Insect Molecular Biology最新文献

{"title":"RNA activation in insects: From emerging evidence to molecular applications.","authors":"Sassan Asgari","doi":"10.1111/imb.70058","DOIUrl":"https://doi.org/10.1111/imb.70058","url":null,"abstract":"<p><p>RNA interference is commonly used as a reverse genetics tool to investigate gene function. In insects, this is achieved by introducing double-stranded RNA or synthetic short-interfering RNAs (siRNAs). However, RNA activation (RNAa) is less frequently utilised in these studies. RNAa can be achieved by targeting a gene's promoter with antisense RNA, which triggers endogenous transcription. Unlike the siRNA mechanism, which is well characterised, the RNAa mechanism is less well known. In the literature, 'RNAa' has been adopted to describe diverse small RNA-mediated upregulation mechanisms that may not necessarily function through the canonical RNAa mechanism described in mammals, which involves interaction of the small RNA with the promoter region. Although the outcomes of RNAi and RNAa differ, some components are shared between their pathways, such as Argonaute proteins. In this minireview, RNAa, its canonical and non-canonical mechanisms, and applications in insect research are examined with the aim of encouraging its use as a tool in Insect Molecular Biology.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148420911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of catalpol on glycolipid homeostasis and lifespan of Drosophila melanogaster","authors":"Yan Li,&nbsp;Yuxin Ge,&nbsp;Yanlin Luo,&nbsp;Sunhong Zhu,&nbsp;Min Zhou,&nbsp;Yongsheng Chang,&nbsp;Bin Tang","doi":"10.1111/imb.70038","DOIUrl":"https://doi.org/10.1111/imb.70038","url":null,"abstract":"<p>Glycolipid metabolism is a core regulatory link in the energy homeostasis and physiological functions of insects, directly affecting their survival, development, reproduction and environmental adaptability. Catalpol, the main active component of <i>Rehmannia glutinosa</i>, possesses multiple pharmacological activities, such as antioxidation, anti-inflammation and regulation of glycolipid metabolism. However, the specific regulatory mechanism of its effect on insect metabolism remains unclear. In this study, <i>Drosophila melanogaster</i> was used as the model organism to systematically explore the effects of catalpol on glycolipid metabolism, growth and development, motor ability and lifespan of female adults, as well as its underlying molecular mechanism. At the molecular level, catalpol significantly enhances glucose catabolism, as demonstrated by increased activity of both soluble and membrane-bound trehalase and upregulation of the <i>Tret1-1</i> gene. Concurrently, it dynamically regulated lipid metabolism, significantly increasing the content of free fatty acids and triglycerides. This finding, coupled with the upregulation of key lipolytic genes such as <i>hormone-sensitive lipase</i> (<i>HSL</i>), indicates accelerated fat turnover. These metabolic modulations translated into significant phenotypic improvements. Catalpol treatment promoted insect growth and development, as evidenced by increased body weight, abdominal size and wing area. Furthermore, it enhanced their motor ability, shown by improved climbing, and extended their average lifespan and survival rate, suggesting a delay in ageing. In conclusion, this study elucidates a novel regulatory model, wherein catalpol dynamically modulates both glucose and lipid metabolism, leading to improved growth, enhanced motor function and extended lifespan in <i>D. melanogaster</i>. Our findings provide initial insights into the intricate mechanism-phenotype connection and provide compelling experimental evidence for catalpol's potential as an insect physiological regulator. This research not only offers new perspectives on insect energy homeostasis but also lays a foundation for its application in agricultural pest control and beneficial insect breeding.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 4","pages":"386-398"},"PeriodicalIF":3.3,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148522931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging experimental and bioinformatic approaches in RNA interference-based pest control research","authors":"Doga Cedden","doi":"10.1111/imb.70040","DOIUrl":"10.1111/imb.70040","url":null,"abstract":"<p>RNA interference (RNAi) has emerged as a promising strategy for species-specific and environmentally friendly pest control, offering an alternative to conventional chemical insecticides that are increasingly constrained by resistance development and ecological concerns. RNAi-based approaches involve oral delivery of double-stranded RNA (dsRNA), which is processed into RNA-induced silencing complex (RISC)-bound small interfering RNA (siRNA) to silence essential genes of pests. This review synthesizes recent advances in experimental and bioinformatic methodologies that are facilitating and enhancing RNAi research in insect pest management. Particular emphasis is placed on molecular validation techniques that move beyond phenotype-based bioassays, including RISC-bound small RNA sequencing to resolve dsRNA processing and guide strand selection, RNA degradomics to map siRNA-mediated transcript cleavage events and transcriptomic and proteomic profiling to characterize genome-wide responses and compensatory effects. In parallel, dsRNA visualization methods provide mechanistic insight into uptake, intracellular trafficking and degradation dynamics, clarifying barriers that distinguish responsive from recalcitrant species. Complementing these experimental developments, emerging computational platforms enable insect-optimized target selection, dsRNA design and environmentally informed off-target prediction. Together, these innovations support a transition toward more predictive and mechanistically grounded RNAi-based pest control applications. The integration of high-resolution molecular tools with specialized bioinformatic pipelines is expected to enhance efficacy, safety and reproducibility, advancing RNAi-based pest control toward practical and scalable agricultural deployment.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 4","pages":"364-375"},"PeriodicalIF":3.3,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imb.70040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147627725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"2-Oxocarboxylic acid metabolic pathway fuels mitochondrial ATP synthesis to power oviposition in Bactrocera dorsalis","authors":"Long Ye,&nbsp;Qiaoling Zhang,&nbsp;Jingwei Yang,&nbsp;Daifeng Cheng","doi":"10.1111/imb.70033","DOIUrl":"10.1111/imb.70033","url":null,"abstract":"<p>For many insects, oviposition is an energy-intensive behaviour. However, the metabolic mechanisms powering this process are poorly understood for many species. Here, we investigated the link between energy metabolism and oviposition in <i>Bactrocera dorsalis</i>. The results showed that maturation of the ovipositor was accompanied by a significant increase in mitochondrial number. Transcriptomic analysis identified the 2-oxocarboxylic acid metabolic pathway as the most significantly up-regulated during development. Six core genes (citrate synthase [CS], aconitase [ACO], dehydrogenase 3 [IDH3]α, IDH3β, IDH3γ, glutamic-oxaloacetic transaminase 1 [GOT1]) within this pathway showed continuous expression increases, peaking at the oviposition stage, and were specifically enriched in ovaries and mature ovipositors. RNAi-mediated co-silencing of IDH3 subunits or individual knockdown of CS, ACO or GOT1 significantly reduced both egg deposition and ovipositor ATP levels, whereas individual IDH3 subunit knockdowns showed no significant effect. The ATP synthase inhibitor oligomycin A similarly induced a significant reduction in fecundity and disrupted ovipositor ATP homeostasis. Our study demonstrates that <i>B. dorsalis</i> oviposition is critically dependent on a tissue-specific 2-oxocarboxylic acid metabolic pathway that fuels mitochondrial ATP synthesis in the ovipositor. This establishes a direct functional link between core energy metabolism and reproductive behaviour, revealing novel potential targets for pest control.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 4","pages":"376-385"},"PeriodicalIF":3.3,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imb.70033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147372497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological role and application potential of HcCAT2 in multi-host adaptability of Hyphantria cunea revealed by RNA interference","authors":"Xue Yan,&nbsp;Laguoma Yi,&nbsp;Lin Zheng,&nbsp;Shanchun Yan,&nbsp;Dun Jiang","doi":"10.1111/imb.70042","DOIUrl":"10.1111/imb.70042","url":null,"abstract":"<p>The antioxidant defence system is crucial for herbivorous insects to adapt to various host plants. This study focused on <i>catalase 2</i> gene (<i>HcCAT2</i>) to investigate the antioxidant mechanisms that underlie the ability of <i>Hyphantria cunea</i> to adapt to a wide range of hosts and to develop a disruptor for its polyphagous behaviour. Results indicated that, compared to larvae fed on the highly preferred host plant <i>Morus alba</i>, <i>HcCAT2</i> expression increased 33.19- to 47.25-fold in larvae reared on <i>Betula platyphylla</i> and <i>Tilia amurensis</i> with moderate and low preference, respectively. Silencing <i>HcCAT2</i> consistently reduced larval body weight and downregulated growth-related genes (e.g., <i>Cyclin A2</i> and <i>Decapentaplegic</i>) across all host plant groups. Moreover, <i>HcCAT2</i> silencing significantly downregulated key glycolytic genes (<i>Hexokinase</i> and <i>Pyruvate kinase</i>), the tricarboxylic acid cycle gene (<i>Isocitrate Dehydrogenase 2</i>) and lipid metabolism genes (e.g., <i>Acetyl-CoA Carboxylase</i>) in larvae fed on all three host plants. The redox response in larvae was complex, as <i>HcCAT2</i> silencing led to a marked downregulation of negative regulators of oxidative damage and key ROS-producing genes. The nucleic acid pesticide CS-ds<i>HcCAT2</i>, designed to target <i>HcCAT2</i> expression, demonstrated potent silencing efficacy. Treatment with CS-ds<i>HcCAT2</i> suppressed larval body weight on all host plants. Collectively, the <i>HcCAT2</i>-mediated antioxidant defence system is critical to the host plant adaptation of <i>H. cunea</i>, and CS-ds<i>HcCAT2</i>, by inhibiting <i>HcCAT2</i> expression, holds promise as an effective agent to disrupt the polyphagous behaviour of <i>H. cunea</i>.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 4","pages":"463-475"},"PeriodicalIF":3.3,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147948292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal, not insect: Revisiting a misidentified Bemisia tabaci protein interacting with a begomovirus coat protein","authors":"Jesús Navas-Castillo,&nbsp;Elvira Fiallo-Olivé","doi":"10.1111/imb.70037","DOIUrl":"10.1111/imb.70037","url":null,"abstract":"<p>Understanding interactions between viral proteins and those of their biological vectors is essential for elucidating transmission mechanisms and identifying potential targets for disease control. Insect vectors, among the most common and significant transmitters of animal and plant viruses, play a crucial role in viral transmission dynamics. Such interactions can influence virus transmission efficiency and provide insights into how viral diseases spread within ecosystems. Accurate identification of the insect proteins involved is vital, as misidentifications can lead to erroneous conclusions that misguide subsequent research and control strategies. In the case of begomoviruses (<i>Geminiviridae</i>: <i>Begomovirus</i>), a protein previously identified as insect-derived by Ohnesorge and Bejarano (2009) is shown here, based on the analyses presented in this Commentary, to be of likely fungal origin. This misidentification, based on yeast two-hybrid and pull-down experiments, has been repeatedly cited in the literature, perpetuating an incorrect conclusion. Establishing the correct identity of this protein is essential for guiding future research on insect-virus interactions.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 4","pages":"361-363"},"PeriodicalIF":3.3,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imb.70037","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147468017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-133 activates notch signalling by repressing Synaptobrevin to control ligand localisation in Drosophila","authors":"Cheng-Hsien Lee,&nbsp;Yun-Chien Hung,&nbsp;Jian-Chiuan Li,&nbsp;Chun-Hong Chen,&nbsp;Yu-Chen Tsai","doi":"10.1111/imb.70036","DOIUrl":"10.1111/imb.70036","url":null,"abstract":"<p>Precise regulation of Notch signalling is essential for proper eye development in <i>Drosophila</i>. Although the core components of Notch signalling are well characterised, the mechanisms modulating ligand activity remain less understood. Here, we identified microRNA <i>miR-133</i> as a positive regulator of Notch signalling in a <i>Drosophila</i> eye model. In a Notch-sensitised background, <i>miR-133</i> overexpression rescued the small-eye phenotype induced by <i>fringe</i> (<i>fng</i>), while <i>miR-133</i> knockdown exacerbated this defect. <i>miR-133</i> directly targets <i>Synaptobrevin</i> (<i>Syb</i>), a vesicle-associated SNARE protein, through a conserved site in its 3′UTR. Loss of <i>Syb</i> phenocopied <i>miR-133</i> overexpression, whereas <i>Syb</i> overexpression enhanced Notch-related defects. <i>miR-133</i> and <i>Syb</i> exhibited complementary expression patterns, and both <i>miR-133</i> overexpression and <i>Syb</i> knockdown induced non-autonomous activation of Notch targets and accumulation of Delta and Serrate ligands at the plasma membrane. Furthermore, we show that the E3 ligase Godzilla (Gzl) and Rab11-dependent recycling are essential for Notch activation. Together, our findings revealed a <i>miR-133/Syb/gzl/Rab11</i> regulatory axis that enhances Notch signalling by modulating ligand trafficking and distribution during development.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 4","pages":"417-433"},"PeriodicalIF":3.3,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147520961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nuclear receptor genes HR3 and βFTZ-F1 are involved in moulting development, pupation and eclosion in Hyphantria cunea (Drury)","authors":"Jingqing Chu,&nbsp;Jiawen Zhang,&nbsp;Lili Sun,&nbsp;Chuanwang Cao","doi":"10.1111/imb.70043","DOIUrl":"10.1111/imb.70043","url":null,"abstract":"<p>Nuclear receptor genes play crucial roles in insect moulting. These genes, found in all insects, produce important proteins that control the expression of other genes, which in turn regulate the moulting process in larvae. This study investigated the roles of the nuclear receptor genes <i>HcHR3</i> and <i>HcβFTZ-F1 in</i> the moulting development, pupation and eclosion of <i>Hyphantria cunea</i>. The mRNA expression of the two genes was detected at all developmental stages and in various adult tissues. A novel pET28a (+)-HT115 (DE3) RNase III-system was successfully constructed to efficiently express <i>HcHR3</i>-dsRNA and <i>HcβFTZ-F1</i>-dsRNA in bacteria. To explore the physiological functions of these genes, they were knocked down in larvae and pupae using the dsRNA extracted from bacteria, resulting in a decrease in the expression levels of <i>HcHR3</i> and <i>HcβFTZ-F1</i> by 13.70% and 99.01%, respectively. Moulting defects were observed in <i>H. cunea</i> larvae following <i>HcHR3</i> and <i>HcβFTZ-F1</i> silencing, with a phenotypic percentage ranging from 33.40% to 45.22%. Additionally, knockdown of these two genes in 5th instar larvae decreased weight, food intake and the survival rate, resulting in abnormal development during the larval–pupal transition. RNAi-mediated knockdown of <i>HcHR3</i> and <i>HcβFTZ-F1</i> prevented the eclosion of the pupae, with malformation rates ranging from 75.67% to 80.12%, and epidermal tanning defects were observed in pupae and adults. Our findings demonstrate that <i>HcHR3</i> and <i>HcβFTZ-F1</i> are indispensable for normal postembryonic development and provide validated molecular targets for RNAi-based pest management and insight into the outbreak mechanisms of <i>H. cunea</i>.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 4","pages":"448-462"},"PeriodicalIF":3.3,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148042086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The pigment-dispersing factor receptor (PDFR) gene is involved in circadian rhythm and moulting in Hyphantria cunea.","authors":"Xue Li, Ge Zhao, Rongxia Zhao, Zhengxuan Wang, Lili Sun, Chuanwang Cao","doi":"10.1111/imb.70057","DOIUrl":"https://doi.org/10.1111/imb.70057","url":null,"abstract":"<p><p>The pigment-dispersing factor neuropeptide receptor (PDFR) has a key role in establishing and maintaining the circadian rhythm, such as reproductive behaviour and ecdysone biosynthesis in numerous insect species. However, the functions of the PDFR gene are still unknown in Hyphantria cunea. In this study, the expression level of the HcPDFR gene during the 6th instar stage of larvae is higher than those in the other developmental stages. The HcPDFR gene is highly expressed in the testis compared to the other tissues. Additionally, a significant rhythmic expression of HcPDFR was observed in the head under the photoperiod of 16L:8D and 12L:12D, while it showed no rhythmicity under 24L:0D. The physiological function of the PDFR gene was identified by RNA interference (RNAi) technology. After the HcPDFR was silenced, the timing of moulting of the 5th instar larvae was disrupted, as the peak time of moulting moved forward under the photoperiod of 16L:8D and 12L:12D, and the moulting time completely lost rhythm under 24L:0D. Furthermore, interfering with HcPDFR leads to a decrease in pupation rate and an increase in the mortality rate. These findings suggested that targeting the HcPDFR could be a potential strategy to control the damage caused by the leaf-eating pest, H. cunea.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148404645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockout of Ku70 and Ku80 elevates homology-directed repair efficiency in Plutella xylostella","authors":"Faisal Munir,&nbsp;Uroosa Zaheer,&nbsp;Muhammad Asad,&nbsp;Guang Yang","doi":"10.1111/imb.70039","DOIUrl":"10.1111/imb.70039","url":null,"abstract":"<p>The suppression of Ku70 and Ku80 has been verified to increase homology-directed repair (HDR) efficiency in fruit fly, silkworm and mosquito, but not in other insects. In this study, <i>PxKu70</i> and <i>PxKu80</i> were identified from the <i>Plutella xylostella</i> genome. Domain analysis revealed that PxKu70 contained three conserved domains: Ku N, Ku and Ku C, while PxKu80 comprised the Ku N, Ku and Ku PK bind domains. Phylogenetic analysis and multiple-sequence alignment indicated strong conservation of both proteins among lepidopteran insects. RT-qPCR analysis showed that <i>PxKu70</i> and <i>PxKu80</i> were highly expressed in adult stages, particularly in reproductive tissues such as the ovary and testis, suggesting their role in maintaining genomic stability during gametogenesis. Two homozygous knockout lines (Δ<i>PxKu70</i> and Δ<i>PxKu80</i>) were successfully generated through CRISPR/Cas9-mediated genome editing. These knockout lines remained viable and fertile without observable fitness effects. A donor construct carrying an <i>EGFP</i> cassette designed for insertion at the <i>PxKmo</i> locus was generated to assess HDR-mediated integration. The HDR insertion rate was significantly elevated in both knockout lines compared with the wild-type. These findings demonstrate that suppression of either <i>PxKu70</i> or <i>PxKu80</i> can enhance HDR in <i>P. xylostella</i>, offering an effective approach for precise genome editing in lepidopteran species.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 4","pages":"434-447"},"PeriodicalIF":3.3,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147689819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}