{"title":"PBX and Dfd cooperatively regulate stage-specific expression of 30 K protein BmLP1 in Bombyx mori.","authors":"Qingqing Linghu, Haoyun Li, Jiahui Wan, Xiaolu Zhang, Jingjing Huang, Zhiqing Li, Yuying Wang, Jianhua Xia, Zhaoming Dong, Ying Lin, Ping Zhao, Yan Zhang","doi":"10.1111/imb.12998","DOIUrl":"https://doi.org/10.1111/imb.12998","url":null,"abstract":"<p><p>Nutrient accumulation is essential for insect metamorphosis. As a group of important nutrient-storage proteins, forty-six 30 K proteins (30KPs), including BmLP1-BmLP46, have been identified in the silkworm, Bombyx mori. Most 30KPs are synthesised in the last instar larvae, and the stage-specific expression of 30KPs is believed to be regulated by juvenile hormone (JH)-dependent pathways; however, the specific regulatory mechanism remains unclear. In this study, we found that a 30KP gene Bmlp1 was expressed after Day 3 of the fifth instar, and its expression was down-regulated by JH analogue. We also identified a cis-response element (CRE) on the promoter of Bmlp1. Dfd was determined to bind to this CRE adjacent to another CRE that serves as a binding site for PBX. Dfd is a HOX transcription factor found to exhibit an expression pattern similar to that of PBX. The interaction between PBX and Dfd was confirmed using bimolecular fluorescence complementation and GST pull-down experiments. The expression of Bmlp1 was down-regulated when PBX and Dfd were overexpressed in BmN cells, whereas it was up-regulated when PBX and Dfd were knocked down in BmN cells. Our data show that the transcription factor Dfd, and the cofactor PBX, synergistically regulate the transcription of Bmlp1 in B. mori. This study provides a reference for an in-depth understanding of the regulation of insect development mediated by JH.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mechanism of Ca<sup>2+</sup> in regulating pupation defects of Bombyx mori after exposure to chlorantraniliprole.","authors":"Jialu Cheng, Haoyi Gu, Hongbin Zou, Xiaoxia Zhang, Peiling Peng, Xueling Qin, Bing Li","doi":"10.1111/imb.12999","DOIUrl":"https://doi.org/10.1111/imb.12999","url":null,"abstract":"<p><p>Chlorantraniliprole (CAP) is a novel amide insecticide widely used in agriculture. Trace residues of CAP in the environment pose a threat to the development and metamorphosis of silkworm (Bombyx mori). However, the mechanisms by which CAP exposure disrupts insect metamorphosis remain poorly understood. This study investigated the levels of intracellular Ca<sup>2+</sup> and 20-hydroxyecdysone (20E) following exposure to low concentrations of CAP. The results revealed that CAP exposure both directly caused increased Ca<sup>2+</sup> levels and indirectly promoted an increase in Ca<sup>2+</sup> levels by inducing an elevation in 20E levels. Furthermore, increased Ca<sup>2+</sup> level inhibited the expression of Ftz-f1, leading to abnormal pupation. Our study reveals, for the first time, the sublethal effects of CAP mediated by the Ca<sup>2+</sup>-Ftz-f1 axis. The findings herein provide a reference for evaluating the safety of environmental residues of amide pesticides on insects.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mike Darrington, Jason Solocinski, Sophia K. Zhou, Melise C. Lecheta, Subba Reddy Palli, Yolanda H. Chen, Nicholas M. Teets
{"title":"Environmental factors affecting RNAi efficacy: Temperature but not plant cultivar influences Colorado potato beetle's response to insecticidal dsRNA","authors":"Mike Darrington, Jason Solocinski, Sophia K. Zhou, Melise C. Lecheta, Subba Reddy Palli, Yolanda H. Chen, Nicholas M. Teets","doi":"10.1111/imb.12996","DOIUrl":"10.1111/imb.12996","url":null,"abstract":"<p>Environmental RNAi (eRNAi) is a recent innovation in insect pest control, and comprehensive risk assessment is needed to ensure the environmental safety and longevity of this technology. As eRNAi relies on the insect's cellular machinery for its mode of action, environmentally mediated plasticity in the activity of cellular processes required for RNAi could influence efficacy and the development of resistance. Here, we investigated the extent to which plant cultivar and temperature influence the efficacy of insecticidal double-stranded RNA (dsRNA) targeting <i>actin</i> in larvae of the Colorado potato beetle, <i>Leptinotarsa decemlineata</i> Say (Coleoptera: Chrysomelidae). Potato cultivar did not significantly affect survival or gene silencing in dsRNA-treated larvae, indicating that efficacy is consistent across potato varieties, at least under laboratory conditions. Temperature did influence RNAi efficacy, with both gene silencing and mortality being reduced when dsRNA treatment occurred at lower temperatures. After 3 days of feeding with dsRNA, gene silencing occurred at all temperatures, but knockdown efficiency was 62% at 30°C and 35% at 18°C. eRNAi efficacy at different temperatures was not related to transcript levels of core RNAi genes, indicating that other mechanisms are responsible for the observed effects. Overall, these results indicate that environmental conditions can influence the efficacy of insecticidal eRNAi and may affect the rate at which insects develop resistance to these technologies.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"34 4","pages":"581-592"},"PeriodicalIF":2.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imb.12996","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dual oxidase is essential for moulting, hatching and feeding in the brown planthopper.","authors":"Jinjin Ren, Shuai Tao, Xu Cheng, Yanyuan Bao","doi":"10.1111/imb.12995","DOIUrl":"https://doi.org/10.1111/imb.12995","url":null,"abstract":"<p><p>Dual oxidase (Duox) is well-known for its role in immunity and tyrosine cross-linking activity across various biological processes from mammals to holometabolous insects. Nevertheless, its function in hemimetabolous insects remains poorly understood. In this study, we explored the physiological roles of the Duox gene in a hemimetabolous insect, the brown planthopper, one of the most devastating rice pests. A comprehensive analysis of the spatiotemporal expression pattern of the Duox gene was conducted. RNA interference (RNAi)-mediated silencing of the Duox gene led to moulting defects in nymphs, wing abnormalities and impaired feeding in adults and reduced hatchability in eggs. Additionally, Duox knockdown significantly reduced hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) levels in premoulting nymphs and female ovaries. These findings highlight the indispensable role of Duox in moulting, hatching, wing expansion and feeding behaviours in the brown planthopper, shedding light on the relationship between H<sub>2</sub>O<sub>2</sub> production and cuticle structural stability.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"HcTRET1 is critical for epidermal chitin synthesis in Hyphantria cunea.","authors":"Diankuan Liu, Chuanshan Zou, Shengyu Zhang, Ze Wang, Jinxin Yu, Yuyao Nan, Zixin Dong","doi":"10.1111/imb.12994","DOIUrl":"https://doi.org/10.1111/imb.12994","url":null,"abstract":"<p><p>In insects, trehalose is critical for growth and development, as well as environmental stress response, which is mainly transported by trehalose transporters (TRETs). Over nearly two decades, the physiological functions of TRETs in insect growth, development, reproduction and environmental stress response have been well elucidated. However, the role of TRETs in chitin synthesis remains not fully understood. Here, we identified the HcTRET1 gene from Hyphantria cunea, a major Lepidoptera pest in agriculture and forestry. The role of HcTRET1 in growth and development, especially in chitin synthesis, was discussed by dsRNA-mediated HcTRET1 knockdown. Bioassay showed that HcTRET1 knockdown did not affect larval growth, development and survival in H. cunea, but it significantly reduced the pupa formation rate. Additionally, HcTRET1 silencing increased trehalose levels in the fat body but decreased them in the hemolymph, suggesting HcTRET1 plays a key role in trehalose homeostasis. Moreover, HcTRET1 knockdown significantly downregulated the genes for chitin synthesis (HcGFAT, HcUAP and HcCHSA), resulting in a remarkable reduction of chitin content in the epidermis. Moreover, HcTRET1 knockdown significantly reduced the survival of H. cunea larvae at 42°C. Taken together, these results demonstrated that HcTRET1 played a critical role in larva-pupa transition, in vivo trehalose homeostasis, especially in epidermal chitin biosynthesis in H. cunea larvae. In parallel, its important physiological function in response to high-temperature stress has been verified as well. The findings expand our understanding of the physiological functions of TRET1 in insects, providing a new perspective for trehalose transporters to regulate chitin biosynthesis.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Multi-omics analysis reveals discordant proteome and transcriptome responses in larval guts of Frankliniella occidentalis infected with an orthotospovirus.","authors":"Jinlong Han, Dorith Rotenberg","doi":"10.1111/imb.12992","DOIUrl":"https://doi.org/10.1111/imb.12992","url":null,"abstract":"<p><p>The western flower thrips, Frankliniella occidentalis, is the principal thrips vector of Orthotospovirus tomatomaculae (order Bunyavirales, family Tospoviridae), a devastating plant-pathogenic virus commonly referred to as tomato spotted wilt virus (TSWV). The larval gut is the gateway for virus transmission by F. occidentalis adults to plants. In a previous report, gut expression at the transcriptome level was subtle but significant in response to TSWV in L1s. Since it has been well documented that the relationship between the expression of mRNA and associated protein products in eukaryotic cells is often discordant, we performed identical, replicated experiments to identify and quantify virus-responsive larval gut proteins to expand our understanding of insect host response to TSWV. While we documented statistically significant, positive correlations between the abundance of proteins (4189 identified) and their cognate mRNAs expressed in first and second instar guts, there was virtually no alignment of individual genes identified to be differentially modulated by virus infection at the transcriptome and proteome levels. Predicted protein-protein interaction networks associated with clusters of co-expressed proteins revealed wide variation in correlation strength between protein and cognate transcript abundance, which appeared to be associated with the type of cellular processes, cellular compartments and network connectivity represented by the proteins. In total, our findings indicate distinct and dynamic regulatory mechanisms of transcript and protein abundance (expression, modifications and/or turnover) in virus-infected gut tissues. This study provides molecular candidates for future functional analysis of thrips vector competence and underscores the necessity of examining complex virus-vector interactions at a systems level.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tingwei Pei, Ziwen Gao, Zihao Wang, Han Wang, Chuks F Nwanade, Ziyan Bing, Lu Li, Xiujie Liang, Yuchao Zhang, Yunsheng Tang, Xiaoduan Fang, Zhijun Yu
{"title":"The genome-wide characterisation of cold shock proteins and prominent roles involved in cold response by configuring metabolic pathways in Haemaphysalis longicornis.","authors":"Tingwei Pei, Ziwen Gao, Zihao Wang, Han Wang, Chuks F Nwanade, Ziyan Bing, Lu Li, Xiujie Liang, Yuchao Zhang, Yunsheng Tang, Xiaoduan Fang, Zhijun Yu","doi":"10.1111/imb.12993","DOIUrl":"https://doi.org/10.1111/imb.12993","url":null,"abstract":"<p><p>Cold shock proteins are relatively conserved in evolution and are involved in regulating various life activities, including cell proliferation, nutritional stress and cold adaptation. However, information about the function and regulation of cold shock proteins in ticks during cold response remains meagre. In the present study, six cold shock protein genes were identified from the important vector tick Haemaphysalis longicornis, which were named as HlY-box1, HlY-box2, HlY-box3, HlY-box4, HlY-box5 and HlY-box6. Spatiotemporal expression dynamics revealed dynamic expressions varied significantly after low-temperature treatment, with different expression patterns observed over prolonged exposure periods. Then the function and regulation of cold shock protein genes during the cold response of H. longicornis were explored. RNA interference (RNAi) efficiently knocked down these genes, significantly increasing tick mortality under cold stress. Transcriptomic analysis following HlY-box4 knockdown identified 336 differentially expressed genes (DEGs), which were mainly annotated in the MAPK signalling pathway and metabolism pathway. Proteomic analysis identified 632 differentially expressed proteins associated with ATP-dependent chromatin remodelling, metabolic pathway, spliceosome, ribosome and nucleoplasmic transport pathways. The results highlight the critical roles of cold shock proteins (CSPs) in tick cold responses, primarily through regulating metabolic pathways, and provide a foundation for further exploration of their molecular mechanisms.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mou-Zheng Jiang, Xiao-Xuan Hu, Xu-Dong Li, Qing Wang, Man-Yu Shi, Rui-Rui Cui, Guo-Qing Wei, Lei Wang
{"title":"Knockdown of BmorCPR67 gene disrupts prepupal-pupal transition of silkworm Bombyx mori by thinning the endocuticle.","authors":"Mou-Zheng Jiang, Xiao-Xuan Hu, Xu-Dong Li, Qing Wang, Man-Yu Shi, Rui-Rui Cui, Guo-Qing Wei, Lei Wang","doi":"10.1111/imb.12991","DOIUrl":"https://doi.org/10.1111/imb.12991","url":null,"abstract":"<p><p>The cuticle of insects serves as a crucial organ for preserving body composition, protecting against pathogen invasion, and retaining moisture in their bodies. Cuticular proteins (CPs) are the main constituents of insect cuticles and interact with chitin to form the cuticle's structural framework and mechanical properties. In this study, we investigated the role of a cuticular protein with R&R consensus (CPR), BmorCPR67, a member of the RR-2 subfamily, during the prepupal-to-pupal transition in Bombyx mori. The BmorCPR67 gene exhibited high expression levels during the prepupal stage, with the highest expression detected in the epidermis of the day-1 pupa in B. mori. The expression of the BmorCPR67 gene was induced by 20-hydroxyecdysone (20E). Chitin-binding assays indicated that the BmorCPR67 protein selectively binds to crystalline chitin and chitosan but not to amorphous chitin. Silencing the BmorCPR67 gene disrupted the moulting process from prepupa to pupa, resulting in silkworm mortality. Furthermore, the knockdown of BmorCPR67 altered the expression profiles of key genes involved in chitin metabolism. Notably, significant thinning of the endocuticle was observed 48-96 h after siRNA injection in BmorCPR67-silenced silkworms. These findings highlight the critical role of BmorCPR67 in cuticle development during the prepupal-to-pupal transition in B. mori, contributing to our understanding of the functions of CPs in insect metamorphosis.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hongxiao Yu, Caixia Peng, Zhaohui Chen, Jie Li, Yunqi Li, Xiaojing Zhu, Yuqi Huang, Linlong Jiang, Pablo Sobrado, Jianqiang Lan, Yingying Guo, Qian Han
{"title":"Identification of a serine protease involved in spinosad degradation in the yellow fever mosquito, Aedes aegypti.","authors":"Hongxiao Yu, Caixia Peng, Zhaohui Chen, Jie Li, Yunqi Li, Xiaojing Zhu, Yuqi Huang, Linlong Jiang, Pablo Sobrado, Jianqiang Lan, Yingying Guo, Qian Han","doi":"10.1111/imb.12990","DOIUrl":"https://doi.org/10.1111/imb.12990","url":null,"abstract":"<p><p>Spinosad is a widely used insecticide effective in controlling Aedes aegypti populations, but the molecular mechanisms underlying resistance remain poorly understood. This study explores the role of a serine protease, AeaSP (AAEL002624), in the potential detoxification ability of spinosad. Our results showed the crude protein of Ae. aegypti degraded approximately 48% of spinosad in vitro within 1 h; based on our previous research, AeaSP was believed to be potentially involved in the degradation of spinosad. Subsequently, AeaSP was recombinantly expressed in vitro, and its enzymatic activity was tested using BAEE as a substrate, with a Michaelis constant (KM) of 0.88 mmol/L. Spatiotemporal expression profiles revealed that AeaSP expression peaked in third instar larvae and thoraxes. In vitro assays showed that AeaSP degraded approximately 63% of spinosad (500 ng/mL) within 6 h. RNAi knockdown of AeaSP significantly increased larval mortality under spinosad exposure and raised spinosad residue levels in larvae by 37% under 0.15 μg/mL spinosad. Our findings suggest AeaSP may play a critical role in detoxifying spinosad in Ae. aegypti and serve as a target for improving spinosad efficacy and mosquito control strategies.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jessica Cristina Silva Martins, Héctor Antônio Assunção Romão, Carolina Kurotusch Canettieri, Amanda Caetano Cercilian, Patrícia Rasteiro Ordiale Oliveira, Clelia Ferreira, Walter R Terra, Renata de Oliveira Dias
{"title":"The loss of the urea cycle and ornithine metabolism in different insect orders: An omics approach.","authors":"Jessica Cristina Silva Martins, Héctor Antônio Assunção Romão, Carolina Kurotusch Canettieri, Amanda Caetano Cercilian, Patrícia Rasteiro Ordiale Oliveira, Clelia Ferreira, Walter R Terra, Renata de Oliveira Dias","doi":"10.1111/imb.12989","DOIUrl":"https://doi.org/10.1111/imb.12989","url":null,"abstract":"<p><p>Previous studies suggest that some insects require dietary arginine because they cannot synthesize this amino acid through the urea cycle. To determine whether this finding applies to all insects and what its metabolic implications are, we analysed the conservation of 20 genes involved in arginine biosynthesis and metabolism in the genomes of 150 species from 11 taxonomic orders. Our results showed that no insect can synthesize arginine via the urea cycle, as ornithine carbamoyltransferase is absent from all genomes analysed. While we found losses in other genes encoding urea cycle enzymes, nitric oxide synthase (NOS) was conserved across orders. However, the citrulline produced by NOS cannot be converted back to arginine in several insects due to the loss of argininosuccinate synthase and argininosuccinate lyase genes. Despite the inability to synthesize arginine, all insects (except some Hemiptera) can degrade it to ornithine and urea, as the arginase (ARG) gene is conserved across the orders analysed. For some Hemiptera that have lost ARG, we investigated how these insects produce or metabolize ornithine. Our results show that the genes for converting ornithine to glutamate, proline and putrescine are conserved across orders. However, while all insects have enzymes to synthesize putrescine and spermidine, some lack the ability to produce spermine due to the absence of the spermine synthase gene. Taken together, our results show that the loss of the urea cycle has led to significant changes in the pathways by which insects metabolize and recover arginine, which is particularly important for the diversification of hemipterans.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}