Insect Molecular Biology最新文献_第2页

Host trees partially explain the complex bacterial communities of two threatened saproxylic beetles. 寄主树部分解释了两种濒危甲虫复杂的细菌群落。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2024-11-06 DOI: 10.1111/imb.12973

Michał Kolasa, Rama Sarvani Krovi, Radosław Plewa, Tomasz Jaworski, Marcin Kadej, Adrian Smolis, Jerzy M Gutowski, Krzysztof Sućko, Rafał Ruta, Tomasz Olbrycht, Sergey Saluk, Maria Oczkowicz, Łukasz Kajtoch

{"title":"Host trees partially explain the complex bacterial communities of two threatened saproxylic beetles.","authors":"Michał Kolasa, Rama Sarvani Krovi, Radosław Plewa, Tomasz Jaworski, Marcin Kadej, Adrian Smolis, Jerzy M Gutowski, Krzysztof Sućko, Rafał Ruta, Tomasz Olbrycht, Sergey Saluk, Maria Oczkowicz, Łukasz Kajtoch","doi":"10.1111/imb.12973","DOIUrl":"https://doi.org/10.1111/imb.12973","url":null,"abstract":"Microorganisms are integral to ecosystem functioning and host adaptation, yet the understanding of microbiomes in diverse beetle taxa remains limited. We conducted a comprehensive study to investigate the microbial composition of two red flat bark beetle species, Cucujus haematodes and C. cinnaberinus, and assessed the influence of host taxonomic relatedness and host tree species on their microbiomes. We sampled 67 larvae of two Cucujus taxa taken from 11 host tree species. 16S rRNA V4 fragment sequencing revealed distinct microbial communities associated with each Cucujus species, with host tree species significantly influencing microbiome composition. Alpha and beta diversity metrics indicated significant differences between microbial communities in both beetle and host tree species. Principal component analysis indicated distinct clustering based on host tree species but not for beetle species. This overlap could be attributed to the similar ecology of both Cucujus species. The detection of various bacteria, among which some have already been reported in saproxylophagous beetles, suggests that the red flat bark beetles ingest the bacteria via foraging on other wood-dwelling invertebrates. Our findings show the complex interplay between host taxonomy, microhabitat and microbial composition in Cucujus, providing insights into their ecological roles and conservation implications. This research helps to fill the gap in understanding the microbial dynamics of saproxylic beetles, sheds light on factors shaping their microbiomes and highlights the importance of considering both host species and environmental conditions when studying insect-microbe interactions in forest ecosystems.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NPF and sNPF can regulate the feeding behaviour and affect the growth and antioxidant levels of the rice brown planthopper, Nilaparvata lugens. NPF 和 sNPF 可调节稻褐飞虱的取食行为，并影响其生长和抗氧化剂水平。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2024-11-04 DOI: 10.1111/imb.12971

Rui-Chuan Duan, Yu-Ning Zhang, Yan-Hui Wang, Bo-Xuan Xie, Zheng-Ze Du, Fa-Jun Chen

{"title":"NPF and sNPF can regulate the feeding behaviour and affect the growth and antioxidant levels of the rice brown planthopper, Nilaparvata lugens.","authors":"Rui-Chuan Duan, Yu-Ning Zhang, Yan-Hui Wang, Bo-Xuan Xie, Zheng-Ze Du, Fa-Jun Chen","doi":"10.1111/imb.12971","DOIUrl":"https://doi.org/10.1111/imb.12971","url":null,"abstract":"Neuropeptide F (NPF) and short neuropeptide F (sNPF) are important neuropeptides and mainly affect feeding behaviour of insects. However, the regulation of insect feeding behaviour by NPF and sNPF appears to differ between species, and it is not clear how NPF and sNPF regulate the food intake of the brown planthopper (Nilaparvata lugens). Therefore, the functions of NPF and sNPF in regulating food intake and affecting the growth and antioxidant levels of N. lugens fed on host rice plants were investigated by knocking down NPF and sNPF respectively and simultaneously knocking down both of them by RNA interference. The results showed that NPF and sNPF were mainly expressed in the head of N. lugens, and N. lugens increased food intake after NPF and sNPF were knocked down, which was reflected in the prolonged duration of N4a and N4b waves in the electrical penetration graph (EPG) experiment after knocking down NPF and sNPF. In addition, knocking down NPF and sNPF led to the increase of body weight and mortality of N. lugens, and also led to the increase of antioxidant level of N. lugens. So it was concluded that NPF and sNPF could regulate food intake, maintain body weight stability and oxidative balance in N. lugens. Our study clarified the molecular mechanism of NPF and sNPF regulating feeding behaviour and affect the growth and antioxidant level of N. lugens.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of TRPV channels in texture discrimination during Bactrocera dorsalis egg-laying behaviour. TRPV 通道在 Bactrocera dorsalis 产卵行为中的质地辨别中的作用。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2024-10-31 DOI: 10.1111/imb.12970

Zhong-Nian Huang, Feng-Yi Guo, Zi-Yuan Li, Yong-Yue Lu, Yi-Xiang Qi

{"title":"Role of TRPV channels in texture discrimination during Bactrocera dorsalis egg-laying behaviour.","authors":"Zhong-Nian Huang, Feng-Yi Guo, Zi-Yuan Li, Yong-Yue Lu, Yi-Xiang Qi","doi":"10.1111/imb.12970","DOIUrl":"https://doi.org/10.1111/imb.12970","url":null,"abstract":"Deciding where to lay an egg is critical for the survival of insects' offspring. Compared with our understanding of the chemosensory assessment of egg-laying sites, the mechanisms of texture detection are largely unknown. Here, we show that Bactrocera dorsalis, a notoriously agricultural pest laying its eggs within ripening fruits, can discriminate substrate texture during the egg-laying process. Exposure to drugs targeting transient receptor potential vanilloid (TRPV) mechanosensory channels abolished their oviposition preference for hard textures. BdorNan and BdorIav are two members of the TRPV subfamily, and their transcripts were detected in the labellum, the foreleg tarsi and the ovipositor. Then, we successfully obtained knockout strains of each gene using the CRISPR/Cas9 technique. The results showed that BdorNan is required for the discrimination of stiffness difference. BdorIav knockout had no significant effect on the ability of B. dorsalis to choose harder substrates. Our study thus reveals that BdorNan plays a substantial role in the texture assessment of egg-laying behaviour in B. dorsalis.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GC-MS-based metabonomic analysis of silkworm haemolymph reveals four-stage metabolic responses to nucleopolyhedrovirus infection. 基于气相色谱-质谱（GC-MS）的家蚕血淋巴代谢分析揭示了家蚕感染核多角体病毒后的四阶段代谢反应。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2024-10-31 DOI: 10.1111/imb.12972

Zhenyue Su, Yi Li, Zihan Lin, Qing Huang, Xinyu Fan, Zhaoming Dong, Qingyou Xia, Ping Zhao, Xin Wang

{"title":"GC-MS-based metabonomic analysis of silkworm haemolymph reveals four-stage metabolic responses to nucleopolyhedrovirus infection.","authors":"Zhenyue Su, Yi Li, Zihan Lin, Qing Huang, Xinyu Fan, Zhaoming Dong, Qingyou Xia, Ping Zhao, Xin Wang","doi":"10.1111/imb.12972","DOIUrl":"https://doi.org/10.1111/imb.12972","url":null,"abstract":"Silkworm, Bombyx mori, an economically significant insect, plays a crucial role in silk production. However, silkworm breeding is highly susceptible to various pathogens, particularly the Bombyx mori nucleopolyhedrovirus (BmNPV), which poses a serious threat. Recent metabonomic studies have provided insights into the metabolic changes associated with BmNPV infection. BmNPV infection has obvious temporal characteristics. However, few studies have investigated the silkworms infected in different periods. This study employed gas chromatography-mass spectrometry (GC-MS) to perform a comprehensive analysis of haemolymph metabolites in silkworms at 48, 72, 96 and 120 h post-infection (h.p.i.). Through the integration of time-course analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, the study revealed distinct four-stage metabolic characteristics in the silkworm's response to BmNPV infection. At Stage 1 (48 h.p.i.), silkworms activate antioxidant defence mechanisms, with significant enrichment in metabolic pathways involving key antioxidants such as glutathione, to mitigate oxidative stress induced by viral invasion. By Stage 2 (72 h.p.i.), pathways related to amino acid metabolism and protein synthesis become active, indicating an increase in protein synthesis. In Stage 3 (96 h.p.i.), energy metabolism and substance transport pathways are significantly upregulated to support the rapid viral replication and the enhanced locomotor behaviour of silkworm. Finally, at Stage 4 (120 h.p.i.), there is a further enhancement of pathways related to energy metabolism, nucleic acid synthesis, and substance transport, which align with peak viral assembly and release. These findings contribute to an in-depth understanding of the biochemical basis of silkworm resistance to NPV.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockdown of tyrosine hydroxylase gene affects larval survival, pupation and adult eclosion in Plagiodera versicolora. 敲除酪氨酸羟化酶基因会影响 Plagiodera versicolora 的幼虫存活、化蛹和成虫羽化。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2024-10-29 DOI: 10.1111/imb.12967

Xiaolong Liu, Xin Wang, Qi Zhang, Longji Ze, Hainan Zhang, Min Lu

引用次数: 0

Identification and functional study of Fib-L, a major silk fibroin gene component in rice leaf folders. 水稻叶折中主要丝纤维蛋白基因成分 Fib-L 的鉴定和功能研究

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2024-10-28 DOI: 10.1111/imb.12965

Jing Xie, Qiyao Mo, Lina Chen, Zhongyan Zhu, Xiao Liu, Guy Smagghe, Mao Ye, Shangwei Li

{"title":"Identification and functional study of Fib-L, a major silk fibroin gene component in rice leaf folders.","authors":"Jing Xie, Qiyao Mo, Lina Chen, Zhongyan Zhu, Xiao Liu, Guy Smagghe, Mao Ye, Shangwei Li","doi":"10.1111/imb.12965","DOIUrl":"https://doi.org/10.1111/imb.12965","url":null,"abstract":"The rice leaf folder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae), is a major migratory pest in rice agriculture. This pest is characterised by its larvae's ability to fold rice leaves using silk, a behaviour that culminates in the formation of a silken cocoon during the pupal stage. The fibroin light chain (CmFib-L) gene is crucial for silk production, yet its specific function in C. medinalis has reminded elusive. This study presents a comprehensive analysis of the CmFib-L gene, revealing its complete open reading frame (ORF) and expression patterns. Notably, the gene is highly expressed in the fifth-instar larvae and the silk gland, which are critical stages for silk production. Our experiments demonstrate that silencing the CmFib-L gene leads to a reduction in pupal weight, an extension of the pupal stage and a disorganised silk cocoon. Furthermore, the larval behaviour of leaf folding and spinning is significantly impaired when the expression of CmFib-L is downregulated. These findings not only show the importance of fibroin light chain in silk production but also reveal a new target gene to regulate and control the behaviour and development of C. medinalis.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The quest for the best target genes for RNAi-mediated pest control. 寻找 RNAi- 媒介害虫控制的最佳目标基因。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2024-10-25 DOI: 10.1111/imb.12966

Doga Cedden, Gregor Bucher

引用次数: 0

Molecular characterization of a novel thioredoxin-related transmembrane protein gene AcTMX3 that plays important roles in antioxidant defence in Arma chinensis diapause. 新型硫氧还蛋白相关跨膜蛋白基因 AcTMX3 的分子特征，该基因在 Arma chinensis diapause 的抗氧化防御中发挥重要作用。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2024-10-23 DOI: 10.1111/imb.12964

Qiaozhi Luo, Jianjun Mao, Yuyan Li, Mengqing Wang, Lisheng Zhang, Zhongjian Shen

{"title":"Molecular characterization of a novel thioredoxin-related transmembrane protein gene AcTMX3 that plays important roles in antioxidant defence in Arma chinensis diapause.","authors":"Qiaozhi Luo, Jianjun Mao, Yuyan Li, Mengqing Wang, Lisheng Zhang, Zhongjian Shen","doi":"10.1111/imb.12964","DOIUrl":"https://doi.org/10.1111/imb.12964","url":null,"abstract":"Protein disulphide isomerase (PDI) possesses disulphide isomerase, oxidoreductase and molecular chaperone activities, and is involved in regulating various physiological processes. However, there are few studies on the function in insect diapause. In this study, we cloned one novel member PDI family (TMX3, thioredoxin-related transmembrane protein 3) in Arma chinensis. The AcTMX3 encodes 426 amino acids that contains a predicted N-terminal signal sequence, a thioredoxin-like domain with the CXXC active site and a potential transmembrane region, which are typical sequence features of TMX3. RT-qPCR results showed that AcTMX3 was mainly expressed in the head under non-diapause conditions, while AcTMX3 was highly expressed in the fat body (central metabolic organ) under diapause conditions. Moreover, temporal expression profile showed that compared with non-diapause conditions, diapause conditions significantly induced AcTMX3 expression, and the expression of AcTMX3 was enhanced at 15°C. Silencing AcTMX3 in A. chinensis significantly inhibited the expression of antioxidant genes (AcTrx2 and AcTrx-like), increased the content of H2O2 and ascorbate and reduced the survival rate of A. chinensis under diapause conditions. Our results suggested that AcTMX3 played an important role in the resistance of A. chinensis to oxidative stress under diapause conditions.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic gatekeepers: Dynamic roles of sugar transporters in insect metabolism and physiology. 代谢看门人：糖转运体在昆虫新陈代谢和生理学中的动态作用。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2024-10-12 DOI: 10.1111/imb.12963

Bhagyashri Y Chaudhari, Aditya G Pradhan, Rakesh S Joshi

{"title":"Metabolic gatekeepers: Dynamic roles of sugar transporters in insect metabolism and physiology.","authors":"Bhagyashri Y Chaudhari, Aditya G Pradhan, Rakesh S Joshi","doi":"10.1111/imb.12963","DOIUrl":"https://doi.org/10.1111/imb.12963","url":null,"abstract":"Sugars play multiple critical roles in insects, serving as energy sources, carbon skeletons, osmolytes and signalling molecules. The transport of sugars from source to sink via membrane proteins is essential for the uptake, distribution and utilization of sugars across various tissues. Sugar supply and distribution are crucial for insect development, flight, diapause and reproduction. Insect sugar transporters (STs) share significant structural and functional similarities with those in mammals and other higher eukaryotes. However, they exhibit unique characteristics, including differential regulation, substrate selectivity and kinetics. Here, we have discussed structural diversity, evolutionary trends, expression dynamics, mechanisms of action and functional significance of insect STs. The sequence and structural diversity of insect STs, highlighted by the analysis of conserved domains and evolutionary patterns, underpins their functional differentiation and divergence. The review emphasizes the importance of STs in insect metabolism, physiology and stress tolerance. It also discusses how variations in transporter regulation, expression, selectivity and activity contribute to functional differences. Furthermore, we have underlined the potential and necessity of studying these mechanisms and roles to gain a deeper understanding of insect glycobiology. Understanding the regulation and function of sugar transporters is vital for comprehending insect metabolism and physiological potential. This review provides valuable insights into the diverse functionalities of insect STs and their significant roles in metabolism and physiology.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142464288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterisation of the bacteriomes harboured by major wireworm pest species in the Canadian Prairies. 加拿大草原主要线虫害虫物种所含细菌群的特征。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2024-10-09 DOI: 10.1111/imb.12962

Ivan Drahun, Keagan Morrison, Elise A Poole, Willem G van Herk, Bryan J Cassone

{"title":"Characterisation of the bacteriomes harboured by major wireworm pest species in the Canadian Prairies.","authors":"Ivan Drahun, Keagan Morrison, Elise A Poole, Willem G van Herk, Bryan J Cassone","doi":"10.1111/imb.12962","DOIUrl":"https://doi.org/10.1111/imb.12962","url":null,"abstract":"Nearly all insects harbour bacterial communities that can have a profound effect on their life history, including regulating and shaping host metabolism, development, immunity and fitness. The bacteriomes of several coleopterans have been described; however, very little has been reported for wireworms. These long-lived larvae of click beetles (Coleoptera: Elateridae) are major agricultural pests of a variety of crops grown in the Canadian Prairies. Consequently, the goal of this study was to characterise the bacteriomes of five of the most significant pest species within the region: Limonius californicus, Hypnoidus abbreviatus, H. bicolor, Aeolus mellillus and Dalopius spp. To do this, we collected larvae from southern Manitoba fields (pre-seeding) and carried out 16S rRNA sequencing on individual specimens. Our results indicate wireworms have diverse and taxon-rich bacterial communities, with over 400 genera identified predominately from the phyla Proteobacteria, Actinobacteriota, Bacteroidota and Firmicutes. However, each species had nine or fewer genera comprising >80% of their bacteriome. Network analyses revealed some community structuring consistent among species, which may culminate in shaping/regulating host biology. Moreover, the microbial signatures were influenced by both ontogeny (early vs. late stage larvae) and reproductive strategy (sexual vs. parthenogenetic), with a myriad of other factors likely contributing to bacterial diversity that are impossible to resolve from our study. Overall, this metagenomics study represents the first to characterise the bacteriomes of wireworms in the Canadian Prairies and the findings could assist in the development of sustainable management strategies for these important agricultural pests.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0