Analysis of mRNA-miRNA networks in soybean lipid metabolism and the regulatory role of gma-miRNA396b based on transcriptomics

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products Pub Date : 2025-10-10 DOI:10.1016/j.indcrop.2025.121890

Aijing Zhang , Feiwu Liu , Yue Li , Wenxuan Pi , Huijing Liu , Shuying Liu , Jian Wei , Xiaoyu Lu , Dan Yao

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Abstract

Soybean (Glycine max (Linn.) Merr.) is a globally important crop. It is extensively utilized in food processing, feed production, and various industrial applications. miRNAs are involved in various plant biological processes, particularly in growth, development, differentiation, and biotic and abiotic stress responses. However, their involvement in lipid synthesis in soybean has rarely been reported. In this study, the soybean cultivar ‘JN18’ and its low linolenic acid mutant ‘MT72’ were used as experimental material at R4 (pod filling stage), R5 (initiation of seed formation), and R6 (peak of seed formation) for whole-transcriptome sequencing. miRNAs related to lipid synthesis were identified and miRNA-mRNA network was constructed. Overexpression of gma-miR396b leaded to a significant reduction in oleic acid content and total hydrolyzed amino acids in the seeds. The interaction between gma-miR396b and GmLACS1 was validated by rapid amplification of cDNA ends mediated and β-glucuronidase (GUS) histochemical staining assays. Our results provide a new perspective on the molecular network of soybean lipid metabolism.

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基于转录组学的大豆脂质代谢mRNA-miRNA网络及gma-miRNA396b的调控作用分析

大豆（甘氨酸max（亚麻））小麦是一种全球重要作物。广泛应用于食品加工、饲料生产和各种工业应用。mirna参与多种植物生物学过程，特别是在生长、发育、分化以及生物和非生物胁迫反应中。然而，它们参与大豆脂质合成的报道很少。本研究以大豆品种“JN18”及其低亚麻酸突变体“MT72”为实验材料，分别在R4（荚果灌浆期）、R5（种子形成起始期）和R6（种子形成高峰期）进行全转录组测序。鉴定与脂质合成相关的mirna，构建miRNA-mRNA网络。过表达gma-miR396b导致种子中油酸含量和总水解氨基酸含量显著降低。gma-miR396b与GmLACS1的相互作用通过cDNA末端介导的快速扩增和β-葡萄糖醛酸酶（GUS）组织化学染色验证。本研究结果为研究大豆脂质代谢的分子网络提供了新的视角。

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来源期刊

Industrial Crops and Products 农林科学-农业工程

CiteScore

9.50

自引率

8.50%

发文量

1518

审稿时长

43 days

期刊介绍： Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.