The genome-wide characterisation of cold shock proteins and prominent roles involved in cold response by configuring metabolic pathways in Haemaphysalis longicornis.

IF 2.3 2区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Tingwei Pei, Ziwen Gao, Zihao Wang, Han Wang, Chuks F Nwanade, Ziyan Bing, Lu Li, Xiujie Liang, Yuchao Zhang, Yunsheng Tang, Xiaoduan Fang, Zhijun Yu
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引用次数: 0

Abstract

Cold shock proteins are relatively conserved in evolution and are involved in regulating various life activities, including cell proliferation, nutritional stress and cold adaptation. However, information about the function and regulation of cold shock proteins in ticks during cold response remains meagre. In the present study, six cold shock protein genes were identified from the important vector tick Haemaphysalis longicornis, which were named as HlY-box1, HlY-box2, HlY-box3, HlY-box4, HlY-box5 and HlY-box6. Spatiotemporal expression dynamics revealed dynamic expressions varied significantly after low-temperature treatment, with different expression patterns observed over prolonged exposure periods. Then the function and regulation of cold shock protein genes during the cold response of H. longicornis were explored. RNA interference (RNAi) efficiently knocked down these genes, significantly increasing tick mortality under cold stress. Transcriptomic analysis following HlY-box4 knockdown identified 336 differentially expressed genes (DEGs), which were mainly annotated in the MAPK signalling pathway and metabolism pathway. Proteomic analysis identified 632 differentially expressed proteins associated with ATP-dependent chromatin remodelling, metabolic pathway, spliceosome, ribosome and nucleoplasmic transport pathways. The results highlight the critical roles of cold shock proteins (CSPs) in tick cold responses, primarily through regulating metabolic pathways, and provide a foundation for further exploration of their molecular mechanisms.

冷休克蛋白的全基因组特征和通过配置长角血蜱的代谢途径参与冷反应的突出作用。
冷休克蛋白在进化过程中相对保守,参与调节多种生命活动,包括细胞增殖、营养应激和冷适应。然而,关于蜱在寒冷反应中冷休克蛋白的功能和调控的信息仍然很少。本研究从长角血蜱重要载体中分离到6个冷休克蛋白基因,分别命名为HlY-box1、HlY-box2、HlY-box3、HlY-box4、HlY-box5和HlY-box6。时空表达动态分析表明,低温处理后动态表达变化显著,随着低温处理时间的延长,动态表达模式也有所不同。探讨了冷休克蛋白基因在长角天牛冷反应中的功能及调控。RNA干扰(RNAi)有效地敲除了这些基因,显著增加了蜱在寒冷胁迫下的死亡率。HlY-box4敲低后的转录组学分析鉴定出336个差异表达基因(DEGs),这些差异表达基因主要在MAPK信号通路和代谢途径中有注释。蛋白质组学分析鉴定出632个与atp依赖性染色质重塑、代谢途径、剪接体、核糖体和核质转运途径相关的差异表达蛋白。这些结果突出了冷休克蛋白(CSPs)在蜱寒冷反应中的关键作用,主要通过调节代谢途径,并为进一步探索其分子机制提供了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Insect Molecular Biology
Insect Molecular Biology 生物-昆虫学
CiteScore
4.80
自引率
3.80%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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