Yin Xiong, Xiaoyan Che, Chao Li, Yindi Zhu, Changhao Li, Yanan Zi, Xiuming Cui
{"title":"Temperature-dependent saponin transformation and process-linked quality formation during steaming of Panax notoginseng","authors":"Yin Xiong, Xiaoyan Che, Chao Li, Yindi Zhu, Changhao Li, Yanan Zi, Xiuming Cui","doi":"10.1016/j.indcrop.2026.124543","DOIUrl":"10.1016/j.indcrop.2026.124543","url":null,"abstract":"<div><div>Panax notoginseng (Burk.) F. H. Chen is a cultivated medicinal crop whose post-harvest steaming process markedly alters its major saponin markers in roots. In this study, the temperature-dependent transformation of saponins during steaming was systematically investigated to clarify process-linked quality formation in Panax notoginseng root. Samples were steamed at 105, 110, and 120 °C for 2–10 h, and major native and newly generated saponins were quantitatively profiled. Steaming caused a pronounced shift from native saponins to newly generated less-polar products, with 120 °C showing the most pronounced marker remodeling within the tested temperature range. Protopanaxadiol- and protopanaxatriol-type saponins exhibited distinct transformation patterns, indicating differential pathway responses to the tested steaming conditions. Molecular dynamics and density functional theory analyses further provided comparative evidence that elevated temperature was associated with looser intermolecular organization and lower relative thermodynamic requirements for several downstream transformation steps. These results provide a process-oriented framework for understanding steaming-induced quality formation in Panax notoginseng and may support post-harvest process control and chemical standardization of steamed materials.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"252 ","pages":"Article 124543"},"PeriodicalIF":6.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149142081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Di Wu, Peipei Chen, Xiaoyi Fu, Mengting Ren, Aizhong Liu, Wei Xu, Bing Han
{"title":"AFL transcription factors play crucial roles for ricinoleic acid and storage lipid biosynthesis in castor bean seeds","authors":"Di Wu, Peipei Chen, Xiaoyi Fu, Mengting Ren, Aizhong Liu, Wei Xu, Bing Han","doi":"10.1016/j.indcrop.2026.124528","DOIUrl":"10.1016/j.indcrop.2026.124528","url":null,"abstract":"<div><div>AFL transcription factors (ABI3, FUS3, and LEC2) are key regulators of seed development and lipid metabolism in plants; however, their regulatory network and target genes remain poorly characterized in castor bean (<em>Ricinus communis</em>), an important oil crop. This study aimed to elucidate the regulatory role of RcAFL transcription factors in triacylglycerol (TAG) and hydroxy fatty acid biosynthesis in castor bean seeds. Using DNA affinity purification sequencing (DAP-seq), we detected the whole-genome binding sites of RcABI3, RcFUS3, and RcLEC2, identifying 775 potential target genes including 62 lipid-related genes. Electrophoretic mobility shift assays (EMSA) revealed distinct <em>cis</em>-regulatory element preferences: RcFUS3 bound canonical RY motifs (5′-CATGCA-3′), RcLEC2 recognized RY-like motifs (5′-CATGNNCATGCA-3′), and RcABI3 targeted a novel AGCAATGA motif. Yeast one-hybrid and luciferase reporter assays validated direct binding and transcriptional activation of key lipid-related genes by RcFUS3, including a gene encoding FAH12 critical for ricinoleic acid synthesis. Furthermore, RcFUS3 directly activated the expression of RcWRI1, a master regulator of oil biosynthesis, establishing a hierarchical regulatory loop that amplifies the lipid biosynthesis pathway. Importantly, transient overexpression of <em>RcFUS3</em> in castor bean cotyledon protoplasts significantly increased TAG content, and ChIP-qPCR confirmed that RcFUS3 directly binds to the RY motifs in the promoters of <em>Oleosin2</em>, <em>PDAT2</em>, and <em>RcWRI1</em> in vivo. These findings demonstrate that RcAFL transcription factors coordinate TAG biosynthesis in castor bean seeds by binding distinct <em>cis</em>-elements, with RcFUS3 serving as a key regulator of lipid-related genes, providing valuable information toward understanding the potential regulatory network of AFL in castor beans.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"252 ","pages":"Article 124528"},"PeriodicalIF":6.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149142197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xin Xu, Yuru Ma, Zixin Gai, Qingxia Zheng, Ting Liu, Aiguo Yang, Xiaye Chen, Lirui Cheng, Jun Yang
{"title":"GWAS and functional validation identify NtJMJ as a positive regulator of nicotine accumulation in Nicotiana tabacum","authors":"Xin Xu, Yuru Ma, Zixin Gai, Qingxia Zheng, Ting Liu, Aiguo Yang, Xiaye Chen, Lirui Cheng, Jun Yang","doi":"10.1016/j.indcrop.2026.124550","DOIUrl":"10.1016/j.indcrop.2026.124550","url":null,"abstract":"<div><div>Nicotine is an important metabolite in tobacco. However, the genetic and epigenetic bases underlying its natural variation remain poorly characterized. In this study, a multi-parent advanced generation intercross (MAGIC) population was used to dissect the genetic architecture of nicotine content across two environments. A total of 61,421 single nucleotide polymorphisms (SNPs) were used in genome wide association study, which identifyied 52 significant loci associated with nicotine accumulation. Among these, a stable locus on chromosome 2 (<em>qNIC2</em>) was consistently detected across environments and overlapped with previously reported nicotine-related quantitative trait loci (QTL). To identify the candidate gene within the <em>qNIC2</em> interval, expression profile data from multiple tobacco tissues were integrated and analyzed using co-expression network analysis. A root enriched co-expression module containing all known nicotine biosynthesis and regulatory genes was identified. Within this module, <em>NtJMJ</em>, encoding a putative JmjC domain-containing histone demethylase, was selected as the candidate gene due to its strong root-specific and topping-inducible expression pattern. Genotype groups based on marker AX-117702345 showed significant differences in nicotine content, with TT accessions showing higher <em>NtJMJ</em> expression and consequently higher nicotine content. Subcellular localization analysis revealed the nuclear localization of NtJMJ, supporting its potential role as a transcriptional regulator. Functional validation further indicated that <em>NtJMJ</em> positively regulates nicotine accumulation, as edited lines showed reduced nicotine accumulation and overexpression lines increased it. Consistently, <em>PMT</em> expression was upregulated in overexpression lines and downregulated in mutant lines. Notably, phylogenetic and protein structural analyses indicated that NtJMJ belongs to the KDM4 subfamily and possesses a highly conserved JmjC catalytic core and trimethyl-lysine binding pocket, supporting its annotation as a putative histone demethylase. Collectively, our findings demonstrate that <em>NtJMJ</em> contributes to nicotine accumulation in tobacco, providing new insights into nicotine metabolism and offering a promising molecular target for breeding.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"252 ","pages":"Article 124550"},"PeriodicalIF":6.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149200462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Natural rubber latex preservation as a coupled microbial–colloidal process: From ammonia preservation to emerging alternatives","authors":"Yazhong Song, Maneephan Sukkho, Narueporn Payungwong, Long Han, Wending Zhou, Peng Xing, Xijuan Li, Zheng Peng, Mingzhe Lyu, Hongxing Gui, Jihua Li, Jitladda Sakdapipanich","doi":"10.1016/j.indcrop.2026.124577","DOIUrl":"10.1016/j.indcrop.2026.124577","url":null,"abstract":"<div><div>Natural rubber latex (NRL) preservation controls a biologically active colloidal system, not just microbial spoilage. Rubber particles coexist with proteins, lipids, carbohydrates, mineral ions, enzymes, and microorganisms; changes in the serum phase and particle interface can therefore affect storage stability, processing behavior, and product performance. This review examines relationships among microbial activity, acid formation, interfacial stabilization, and processing compatibility, using ammonia-preserved latex as the reference system. Conventional, low-ammonia, and ammonia-free strategies are compared separately for field latex, latex concentrates, and compounded or pre-vulcanized latex. Representative published data are evaluated according to formulation, dosage basis, observation period, and level of validation, rather than being treated as directly comparable rankings. Experimentally supported relationships are distinguished from the authors’ conceptual integration of these relationships. Organic, biobased, nano-enabled, and acidic systems have been investigated at different levels of experimental and application-specific validation; however, storage stability or ammonia removal alone does not demonstrate industrial readiness or environmental superiority. Particular attention is given to preservative residues, wastewater compatibility, standard-method applicability, and the distinction between laboratory processing tests, reported production use, and comprehensive industrial qualification. An incremental cost–benefit framework and a milestone-based validation pathway are proposed to connect preservation research with practical adoption. Key priorities include quantitative microbial–interfacial relationships, preservative partitioning, reproducible process performance, and application-specific safety and environmental assessment. Successful alternatives should therefore be evaluated against these combined requirements rather than preservation time alone.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"252 ","pages":"Article 124577"},"PeriodicalIF":6.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jaume Sempere-Torregrosa,Harrison de la Rosa-Ramírez,Santiago Ferrándiz Bou,María Dolores Samper
{"title":"Bio-based plasticization of PLA-PHB blends using chemically modified borage seed oils towards sustainable active materials","authors":"Jaume Sempere-Torregrosa,Harrison de la Rosa-Ramírez,Santiago Ferrándiz Bou,María Dolores Samper","doi":"10.1016/j.indcrop.2026.124437","DOIUrl":"https://doi.org/10.1016/j.indcrop.2026.124437","url":null,"abstract":"","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"100 1","pages":"124437"},"PeriodicalIF":5.9,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149022477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shanyu Han,Ziyang Zhang,Jiahuan Feng,Xinyan Hu,Ge Wang
{"title":"Fabrication of a highly porous, hydrophobic, and oleophilic bamboo sponge via a three-dimensional porous bamboo fiber network reconstruction","authors":"Shanyu Han,Ziyang Zhang,Jiahuan Feng,Xinyan Hu,Ge Wang","doi":"10.1016/j.indcrop.2026.124536","DOIUrl":"https://doi.org/10.1016/j.indcrop.2026.124536","url":null,"abstract":"","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"38 1","pages":"124536"},"PeriodicalIF":5.9,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149151576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Research on the construction and application of diatomite-based functionalized bamboo fiber/HDPE composite materials","authors":"Shiyuan Wang,Tao Zhang,Xin Wei,Haiqiao Zhang,Zhong Zhang,Yuxia Chen","doi":"10.1016/j.indcrop.2026.124532","DOIUrl":"https://doi.org/10.1016/j.indcrop.2026.124532","url":null,"abstract":"","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"10 1","pages":"124532"},"PeriodicalIF":5.9,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149092174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}