Insect Molecular Biology最新文献_第10页

The silk gland proteome of Stenopsyche angustata provides insights into the underwater silk secretion angustata的丝腺蛋白质组提供了对水下丝分泌的深入了解。

IF 2.6 2区农林科学

Insect Molecular Biology Pub Date : 2023-09-23 DOI: 10.1111/imb.12874

Yu-Jun Wang, Qing-Song Liu, Li-Ping Liu, Yi-Chuan Zhang, Shuang Qiu, Wen-Guang Zhang, Jin-Hang Lin, Yu-Ting Qin, Xin Wang, Yue-Ying Wu, Ping Zhao, Qing-You Xia, Ling Ding, Dong-Ying Rong, Hong Wang, Zhao-Ming Dong

{"title":"The silk gland proteome of Stenopsyche angustata provides insights into the underwater silk secretion","authors":"Yu-Jun Wang, Qing-Song Liu, Li-Ping Liu, Yi-Chuan Zhang, Shuang Qiu, Wen-Guang Zhang, Jin-Hang Lin, Yu-Ting Qin, Xin Wang, Yue-Ying Wu, Ping Zhao, Qing-You Xia, Ling Ding, Dong-Ying Rong, Hong Wang, Zhao-Ming Dong","doi":"10.1111/imb.12874","DOIUrl":"10.1111/imb.12874","url":null,"abstract":"Caddisworms (Trichoptera) spin adhesive silks to construct a variety of underwater composite structures. Many studies have focused on the fibroin heavy chain of caddisworm silk and found that it contains heavy phosphorylation to maintain a stable secondary structure. Besides fibroins, recent studies have also identified some new silk proteins within caddisworm silk. To better understand the silk composition and its secretion process, this study reports the silk gland proteome of a retreat-building caddisworm, Stenopsyche angustata Martynov (Trichoptera, Stenopsychidae). Using liquid chromatography tandem mass spectrometry (LC-MS/MS), 2389 proteins were identified in the silk gland of S. angustata, among which 192 were predicted as secreted silk proteins. Twenty-nine proteins were found to be enriched in the front silk gland, whereas 109 proteins were enriched in the caudal silk gland. The fibroin heavy chain and nine uncharacterized silk proteins were identified as phosphorylated proteins. By analysing the sequence of the fibroin heavy chain, we found that it contains 13 Gly/Thr/Pro-rich regions, 12 Val/Ser/Arg-rich regions and a Gly/Arg/Thr-rich region. Three uncharacterized proteins were identified as sericin-like proteins due to their larger molecular weights, signal peptides and repetitive motifs rich in serine. This study provides valuable information for further clarifying the secretion and adhesion of underwater caddisworm silk.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41127049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockout of nuclear receptor HR38 gene impairs pupal–adult development in silkworm Bombyx mori 核受体HR38基因敲除对家蚕蛹成体发育的影响。

IF 2.6 2区农林科学

Insect Molecular Biology Pub Date : 2023-09-22 DOI: 10.1111/imb.12876

Xinyue Xu, Shangkun Pu, Mouzhen Jiang, Xiaoxuan Hu, Qing Wang, Jun Yu, Jianghong Chu, Guoqing Wei, Lei Wang

{"title":"Knockout of nuclear receptor HR38 gene impairs pupal–adult development in silkworm Bombyx mori","authors":"Xinyue Xu, Shangkun Pu, Mouzhen Jiang, Xiaoxuan Hu, Qing Wang, Jun Yu, Jianghong Chu, Guoqing Wei, Lei Wang","doi":"10.1111/imb.12876","DOIUrl":"10.1111/imb.12876","url":null,"abstract":"Nuclear receptors are ligand-regulated transcription factors that play important role in regulating insect metamorphosis through the ecdysone signalling pathway. In this study, we investigated the nuclear receptor HR38 gene in Bombyx mori (BmHR38), belonging to the NR4A subfamily. BmHR38 mRNA was highly expressed in the head and epidermis at the pupal stage. The expression of the BmHR38 gene was influenced by different doses of 20E at different times. A BmHR38 deletion mutant silkworm was generated using the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system. Compared with the wild-type B. mori, the BmHR38 deletion mutant resulted in abnormal development during the pupal stage, leading to either failed eclosion or the formation of abnormal adult wings. After silencing of BmHR38 in the pupal stage, the phenotype of pupa or moth had no significant change, but it did result in reduced egg production. The mRNA levels of USP, E75 and E74 were significantly increased, while the transcript levels of FTZ-F1 were suppressed after RNA interference. Furthermore, interference with BmHR38 also inhibited the expressions of chitin metabolism genes, including Chs1, Chs2, Chi, Chi-h and CDA. Our results suggest that BmHR38 is essential for pupal development and pupa–adult metamorphosis in B. mori by regulating the expression of NRs and chitin metabolism genes.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41130915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and functional analysis of Cochliomyia hominivorax U6 gene promoters 人源性耳蜗瘤病毒U6基因启动子的鉴定和功能分析。

IF 2.6 2区农林科学

Insect Molecular Biology Pub Date : 2023-09-21 DOI: 10.1111/imb.12875

Rossina Novas, Tatiana Basika, Megan E. Williamson, Pablo Fresia, Alejo Menchaca, Maxwell J. Scott

{"title":"Identification and functional analysis of Cochliomyia hominivorax U6 gene promoters","authors":"Rossina Novas, Tatiana Basika, Megan E. Williamson, Pablo Fresia, Alejo Menchaca, Maxwell J. Scott","doi":"10.1111/imb.12875","DOIUrl":"10.1111/imb.12875","url":null,"abstract":"The New World screwworm, Cochliomyia hominivorax, is an obligate parasite, which is a major pest of livestock. While the sterile insect technique was used very successfully to eradicate C. hominivorax from North and Central America, more cost-effective genetic methods will likely be needed in South America. The recent development of CRISPR/Cas9-based genetic approaches, such as homing gene drive, could provide a very efficient means for the suppression of C. hominivorax populations. One component of a drive system is the guide RNA(s) driven by a U6 gene promoter. Here, we have developed an in vivo assay to evaluate the activity of the promoters from seven C. hominivorax U6 genes. Embryos from the related blowfly Lucilia cuprina were injected with plasmid DNA containing a U6-promoter-guide RNA construct and a source of Cas9, either protein or plasmid DNA. Activity was assessed by the number of site-specific mutations in the targeted gene in hatched larvae. One promoter, Chom U6_b, showed the highest activity. These U6 gene promoters could be used to build CRISPR/Cas9-based genetic systems for the control of C. hominivorax.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41129383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Male vitellogenin regulates gametogenesis through a testis-enriched big protein in Chrysopa pallens 雄性卵黄蛋白原通过一种富含睾丸的大蛋白来调节淡色金藻的配子发生。

IF 2.6 2区农林科学

Insect Molecular Biology Pub Date : 2023-09-14 DOI: 10.1111/imb.12873

Xiao-ping Liu, Chang-yan Liu, Yan-jiao Feng, Xing-kai Guo, Li-sheng Zhang, Meng-qing Wang, Yu-yan Li, Fan-rong Zeng, Tony Nolan, Jian-jun Mao

{"title":"Male vitellogenin regulates gametogenesis through a testis-enriched big protein in Chrysopa pallens","authors":"Xiao-ping Liu, Chang-yan Liu, Yan-jiao Feng, Xing-kai Guo, Li-sheng Zhang, Meng-qing Wang, Yu-yan Li, Fan-rong Zeng, Tony Nolan, Jian-jun Mao","doi":"10.1111/imb.12873","DOIUrl":"10.1111/imb.12873","url":null,"abstract":"In insects, vitellogenin (Vg) is generally viewed as a female-specific protein. Its primary function is to supply nutrition to developing embryos. Here, we reported Vg from the male adults of a natural predator, Chrysopa pallens. The male Vg was depleted by RNAi. Mating with Vg-deficient male downregulated female Vg expression, suppressed ovarian development and decreased reproductive output. Whole-organism transcriptome analysis after male Vg knockdown showed no differential expression of the known spermatogenesis-related regulators and seminal fluid protein genes, but a sharp downregulation of an unknown gene, which encodes a testis-enriched big protein (Vcsoo). Separate knockdown of male Vg and Vcsoo disturbed the assembly of spermatid cytoplasmic organelles in males and suppressed the expansion of ovary germarium in mated females. These results demonstrated that C. pallens male Vg signals through the downstream Vcsoo and regulates male and female reproduction.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10234440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The diapausing mosquito Culex pipiens exhibits reduced levels of H3K27me2 in the fat body 库蚊脂肪体中的 H3K27me2 水平降低。

IF 2.3 2区农林科学

Insect Molecular Biology Pub Date : 2023-09-13 DOI: 10.1111/imb.12871

Xueyan Wei, Prabin Dhungana, Cheolho Sim

{"title":"The diapausing mosquito Culex pipiens exhibits reduced levels of H3K27me2 in the fat body","authors":"Xueyan Wei, Prabin Dhungana, Cheolho Sim","doi":"10.1111/imb.12871","DOIUrl":"10.1111/imb.12871","url":null,"abstract":"Culex pipiens, the northern house mosquito, is a major vector of West Nile virus. To survive the severe winter, adult mosquitoes enter a diapause programme. Extended lifespan and an increase in lipid storage are key indicators of diapause. Post-translational modifications to histone proteins impact the expression of genes and have been linked to the lifespan and energy utilisation of numerous insects. Here, we investigated the potential contribution of epigenetic alterations in initiating diapause in this mosquito species. Multiple sequence alignment of H3 sequences from other insect species demonstrates a high conservation of the H3 histone in Cx. pipiens throughout evolution. We then compared the levels of histone methylation in the ovaries and fat body tissues of diapausing and non-diapausing Cx. pipiens using western blots. Our data indicate that histone methylation levels in the ovaries of Cx. pipiens do not change during diapause. In contrast, H3K27me2 levels decrease more than twofold in the fat body of diapausing mosquitoes relative to non-diapausing counterparts. H3K27 methylation plays a crucial role in chromosome activation and inactivation during development in many insect species. This is predominantly governed by polycomb repressor complex 2. Intriguingly, a previous ChIP-seq study demonstrated that the transcription factor FOXO (Forkhead box O) targets the genes that comprise this complex. In addition, H3K27me2 exhibits dynamic abundance throughout the diapause programme in Cx. pipiens, suggesting its potential role in the initial activation of the diapause programme. This study expands our understanding of the relationship between alterations in epigenetic regulation and diapause.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10221651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ecdysone-induced microRNA miR-276a-3p controls developmental growth by targeting the insulin-like receptor in Drosophila 脱皮激素诱导的microRNA miR-276a-3p通过靶向果蝇的胰岛素样受体来控制发育生长。

IF 2.6 2区农林科学

Insect Molecular Biology Pub Date : 2023-09-13 DOI: 10.1111/imb.12872

Sojeong Lee, Nayeon Kim, Daegyu Jang, Hee Kyung Kim, Jongin Kim, Ji Won Jeon, Do-Hwan Lim

{"title":"Ecdysone-induced microRNA miR-276a-3p controls developmental growth by targeting the insulin-like receptor in Drosophila","authors":"Sojeong Lee, Nayeon Kim, Daegyu Jang, Hee Kyung Kim, Jongin Kim, Ji Won Jeon, Do-Hwan Lim","doi":"10.1111/imb.12872","DOIUrl":"10.1111/imb.12872","url":null,"abstract":"Animal growth is controlled by a variety of external and internal factors during development. The steroid hormone ecdysone plays a critical role in insect development by regulating the expression of various genes. In this study, we found that fat body-specific expression of miR-276a, an ecdysone-responsive microRNA (miRNA), led to a decrease in the total mass of the larval fat body, resulting in significant growth reduction in Drosophila. Changes in miR-276a expression also affected the proliferation of Drosophila S2 cells. Furthermore, we found that the insulin-like receptor (InR) is a biologically relevant target gene regulated by miR-276a-3p. In addition, we found that miR-276a-3p is upregulated by the canonical ecdysone signalling pathway involving the ecdysone receptor and broad complex. A reduction in cell proliferation caused by ecdysone was compromised by blocking miR-276a-3p activity. Thus, our results suggest that miR-276a-3p is involved in ecdysone-mediated growth reduction by controlling InR expression in the insulin signalling pathway.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10215335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of genes associated with the silk gland size using multi-omics in silkworm (Bombyx mori) 利用多组学鉴定家蚕丝腺大小的相关基因

IF 2.6 2区农林科学

Insect Molecular Biology Pub Date : 2023-09-07 DOI: 10.1111/imb.12870

Lindan Sun, Binbin Sun, Liang Chen, Qi Ge, Keping Chen

{"title":"Identification of genes associated with the silk gland size using multi-omics in silkworm (Bombyx mori)","authors":"Lindan Sun, Binbin Sun, Liang Chen, Qi Ge, Keping Chen","doi":"10.1111/imb.12870","DOIUrl":"10.1111/imb.12870","url":null,"abstract":"Silk gland size in silkworms (Bombyx mori) affects silk output. However, the molecular mechanisms by which genes regulate silk gland size remain unclear. In this study, silk glands from three pure silkworm strains (A798, A306 and XH) with different silk gland weight phenotypes were compared using transcriptomics and proteomics to identify differentially expressed genes (DEGs) and proteins (DEPs). When comparing A798 to A306 and A798 to XH, 830 and 469 DEGs were up-regulated, respectively. These genes were related to the gene ontology terms, metabolic process, transport activity and biosynthesis process. In addition, 372 and 302 up-regulated differentially expressed proteins were detected in A798 to A306 and A798 to XH, respectively, related to the gene ontology terms, ribosome and protein export, ribosome and polypeptide biosynthesis processes. Moreover, combined transcriptomics, proteomics and weighted correlation network analyses showed that five genes (BGIBMGA002524, BGIBMGA002629, BGIBMGA005659, BGIBMGA005711 and BGIBMGA010889) were significantly associated with the silk gland weight. Reverse Transcription-quantitative real-time Polymerase Chain Reaction (RT-qPCR) and Enzyme linked immunosorbent assay (ELISA) were used to verify the mRNA and protein expression of five genes in the silk glands and tissues of 18 silkworm strains. The results showed that four genes have higher expression levels in heavier silk glands. These genes are associated with glycogen metabolism, fatty acid synthesis and branched chain amino acid metabolism, thus potentially promoting growth and silk protein synthesis. These findings provide valuable insights into the molecular mechanisms underlying the relationship between silk gland weight and silk yield in silkworms.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10174056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ame-miR-980-3p participates in autophagy-mediated midgut remodelling in Apis mellifera via targeting Atg2B Ame-miR-980-3p通过靶向Atg2B参与蜜蜂自噬介导的中肠重塑。

IF 2.6 2区农林科学

Insect Molecular Biology Pub Date : 2023-09-02 DOI: 10.1111/imb.12869

Wen-Feng Chen, Xue-Peng Chi, Hong-Yu Song, Hong-Fang Wang, Ying Wang, Zhen-Guo Liu, Bao-Hua Xu

{"title":"Ame-miR-980-3p participates in autophagy-mediated midgut remodelling in Apis mellifera via targeting Atg2B","authors":"Wen-Feng Chen, Xue-Peng Chi, Hong-Yu Song, Hong-Fang Wang, Ying Wang, Zhen-Guo Liu, Bao-Hua Xu","doi":"10.1111/imb.12869","DOIUrl":"10.1111/imb.12869","url":null,"abstract":"Autophagy is a process that serves to degrade damaged proteins and organelles, thereby promoting cell homeostasis, differentiation, development and survival. Many miRNAs have been found to have regulatory roles in autophagy. In insects, it has been shown that autophagy is involved in hormone-regulated programmed cell death during metamorphic midgut remodelling. However, whether this is also true during the remodelling of the honey bee midgut is unclear. In the present study, we explored the relationship between autophagy and midgut remodelling and sought to identify miRNAs involved in this physiological process. We found that autophagy occurred during midgut remodelling and that the inhibition of autophagy resulted in midgut dysplasia in prepupae. Differentially expressed miRNAs enriched in the autophagy signalling pathway during midgut remodelling were identified by small RNA-seq. Ame-miR-980-3p, which targets the autophagy-related gene Atg2B, was screened out. Furthermore, abnormal expression of ame-miR-980-3p in the pupal stage led to the thinning of the midgut wall of newly emerged bees (NE). When ame-miR-980-3p expression was inhibited, the intestinal villi of NE bees became significantly shorter and sparse, and the lipid signal in the peritrophic matrix of Pb almost disappeared, indicating that the adult midgut was underdeveloped and the lipid absorption ability was weakened. Taken together, ame-miR-980-3p targeted Atg2B to participate in the regulation of midgut autophagy in the pupae, and the abnormal expression of ame-miR-980-3p would interfere with cell proliferation and death in the process of midgut remodelling, hinder the formation of adult midgut and eventually lead to adult midgut dysplasia and affect the lipid absorption function of the midgut in Apis mellifera.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10492473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockdown of the ecdysone receptor disrupts development and causes mortality in the melon fly, Zeugodacus cucurbitae 蜕皮激素受体的敲低会破坏瓜蝇的发育并导致死亡。

IF 2.6 2区农林科学

Insect Molecular Biology Pub Date : 2023-08-30 DOI: 10.1111/imb.12867

Shakil Ahmad, Momana Jamil, Coline C. Jaworski, Yuejie Wu, Valeria Palma-Onetto, Baoqian Lyu, Yanping Luo

{"title":"Knockdown of the ecdysone receptor disrupts development and causes mortality in the melon fly, Zeugodacus cucurbitae","authors":"Shakil Ahmad, Momana Jamil, Coline C. Jaworski, Yuejie Wu, Valeria Palma-Onetto, Baoqian Lyu, Yanping Luo","doi":"10.1111/imb.12867","DOIUrl":"10.1111/imb.12867","url":null,"abstract":"Cucurbits are important economic plants that are attacked by numerous pests, among which the melon fly Zeugodacus cucurbitae is extremely problematic. New sustainable pest control strategies are necessary to replace chemical insecticides that are harmful to the environment, human health and nontarget species. The RNA interference (RNAi) technology is one of the most promising tools due to high efficiency and species specificity. We developed an RNAi strategy targeting the ecdysone receptor (ECR) of Z. cucurbitae, which plays an important role in moulting and reproduction. We identified, described and isolated the ECR gene of Z. cucurbitae and measured its expression pattern across developmental stages and tissues. ZcECR knockdown via dsZcECR ingestion caused a significant larval mortality and abnormal phenotypes in pupae and adults. About 68% of larvae fed with a dsZcECR-treated diet failed to enter the pupal stage and died. In addition, ZcECR knockdown dramatically reduced pupal weight (by 3.24 mg on average) and fecundity (by about 23%). RNAi targeting the ECR gene is therefore a promising method to control Z. cucurbitae, paving the way for the development of novel sustainable and highly specific control strategies.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10112996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The spread of satellite DNAs in euchromatin and insights into the multiple sex chromosome evolution in Hemiptera revealed by repeatome analysis of the bug Oxycarenus hyalinipennis 准染色质中卫星dna的分布及其对半翅目昆虫多性染色体进化的启示。

IF 2.6 2区农林科学

Insect Molecular Biology Pub Date : 2023-08-24 DOI: 10.1111/imb.12868

Diogo C. Cabral-de-Mello, Pablo Mora, José M. Rico-Porras, Ana B. S. M. Ferretti, Teresa Palomeque, Pedro Lorite

{"title":"The spread of satellite DNAs in euchromatin and insights into the multiple sex chromosome evolution in Hemiptera revealed by repeatome analysis of the bug Oxycarenus hyalinipennis","authors":"Diogo C. Cabral-de-Mello, Pablo Mora, José M. Rico-Porras, Ana B. S. M. Ferretti, Teresa Palomeque, Pedro Lorite","doi":"10.1111/imb.12868","DOIUrl":"10.1111/imb.12868","url":null,"abstract":"Satellite DNAs (satDNAs) are highly repeated tandem sequences primarily located in heterochromatin, although their occurrence in euchromatin has been reported. Here, our aim was to advance the understanding of satDNA and multiple sex chromosome evolution in heteropterans. We combined cytogenetic and genomic approaches to study, for the first time, the satDNA composition of the genome in an Oxycarenidae bug, Oxycarenus hyalinipennis. The species exhibits a male karyotype of 2n = 19 (14A + 2 m + X1X2Y), with a highly differentiated Y chromosome, as demonstrated by C-banding and comparative genomic hybridization, revealing an enrichment of repeats from the male genome. Additionally, comparative analysis between males and females revealed that the 26 identified satDNA families are significantly biased towards male genome, accumulating in discrete regions in the Y chromosome. Exceptionally, the OhyaSat04-125 family was found to be distributed virtually throughout the entire extension of the Y chromosome. This suggests an important role of satDNA in Y chromosome differentiation, in comparison of other repeats, which collectively shows similar abundance between sexes, about 50%. Furthermore, chromosomal mapping of all satDNA families revealed an unexpected high spread in euchromatic regions, covering the entire extension, irrespective of their abundance. Only discrete regions of heterochromatin on the Y chromosome and of the m-chromosomes (peculiar chromosomes commonly observed in heteropterans) were enriched with satDNAs. The putative causes of the intense enrichment of satDNAs in euchromatin are discussed, including the possible existence of burst cycles similar to transposable elements and as a result of holocentricity. These data challenge the classical notion that euchromatin is not enriched with satDNAs.","PeriodicalId":13526,"journal":{"name":"Insect Molecular Biology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10116463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1