In vivo最新文献

{"title":"Conversion Surgery Performed Following Durvalumab Combined With Gemcitabine and Cisplatin in Cholangiocarcinoma: A Case Report.","authors":"Yoshiyuki Tagayasu, Rin Yamada, Kosuke Kanemitsu, Yoshihiko Kondo, Rumi Itoyama, Hiromitsu Hayashi, Yoshihiro Komohara, Masaaki Iwatsuki","doi":"10.21873/invivo.13974","DOIUrl":"https://doi.org/10.21873/invivo.13974","url":null,"abstract":"<p><strong>Background/aim: </strong>Immunotherapy using immune checkpoint inhibitors (ICIs) has been widely approved for many cancers. ICI therapy has also been performed for unresectable bile duct cancer in recent years. However, there are few reports of conversion surgery following ICI therapy for unresectable or borderline resectable bile duct cancer. Herein, we present a case of conversion surgery following immune checkpoint ICI therapy for unresectable cholangiocarcinoma, focusing on the cancer immune microenvironment of this case.</p><p><strong>Case report: </strong>A 77-year-old man was diagnosed with borderline resectable, distal bile duct cancer and hilar cholangiocarcinoma. The patient underwent four courses of durvalumab combined with gemcitabine and cisplatin (Dur+GC) therapy. Evaluation of disease progression showed stable disease (SD), and considering the patient's surgical risk, a pancreaticoduodenectomy was performed. Adenocarcinoma components remained, and detailed pathological examinations using immunohistochemistry were performed. Marked infiltration of lymphocytes was observed in both the cancer core area and the margin area. The lymphocytes were positive for CD3 and CD8, with a subset also expressing CD103. PD-L1 expression was weakly positive in the stromal area, and positive cells were likely to be infiltrating macrophages in morphological features. Cancer cells were positive for HLA-A/B/C and beta-2.</p><p><strong>Conclusion: </strong>CD103+ CD8+ T cells, recently referred to as tissue-resident memory T cells, might be a critical immune cell population involved in ICI-induced anticancer immune responses in cholangiocarcinoma.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1724-1730"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CT-based Anatomic and Clinical Analysis of Iliac Screw Placement During Spinopelvic Fixation.","authors":"Carina Fritzsche, Samy Mahjoub, Tobias Hüfner, Stephan Sehmisch, Sebastian Decker","doi":"10.21873/invivo.13951","DOIUrl":"https://doi.org/10.21873/invivo.13951","url":null,"abstract":"<p><strong>Background/aim: </strong>Iliac screws provide strong caudal anchorage for both long spinal fusions as well as short lumbopelvic fixations. However, anatomic based placement can be challenging, and complication rates are often underestimated.</p><p><strong>Patients and methods: </strong>We analysed 47 iliac screws being placed in 24 patients. Using postoperative computed tomography (CT), iliac screw placement was analysed with reference to anatomic landmarks. Iliac narrowings were described with regard to their relevance for iliac screw placement. Moreover, we analyzed clinical records for clinical complications. The latter were classified as intraoperative, postoperative, and radiological.</p><p><strong>Results: </strong>From starting points, described by distance to the posterior superior iliac spine (PSIS), the average iliac screw length was 71.2±13.7 mm, and the diameter was as wide as 7.9±0.7 mm. Divergence was 30.7±12.6° (transverse plane) and caudal orientation was 34.2±13.0° (sagittal orientation). General pelvic dimensions correlated significantly with each other, and certainly with the length of implanted screws. Different adverse events and complications occurred. A total of 20% of patients were found with at least partial extracortical malpositioning. The main group of complications were postoperative with painful prominence in 20% of cases, wound infection in 8.9% and wound healing disorders in 6.7%. Further complications were radiological screw loosening (11.1%). No complications were detected in 33.3% of patients.</p><p><strong>Conclusion: </strong>Optimal iliac screw size relative to the individual anatomy in general is not achieved. In most cases compared to the literature, iliac screw dimensions could be both longer and thicker. Perfect anatomic placement can be challenging, which highlights the need for individual preoperative CT-based surgical planning to achieve a strong caudal anchorage in lumbopelvic fixations. In general, the diameter seems to be more important than the screw length.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1514-1523"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144020172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors for Discontinuation of Naldemedine Therapy in a Palliative Ward.","authors":"Takaki Kanie, Tomohiro Mizuno, Takenao Koseki, Aya Hanamoto, Hiroko Sawano, Masako Tomida, Yukiko Kakumae, Takahiro Hayashi, Hiroshi Matsuoka, Masanobu Usui, Shigeki Yamada","doi":"10.21873/invivo.13965","DOIUrl":"https://doi.org/10.21873/invivo.13965","url":null,"abstract":"<p><strong>Background/aim: </strong>Opioid-induced constipation (OIC) is a common adverse drug event in patients undergoing chronic pain therapy. Naldemedine is an oral, peripherally acting μ-opioid receptor antagonist that improves bowel movement without affecting opioid pain relief. In palliative wards, many patients experience malnutrition caused by cachexia and systemic inflammation because of cancer progression. We investigated whether the C-reactive protein-to-albumin ratio (CAR) affects the continuation of naldemedine therapy in a palliative ward.</p><p><strong>Patients and methods: </strong>We included Japanese patients in the palliative ward of Fujita Health University Hospital between April 2020 and August 2023 in this retrospective observational study. The log-rank test was used to compare the continuation rates of naldemedine over 14 days. Cox proportional hazards analysis was performed using the terms morphine-equivalent daily dose <30 mg and CAR ≥0.888.</p><p><strong>Results: </strong>Eighty patients were divided into continuation (n=58) and discontinuation (n=22) groups. The proportion of patients with a CAR ≥0.888 was significantly higher in the discontinuation group than in the continuation group (<i>p</i> =0.020). Cox proportional hazards analysis showed that morphine-equivalent daily dose <30 mg was not a factor for discontinuation of naldemedine therapy (hazard ratio=1.040, <i>p</i>=0.929) but CAR ≥0.888 was (hazard ratio=3.251, <i>p</i>=0.035).</p><p><strong>Conclusion: </strong>A high CAR (≥0.888) was a risk factor for the discontinuation of naldemedine therapy in a palliative ward. Our results suggest that physicians and pharmacists should monitor CAR as a marker of malnutrition and systemic inflammation before initiating naldemedine therapy.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1647-1653"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surgical Treatment and Prognosis of Soft Tissue Sarcoma in Patients Aged 85 Years and Older.","authors":"Hiroyuki Tsuchie, Makoto Emori, Shohei Murata, Yasutaka Murahashi, Emi Mizushima, Junya Shimizu, Hiroyuki Nagasawa, Atsushi Teramoto, Naohisa Miyakoshi","doi":"10.21873/invivo.13967","DOIUrl":"https://doi.org/10.21873/invivo.13967","url":null,"abstract":"<p><strong>Background/aim: </strong>The incidence of soft tissue sarcomas (STSs) among older patients is increasing. Although it has been reported that surgical treatment of elderly patients with STS improves prognosis, most of these studies included patients aged <85 years. This study aimed to analyze the clinical features and prognostic factors of STS in excessively elderly patients aged ≥85 or ≥90 years.</p><p><strong>Patients and methods: </strong>We retrospectively identified 398 patients with STS who were treated at our two hospitals between 1994 and 2022. Only histological types that existed in ≥20 cases were included. Data on clinical information and detailed assessments were collected. We divided the patients into two subgroups according to 85 or 90 years of age and compared the groups within each subgroup. Furthermore, we examined the factors affecting the prognosis of all and older patients.</p><p><strong>Results: </strong>Patients ≥85 or ≥90 years old comprised 12.1% and 4.0% of the study population, respectively. In all patients, Kaplan-Meier curves for overall survival demonstrated a significantly poorer prognosis in elderly patients with STS aged ≥85 years (<i>p</i>=0.0476) and ≥90 years (<i>p</i>=0.0164). However, no significant differences were observed when analyzing only patients who underwent surgical treatment without distant metastasis at diagnosis. In the multivariate analyses, surgical treatment for the primary tumor improved prognosis exclusively in patients ≥85 years old (<i>p</i> =0.0300).</p><p><strong>Conclusion: </strong>Surgical treatment improves the prognosis in elderly STS patients aged ≥85 years and possibly among those aged ≥90 years.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1661-1668"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041974/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of BDNF in the Cortex and Hippocampus of Mice With Middle Cerebral Artery Occlusion.","authors":"Hyo-In Hwang, Yoon Young Chung, Hye-Kyoung Shin, Dong-Joon Kim, Yong Hyun Jun","doi":"10.21873/invivo.13943","DOIUrl":"https://doi.org/10.21873/invivo.13943","url":null,"abstract":"<p><strong>Background/aim: </strong>Stroke is a cerebrovascular disease with high mortality and disability, causing motor dysfunction and cognitive impairments. Middle cerebral artery occlusion (MCAO) mouse models of ischemic stroke are used for identifying therapeutic targets. Ischemic insults to the brain alter brain-derived neurotrophic factor (BDNF) expression in cortical and hippocampal neurons. In the present study, we investigated BDNF expression in the cortex and hippocampus of mice following MCAO.</p><p><strong>Materials and methods: </strong>Monofilament sutures coated with silicone rubber were introduced into the common carotid artery and occluded middle cerebral artery. The filament was withdrawn for reperfusion after 0.5 h. BDNF protein expression was measured using western blot. Immunofluorescence was performed with anti-NeuN and anti-BDNF antibodies.</p><p><strong>Results: </strong>BDNF expression in the cerebral cortex and hippocampus was decreased one and three days after MCAO, compared to the control group (unoperated mice). BDNF was expressed in NeuN-positive neurons in the dentate gyrus of the hippocampus and motor cortex of the MCAO and control groups.</p><p><strong>Conclusion: </strong>MCAO in mice reduced the expression of BDNF in mature neurons of both the motor cortex and hippocampus at one and three days after surgery.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1414-1421"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Connexin-43 Protein Expression Pattern Analysis in Myocardial Infarction Tissues.","authors":"Alexandros Tsantoulas, Dimitrios Roukas, Sofianiki Mastronikoli, Evangelos Tsiambas, George Tsouvelas, Nikolaos Kafkas, Panagiotis Fotiades, Sotirios Papouliakos, George Agrogiannis, Andreas C Lazaris, Nikolaos Kavantzas","doi":"10.21873/invivo.13940","DOIUrl":"https://doi.org/10.21873/invivo.13940","url":null,"abstract":"<p><strong>Background/aim: </strong>Ischemic heart disease is a leading cause of death worldwide in comparison to malignant neoplasia. Myocardial infarction (MI) is the result of severe ischemia due to a low consumption of oxygen in the myocardium. The main pathophysiological reason is a progressive obstructive atherosclerotic endothelial lesion that causes reduction in coronary blood flow and increases the corresponding arterial stenosis. Our research aim was to investigate the role of altered expression connexin-43 (gene locus: 6q22.31) protein in MI tissue substrates with different clinico-pathological characteristics.</p><p><strong>Materials and methods: </strong>A set of fifty (n=50) MI archival tissue sections derived from a forensic pathology file were selected and micro-sectioned. Immunohistochemistry and digital image analysis assays were implemented for detecting and measuring the levels of connexin-43, respectively.</p><p><strong>Results: </strong>Low expression of Connexin-43 protein was detected in 16/50 (32%) cases, biphasic expression pattern (low/medium) was identified in 10/50 (20%), whereas moderate and high levels of protein expression were observed in the rest of them (24/50-48%). Connexin-43 overall expression was significantly correlated with the timing of the MI onset (recent or past) (<i>p</i>=0.001).</p><p><strong>Conclusion: </strong>Connexin-43 is a critical gap junction intermediate protein in MI pathology diagnosis and research. Different Connexin-43 expression levels, including single phase or biphasic patterns, should be a reliable biomarker for determining the timing of the MI lesions inside the corresponding tissue sections. Furthermore, implementation of sophisticated, accurate computerized techniques, such as digital image analysis provide very detailed, objective results regarding protein expression as modern precise (evidence-based) medicine requires.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1370-1377"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143984757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microstructural, Electrochemical, and Mechanical Assessment of Additive Manufactured Titanium Grade 23 for Dental Implants Application.","authors":"Mirko Teschke, Lorenz Grafe, Sebastian Stammkötter, Jochen Tenkamp, Frank Walther","doi":"10.21873/invivo.13978","DOIUrl":"https://doi.org/10.21873/invivo.13978","url":null,"abstract":"<p><strong>Background/aim: </strong>Due to its excellent mechanical properties and biocompatibility, the titanium grade 23 alloy is the material of choice for dental implants. Additive manufacturing enables patient-specific manufacturing and the reduction of stress shielding by using lattice structures instead of solid material. For the simulation and design of such structures, a comprehensive knowledge of the mechanical properties under quasi-static and cyclic loading, the microstructure, and the electrochemical properties is required. In addition, suitable heat treatments must be selected and validated. These properties were determined uniformly and will provide a complete database and benchmark for future applications.</p><p><strong>Materials and methods: </strong>The mechanical behavior of the laser powder bed fusion (PBF-LB/M) manufactured alloy Ti6Al4V in the as-built and heat-treated state was characterized in tensile and constant amplitude tests, as well as hardness and microstructure analysis. To characterize the electrochemical properties, electrochemical impedance spectroscopy and potentiodynamic polarization measurements were performed.</p><p><strong>Results: </strong>For use in medical implants, both conditions fulfilled the mechanical required specification in DIN EN ISO 5832-3, but heat treatment also reduced the high residual stresses caused by the manufacturing process. In the high cycle fatigue range, no significant difference was found between the two material states. The fatigue strength was increased compared to the literature. In electrochemical corrosion investigations, no remarkable differences between the two material states were detected by electrochemical impedance spectroscopy or potentiodynamic polarization measurements, but the high corrosion resistance of PBF-LB/M Ti6Al4V was demonstrated.</p><p><strong>Conclusion: </strong>The study provides a wide electrochemical and mechanical database for the design of the PBF-LB/M manufactured alloy Titanium grade 21 as an implant material.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1751-1766"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"End-to-end Anastomosis With ECHELON ENDOPATH™ Staple Line Reinforcement for Right-sided Colon Cancer: Safety and Feasibility.","authors":"Yasuhiro Ishiyama, Yasumitsu Hirano, Megumi Sasaki, Sohei Akuta, Masatoshi Yoshizawa, Misuzu Yamato, Akihito Nakanishi, Yume Minagawa, Hisashi Hayashi, Takatsugu Fujii, Naoto Okazaki, Toshimasa Ishii, Chikashi Hiranuma","doi":"10.21873/invivo.13957","DOIUrl":"https://doi.org/10.21873/invivo.13957","url":null,"abstract":"<p><strong>Background/aim: </strong>Functional end-to-end anastomosis (FEEA) is widely utilized in right-sided colorectal cancer surgery for joining the colon and ileum using mechanical staplers. ECHELON ENDOPATH™ Staple Line Reinforcement (SLR), a synthetic absorbable material introduced in 2020, represents a novel approach to reinforcing the anastomosis. However, surgical outcomes of FEEA utilizing SLR remain unreported in the literature. This retrospective study aimed to evaluate the efficacy and safety of FEEA with SLR compared to conventional FEEA in elective right-sided colon cancer surgery.</p><p><strong>Patients and methods: </strong>We conducted a retrospective analysis of 159 consecutive patients who underwent elective surgery for right-sided colon cancer at our institution between February 2019 and December 2022. Short-term surgical outcomes were compared between cohorts receiving FEEA with and without SLR.</p><p><strong>Results: </strong>Analysis revealed no significant differences between groups in operative time, blood loss, postoperative hospital stay duration, or overall complication rates. The SLR group demonstrated a significantly higher proportion of robotic surgical procedures (<i>p</i><0.001). Intraoperative staple line intervention was less frequently required in the SLR group (<i>p</i>=0.012). While one case of postoperative anastomotic leakage was documented in the non-SLR group, no such complications were observed in the SLR group.</p><p><strong>Conclusion: </strong>SLR represents a safe and effective adjunctive technique for FEEA in right-sided colon cancer surgery. Further validation through larger prospective studies is warranted to confirm these preliminary results.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1573-1579"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Over-expression of Truncated IK Ameliorates Dinitrochlorobenzene-Induced Allergic Contact Dermatitis Lesions in BALB/c Mice.","authors":"Jehee Son, Eun Young Oh, Sohyun Park, Sang-Myeong Lee","doi":"10.21873/invivo.13941","DOIUrl":"https://doi.org/10.21873/invivo.13941","url":null,"abstract":"<p><strong>Background/aim: </strong>Allergic contact dermatitis (ACD) is a delayed-type hypersensitivity reaction mediated by hapten-specific T cells. Dinitrochlorobenzene (DNCB)-induced mouse models are widely used to investigate the pathogenesis of contact dermatitis. Inhibitor K562 (IK) cytokine suppresses IFN-γ-induced MHC class II expression on B cells by increasing cAMP levels. Previously, we reported that truncated IK (tIK) expression in transgenic (Tg) mice ameliorated rheumatoid arthritis by suppressing CD4⁺ T helper cells (Th)-1 and Th17 cell differentiation, as well as macrophage activation. However, its role in hypersensitivity diseases such as ACD remains underexplored. This study aimed to evaluate whether tIK Tg mice exhibit reduced susceptibility to DNCB-induced ACD and passive systemic anaphylaxis (PSA).</p><p><strong>Materials and methods: </strong>ACD was induced in BALB/c and tIK Tg mice through repeated DNCB application. Ear thickness and scratching behavior were assessed. Serum IgE levels and mast cell-associated gene expression were analyzed. Th cell differentiation was evaluated using flow cytometry. PSA, an experimental model used to study systemic allergic reactions, was induced by IgE sensitization followed by antigen challenge, and hypothermia, serum IgE, and mast cell activation were measured.</p><p><strong>Results: </strong>DNCB-treated BALB/c mice developed severe dermatitis, including increased ear thickness and scratching behavior, whereas tIK Tg mice exhibited milder symptoms. tIK over-expression also led to lower serum IgE levels and reduced mast cell-associated gene expression. T cell analysis revealed suppressed Th2 and Th17 differentiation, while Tregs and Th1 cells remained unaffected. Beyond ACD, tIK Tg mice exhibited attenuated PSA responses, with less severe hypothermia, lower serum IgE levels, and reduced mast cell activation compared to wild-type controls.</p><p><strong>Conclusion: </strong>tIK suppresses both localized and systemic hypersensitivity by modulating Th cell differentiation and mast cell activity. tIK may serve as a potential therapeutic target for allergic and inflammatory diseases.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1378-1393"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143994530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transjugular Liver Biopsy for Histological Diagnosis of Refractory Ascites and Evaluation of Portal Hypertension.","authors":"Toru Ishikawa, Ryo Sato, Hiroki Natsui, Takahiro Iwasawa, Masahiro Ogawa, Yuji Kobayashi, Toshifumi Sato, Junji Yokoyama, Terasu Honma","doi":"10.21873/invivo.13959","DOIUrl":"https://doi.org/10.21873/invivo.13959","url":null,"abstract":"<p><strong>Background/aim: </strong>Determining the etiology of underlying liver disease is important when deciding on the optimal treatment course for refractory hepatic ascites. However, percutaneous liver biopsy is not recommended for such patients. To address this issue, we investigated the safety profile and diagnostic efficacy of wedged hepatic venous pressure (WHVP) measurement and transjugular liver biopsy (TJLB) to facilitate the histological diagnosis and evaluation of portal hypertension in cases of refractory ascites.</p><p><strong>Patients and methods: </strong>The clinical background, laboratory diagnostic success rate, complication rate, and survival-related factors were analyzed in 24 patients with refractory ascites, extracted from among 314 patients who underwent TJLB between May 2002 and December 2024.</p><p><strong>Results: </strong>Overall, 15/9 cases were male/female, respectively, the mean age, Alb level, MELD score, and WHVP were 67.21 years, 2.61 g/dl, 24.917, and 38.24 cmH2O, respectively. The overall diagnostic success rate of the TJLB procedure was 100.0%, providing sufficient material for diagnosis. No severe complications were observed. Cases of ascites caused by chronic myelomonocytic leukemia (n=1) and amyloidosis (n=4) had a poor prognosis, whereas cases caused by autoimmune hepatitis (AIH) had a good prognosis following disease treatment. AIH and Child-Pugh class B were identified as factors contributing to survival.</p><p><strong>Conclusion: </strong>TJLB has the advantage of allowing the assessment of portal venous pressure with WHVP, facilitating the histological diagnosis in cases of refractory ascites. TJLB could thus represent an alternative to percutaneous liver biopsy in patients with ascites, possibly contributing to better prognosis by allowing treatment strategy decisions according to the underlying disease cause.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1591-1597"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}