Insect Molecular Biology最新文献

Bombyx mori nucleopolyhedrovirus LEF-2 disrupts the cell cycle in the G2/M phase by triggering a host cell DNA damage response. 蚕核多面体逆转录病毒 LEF-2 通过触发宿主细胞 DNA 损伤反应，在 G2/M 阶段破坏细胞周期。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-02-01 Epub Date: 2024-08-16 DOI: 10.1111/imb.12951

Jie Wang, Miao Xiao, Zhigang Hu, Yu Lin, Kejie Li, Peng Chen, Cheng Lu, Zhanqi Dong, Minhui Pan

{"title":"Bombyx mori nucleopolyhedrovirus LEF-2 disrupts the cell cycle in the G2/M phase by triggering a host cell DNA damage response.","authors":"Jie Wang, Miao Xiao, Zhigang Hu, Yu Lin, Kejie Li, Peng Chen, Cheng Lu, Zhanqi Dong, Minhui Pan","doi":"10.1111/imb.12951","DOIUrl":"10.1111/imb.12951","url":null,"abstract":"It is a common strategy for viruses to block the host cell cycle to favour their DNA replication. Baculovirus, being a double-stranded DNA virus, can arrest the cell cycle in the G2/M phase to facilitate its replication. However, the key viral genes and mechanisms crucial for inducing cell cycle arrest remain poorly understood. Here, we initially examined the impacts of several Bombyx mori nucleopolyhedrovirus (BmNPV) DNA replication-associated genes: ie1, lef-1, lef-2, lef-3, lef-4, odv-ec27 and dbp. We assessed their effects on both the host cells' DNA replication and cell cycle. Our findings reveal that when the lef-2 gene was overexpressed, it led to a significant increase in the number of cells in the G2/M phase and a reduction in the number of cells in the S phase. Furthermore, we discovered that the LEF-2 protein is located in the virogenic stroma and confirmed its involvement in viral DNA replication. Additionally, by employing interference and overexpression experiments, we found that LEF-2 influences host cell DNA replication and blocks the cell cycle in the G2/M phase by regulating the expression of CyclinB and CDK1. Finally, we found that BmNPV lef-2 triggered a DNA damage response in the host cell, and inhibiting this response removed the cell cycle block caused by BmNPV LEF-2. Thus, our findings indicate that the BmNPV lef-2 gene plays a crucial role in viral DNA replication and can regulate host cell cycle processes. This study furthers our understanding of baculovirus-host cell interactions and provides new insight into the molecular mechanisms of antiviral research.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":"81-93"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141987893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas9-mediated knockout of the abdominal-B homeotic gene in the global pest, fall armyworm (Spodoptera frugiperda). CRISPR/Cas9 介导的全球害虫--秋军虫（Spodoptera frugiperda）腹部-B 同源基因的敲除。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-02-01 Epub Date: 2024-09-24 DOI: 10.1111/imb.12958

Xiao-Guang Liu, Te Zhao, Qi-Qi Qiu, Cong-Ke Wang, Tian-Liang Li, Xiao-Long Liu, Li Wang, Qin-Qin Wang, Lin Zhou

{"title":"CRISPR/Cas9-mediated knockout of the abdominal-B homeotic gene in the global pest, fall armyworm (Spodoptera frugiperda).","authors":"Xiao-Guang Liu, Te Zhao, Qi-Qi Qiu, Cong-Ke Wang, Tian-Liang Li, Xiao-Long Liu, Li Wang, Qin-Qin Wang, Lin Zhou","doi":"10.1111/imb.12958","DOIUrl":"10.1111/imb.12958","url":null,"abstract":"The Homeotic complex (Hox) genes play a crucial role in determining segment identity and appendage morphology in bilaterian animals along the antero-posterior axis. Recent studies have expanded to agricultural pests such as fall armyworm (FAW), scientifically known as Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae), which significantly threatens global agricultural productivity. However, the specific role of the hox gene Sfabd-B in FAW remains unexplored. This research investigates the spatial and temporal expression patterns of Sfabd-B in various tissues at different developmental stages using quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, we explored the potential function of the Sfabd-B gene located in the FAW genome using CRISPR/Cas9 technology. The larval mutant phenotypes can be classified into three subgroups as compared with wild-type individuals, that is, an excess of pedis in the posterior abdomen, deficient pedis due to segmental fusion and deviations in the posterior abdominal segments. Importantly, significant differences in mutant phenotypes between male and female individuals were also evident during the pupal and adult phases. Notably, both the decapentaplegic (dpp) and cuticular protein 12 (cp 12) genes displayed a substantial marked decrease in expression levels in the copulatory organ of male mutants and the ovipositor of female mutants compared with the wild type. These findings highlight the importance of Sfabd-B in genital tract patterning, providing a potential target for improving genetic control.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":"162-173"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sex-specific homologue of waprin is essential for embryonic development in the red flour beetle, Tribolium castaneum. 在红面粉甲虫（Tribolium castaneum）的胚胎发育过程中，waprin的性别特异性同源物是必不可少的。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-02-01 Epub Date: 2024-08-21 DOI: 10.1111/imb.12956

Chhavi Choudhary, Divyanshu Kishore, Keshav Kumar Meghwanshi, Vivek Verma, Jayendra Nath Shukla

{"title":"A sex-specific homologue of waprin is essential for embryonic development in the red flour beetle, Tribolium castaneum.","authors":"Chhavi Choudhary, Divyanshu Kishore, Keshav Kumar Meghwanshi, Vivek Verma, Jayendra Nath Shukla","doi":"10.1111/imb.12956","DOIUrl":"10.1111/imb.12956","url":null,"abstract":"Waprin, a WAP (Whey acidic protein) domain-containing extracellular secretory protein, is widely known for its antibacterial properties. In this study, a waprin homologue (Tc_wapF) expressing in a female-specific manner was identified in Tribolium castaneum, through the analysis of sex-specific transcriptomes. Developmental- and tissue-specific profiling revealed the widespread expression of Tc_wapF in adult female tissues, particularly in the ovary, gut and fatbody. This female-specific expression of Tc_wapF is not regulated by the classical sex-determination cascade of T. castaneum, as we fail to get any attenuation in Tc_wapF transcript levels in Tcdsx and Tctra (key players of sex determination cascade of T. castaneum) knockdown females. RNA interference-mediated knockdown of Tc_wapF in females led to the non-hatching of eggs laid by these females, suggesting the crucial role of Tc_wapF in the embryonic development in T. castaneum. This is the first report on the identification of a sex-specific waprin homologue in an insect and its involvement in embryonic development. Future investigations on the functional conservation of insect waprins and their mechanistic role in embryonic development can be exploited for improving pest management strategies.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":"111-121"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142017313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Rpfor gene modulates the locomotory activity and host-seeking behaviour of Rhodnius prolixus. Rpfor基因调节了Rhodnius prolixus的运动活性和寻找宿主的行为。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-02-01 Epub Date: 2024-08-08 DOI: 10.1111/imb.12952

Newmar P Marliére, Marcelo G Lorenzo, Alessandra A Guarneri

{"title":"The Rpfor gene modulates the locomotory activity and host-seeking behaviour of Rhodnius prolixus.","authors":"Newmar P Marliére, Marcelo G Lorenzo, Alessandra A Guarneri","doi":"10.1111/imb.12952","DOIUrl":"10.1111/imb.12952","url":null,"abstract":"The molecular bases of animal behaviour are intricate due to the pleiotropic nature of behaviour-modulating genes, which are often expressed across multiple tissues. The foraging gene (for) encodes a cGMP-dependent protein kinase (PKG), pivotal in regulating downstream target proteins through phosphorylation. In insects, for has been implicated in various behavioural contexts and physiological processes regarding searching for food. Rhodnius prolixus, a hematophagous bug that transmits Trypanosoma cruzi, the causative agent of Chagas disease, exhibits specific activity patterns associated with its hematophagous behaviour. Our previous work demonstrated a correlation between locomotor activity profiles and the expression of Rpfor, suggesting its involvement in modulating triatomine locomotion. In this study, we investigated the impact of Rpfor knockdown on locomotory activity, host-seeking behaviour, feeding performance and lipid metabolism in R. prolixus nymphs. Using RNA interference, we achieved a significant reduction of Rpfor expression in both the brain and fat body of R. prolixus nymphs. Knocked-down nymphs exhibited diminished non-oriented locomotory activity compared with controls, without altering the characteristic bimodal pattern of activity. Additionally, they displayed an increased tendency to approach a host, suggesting a role for Rpfor in modulating host-seeking behaviour. Feeding performance and lipid metabolism remained unaffected by Rpfor knockdown. Our findings underscore the multifaceted role of Rpfor in modulating locomotor activity and host-seeking behaviour in R. prolixus nymphs, shedding light on the molecular mechanisms underlying their hematophagous behaviour and potential implications for disease transmission. Further research is necessary to elucidate the intricate interplay between Rpfor expression, behaviour and physiological processes in triatomine bugs.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":"94-103"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding the transgene expression toolbox of the malaria vector Anopheles stephensi. 扩展疟疾病媒雅典按蚊的转基因表达工具箱。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-02-01 Epub Date: 2024-08-11 DOI: 10.1111/imb.12953

Joshua Southworth, Estela Gonzalez, Katherine Nevard, Mireia Larrosa-Godall, Luke Alphey, Michelle A E Anderson

{"title":"Expanding the transgene expression toolbox of the malaria vector Anopheles stephensi.","authors":"Joshua Southworth, Estela Gonzalez, Katherine Nevard, Mireia Larrosa-Godall, Luke Alphey, Michelle A E Anderson","doi":"10.1111/imb.12953","DOIUrl":"10.1111/imb.12953","url":null,"abstract":"Anopheles stephensi Liston, 1901 (Diptera: culicidae) is a competent vector of Plasmodium falciparum (Haemosporida: plasmodiidae) malaria, and its expansion in the African continent is of concern due to its viability in urban settings and resistance to insecticides. To enhance its genetic tractability, we determined the utility of a ~2 kb An. stephensi lipophorin (lp) promoter fragment in driving transgene expression. Lipophorin genes are involved in lipid transport in insects, and an orthologous promoter in An. gambiae (AGAP001826) was previously demonstrated to successfully express a transgene. In the present study, we qualitatively characterised the expression of a ZsYellow fluorescent marker protein, expressed by An. stephensi lp promoter fragment. Our study indicated that the lp promoter fragment was effective, generating a distinct expression pattern in comparison to the commonly utilised 3xP3 promoter. The lp:ZsYellow fluorescence was largely visible in early instar larvae and appeared more intense in later instar larvae, pupae and adults, becoming especially conspicuous in adult females after a blood meal. Different isolines showed some variation in expression pattern and intensity. Aside from general transgene expression, as the lp promoter produces a suitable fluorescent protein marker expression pattern, it may facilitate genotypic screening and aid the development of more complex genetic biocontrol systems, such as multi-component gene drives. This study represents an expansion of the An. stephensi genetic toolbox, an important endeavour to increase the speed of An. stephensi research and reach public health milestones in combating malaria.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":"104-110"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705503/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in bumblebee queen gut microbiotas during and after overwintering diapause. 越冬停歇期间和之后熊蜂蜂后肠道微生物群的变化

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-02-01 Epub Date: 2024-08-22 DOI: 10.1111/imb.12957

Michelle Z Hotchkiss, Jessica R K Forrest, Alexandre J Poulain

{"title":"Changes in bumblebee queen gut microbiotas during and after overwintering diapause.","authors":"Michelle Z Hotchkiss, Jessica R K Forrest, Alexandre J Poulain","doi":"10.1111/imb.12957","DOIUrl":"10.1111/imb.12957","url":null,"abstract":"Bumblebees are key pollinators with gut microbiotas that support host health. After bumblebee queens undergo winter diapause, which occurs before spring colony establishment, their gut microbiotas are disturbed, but little is known about community dynamics during diapause itself. Queen gut microbiotas also help seed worker microbiotas, so it is important that they recover post-diapause to a typical community structure, a process that may be impeded by pesticide exposure. We examined how bumblebee queen gut microbiota community structure and metabolic potential shift during and after winter diapause, and whether post-diapause recovery is affected by pesticide exposure. To do so, we placed commercial Bombus impatiens queens into diapause, euthanizing them at 0, 2 and 4 months of diapause. Additionally, we allowed some queens to recover from diapause for 1 week before euthanasia, exposing half to the common herbicide glyphosate. Using whole-community, shotgun metagenomic sequencing, we found that core bee gut phylotypes dominated queen gut microbiotas before, during and after diapause, but that two phylotypes, Schmidhempelia and Snodgrassella, ceased to be detected during late diapause and recovery. Despite fluctuations in taxonomic community structure, metabolic potential remained constant through diapause and recovery. Also, glyphosate exposure did not affect post-diapause microbiota recovery. However, metagenomic assembly quality and our ability to detect microbial taxa and metabolic pathways declined alongside microbial abundance, which was substantially reduced during diapause. Our study offers new insights into how bumblebee queen gut microbiotas change taxonomically and functionally during a key life stage and provides guidance for future microbiota studies in diapausing bumblebees.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":"136-150"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142035757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterisation of the bacteriomes harboured by major wireworm pest species in the Canadian Prairies. 加拿大草原主要线虫害虫物种所含细菌群的特征。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-02-01 Epub Date: 2024-10-09 DOI: 10.1111/imb.12962

Ivan Drahun, Keagan Morrison, Elise A Poole, Willem G van Herk, Bryan J Cassone

{"title":"Characterisation of the bacteriomes harboured by major wireworm pest species in the Canadian Prairies.","authors":"Ivan Drahun, Keagan Morrison, Elise A Poole, Willem G van Herk, Bryan J Cassone","doi":"10.1111/imb.12962","DOIUrl":"10.1111/imb.12962","url":null,"abstract":"Nearly all insects harbour bacterial communities that can have a profound effect on their life history, including regulating and shaping host metabolism, development, immunity and fitness. The bacteriomes of several coleopterans have been described; however, very little has been reported for wireworms. These long-lived larvae of click beetles (Coleoptera: Elateridae) are major agricultural pests of a variety of crops grown in the Canadian Prairies. Consequently, the goal of this study was to characterise the bacteriomes of five of the most significant pest species within the region: Limonius californicus, Hypnoidus abbreviatus, H. bicolor, Aeolus mellillus and Dalopius spp. To do this, we collected larvae from southern Manitoba fields (pre-seeding) and carried out 16S rRNA sequencing on individual specimens. Our results indicate wireworms have diverse and taxon-rich bacterial communities, with over 400 genera identified predominately from the phyla Proteobacteria, Actinobacteriota, Bacteroidota and Firmicutes. However, each species had nine or fewer genera comprising >80% of their bacteriome. Network analyses revealed some community structuring consistent among species, which may culminate in shaping/regulating host biology. Moreover, the microbial signatures were influenced by both ontogeny (early vs. late stage larvae) and reproductive strategy (sexual vs. parthenogenetic), with a myriad of other factors likely contributing to bacterial diversity that are impossible to resolve from our study. Overall, this metagenomics study represents the first to characterise the bacteriomes of wireworms in the Canadian Prairies and the findings could assist in the development of sustainable management strategies for these important agricultural pests.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":"203-217"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Foraging in the darkness: Highly selective tuning of below-ground larval olfaction to Brassicaceae volatiles in striped flea beetle. 在黑暗中觅食：条斑跳甲地下幼虫嗅觉对十字花科挥发性物质的高度选择性调节

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-02-01 Epub Date: 2024-09-22 DOI: 10.1111/imb.12960

Yong Xiao, Chunmei Lei, Xue Wang, Raufa Batool, Fei Yin, Zhengke Peng, Xiangfeng Jing, Zhenyu Li

{"title":"Foraging in the darkness: Highly selective tuning of below-ground larval olfaction to Brassicaceae volatiles in striped flea beetle.","authors":"Yong Xiao, Chunmei Lei, Xue Wang, Raufa Batool, Fei Yin, Zhengke Peng, Xiangfeng Jing, Zhenyu Li","doi":"10.1111/imb.12960","DOIUrl":"10.1111/imb.12960","url":null,"abstract":"The olfactory system of above-ground insects is among the best described perceptual architectures. However, remarkably little is known about how below-ground insects navigate in the dark for foraging. Here, we investigated host plant preferences, olfactory sensilla and characterise olfactory proteins in below-ground larvae of the striped flea beetle (SFB) Phyllotreta striolata Fabricius (Coleoptera: Chrysomelidae). Both the adults and larvae of this coleopteran pest cause serious damage to Brassicaceous crops above and below ground, respectively. To elucidate the role of olfactory system in host location of below-ground larvae, we initially demonstrated that SFB larvae distinctly favoured Brassicaceae over other plant families by two-choice behavioural bioassay. Subsequently, scanning electron microscopy of sensilla in SFB larval head showed a significant reduction in the number of olfactory sensilla in larvae compared with adults. However, essential olfactory sensilla such as sensilla basiconica are underscoring the indispensability of the larval olfactory system. We selected four larval-specific odorant binding proteins for functional validation from our previous transcriptome data. Functional studies revealed that PstrOBP23 exhibits robust binding affinity to 24 volatiles of Brassicaceae plants, including seven isothiocyanate compounds. This suggests a pivotal role of PstrOBP23 in the foraging behaviour of the larvae below the ground. Moreover, two ligands displaying strong binding capacity exhibit apparent attractive or repellent activity towards SFB larvae. Our findings provide a crucial insight into the olfactory system of below-ground larvae in SFB, highlighting the highly selective tuning of larvae specific OBP to host plant volatiles. These results offer potential avenues for developing effective pest control strategies against SFB.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":"151-161"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142286194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of a novel thioredoxin-related transmembrane protein gene AcTMX3 that plays important roles in antioxidant defence in Arma chinensis diapause. 新型硫氧还蛋白相关跨膜蛋白基因 AcTMX3 的分子特征，该基因在 Arma chinensis diapause 的抗氧化防御中发挥重要作用。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-02-01 Epub Date: 2024-10-23 DOI: 10.1111/imb.12964

Qiaozhi Luo, Jianjun Mao, Yuyan Li, Mengqing Wang, Lisheng Zhang, Zhongjian Shen

{"title":"Molecular characterization of a novel thioredoxin-related transmembrane protein gene AcTMX3 that plays important roles in antioxidant defence in Arma chinensis diapause.","authors":"Qiaozhi Luo, Jianjun Mao, Yuyan Li, Mengqing Wang, Lisheng Zhang, Zhongjian Shen","doi":"10.1111/imb.12964","DOIUrl":"10.1111/imb.12964","url":null,"abstract":"Protein disulphide isomerase (PDI) possesses disulphide isomerase, oxidoreductase and molecular chaperone activities, and is involved in regulating various physiological processes. However, there are few studies on the function in insect diapause. In this study, we cloned one novel member PDI family (TMX3, thioredoxin-related transmembrane protein 3) in Arma chinensis. The AcTMX3 encodes 426 amino acids that contains a predicted N-terminal signal sequence, a thioredoxin-like domain with the CXXC active site and a potential transmembrane region, which are typical sequence features of TMX3. RT-qPCR results showed that AcTMX3 was mainly expressed in the head under non-diapause conditions, while AcTMX3 was highly expressed in the fat body (central metabolic organ) under diapause conditions. Moreover, temporal expression profile showed that compared with non-diapause conditions, diapause conditions significantly induced AcTMX3 expression, and the expression of AcTMX3 was enhanced at 15°C. Silencing AcTMX3 in A. chinensis significantly inhibited the expression of antioxidant genes (AcTrx2 and AcTrx-like), increased the content of H2O2 and ascorbate and reduced the survival rate of A. chinensis under diapause conditions. Our results suggested that AcTMX3 played an important role in the resistance of A. chinensis to oxidative stress under diapause conditions.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":"218-227"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tyrosine transfer RNA levels and modifications during blood-feeding and vitellogenesis in the mosquito, Aedes aegypti. 埃及伊蚊采血和卵黄发生过程中的酪氨酸转运核糖核酸水平和修饰。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-02-01 Epub Date: 2024-08-06 DOI: 10.1111/imb.12950

Melissa Kelley, Christopher J Holmes, Cassandra Herbert, Asif Rayhan, Judd Joves, Melissa Uhran, Lucas Klaus, Ronja Frigard, Khwahish Singh, Patrick A Limbach, Balasubrahmanyam Addepalli, Joshua B Benoit

{"title":"Tyrosine transfer RNA levels and modifications during blood-feeding and vitellogenesis in the mosquito, Aedes aegypti.","authors":"Melissa Kelley, Christopher J Holmes, Cassandra Herbert, Asif Rayhan, Judd Joves, Melissa Uhran, Lucas Klaus, Ronja Frigard, Khwahish Singh, Patrick A Limbach, Balasubrahmanyam Addepalli, Joshua B Benoit","doi":"10.1111/imb.12950","DOIUrl":"10.1111/imb.12950","url":null,"abstract":"Mosquitoes such as Aedes aegypti must consume a blood meal for the nutrients necessary for egg production. Several transcriptome and proteome changes occur post-blood meal that likely corresponds with codon usage alterations. Transfer RNA (tRNA) is the adapter molecule that reads messenger RNA codons to add the appropriate amino acid during protein synthesis. Chemical modifications to tRNA enhance codon decoding, improving the accuracy and efficiency of protein synthesis. Here, we examined tRNA modifications and transcripts associated with the blood meal and subsequent periods of vitellogenesis in A. aegypti. More specifically, we assessed tRNA transcript abundance and modification levels in the fat body at critical times post blood-feeding. Based on a combination of alternative codon usage and identification of particular modifications, we discovered that increased transcription of tyrosine tRNAs is likely critical during the synthesis of egg yolk proteins in the fat body following a blood meal. Altogether, changes in both the abundance and modification of tRNA are essential factors in the process of vitellogenin production after blood-feeding in mosquitoes.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":"65-80"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705514/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141893397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0