=\"presentation\"&gt;&lt;math xmlns=\"http://www.w3.org/1998/Math/MathML\"&gt;&lt;msub is=\"true\"&gt;&lt;mrow is=\"true\"&gt;&lt;mi is=\"true\"&gt;σ&lt;/mi&gt;&lt;/mrow&gt;&lt;mrow is=\"true\"&gt;&lt;mi is=\"true\"&gt;i&lt;/mi&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt;&lt;/span&gt;&lt;script type=\"math/mml\"&gt;&lt;math&gt;&lt;msub is=\"true\"&gt;&lt;mrow is=\"true\"&gt;&lt;mi is=\"true\"&gt;σ&lt;/mi&gt;&lt;/mrow&gt;&lt;mrow is=\"true\"&gt;&lt;mi is=\"true\"&gt;i&lt;/mi&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/script&gt;&lt;/span&gt;&lt;em&gt;),&lt;/em&gt; interphase Thickness (&lt;em&gt;t&lt;/em&gt;&lt;sub&gt;i&lt;/sub&gt;), and starch radius (&lt;em&gt;r&lt;","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"40 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143192687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a rapid time-resolved fluorescence immunoassay for cannabidiol quantification in Cannabis sativa L","authors":"Ziyan Fan, Xianjie Cai, Xiaojie Xu, Xiaokang Cui, Lin Zhao, Jian Chen, Sheng Xing, Bo Ding, Lihong Ren, Xiaoli Meng, Yuan Ji, Gangling Tang","doi":"10.1016/j.indcrop.2025.120626","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.120626","url":null,"abstract":"As a main cannabinoid of <em>Cannabis sativa</em> L., cannabidiol is widely found in cosmetics, vaping liquid, and food. This study aimed to develop a quantitative test strip based on time-resolved fluorescence immunoassay for onsite cannabidiol detection. A monoclonal antibody was prepared against a novel cannabidiol hapten-protein conjugate, with a half maximal effective concentration of 17.90 ng ml⁻¹ and a detection limit of 2.66 ng ml⁻¹. Based on the monoclonal antibody, a test strip was constructed and validated for the detection of cannabidiol in the cannabis plant. The detection limit was 9.39 ng ml⁻¹ and the detection range was 21.62–374.24 ng ml⁻¹. The monoclonal antibody proved high specificity as showing no cross-reactivity with nine common cannabinoids including delta-8-tetrahydrocannabinol, delta-9-tetrahydrocannabinol, cannabichromene, cannabigerol, cannabinol, cannabidivarin, cannabicyclol, cannabicitran, and cannabidiphorol. Spike/recovery test and analysis of real samples demonstrated that the test strip is an accurate, sensitive, and specific detection technique for cannabidiol identification in <em>Cannabis</em>.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"8 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143124899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the regulatory network of valerenic acid biosynthesis in Valeriana officinalis hairy roots under different light qualities: A coexpression perspective","authors":"Arash Mokhtari, Morteza Ebrahimi, Pejman Azadi, Mehran Shariatpanahi, Rasoul Amirian, Ahmad Sobhani, Mozhdeh Shafaie","doi":"10.1016/j.indcrop.2025.120625","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.120625","url":null,"abstract":"Valerenic acid, a potent antistress sesquiterpene, is commercially valuable. This study employed integrated transcriptomic and metabolomic analysis to identify critical genes and transcription factors (TFs) that regulate valerenic acid biosynthesis in hairy roots of <em>Valeriana officinalis</em> under different LED light qualities. Blue light induced a significant up-regulation of the TFs R2R3 MYB and WRKY1 on day 2 (up to 4.99 and 5.89 times, respectively), while red light specifically affected MYB, TPS3 (day 1) and MYCS (day 4). Interestingly, BHLH emerged as a central TF that interacts directly with most pathway genes, suggesting a complex light-mediated regulation. Our results reveal a potential role for light in modulating the mevalonate (HMGR) and membrane epitope (MEP) pathways, potentially increasing metabolic flux toward terpene biosynthesis. Furthermore, we identified the terpene synthases VoTPS2 and VoTPS5 as critical for valerenic acid production, with TF MYCA acting as a critical activator. In addition, the TFs R2R3 MYB and WRKY1 may play an important role in the regulation of terpene biosynthesis. Remarkably, blue and red-light treatments induced valerenic acid accumulation in hairy roots (0.93 and 0.75 mg/g DW, respectively) within only two days. This discovery not only opens doors for targeted metabolic engineering based on the revealed co-expression network but also holds immense economic potential for valerenic acid-dependent industries.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"1 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143192685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive genome-wide characterization of calmodulin-binding protein 60 gene family reveals PtCBP60E1 enhances the pathogen resistance in poplar","authors":"Ruen Yu, Jiahao Liu, Xiaoqian Yang, Jing Wang, Yanwei Wang","doi":"10.1016/j.indcrop.2025.120601","DOIUrl":"https://doi.org/10.1016/j.indcrop.2025.120601","url":null,"abstract":"The calmodulin-binding protein 60 (CBP60) family members play pivotal roles in plant immunity in plants. Most studies have concentrated on <em>AtCBP60a</em>, <em>AtCBP60b</em> and <em>AtCBP60g</em> in <em>Arabidopsis thaliana</em>. However, there are few reports on the <em>CBP60</em> gene family in woody plants, particularly the function of <em>CBP60e</em> in plant-pathogen interactions. In this investigation, 14 <em>Populus trichocarpa</em> (<em>Pt</em>) <em>CBP60</em> (<em>PtCBP60</em>) genes were identified and these genes were unevenly dispersed across eight chromosomes. The detection of collinearity and the presence of tandem gene clusters suggested that segmental duplication and tandem duplication might be the critical impetus for expanding gene members in poplar. Notably, <em>PtCBP60E1</em> and <em>PtCBP60E2</em> not only showed segmental duplication events, but shared similar physicochemical properties. <em>PtCBP60E1</em> was further evaluated by the temporal expression pattern analysis at different hours post-inoculation (hpi) with <em>Colletotrichum gloeosporioides</em>, which showed that its expression was significantly induced (9.7-fold) at 24 hpi. Intriguingly, the area of leaf lesions (2.8 mm²) in overexpression (OE) of <em>PtCBP60E1</em> (OE-31) lines was notably smaller than that (15.35 mm²) in non-transgenic poplar 84 K (wild-type, WT) lines at 3 days post-inoculation. Higher ROS accumulation was detected in OE lines after <em>C. gloeosporioides</em> infection compared to WT lines. These results showed that <em>PtCBP60E1</em> positively regulated the resistance response of poplar to <em>C. gloeosporioides</em> infection. Overall, this study provided a comprehensive characterization of the <em>PtCBP60</em> gene family in poplar and identified <em>PtCBP60E1</em> as an essential regulatory gene in response to <em>C. gloeosporioides</em> infection, offering new insights into the genomic evolution and molecular mechanisms of poplar <em>CBP60</em>.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"12 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143192680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of 5 MYB transcription factors regulating the polydatin biosynthesis in Polygonum cuspidatum Sieb. et Zucc","authors":"Hongping Ma,&nbsp;Shenglong Chen,&nbsp;Fan Lin,&nbsp;Jianhui Chen,&nbsp;Haili Fan,&nbsp;Tong Zhang,&nbsp;Xiangqi Kong,&nbsp;Siqi Zhang,&nbsp;Tuanyao Chai,&nbsp;Hong Wang","doi":"10.1016/j.indcrop.2025.120612","DOIUrl":"10.1016/j.indcrop.2025.120612","url":null,"abstract":"<div><div>Polydatin, a glucoside of resveratrol, has improved bioavailability and higher anti-inflammatory, antioxidant, and anti-apoptotic activities. Currently, the regulatory mechanism of polydatin biosynthesis remains largely unknown. We previously identified a glycosyltransferase PcUGT95D10 which could catalyze the formation of polydatin from <em>Polygonum cuspidatum</em> Sieb. et Zucc., a main source of polydatin in industrial scale. This study is to further investigate its catalytic mechanism and transcriptional regulatory mechanism for resveratrol. PcUGT95D10 exhibited sugar donor promiscuity, and the residues around active pocket, especially the Gly161, played key roles in the glycosylation. By comparative analysis of transcriptome and metabolome, along with Y1H assay, 5 MYB transcription factors (PcMYB1, PcMYB10, PcMYBR01, PcMYB114, and PcMYB306) linked to <em>PcUGT95D10</em> expression were characterized. The identical or opposite expression patterns of the 5 PcMYBs in different tissues with <em>PcUGT95D10</em> gene and polydatin content revealed their regulatory effects on the polydatin biosynthesis. PcMYB1, PcMYB10, PcMYBR01, PcMYB114, and PcMYB306 all could specifically bind to the AC-box element in <em>PcUGT95D10</em> promoter, with PcMYB1, PcMYB10, and PcMYBR01 as activators to enhance the promoter activity, while PcMYB114 and PcMYB306 playing the inhibiting role. In addition, overexpressing <em>PcMYB1</em>, <em>PcMYB10</em>, or <em>PcMYBR01</em> increased the polydatin accumulation in <em>P. cuspidatum</em>, while the polydatin content was reduced in transgenic <em>PcMYB114</em>-overexpressed and <em>PcMYB306</em>-overexpressed hairy roots. In sum, this study successfully identified the potential key residues of PcUGT95D10 glycosylating resveratrol and elucidated a regulatory network of MYB transcription factors for polydatin biosynthesis in <em>P. cuspidatum</em>, which offers new insights into the molecular mechanism of polydatin biosynthesis in <em>P. cuspidatum.</em></div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"226 ","pages":"Article 120612"},"PeriodicalIF":5.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}