Industrial Crops and Products最新文献

{"title":"Integrated volatilomic profiles and chemometrics provide new insights into the aroma differences of volatile compounds in filler tobacco leaves of six grades","authors":"Mingzhu Zhang ,&nbsp;Dongfeng Guo ,&nbsp;Zhiwei Sun ,&nbsp;Guanglong Wu ,&nbsp;Ping Han ,&nbsp;Yaqi Shi ,&nbsp;Rui Zhao ,&nbsp;Lanhua Liu ,&nbsp;Hui Zhang ,&nbsp;Xingjiang Li","doi":"10.1016/j.indcrop.2025.121236","DOIUrl":"10.1016/j.indcrop.2025.121236","url":null,"abstract":"<div><div>As an economically important crop, the quality grade of filler tobacco leaves (FTLs) is a key factor determining their commercial value. To investigate the sources and variations in aroma among different quality grades of filler tobacco leaves (DGFTLs), the volatile components of six DGFTLs were studied using chemometric methods. The results showed that the aroma of high-quality grade FTL exhibited a more balanced aroma profile, while the low-quality grade FTLs displayed a more pronounced pungent odor. The electronic nose (E-nose) analysis using W5S, W1S, W1W, and W2S sensors effectively distinguished the aroma variations of DGFTLs. Notably, 59 common volatile organic compounds (VOCs) were detected across all samples, with (S)-(-)-nicotine, neophytadiene, and aldehydes/ketones being the dominant VOCs in cigar tobacco leaves. By constructing a weighted correlation network model (WCNA), four positively correlated modules and eleven hub VOCs were identified in all FTL varieties. Further multi-stage variable selection revealed that benzeneacetaldehyde, sclareol, farnesylacetone, and 4-methylene-3,5,5-trimethyl-2-cyclohexen-1-one were characteristic markers contributing to the aroma differentiation among the six DGFTLs.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"232 ","pages":"Article 121236"},"PeriodicalIF":5.6,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144098719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the anti-Alzheimer's disease mechanisms of edible medicinal plant Polygonum cuspidatum by integrating network pharmacology and molecular docking","authors":"Xin-Yu Li ,&nbsp;Liang Shen ,&nbsp;Hong-Fang Ji","doi":"10.1016/j.indcrop.2025.121202","DOIUrl":"10.1016/j.indcrop.2025.121202","url":null,"abstract":"<div><div>Alzheimer's disease (AD) is a recognized neurodegenerative disease requiring long-term medication, so it is imperative to develop new drugs that are safe and medicine-food homologous. Previous studies have demonstrated that edible medicinal plant <em>Polygonum cuspidatum</em> possesses substantial neuroprotective properties, while the specific mechanisms through which it operates remain largely unexplored. This study explored the mechanisms by which <em>Polygonum cuspidatum</em> exerts anti-AD effects, utilizing an integration of network pharmacology coupled with molecular docking techniques. Firstly, we screened 456 targets from the 10 active ingredients of <em>Polygonum cuspidatum</em>, with 156 of these targets being relevant to AD. Through topological analysis of these 156 targets, we determined that core targets such as TP53, AKT1, EGFR, IL6, TNF, JUN, IL1B, BCL2, HIF1A, CASP3, MYC, MMP9, PTGS2, CXCL8, and ESR1 play central roles in the against AD of <em>Polygonum cuspidatum</em>. Secondly, it was found that the anti-AD mechanism was closely linked to the PI3K-Akt signaling pathway, a critical component in the regulation of oxidative stress. Thirdly, molecular docking studies indicated that the principal ingredients of <em>Polygonum cuspidatum</em> - quercetin, luteolin, and rhein - exhibit strong binding affinities with the targets MMP9, PTGS2, AKT1, and TNF, establishing them as the main ingredients responsible for its anti-AD properties. Notably, quercetin, luteolin, and rhein demonstrated superior docking affinities compared to other constituents. In summary, these findings suggest that <em>Polygonum cuspidatum</em> may combat AD through various pathways and molecular targets. <em>Polygonum cuspidatum</em> has the potential to alleviate AD symptoms by restoring blood-brain barrier integrity and modulating the PI3K-Akt signaling pathway through its constituents, thereby reducing inflammatory factor activity and mitigating the inflammatory response.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"231 ","pages":"Article 121202"},"PeriodicalIF":5.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144089102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative analysis on methodologies for determining the saturation fiber point in bamboo","authors":"Peng Li ,&nbsp;Xuedong Zheng ,&nbsp;Jian Peng ,&nbsp;Qunying Mou ,&nbsp;Ting Li ,&nbsp;Bangyi Luo ,&nbsp;Lin He ,&nbsp;Xianjun Li ,&nbsp;Xiazhen Li","doi":"10.1016/j.indcrop.2025.121239","DOIUrl":"10.1016/j.indcrop.2025.121239","url":null,"abstract":"<div><div>The stability of dimensional and load-bearing capacity of bamboo, as a green and sustainable engineering material, is critically important during its processing and utilization. Properly managing moisture content through fiber saturation point (FSP) had a significant impact on both bamboo production and its engineering applications. Therefore, the accurate determination of the FSP of bamboo was crucial for practical applications and the effective moisture content control. This study performed a comprehensive analysis on the determination of FSP in bamboo using three methods: LF NMR, mechanical and shrinkage testing. The findings indicated that the FSPs were 23.2 % and 22.3 % as determined by the mechanical method and low-field NMR method, respectively. The shrinkage method produced FSP values of 26.7 %, 26.6 %, and 28.6 % in the longitudinal, tangential and radial directions, respectively. While the three methods yielded comparable experimental outcomes, each demonstrated distinct advantages and limitations in FSP determination. The findings addressed the discrepancies in determining FSPs for bamboo using the three methods and provided essential guidance for the industrial processing and engineering application of bamboo.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"232 ","pages":"Article 121239"},"PeriodicalIF":5.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144098729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-flexible anti-freezing cellulose conductive hydrogel for energy storage and self-powered sensors","authors":"Lijia Jia ,&nbsp;Liying Zhang ,&nbsp;Zhenghan Cai ,&nbsp;Feng Zhu ,&nbsp;Biao Huang ,&nbsp;Qilin Lu","doi":"10.1016/j.indcrop.2025.121222","DOIUrl":"10.1016/j.indcrop.2025.121222","url":null,"abstract":"<div><div>Integrating superior flexibility, conductivity, energy harvesting and broad working temperatures into cellulose hydrogel for deformable energy storage and self-powered sensors has become urgent. Herein, an ultra-flexible anti-freezing conductive versatile hydrogel is developed via synchronously regulating polyvinyl alcohol (PVA) with cellulose nanocrystals (CNCs) and covalent organic frameworks (COFs) to form a multiple hydrogen-bonded ordered network under phytic acid (PA) crosslinking synergy. Introducing COFs into hydrogel network provides continuous protons transport channel while PA generates protons and permanently secures the hierarchical structure of hydrogel, and thus endowing the hydrogel with exceptional proton conductivity (2.46 S/m), ultra-flexibility (tensile strain of 725 %), and outstanding antifreeze conductivity (withstand −40 °C). With these attributes, the hydrogel assembled supercapacitor exhibits stable energy storage capacity (84.7 mF/cm²) and bending resilience (endure bending angles of 180°), whereas the hydrogel-based triboelectric nanogenerator (TENG) presents excellent electrical output performance. Moreover, the designed hydrogel strain sensor demonstrates high sensing sensitivity, enabling stable monitoring of various joint movements and subtle facial expressions. Overall, this study presents a promising approach for designing biomass-based versatile hydrogels with outstanding mechanical performance and energy harvesting and storage functionality for emerging applications in self-powered flexible sensors.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"232 ","pages":"Article 121222"},"PeriodicalIF":5.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144098730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated machine learning and response surface methodology for screening and optimization of high-performance Mg-modified bamboo biochar for phosphorus adsorption in water","authors":"Yuheng Han,&nbsp;Lingzhi Zhu,&nbsp;Xiaoran Li,&nbsp;Dongxu Cui,&nbsp;Jinjin Li,&nbsp;Kehui Cen,&nbsp;Dengyu Chen","doi":"10.1016/j.indcrop.2025.121213","DOIUrl":"10.1016/j.indcrop.2025.121213","url":null,"abstract":"<div><div>Biochar adsorption is an effective method for removing excessive phosphorus from water bodies. In this study, the phosphorus adsorption capacity of biochar was investigated using a machine learning (ML) approach. A dataset consisting of 762 previously published data points from 122 types of biochar was analyzed, with the Random Forest model yielding the best performance (test <em>R</em>² = 0.98, test RMSE = 13.79). The predictions were validated through conducting laboratory experiments on 17 Mg-modified bamboo biochar samples (<em>R</em>² = 0.97, RMSE = 2.8, <em>Q</em>_max = 235.31 mg/g). The optimal preparation conditions were found to be a pyrolysis temperature of 700 °C, pyrolysis time of 3 h, and MgCl₂ concentration of 3 mol/L. X-ray photoelectron spectroscopy confirmed that adsorption performance is positively correlated with Mg content. Kinetic and isotherm tests (Langmuir <em>Q</em>_max = 240.55 mg/g) further supported these findings, providing insights for the preparation of high-performance Mg-modified bamboo biochar.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"231 ","pages":"Article 121213"},"PeriodicalIF":5.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144089119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing tobacco (Nicotiana tabacum L.) carbon metabolism regulation via topping: A study based on multi-omics","authors":"Nuo Shi ,&nbsp;Meng Jia ,&nbsp;Xuanquan Zhu ,&nbsp;Qi Luo ,&nbsp;Yuxiang Bai ,&nbsp;Na Wang ,&nbsp;Yu Du ,&nbsp;Dingchun Zi ,&nbsp;Limei Zhang ,&nbsp;Tikun Zhang ,&nbsp;Ge Wang ,&nbsp;Peng Zhou","doi":"10.1016/j.indcrop.2025.121196","DOIUrl":"10.1016/j.indcrop.2025.121196","url":null,"abstract":"<div><div>Topping eliminates the apical dominance of tobacco, alters the structure of tobacco leaves, and regulates the chemical composition of tobacco leaves. This study investigated how different topping periods affect photosynthetic carbon metabolism and related regulatory mechanisms in tobacco leaves through integrated physiological, proteomic, and metabolomic analyses. The results indicated that topping could effectively promote the broadening of leaves and the increase of leaf area, and significantly enhance the photosynthetic rate of leaves. Furthermore, topping promotes the Calvin cycle and sucrose metabolism in photosynthetic organisms, increasing starch content and reducing sucrose content. Overall, topping promotes photosynthetic carbon metabolism. These results not only contribute a theoretical basis for the response mechanism and physiological changes in tobacco as the target products under different topping periods but also providing theoretical support for optimizing tobacco planting practices.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"231 ","pages":"Article 121196"},"PeriodicalIF":5.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144089117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of ecological shading on the quality of Lushan Yunwu summer tea","authors":"Yanan Liu ,&nbsp;Xing Xu ,&nbsp;Yan Chen ,&nbsp;Changshuai Yu ,&nbsp;Wenxin Liu ,&nbsp;Yangling Zhang ,&nbsp;Jigang Huang ,&nbsp;Hongfei Wei ,&nbsp;Xinfeng Jiang ,&nbsp;Yinxiang Gao","doi":"10.1016/j.indcrop.2025.121197","DOIUrl":"10.1016/j.indcrop.2025.121197","url":null,"abstract":"<div><div>Lushan Yunwu Tea (LSYWT) is one of China’s renowned green teas. As an approach to sustainable ecological management, ecological shading has been scarcely studied in terms of its influence on the quality of LSYWT. In this study, tea trees that are grown in monoculture (CK), with partial shading (ES1), and with full shading (ES2), were selected to investigate how ecological shading affects flavor substances and aroma components of tea. Our findings indicate that catechins, caffeine, and theobromine were significantly increased under ES1 and ES2, and the total amounts of umami and sweet amino acids, as well as bitter amino acids, were highest in ES1. The taste activity value (TAV) of aspartic acid, glutamic acid, and theanine, which are related to umami, were highest in ES1, while the taste activity value of arginine, associated with astringency, was highest in CK. A total of 85 volatile compounds were identified across CK, ES1, and ES2. The results indicated that cis-jasmone, indole and cis-3-hexenyl hexanoate were characteristic aroma compounds of ES1. This study demonstrates that fresh tea leaves cultivated under ecological shading (ES1) conditions can be processed into LSYWT exhibiting characteristic chestnut-like aroma and floral notes, accompanied by significantly reduced bitterness.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"232 ","pages":"Article 121197"},"PeriodicalIF":5.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144098831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental and biological factors influence flavonoid and saponin contents in Ziziphi Spinosae Semen in the taihang mountains","authors":"Shulei He,&nbsp;Mengyu Li,&nbsp;Yanmei Chen,&nbsp;Qianyuan Liu","doi":"10.1016/j.indcrop.2025.121216","DOIUrl":"10.1016/j.indcrop.2025.121216","url":null,"abstract":"<div><div>Geographic variation is vital for regulating secondary metabolite synthesis in medicinal plants. In this study, the influence of ecological and biological factors on the accumulation of major medicinal ingredients in <em>Ziziphus jujuba</em> var. <em>spinosa</em> (Z. <em>jujuba</em>) seeds, commonly known as <em>Ziziphi spinosae semen</em> (ZSS), across 38 natural populations in the Taihang Mountains of China, was investigated. Among the four medicinal ingredients (spinosin, 6‴-feruloyl spinosin, jujuboside A, and jujuboside B), jujuboside B exhibited the greatest variation across regions. The combined effects of environmental and biological factors explained 47.60 % of the chemical variation in ZSS, with climatic and soil factors contributing more substantially than biological traits. Jujuboside B, indicating high environmental and biological sensitivity, was significantly negatively correlated with the growing season effective accumulated temperature, precipitation, long-term average annual temperature, soil clay content, soil nitrogen (SN), total organic carbon (TOC), leaf nitrogen content, and fresh fruit edible ratio (<em>P</em> &lt; 0.05). Jujuboside B was positively correlated with longitude, latitude, soil total phosphorus (TP), and the nutlet rate (<em>P</em> &lt; 0.05). Jujuboside A was significantly negatively correlated with the relief degree of the land surface (RDLS), SN, and leaf water content (LW) (<em>P</em> &lt; 0.05). The spinosin content was negatively correlated with SN, TOC, fruit rate, and LW (<em>P</em> &lt; 0.05), whereas 6‴-feruloyl spinosin was negatively associated with the soil electrical conductivity and LW (<em>P</em> &lt; 0.05). This study revealed that climate factors are crucial for regulating the accumulation of medicinal ingredients in ZSS. Furthermore, lower soil nutrients (such as low TOC, SN, and high sand contents) promoted the accumulation of active constituents. This study deepens our understanding of the ecological mechanisms underlying the variation in ZSS chemical composition and provides a scientific foundation for its ecological cultivation and quality improvement.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"231 ","pages":"Article 121216"},"PeriodicalIF":5.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144106621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trichoderma crassum secrets N, N′-diacetylchitobiose and indole-3-acetic acid to enhance the fibrous root formation in P. heterophylla","authors":"Lingling Wang ,&nbsp;Yanping Gao ,&nbsp;Lang Qin ,&nbsp;Jiayue Ran ,&nbsp;Zan Li ,&nbsp;Xianyan Xie ,&nbsp;Yangjun Tan ,&nbsp;Tao Zhou ,&nbsp;Qing-Song Yuan","doi":"10.1016/j.indcrop.2025.121227","DOIUrl":"10.1016/j.indcrop.2025.121227","url":null,"abstract":"<div><div><em>Pseudostellaria heterophylla</em> (Miq.) Pax ex Pax et Hoffm., commonly called Taizishen, is a highly esteemed and edible root-usage traditional Chinese medicine. Enhancing its fibrous root formation is imperative for augmenting yield and quality. This study employed metabolome and transcriptome profiling to investigate the plant growth promotion mechanism of <em>Trichoderma crassum</em> strain WMM-1–7 on <em>P. heterophylla</em>. Strain WMM-1–7 significantly enhanced the growth of <em>P. heterophylla</em>, facilitating the formation of fibrous roots. Strain WMM-1–7 significantly induced the expression of auxin response genes (AUX/IAA, GH3) and other auxin-related plant hormones response genes (GID1, PYR/PYL) in plant hormone signal transduction pathway, as well as of the genes (B-ARR, BSK, TCH4) involving cell division and enlargement. Meanwhile, the transcription factor C3H is also intricately engaged in the process of cell wall re-architecture. Further, strain WMM-1–7 reshaped the metabolites profiling cocultured with <em>P. heterophylla</em>. These co-induced metabolites have nine main categories: organic acids and their derivatives, organ heterocyclic compounds, and lipids and lipid-like molecules. Additionally, correlation analysis showed that 34 metabolites were significantly associated with the number of fibrous roots. Finally, both indole-3-acetic acid and N,N′-diacetylchitobiose strongly promote fibrous root formation and enhance the plant height in <em>P. heterophylla</em>. These results demonstrate that strain WMM-1–7 can produce multiple metabolites to promote the formation and development of the fibrous root of <em>P. heterophylla</em>, which provides new insights for regulating plant growth and new elicitor.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"231 ","pages":"Article 121227"},"PeriodicalIF":5.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144106619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel beta-glucosidase gene from Angelica dahurica and insights into the metabolic pathway of scopoletin","authors":"Lei Yuan ,&nbsp;Yao Zhang ,&nbsp;Siqing Peng ,&nbsp;Huapeipei Huang ,&nbsp;Jing Zhang ,&nbsp;Baoyang Wei ,&nbsp;Xiangyang Lu ,&nbsp;Yun Tian ,&nbsp;Xiaohong Zhong ,&nbsp;Yuedong He","doi":"10.1016/j.indcrop.2025.121219","DOIUrl":"10.1016/j.indcrop.2025.121219","url":null,"abstract":"<div><div><em>Angelica dahurica</em> is a traditional Chinese medicinal product with anti-inflammatory and analgesic effects, which has received substantial attention from researchers in recent years. Scopoletin, which is an important factor involved in plant defense, and other coumarin compounds are the main active ingredients in <em>A. dahurica</em>. <em>β</em>-glucosidases (BGLUs), which hydrolyze the <em>β</em>-D-glycosidic bond, are involved in physiological processes such as plant growth, development, and defense. However, the functional role of BGLUs in <em>A. dahurica</em> remains unclear. In this study, three BGLUs were isolated and purified from <em>A. dahurica</em> (<em>AdBGLUs</em>) through transcriptomic analysis. <em>AdBGLU1</em> was found to catalyze the generation of scopoletin from scopolin <em>in vitro</em>. It belongs to the GH1 subfamily of BGLUs that contains 547 amino acid residues and possesses the motifs required to catalyze the hydrolysis of the glycosidic bond. Substrate promiscuity testing showed that <em>AdBGLU1</em> could convert skimmin, isoscopolin and tectoridin to umbelliferone, isoscopletin and tectorigenin, respectively. Molecular docking of <em>AdBGLU1</em> with scopolin, skimmin, and tectoridin revealed key amino acid residues involved in their binding, such as GLN33, GLU183, and GLU453. <em>Agrobacterium</em>-mediated transient transformation system and RT-qPCR analysis showed that <em>AdBGLU1</em> played a crucial role in the synthesis of scopoletin. This study focused on the BGLUs of <em>A. dahurica</em> and found its novel role in the biosynthesis of scopoletin. In addition, we speculated the mechanism through which scopoletin contributes to defense processes in <em>A. dahurica</em>. Altogether, the findings of this study provide valuable insights into the role of <em>AdBGLU1</em> in the biosynthesis of scopoletin and its regulatory mechanisms.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"231 ","pages":"Article 121219"},"PeriodicalIF":5.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144089116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}