In vivo最新文献

{"title":"Differential Expression of NRF2 in the Cortex and Hippocampus Following Bilateral Common Carotid Artery Occlusion.","authors":"Min Seon Choi, Hyo-In Hwang, Yoon Young Chung, Hye-Kyoung Shin, Dong-Joon Kim, Yong Hyun Jun","doi":"10.21873/invivo.13878","DOIUrl":"10.21873/invivo.13878","url":null,"abstract":"<p><strong>Background/aim: </strong>Vascular dementia is the second most common cognitive disorder after Alzheimer's disease. Bilateral common carotid artery occlusion (BCCAO) is a widely used model of vascular dementia associated with chronic cerebral hypoperfusion. Previous studies have reported a beneficial role of nuclear factor erythroid 2-related factor 2 (NRF2) in BCCAO. This study aimed to investigate NRF2 expression in the cortex and hippocampus at 3 and 14 days after BCCAO.</p><p><strong>Materials and methods: </strong>Unoperated male Sprague-Dawley rats were assigned to the control group, while rats that underwent surgery were assigned to the BCCAO group. The right and left common carotid arteries were exposed beneath the esophagus, separated from the vagus nerve and occluded using 4-0 silk sutures. The cerebral cortex and hippocampus were isolated under anesthesia, 3 and 14 days post-surgery. The expression of NRF2 protein was evaluated using western blot analysis.</p><p><strong>Results: </strong>NRF2 expression in the cerebral cortex increased 3 and 14 days after BCCAO, compared to control group. In the hippocampus, NRF2 expression of BCCAO group mice was increased at 3 days, but no difference was observed at day 14 compared to the control group.</p><p><strong>Conclusion: </strong>Chronic hypoperfusion induced by BCCAO altered the protein expression levels of NRF2 in the cortex and hippocampus, suggesting that NRF2 may have a role in cognitive impairment.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"742-748"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Secretome from HMGB1 Box A-over-expressing Adipose-derived Stem Cells Shows Potential for Skin Rejuvenation by Senescence Reversal in PM2.5-induced Senescence Cells <i>via</i> Stem Cell Induction.","authors":"Zin Zin Ei, Apiwat Mutirangura, Pithi Chanvorachote","doi":"10.21873/invivo.13881","DOIUrl":"10.21873/invivo.13881","url":null,"abstract":"<p><strong>Background/aim: </strong>Exposure to particulate matter 2.5 (PM2.5) can lead to cellular senescence by generating reactive oxygen species (ROS). Box A, a DNA-binding domain found in HMGB1, is known for its ability to counteract aging characteristics. This study explored whether BoxA-induced adipose-derived stem cells secretome (BoxA-SC) can reverse senescence in DP and HWPc cells.</p><p><strong>Materials and methods: </strong>The stemness characteristics and reversal of senescence by BoxA-SC in PM2.5-induced DP and HWPc cells were assessed at the mRNA level using RT-qPCR and at the protein level using immunofluorescence analysis.</p><p><strong>Results: </strong>BoxA-SC (1:20) treatment for 48 h induced stemness and reversed PM2.5-induced cell senescence in DP and HWPc cells. BoxA-SC significantly reduced senescence markers, including SA-β-gal staining, and decreased mRNA levels of senescence-associated secretory phenotype factors (IL1α, IL7, CXCL1) in PM2.5-induced senescent cells. DP and HWPc cells exposed to PM2.5 exhibited an increase in p21 and p16 mRNA and protein levels, which was reversed by BoxA-SC. BoxA-SC reduced p21 and p16 in DP senescent cells approximately 3- and 2-fold, respectively, compared to untreated senescent cells.</p><p><strong>Conclusion: </strong>BoxA-SC can potentially reverse cellular senescence, highlighting the therapeutic potential of stem cells in skin rejuvenation and anti-aging treatments.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"766-777"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884463/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short- and Long-term Surgical Results of Extended Surgery for Widespread Gallbladder Carcinoma.","authors":"Takehiro Noji, Shintaro Takeuchi, Masataka Wada, Kimitaka Tanaka, Aya Matsui, Yoshitsugu Nakanishi, Toshimichi Asano, Toru Nakamura, Yasuyuki Kawamoto, Satoshi Hirano","doi":"10.21873/invivo.13907","DOIUrl":"10.21873/invivo.13907","url":null,"abstract":"<p><strong>Background/aim: </strong>Hepatectomy with extrahepatic bile duct resection (Hx+EBDR), pancreaticoduodenectomy (PD), and occasionally hepatopancreaticoduodenectomy (HPD) are required for the treatment of advanced gallbladder cancer (GBC). This study aimed to clarify the clinical value of these extended surgeries for GBC.</p><p><strong>Patients and methods: </strong>We retrospectively reviewed the medical records of patients who underwent curative resection (Surg-G, n=59), and their survival rates were compared with those of patients with unresectable GBC who underwent chemotherapy (CTx-G, n=63).</p><p><strong>Results: </strong>We performed PD (n=15), Hx+EBDR (n=37), and HPD (n=7). The postoperative complication and death rates were as follows: PD, 40% and 7%, respectively; Hx+EBDR, 89% and 14%, respectively; and HPD, 57% and 0%, respectively. Concomitant vascular resection (VR) was required in 61% of the patients. The 5-year overall survival rate and median survival time (MST) for Surg-G were 25.1% and 26 months, respectively, whereas those for CTx-G were 4.6% and 14.4 months, respectively. There were no significant differences between the surgical procedures. Patients who underwent VR had similar prognoses (5-year overall survival rate and MST: 14.5% and 22.3 months, respectively) as the patients in CTx-G.</p><p><strong>Conclusion: </strong>Although extended surgery may be considered for patients with GBC, careful patient selection and new therapeutic strategies are required, especially for those requiring VR.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"1022-1032"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884480/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HT1080 Fibrosarcoma With Acquired Trabectedin Resistance: Increased Malignancy But Sustained Sensitivity to Methionine Restriction.","authors":"Sei Morinaga, Qinghong Han, Kohei Mizuta, Byung Mo Kang, Michael Bouvet, Norio Yamamoto, Katsuhiro Hayashi, Hiroaki Kimura, Shinji Miwa, Kentaro Igarashi, Takashi Higuchi, Hiroyuki Tsuchiya, Satoru Demura, Robert M Hoffman","doi":"10.21873/invivo.13872","DOIUrl":"10.21873/invivo.13872","url":null,"abstract":"<p><strong>Background/aim: </strong>Trabectedin is a DNA-binding agent that has shown moderate efficacy for soft-tissue sarcomas. We have previously shown that methionine restriction enhances trabectedin efficacy on both parental and trabectedin-resistant HT1080 (TR-HT1080) cells <i>in vitro</i> The aim of the present study was to determine whether fibrosarcoma cells that acquire trabectedin resistance become more malignant but maintain sensitivity to methionine restriction <i>in vivo</i>Materials and Methods: TR-HT1080 was established by culturing HT1080 cells in stepwise increasing concentrations of trabectedin. An <i>in vitro</i> wound-healing invasion assay was used to compare malignancy of HT1080 and TR-HT1080. <i>In vivo</i>, six groups were established: G1-G4 (TR-HT1080): G1, untreated control; G2, trabectedin treatment; G3, methionine-restricted diet; G4, methionine-restricted diet combined with trabectedin; G5, untreated control of parental HT1080; and G6, trabectedin treatment of parental HT1080.</p><p><strong>Results: </strong>The IC₅₀ of trabectedin was previously determined to be 3.3 nM for the parental HT1080 cells and 42.9 nM for trabectedin-resistant HT1080 cells, representing a 13-fold increase. Wound-healing invasion assays <i>in vitro</i> showed a more rapid wound-closure ratio in TR-HT1080 cells than in parental cells, suggesting increased malignancy compared to the parental cells. The volume of untreated TR-HT1080 tumors grew more rapidly than that of HT1080 tumors, indicating a higher malignancy of TR-HT1080 tumors. The IC₅₀ of recombinant methioninase was previously determined as 0.75 U/ml for HT1080 and 0.93 U/ml for TR-HT1080 cells. Methionine restriction was highly effective on TR-HT1080 tumors, decreasing tumor growth by 4-fold.</p><p><strong>Conclusion: </strong>TR-HT1080 cells acquired high malignancy by <i>in vitro</i> selection for trabectedin resistance. However, methionine restriction overcame trabectedin resistance <i>in vivo</i>, strongly inhibiting tumor growth, which should be further investigated in the clinic.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"683-690"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility of Coplanar VMAT for Brain Metastases Using Halcyon With Knowledge-based Planning from Non-coplanar Plan.","authors":"Yusuke Sakai, Kazuki Kubo, Kenji Matsumoto, Makoto Hosono, Hajime Monzen","doi":"10.21873/invivo.13893","DOIUrl":"10.21873/invivo.13893","url":null,"abstract":"<p><strong>Background/aim: </strong>This study aimed to clarify the feasibility of creating coplanar single-isocenter volumetric modulated arc therapy (SI-VMAT) with a Halcyon using a knowledge-based planning system based on non-coplanar plans for treating multiple brain metastases.</p><p><strong>Patients and methods: </strong>A knowledge-based RapidPlan (RP) planning model was built using 32 TrueBeam SI-VMAT plans including one full arc and three non-coplanar partial arcs. To confirm its applicability across different beam geometries using the Halcyon system, the model was validated on the basis of the root-mean-square error (RSME), deviation rate, and absolute differences between estimated and actual dose-volume histograms (DVHs). Dosimetric performance of TrueBeam and Halcyon plans was then compared for 10 patients.</p><p><strong>Results: </strong>The average RSME values for the Halcyon and TrueBeam plans were 0.75±0.40 Gy and 0.46±0.28 Gy, respectively (<i>p</i><0.05). Using the model for Halcyon plan creation, deviations from the estimated DVH boundaries were mainly observed in the low-dose region (<9 Gy), while the actual DVH closely aligned with the estimated DVH in the intermediate- and higher-dose regions. For comparison of dosimetric performance, statistically significant differences were found in the gradient index, conformity index, and normal brain volumes receiving ≥12 Gy, ≥18 Gy, and ≥27 Gy. However, these differences were deemed clinically negligible, with less than 1% disparity in the above doses to the normal brain.</p><p><strong>Conclusion: </strong>The RP model can effectively predict doses for Halcyon, regardless of different beam configurations. Coplanar SI-VMAT with Halcyon can feasibly be used in clinical applications, such as the treatment of multiple brain metastases.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"894-901"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884445/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ball Tip Feeler <i>vs.</i> Depth Gauge: Detection of Bony Pedicle Defects Before Pedicle Screw Insertion.","authors":"Ulf Brunnemer, Sabine Roth, Christian W Müller, Tobias Hüfner, Sebastian Decker","doi":"10.21873/invivo.13876","DOIUrl":"10.21873/invivo.13876","url":null,"abstract":"<p><strong>Background/aim: </strong>The objective of this study was to investigate whether the detection rates of pedicle breaches depended on the surgeon's experience level and whether different instruments resulted in varied palpation quality.</p><p><strong>Materials and methods: </strong>Experienced surgeons (ES) (n=8) and inexperienced surgeons (IS) (n=10) were compared. The study was performed using a sawbone model of the spine. Pedicle defects were created at various positions and levels. Participants detected and located the bony defects using a depth gauge and a straight ball tip feeler. After the first measurement, the IS group underwent training focused on identifying bone defects. The experiment was repeated after three weeks under identical conditions.</p><p><strong>Results: </strong>A significant difference was found between ES and IS in the time required to palpate pedicles and bony defects using the ball tip feeler during the first measurement (297.2±114.4 s <i>vs.</i> 202.1±77.9 s; <i>p</i>=0.05). However, after training and during the second measurement three weeks later, these differences were no longer observed (223.7±65.1 s <i>vs.</i> 212.2±73.6 s; <i>p</i>=0.73). Notably, no significant differences were found in the accuracy in detecting bony pedicle defects between the two groups, regardless of the device used. Furthermore, no improvement was found in the IS group after training, regardless of the device used.</p><p><strong>Conclusion: </strong>ES and IS accurately detected pedicle breaches without significant differences. Training did not affect detection rates between the groups, and the choice of device did not affect the accuracy of pedicle breach detection.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"724-731"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boron-dipyrromethene Staining May Enhance Fat Detection in the MASLD Zebrafish Model: NGS-validated lncRNA Profiling.","authors":"Wookjae Jung, Min Hye Kim, Jung Wook Yang, Dong Chul Kim, Jong Sil Lee, Jeong-Hee Lee, Hyo Jung An, Dae Hyun Song","doi":"10.21873/invivo.13879","DOIUrl":"10.21873/invivo.13879","url":null,"abstract":"<p><strong>Background/aim: </strong>Metabolic dysfunction-associated steatotic liver disease (MASLD) is a serious global public health concern. Long non-coding RNAs (lncRNAs) have been identified as key contributors to MASLD pathogenesis. Zebrafish can be utilized to study the relationship between MASLD and lncRNAs because of their similarity to human genes. Oil Red O staining is a traditional method for confirming liver fatty changes; however, it has several limitations. This study aimed to evaluate the efficacy of boron-dipyrromethene (BODIPY) in detecting fatty changes in the liver.</p><p><strong>Materials and methods: </strong>Liver tissues were collected from 30 zebrafish that were fed a BODIPY-containing high-cholesterol diet. Oil Red O and BODIPY staining were evaluated by two pathologists, and next-generation sequencing (NGS) was performed using liver tissues categorized into high fatty change (six liver tissues) and low fatty change (six liver tissues) groups.</p><p><strong>Results: </strong>BODIPY and Oil Red O staining of zebrafish liver sections correlated significantly (<i>p</i>=0.009). NGS identified eight differentially expressed lncRNAs with over a 10-fold difference between the high- and low-fatty acid change groups. Of these, three showed lncRNA-mRNA interaction networks linked to human disorders.</p><p><strong>Conclusion: </strong>BODIPY staining is a reliable alternative to Oil Red O staining for assessing fatty changes in MASLD zebrafish models, particularly when examining frozen liver sections.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"749-757"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884486/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Hypoxia on Irisin Secretion by Human Cardiomyocytes.","authors":"Maciej Grzeszczuk, Urszula Ciesielska, Agnieszka Rusak, Michał Jerzy Kulus, Katarzyna Haczkiewicz-Leśniak, Karolina Jabłońska, Alicja Kmiecik, Marzenna Podhorska-Okołów, Piotr Dzięgiel, Katarzyna Nowińska","doi":"10.21873/invivo.13864","DOIUrl":"10.21873/invivo.13864","url":null,"abstract":"<p><strong>Background/aim: </strong>Cardiovascular disease (CVD) is the leading cause of death worldwide, accounting for 31% of all deaths. Biomarkers such as troponins and natriuretic peptides are crucial in diagnosing CVD. Recently, irisin (Ir), a myokine derived from the cleavage of fibronectin type III domain-containing protein 5 (FNDC5), has been identified as a potential new biomarker for CVD. Ir is involved in regulating energy metabolism. This study aimed to determine the expression levels of the <i>FNDC5</i> gene and the level of Ir in cardiomyocytes of the AC16 line subjected to hypoxia.</p><p><strong>Materials and methods: </strong>AC16 cardiomyocytes were cultured under hypoxic conditions for two, four, and six hours. Molecular studies were conducted using western blot, immunofluorescence, RT-PCR, immunoenzymatic test (ELISA), and electron microscopy methods.</p><p><strong>Results: </strong><i>FNDC5</i> gene expression was significantly elevated in cells subjected to hypoxia. Additionally, Ir levels increased in the first hours of hypoxia.</p><p><strong>Conclusion: </strong>Ir could be a potentially useful indicator for assessing CVD risk. Further research is needed to confirm whether elevated Ir levels under hypoxic conditions in AC16 cells represent a promising direction for the development of biomarkers for CVD.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"656-668"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884492/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Diode Laser Irradiation on Bone Formation in the Tibiae of Estrogen-deficient Rats.","authors":"Toshiko Furukawa, Munemitsu Kawano, Satoshi Yokose, Rina Iwamoto, Nobuyuki Udagawa","doi":"10.21873/invivo.13868","DOIUrl":"10.21873/invivo.13868","url":null,"abstract":"<p><strong>Background/aim: </strong>The aim of this study was to examine the effects of diode laser irradiation (910 nm) on bone formation in tibiae with bone defects in estrogen-deficient rats. Micro-computed tomography (micro-CT) was performed for 3-dimensional (3D) morphological evaluation of newly formed bone tissues.</p><p><strong>Materials and methods: </strong>Rats underwent sham operation (Sham) or ovariectomy (OVX), and bone defects were created in the tibiae, which were then subjected to diode laser irradiation for seven days in the laser groups (Sham-laser or OVX-laser groups). Tibiae with bone defects from Sham or OVX groups were irradiated with a guide light instead of a laser, serving as control groups (Sham-Cont or OVX-Cont groups). The tibiae were exposed to laser irradiation every day over a period of seven days. After irradiation, the tibiae underwent micro-CT, and then 3D reconstruction was performed for analysis of new bone formation.</p><p><strong>Results: </strong>Estrogen deficiency induced osteoporosis in the tibiae of OVX rats. Laser irradiation induced greater new bone formation in the region of bone defects in both Sham- and OVX-laser groups compared to the Sham- and OVX-Cont groups, respectively. No significant difference in the volume of new bone formation was seen between Sham- and OVX-laser groups.</p><p><strong>Conclusion: </strong>Laser irradiation could induce new bone formation in the region of bone defects in both Sham and OVX rats. This suggests that laser irradiation has potential for bone regeneration therapy in cases of postmenopausal osteoporosis.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"634-639"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-term Efficacy of Botulinum Toxin A for Refractory Overactive Bladder in Female Patients.","authors":"Hirotaka Sato, Takahiro Watanabe, Shota Otsuka, Sachiyuki Tsukada","doi":"10.21873/invivo.13899","DOIUrl":"10.21873/invivo.13899","url":null,"abstract":"<p><strong>Background/aim: </strong>This study aimed to assess the effectiveness and safety of 100 U onabotulinumtoxinA (also known as botulinum toxin type A) for overactive bladder (OAB) and urgency urinary incontinence (UUI) in Japanese women.</p><p><strong>Patients and methods: </strong>This prospective, nonrandomized study was conducted as a single-center clinical trial in Japan and targeted patients with OAB that did not respond adequately to standard treatments, including anticholinergics and β3-adrenergic receptor agonists. A total of 26 patients received a single intradetrusor injection of onabotulinumtoxinA. The primary endpoint was the change in the number of daily UUI episodes between baseline and week 12. The secondary endpoints were improvements in other OAB symptoms and health-related quality of life (HRQoL). Adverse events were monitored.</p><p><strong>Results: </strong>Patients who received onabotulinumtoxinA experienced a significant reduction in the mean number of daily UUI episodes between baseline and week 12 (-3.49; <i>p</i><0.001). Significant improvements in all secondary endpoints except the personal relationships domain of King's Health Questionnaire were observed. Adverse events, such as urinary tract infection, cystitis, dysuria, and increased postvoid residual urine volume, occurred more frequently with onabotulinumtoxinA. However, the severity of most adverse events was mild or moderate.</p><p><strong>Conclusion: </strong>OnabotulinumtoxinA administered at a dose of 100 U was well-tolerated and resulted in significant and clinically meaningful improvements in the OAB symptoms and HRQoL of patients whose OAB could not be adequately managed with other treatments.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 2","pages":"942-950"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}