Insect Molecular Biology最新文献_第2页

Heat-responsive ame-miR-1-3p modulates thermotolerance in honeybees (Apis mellifera L.). 热反应性名称mir -1-3p调节蜜蜂的耐热性。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-07-25 DOI: 10.1111/imb.70004

Teng-Fei Shi, Zhen-Qing Feng, Zhuang Li, Liang Ye, Xing-Chuan Jiang, Hai-Qun Cao, Lin-Sheng Yu

{"title":"Heat-responsive ame-miR-1-3p modulates thermotolerance in honeybees (Apis mellifera L.).","authors":"Teng-Fei Shi, Zhen-Qing Feng, Zhuang Li, Liang Ye, Xing-Chuan Jiang, Hai-Qun Cao, Lin-Sheng Yu","doi":"10.1111/imb.70004","DOIUrl":"https://doi.org/10.1111/imb.70004","url":null,"abstract":"Thermal stress significantly harms the survival, physiology and development of honeybees (Apis mellifera L.). miRNAs are emerging as key regulators of environmental stress responses. This study investigated honeybee's miRNA expression under heat stress and clarified the role of ame-miR-1-3p in thermotolerance. Global miRNA expression profiles were analysed in forager bees exposed to 45°C for 2 and 4 h using small RNA sequencing (sRNA-seq). To explore the effects of ame-miR-1-3p, young bees were fed miRNA mimics or antagonists to either overexpress or knock down its expression. The impact on target gene expression (Hsp90 and LOC413120) and the bees' heat susceptibility was then assessed. A dual luciferase reporter assay was used to confirm the direct targeting relationship between ame-miR-1-3p and LOC413120. The results showed that exposure to 45°C led to seven and five differentially expressed miRNAs (DEMs) at 2 and 4 h, respectively, all of which were suppressed. Bioinformatic analyses indicated that DEM target genes were enriched in stress-related signalling pathways including MAPK, phosphatidylinositol and Notch. ame-miR-1-3p, the most abundant DEM, was significantly down-regulated under heat exposure, as validated by qRT-PCR. Conversely, the expression of LOC413120 (encoding DnaJ homologue subfamily B member 12), a potential target of ame-miR-1-3p, was upregulated. Increasing ame-miR-1-3p expression significantly suppressed LOC413120 expression and reduced heat resistance, whereas decreasing ame-miR-1-3p expression significantly enhanced LOC413120 expression and increased heat resistance. Furthermore, dual luciferase analysis confirmed that LOC413120 is a direct target of ame-miR-1-3p. These findings reveal a novel function of miRNAs in honeybee heat resistance.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144707390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transformer is involved in female sex determination in Bemisia tabaci. 变压器参与烟草粉虱雌性性别决定。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-07-21 DOI: 10.1111/imb.70000

Yating Liu, Wenlu Wang, Yina Wang, Xuguo Zhou, Wen Xie, Youjun Zhang

{"title":"Transformer is involved in female sex determination in Bemisia tabaci.","authors":"Yating Liu, Wenlu Wang, Yina Wang, Xuguo Zhou, Wen Xie, Youjun Zhang","doi":"10.1111/imb.70000","DOIUrl":"https://doi.org/10.1111/imb.70000","url":null,"abstract":"Sex determination is one of the fundamental questions in developmental and evolutionary biology. While the upstream regulatory mechanisms governing somatic sex determination in insects are highly divergent, the downstream transformer (tra) + transformer2 (tra2) → doublesex (dsx) pathway is relatively conserved. In the sweet potato whitefly, Bemisia tabaci, a global invasive pest, tra2 and dsx have been previously characterised, revealing their critical role in male genitalia formation. However, the role of tra in B. tabaci sex determination has remained unclear. Here, we characterised Bttra, the B. tabaci transformer, and identified multiple TRA/TRA-2 binding sites and several splice variants. A glutathione S-transferase (GST) pull-down assay confirmed direct interactions between BtTRA and BtTRA2 in vitro. Functional analysis through Bttra silencing demonstrated its regulatory impact on Btdsx expression and its essential role in female development. Our combined findings provide new insights into the function of tra in B. tabaci sex determination, highlighting variation in sex determination among haplodiploid insects. Moreover, this study identifies potential molecular targets for the future management of this devastating insect pest.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of transgenic Drosophila suzukii lines that express phiC31 integrase and carry the sepia gene as a marker for transformation. 表达phiC31整合酶并携带棕褐色基因作为转化标记的转基因铃木果蝇系的建立。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-07-17 DOI: 10.1111/imb.70001

Kalindu Ramyasoma Hewawasam, Akihiko Yamamoto, Maxwell J Scott

{"title":"Establishment of transgenic Drosophila suzukii lines that express phiC31 integrase and carry the sepia gene as a marker for transformation.","authors":"Kalindu Ramyasoma Hewawasam, Akihiko Yamamoto, Maxwell J Scott","doi":"10.1111/imb.70001","DOIUrl":"https://doi.org/10.1111/imb.70001","url":null,"abstract":"Many eye colour mutants have been identified in Drosophila melanogaster. Mutations in the sepia gene result in brown eyes due to a lack of PDA synthase, which is essential for production of the red drosopterin eye pigment. We previously used CRISPR/Cas9 to target the PDA synthase gene to establish sepia mutant strains for Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), an invasive global pest of soft skinned fruits. The fecundity and fertility of some of the sepia mutant strains were similar to wild-type. The goal of this study was to determine if the sepia gene could be used as a marker to identify transgenic D. suzukii. By using the sepia gene as a marker, we successfully developed lines expressing Streptomyces phage phiC31 integrase in the germline. For most of these lines, hemizygotes exhibited complete rescue of the sepia eye colour and relatively high levels of phiC31 RNA in ovaries. In contrast, lines with partial rescue showed low levels of sepia RNA in heads and phiC31 RNA in ovaries. These findings suggest that the sepia gene is an effective marker for D. suzukii transgenesis and its relatively small size (1.8 kb) makes it advantageous when assembling large gene constructs. The phiC31 integrase lines established in this study should serve as a valuable resource for future genetic research in D. suzukii, including the further development of strains for genetic biocontrol.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SYNCAS-mediated CRISPR-Cas9 genome editing in the Jewel wasp, Nasonia vitripennis. syncas介导的宝石蜂CRISPR-Cas9基因组编辑。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-07-17 DOI: 10.1111/imb.70002

Filippo Guerra, Sander De Rouck, Eveline C Verhulst

{"title":"SYNCAS-mediated CRISPR-Cas9 genome editing in the Jewel wasp, Nasonia vitripennis.","authors":"Filippo Guerra, Sander De Rouck, Eveline C Verhulst","doi":"10.1111/imb.70002","DOIUrl":"https://doi.org/10.1111/imb.70002","url":null,"abstract":"Genetic engineering is a formidable approach to studying biology. The development of CRISPR-Cas9 has allowed the genetic engineering of insect species from several orders, and in some species, this tool is used routinely for genetic research. However, insect gene editing often relies on the delivery of CRISPR-Cas9 components via embryo injection. This technique has a limitation: some species lay their eggs inside hard substrates or living hosts, making embryo collection impossible or labour intensive. Recently, a variety of techniques that exploit maternal injection of nucleases have been developed to circumvent embryo injection. Yet, despite this variety of maternal delivery techniques, some insects remain refractory to gene editing. One of these is the parasitoid wasp, Nasonia vitripennis, an important hymenopteran model species. In this study, a recently developed method termed SYNCAS was used to perform knock-out (KO) of the cinnabar gene in this wasp, obtaining KO efficiencies up to 10 times higher than reported for other maternal injection approaches. We found up to 2.73% of all offspring to display a KO phenotype, and we obtained up to 68 KO offspring per 100 injected mothers. The optimal timing of injection and provision of hosts for egg laying was determined. With this protocol, routine applications of CRISPR-Cas9 become feasible in this species, allowing reverse genetics studies of genes with unknown associated phenotypes and paving the way for more advanced editing techniques.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The homeobox gene Distal-less is critical for the development of adult body appendages in Bombyx mori. 同源盒基因远端缺失是家蚕成体附属物发育的关键基因。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-07-14 DOI: 10.1111/imb.13015

Guan Man, Peilin Guo, Yutong Liu, Hongxia Kang, Kai Chen, Ye Yu, Anjiang Tan

{"title":"The homeobox gene Distal-less is critical for the development of adult body appendages in Bombyx mori.","authors":"Guan Man, Peilin Guo, Yutong Liu, Hongxia Kang, Kai Chen, Ye Yu, Anjiang Tan","doi":"10.1111/imb.13015","DOIUrl":"https://doi.org/10.1111/imb.13015","url":null,"abstract":"The development of adult body appendages is critical for insects. The homeobox gene Distal-less (Dll) is known for its role in proximodistal patterning, and its role in the development of the appendage has been well studied in the model organism Drosophila melanogaster. However, the biological function of Dll in other insects still remains to be further verified. In this study, we investigated the biological function of Dll in the lepidoptera model insect Bombyx mori. Similar to the expression patterns observed in D. melanogaster, BmDll was highly expressed in the tibia and tarsus of the legs. The BmDll mutants obtained through the binary transgenic CRISPR/Cas9 system appeared to thrive normally and no apparent deleterious phenotypes were observed throughout the embryonic and larval stages. Nevertheless, mutants exhibited severe defects in the development of the appendage, especially at the distal end of legs, from pupal stage to adult stage. RNA-seq analysis revealed that metabolism pathways, extracellular matrix (ECM)-receptor interaction and structural constituent of the cuticle were dysregulated in mutant silkworms. Our study provided genetic evidence that Dll plays an important role in the development of B. mori adult body appendages.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transformer-2 gene is essential for female and male development in Gryllus bimaculatus. 转换-2基因在双斑斑马鱼雌雄发育中起着重要作用。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-07-10 DOI: 10.1111/imb.13016

Li-Dong Shi, Jia-Xin Duan, Ning Zhang, Gang-Qi Fang, Zhu-Qing He, Kai Li

引用次数: 0

RNAi comes of age: Translating insect molecular insights into field applications RNAi时代来临：将昆虫分子见解转化为实地应用

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-07-08 DOI: 10.1111/imb.13012

Umut Toprak

引用次数: 0

CRISPR/Cas9-mediated germline mutagenesis in the subsocial parasitoid wasp, Sclerodermus guani. CRISPR/ cas9介导的瓜硬皮亚社会性寄生蜂种系突变

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-06-28 DOI: 10.1111/imb.13014

Zi Ye, Guanzhen Fan, Yun Wei, Li Li, Feng Liu

{"title":"CRISPR/Cas9-mediated germline mutagenesis in the subsocial parasitoid wasp, Sclerodermus guani.","authors":"Zi Ye, Guanzhen Fan, Yun Wei, Li Li, Feng Liu","doi":"10.1111/imb.13014","DOIUrl":"https://doi.org/10.1111/imb.13014","url":null,"abstract":"The ectoparasitoid wasp Sclerodermus guani (Hymenoptera: Bethylidae), as a subsocial insect, is widely applied in biological control against beetle vectors of pine wood nematodes. Despite significant advances in behavioural research, functional genetics in S. guani remains underdeveloped due to the absence of efficient gene manipulation tools. In this study, we employed CRISPR-mediated mutagenesis to achieve germline gene knockout targeting the eye pigment-associated gene kynurenine 3-monooxygenase (KMO). Phylogenetic analysis revealed that S. guani KMO shares a close relationship with its homologue in Prorops nasuta (Hymenoptera: Bethylidae). Two single-guide RNAs (sgRNAs), coupled with Cas9 protein with and without nuclear localisation signal (NLS) were tested. Both sgRNAs induced specific in vitro DNA cleavage and in vivo heritable indels at the target genomic loci. Homozygous null mutant females and males exhibit a white-eye phenotype, which was identified during pupal stage. Optimal editing efficiency in vivo was achieved using the Cas9-NLS variant. Given the complication of germline gene editing in eusocial Hymenopterans, the application of CRISPR in the subsocial parasitoid wasp S. guani provides an accessible research platform for the molecular evolution of insect sociality.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcriptome of the ant Temnothorax nylanderi is not affected by urbanisation but by rearing conditions. 蚂蚁Temnothorax nylanderi的转录组不受城市化的影响，但受饲养条件的影响。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-06-28 DOI: 10.1111/imb.13011

Nicholas M A Smith, Lauren Jacquier, Elise Gay, Mathieu Molet, Claudie Doums

{"title":"The transcriptome of the ant Temnothorax nylanderi is not affected by urbanisation but by rearing conditions.","authors":"Nicholas M A Smith, Lauren Jacquier, Elise Gay, Mathieu Molet, Claudie Doums","doi":"10.1111/imb.13011","DOIUrl":"https://doi.org/10.1111/imb.13011","url":null,"abstract":"Urbanisation alters the environment of organisms, creating new challenges and opportunities. In ants, urbanisation has been shown to increase resistance to temperature and to pollutants. Here we assessed how urbanisation affects gene expression in workers and queens of the ant Temnothorax nylanderi, a species that inhabits both city and forest habitats. We further examined whether there was a differential expression between two distinct conditions, in the field or after laboratory rearing for 8 months. As expected, the number of differentially expressed genes (DEGs) between workers and queens was large (2830 genes). However, we found that habitat (city or forest) had no clear influence on gene expression in queens and workers of T. nylanderi even when individuals were directly collected from the field. Interestingly, we also found 661 DEGs between field colonies and laboratory-reared ones. The effect of rearing conditions on gene activity was greater in workers than in queens, suggesting that queens are more resilient to environmental changes. The lack of transcriptional differences between habitats is intriguing because of the previously observed differences in life-history traits between city and forest populations.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TcCYP4C1 in combination with trehalose to cope with high-CO2 stress in Tribolium castaneum (Coleoptera) TcCYP4C1与海藻糖联合作用对褐藻高co2胁迫的影响。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2025-06-28 DOI: 10.1111/imb.12997

Xinyu Zhang, Liwen Guan, Yuya Zhang, Fan Zhong, Yuhang Xie, Yi Zhang, Xinyi Zhang, Min Zhou, Can Li, Bin Tang

{"title":"TcCYP4C1 in combination with trehalose to cope with high-CO2 stress in Tribolium castaneum (Coleoptera)","authors":"Xinyu Zhang, Liwen Guan, Yuya Zhang, Fan Zhong, Yuhang Xie, Yi Zhang, Xinyi Zhang, Min Zhou, Can Li, Bin Tang","doi":"10.1111/imb.12997","DOIUrl":"10.1111/imb.12997","url":null,"abstract":"Cytochrome P450 monooxygenase (CYP), an important detoxifying enzyme in insects, is involved in the metabolism and degradation of both exogenous compounds and endogenous substances. To investigate the involvement of the T. castaneum TcCYP4C1 gene in detoxification metabolism under high CO2 and the protective role of trehalose against hypoxic stress in insects. In the present study, after successfully silencing the TcCYP4C1 gene of T. castaneum by double-stranded RNA(dsRNA), the larvae were exposed to 95% CO2. This exposure resulted in a statistically significant increase in larval mortality and a significant elevation in the activity of the carboxylesterase enzyme (CarE). However, a decrease in mortality from 18.15% to 11.24% was observed when larvae were fed trehalose after dsRNA injection. In addition, the gene expression levels of the trehalose metabolism pathway related genes TRE1-3, TRE1-4 and TPS2 were significantly up-regulated after 95% CO2 treatment. In summary, the TcCYP4C1 gene emerges as a pivotal factor in the adaptive response of T. castaneum to high CO2. Trehalose effectively mitigates the detrimental effects resulting from the silencing of TcCYP4C1 and exposure to high CO2 stress in T. castaneum. Our findings not only establish a theoretical foundation for the development of novel pesticides tailored for low-oxygen grain storage environments but also inspire innovative, environmentally sustainable pest management strategies in the grain storage sector.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"34 5","pages":"729-742"},"PeriodicalIF":2.3,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0