Insect Molecular Biology最新文献

The homeobox gene Distal-less is critical for the development of adult body appendages in Bombyx mori. 同源盒基因远端缺失是家蚕成体附属物发育的关键基因。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-07-14 DOI: 10.1111/imb.13015

Guan Man, Peilin Guo, Yutong Liu, Hongxia Kang, Kai Chen, Ye Yu, Anjiang Tan

{"title":"The homeobox gene Distal-less is critical for the development of adult body appendages in Bombyx mori.","authors":"Guan Man, Peilin Guo, Yutong Liu, Hongxia Kang, Kai Chen, Ye Yu, Anjiang Tan","doi":"10.1111/imb.13015","DOIUrl":"https://doi.org/10.1111/imb.13015","url":null,"abstract":"The development of adult body appendages is critical for insects. The homeobox gene Distal-less (Dll) is known for its role in proximodistal patterning, and its role in the development of the appendage has been well studied in the model organism Drosophila melanogaster. However, the biological function of Dll in other insects still remains to be further verified. In this study, we investigated the biological function of Dll in the lepidoptera model insect Bombyx mori. Similar to the expression patterns observed in D. melanogaster, BmDll was highly expressed in the tibia and tarsus of the legs. The BmDll mutants obtained through the binary transgenic CRISPR/Cas9 system appeared to thrive normally and no apparent deleterious phenotypes were observed throughout the embryonic and larval stages. Nevertheless, mutants exhibited severe defects in the development of the appendage, especially at the distal end of legs, from pupal stage to adult stage. RNA-seq analysis revealed that metabolism pathways, extracellular matrix (ECM)-receptor interaction and structural constituent of the cuticle were dysregulated in mutant silkworms. Our study provided genetic evidence that Dll plays an important role in the development of B. mori adult body appendages.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transformer-2 gene is essential for female and male development in Gryllus bimaculatus. 转换-2基因在双斑斑马鱼雌雄发育中起着重要作用。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-07-10 DOI: 10.1111/imb.13016

Li-Dong Shi, Jia-Xin Duan, Ning Zhang, Gang-Qi Fang, Zhu-Qing He, Kai Li

引用次数: 0

RNAi comes of age: Translating insect molecular insights into field applications RNAi时代来临：将昆虫分子见解转化为实地应用

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-07-08 DOI: 10.1111/imb.13012

Umut Toprak

引用次数: 0

CRISPR/Cas9-mediated germline mutagenesis in the subsocial parasitoid wasp, Sclerodermus guani. CRISPR/ cas9介导的瓜硬皮亚社会性寄生蜂种系突变

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-06-28 DOI: 10.1111/imb.13014

Zi Ye, Guanzhen Fan, Yun Wei, Li Li, Feng Liu

{"title":"CRISPR/Cas9-mediated germline mutagenesis in the subsocial parasitoid wasp, Sclerodermus guani.","authors":"Zi Ye, Guanzhen Fan, Yun Wei, Li Li, Feng Liu","doi":"10.1111/imb.13014","DOIUrl":"https://doi.org/10.1111/imb.13014","url":null,"abstract":"The ectoparasitoid wasp Sclerodermus guani (Hymenoptera: Bethylidae), as a subsocial insect, is widely applied in biological control against beetle vectors of pine wood nematodes. Despite significant advances in behavioural research, functional genetics in S. guani remains underdeveloped due to the absence of efficient gene manipulation tools. In this study, we employed CRISPR-mediated mutagenesis to achieve germline gene knockout targeting the eye pigment-associated gene kynurenine 3-monooxygenase (KMO). Phylogenetic analysis revealed that S. guani KMO shares a close relationship with its homologue in Prorops nasuta (Hymenoptera: Bethylidae). Two single-guide RNAs (sgRNAs), coupled with Cas9 protein with and without nuclear localisation signal (NLS) were tested. Both sgRNAs induced specific in vitro DNA cleavage and in vivo heritable indels at the target genomic loci. Homozygous null mutant females and males exhibit a white-eye phenotype, which was identified during pupal stage. Optimal editing efficiency in vivo was achieved using the Cas9-NLS variant. Given the complication of germline gene editing in eusocial Hymenopterans, the application of CRISPR in the subsocial parasitoid wasp S. guani provides an accessible research platform for the molecular evolution of insect sociality.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TcCYP4C1 in combination with trehalose to cope with high-CO₂ stress in Tribolium castaneum (Coleoptera). TcCYP4C1与海藻糖联合作用对褐藻高co2胁迫的影响。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-06-28 DOI: 10.1111/imb.12997

Xinyu Zhang, Liwen Guan, Yuya Zhang, Fan Zhong, Yuhang Xie, Yi Zhang, Xinyi Zhang, Min Zhou, Can Li, Bin Tang

{"title":"TcCYP4C1 in combination with trehalose to cope with high-CO2 stress in Tribolium castaneum (Coleoptera).","authors":"Xinyu Zhang, Liwen Guan, Yuya Zhang, Fan Zhong, Yuhang Xie, Yi Zhang, Xinyi Zhang, Min Zhou, Can Li, Bin Tang","doi":"10.1111/imb.12997","DOIUrl":"https://doi.org/10.1111/imb.12997","url":null,"abstract":"Cytochrome P450 monooxygenase (CYP), an important detoxifying enzyme in insects, is involved in the metabolism and degradation of both exogenous compounds and endogenous substances. To investigate the involvement of the T. castaneum TcCYP4C1 gene in detoxification metabolism under high CO2 and the protective role of trehalose against hypoxic stress in insects. In the present study, after successfully silencing the TcCYP4C1 gene of T. castaneum by double-stranded RNA(dsRNA), the larvae were exposed to 95% CO2. This exposure resulted in a statistically significant increase in larval mortality and a significant elevation in the activity of the carboxylesterase enzyme (CarE). However, a decrease in mortality from 18.15% to 11.24% was observed when larvae were fed trehalose after dsRNA injection. In addition, the gene expression levels of the trehalose metabolism pathway related genes TRE1-3, TRE1-4 and TPS2 were significantly up-regulated after 95% CO2 treatment. In summary, the TcCYP4C1 gene emerges as a pivotal factor in the adaptive response of T. castaneum to high CO2. Trehalose effectively mitigates the detrimental effects resulting from the silencing of TcCYP4C1 and exposure to high CO2 stress in T. castaneum. Our findings not only establish a theoretical foundation for the development of novel pesticides tailored for low-oxygen grain storage environments but also inspire innovative, environmentally sustainable pest management strategies in the grain storage sector.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcriptome of the ant Temnothorax nylanderi is not affected by urbanisation but by rearing conditions. 蚂蚁Temnothorax nylanderi的转录组不受城市化的影响，但受饲养条件的影响。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-06-28 DOI: 10.1111/imb.13011

Nicholas M A Smith, Lauren Jacquier, Elise Gay, Mathieu Molet, Claudie Doums

{"title":"The transcriptome of the ant Temnothorax nylanderi is not affected by urbanisation but by rearing conditions.","authors":"Nicholas M A Smith, Lauren Jacquier, Elise Gay, Mathieu Molet, Claudie Doums","doi":"10.1111/imb.13011","DOIUrl":"https://doi.org/10.1111/imb.13011","url":null,"abstract":"Urbanisation alters the environment of organisms, creating new challenges and opportunities. In ants, urbanisation has been shown to increase resistance to temperature and to pollutants. Here we assessed how urbanisation affects gene expression in workers and queens of the ant Temnothorax nylanderi, a species that inhabits both city and forest habitats. We further examined whether there was a differential expression between two distinct conditions, in the field or after laboratory rearing for 8 months. As expected, the number of differentially expressed genes (DEGs) between workers and queens was large (2830 genes). However, we found that habitat (city or forest) had no clear influence on gene expression in queens and workers of T. nylanderi even when individuals were directly collected from the field. Interestingly, we also found 661 DEGs between field colonies and laboratory-reared ones. The effect of rearing conditions on gene activity was greater in workers than in queens, suggesting that queens are more resilient to environmental changes. The lack of transcriptional differences between habitats is intriguing because of the previously observed differences in life-history traits between city and forest populations.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new piece in the repeatome puzzle of Triatominae bugs: The analysis of Triatoma rubrofasciata reveals the role of satellite DNAs in the karyotypic evolution of distinct lineages. 三角蝽科昆虫重复染色体谜题的新发现：对红膜三角蝽的分析揭示了卫星dna在不同谱系的核型进化中的作用。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-06-27 DOI: 10.1111/imb.13013

Sebastián Pita, Pablo Mora, José M Rico-Porras, Diogo C Cabral-de-Mello, Francisco J Ruiz-Ruano, Teresa Palomeque, Ho Viet Hieu, Francisco Panzera, Pedro Lorite

{"title":"A new piece in the repeatome puzzle of Triatominae bugs: The analysis of Triatoma rubrofasciata reveals the role of satellite DNAs in the karyotypic evolution of distinct lineages.","authors":"Sebastián Pita, Pablo Mora, José M Rico-Porras, Diogo C Cabral-de-Mello, Francisco J Ruiz-Ruano, Teresa Palomeque, Ho Viet Hieu, Francisco Panzera, Pedro Lorite","doi":"10.1111/imb.13013","DOIUrl":"https://doi.org/10.1111/imb.13013","url":null,"abstract":"The genome of Triatoma rubrofasciata, a representative of the North American Triatomini lineage, was analysed to characterise its repetitive DNA content and satellite DNA (satDNA) organisation. Using RepeatExplorer2, we determined that repetitive elements comprise approximately 25% of the genome in a male sample from Vietnam and 16% in a female sample from China, with satDNA being the most abundant component. The satellitome analysis revealed 126 satDNA families in the male and 114 in the female, with marked quantitative differences driven by the amplification of two satDNA families: TrubSat001-166 and TrubSat002-9. Fluorescence in situ hybridization (FISH) confirmed that TrubSat002-9 is enriched in the Y chromosome, explaining its lesser abundance in the female genome. Chromosomal mapping revealed three distribution patterns of satDNA: (i) localisation in autosomal heterochromatin, (ii) restriction to the Y chromosome, and (iii) presence in euchromatin. SatDNA landscapes showed sharp peaks at low divergence values, consistent with recent amplifications in heterochromatic regions, and broader peaks at higher divergence levels, suggesting older satDNAs located in euchromatic regions. Additionally, several satDNA families are conserved among T. rubrofasciata, T. infestans, T. delpontei and Rhodnius prolixus, supporting the \"library hypothesis\" of satDNA evolution. Our findings highlight the differential amplification of satDNA families linked to heterochromatin expansion, particularly in autosomes, and the conservation of Y-linked repeats. This study provides new insights into the dynamic role of satDNAs in the karyotypic evolution of Triatominae bugs.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of copper exposure on the silkworm Bombyx mori: Insights into the changes of metabolism, gut microbial composition and gene expression. 铜暴露对家蚕代谢、肠道微生物组成和基因表达的影响。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-06-27 DOI: 10.1111/imb.13010

Dan-Dan Bian, Yan-Xia Shi, Yang Ye, Geng-Yu Zhang, Xiao-Li Sun, Qiu-Ning Liu, Bo-Ping Tang, Bao-Jian Zhu

{"title":"Effect of copper exposure on the silkworm Bombyx mori: Insights into the changes of metabolism, gut microbial composition and gene expression.","authors":"Dan-Dan Bian, Yan-Xia Shi, Yang Ye, Geng-Yu Zhang, Xiao-Li Sun, Qiu-Ning Liu, Bo-Ping Tang, Bao-Jian Zhu","doi":"10.1111/imb.13010","DOIUrl":"https://doi.org/10.1111/imb.13010","url":null,"abstract":"Copper (Cu), a trace element with crucial roles in physiological processes, can exert detrimental effects when imbalanced. Despite growing research on Cu's impacts on biota, its effects on terrestrial organisms, particularly insects, at environmental concentrations remain poorly understood. This study aims to elucidate the mechanisms underlying Cu-induced damage in silkworms (Bombyx mori L., 1758 (Lepidoptera: Bombycidae)) and identify potential targets to mitigate such damage. Using an integrated approach of physiological, histopathological, biochemical and multi-omics analyses, we investigated the effects of Cu exposure throughout most of the silkworms' larval stage. Our findings reveal that Cu exposure significantly hampers the growth and development of silkworms, evidenced by reduced intestinal trehalose levels, compromised peritrophic membrane (PM) structure in the midgut (MG) and altered composition and diversity of the intestinal microbiota. Furthermore, Cu exposure leads to an increase in pathogenic bacteria and a decrease in probiotics and induces inflammation and apoptosis in the midgut and fatbody (FB) tissues. Biochemical and transcriptomic analyses indicate that Cu disrupts nutrient metabolism and energy homeostasis, resulting in decreased adenosine triphosphatase (ATP) levels. Chronic Cu exposure activates the PI3K/AKT/mTOR signalling pathway, triggering abnormal apoptosis and autophagy, altering detoxification processes, immune enzyme activities and gene expression. This study provides novel insights into the mechanisms of Cu toxicity in silkworms and establishes a foundation for identifying targets to reduce Cu's biotoxicity, offering valuable data for managing Cu pollution in insects.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-genome resequencing reveals genetic evolution of honeybees (Apis cerana) in high-altitude and overwintering adaptation. 全基因组重测序揭示了蜜蜂（Apis cerana）在高海拔和越冬适应方面的遗传进化。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-06-20 DOI: 10.1111/imb.13009

Fugui Li, Xujiang He, Lizhen Zhang, Junfeng Liu, Zilong Wang, Zhijiang Zeng

{"title":"Whole-genome resequencing reveals genetic evolution of honeybees (Apis cerana) in high-altitude and overwintering adaptation.","authors":"Fugui Li, Xujiang He, Lizhen Zhang, Junfeng Liu, Zilong Wang, Zhijiang Zeng","doi":"10.1111/imb.13009","DOIUrl":"10.1111/imb.13009","url":null,"abstract":"Over time, populations of Apis cerana across diverse geographical regions have undergone distinct phenotypic adaptations in response to varied climates, arising from prolonged natural evolutionary processes. Understanding the molecular genetic underpinnings and the implications of climate change on the adaptive evolution of A. cerana is crucial for its conservation amidst environmental shifts. Here, we conducted a comprehensive analysis of 110 bee individuals sourced from 11 regions at comparable temperatures, environments, and altitudes. A total of 525.56 Gb of filter-trimmed sequencing data were utilised for population genetic analysis of honeybees. The findings indicate genetic differentiation among bee populations from various geographical regions, with higher levels of population divergence observed in different altitudinal environments. Particularly noteworthy is the significant genetic divergence of the bee population from Haikou (HK) compared to populations in other areas, characterised by higher inbreeding coefficients and runs of homozygosity (ROHs), as well as lower nucleotide diversity. However, populations from Guangzhou (GZ), Jinzhai (JZ), Qimen (QM), Shennongjia (SNJ), and Suzhou (SZ) exhibit a close genetic relationship. Using comparisons across multiple bee populations from different groups, we identified selective signatures across different environmental temperatures and altitudes. Furthermore, by integrating genomic selection signals with comparative transcriptomic analysis, we identified genes potentially involved in bee adaptation to high altitude, such as g9950.t1 (alcohol dehydrogenase), g5267.t1 (diacylglycerol kinase theta-like isoform 2), gene g4025.t1 (Tyrosine 3-monooxygenase), and g3609.t1 (heme oxygenase). Our results indicate that both temperature and altitude contribute to the genetic differentiation and geographical adaptability of A. cerana populations.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and functional characterisation of a novel antimicrobial peptide from the housefly, Musca domestica. 家蝇一种新型抗菌肽的鉴定和功能表征。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-06-19 DOI: 10.1111/imb.13005

Dongdong Lin, Shuangshuang Li, Shiying Li, Ting Tang, Fengsong Liu

{"title":"Identification and functional characterisation of a novel antimicrobial peptide from the housefly, Musca domestica.","authors":"Dongdong Lin, Shuangshuang Li, Shiying Li, Ting Tang, Fengsong Liu","doi":"10.1111/imb.13005","DOIUrl":"https://doi.org/10.1111/imb.13005","url":null,"abstract":"Antimicrobial peptides (AMPs) are increasingly being recognised as promising alternatives to conventional antibiotics due to their distinctive antimicrobial mechanisms and reduced likelihood of inducing drug resistance. Insects represent a significant source of AMPs. In this study, a potential AMP gene, MdAMP5, was identified based on its strong immunoinducibility and the presence of a signal peptide, and an amphipathic α-helix in the encoded protein. MdAMP5 encoded a 50-amino acid precursor protein with an N-terminal 22-amino acid signal peptide. The calculated molecular mass of the mature protein was 2.92 kDa, with an estimated isoelectric point of 6.23. Structural analyses revealed that the N-terminus of mature MdAMP5 contained an irregularly coiled segment, while the C-terminus featured an amphipathic α-helix with a glycine-lysine residue at the end. Furthermore, the MdAMP5 gene was successfully expressed in the yeast Pichia pastoris, and the recombinant MdAMP5 (rMdAMP5) protein exhibited effective and broad-spectrum antimicrobial activity against both Gram-positive and Gram-negative bacteria in vitro and in vivo. Treatment with rMdAMP5 resulted in significant changes in bacterial morphology, including cell lysis and deformation of bacteriophages. In conclusion, this study identified and successfully expressed a novel AMP that showed low cytotoxicity to mammalian cells and high selectivity towards bacterial cells. This research offers a new candidate for therapeutic drug development, and enhances the understanding of the mechanism and application of AMPs.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0