Insect Molecular Biology最新文献_第10页

Expansion of the genetic toolbox for manipulation of the global crop pest Drosophila suzukii: Isolation and assessment of eye colour mutant strains 扩大全球作物害虫铃木果蝇的遗传工具箱：眼睛颜色突变株的分离和评估。

IF 2.6 2区农林科学

Insect Molecular Biology Pub Date : 2023-10-11 DOI: 10.1111/imb.12879

Amarish K. Yadav, Ramasamy Asokan, Akihiko Yamamoto, Anandrao A. Patil, Maxwell J. Scott

{"title":"Expansion of the genetic toolbox for manipulation of the global crop pest Drosophila suzukii: Isolation and assessment of eye colour mutant strains","authors":"Amarish K. Yadav, Ramasamy Asokan, Akihiko Yamamoto, Anandrao A. Patil, Maxwell J. Scott","doi":"10.1111/imb.12879","DOIUrl":"10.1111/imb.12879","url":null,"abstract":"Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), commonly called spotted wing Drosophila, is an important agricultural pest recognised worldwide. D. suzukii is a pest of soft-skinned fruits as females can lay eggs in ripening fruit before harvest. While strains for genetic biocontrol of D. suzukii have been made, the development of transgenic D. suzukii strains and their further screening remain a challenge partly due to the lack of phenotypically trackable genetic-markers, such as those widely used with the model genetic organism D. melanogaster. Here, we have used CRISPR/Cas9 to introduce heritable mutations in the eye colour genes white, cinnabar and sepia, which are located on the X, second and third chromosomes, respectively. Strains were obtained, which were homozygous for a single mutation. Genotyping of the established strains showed insertion and/or deletions (indels) at the targeted sites. A strain homozygous for mutations in cinnabar and sepia showed a pale-yellow eye colour at eclosion but darkened to a sepia colour after a week. The fecundity and fertility of some of the cinnabar and sepia strains were comparable with the wild type. Although white mutant males were previously reported to be sterile, we found that sterility is not fully penetrant and we have been able to maintain white-eyed strains for over a year. The cinnabar, sepia and white mutant strains developed in this study should facilitate future genetic studies in D. suzukii and the development of strains for genetic control of this pest.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"33 2","pages":"91-100"},"PeriodicalIF":2.6,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imb.12879","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41199712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An OBP gene highly expressed in non-chemosensory tissues affects the phototaxis and reproduction of Spodoptera frugiperda 在非化学感觉组织中高度表达的OBP基因影响草地贪夜蛾的趋光性和繁殖。

IF 2.6 2区农林科学

Insect Molecular Biology Pub Date : 2023-10-10 DOI: 10.1111/imb.12880

Wei-Kang Han, Feng-Xian Tang, Yang-Yang Yan, Yan Wang, Yi-Xi Zhang, Na Yu, Kan Wang, Ze-Wen Liu

{"title":"An OBP gene highly expressed in non-chemosensory tissues affects the phototaxis and reproduction of Spodoptera frugiperda","authors":"Wei-Kang Han, Feng-Xian Tang, Yang-Yang Yan, Yan Wang, Yi-Xi Zhang, Na Yu, Kan Wang, Ze-Wen Liu","doi":"10.1111/imb.12880","DOIUrl":"10.1111/imb.12880","url":null,"abstract":"Insect odorant binding proteins (OBPs) were initially regarded as carriers of the odorants involved in chemosensation. However, it had been observed that a growing number of OBP genes exhibited broad expression patterns beyond chemosensory tissues. Here, an OBP gene (OBP31) was found to be highly expressed in the larval ventral nerve cord, adult brain and male reproductive organ of Spodoptera frugiperda. An OBP31 knockout strain (OBP31−/−) was generated by CRISPR/Cas9 mutagenesis. For OBP31−/−, the larvae needed longer time to pupate, but there was no difference in the pupal weight between OBP31−/− and wild type (WT). OBP31−/− larvae showed stronger phototaxis than the WT larvae, indicating the importance of OBP31 in light perception. For mating rhythm of adults, OBP31−/− moths displayed an earlier second mating peak. In the cross-pairing of OBP31−/− and WT moths, the mating duration was longer, and hatchability was lower in OBP31−/− group and OBP31+/−♂ group than that in the WT group. These results suggested that OBP31 played a vital role in larval light perception and male reproductive process and could provide valuable insights into understanding the biological functions of OBPs that were not specific in chemosensory tissues.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"33 1","pages":"81-90"},"PeriodicalIF":2.6,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41182492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct roles of the Hox genes Ultrabithorax and abdominal-A in scorpionfly embryonic proleg development Hox基因Ultrabithorax和腹肌-A在蝎子胚胎前体发育中的不同作用。

IF 2.6 2区农林科学

Insect Molecular Biology Pub Date : 2023-10-04 DOI: 10.1111/imb.12878

Bing-Peng Liu, Bao-Zhen Hua

{"title":"Distinct roles of the Hox genes Ultrabithorax and abdominal-A in scorpionfly embryonic proleg development","authors":"Bing-Peng Liu, Bao-Zhen Hua","doi":"10.1111/imb.12878","DOIUrl":"10.1111/imb.12878","url":null,"abstract":"The abdominal appendages of larval insects have a complex evolutionary history of gain and loss, but the regulatory mechanisms underlying the abdominal appendage development remain largely unclear. Here, we investigated the embryogenesis of abdominal prolegs in the scorpionfly Panorpa liui Hua (Mecoptera: Panorpidae) using in situ hybridization and parental RNA interference. The results show that RNAi-mediated knockdown of Ultrabithorax (Ubx) led to a homeotic transformation of the first abdominal segment (A1) into the third thoracic segment (T3) and changed the distributions of the downstream target Distal-less (Dll) expression but did not affect the expression levels of Dll. Knockdown of abdominal-A (abd-A) resulted in malformed segments, abnormal prolegs and disrupted Dll expression. The results demonstrate that the gene Ubx maintains an ancestral role of modulating A1 appendage fate without preventing Dll initiation, and a secondary adaptation of abd-A evolves the ability to specify abdominal segments and proleg identity. We conclude that changes in abdominal Hox gene expression and their target genes regulate abdominal appendage morphology during the evolutionary course of holometabolous larvae.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"33 1","pages":"69-80"},"PeriodicalIF":2.6,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41140579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of first desaturase subfamily genes on fatty acid synthesis, desiccation tolerance and inter-caste nutrient transfer in the termite Coptotermes formosanus 第一去饱和酶亚家族基因对台湾白蚁脂肪酸合成、耐干燥性和种间营养转移的影响。

IF 2.6 2区农林科学

Insect Molecular Biology Pub Date : 2023-09-26 DOI: 10.1111/imb.12877

Danni Xu, Yuxin Tong, Bosheng Chen, Baoling Li, Shengyin Wang, Dayu Zhang

{"title":"The influence of first desaturase subfamily genes on fatty acid synthesis, desiccation tolerance and inter-caste nutrient transfer in the termite Coptotermes formosanus","authors":"Danni Xu, Yuxin Tong, Bosheng Chen, Baoling Li, Shengyin Wang, Dayu Zhang","doi":"10.1111/imb.12877","DOIUrl":"10.1111/imb.12877","url":null,"abstract":"Desaturase enzymes play an essential role in the biosynthesis of unsaturated fatty acids (UFAs). In this study, we identified seven “first desaturase” subfamily genes (Cfor-desatA1, Cfor-desatA2-a, Cfor-desatA2-b, Cfor-desatB-a, Cfor-desatB-b, Cfor-desatD and Cfor-desatE) from the Formosan subterranean termite Coptotermes formosanus. These desaturases were highly expressed in the cuticle and fat body of C. formosanus. Inhibition of either the Cfor-desatA2-a or Cfor-desatA2-b gene resulted in a significant decrease in the contents of fatty acids (C16:0, C18:0, C18:1 and C18:2) in worker castes. Moreover, we observed that inhibition of most of desaturase genes identified in this study had a negative impact on the survival rate and desiccation tolerance of workers. Interestingly, when normal soldiers were reared together with dsCfor-desatA2-b-treated workers, they exhibited higher mortality, suggesting that desaturase had an impact on trophallaxis among C. formosanus castes. Our findings shed light on the novel roles of desaturase family genes in the eusocial termite C. formosanus.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"33 1","pages":"55-68"},"PeriodicalIF":2.6,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41121182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The silk gland proteome of Stenopsyche angustata provides insights into the underwater silk secretion angustata的丝腺蛋白质组提供了对水下丝分泌的深入了解。

IF 2.6 2区农林科学

Insect Molecular Biology Pub Date : 2023-09-23 DOI: 10.1111/imb.12874

Yu-Jun Wang, Qing-Song Liu, Li-Ping Liu, Yi-Chuan Zhang, Shuang Qiu, Wen-Guang Zhang, Jin-Hang Lin, Yu-Ting Qin, Xin Wang, Yue-Ying Wu, Ping Zhao, Qing-You Xia, Ling Ding, Dong-Ying Rong, Hong Wang, Zhao-Ming Dong

{"title":"The silk gland proteome of Stenopsyche angustata provides insights into the underwater silk secretion","authors":"Yu-Jun Wang, Qing-Song Liu, Li-Ping Liu, Yi-Chuan Zhang, Shuang Qiu, Wen-Guang Zhang, Jin-Hang Lin, Yu-Ting Qin, Xin Wang, Yue-Ying Wu, Ping Zhao, Qing-You Xia, Ling Ding, Dong-Ying Rong, Hong Wang, Zhao-Ming Dong","doi":"10.1111/imb.12874","DOIUrl":"10.1111/imb.12874","url":null,"abstract":"Caddisworms (Trichoptera) spin adhesive silks to construct a variety of underwater composite structures. Many studies have focused on the fibroin heavy chain of caddisworm silk and found that it contains heavy phosphorylation to maintain a stable secondary structure. Besides fibroins, recent studies have also identified some new silk proteins within caddisworm silk. To better understand the silk composition and its secretion process, this study reports the silk gland proteome of a retreat-building caddisworm, Stenopsyche angustata Martynov (Trichoptera, Stenopsychidae). Using liquid chromatography tandem mass spectrometry (LC-MS/MS), 2389 proteins were identified in the silk gland of S. angustata, among which 192 were predicted as secreted silk proteins. Twenty-nine proteins were found to be enriched in the front silk gland, whereas 109 proteins were enriched in the caudal silk gland. The fibroin heavy chain and nine uncharacterized silk proteins were identified as phosphorylated proteins. By analysing the sequence of the fibroin heavy chain, we found that it contains 13 Gly/Thr/Pro-rich regions, 12 Val/Ser/Arg-rich regions and a Gly/Arg/Thr-rich region. Three uncharacterized proteins were identified as sericin-like proteins due to their larger molecular weights, signal peptides and repetitive motifs rich in serine. This study provides valuable information for further clarifying the secretion and adhesion of underwater caddisworm silk.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"33 1","pages":"41-54"},"PeriodicalIF":2.6,"publicationDate":"2023-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41127049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockout of nuclear receptor HR38 gene impairs pupal–adult development in silkworm Bombyx mori 核受体HR38基因敲除对家蚕蛹成体发育的影响。

IF 2.6 2区农林科学

Insect Molecular Biology Pub Date : 2023-09-22 DOI: 10.1111/imb.12876

Xinyue Xu, Shangkun Pu, Mouzhen Jiang, Xiaoxuan Hu, Qing Wang, Jun Yu, Jianghong Chu, Guoqing Wei, Lei Wang

{"title":"Knockout of nuclear receptor HR38 gene impairs pupal–adult development in silkworm Bombyx mori","authors":"Xinyue Xu, Shangkun Pu, Mouzhen Jiang, Xiaoxuan Hu, Qing Wang, Jun Yu, Jianghong Chu, Guoqing Wei, Lei Wang","doi":"10.1111/imb.12876","DOIUrl":"10.1111/imb.12876","url":null,"abstract":"Nuclear receptors are ligand-regulated transcription factors that play important role in regulating insect metamorphosis through the ecdysone signalling pathway. In this study, we investigated the nuclear receptor HR38 gene in Bombyx mori (BmHR38), belonging to the NR4A subfamily. BmHR38 mRNA was highly expressed in the head and epidermis at the pupal stage. The expression of the BmHR38 gene was influenced by different doses of 20E at different times. A BmHR38 deletion mutant silkworm was generated using the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system. Compared with the wild-type B. mori, the BmHR38 deletion mutant resulted in abnormal development during the pupal stage, leading to either failed eclosion or the formation of abnormal adult wings. After silencing of BmHR38 in the pupal stage, the phenotype of pupa or moth had no significant change, but it did result in reduced egg production. The mRNA levels of USP, E75 and E74 were significantly increased, while the transcript levels of FTZ-F1 were suppressed after RNA interference. Furthermore, interference with BmHR38 also inhibited the expressions of chitin metabolism genes, including Chs1, Chs2, Chi, Chi-h and CDA. Our results suggest that BmHR38 is essential for pupal development and pupa–adult metamorphosis in B. mori by regulating the expression of NRs and chitin metabolism genes.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"33 1","pages":"29-40"},"PeriodicalIF":2.6,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41130915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and functional analysis of Cochliomyia hominivorax U6 gene promoters 人源性耳蜗瘤病毒U6基因启动子的鉴定和功能分析。

IF 2.6 2区农林科学

Insect Molecular Biology Pub Date : 2023-09-21 DOI: 10.1111/imb.12875

Rossina Novas, Tatiana Basika, Megan E. Williamson, Pablo Fresia, Alejo Menchaca, Maxwell J. Scott

{"title":"Identification and functional analysis of Cochliomyia hominivorax U6 gene promoters","authors":"Rossina Novas, Tatiana Basika, Megan E. Williamson, Pablo Fresia, Alejo Menchaca, Maxwell J. Scott","doi":"10.1111/imb.12875","DOIUrl":"10.1111/imb.12875","url":null,"abstract":"The New World screwworm, Cochliomyia hominivorax, is an obligate parasite, which is a major pest of livestock. While the sterile insect technique was used very successfully to eradicate C. hominivorax from North and Central America, more cost-effective genetic methods will likely be needed in South America. The recent development of CRISPR/Cas9-based genetic approaches, such as homing gene drive, could provide a very efficient means for the suppression of C. hominivorax populations. One component of a drive system is the guide RNA(s) driven by a U6 gene promoter. Here, we have developed an in vivo assay to evaluate the activity of the promoters from seven C. hominivorax U6 genes. Embryos from the related blowfly Lucilia cuprina were injected with plasmid DNA containing a U6-promoter-guide RNA construct and a source of Cas9, either protein or plasmid DNA. Activity was assessed by the number of site-specific mutations in the targeted gene in hatched larvae. One promoter, Chom U6_b, showed the highest activity. These U6 gene promoters could be used to build CRISPR/Cas9-based genetic systems for the control of C. hominivorax.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"32 6","pages":"716-724"},"PeriodicalIF":2.6,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41129383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Male vitellogenin regulates gametogenesis through a testis-enriched big protein in Chrysopa pallens 雄性卵黄蛋白原通过一种富含睾丸的大蛋白来调节淡色金藻的配子发生。

IF 2.6 2区农林科学

Insect Molecular Biology Pub Date : 2023-09-14 DOI: 10.1111/imb.12873

Xiao-ping Liu, Chang-yan Liu, Yan-jiao Feng, Xing-kai Guo, Li-sheng Zhang, Meng-qing Wang, Yu-yan Li, Fan-rong Zeng, Tony Nolan, Jian-jun Mao

{"title":"Male vitellogenin regulates gametogenesis through a testis-enriched big protein in Chrysopa pallens","authors":"Xiao-ping Liu, Chang-yan Liu, Yan-jiao Feng, Xing-kai Guo, Li-sheng Zhang, Meng-qing Wang, Yu-yan Li, Fan-rong Zeng, Tony Nolan, Jian-jun Mao","doi":"10.1111/imb.12873","DOIUrl":"10.1111/imb.12873","url":null,"abstract":"In insects, vitellogenin (Vg) is generally viewed as a female-specific protein. Its primary function is to supply nutrition to developing embryos. Here, we reported Vg from the male adults of a natural predator, Chrysopa pallens. The male Vg was depleted by RNAi. Mating with Vg-deficient male downregulated female Vg expression, suppressed ovarian development and decreased reproductive output. Whole-organism transcriptome analysis after male Vg knockdown showed no differential expression of the known spermatogenesis-related regulators and seminal fluid protein genes, but a sharp downregulation of an unknown gene, which encodes a testis-enriched big protein (Vcsoo). Separate knockdown of male Vg and Vcsoo disturbed the assembly of spermatid cytoplasmic organelles in males and suppressed the expansion of ovary germarium in mated females. These results demonstrated that C. pallens male Vg signals through the downstream Vcsoo and regulates male and female reproduction.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"33 1","pages":"17-28"},"PeriodicalIF":2.6,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10234440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The diapausing mosquito Culex pipiens exhibits reduced levels of H3K27me2 in the fat body 库蚊脂肪体中的 H3K27me2 水平降低。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2023-09-13 DOI: 10.1111/imb.12871

Xueyan Wei, Prabin Dhungana, Cheolho Sim

{"title":"The diapausing mosquito Culex pipiens exhibits reduced levels of H3K27me2 in the fat body","authors":"Xueyan Wei, Prabin Dhungana, Cheolho Sim","doi":"10.1111/imb.12871","DOIUrl":"10.1111/imb.12871","url":null,"abstract":"Culex pipiens, the northern house mosquito, is a major vector of West Nile virus. To survive the severe winter, adult mosquitoes enter a diapause programme. Extended lifespan and an increase in lipid storage are key indicators of diapause. Post-translational modifications to histone proteins impact the expression of genes and have been linked to the lifespan and energy utilisation of numerous insects. Here, we investigated the potential contribution of epigenetic alterations in initiating diapause in this mosquito species. Multiple sequence alignment of H3 sequences from other insect species demonstrates a high conservation of the H3 histone in Cx. pipiens throughout evolution. We then compared the levels of histone methylation in the ovaries and fat body tissues of diapausing and non-diapausing Cx. pipiens using western blots. Our data indicate that histone methylation levels in the ovaries of Cx. pipiens do not change during diapause. In contrast, H3K27me2 levels decrease more than twofold in the fat body of diapausing mosquitoes relative to non-diapausing counterparts. H3K27 methylation plays a crucial role in chromosome activation and inactivation during development in many insect species. This is predominantly governed by polycomb repressor complex 2. Intriguingly, a previous ChIP-seq study demonstrated that the transcription factor FOXO (Forkhead box O) targets the genes that comprise this complex. In addition, H3K27me2 exhibits dynamic abundance throughout the diapause programme in Cx. pipiens, suggesting its potential role in the initial activation of the diapause programme. This study expands our understanding of the relationship between alterations in epigenetic regulation and diapause.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"33 5","pages":"457-466"},"PeriodicalIF":2.3,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10221651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ecdysone-induced microRNA miR-276a-3p controls developmental growth by targeting the insulin-like receptor in Drosophila 脱皮激素诱导的microRNA miR-276a-3p通过靶向果蝇的胰岛素样受体来控制发育生长。

IF 2.6 2区农林科学

Insect Molecular Biology Pub Date : 2023-09-13 DOI: 10.1111/imb.12872

Sojeong Lee, Nayeon Kim, Daegyu Jang, Hee Kyung Kim, Jongin Kim, Ji Won Jeon, Do-Hwan Lim

{"title":"Ecdysone-induced microRNA miR-276a-3p controls developmental growth by targeting the insulin-like receptor in Drosophila","authors":"Sojeong Lee, Nayeon Kim, Daegyu Jang, Hee Kyung Kim, Jongin Kim, Ji Won Jeon, Do-Hwan Lim","doi":"10.1111/imb.12872","DOIUrl":"10.1111/imb.12872","url":null,"abstract":"Animal growth is controlled by a variety of external and internal factors during development. The steroid hormone ecdysone plays a critical role in insect development by regulating the expression of various genes. In this study, we found that fat body-specific expression of miR-276a, an ecdysone-responsive microRNA (miRNA), led to a decrease in the total mass of the larval fat body, resulting in significant growth reduction in Drosophila. Changes in miR-276a expression also affected the proliferation of Drosophila S2 cells. Furthermore, we found that the insulin-like receptor (InR) is a biologically relevant target gene regulated by miR-276a-3p. In addition, we found that miR-276a-3p is upregulated by the canonical ecdysone signalling pathway involving the ecdysone receptor and broad complex. A reduction in cell proliferation caused by ecdysone was compromised by blocking miR-276a-3p activity. Thus, our results suggest that miR-276a-3p is involved in ecdysone-mediated growth reduction by controlling InR expression in the insulin signalling pathway.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":"32 6","pages":"703-715"},"PeriodicalIF":2.6,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10215335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0