Insect Molecular Biology最新文献

PBAN regulates sex pheromone biosynthesis by Ca²⁺/CaN/ACC and Ca²⁺/PKC/HK2 signal pathways in Spodoptera litura. PBAN通过Ca2+/CaN/ACC和Ca2+/PKC/HK2信号途径调控鞘翅目昆虫性信息素的生物合成。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2024-11-16 DOI: 10.1111/imb.12976

Yao Zhang, Ruolan He, Zelong She, Xinming Yin, Xiang Li, Shuangyan Yao, Mengfang Du, Shiheng An

{"title":"PBAN regulates sex pheromone biosynthesis by Ca2+/CaN/ACC and Ca2+/PKC/HK2 signal pathways in Spodoptera litura.","authors":"Yao Zhang, Ruolan He, Zelong She, Xinming Yin, Xiang Li, Shuangyan Yao, Mengfang Du, Shiheng An","doi":"10.1111/imb.12976","DOIUrl":"https://doi.org/10.1111/imb.12976","url":null,"abstract":"Sex pheromones emitted by female moths play important roles in mate attraction. The molecular mechanism underlying pheromone biosynthesis activating neuropeptide (PBAN)-regulated sex pheromone biosynthesis has been well elucidated in many moth species, although this mechanism is species-dependent. Spodoptera litura, an important pest, has caused serious economic losses to agricultural production, yet the mechanism for its sex pheromone biosynthesis has not been fully identified. The present study investigates in detail mechanism underlying PBAN-regulated sex pheromone biosynthesis in S. litura. The transcriptome sequencing of S. litura pheromone glands (PGs) was analysed to identify a serial of candidate genes potentially involved in sex pheromone biosynthesis. Further investigation revealed a bimodal pattern in both sex pheromone release and mating frequency. PBAN was found to regulate sex pheromone biosynthesis via its receptor by using Ca2+ as a secondary messenger, as demonstrated by RNA interference and the application of pharmacological inhibitors. Furthermore, PBAN/Ca2+ signalling activated calcineurin (CaN) and acetyl-CoA carboxylase (ACC), which mediated sex pheromone biosynthesis in response to PBAN stimulation. Mostly importantly, hexokinase 2 (HK2) was confirmed to be activated by PBAN/PBANR /Ca2+/PKC signalling via phosphorylation at two specific sites (ser423 and ser434 sites of HK2). Overall, our findings shed light on the intricate processes involved in sex pheromone production in S. litura, in which PBAN regulates sex pheromone biosynthesis through PBAN/PBANR/Ca2+/CaN/ACC and PBAN/PBANR/Ca2+/PKC/HK2 signalling pathways. These insights significantly contribute to our comprehension of the specific mechanisms underlying sex pheromone biosynthesis in this moth species.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The JNK signalling pathway gene BmJun is involved in the regulation of egg quality and production in the silkworm, Bombyx mori. JNK信号通路基因BmJun参与了家蚕卵质量和产量的调控。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2024-11-14 DOI: 10.1111/imb.12975

Yu-Guo Wang, An-Qi Liu, Yasir Khan, Yi Zhang, Chen-Chen Wang, Yao-Le Song, Jiang-Han Du, Yang-Hu Sima, Jian-Feng Qiu, Shi-Qing Xu

{"title":"The JNK signalling pathway gene BmJun is involved in the regulation of egg quality and production in the silkworm, Bombyx mori.","authors":"Yu-Guo Wang, An-Qi Liu, Yasir Khan, Yi Zhang, Chen-Chen Wang, Yao-Le Song, Jiang-Han Du, Yang-Hu Sima, Jian-Feng Qiu, Shi-Qing Xu","doi":"10.1111/imb.12975","DOIUrl":"https://doi.org/10.1111/imb.12975","url":null,"abstract":"The Jun N-terminal kinase (JNK) signalling pathway has a key role in tissue remodelling during insect metamorphosis by regulating programmed cell death. However, multiple members of the JNK pathway in Lepidoptera remain uncharacterized. In this study, two key genes of the JNK pathway, BmJun and BmFos, were cloned from the silkworm Bombyx mori, a lepidopteran model insect, and their effects on reproductive development were investigated. BmJun and BmFos encode 239 and 380 amino acids, respectively. Both proteins have typical basic leucine zipper domains and form a BmJUN-BmFOS dimer activator protein to exert transcriptional regulation. During the wandering stage of silkworm development, interference in BmJun expression had no effect on pupation, whereas B. mori vitellogenin (BmVg) expression, which is essential for egg development, was suppressed in the fat body and egg laying was significantly reduced. Additionally, numerous eggs appeared shrivelled and deformed, suggesting that they were nutritionally stunted. Inhibition of the JNK pathway caused abnormal pupal metamorphosis, an increase in shrivelled, unfertilized eggs, a decrease in fat body synthesis, and accumulation of BmVg in the ovaries of female B. mori. The results indicated that BmJUN and BmFOS can form an AP-1 dimer. Interfering with BmJun or inhibiting the phosphorylation of BmJUN leads to a reduction in the synthesis of BmVg in the fat body and its accumulation in the ovaries, thereby affecting the quality and production of the progeny eggs. These findings suggest that regulating Jun in the JNK pathway could be a potential way to inhibit female reproduction in Lepidoptera.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142619381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pleiotropic effects of Ebony on pigmentation and development in the Asian multi-coloured ladybird beetle, Harmonia axyridis (Coleoptera: Coccinellidae). 乌木对亚洲多色瓢虫 Harmonia axyridis（鞘翅目：瓢虫科）色素沉着和发育的多效作用

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2024-11-08 DOI: 10.1111/imb.12968

Jing Lin, Da Xiao, Mengmeng Wu, Xu Chen, Qingxuan Xu, Su Wang, Liansheng Zang

{"title":"Pleiotropic effects of Ebony on pigmentation and development in the Asian multi-coloured ladybird beetle, Harmonia axyridis (Coleoptera: Coccinellidae).","authors":"Jing Lin, Da Xiao, Mengmeng Wu, Xu Chen, Qingxuan Xu, Su Wang, Liansheng Zang","doi":"10.1111/imb.12968","DOIUrl":"https://doi.org/10.1111/imb.12968","url":null,"abstract":"Melanin plays a pivotal role in insect body pigmentation, significantly contributing to their adaptation to diverse biotic and abiotic environmental challenges. Several genes involved in insect melanin synthesis showed pleiotropic effects on insect development and reproduction. Among these, the N-β-alanyl dopamine synthetase gene (Ebony) is integral to the pigmentation process. However, the full spectrum of its pleiotropic impacts is not yet thoroughly understood. In this study, we identified and characterised the HaEbony gene in the Asian multi-coloured ladybird beetle (Harmonia axyridis) and found that HaEbony gene is a conserved gene within the Coleoptera order. We aimed to further explore the multiple roles of HaEbony in the physiology and behaviour in H. axyridis. The CRISPR/Cas9 system was applied to generate multiple HaEbony knockout allele (HaEbony+/-), showing nucleotide deletion in the G0 and G1 generations. Remarkably, the resultant HaEbony+/- mutants consistently displayed darker pigmentation than their wild-type counterparts across larval, pupal and adult stages. Furthermore, these HaEbony+/- individuals (G0) demonstrated an enhanced predatory efficiency, evidenced by a higher number of aphids consumed compared to the wild type. A significant finding was the reduced egg hatchability in both G0 and G1 generations of the HaEbony+/- group, highlighting a potential reproductive fitness cost associated with HaEbony deficiency. In conclusion, our study not only sheds light on the multifaceted roles of HaEbony in H. axyridis but also highlights the potential of employing CRISPR/Cas9-targeted modifications of the Ebony gene. Such genetic interventions could enhance the environmental adaptability and predatory efficacy of ladybirds, presenting a novel strategy in biological control application.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validation of selective catalytic BmCBP inhibitors that regulate the Bm30K-24 protein expression in silkworm, Bombyx mori. 验证调节家蚕 Bm30K-24 蛋白表达的选择性催化 BmCBP 抑制剂

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2024-11-08 DOI: 10.1111/imb.12974

Jiasheng Geng, Weina Lu, Qinglong Kong, Jiao Lv, Yue Liu, Guowei Zu, Yanmei Chen, Caiying Jiang, Zhengying You, Zuoming Nie

{"title":"Validation of selective catalytic BmCBP inhibitors that regulate the Bm30K-24 protein expression in silkworm, Bombyx mori.","authors":"Jiasheng Geng, Weina Lu, Qinglong Kong, Jiao Lv, Yue Liu, Guowei Zu, Yanmei Chen, Caiying Jiang, Zhengying You, Zuoming Nie","doi":"10.1111/imb.12974","DOIUrl":"https://doi.org/10.1111/imb.12974","url":null,"abstract":"The cAMP response element binding protein (CREB)-binding protein (CBP) is a histone acetyltransferase that plays an indispensable role in regulating the acetylation of histone and non-histone proteins. Recently, it has been discovered that chemical inhibitors A485 and C646 can bind to Bombyx mori's CBP (BmCBP) and inhibit its acetyltransferase activity. Notably, the binding ability of A485 with BmCBP showed a very low Kd value of 48 nM by surface plasmon resonance (SPR) test. Further identification showed that both A485 and C646 can decrease the acetylation level of known substrate H3K27 and only 1 μM of A485 can almost completely inhibit the acetylation of H3K27, suggesting that A485 is an effective inhibitor of BmCBP's acetyltransferase activity. Moreover, it was confirmed that A485 could downregulate the expression of acetylated Bm30K-24 protein at a post-translational level through acetylation modification by BmCBP. Additionally, it was found that A485 can downregulate the stability of Bm30K-24 and improve its ubiquitination level, suggesting that the acetylation modification by BmCBP could compete with ubiquitination modification at the same lysine site on Bm30K-24, thereby affecting its protein stability. Here, we predict that A485 may be a potent CBP acetyltransferase inhibitor which could be utilized to inhibit acetyltransferase activity in insects, including silkworms.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Juvenile hormone controls trehalose metabolism by regulating trehalase 2 activity in ovarian development of Helicoverpa armigera. 幼年激素通过调节 Helicoverpa armigera 胚胎发育过程中曲卤糖酶 2 的活性来控制曲卤糖代谢。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2024-11-06 DOI: 10.1111/imb.12969

Qianyue Qin, Bo Zhang, Bin Fang, Yanpeng Chang, Xiang Li, Shiheng An, Wenli Zhao

{"title":"Juvenile hormone controls trehalose metabolism by regulating trehalase 2 activity in ovarian development of Helicoverpa armigera.","authors":"Qianyue Qin, Bo Zhang, Bin Fang, Yanpeng Chang, Xiang Li, Shiheng An, Wenli Zhao","doi":"10.1111/imb.12969","DOIUrl":"https://doi.org/10.1111/imb.12969","url":null,"abstract":"Trehalase (Treh) is crucial for ovarian development as it directly regulates the energy supply by hydrolyzing trehalose into glucose. Juvenile hormone (JH) is also essential for ovarian development, but how it affects Treh2 activity remains unclear. This study, which employed Helicoverpa armigera as a model, showed that HaTreh2 transcription and enzymatic activity peaks coincided with the peak of JH titers (the 2 and 3 days after emergence). Compared to the dsGFP control, knockdown of HaTreh2 transcription severely impaired ovarian development. LC-MS/MS and site mutation experiments demonstrated that JH triggered the serine 345 phosphorylation of HaTreh2 via the GPCR-cAMP-PKA pathway, thereby activating its enzymatic activity. Additionally, HaTreh2 is directly bound with trehalose transporter (HaTreT) under JH induction, thus controlling intracellular trehalose and glucose contents as well as the transcription of HaTreT. TreT controls the amount of trehalose, which serves as a substrate for Treh1, entering the cell. Treh2, on the other hand, uses extracellular trehalose as substrate, and the hydrolysis product glucose is further transported into the cell. Here, HaTreh2 regulated the substrate that HaTreh1 can act upon in the cell by directly binding with HaTreT during ovarian development when JH is induced. Therefore, JH systematically regulated trehalose metabolism during ovarian development through regulating the activity of HaTreh2. This study sheds light on the coordinated interplay between JH pathway and sugar metabolism in ovarian development.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host trees partially explain the complex bacterial communities of two threatened saproxylic beetles. 寄主树部分解释了两种濒危甲虫复杂的细菌群落。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2024-11-06 DOI: 10.1111/imb.12973

Michał Kolasa, Rama Sarvani Krovi, Radosław Plewa, Tomasz Jaworski, Marcin Kadej, Adrian Smolis, Jerzy M Gutowski, Krzysztof Sućko, Rafał Ruta, Tomasz Olbrycht, Sergey Saluk, Maria Oczkowicz, Łukasz Kajtoch

{"title":"Host trees partially explain the complex bacterial communities of two threatened saproxylic beetles.","authors":"Michał Kolasa, Rama Sarvani Krovi, Radosław Plewa, Tomasz Jaworski, Marcin Kadej, Adrian Smolis, Jerzy M Gutowski, Krzysztof Sućko, Rafał Ruta, Tomasz Olbrycht, Sergey Saluk, Maria Oczkowicz, Łukasz Kajtoch","doi":"10.1111/imb.12973","DOIUrl":"https://doi.org/10.1111/imb.12973","url":null,"abstract":"Microorganisms are integral to ecosystem functioning and host adaptation, yet the understanding of microbiomes in diverse beetle taxa remains limited. We conducted a comprehensive study to investigate the microbial composition of two red flat bark beetle species, Cucujus haematodes and C. cinnaberinus, and assessed the influence of host taxonomic relatedness and host tree species on their microbiomes. We sampled 67 larvae of two Cucujus taxa taken from 11 host tree species. 16S rRNA V4 fragment sequencing revealed distinct microbial communities associated with each Cucujus species, with host tree species significantly influencing microbiome composition. Alpha and beta diversity metrics indicated significant differences between microbial communities in both beetle and host tree species. Principal component analysis indicated distinct clustering based on host tree species but not for beetle species. This overlap could be attributed to the similar ecology of both Cucujus species. The detection of various bacteria, among which some have already been reported in saproxylophagous beetles, suggests that the red flat bark beetles ingest the bacteria via foraging on other wood-dwelling invertebrates. Our findings show the complex interplay between host taxonomy, microhabitat and microbial composition in Cucujus, providing insights into their ecological roles and conservation implications. This research helps to fill the gap in understanding the microbial dynamics of saproxylic beetles, sheds light on factors shaping their microbiomes and highlights the importance of considering both host species and environmental conditions when studying insect-microbe interactions in forest ecosystems.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NPF and sNPF can regulate the feeding behaviour and affect the growth and antioxidant levels of the rice brown planthopper, Nilaparvata lugens. NPF 和 sNPF 可调节稻褐飞虱的取食行为，并影响其生长和抗氧化剂水平。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2024-11-04 DOI: 10.1111/imb.12971

Rui-Chuan Duan, Yu-Ning Zhang, Yan-Hui Wang, Bo-Xuan Xie, Zheng-Ze Du, Fa-Jun Chen

{"title":"NPF and sNPF can regulate the feeding behaviour and affect the growth and antioxidant levels of the rice brown planthopper, Nilaparvata lugens.","authors":"Rui-Chuan Duan, Yu-Ning Zhang, Yan-Hui Wang, Bo-Xuan Xie, Zheng-Ze Du, Fa-Jun Chen","doi":"10.1111/imb.12971","DOIUrl":"https://doi.org/10.1111/imb.12971","url":null,"abstract":"Neuropeptide F (NPF) and short neuropeptide F (sNPF) are important neuropeptides and mainly affect feeding behaviour of insects. However, the regulation of insect feeding behaviour by NPF and sNPF appears to differ between species, and it is not clear how NPF and sNPF regulate the food intake of the brown planthopper (Nilaparvata lugens). Therefore, the functions of NPF and sNPF in regulating food intake and affecting the growth and antioxidant levels of N. lugens fed on host rice plants were investigated by knocking down NPF and sNPF respectively and simultaneously knocking down both of them by RNA interference. The results showed that NPF and sNPF were mainly expressed in the head of N. lugens, and N. lugens increased food intake after NPF and sNPF were knocked down, which was reflected in the prolonged duration of N4a and N4b waves in the electrical penetration graph (EPG) experiment after knocking down NPF and sNPF. In addition, knocking down NPF and sNPF led to the increase of body weight and mortality of N. lugens, and also led to the increase of antioxidant level of N. lugens. So it was concluded that NPF and sNPF could regulate food intake, maintain body weight stability and oxidative balance in N. lugens. Our study clarified the molecular mechanism of NPF and sNPF regulating feeding behaviour and affect the growth and antioxidant level of N. lugens.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of TRPV channels in texture discrimination during Bactrocera dorsalis egg-laying behaviour. TRPV 通道在 Bactrocera dorsalis 产卵行为中的质地辨别中的作用。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2024-10-31 DOI: 10.1111/imb.12970

Zhong-Nian Huang, Feng-Yi Guo, Zi-Yuan Li, Yong-Yue Lu, Yi-Xiang Qi

{"title":"Role of TRPV channels in texture discrimination during Bactrocera dorsalis egg-laying behaviour.","authors":"Zhong-Nian Huang, Feng-Yi Guo, Zi-Yuan Li, Yong-Yue Lu, Yi-Xiang Qi","doi":"10.1111/imb.12970","DOIUrl":"https://doi.org/10.1111/imb.12970","url":null,"abstract":"Deciding where to lay an egg is critical for the survival of insects' offspring. Compared with our understanding of the chemosensory assessment of egg-laying sites, the mechanisms of texture detection are largely unknown. Here, we show that Bactrocera dorsalis, a notoriously agricultural pest laying its eggs within ripening fruits, can discriminate substrate texture during the egg-laying process. Exposure to drugs targeting transient receptor potential vanilloid (TRPV) mechanosensory channels abolished their oviposition preference for hard textures. BdorNan and BdorIav are two members of the TRPV subfamily, and their transcripts were detected in the labellum, the foreleg tarsi and the ovipositor. Then, we successfully obtained knockout strains of each gene using the CRISPR/Cas9 technique. The results showed that BdorNan is required for the discrimination of stiffness difference. BdorIav knockout had no significant effect on the ability of B. dorsalis to choose harder substrates. Our study thus reveals that BdorNan plays a substantial role in the texture assessment of egg-laying behaviour in B. dorsalis.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GC-MS-based metabonomic analysis of silkworm haemolymph reveals four-stage metabolic responses to nucleopolyhedrovirus infection. 基于气相色谱-质谱（GC-MS）的家蚕血淋巴代谢分析揭示了家蚕感染核多角体病毒后的四阶段代谢反应。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2024-10-31 DOI: 10.1111/imb.12972

Zhenyue Su, Yi Li, Zihan Lin, Qing Huang, Xinyu Fan, Zhaoming Dong, Qingyou Xia, Ping Zhao, Xin Wang

{"title":"GC-MS-based metabonomic analysis of silkworm haemolymph reveals four-stage metabolic responses to nucleopolyhedrovirus infection.","authors":"Zhenyue Su, Yi Li, Zihan Lin, Qing Huang, Xinyu Fan, Zhaoming Dong, Qingyou Xia, Ping Zhao, Xin Wang","doi":"10.1111/imb.12972","DOIUrl":"https://doi.org/10.1111/imb.12972","url":null,"abstract":"Silkworm, Bombyx mori, an economically significant insect, plays a crucial role in silk production. However, silkworm breeding is highly susceptible to various pathogens, particularly the Bombyx mori nucleopolyhedrovirus (BmNPV), which poses a serious threat. Recent metabonomic studies have provided insights into the metabolic changes associated with BmNPV infection. BmNPV infection has obvious temporal characteristics. However, few studies have investigated the silkworms infected in different periods. This study employed gas chromatography-mass spectrometry (GC-MS) to perform a comprehensive analysis of haemolymph metabolites in silkworms at 48, 72, 96 and 120 h post-infection (h.p.i.). Through the integration of time-course analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, the study revealed distinct four-stage metabolic characteristics in the silkworm's response to BmNPV infection. At Stage 1 (48 h.p.i.), silkworms activate antioxidant defence mechanisms, with significant enrichment in metabolic pathways involving key antioxidants such as glutathione, to mitigate oxidative stress induced by viral invasion. By Stage 2 (72 h.p.i.), pathways related to amino acid metabolism and protein synthesis become active, indicating an increase in protein synthesis. In Stage 3 (96 h.p.i.), energy metabolism and substance transport pathways are significantly upregulated to support the rapid viral replication and the enhanced locomotor behaviour of silkworm. Finally, at Stage 4 (120 h.p.i.), there is a further enhancement of pathways related to energy metabolism, nucleic acid synthesis, and substance transport, which align with peak viral assembly and release. These findings contribute to an in-depth understanding of the biochemical basis of silkworm resistance to NPV.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockdown of tyrosine hydroxylase gene affects larval survival, pupation and adult eclosion in Plagiodera versicolora. 敲除酪氨酸羟化酶基因会影响 Plagiodera versicolora 的幼虫存活、化蛹和成虫羽化。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2024-10-29 DOI: 10.1111/imb.12967

Xiaolong Liu, Xin Wang, Qi Zhang, Longji Ze, Hainan Zhang, Min Lu

引用次数: 0