Insect Molecular Biology最新文献

Whole-genome resequencing reveals genetic evolution of honeybees (Apis cerana) in high-altitude and overwintering adaptation. 全基因组重测序揭示了蜜蜂（Apis cerana）在高海拔和越冬适应方面的遗传进化。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-06-20 DOI: 10.1111/imb.13009

Fugui Li, Xujiang He, Lizhen Zhang, Junfeng Liu, Zilong Wang, Zhijiang Zeng

{"title":"Whole-genome resequencing reveals genetic evolution of honeybees (Apis cerana) in high-altitude and overwintering adaptation.","authors":"Fugui Li, Xujiang He, Lizhen Zhang, Junfeng Liu, Zilong Wang, Zhijiang Zeng","doi":"10.1111/imb.13009","DOIUrl":"https://doi.org/10.1111/imb.13009","url":null,"abstract":"Over time, populations of Apis cerana across diverse geographical regions have undergone distinct phenotypic adaptations in response to varied climates, arising from prolonged natural evolutionary processes. Understanding the molecular genetic underpinnings and the implications of climate change on the adaptive evolution of A. cerana is crucial for its conservation amidst environmental shifts. Here, we conducted a comprehensive analysis of 110 bee individuals sourced from 11 regions at comparable temperatures, environments, and altitudes. A total of 525.56 Gb of filter-trimmed sequencing data were utilised for population genetic analysis of honeybees. The findings indicate genetic differentiation among bee populations from various geographical regions, with higher levels of population divergence observed in different altitudinal environments. Particularly noteworthy is the significant genetic divergence of the bee population from Haikou (HK) compared to populations in other areas, characterised by higher inbreeding coefficients and runs of homozygosity (ROHs), as well as lower nucleotide diversity. However, populations from Guangzhou (GZ), Jinzhai (JZ), Qimen (QM), Shennongjia (SNJ), and Suzhou (SZ) exhibit a close genetic relationship. Using comparisons across multiple bee populations from different groups, we identified selective signatures across different environmental temperatures and altitudes. Furthermore, by integrating genomic selection signals with comparative transcriptomic analysis, we identified genes potentially involved in bee adaptation to high altitude, such as g9950.t1 (alcohol dehydrogenase), g5267.t1 (diacylglycerol kinase theta-like isoform 2), gene g4025.t1 (Tyrosine 3-monooxygenase), and g3609.t1 (heme oxygenase). Our results indicate that both temperature and altitude contribute to the genetic differentiation and geographical adaptability of A. cerana populations.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and functional characterisation of a novel antimicrobial peptide from the housefly, Musca domestica. 家蝇一种新型抗菌肽的鉴定和功能表征。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-06-19 DOI: 10.1111/imb.13005

Dongdong Lin, Shuangshuang Li, Shiying Li, Ting Tang, Fengsong Liu

{"title":"Identification and functional characterisation of a novel antimicrobial peptide from the housefly, Musca domestica.","authors":"Dongdong Lin, Shuangshuang Li, Shiying Li, Ting Tang, Fengsong Liu","doi":"10.1111/imb.13005","DOIUrl":"https://doi.org/10.1111/imb.13005","url":null,"abstract":"Antimicrobial peptides (AMPs) are increasingly being recognised as promising alternatives to conventional antibiotics due to their distinctive antimicrobial mechanisms and reduced likelihood of inducing drug resistance. Insects represent a significant source of AMPs. In this study, a potential AMP gene, MdAMP5, was identified based on its strong immunoinducibility and the presence of a signal peptide, and an amphipathic α-helix in the encoded protein. MdAMP5 encoded a 50-amino acid precursor protein with an N-terminal 22-amino acid signal peptide. The calculated molecular mass of the mature protein was 2.92 kDa, with an estimated isoelectric point of 6.23. Structural analyses revealed that the N-terminus of mature MdAMP5 contained an irregularly coiled segment, while the C-terminus featured an amphipathic α-helix with a glycine-lysine residue at the end. Furthermore, the MdAMP5 gene was successfully expressed in the yeast Pichia pastoris, and the recombinant MdAMP5 (rMdAMP5) protein exhibited effective and broad-spectrum antimicrobial activity against both Gram-positive and Gram-negative bacteria in vitro and in vivo. Treatment with rMdAMP5 resulted in significant changes in bacterial morphology, including cell lysis and deformation of bacteriophages. In conclusion, this study identified and successfully expressed a novel AMP that showed low cytotoxicity to mammalian cells and high selectivity towards bacterial cells. This research offers a new candidate for therapeutic drug development, and enhances the understanding of the mechanism and application of AMPs.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolome-microbiome modulations induced by ZIF8@ZIF67 supplementation in the midgut of Bombyx mori. 添加ZIF8@ZIF67诱导家蚕中肠代谢组-微生物组的调节。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-06-13 DOI: 10.1111/imb.13008

Samreen Sadiq, Zhen-Yu Shen, Xinhao Jiao, Na Zhang, Lulai Wang, Tao Xu, Ping Wu, Iltaf Khan

{"title":"Metabolome-microbiome modulations induced by ZIF8@ZIF67 supplementation in the midgut of Bombyx mori.","authors":"Samreen Sadiq, Zhen-Yu Shen, Xinhao Jiao, Na Zhang, Lulai Wang, Tao Xu, Ping Wu, Iltaf Khan","doi":"10.1111/imb.13008","DOIUrl":"https://doi.org/10.1111/imb.13008","url":null,"abstract":"Bombyx mori is an economically crucial lepidopteran insect, and its health is closely related to the gut microbiota and metabolic status. Our previous research confirmed that metal-organic frameworks nanocomposite ZIF8@ZIF67 supplementation can significantly enhance silkworm survival rates upon bacterial challenges without adversely affecting its growth or cocoon weight. To elucidate the mechanism, this study integrated non-targeted metabolomics and 16S rRNA sequencing to dissect ZIF8@ZIF67-driven midgut modulations. The results showed that ZIF8@ZIF67 supplementation led to significant metabolic shifts in the midgut of B. mori, with 96 metabolites up-regulated and 54 down-regulated in the negative ion model, and 117 up-regulated and 43 down-regulated in the positive ion model. Key pathways included glutathione metabolism, purine/pyrimidine metabolism, and histidine metabolism, which are involved in antioxidant defence, nucleotide and protein synthesis for growth and immunity regulation. Furthermore, the gut microbiota composition of B. mori was significantly altered after ZIF8@ZIF67 supplementation, with a marked increase in the relative abundance of Mammaliicoccus and Enterococcus. Lastly, the correlation between metabolites and microbiomes was analysed, including Enterococcus and 1-methylhistidine (r = 0.8895, p = 0.0001), Akkermansia and N-acetyl-d-galactosamine (r = 0.8674, p = 0.0003). These findings demonstrated that ZIF8@ZIF67 could optimise silkworm health by orchestrating metabolite-microbe interactions to enhance nutrient assimilation and oxidative stress resilience, while simultaneously activating pathways essential for growth regulation and immune function.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144283811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Age-associated morphology and transcriptome analyses reveal functional transformation of Malpighian tubules of cicadas in response to development and niche shift. 与年龄相关的形态学和转录组分析揭示了蝉马尔比氏小管在发育和生态位转移过程中的功能转变。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-06-12 DOI: 10.1111/imb.13007

Feimin Yuan, Pengcheng Lian, Lu Liu, Zhen Xie, Zi Li, Tiantian Li, Cong Wei

{"title":"Age-associated morphology and transcriptome analyses reveal functional transformation of Malpighian tubules of cicadas in response to development and niche shift.","authors":"Feimin Yuan, Pengcheng Lian, Lu Liu, Zhen Xie, Zi Li, Tiantian Li, Cong Wei","doi":"10.1111/imb.13007","DOIUrl":"https://doi.org/10.1111/imb.13007","url":null,"abstract":"Malpighian tubules (MTs) of insects are integral to osmoregulation, detoxification, immunity and stress responses. Cicadas experience an ecological transition from subterranean to aboveground during ontogeny. However, little is known about the morphological/functional differences related to MTs between their nymphal and adult stages and potential molecular mechanisms underlying the transition. We described morphological changes of MTs from nymph to adult, including more elongated and densified microvilli and drastically dissolved secretory vacuoles. These alterations indicate MTs functionally transitioned from multifunctional secretory organs in nymphs to primarily excretory organs in adults. Transcriptomic analyses revealed differentially expressed genes were related to osmoregulation, energy metabolism, detoxification, immunity and protein synthesis, etc., across developmental stages. The upregulation of genes involved in ion transport and energy metabolism in adults suggests that enhanced capabilities for rapid waste excretion and energy acquisition are essential for aboveground adults. Increased expression of detoxification and immune-related genes indicates evolutionary adaptations of MTs to manage dietary toxicity and bolster defence mechanisms in adults. These adaptations facilitate the transition from subterranean nymphs to aboveground adults and enhance cicadas' ability to thrive in diverse ecological niches. Our findings infer the role of hormonal regulation in facilitating functional modifications of MTs to adult emergence during cicadas' growth and development. Results of this study have important implications for the evolution of life-history strategy of insects responding to diet/habitat changes during ontogeny. These findings enhance our understanding of how insects adapt to dietary/habitat shift and emphasise the importance of MTs in physiological responses to environmental challenges.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The ci-miR-5 regulates the CiCYP18A1 to control moulting process of Chilo infuscatellus: Prospective target for pest control. ci-miR-5通过调控CiCYP18A1调控红毛螟的蜕皮过程：害虫防治的潜在靶点。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-06-06 DOI: 10.1111/imb.13004

Jin-da Wang, Huan-Tai Lin, Zhi-Han Yang, Li-Fei Chen, Yi-Lu Tong, Ji-Li Wei, Xue-Hong Pan, Xian-Kun Shang, San-Ji Gao

{"title":"The ci-miR-5 regulates the CiCYP18A1 to control moulting process of Chilo infuscatellus: Prospective target for pest control.","authors":"Jin-da Wang, Huan-Tai Lin, Zhi-Han Yang, Li-Fei Chen, Yi-Lu Tong, Ji-Li Wei, Xue-Hong Pan, Xian-Kun Shang, San-Ji Gao","doi":"10.1111/imb.13004","DOIUrl":"https://doi.org/10.1111/imb.13004","url":null,"abstract":"The microRNAs (miRNAs) play an important role in the regulation of gene expression, influencing the outcome of many biological processes in insects. The introduction of miRNA into the insect body can lead to pest developmental abnormalities indicating that miRNAs are potential targets for pest control. In this study, we employed small RNA sequencing to characterise and analyse the expression of miRNAs in the sugarcane shoot borer Chilo infuscatellus (Snellen). By integrating previously published transcriptome sequencing data, we predicted and identified miRNAs and their target genes involved in the ecdysone metabolic pathway. We confirmed the target relationships between Ci-miR-4, Ci-miR-5 and CiCYP18A1 by RT-qPCR and dual-luciferase reporter assays in vivo and in vitro. Additionally, injection of miRNA mimics reduced the transcripts of CiCYP18A1 and led to abnormal moulting, which is similar to the defect phenotype of depletion of CiCYP18A1 by dsRNA. Furthermore, we also mixed the synthesised Ci-miR-5 mimic into artificial diets and found similar stunting results of the injection method. These results demonstrated that Ci-miR-5 could target CiCYP18A1 to regulate the moulting process and the successful use of an insect miRNA for gene silencing studies in this sugarcane pest. The method provided an effective novel approach for future RNAi-based pest control strategies.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the toxic effects and midgut biological changes induced by low concentrations of cyantraniliprole in Bombyx mori. 低浓度氰氨酰胺对家蚕的毒性作用及中肠生物学变化的评价。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-06-05 DOI: 10.1111/imb.13006

Ruinan Qi, Hao Li, Hongrui Jiang, Yue Su, Xiqian Guo, Fanchi Li, Minjin Han, Bing Li, Haina Sun

{"title":"Evaluation of the toxic effects and midgut biological changes induced by low concentrations of cyantraniliprole in Bombyx mori.","authors":"Ruinan Qi, Hao Li, Hongrui Jiang, Yue Su, Xiqian Guo, Fanchi Li, Minjin Han, Bing Li, Haina Sun","doi":"10.1111/imb.13006","DOIUrl":"https://doi.org/10.1111/imb.13006","url":null,"abstract":"Cyantraniliprole (Cya), a diamide insecticide, is widely utilised for the management of Lepidoptera pests owing to its potent insecticidal efficacy and broad spectrum of activity. The extensive use and prolonged environmental persistence of this insecticide pose a significant threat to the sustainable development of sericulture. This study firstly assessed the lethal toxicity of cyantraniliprole to the 5th instar larvae of Bombyx mori. Exposure to cyantraniliprole (LC5, LC10 and LC20) resulted in a concentration-dependent reduction in larval weight, pupal weight and survival rate and a prolongation of larval development time. Moreover, cyantraniliprole LC10 resulted in substantial structural damage to the epithelial cells, suppressed the mRNA levels of oxidative phosphorylation genes, perturbed ATP synthesis and led to an imbalance of intracellular reactive oxygen species. Meanwhile, the starvation treatment suggested that the impacts of cyantraniliprole on silkworms cannot be solely ascribed to nutritional deficiencies. Additionally, the results revealed that cytochrome P450s might serve as a pivotal factor in the detoxification metabolism of cyantraniliprole in the midgut of silkworms. The findings of this study offer evidence for the ecological risk posed by environmental residues of cyantraniliprole to non-target organisms and are also of great significance for sericulture production.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silencing PsASH2 affects embryo development in the cotton mealybug. 沉默PsASH2会影响棉粉蚧的胚胎发育。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-05-30 DOI: 10.1111/imb.13003

Kaixin Wang, Lingqi Zen, Lilu Sheng, Feihuang Lu, Qianjin Lin, Yifan Li, Haojie Tong

引用次数: 0

Differences in gene expression patterns between parthenogenetically and sexually produced offspring during early development of Reticulitermes speratus. 孤雌繁殖和有性繁殖后代早期发育过程中基因表达模式的差异

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-05-30 DOI: 10.1111/imb.13001

Riho Kamiyama, Michihiko Takahashi, Mamoru Takata, Kenji Matsuura

{"title":"Differences in gene expression patterns between parthenogenetically and sexually produced offspring during early development of Reticulitermes speratus.","authors":"Riho Kamiyama, Michihiko Takahashi, Mamoru Takata, Kenji Matsuura","doi":"10.1111/imb.13001","DOIUrl":"https://doi.org/10.1111/imb.13001","url":null,"abstract":"Social insects exhibit reproductive division of labour, governed by both external and internal factors influencing caste determination. In termites with a unique reproductive system known as asexual queen succession (AQS), queens produce neotenic queens via parthenogenesis, while workers and alates arise through sexual reproduction. This inherent caste differentiation bias may have resulted from differences in gene expression potentially influenced by the parent-of-origin effect, as parthenogenetic daughters inherit only maternal genomes, while sexually produced daughters inherit both paternal and maternal genomes. Here, we show that gene expression patterns in developing embryos of the termite Reticulitermes speratus differ significantly between parthenogenetic and sexually produced offspring. However, SNP analysis indicated that these differences were not attributable to the parent-of-origin effect. Through RNA-seq analysis of female embryos post-katatrepsis, we identified 21 genes, including jhbp, nlk, and wge, which are known to be involved in caste differentiation and morphogenesis, with significant expression differences between parthenogenetic and sexually produced daughters. SNP analysis of sexually produced embryos did not reveal any parent-of-origin biased expression except for mitochondrial genes, though 12 genes exhibited colony-specific expression patterns. These findings suggested that early developmental gene expression partly explained caste differentiation biases. Further research is essential to elucidate the molecular mechanisms behind these transgenerational effects, providing insight into the evolution of AQS and complex caste determination in social insects from a gene expression perspective.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histone and N-terminal acetyltransferases play important roles in female reproduction and embryogenesis of the red flour beetle Tribolium castaneum. 组蛋白和n端乙酰转移酶在红粉甲虫雌性生殖和胚胎发生中起重要作用。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-05-29 DOI: 10.1111/imb.13000

Karthi Sengodan, Subba Reddy Palli

{"title":"Histone and N-terminal acetyltransferases play important roles in female reproduction and embryogenesis of the red flour beetle Tribolium castaneum.","authors":"Karthi Sengodan, Subba Reddy Palli","doi":"10.1111/imb.13000","DOIUrl":"https://doi.org/10.1111/imb.13000","url":null,"abstract":"Histone acetyltransferases (HATs) catalyse the addition of acetyl groups to histones and other proteins. In contrast, histone deacetylases remove acetyl groups from core histones, and the activity of these enzymes maintains the acetylation levels of these proteins. Histone acetylation levels influence chromatin accessibility and gene expression and regulate many biological processes, including development and reproduction. Recent reports suggest that some N-terminal acetyltransferases (NATs) also regulate gene expression. We identified 29 HAT and NAT genes in the red flour beetle, Tribolium castaneum, and studied their functions in female reproduction using RNA interference (RNAi). Knockdown of seven out of 13 HAT genes (N-acetyltransferase ESCO2) (ESCO1/2), Elongator complex protein 3 (ELP3), Histone acetyltransferase type B catalytic subunit 1 (HAT1), Transcription initiation factor TFIID subunit 1 (TAF1), Protein x-mas-2 (MCM3AP), Histone acetyltransferase Tip60 (KAT5), and Cysteine-rich protein 2-binding protein (KAT14) and 12 out of 16 NAT genes Probable glucosamine 6-phosphate N-acetyltransferase (GNPNAT1), N-alpha-acetyltransferase 10 (NAA10), N-alpha-acetyltransferase 20 (NAA20), N-alpha-acetyltransferase 30 (NAA30), N-alpha-acetyltransferase 40 (NAA40), N-alpha-acetyltransferase 60 (NAA60), N-acetyltransferase 6 (NAA80), RNA cytidine acetyltransferase (NAT10), Diamine acetyltransferase 2 (SATL1), N(alpha)-acetyltransferase 16 (NAA16), Phagocyte signalling-impaired protein (NAA25), N(alpha)-acetyltransferase 35 (NAA35) caused a significant reduction in eggs laid by females compared to the eggs laid by control females injected with dsGFP. Also, knockdown of nine (KAT5, ATAT1, ELP3, HAT1, KAT8A, NAA10, NAA20, GNPNAT1 and TAF1) HAT/NAT genes caused a significant decrease in egg hatching. Parental RNAi of ATAT1 and KAT8 blocked embryogenesis. These data suggest that the acetylation of proteins plays an important role in female reproduction and embryogenesis.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144173611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temperature-dependent alternative splicing affects gene expression in Aedes aegypti mosquitoes midgut. 温度依赖性选择性剪接影响埃及伊蚊中肠基因表达。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-05-29 DOI: 10.1111/imb.13002

Priscila Gonçalves Ferreira, Miqueias Fernandes, Camila Aparecida Profeta, Renata Cristina Barbosa, Courtney Cuinn Murdock, Gustavo Ferreira Martins, Tiago de Oliveira Mendes

{"title":"Temperature-dependent alternative splicing affects gene expression in Aedes aegypti mosquitoes midgut.","authors":"Priscila Gonçalves Ferreira, Miqueias Fernandes, Camila Aparecida Profeta, Renata Cristina Barbosa, Courtney Cuinn Murdock, Gustavo Ferreira Martins, Tiago de Oliveira Mendes","doi":"10.1111/imb.13002","DOIUrl":"https://doi.org/10.1111/imb.13002","url":null,"abstract":"Temperature is one of the most significant abiotic factors influencing vector resistance to pathogens. Research has provided substantial insights into the immunological and oxidative processes affected by temperature. However, our understanding of the complex interplay in mosquito-pathogen interactions remains limited. In this study, we investigated the impact of temperature on alternative splicing in the midgut of Aedes aegypti mosquitoes (adult females) under different thermal conditions. Few genes exhibited differential alternative splicing when comparing low (20°C) and high (36°C) temperatures to standard rearing conditions (28°C). Among these, Trypsin (TRY), Ferritin (FER), Thioredoxin (TRX) and Peptidoglycan recognition protein LC (PGRP-LC) were identified, aligning with previous findings that their expression is temperature-sensitive. Among the genes identified with alternative splicing, we focus on Thioredoxin and PGRP-LC, genes modulated by temperature variations and promising targets for future studies on their role in the competence of Ae. aegypti to transmit Zika. Experimental validation confirmed that TRX, a gene critical for pathogen defence, has a differentially spliced exon under warmer conditions, potentially altering its activity. In contrast, no differential splicing was observed for PGRP-LC across temperature treatments. These findings suggest that temperature-induced alternative splicing may play a role in shaping the mosquito's physiological responses to environmental changes, highlighting a previously underexplored layer of complexity in mosquito-pathogen dynamics.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144173612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0