Insect Molecular Biology最新文献

Histone and N-terminal acetyltransferases play important roles in female reproduction and embryogenesis of the red flour beetle Tribolium castaneum. 组蛋白和n端乙酰转移酶在红粉甲虫雌性生殖和胚胎发生中起重要作用。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-05-29 DOI: 10.1111/imb.13000

Karthi Sengodan, Subba Reddy Palli

{"title":"Histone and N-terminal acetyltransferases play important roles in female reproduction and embryogenesis of the red flour beetle Tribolium castaneum.","authors":"Karthi Sengodan, Subba Reddy Palli","doi":"10.1111/imb.13000","DOIUrl":"https://doi.org/10.1111/imb.13000","url":null,"abstract":"Histone acetyltransferases (HATs) catalyse the addition of acetyl groups to histones and other proteins. In contrast, histone deacetylases remove acetyl groups from core histones, and the activity of these enzymes maintains the acetylation levels of these proteins. Histone acetylation levels influence chromatin accessibility and gene expression and regulate many biological processes, including development and reproduction. Recent reports suggest that some N-terminal acetyltransferases (NATs) also regulate gene expression. We identified 29 HAT and NAT genes in the red flour beetle, Tribolium castaneum, and studied their functions in female reproduction using RNA interference (RNAi). Knockdown of seven out of 13 HAT genes (N-acetyltransferase ESCO2) (ESCO1/2), Elongator complex protein 3 (ELP3), Histone acetyltransferase type B catalytic subunit 1 (HAT1), Transcription initiation factor TFIID subunit 1 (TAF1), Protein x-mas-2 (MCM3AP), Histone acetyltransferase Tip60 (KAT5), and Cysteine-rich protein 2-binding protein (KAT14) and 12 out of 16 NAT genes Probable glucosamine 6-phosphate N-acetyltransferase (GNPNAT1), N-alpha-acetyltransferase 10 (NAA10), N-alpha-acetyltransferase 20 (NAA20), N-alpha-acetyltransferase 30 (NAA30), N-alpha-acetyltransferase 40 (NAA40), N-alpha-acetyltransferase 60 (NAA60), N-acetyltransferase 6 (NAA80), RNA cytidine acetyltransferase (NAT10), Diamine acetyltransferase 2 (SATL1), N(alpha)-acetyltransferase 16 (NAA16), Phagocyte signalling-impaired protein (NAA25), N(alpha)-acetyltransferase 35 (NAA35) caused a significant reduction in eggs laid by females compared to the eggs laid by control females injected with dsGFP. Also, knockdown of nine (KAT5, ATAT1, ELP3, HAT1, KAT8A, NAA10, NAA20, GNPNAT1 and TAF1) HAT/NAT genes caused a significant decrease in egg hatching. Parental RNAi of ATAT1 and KAT8 blocked embryogenesis. These data suggest that the acetylation of proteins plays an important role in female reproduction and embryogenesis.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144173611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temperature-dependent alternative splicing affects gene expression in Aedes aegypti mosquitoes midgut. 温度依赖性选择性剪接影响埃及伊蚊中肠基因表达。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-05-29 DOI: 10.1111/imb.13002

Priscila Gonçalves Ferreira, Miqueias Fernandes, Camila Aparecida Profeta, Renata Cristina Barbosa, Courtney Cuinn Murdock, Gustavo Ferreira Martins, Tiago de Oliveira Mendes

{"title":"Temperature-dependent alternative splicing affects gene expression in Aedes aegypti mosquitoes midgut.","authors":"Priscila Gonçalves Ferreira, Miqueias Fernandes, Camila Aparecida Profeta, Renata Cristina Barbosa, Courtney Cuinn Murdock, Gustavo Ferreira Martins, Tiago de Oliveira Mendes","doi":"10.1111/imb.13002","DOIUrl":"https://doi.org/10.1111/imb.13002","url":null,"abstract":"Temperature is one of the most significant abiotic factors influencing vector resistance to pathogens. Research has provided substantial insights into the immunological and oxidative processes affected by temperature. However, our understanding of the complex interplay in mosquito-pathogen interactions remains limited. In this study, we investigated the impact of temperature on alternative splicing in the midgut of Aedes aegypti mosquitoes (adult females) under different thermal conditions. Few genes exhibited differential alternative splicing when comparing low (20°C) and high (36°C) temperatures to standard rearing conditions (28°C). Among these, Trypsin (TRY), Ferritin (FER), Thioredoxin (TRX) and Peptidoglycan recognition protein LC (PGRP-LC) were identified, aligning with previous findings that their expression is temperature-sensitive. Among the genes identified with alternative splicing, we focus on Thioredoxin and PGRP-LC, genes modulated by temperature variations and promising targets for future studies on their role in the competence of Ae. aegypti to transmit Zika. Experimental validation confirmed that TRX, a gene critical for pathogen defence, has a differentially spliced exon under warmer conditions, potentially altering its activity. In contrast, no differential splicing was observed for PGRP-LC across temperature treatments. These findings suggest that temperature-induced alternative splicing may play a role in shaping the mosquito's physiological responses to environmental changes, highlighting a previously underexplored layer of complexity in mosquito-pathogen dynamics.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144173612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PBX and Dfd cooperatively regulate stage-specific expression of 30 K protein BmLP1 in Bombyx mori. PBX和Dfd共同调控家蚕30k蛋白BmLP1的分期特异性表达。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-05-27 DOI: 10.1111/imb.12998

Qingqing Linghu, Haoyun Li, Jiahui Wan, Xiaolu Zhang, Jingjing Huang, Zhiqing Li, Yuying Wang, Jianhua Xia, Zhaoming Dong, Ying Lin, Ping Zhao, Yan Zhang

{"title":"PBX and Dfd cooperatively regulate stage-specific expression of 30 K protein BmLP1 in Bombyx mori.","authors":"Qingqing Linghu, Haoyun Li, Jiahui Wan, Xiaolu Zhang, Jingjing Huang, Zhiqing Li, Yuying Wang, Jianhua Xia, Zhaoming Dong, Ying Lin, Ping Zhao, Yan Zhang","doi":"10.1111/imb.12998","DOIUrl":"https://doi.org/10.1111/imb.12998","url":null,"abstract":"Nutrient accumulation is essential for insect metamorphosis. As a group of important nutrient-storage proteins, forty-six 30 K proteins (30KPs), including BmLP1-BmLP46, have been identified in the silkworm, Bombyx mori. Most 30KPs are synthesised in the last instar larvae, and the stage-specific expression of 30KPs is believed to be regulated by juvenile hormone (JH)-dependent pathways; however, the specific regulatory mechanism remains unclear. In this study, we found that a 30KP gene Bmlp1 was expressed after Day 3 of the fifth instar, and its expression was down-regulated by JH analogue. We also identified a cis-response element (CRE) on the promoter of Bmlp1. Dfd was determined to bind to this CRE adjacent to another CRE that serves as a binding site for PBX. Dfd is a HOX transcription factor found to exhibit an expression pattern similar to that of PBX. The interaction between PBX and Dfd was confirmed using bimolecular fluorescence complementation and GST pull-down experiments. The expression of Bmlp1 was down-regulated when PBX and Dfd were overexpressed in BmN cells, whereas it was up-regulated when PBX and Dfd were knocked down in BmN cells. Our data show that the transcription factor Dfd, and the cofactor PBX, synergistically regulate the transcription of Bmlp1 in B. mori. This study provides a reference for an in-depth understanding of the regulation of insect development mediated by JH.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism of Ca²⁺ in regulating pupation defects of Bombyx mori after exposure to chlorantraniliprole. 氯虫腈暴露后Ca2+调控家蚕化蛹缺陷的机制

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-05-26 DOI: 10.1111/imb.12999

Jialu Cheng, Haoyi Gu, Hongbin Zou, Xiaoxia Zhang, Peiling Peng, Xueling Qin, Bing Li

引用次数: 0

Environmental factors affecting RNAi efficacy: Temperature but not plant cultivar influences Colorado potato beetle's response to insecticidal dsRNA. 影响RNAi效果的环境因素：影响科罗拉多马铃薯甲虫对杀虫dsRNA反应的因素是温度，而不是植物品种。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-05-23 DOI: 10.1111/imb.12996

Mike Darrington, Jason Solocinski, Sophia K Zhou, Melise C Lecheta, Subba Reddy Palli, Yolanda H Chen, Nicholas M Teets

{"title":"Environmental factors affecting RNAi efficacy: Temperature but not plant cultivar influences Colorado potato beetle's response to insecticidal dsRNA.","authors":"Mike Darrington, Jason Solocinski, Sophia K Zhou, Melise C Lecheta, Subba Reddy Palli, Yolanda H Chen, Nicholas M Teets","doi":"10.1111/imb.12996","DOIUrl":"https://doi.org/10.1111/imb.12996","url":null,"abstract":"Environmental RNAi (eRNAi) is a recent innovation in insect pest control, and comprehensive risk assessment is needed to ensure the environmental safety and longevity of this technology. As eRNAi relies on the insect's cellular machinery for its mode of action, environmentally mediated plasticity in the activity of cellular processes required for RNAi could influence efficacy and the development of resistance. Here, we investigated the extent to which plant cultivar and temperature influence the efficacy of insecticidal double-stranded RNA (dsRNA) targeting actin in larvae of the Colorado potato beetle, Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae). Potato cultivar did not significantly affect survival or gene silencing in dsRNA-treated larvae, indicating that efficacy is consistent across potato varieties, at least under laboratory conditions. Temperature did influence RNAi efficacy, with both gene silencing and mortality being reduced when dsRNA treatment occurred at lower temperatures. After 3 days of feeding with dsRNA, gene silencing occurred at all temperatures, but knockdown efficiency was 62% at 30°C and 35% at 18°C. eRNAi efficacy at different temperatures was not related to transcript levels of core RNAi genes, indicating that other mechanisms are responsible for the observed effects. Overall, these results indicate that environmental conditions can influence the efficacy of insecticidal eRNAi and may affect the rate at which insects develop resistance to these technologies.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual oxidase is essential for moulting, hatching and feeding in the brown planthopper. 双氧化酶是褐飞虱蜕皮、孵化和取食的必需酶。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-05-22 DOI: 10.1111/imb.12995

Jinjin Ren, Shuai Tao, Xu Cheng, Yanyuan Bao

引用次数: 0

HcTRET1 is critical for epidermal chitin synthesis in Hyphantria cunea. HcTRET1对加利福尼亚棘球绦虫表皮几丁质合成至关重要。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-05-09 DOI: 10.1111/imb.12994

Diankuan Liu, Chuanshan Zou, Shengyu Zhang, Ze Wang, Jinxin Yu, Yuyao Nan, Zixin Dong

{"title":"HcTRET1 is critical for epidermal chitin synthesis in Hyphantria cunea.","authors":"Diankuan Liu, Chuanshan Zou, Shengyu Zhang, Ze Wang, Jinxin Yu, Yuyao Nan, Zixin Dong","doi":"10.1111/imb.12994","DOIUrl":"https://doi.org/10.1111/imb.12994","url":null,"abstract":"In insects, trehalose is critical for growth and development, as well as environmental stress response, which is mainly transported by trehalose transporters (TRETs). Over nearly two decades, the physiological functions of TRETs in insect growth, development, reproduction and environmental stress response have been well elucidated. However, the role of TRETs in chitin synthesis remains not fully understood. Here, we identified the HcTRET1 gene from Hyphantria cunea, a major Lepidoptera pest in agriculture and forestry. The role of HcTRET1 in growth and development, especially in chitin synthesis, was discussed by dsRNA-mediated HcTRET1 knockdown. Bioassay showed that HcTRET1 knockdown did not affect larval growth, development and survival in H. cunea, but it significantly reduced the pupa formation rate. Additionally, HcTRET1 silencing increased trehalose levels in the fat body but decreased them in the hemolymph, suggesting HcTRET1 plays a key role in trehalose homeostasis. Moreover, HcTRET1 knockdown significantly downregulated the genes for chitin synthesis (HcGFAT, HcUAP and HcCHSA), resulting in a remarkable reduction of chitin content in the epidermis. Moreover, HcTRET1 knockdown significantly reduced the survival of H. cunea larvae at 42°C. Taken together, these results demonstrated that HcTRET1 played a critical role in larva-pupa transition, in vivo trehalose homeostasis, especially in epidermal chitin biosynthesis in H. cunea larvae. In parallel, its important physiological function in response to high-temperature stress has been verified as well. The findings expand our understanding of the physiological functions of TRET1 in insects, providing a new perspective for trehalose transporters to regulate chitin biosynthesis.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-omics analysis reveals discordant proteome and transcriptome responses in larval guts of Frankliniella occidentalis infected with an orthotospovirus. 多组学分析显示，感染一种正形体病毒的西富兰克林线虫幼虫肠道中的蛋白质组和转录组反应不一致。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-04-25 DOI: 10.1111/imb.12992

Jinlong Han, Dorith Rotenberg

{"title":"Multi-omics analysis reveals discordant proteome and transcriptome responses in larval guts of Frankliniella occidentalis infected with an orthotospovirus.","authors":"Jinlong Han, Dorith Rotenberg","doi":"10.1111/imb.12992","DOIUrl":"https://doi.org/10.1111/imb.12992","url":null,"abstract":"The western flower thrips, Frankliniella occidentalis, is the principal thrips vector of Orthotospovirus tomatomaculae (order Bunyavirales, family Tospoviridae), a devastating plant-pathogenic virus commonly referred to as tomato spotted wilt virus (TSWV). The larval gut is the gateway for virus transmission by F. occidentalis adults to plants. In a previous report, gut expression at the transcriptome level was subtle but significant in response to TSWV in L1s. Since it has been well documented that the relationship between the expression of mRNA and associated protein products in eukaryotic cells is often discordant, we performed identical, replicated experiments to identify and quantify virus-responsive larval gut proteins to expand our understanding of insect host response to TSWV. While we documented statistically significant, positive correlations between the abundance of proteins (4189 identified) and their cognate mRNAs expressed in first and second instar guts, there was virtually no alignment of individual genes identified to be differentially modulated by virus infection at the transcriptome and proteome levels. Predicted protein-protein interaction networks associated with clusters of co-expressed proteins revealed wide variation in correlation strength between protein and cognate transcript abundance, which appeared to be associated with the type of cellular processes, cellular compartments and network connectivity represented by the proteins. In total, our findings indicate distinct and dynamic regulatory mechanisms of transcript and protein abundance (expression, modifications and/or turnover) in virus-infected gut tissues. This study provides molecular candidates for future functional analysis of thrips vector competence and underscores the necessity of examining complex virus-vector interactions at a systems level.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The genome-wide characterisation of cold shock proteins and prominent roles involved in cold response by configuring metabolic pathways in Haemaphysalis longicornis. 冷休克蛋白的全基因组特征和通过配置长角血蜱的代谢途径参与冷反应的突出作用。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-04-25 DOI: 10.1111/imb.12993

Tingwei Pei, Ziwen Gao, Zihao Wang, Han Wang, Chuks F Nwanade, Ziyan Bing, Lu Li, Xiujie Liang, Yuchao Zhang, Yunsheng Tang, Xiaoduan Fang, Zhijun Yu

{"title":"The genome-wide characterisation of cold shock proteins and prominent roles involved in cold response by configuring metabolic pathways in Haemaphysalis longicornis.","authors":"Tingwei Pei, Ziwen Gao, Zihao Wang, Han Wang, Chuks F Nwanade, Ziyan Bing, Lu Li, Xiujie Liang, Yuchao Zhang, Yunsheng Tang, Xiaoduan Fang, Zhijun Yu","doi":"10.1111/imb.12993","DOIUrl":"https://doi.org/10.1111/imb.12993","url":null,"abstract":"Cold shock proteins are relatively conserved in evolution and are involved in regulating various life activities, including cell proliferation, nutritional stress and cold adaptation. However, information about the function and regulation of cold shock proteins in ticks during cold response remains meagre. In the present study, six cold shock protein genes were identified from the important vector tick Haemaphysalis longicornis, which were named as HlY-box1, HlY-box2, HlY-box3, HlY-box4, HlY-box5 and HlY-box6. Spatiotemporal expression dynamics revealed dynamic expressions varied significantly after low-temperature treatment, with different expression patterns observed over prolonged exposure periods. Then the function and regulation of cold shock protein genes during the cold response of H. longicornis were explored. RNA interference (RNAi) efficiently knocked down these genes, significantly increasing tick mortality under cold stress. Transcriptomic analysis following HlY-box4 knockdown identified 336 differentially expressed genes (DEGs), which were mainly annotated in the MAPK signalling pathway and metabolism pathway. Proteomic analysis identified 632 differentially expressed proteins associated with ATP-dependent chromatin remodelling, metabolic pathway, spliceosome, ribosome and nucleoplasmic transport pathways. The results highlight the critical roles of cold shock proteins (CSPs) in tick cold responses, primarily through regulating metabolic pathways, and provide a foundation for further exploration of their molecular mechanisms.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockdown of BmorCPR67 gene disrupts prepupal-pupal transition of silkworm Bombyx mori by thinning the endocuticle. 敲低BmorCPR67基因通过减薄家蚕的胚囊来破坏蛹前向蛹过渡。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-03-27 DOI: 10.1111/imb.12991

Mou-Zheng Jiang, Xiao-Xuan Hu, Xu-Dong Li, Qing Wang, Man-Yu Shi, Rui-Rui Cui, Guo-Qing Wei, Lei Wang

{"title":"Knockdown of BmorCPR67 gene disrupts prepupal-pupal transition of silkworm Bombyx mori by thinning the endocuticle.","authors":"Mou-Zheng Jiang, Xiao-Xuan Hu, Xu-Dong Li, Qing Wang, Man-Yu Shi, Rui-Rui Cui, Guo-Qing Wei, Lei Wang","doi":"10.1111/imb.12991","DOIUrl":"https://doi.org/10.1111/imb.12991","url":null,"abstract":"The cuticle of insects serves as a crucial organ for preserving body composition, protecting against pathogen invasion, and retaining moisture in their bodies. Cuticular proteins (CPs) are the main constituents of insect cuticles and interact with chitin to form the cuticle's structural framework and mechanical properties. In this study, we investigated the role of a cuticular protein with R&R consensus (CPR), BmorCPR67, a member of the RR-2 subfamily, during the prepupal-to-pupal transition in Bombyx mori. The BmorCPR67 gene exhibited high expression levels during the prepupal stage, with the highest expression detected in the epidermis of the day-1 pupa in B. mori. The expression of the BmorCPR67 gene was induced by 20-hydroxyecdysone (20E). Chitin-binding assays indicated that the BmorCPR67 protein selectively binds to crystalline chitin and chitosan but not to amorphous chitin. Silencing the BmorCPR67 gene disrupted the moulting process from prepupa to pupa, resulting in silkworm mortality. Furthermore, the knockdown of BmorCPR67 altered the expression profiles of key genes involved in chitin metabolism. Notably, significant thinning of the endocuticle was observed 48-96 h after siRNA injection in BmorCPR67-silenced silkworms. These findings highlight the critical role of BmorCPR67 in cuticle development during the prepupal-to-pupal transition in B. mori, contributing to our understanding of the functions of CPs in insect metamorphosis.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0