Insect Molecular Biology最新文献

{"title":"Malaria mosquito antimicrobial defence requires immunity and detoxification gene regulation by Lola","authors":"Heidi Espadas-Álvarez,&nbsp;Verónica Valverde-Garduño","doi":"10.1111/imb.70051","DOIUrl":"10.1111/imb.70051","url":null,"abstract":"<p>Malaria-causing <i>Plasmodium</i> parasites are transmitted by some <i>Anopheles</i> mosquito species. The genetic and immunity basis for vector competence, the mosquitoes' intrinsic ability to transmit pathogens, has been extensively studied in major vectors such as <i>Anopheles gambiae</i> and <i>Anopeles stephensi</i>. In <i>An</i>. <i>gambiae</i>, Lola-type motifs were identified in immunity-related <i>cis</i>-regulatory elements; the Lola transcription factor is known to be involved in <i>Drosophila</i> development. Here, the potential role of <i>lola</i> in mosquito antimicrobial defence was investigated in <i>Anopheles albimanus</i>, a malaria vector in the Americas. The in vivo attenuation of <i>lola</i> was found to compromise mosquito survival to microbial infection. It was also found that the induction of antimicrobial defence genes requires <i>lola</i> upregulation. Genes for antimicrobial peptides, C-lectins, pattern recognition receptors, Clip-domain serine proteases and ML-domain proteins showed <i>lola</i> upregulation dependency. Enzymes involved in the generation and detoxification of reactive oxygen species, as well as components of the JNK immune signalling pathways, are also dependent on <i>lola</i> upregulation. These findings suggest that <i>lola</i> regulates the expression of immunity and detoxification genes and that there is a functional link between JNK signalling and <i>lola</i> expression in the mosquito midgut. Dysregulation of this pathway may contribute to increased pathogen invasion and malaria transmission.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 5","pages":"576-593"},"PeriodicalIF":3.3,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imb.70051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148294957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional responses of Aedes aegypti chemosensory tissues in response to volatile DEET and citronella oil","authors":"Ivan Drahun,&nbsp;Jessica M. Sparrow,&nbsp;Cody W. Koloski,&nbsp;Bryan J. Cassone","doi":"10.1111/imb.70049","DOIUrl":"10.1111/imb.70049","url":null,"abstract":"<p>DEET and citronella oil are two of the most widely used mosquito repellents; however, their impact on chemosensory tissues is not fully understood. In their vapour phase, the repellency effect involves the olfactory system, with mosquitoes detecting and responding to diverse odorants through their antennae and mouthparts (maxillary palps and proboscis). In this study, we first performed repellency behavioural assays that indicated both DEET and citronella effectively impaired female <i>Aedes aegypti</i> host-seeking capabilities (i.e. landing on a host and taking a blood meal). We next used RNA sequencing to assess global gene expression changes in <i>Ae. aegypti</i> antennae, mouthparts and headless bodies in response to volatile repellent exposure. The resultant transcriptional changes were considerably greater in the mosquito mouthparts than in the other tissues. The most notable responses in olfactory-related genes to both repellents were the suppressed expression of the three subunits comprising the mosquito CO₂ receptor (Gr1, 2 and 3). Genes associated with mitochondrial function and metabolism were also suppressed. Exposure to DEET was further characterized by substantial inhibition of cytochrome P450 mRNA expression, the primary detoxification enzymes in insects. Overall, our study provides novel insights into the olfactory and physiological responses of <i>Ae. aegypti</i> chemosensory tissues in response to DEET and citronella exposure.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 5","pages":"526-540"},"PeriodicalIF":3.3,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imb.70049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148163053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic and proteomic signatures underlying nymphal adaptation and foam production in the forage pest Mahanarva spectabilis","authors":"Monique da Silva Bonjour,&nbsp;Angelo José Rinaldi,&nbsp;Eulálio Gutemberg Dias dos Santos,&nbsp;Gabriely Teixeira Bhering Faria,&nbsp;Ana Márcia Escocard de Azevedo Manhães,&nbsp;Jorge Fernando Pereira,&nbsp;Alexander Machado Auad,&nbsp;Maria Goreti de Almeida Oliveira,&nbsp;Humberto Josué de Oliveira Ramos","doi":"10.1111/imb.70050","DOIUrl":"10.1111/imb.70050","url":null,"abstract":"<p>The spittlebug <i>Mahanarva spectabilis</i> (Distant, 1909) (Hemiptera: Cercopidae) is an important pest of forage grasses in South America, where its nymphs cause pasture damage by feeding on xylem sap and producing a characteristic foam that protects them against environmental stressors. To investigate the molecular basis of this adaptation, we integrated RNA-seq analysis of nymphs with LC–MS/MS proteomics of the Batelli gland, the primary source of foam secretion. De novo assembly of 100,666 unigenes revealed broad functional diversity, with strong representation of detoxification enzymes (CYP450s, GSTs, UGTs, carboxylesterases), transporters and ion pumps, cuticle proteins, and stress- and immunity-related genes. Nearly 16% of loci exhibited alternative splicing, particularly within detoxification, chemosensory and osmoregulatory gene families, highlighting evidence of transcriptomic variability. Signal peptide and secreted protein predictions identified 168 high-confidence candidate secreted proteins, including detoxification enzymes, proteases, structural proteins and immune-related factors, several of which are consistent with antimicrobial and surfactant-related functions. Proteomic profiling of the Batelli gland confirmed 500 proteins, enriched in chaperones, metabolic enzymes, detoxification pathways and osmoregulatory components, with the most abundant proteins corresponding to Hsp70 chaperones, ATP synthases, cuticle proteins and carbonic anhydrases. Together, these results provide an integrative transcriptomic and proteomic overview for <i>M. spectabilis</i> nymphs, highlighting genes and proteins associated with xylem feeding, foam production and responses potentially related to environmental stress tolerance. This comprehensive dataset not only advances the understanding of spittlebug biology but also identifies candidate molecular targets that may inform innovative strategies for controlling nymphal stages and mitigating spittlebug damage in forage systems.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 5","pages":"556-575"},"PeriodicalIF":3.3,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imb.70050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148239236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host-independent metagenomics reveal gut bacteria contribution to Delia antiqua growth by vitamin B6 provision","authors":"Luyao Lin,&nbsp;Guiyue Gao,&nbsp;Shuoshuo Sun,&nbsp;Xiaoqing Wu,&nbsp;Susu Fan,&nbsp;Hengliang Wang,&nbsp;Fangyuan Zhou,&nbsp;Xinjian Zhang","doi":"10.1111/imb.70046","DOIUrl":"10.1111/imb.70046","url":null,"abstract":"<p>Insect guts host a diverse and abundant array of microorganisms. These microbes improve host fitness by extensively involving in a range of crucial physiological processes, which have mainly been revealed by high-throughput sequencing, particularly metagenomics. However, it is almost impossible to make an accurate and complete distinction between the genetic functions of microbial symbionts and insect hosts without host genome data. By comparing metagenomic data from gut germ-free and nonaxenic larvae, we accurately identified the data belonging to the gut microbiome of the onion maggot <i>Delia antiqua</i> (Diptera: Anthomyiidae). Besides, a correlation between bacteria of the genus <i>Wohlfahrtiimonas</i> (Gammaproteobacteria: Pseudomonadaceae) and vitamin B6 metabolism was detected through collinearity analysis. Furthermore, in vitro tests confirmed that the gut bacterium <i>Wohlfahrtiimonas larvae</i> contributed to the growth of <i>D. antiqua</i> larvae via the independent synthesis of vitamin B6. This study provides a comprehensive view of the gut bacterial diversity in <i>D. antiqua</i> and reveals a functional profile that is strictly specific to the gut microbiota of this species. It has preliminarily revealed the functional differentiation between insect hosts and their symbiotic microorganisms. This study also offers a technical reference for the study of microbial symbiotic functions in other insect-microbe symbioses without host genomic data.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 5","pages":"489-503"},"PeriodicalIF":3.3,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148029861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of olfactory receptor and odorant-binding protein gene families and their roles in Heliothine chemosensory evolution","authors":"Rong Guo,&nbsp;Boyang Ni,&nbsp;Megan L. Fritz","doi":"10.1111/imb.70052","DOIUrl":"10.1111/imb.70052","url":null,"abstract":"<p>Chemosensory systems play key roles in the survival and reproductive success of insects. Two large and diverse chemosensory gene families, odorant receptors (ORs) and odorant-binding proteins (OBPs), play critical roles in insect chemosensation and mediate odour-guided behaviours. In the process of insect chemosensation, odorants from the environment pass through pores in the antennal sensilla and become soluble in the sensillar lymph, either directly on contact or by binding to an OBP. Solubilized odour molecules diffuse through the lymph until they reach and activate their cognate ORs, sending electrophysiological signals to the insect brain. To better understand the evolutionary roles of OR and OBP gene families among members of the Heliothinae, we systematically characterized these two gene families in <i>Chloridea virescens</i> (Lepidoptera: Noctuidae). A total of 81 ORs and 49 OBPs were identified genome-wide. Based on the number and positions of conserved cysteine residues, the OBPs were classified into three types: 34 Classic OBPs, 8 Minus-C OBPs and 7 Plus-C OBPs. Phylogenetic analyses identified potential gene duplications and losses within OR and OBP gene families among members of the Heliothinae, which may be associated with differences in their volatile sensation and olfactory behaviours. Further motif and structural analyses identified a conserved region that was unique among pheromone receptors and predicted as key residues of the binding pocket, implying its critical role in pheromone detection. Future work should focus on experimentally validating its function. Overall, our findings provide important insights into how chemosensory gene evolution contributes to ecological adaptation and reproductive isolation in the Heliothine moths.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 5","pages":"594-612"},"PeriodicalIF":3.3,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imb.70052","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148345058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative transcriptomic and machine learning approaches reveal candidate genes for silk and venom production in Bibionomorpha (Diptera).","authors":"Daniel Kenji Matuo, João Alfredo Teodoro, Danilo T Amaral","doi":"10.1111/imb.70061","DOIUrl":"https://doi.org/10.1111/imb.70061","url":null,"abstract":"<p><p>Insects display adaptive diversity in their secretory systems, which mediate ecological interactions ranging from defence to adhesion. Within Diptera, the co-occurrence of silk and venom represents a rare functional combination. Here, we integrate multi-species transcriptomics, coexpression network analysis and interpretable deep learning to investigate the physiological and evolutionary architecture underlying these traits. Using de novo assemblies from 11 Bibionomorpha species, we identified thousands of orthogroups and modelled their expression across species with Weighted Gene Coexpression Network Analysis and categorical neural classifiers. The models achieved near-perfect discrimination of venom and silk phenotypes (AUC >0.95), enabling functional inference at the orthogroup level. Venom-associated genes were enriched in proteolytic and inhibitory domains (trypsin-like, WAP, Kunitz, serpin), while silk-related genes exhibited chitin-binding CBM14/peritrophin motifs typical of adhesive matrices, supporting their physiological specialisation. AlphaFold3 structural predictions confirmed these domain-level signatures and revealed conserved disulfide-stabilized folds, supporting extracellular secretion. The minimal overlap between silk- and venom-associated modules indicates independent evolutionary origins of these secretory systems, exemplifying adaptive molecular convergence. Our findings provide molecular, structural and physiological insights into the adaptive evolution of secretory systems in Diptera, offering new perspectives for understanding the evolution of insect physiological innovations.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148792615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of neuronal protein NOS in regulation of magnetic orientation in the subterranean termite Odontotermes formosanus.","authors":"Wenjing Wang, Yongyong Gao, Qi Long, Zhengxiong Jiang, Qiuying Huang","doi":"10.1111/imb.70060","DOIUrl":"https://doi.org/10.1111/imb.70060","url":null,"abstract":"<p><p>Magnetic orientation is essential for subterranean termites during foraging and homing. Although neuronal mechanisms are known to be central to magnetoreception, the specific regulatory proteins involved in this process remain unclear. Here, we report that the expression of the neuronal protein nitric oxide synthase (NOS) in the subterranean termite Odontotermes formosanus was significantly reduced under a strong magnetic field (0.1 mT) and upon elimination of the horizontal component of the geomagnetic field. RNA interference-mediated silencing of NOS led to significant downregulation of the magnetoreception gene Cryptochrome 2 (Cry2) and disrupted magnetic orientation in O. formosanus, indicating that NOS is integral to magnetoreception. Consistently, pharmacological inhibition of NOS by L-NAME decreased nitric oxide (NO) levels and abolished magnetic orientation. Together, our behavioural and molecular evidences suggest that NOS plays a critical regulatory role in termite magnetic orientation, which provides new insights into the molecular mechanisms underlying insect magnetoreception.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148630379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bmlebocin3 could inhibit Bombyx mori cytoplasmic polyhedrosis virus in silkworms via the immune deficiency pathway and through the 20-hydroxyecdysone modulations.","authors":"Mian Muhammad Awais, Nasir Mehmood, Wensheng Yu, Jingchen Sun, Haixu Bian, Huichao Yan","doi":"10.1111/imb.70059","DOIUrl":"https://doi.org/10.1111/imb.70059","url":null,"abstract":"<p><p>Antimicrobial peptides (AMPs) are critical components of the innate immune system in insects, but their regulation and contribution to antiviral defence remain poorly understood. In this study, we investigated the expression, antiviral activity and regulation of Bombyx mori Lebocin-3 (BmLEB3) during infection with Bombyx mori cytoplasmic polyhedrosis virus (BmCPV). BmLEB3 expression was markedly induced following BmCPV infection in the silkworms. RNAi-mediated knockdown of BmLEB3 increased the transcriptional level of viral marker gene vp1 in BmN cells, whereas overexpression led to the inhibition of viral replication in BmN cells. Mechanistically, BmCPV induced the expression of the pattern-recognition receptor BmPGRP-S1 and the transcription factor BmRelish, and silencing of either gene reduced BmLEB3 expression, suggesting that immune deficiency (IMD)-associated signalling contributes to BmLEB3 regulation. Moreover, BmLEB3 was induced by 20-hydroxyecdysone (20E) treatment, further strengthening its antiviral effect. Molecular docking analysis revealed a potential interaction between 20E and BmPGRP-S1, indicating a possible hormonal modulation of IMD-associated AMP expression. Collectively, our results identify BmLEB3 as a virus-responsive AMP and suggest that its expression is modulated by IMD-associated signalling and hormonal cues during BmCPV infection in B. mori. These findings provide insight into the molecular regulation of antiviral immune responses in the silkworm and provide a basis for improving disease resistance in sericulture against microbial pathogens.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148630343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA activation in insects: From emerging evidence to molecular applications.","authors":"Sassan Asgari","doi":"10.1111/imb.70058","DOIUrl":"https://doi.org/10.1111/imb.70058","url":null,"abstract":"<p><p>RNA interference is commonly used as a reverse genetics tool to investigate gene function. In insects, this is achieved by introducing double-stranded RNA or synthetic short-interfering RNAs (siRNAs). However, RNA activation (RNAa) is less frequently utilised in these studies. RNAa can be achieved by targeting a gene's promoter with antisense RNA, which triggers endogenous transcription. Unlike the siRNA mechanism, which is well characterised, the RNAa mechanism is less well known. In the literature, 'RNAa' has been adopted to describe diverse small RNA-mediated upregulation mechanisms that may not necessarily function through the canonical RNAa mechanism described in mammals, which involves interaction of the small RNA with the promoter region. Although the outcomes of RNAi and RNAa differ, some components are shared between their pathways, such as Argonaute proteins. In this minireview, RNAa, its canonical and non-canonical mechanisms, and applications in insect research are examined with the aim of encouraging its use as a tool in Insect Molecular Biology.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148420911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of catalpol on glycolipid homeostasis and lifespan of Drosophila melanogaster","authors":"Yan Li,&nbsp;Yuxin Ge,&nbsp;Yanlin Luo,&nbsp;Sunhong Zhu,&nbsp;Min Zhou,&nbsp;Yongsheng Chang,&nbsp;Bin Tang","doi":"10.1111/imb.70038","DOIUrl":"https://doi.org/10.1111/imb.70038","url":null,"abstract":"<p>Glycolipid metabolism is a core regulatory link in the energy homeostasis and physiological functions of insects, directly affecting their survival, development, reproduction and environmental adaptability. Catalpol, the main active component of <i>Rehmannia glutinosa</i>, possesses multiple pharmacological activities, such as antioxidation, anti-inflammation and regulation of glycolipid metabolism. However, the specific regulatory mechanism of its effect on insect metabolism remains unclear. In this study, <i>Drosophila melanogaster</i> was used as the model organism to systematically explore the effects of catalpol on glycolipid metabolism, growth and development, motor ability and lifespan of female adults, as well as its underlying molecular mechanism. At the molecular level, catalpol significantly enhances glucose catabolism, as demonstrated by increased activity of both soluble and membrane-bound trehalase and upregulation of the <i>Tret1-1</i> gene. Concurrently, it dynamically regulated lipid metabolism, significantly increasing the content of free fatty acids and triglycerides. This finding, coupled with the upregulation of key lipolytic genes such as <i>hormone-sensitive lipase</i> (<i>HSL</i>), indicates accelerated fat turnover. These metabolic modulations translated into significant phenotypic improvements. Catalpol treatment promoted insect growth and development, as evidenced by increased body weight, abdominal size and wing area. Furthermore, it enhanced their motor ability, shown by improved climbing, and extended their average lifespan and survival rate, suggesting a delay in ageing. In conclusion, this study elucidates a novel regulatory model, wherein catalpol dynamically modulates both glucose and lipid metabolism, leading to improved growth, enhanced motor function and extended lifespan in <i>D. melanogaster</i>. Our findings provide initial insights into the intricate mechanism-phenotype connection and provide compelling experimental evidence for catalpol's potential as an insect physiological regulator. This research not only offers new perspectives on insect energy homeostasis but also lays a foundation for its application in agricultural pest control and beneficial insect breeding.</p>","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 4","pages":"386-398"},"PeriodicalIF":3.3,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148522931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}