Insect Molecular Biology最新文献_第2页

Knockout of Ku70 and Ku80 elevates homology-directed repair efficiency in Plutella xylostella. 敲除Ku70和Ku80可提高小菜蛾同源定向修复效率。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2026-04-14 DOI: 10.1111/imb.70039

Faisal Munir, Uroosa Zaheer, Muhammad Asad, Guang Yang

{"title":"Knockout of Ku70 and Ku80 elevates homology-directed repair efficiency in Plutella xylostella.","authors":"Faisal Munir, Uroosa Zaheer, Muhammad Asad, Guang Yang","doi":"10.1111/imb.70039","DOIUrl":"https://doi.org/10.1111/imb.70039","url":null,"abstract":"The suppression of Ku70 and Ku80 has been verified to increase homology-directed repair (HDR) efficiency in fruit fly, silkworm and mosquito, but not in other insects. In this study, PxKu70 and PxKu80 were identified from the Plutella xylostella genome. Domain analysis revealed that PxKu70 contained three conserved domains: Ku N, Ku and Ku C, while PxKu80 comprised the Ku N, Ku and Ku PK bind domains. Phylogenetic analysis and multiple-sequence alignment indicated strong conservation of both proteins among lepidopteran insects. RT-qPCR analysis showed that PxKu70 and PxKu80 were highly expressed in adult stages, particularly in reproductive tissues such as the ovary and testis, suggesting their role in maintaining genomic stability during gametogenesis. Two homozygous knockout lines (ΔPxKu70 and ΔPxKu80) were successfully generated through CRISPR/Cas9-mediated genome editing. These knockout lines remained viable and fertile without observable fitness effects. A donor construct carrying an EGFP cassette designed for insertion at the PxKmo locus was generated to assess HDR-mediated integration. The HDR insertion rate was significantly elevated in both knockout lines compared with the wild-type. These findings demonstrate that suppression of either PxKu70 or PxKu80 can enhance HDR in P. xylostella, offering an effective approach for precise genome editing in lepidopteran species.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147689819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging experimental and bioinformatic approaches in RNA interference-based pest control research. 基于RNA干扰的害虫防治研究中新兴的实验和生物信息学方法。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2026-04-07 DOI: 10.1111/imb.70040

Doga Cedden

{"title":"Emerging experimental and bioinformatic approaches in RNA interference-based pest control research.","authors":"Doga Cedden","doi":"10.1111/imb.70040","DOIUrl":"https://doi.org/10.1111/imb.70040","url":null,"abstract":"RNA interference (RNAi) has emerged as a promising strategy for species-specific and environmentally friendly pest control, offering an alternative to conventional chemical insecticides that are increasingly constrained by resistance development and ecological concerns. RNAi-based approaches involve oral delivery of double-stranded RNA (dsRNA), which is processed into RNA-induced silencing complex (RISC)-bound small interfering RNA (siRNA) to silence essential genes of pests. This review synthesizes recent advances in experimental and bioinformatic methodologies that are facilitating and enhancing RNAi research in insect pest management. Particular emphasis is placed on molecular validation techniques that move beyond phenotype-based bioassays, including RISC-bound small RNA sequencing to resolve dsRNA processing and guide strand selection, RNA degradomics to map siRNA-mediated transcript cleavage events and transcriptomic and proteomic profiling to characterize genome-wide responses and compensatory effects. In parallel, dsRNA visualization methods provide mechanistic insight into uptake, intracellular trafficking and degradation dynamics, clarifying barriers that distinguish responsive from recalcitrant species. Complementing these experimental developments, emerging computational platforms enable insect-optimized target selection, dsRNA design and environmentally informed off-target prediction. Together, these innovations support a transition toward more predictive and mechanistically grounded RNAi-based pest control applications. The integration of high-resolution molecular tools with specialized bioinformatic pipelines is expected to enhance efficacy, safety and reproducibility, advancing RNAi-based pest control toward practical and scalable agricultural deployment.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147627725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-133 activates notch signalling by repressing Synaptobrevin to control ligand localisation in Drosophila. miR-133通过抑制Synaptobrevin来激活notch信号，从而控制果蝇的配体定位。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2026-03-26 DOI: 10.1111/imb.70036

Cheng-Hsien Lee, Yun-Chien Hung, Jian-Chiuan Li, Chun-Hong Chen, Yu-Chen Tsai

{"title":"miR-133 activates notch signalling by repressing Synaptobrevin to control ligand localisation in Drosophila.","authors":"Cheng-Hsien Lee, Yun-Chien Hung, Jian-Chiuan Li, Chun-Hong Chen, Yu-Chen Tsai","doi":"10.1111/imb.70036","DOIUrl":"https://doi.org/10.1111/imb.70036","url":null,"abstract":"Precise regulation of Notch signalling is essential for proper eye development in Drosophila. Although the core components of Notch signalling are well characterised, the mechanisms modulating ligand activity remain less understood. Here, we identified microRNA miR-133 as a positive regulator of Notch signalling in a Drosophila eye model. In a Notch-sensitised background, miR-133 overexpression rescued the small-eye phenotype induced by fringe (fng), while miR-133 knockdown exacerbated this defect. miR-133 directly targets Synaptobrevin (Syb), a vesicle-associated SNARE protein, through a conserved site in its 3'UTR. Loss of Syb phenocopied miR-133 overexpression, whereas Syb overexpression enhanced Notch-related defects. miR-133 and Syb exhibited complementary expression patterns, and both miR-133 overexpression and Syb knockdown induced non-autonomous activation of Notch targets and accumulation of Delta and Serrate ligands at the plasma membrane. Furthermore, we show that the E3 ligase Godzilla (Gzl) and Rab11-dependent recycling are essential for Notch activation. Together, our findings revealed a miR-133/Syb/gzl/Rab11 regulatory axis that enhances Notch signalling by modulating ligand trafficking and distribution during development.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147520961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fungal, not insect: Revisiting a misidentified Bemisia tabaci protein interacting with a begomovirus coat protein. 真菌，而不是昆虫：重新审视与begomvirus外壳蛋白相互作用的错误鉴定的烟粉虱蛋白。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2026-03-16 DOI: 10.1111/imb.70037

Jesús Navas-Castillo, Elvira Fiallo-Olivé

{"title":"Fungal, not insect: Revisiting a misidentified Bemisia tabaci protein interacting with a begomovirus coat protein.","authors":"Jesús Navas-Castillo, Elvira Fiallo-Olivé","doi":"10.1111/imb.70037","DOIUrl":"https://doi.org/10.1111/imb.70037","url":null,"abstract":"Understanding interactions between viral proteins and those of their biological vectors is essential for elucidating transmission mechanisms and identifying potential targets for disease control. Insect vectors, among the most common and significant transmitters of animal and plant viruses, play a crucial role in viral transmission dynamics. Such interactions can influence virus transmission efficiency and provide insights into how viral diseases spread within ecosystems. Accurate identification of the insect proteins involved is vital, as misidentifications can lead to erroneous conclusions that misguide subsequent research and control strategies. In the case of begomoviruses (Geminiviridae: Begomovirus), a protein previously identified as insect-derived by Ohnesorge and Bejarano (2009) is shown here, based on the analyses presented in this Commentary, to be of likely fungal origin. This misidentification, based on yeast two-hybrid and pull-down experiments, has been repeatedly cited in the literature, perpetuating an incorrect conclusion. Establishing the correct identity of this protein is essential for guiding future research on insect-virus interactions.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147468017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and functional characterization of key ecdysteroid biosynthesis and signalling genes regulating moulting and metamorphosis in Dastarcus helophoroides. 牡丹脱毛和变态关键激素生物合成及信号基因的鉴定与功能研究。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2026-03-10 DOI: 10.1111/imb.70035

Tayyab Shaheen, Yan Sun, Jiali Guo, Mengxue Zhang, Zhaochen Wu, Zhengqing Zhang, Guanghui Tang

{"title":"Identification and functional characterization of key ecdysteroid biosynthesis and signalling genes regulating moulting and metamorphosis in Dastarcus helophoroides.","authors":"Tayyab Shaheen, Yan Sun, Jiali Guo, Mengxue Zhang, Zhaochen Wu, Zhengqing Zhang, Guanghui Tang","doi":"10.1111/imb.70035","DOIUrl":"https://doi.org/10.1111/imb.70035","url":null,"abstract":"Dastarcus helophoroides is an important ectoparasitoid used in the biological control of longhorn beetles. Its first-instar larvae actively seek hosts and progress through four larval instars before pupation. Throughout these developmental stages, 20-hydroxyecdysone (20E) plays a pivotal role in initiating and regulating moulting and metamorphosis; however, the specific genes and underlying molecular mechanisms in D. helophoroides remain uncharacterized. In this study, comparative transcriptome analysis across larval development identified differentially expressed genes (DEGs) associated with cuticle remodelling, hormone metabolism and stage-specific metabolic and cellular reprogramming. RT-qPCR validation revealed stage-specific expression patterns of 20E biosynthesis and signalling pathway genes, with EcR, Br-C, E93 and HR39 peaking during the larval-prepupal transition, whereas CYP18A1, E74A, E75B, HR3 and FTZ-F1 were highly expressed in early instars. RNAi targeting the eight key genes (CYP315A1, CYP314A1, EcR, USP, Br-C, E93, HR39 and FTZ-F1) severely disrupted metamorphosis and reduced adult eclosion by 47.83-100%. The developmental arrests were accompanied by stage-specific morphological abnormalities, including abnormal pigmentation, wing deformities and malformed prepupae and pupae. This study identifies key ecdysteroid biosynthesis and signalling genes as critical regulators of D. helophoroides development, providing insights into growth regulation and artificial rearing optimization in this parasitoid biocontrol agent.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147432727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

2-Oxocarboxylic acid metabolic pathway fuels mitochondrial ATP synthesis to power oviposition in Bactrocera dorsalis. 2-氧羧酸代谢途径为背小实蝇线粒体ATP合成提供燃料，为产卵提供动力。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2026-03-07 DOI: 10.1111/imb.70033

Long Ye, Qiaoling Zhang, Jingwei Yang, Daifeng Cheng

{"title":"2-Oxocarboxylic acid metabolic pathway fuels mitochondrial ATP synthesis to power oviposition in Bactrocera dorsalis.","authors":"Long Ye, Qiaoling Zhang, Jingwei Yang, Daifeng Cheng","doi":"10.1111/imb.70033","DOIUrl":"https://doi.org/10.1111/imb.70033","url":null,"abstract":"For many insects, oviposition is an energy-intensive behaviour. However, the metabolic mechanisms powering this process are poorly understood for many species. Here, we investigated the link between energy metabolism and oviposition in Bactrocera dorsalis. The results showed that maturation of the ovipositor was accompanied by a significant increase in mitochondrial number. Transcriptomic analysis identified the 2-oxocarboxylic acid metabolic pathway as the most significantly up-regulated during development. Six core genes (citrate synthase [CS], aconitase [ACO], dehydrogenase 3 [IDH3]α, IDH3β, IDH3γ, glutamic-oxaloacetic transaminase 1 [GOT1]) within this pathway showed continuous expression increases, peaking at the oviposition stage, and were specifically enriched in ovaries and mature ovipositors. RNAi-mediated co-silencing of IDH3 subunits or individual knockdown of CS, ACO or GOT1 significantly reduced both egg deposition and ovipositor ATP levels, whereas individual IDH3 subunit knockdowns showed no significant effect. The ATP synthase inhibitor oligomycin A similarly induced a significant reduction in fecundity and disrupted ovipositor ATP homeostasis. Our study demonstrates that B. dorsalis oviposition is critically dependent on a tissue-specific 2-oxocarboxylic acid metabolic pathway that fuels mitochondrial ATP synthesis in the ovipositor. This establishes a direct functional link between core energy metabolism and reproductive behaviour, revealing novel potential targets for pest control.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147372497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of receptor tyrosine kinase family member CAD96CA on hormone signalling and ontogeny of silkworm 受体酪氨酸激酶家族成员CAD96CA对家蚕激素信号传导和个体发育的影响。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2026-03-03 Epub Date: 2025-11-21 DOI: 10.1111/imb.70018

Chaojie Chen, Hao Sun, Tao Zhong, Dan Liu, Junjie Lao, Yan Zhang, Zijian Shi, Jingya Chen, Mengting Shen, Sanyuan Ma, Ling Jia

{"title":"Effect of receptor tyrosine kinase family member CAD96CA on hormone signalling and ontogeny of silkworm","authors":"Chaojie Chen, Hao Sun, Tao Zhong, Dan Liu, Junjie Lao, Yan Zhang, Zijian Shi, Jingya Chen, Mengting Shen, Sanyuan Ma, Ling Jia","doi":"10.1111/imb.70018","DOIUrl":"10.1111/imb.70018","url":null,"abstract":"Moulting and metamorphosis are fundamental physiological processes in the growth and development of holometabolous insects, primarily regulated by ecdysteroids and juvenile hormone (JH), which are synthesized and secreted by the prothoracic gland and corpora allata, respectively. However, the signalling regulatory network involved in the synthesis of these hormones and their effects is complex and interactive and involves many unidentified functional genes. In this study, we conducted a basic bioinformatics analysis of the CAD96CA gene and obtained CAD96CA mutants at the individual level in domestic silkworms using the CRISPR-Cas9 technology. We analysed the growth, development and silk protein synthesis phenotypes of the mutants and detected the synthesis and signalling effects of 20E and JH. The results revealed that knocking out the CAD96CA gene resulted in inhibited larval growth, reduced silk protein production, hindered larval-pupal transition and led to larval mortality. The synthesis of 20E and its signalling pathways, as well as the signalling pathways of JH, were all affected to varying degrees following CAD96CA knockout. Our study elucidates the role of CAD96CA in the growth and development of silkworms and provides a reference for studying metamorphosis mechanisms.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 2","pages":"197-208"},"PeriodicalIF":2.3,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imb.70018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145573676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbohydrate biosynthesis pathway is associated with lineage-specific diapause termination in the silkworm, Bombyx mori 家蚕碳水化合物生物合成途径与谱系特异性滞育终止有关。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2026-03-03 Epub Date: 2025-11-24 DOI: 10.1111/imb.70015

Dongbin Chen, Dehong Yang, Yongping Huang, Zulian Liu

{"title":"Carbohydrate biosynthesis pathway is associated with lineage-specific diapause termination in the silkworm, Bombyx mori","authors":"Dongbin Chen, Dehong Yang, Yongping Huang, Zulian Liu","doi":"10.1111/imb.70015","DOIUrl":"10.1111/imb.70015","url":null,"abstract":"Diapause is a crucial biological adaptation that enables many insects to endure unfavourable environmental conditions and maintain species survival. The silkworm, Bombyx mori, a classic model organism exhibiting embryonic diapause, provides an excellent system for investigating diapause mechanisms. In this study, we evaluated the effectiveness of electrical stimulation in terminating diapause and explored its underlying regulatory mechanisms during the termination process in silkworms. Specifically, electrical stimulation significantly improved the economic traits of silkworms compared to HCl treatment. Transcriptome sequencing was performed to analyse dynamic gene regulation during diapause termination following electrical stimulation in two silkworm strains: the Chinese lineage strain Qiufeng and the Japanese lineage strain Baiyu. Gene ontology (GO) enrichment analysis of differentially expressed genes (DEGs) revealed, for the first time, that the mechanism of diapause termination in silkworms is lineage-specific. Weighted gene co-expression network analysis (WGCNA) further indicated that this lineage-specific diapause release is primarily driven by the differential expression of genes involved in the carbohydrate biosynthesis pathway. These findings both validate the practical application of electrical stimulation in sericulture and reveal fundamental differences in diapause regulation between silkworm lineages, advancing our understanding of this crucial biological process.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 2","pages":"139-150"},"PeriodicalIF":2.3,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145587378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A basic helix–loop–helix/Per-Arnt-Sim transcription factor Taiman is essential for metamorphosis in Henosepilachna vigintioctomaculata 一种基本的螺旋-环-螺旋/ per - art - sim转录因子Taiman在猕猴桃（Henosepilachna vintioctomaculata）的变态过程中是必不可少的。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2026-03-03 Epub Date: 2025-12-12 DOI: 10.1111/imb.70023

Jia-Qing Yu, Yi-Kuan Wu, Lin Jin, Guo-Qing Li

{"title":"A basic helix–loop–helix/Per-Arnt-Sim transcription factor Taiman is essential for metamorphosis in Henosepilachna vigintioctomaculata","authors":"Jia-Qing Yu, Yi-Kuan Wu, Lin Jin, Guo-Qing Li","doi":"10.1111/imb.70023","DOIUrl":"10.1111/imb.70023","url":null,"abstract":"Insect Taiman (Tai) is a basic helix–loop–helix (bHLH)/Per-Arnt-Sim (PAS) transcription factor that plays a role in juvenile hormone (JH) signalling during the larval stage in various insect species, as well as in 20-hydroxyecdysone (20E) signalling during the oogenesis of Drosophila melanogaster. It forms a heterodimer with another bHLH-PAS transcription factor Methoprene-tolerant (Met) mediating JH signalling and acts as a steroid receptor coactivator of the functional 20E receptor heterodimer, ecdysone receptor (EcR) and ultraspiracle (USP). However, whether Tai plays the dual functions in other insects deserves further exploration. In this study, we determined the role of Tai in Henosepilachna vigintioctomaculata, a Coleopteran potato pest mainly distributed in northern China. HvTai mRNA levels were higher just before and right after the ecdysis within each larval instar and at early and late stages of prepupae than those in the intermediate days. RNA interference (RNAi)-aided knockdown of Tai at the second, penultimate and final larval instar stages caused 100% mortalities 9, 11 and 10 days post dsRNA administration. All Tai RNAi larvae failed to complete moulting; they were wrapped in the old larval exuviae, gradually darkened and shrivelled and ultimately died. The Tai-depleted larvae did not complete gut clearing; the larval guts contained food residues. Moreover, knockdown of Tai disrupted both JH and 20E pathway genes. Our findings demonstrated that Tai was necessary for metamorphosis in H. vigintioctomaculata. The findings also imply that H. vigintioctomaculata is an ideal model for further exploration of how Tai contributes specifically to hormone signalling.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 2","pages":"187-196"},"PeriodicalIF":2.3,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145742318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kr-h1 is vital for vitellogenesis in two ladybird beetle species Kr-h1对两种瓢虫的卵黄形成至关重要。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2026-03-03 Epub Date: 2025-12-12 DOI: 10.1111/imb.70022

Hai-Hui Liu, Xiao-Qing Zhang, Jia-Qing Yu, Lin Jin, Guo-Qing Li

{"title":"Kr-h1 is vital for vitellogenesis in two ladybird beetle species","authors":"Hai-Hui Liu, Xiao-Qing Zhang, Jia-Qing Yu, Lin Jin, Guo-Qing Li","doi":"10.1111/imb.70022","DOIUrl":"10.1111/imb.70022","url":null,"abstract":"Krüppel homolog1 (Kr-h1), a transcription factor with a DNA-binding motif of eight C2H2 zinc fingers, exerts species-specific functions by mediating juvenile hormone (JH) signal in certain physiological processes during female reproduction in insects. Nevertheless, the regulatory role of Kr-h1 on vitellogenesis deserves further exploration. Here, we found that Kr-h1 was actively expressed from 0- to 12-day-old females and among detected tissues including fat body and ovary in two potato-defoliating beetles, Henosepilachna vigintioctopunctata and H. vigintioctomaculata. RNA interference (RNAi) for Kr-h1 significantly lengthened the preoviposition period, dramatically impaired ovarian development, and decreased oviposition. Weaker yolk accumulation occurred in the Kr-h1 RNAi oocytes, in contrast to a larger quantity of yolk granules in controls. Correspondingly, Vg transcript levels in the fat bodies and ovaries and of VgR amounts in the ovarian tissues were substantially reduced in the Kr-h1 knockdown samples. In H. vigintioctomaculata, RNAi for the other two JH signal genes, HvmHairy and HvmGroucho, was also performed. Silencing either or both genes affected neither female reproduction nor the mRNA levels of Vg and VgR. Therefore, our findings suggest Kr-h1, rather than Hairy/Groucho, regulates vitellogenesis in the two Henosepilachna species. Moreover, targeting Kr-h1 could potentially be a new way to control potato pests.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"35 2","pages":"177-186"},"PeriodicalIF":2.3,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145742296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0