In vivo最新文献

{"title":"Transcriptome Analysis of the Effects of X-Ray Radiotherapy on Non-small-cell Lung Cancer Using Next-generation Sequencing.","authors":"Serhat Aras, Tuğba Kul Köprülü, Burçin Erkal Çam, Jülide Balkan, Esra Erdem, Esra Çikler, Altan Kara","doi":"10.21873/invivo.14070","DOIUrl":"https://doi.org/10.21873/invivo.14070","url":null,"abstract":"<p><strong>Background/aim: </strong>This study investigated the acute effects of flattening filter (FF) and flattening filter-free (FFF) beams on gene expression in non-small-cell lung cancer (NSCLC).</p><p><strong>Materials and methods: </strong>Thirty-six adult athymic nude mice were divided into five groups. The control group did not undergo any radiotherapy or treatment procedures, whereas in the lung cancer (LCa) group, a cancer model was created but not irradiated. LCa models received 20 Gy radiotherapy with FF at 400 MU/min, or with FFF at 1,000 or 1,800 MU/min dose rates. The mice were irradiated 20 days after A549 cancer cell-line implantation and sacrificed 48 h after irradiation for genetic analysis.</p><p><strong>Results: </strong>Twelve genes were identified as being common across all radiotherapy groups. The expression of most of these genes changed as the dose rate increased. Seven of these genes were also common to the LCa and control groups. Three genes down-regulated in the untreated cancer group showed increased expression with higher dose rates in treated groups. Significant differences were observed in glutamatergic synapse, actin cytoskeleton regulation, and steroid synthesis in FF-400 and FFF-1000. The FFF-1800 group exhibited significant changes in RNA transport, actin cytoskeleton regulation, and phagosome-associated pathways.</p><p><strong>Conclusion: </strong>FFF beams induced more extensive and pronounced gene-expression changes compared to FF beams in NSCLC.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 5","pages":"2711-2727"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Real Anatomy of Mandibular Canal: \"New Features and Prospectives in Oral and Maxillofacial Surgery\".","authors":"Dardo Menditti, Vittorio Salvatore Menditti, Martina Amodio, Giovanna Cataldo, Marco Menditti, Alfonso Baldi","doi":"10.21873/invivo.14088","DOIUrl":"https://doi.org/10.21873/invivo.14088","url":null,"abstract":"<p><strong>Background/aim: </strong>The anatomy of the mandibular canal (MC) has been extensively described in the literature. Numerous studies have investigated its anatomical variations and anomalies. Different methods have been used to study the mandibular bone over the years and different classifications have been proposed.</p><p><strong>Patients and methods: </strong>A literature search was performed to identify articles regarding Mandibular Canal, Bifid and Trifid Mandibular Canal. This search used the PUBMED and WoS computer databases in the years from 1931 to 2023. Studies that did not fall within the scope of the search were excluded, such as those in which the abstract provided insufficient information for the preliminary evaluation. In addition, some articles not selected by the database search, including those on etiopathogenesis were manually entered. For all studies considered eligible, the full texts of the articles were searched and downloaded.</p><p><strong>Results: </strong>Based on the analysis of the literature, the variants of the MC should be classified as bifid MC, trifid MC, intramandibular accessory canals and dental canals. Extramandibular buccal and lingual canals require focused research, because they are very frequent, numerous, and they have nutrient functions.</p><p><strong>Conclusion: </strong>This article highlights some important radiological and anatomic aspects regarding variations of the MC and their clinical implications in relation to their actual classification. Some classifications have only statistical and historical value without real utility for dentists and oral surgeons. The presence of duplications may cause perioperative complications if they are not correctly recognized. New studies should be conducted on the different variations of MC in its anterior, middle and posterior. A more useful classification is proposed.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 5","pages":"2883-2892"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In Vitro, Ex Vivo</i> and <i>In Vivo</i> Characterization of Cytocompatibility and Biocompatibility of a Cross-linked Fish Collagen Wound Membrane.","authors":"Ole Jung, Kim Burckhardt, Sebastian Stammkoetter, Marina Macias Barrientos, Cindy Reinholdt, Tonya Andreeva, Milena Radenković Stošić, Sanja Stojanović, Stevo Najman, Rumen Krastev, Frank Walther, Sven Pantermehl, Mike Barbeck","doi":"10.21873/invivo.14051","DOIUrl":"https://doi.org/10.21873/invivo.14051","url":null,"abstract":"<p><strong>Background/aim: </strong>The aim of the present research is a comprehensive evaluation of a fish-collagen based wound membrane using established <i>ex vivo, in vitro</i> and <i>in vivo</i> methodologies. A porcine pericardium membrane served as control material.</p><p><strong>Materials and methods: </strong>Scanning electron microscopy (SEM) and attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) analysis were initially used to analyze the structure and collagen molecular structure. Also, a comparison of protein adsorption <i>via</i> measurement of human serum albumin (HSA) adsorption was conducted. The membrane influence on cell viability, cell proliferation as well as their cytotoxic potential were examined <i>in vitro</i>. Additionally, the membrane tissue integration, degradation behavior and biocompatibility were investigated using the subcutaneous implantation model.</p><p><strong>Results: </strong>The SEM analysis showed differences in the structure and the porosity of both membranes. The analysis <i>via</i> FTIR spectroscopy revealed that collagen molecules are present in both membranes in their triple helical structure. The adsorption measurements showed that the surface density of HSA adsorbed to the fish collagen membrane surfaces was significantly lower compared to the values measured for the bovine pericardium membrane. Furthermore, both membranes demonstrated sufficient <i>in vitro</i> cytocompatibility in the indirect colorimetric XTT, LDH and BrdU assays. The <i>in vivo</i> study part revealed that the fish collagen membrane induced a faster biodegradation and a more pronounced pro-inflammatory tissue response, whereas the bovine pericardium membrane degrades more slowly.</p><p><strong>Conclusion: </strong>The results of this study highlight the potential of fish collagen membranes as biocompatible wound healing materials. However, their rapid degradation presents a challenge that needs to be addressed through targeted modifications, such as optimized cross-linking.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 5","pages":"2517-2533"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative Analysis of CD27/CD70 Protein Expression and Investigation of Related Mechanisms in the Tumor Microenvironment of NSCLC.","authors":"Merve Saide Uzunoğlu, Aylin Seher Uzunoğlu, Akif Turna, Volkan Kara, Özlem Küçükhüseyin, Cem Horozoğlu, Ilhan Yaylim","doi":"10.21873/invivo.14066","DOIUrl":"https://doi.org/10.21873/invivo.14066","url":null,"abstract":"<p><strong>Background/aim: </strong>Lung cancer is among the cancers with the highest mortality rates worldwide. Biomarkers associated with it are critically important for diagnosis, evaluation of clinicopathological features, and the development of treatment strategies.</p><p><strong>Patients and methods: </strong>In this study, a total of 32 NSCLC primary tumor tissues and 32 matching 32 normal tissues were analyzed with Western Blot for CD70, CD27, CD3, and FOXP3; positive and negative results were evaluated according to a quantitative analysis of expression with ImageJ. Furthermore, the levels of sCD27 in the serum samples of 30 NSCLC patients and 32 healthy controls were investigated by ELISA.</p><p><strong>Results: </strong>CD70 expression was observed in 5/32 (15.63%) NSCLC tumors, CD27 in 24/32 (75%), CD3 in 28/32 (87.5%), and FOXP3 in 14/32 (43.75%) tumor tissues, all significantly differing from normal tissues (<i>p</i><0.0001). The mean serum sCD27 level in NSCLC patients (117.29±38.18 U/ml) was considerably higher than in controls (<i>p</i><0.0001). Positive CD70 expression in tumors was significantly correlated with higher serum sCD27 levels compared to CD70-negative tumors (<i>x</i>0.007). No significant association was found between overall survival and sCD27 status (<i>p</i>=0.779).</p><p><strong>Conclusion: </strong>The findings suggest that the CD27/CD70 pathway is a potential diagnostic and therapeutic target in NSCLC. Serum sCD27 levels may serve as a diagnostic biomarker. CD70, CD27, CD3 and FOXP3 quantifications show that CD70 is expressed in NSCLC, and related molecular mechanisms support previous findings. However, further studies are required with larger cohorts in NSCLC.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 5","pages":"2659-2668"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396056/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Anticholinergic Drug Use and Febrile Neutropenia Induced by Anticancer Chemotherapy: Analysis of the Japanese Adverse Drug Event Report Database.","authors":"Anna Kato-Ogiso, Tomohiro Mizuno, Koki Kato, Fumihiro Mizokami, Sho Hasegawa, Tsuyoshi Nakai, Yosuke Ando, Masakazu Hatano, Takenao Koseki, Shigeki Yamada","doi":"10.21873/invivo.14087","DOIUrl":"10.21873/invivo.14087","url":null,"abstract":"<p><strong>Background/aim: </strong>We previously reported that benzodiazepines with anticholinergic effects are a risk factor for infection in patients with diffuse large B-cell lymphoma; however, the effects of other anticholinergic drugs and the trend of severe infections, such as febrile neutropenia (FN), have not been investigated. The Japanese Anticholinergic Risk Scale (JARS) was developed by the Japanese Society of Geriatric Pharmacy to screen for potentially inappropriate medications with anticholinergic effects. This study aimed to elucidate the trend of FN induced by anticancer chemotherapy in patients who received anticholinergic drugs listed in the JARS.</p><p><strong>Patients and methods: </strong>We obtained data from the JADER. Reporting odds ratios (RORs) were used to detect safety signals for FN. Anticholinergic drugs were classified into three categories according to their JARS score. Safety signals for FN were considered positive if the lower limit of the 95% confidence interval (CI) of the ROR was >1.</p><p><strong>Results: </strong>A total of 162,918 cases were included in the dataset. RORs for FN indicated that cases with an anticholinergic risk score of 1 and 3 showed a safety signal (score 1: ROR=1.748, 95%CI=1.659-1.841; score 3: ROR=1.525, 95%CI=1.371-1.696), while no safety signals were observed for those with a risk score of 2 (ROR=1.015, 95%CI=0.863-1.194). Similar trends were observed in cases 70 years old and over and those under 70 years old.</p><p><strong>Conclusion: </strong>The trend of FN in patients who received an anticholinergic drug listed in the JARS was similar among younger and older patients.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 5","pages":"2872-2882"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Lenvatinib Therapy for Thyroid Cancer by Pathological Type: A Retrospective Real-world Study.","authors":"Mioko Matsuo, Mamoru Ito, Kenji Tsuchihashi, Hirofumi Ohmura, Kazuki Hashimoto, Ryunosuke Kogo, Masanobu Sato, Shogo Masuda, Takashi Nakagawa","doi":"10.21873/invivo.14085","DOIUrl":"https://doi.org/10.21873/invivo.14085","url":null,"abstract":"<p><strong>Background/aim: </strong>Refractory differentiated thyroid carcinoma (DTC) and anaplastic thyroid carcinoma (ATC) are associated with poor prognoses. Molecularly targeted agents such as lenvatinib are expected to significantly improve outcomes. However, data from real-world settings remain limited.</p><p><strong>Patients and methods: </strong>A retrospective analysis was conducted in 48 patients with thyroid carcinoma treated with lenvatinib at Kyushu University Hospital between April 2015 and October 2024. We compared overall survival (from time of treatment initiation to death, censoring, or data cutoff) and progression-free survival (from time of treatment initiation to tumor progression as confirmed by image examination) using Kaplan-Meier analysis. A subgroup analysis was also conducted for patients who had and had not received prior radioactive iodine (RAI) therapy.</p><p><strong>Results: </strong>The analysis included 29 female and 19 male cases. The histologic types were papillary thyroid carcinoma in 26 patients, follicular thyroid carcinoma in seven patients, and ATC in 15 patients. Of 33 DTC cases, RAI was not administered in nine cases. The median progression-free survival was 30 months for papillary thyroid carcinoma, 18 months for follicular thyroid carcinoma, and four months for ATC. The median progression-free survival for patients with DTC who received RAI therapy was 19 months, whereas that for patients with DTC without RAI therapy was 46 months. No significant difference was found between the two groups (<i>p</i>=0.243).</p><p><strong>Conclusion: </strong>Lenvatinib may be effective in patients with DTC in whom RAI treatment is not feasible in real-world settings.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 5","pages":"2854-2861"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GS-441524 Treatment in a Cat With Feline Infectious Peritonitis Virus and Pyogranulomatous Transverse Colon Lesion.","authors":"Tae-Young Kim, Ye-In Oh","doi":"10.21873/invivo.14074","DOIUrl":"https://doi.org/10.21873/invivo.14074","url":null,"abstract":"<p><strong>Background/aim: </strong>Feline infectious peritonitis (FIP) is a fatal disease caused by feline coronavirus (FCoV), manifesting as effusive (wet) or non-effusive (dry) forms. Granulomatous lesions in the gastrointestinal tract, particularly the colon, are rare and pose diagnostic and therapeutic challenges.</p><p><strong>Case report: </strong>A 7-year-old castrated male domestic shorthair cat (4.1 kg) presented with anorexia and vomiting. Blood tests showed an albumin:globulin ratio of 0.5, a mild elevation in aspartate aminotransferase, mild leukocytosis, and a severe elevation of feline serum amyloid A. The abdominal ultrasound and computed tomography imaging showed a mass in the transverse colon and surrounding mesenteric lymph nodes. The mass was observed to be relatively well vascularized, with areas of low-density uneven necrosis. Histological examination revealed severe pyogranulomatous inflammation with macrophages, neutrophils, and lymphocytes predominantly present in both the affected colon and lymphoid tissues. Immunohistochemistry for feline infectious peritonitis (FIP) virus antigen of the lesion showed a strong positive result, confirming limited and localized lesion induction by FIP virus infection. The cat received GS-441524 for 12 weeks, resulting in clinical improvement, lesion resolution, and normalized lab results. RT-PCR and antibody tests were negative post-treatment.</p><p><strong>Conclusion: </strong>This case report describes a rare presentation of FIP virus infection, characterized by localized lesions confined to a specific segment of the colon. In this case, treatment with the nucleoside analog GS-441524 was very effective in improving the localized lesion and demonstrated excellent efficacy in clearing the FIP virus.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 5","pages":"2760-2765"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracheal Reconstruction Using a Nitinol Stent for Thoracic Tracheal Rupture.","authors":"Min-Ho Park, Joong-Hyun Kim, Seok Hwa Choi","doi":"10.21873/invivo.14063","DOIUrl":"https://doi.org/10.21873/invivo.14063","url":null,"abstract":"<p><strong>Background/aim: </strong>Endotracheal tube intubation is required for surgery under inhalation anesthesia. In the present study, after inhalation anesthesia in dogs, the tracheal tube was extubated without deflating the endotracheal tube cuff, resulting in thoracic tracheal rupture, subcutaneous emphysema, and pneumomediastinum. The ruptured thoracic trachea was reconstructed using an endotracheal stent to treat intrathoracic emphysema.</p><p><strong>Materials and methods: </strong>A double-wire woven nitinol stent was handmade using a cross-and-hook knitting method. The fabricated endotracheal stent was 2-3 mm larger than the internal diameter of the ruptured trachea. The clinical signs and respiratory pattern, image diagnoses (radiography and computed tomography), and tracheoscopy results after endotracheal stenting were assessed for six months.</p><p><strong>Results: </strong>The lateral cervical radiographs showed that the intratracheal stent was properly placed without migration or stent fracture at the insertion site. After two to three weeks of tracheal stenting, the coughing and dyspnea signs revolved, and the normal activities in all dogs were resumed.</p><p><strong>Conclusion: </strong>The double-wire braided nitinol stent showed no migration or deformation in the canine trachea. These results suggest that the nitinol stent is compatible with the canine tracheal structure and has flexibility with an adequate radial force.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 5","pages":"2629-2633"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396041/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activin A/Follistatin Axis in Airway Diseases and its Association With Recurrent Exacerbations.","authors":"Argyro Chatziantoniou, Konstantinos Karagiannis, Alexander G Mathioudakis, Eliza Tsitoura, Eugenia Synolaki, Eirini Apostolou, Paschalis Sideras, Nikolaos Tzanakis, Nikolaos M Siafakas, Katerina M Antoniou","doi":"10.21873/invivo.14058","DOIUrl":"https://doi.org/10.21873/invivo.14058","url":null,"abstract":"<p><strong>Background/aim: </strong>Chronic airway diseases, including chronic obstructive pulmonary disease (COPD), asthma, and asthma COPD overlap (ACO), are characterized by complex inflammatory processes in which Activin A-a key member of the TGF-β superfamily-is implicated. Although its role in the stable state of these diseases has been extensively studied, data regarding its involvement during exacerbations remain limited. Our objective was to investigate the dynamics of Activin A in sputum and serum during acute exacerbations and subsequent convalescence in patients with chronic airway diseases.</p><p><strong>Patients and methods: </strong>In this prospective study, 53 patients with asthma, COPD, and ACO, aged 14 years and older were recruited upon hospitalization for an acute exacerbation. Sputum and peripheral blood samples were collected at admission, hospital discharge, one month, and four months post-exacerbation. Protein levels of Activin A were quantified by ELISA were compared among the patient groups and the kinetics of Activin A expression following exacerbation events were examined.</p><p><strong>Results: </strong>Baseline Activin A levels were comparable across the patient groups. However, during exacerbations, sputum Activin A levels increased markedly and subsequently declined during convalescence, while serum levels exhibited an inverse pattern-being lower during exacerbations and rising during stable periods. Notably, COPD patients with recurrent exacerbations maintained persistently elevated sputum Activin A levels throughout the study period, suggesting that sustained local inflammation may predispose these patients to frequent exacerbations.</p><p><strong>Conclusion: </strong>These findings reveal a compartment-specific regulation of Activin A in chronic airway diseases, underscoring its potential as a biomarker for disease activity and exacerbation risk.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 5","pages":"2573-2583"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Frailty and Use of Potentially Inappropriate Medications in Elderly Hospitalized Patients: A Cross-Sectional Study.","authors":"Yukako Morisaki, Ayako Maeda-Minami, Masaki Sato, Sayaka Izumi, Masanori Suzuki, Ryohkan Funakoshi, Yasunari Mano","doi":"10.21873/invivo.14099","DOIUrl":"https://doi.org/10.21873/invivo.14099","url":null,"abstract":"<p><strong>Background/aim: </strong>In a super-aging society, understanding frailty and potentially inappropriate medication (PIM) use is crucial for appropriate drug use in older persons. This study evaluated the association between frailty and PIMs in elderly hospitalized patients.</p><p><strong>Patients and methods: </strong>Patients ≥65 years who were admitted to Kameda Medical Center between October 2016 and 2017 were included. Frailty was defined as a Barthel Index <90 or Mini-Mental State Examination <18; patients not meeting these criteria were classified as non-frail. PIMs were defined according to the 2015 Beers Criteria. When PIMs were used at least once during hospitalization, patients were defined as PIM users; otherwise, they were defined as non-users. PIM users in both the frail and non-frail groups were compared through logistic regression analysis by adjusting for background factors and calculating adjusted odds ratios and 95% confidence intervals. As a sensitivity analysis, the frail group was subdivided into those with mild frailty and those with severe frailty, and the proportion of PIM users with mild frailty, severe frailty, and those without frailty were compared.</p><p><strong>Results: </strong>The proportion of PIM users did not significantly differ between the groups with and without frailty (adjusted odds ratio=1.29, 95% confidence interval=0.84-1.98). As a result of sensitivity analysis, the proportion of PIM use was significantly higher in the group with severe frailty than in the non-frail group (adjusted odds ratio=12.0, 95% confidence interval=1.76-81.3).</p><p><strong>Conclusion: </strong>This study found no significant difference in PIM use between frail and non-frail elderly Japanese inpatients. However, sensitivity analysis revealed higher PIM use in those with severe frailty than in those who were non-frail. Therefore, this result implies that patients with severe frailty must be prescribed drugs appropriately, taking into account the background of each patient.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 5","pages":"2986-2992"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}