In vivo最新文献

{"title":"\"Gerontosurgery\": Evaluation of Multidimensional Assessment Scales for Elderly Patients Undergoing Major Abdominal Surgery. What Is the Best Prediction Model?","authors":"Marta Goglia, Diana Ronconi, Andrea DE Zanna, Arianna Cicolani, Gaetano Gallo, Niccolò Petrucciani, Matteo Pavone, Francesco D'Angelo, Giuseppe Nigri, Paolo Aurello","doi":"10.21873/invivo.14040","DOIUrl":"10.21873/invivo.14040","url":null,"abstract":"<p><strong>Background/aim: </strong>The aging population poses unique challenges in oncogeriatric surgery, particularly regarding risk stratification and postoperative outcome prediction. The impact of frailty on surgical decision-making reports should be noted. Geriatric assessment scales are recommended for preoperative objective evaluations to optimize surgical outcomes, but their accuracy remains unclear. This study evaluates the effectiveness of five multidimensional geriatric assessment scales - Charlson Comorbidity Index (CCI), APACHE II, Cumulative Illness Rating Scale (CIRS), Identification of Seniors at Risk (ISAR), and G8 - in predicting postoperative complications in elderly patients undergoing major oncological surgery.</p><p><strong>Patients and methods: </strong>A retrospective observational study was conducted on 69 patients aged ≥75 years who underwent major abdominal surgery for neoplastic conditions between December 2018 and July 2020. Preoperative assessments using the five scoring systems were performed, and postoperative complications were classified according to the Clavien-Dindo system. The study compared the predictive validity of each scale through correlation analysis with postoperative outcomes.</p><p><strong>Results: </strong>The study found weak correlations between predictive scores and postoperative complications. The G8 scale showed the strongest association with Clavien-Dindo scores (<i>ρ</i>=0.267, <i>p</i>=0.027), while other scales exhibited limited predictive value. Despite the widespread use of these scales in clinical practice, none of them accurately predicted postoperative morbidity in this cohort of patients.</p><p><strong>Conclusion: </strong>This study highlights the limitations of existing geriatric assessment scales in predicting postoperative complications for elderly patients undergoing major oncological surgery. Among the evaluated tools, only the G8 score showed an association with complications. However, the results suggest a need for more tailored risk stratification models that incorporate comorbidities, frailty, nutritional status, and physiological reserves. Further research with larger sample sizes is necessary to validate these findings and improve preoperative decision-making in geriatric oncologic surgery.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 4","pages":"2410-2418"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prostate-specific Antigen Decline During Primary Androgen-deprivation Therapy for Predicting Response and Survival in Metastatic Castration-resistant Prostate Cancer Patients Receiving Enzalutamide.","authors":"Yujiro Nagata, Takaomi Sugi, Sohei Yamamura, Yoshihiro Sugita, Yui Mizushima, Takuo Matsukawa, Tomohisa Takaba, Kazumasa Jojima, Katsuyoshi Higashijima, Masahiro Matsumoto, Akinori Minato, Ikko Tomisaki, Eiji Kashiwagi, Naohiro Fujimoto","doi":"10.21873/invivo.14016","DOIUrl":"10.21873/invivo.14016","url":null,"abstract":"<p><strong>Background/aim: </strong>Currently, there are no established predictive or prognostic biomarkers for first-line enzalutamide (ENZ) treatment in patients with metastatic castration-resistant prostate cancer (mCRPC). This multicenter study aimed to assess the predictive and prognostic significance of the initial-to-nadir prostate-specific antigen (PSA) ratio (I/N PSA) during primary androgen-deprivation therapy for <i>de novo</i> metastatic castration-sensitive prostate cancer in relation to the response to first-line ENZ in mCRPC.</p><p><strong>Patients and methods: </strong>A total of 33 patients with mCRPC receiving first-line enzalutamide were included in the study to investigate the correlation between I/N PSA in combined androgen blockade and clinical outcomes. Patients were dichotomized by median I/N PSA values. A PSA response was defined as a 90% or greater decline in PSA levels following the initiation of ENZ treatment in patients with mCRPC.</p><p><strong>Results: </strong>The median I/N PSA was 382. In the high I/N PSA (≥382) group, the PSA response rate was 75.0%, significantly higher than that in the low I/N PSA group (35.3%; <i>p</i>=0.037). The median overall survival following ENZ treatment was significantly better in the high I/N PSA group than in the low group (<i>p</i>≤0.01). Multivariable analysis demonstrated I/N PSA as an independent predictor of overall survival (hazard ratio=0.20; <i>p</i>≤0.01).</p><p><strong>Conclusion: </strong>In patients with mCRPC, the I/N PSA is a promising predictive and prognostic biomarker for first-line ENZ treatment and may provide personalized approaches in daily practice.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 4","pages":"2209-2218"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IgG4-related Disease of the Retroperitoneum Mimics Malignancy: A Case Report and Literature Review.","authors":"Gaia Cicioni, Immacolata Iannone, Daniele Crocetti, Cristina DE Padua, Alessandro Coppola, Luigi Petramala, Paolo Sapienza, Claudio Letizia","doi":"10.21873/invivo.14011","DOIUrl":"10.21873/invivo.14011","url":null,"abstract":"<p><strong>Background/aim: </strong>Immunoglobulin G4-related disease (IgG4-RD) is a systemic, immune-mediated condition characterized by fibroinflammatory lesions affecting multiple organs. When localized in the retroperitoneum, it may mimic malignancy and often leads to surgical intervention. We report the case of a 21-year-old male with retroperitoneal IgG4-RD and review the relevant literature, emphasizing the diagnostic challenges and importance of differential diagnosis.</p><p><strong>Patients and methods: </strong>A case report of a 21-year-old man with retroperitoneal IgG4-RD is presented. A literature review was conducted <i>via</i> Scopus, Embase, and Medline through December 2024, selecting studies with immunohistochemically confirmed retroperitoneal IgG4-RD. Data were independently extracted and analyzed using SPSS software.</p><p><strong>Results: </strong>Of 74 articles retrieved, 22 met the inclusion criteria, totaling 23 patients including our case. The mean age was 62 years, with a male predominance (65%). The left ureter was most commonly involved (52%). Frequent symptoms included localized abdominal pain (61%) and hydronephrosis (96%). Imaging often suggested malignancy, leading 74% of patients to undergo surgery. Histopathology confirmed IgG4-RD in all cases, though only 30% showed IgG4 immunohistochemical positivity.</p><p><strong>Conclusion: </strong>Retroperitoneal IgG4-RD closely mimics malignancy, posing significant diagnostic challenges. Elevated serum IgG4 levels and tissue biopsy are critical for accurate diagnosis. IgG4-RD should be considered in patients with unexplained retroperitoneal masses to avoid unnecessary surgery and ensure appropriate treatment.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 4","pages":"2154-2164"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Case of Seronegative Lupus Nephritis Detected Incidentally by Renal Biopsy.","authors":"Akira Mima, Takamasa Matsuki, Takahiro Nakamoto, Yuta Saito, Takaaki Morikawa, Sakura Kure, Keishi Matsumoto, Shinji Lee","doi":"10.21873/invivo.14029","DOIUrl":"10.21873/invivo.14029","url":null,"abstract":"<p><strong>Background/aim: </strong>Systemic lupus erythematosus (SLE) and lupus nephritis (LN) are autoimmune diseases that affect multiple organ systems with the production of pathological autoantibodies.</p><p><strong>Case report: </strong>We describe the case of a 19-year-old man who had microscopic hematuria and mild proteinuria. No skin rash, joint symptoms, serositis, neuropathy, or cytopenia were observed. Renal biopsy revealed the thickness of the glomerular basement membrane, granular deposits of C1q along mesangial areas, and high electron density deposits in basement membrane on the epithelial side of the glomerulus. After operating renal biopsy, laboratory tests were performed and the results were not associated with SLE. This leads to the diagnosis of class V seronegative LN. The patient achieved remission only with angiotensin II receptor blocker without immunosuppressive drugs. There have been few reports of seronegative LN.</p><p><strong>Conclusion: </strong>Our case report presents a case of seronegative LN identified incidentally during renal biopsy.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 4","pages":"2328-2332"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"JADER Database-based Analysis of the Association Between Tranexamic Acid and Thromboembolism and Related Risk Factors.","authors":"Natsuko Ishida, Toshihiro Shibata, Yukio Suga, Junko Ishizaki","doi":"10.21873/invivo.14025","DOIUrl":"https://doi.org/10.21873/invivo.14025","url":null,"abstract":"<p><strong>Background/aim: </strong>Tranexamic acid (TXA) is widely used as a hemostatic agent and for various other indications, including dermatological and inflammatory conditions. However, its association with thromboembolism in the real-world setting remains unclear. In this study, we aimed to evaluate this association.</p><p><strong>Patients and methods: </strong>We collected data from the Japan Adverse Drug Event Report database from Q1 2004 to Q3 2020 and performed reporting odds ratio analyses and multivariate logistic regression analysis.</p><p><strong>Results: </strong>Overall, 1,735,923 reports were analyzed. TXA was the suspected causal drug in 54 cases of venous thromboembolism (VTE) and 8 cases of arterial thromboembolism. A significant signal was detected for VTE (reporting odds ratio=6.61; 95% confidence interval=5.03-8.70) but not for arterial thromboembolism. VTE cases involving TXA were more frequently reported in women and individuals with a body mass index of ≥25 than in other groups.</p><p><strong>Conclusion: </strong>TXA may be associated with VTE. The findings highlight the importance of careful patient monitoring during TXA administration, particularly in high-risk populations such as women and individuals who are overweight.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 4","pages":"2295-2301"},"PeriodicalIF":1.8,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conversion Surgery Performed Following Durvalumab Combined With Gemcitabine and Cisplatin in Cholangiocarcinoma: A Case Report.","authors":"Yoshiyuki Tagayasu, Rin Yamada, Kosuke Kanemitsu, Yoshihiko Kondo, Rumi Itoyama, Hiromitsu Hayashi, Yoshihiro Komohara, Masaaki Iwatsuki","doi":"10.21873/invivo.13974","DOIUrl":"https://doi.org/10.21873/invivo.13974","url":null,"abstract":"<p><strong>Background/aim: </strong>Immunotherapy using immune checkpoint inhibitors (ICIs) has been widely approved for many cancers. ICI therapy has also been performed for unresectable bile duct cancer in recent years. However, there are few reports of conversion surgery following ICI therapy for unresectable or borderline resectable bile duct cancer. Herein, we present a case of conversion surgery following immune checkpoint ICI therapy for unresectable cholangiocarcinoma, focusing on the cancer immune microenvironment of this case.</p><p><strong>Case report: </strong>A 77-year-old man was diagnosed with borderline resectable, distal bile duct cancer and hilar cholangiocarcinoma. The patient underwent four courses of durvalumab combined with gemcitabine and cisplatin (Dur+GC) therapy. Evaluation of disease progression showed stable disease (SD), and considering the patient's surgical risk, a pancreaticoduodenectomy was performed. Adenocarcinoma components remained, and detailed pathological examinations using immunohistochemistry were performed. Marked infiltration of lymphocytes was observed in both the cancer core area and the margin area. The lymphocytes were positive for CD3 and CD8, with a subset also expressing CD103. PD-L1 expression was weakly positive in the stromal area, and positive cells were likely to be infiltrating macrophages in morphological features. Cancer cells were positive for HLA-A/B/C and beta-2.</p><p><strong>Conclusion: </strong>CD103+ CD8+ T cells, recently referred to as tissue-resident memory T cells, might be a critical immune cell population involved in ICI-induced anticancer immune responses in cholangiocarcinoma.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1724-1730"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CT-based Anatomic and Clinical Analysis of Iliac Screw Placement During Spinopelvic Fixation.","authors":"Carina Fritzsche, Samy Mahjoub, Tobias Hüfner, Stephan Sehmisch, Sebastian Decker","doi":"10.21873/invivo.13951","DOIUrl":"https://doi.org/10.21873/invivo.13951","url":null,"abstract":"<p><strong>Background/aim: </strong>Iliac screws provide strong caudal anchorage for both long spinal fusions as well as short lumbopelvic fixations. However, anatomic based placement can be challenging, and complication rates are often underestimated.</p><p><strong>Patients and methods: </strong>We analysed 47 iliac screws being placed in 24 patients. Using postoperative computed tomography (CT), iliac screw placement was analysed with reference to anatomic landmarks. Iliac narrowings were described with regard to their relevance for iliac screw placement. Moreover, we analyzed clinical records for clinical complications. The latter were classified as intraoperative, postoperative, and radiological.</p><p><strong>Results: </strong>From starting points, described by distance to the posterior superior iliac spine (PSIS), the average iliac screw length was 71.2±13.7 mm, and the diameter was as wide as 7.9±0.7 mm. Divergence was 30.7±12.6° (transverse plane) and caudal orientation was 34.2±13.0° (sagittal orientation). General pelvic dimensions correlated significantly with each other, and certainly with the length of implanted screws. Different adverse events and complications occurred. A total of 20% of patients were found with at least partial extracortical malpositioning. The main group of complications were postoperative with painful prominence in 20% of cases, wound infection in 8.9% and wound healing disorders in 6.7%. Further complications were radiological screw loosening (11.1%). No complications were detected in 33.3% of patients.</p><p><strong>Conclusion: </strong>Optimal iliac screw size relative to the individual anatomy in general is not achieved. In most cases compared to the literature, iliac screw dimensions could be both longer and thicker. Perfect anatomic placement can be challenging, which highlights the need for individual preoperative CT-based surgical planning to achieve a strong caudal anchorage in lumbopelvic fixations. In general, the diameter seems to be more important than the screw length.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1514-1523"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144020172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors for Discontinuation of Naldemedine Therapy in a Palliative Ward.","authors":"Takaki Kanie, Tomohiro Mizuno, Takenao Koseki, Aya Hanamoto, Hiroko Sawano, Masako Tomida, Yukiko Kakumae, Takahiro Hayashi, Hiroshi Matsuoka, Masanobu Usui, Shigeki Yamada","doi":"10.21873/invivo.13965","DOIUrl":"https://doi.org/10.21873/invivo.13965","url":null,"abstract":"<p><strong>Background/aim: </strong>Opioid-induced constipation (OIC) is a common adverse drug event in patients undergoing chronic pain therapy. Naldemedine is an oral, peripherally acting μ-opioid receptor antagonist that improves bowel movement without affecting opioid pain relief. In palliative wards, many patients experience malnutrition caused by cachexia and systemic inflammation because of cancer progression. We investigated whether the C-reactive protein-to-albumin ratio (CAR) affects the continuation of naldemedine therapy in a palliative ward.</p><p><strong>Patients and methods: </strong>We included Japanese patients in the palliative ward of Fujita Health University Hospital between April 2020 and August 2023 in this retrospective observational study. The log-rank test was used to compare the continuation rates of naldemedine over 14 days. Cox proportional hazards analysis was performed using the terms morphine-equivalent daily dose <30 mg and CAR ≥0.888.</p><p><strong>Results: </strong>Eighty patients were divided into continuation (n=58) and discontinuation (n=22) groups. The proportion of patients with a CAR ≥0.888 was significantly higher in the discontinuation group than in the continuation group (<i>p</i> =0.020). Cox proportional hazards analysis showed that morphine-equivalent daily dose <30 mg was not a factor for discontinuation of naldemedine therapy (hazard ratio=1.040, <i>p</i>=0.929) but CAR ≥0.888 was (hazard ratio=3.251, <i>p</i>=0.035).</p><p><strong>Conclusion: </strong>A high CAR (≥0.888) was a risk factor for the discontinuation of naldemedine therapy in a palliative ward. Our results suggest that physicians and pharmacists should monitor CAR as a marker of malnutrition and systemic inflammation before initiating naldemedine therapy.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1647-1653"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surgical Treatment and Prognosis of Soft Tissue Sarcoma in Patients Aged 85 Years and Older.","authors":"Hiroyuki Tsuchie, Makoto Emori, Shohei Murata, Yasutaka Murahashi, Emi Mizushima, Junya Shimizu, Hiroyuki Nagasawa, Atsushi Teramoto, Naohisa Miyakoshi","doi":"10.21873/invivo.13967","DOIUrl":"https://doi.org/10.21873/invivo.13967","url":null,"abstract":"<p><strong>Background/aim: </strong>The incidence of soft tissue sarcomas (STSs) among older patients is increasing. Although it has been reported that surgical treatment of elderly patients with STS improves prognosis, most of these studies included patients aged <85 years. This study aimed to analyze the clinical features and prognostic factors of STS in excessively elderly patients aged ≥85 or ≥90 years.</p><p><strong>Patients and methods: </strong>We retrospectively identified 398 patients with STS who were treated at our two hospitals between 1994 and 2022. Only histological types that existed in ≥20 cases were included. Data on clinical information and detailed assessments were collected. We divided the patients into two subgroups according to 85 or 90 years of age and compared the groups within each subgroup. Furthermore, we examined the factors affecting the prognosis of all and older patients.</p><p><strong>Results: </strong>Patients ≥85 or ≥90 years old comprised 12.1% and 4.0% of the study population, respectively. In all patients, Kaplan-Meier curves for overall survival demonstrated a significantly poorer prognosis in elderly patients with STS aged ≥85 years (<i>p</i>=0.0476) and ≥90 years (<i>p</i>=0.0164). However, no significant differences were observed when analyzing only patients who underwent surgical treatment without distant metastasis at diagnosis. In the multivariate analyses, surgical treatment for the primary tumor improved prognosis exclusively in patients ≥85 years old (<i>p</i> =0.0300).</p><p><strong>Conclusion: </strong>Surgical treatment improves the prognosis in elderly STS patients aged ≥85 years and possibly among those aged ≥90 years.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1661-1668"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041974/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of BDNF in the Cortex and Hippocampus of Mice With Middle Cerebral Artery Occlusion.","authors":"Hyo-In Hwang, Yoon Young Chung, Hye-Kyoung Shin, Dong-Joon Kim, Yong Hyun Jun","doi":"10.21873/invivo.13943","DOIUrl":"https://doi.org/10.21873/invivo.13943","url":null,"abstract":"<p><strong>Background/aim: </strong>Stroke is a cerebrovascular disease with high mortality and disability, causing motor dysfunction and cognitive impairments. Middle cerebral artery occlusion (MCAO) mouse models of ischemic stroke are used for identifying therapeutic targets. Ischemic insults to the brain alter brain-derived neurotrophic factor (BDNF) expression in cortical and hippocampal neurons. In the present study, we investigated BDNF expression in the cortex and hippocampus of mice following MCAO.</p><p><strong>Materials and methods: </strong>Monofilament sutures coated with silicone rubber were introduced into the common carotid artery and occluded middle cerebral artery. The filament was withdrawn for reperfusion after 0.5 h. BDNF protein expression was measured using western blot. Immunofluorescence was performed with anti-NeuN and anti-BDNF antibodies.</p><p><strong>Results: </strong>BDNF expression in the cerebral cortex and hippocampus was decreased one and three days after MCAO, compared to the control group (unoperated mice). BDNF was expressed in NeuN-positive neurons in the dentate gyrus of the hippocampus and motor cortex of the MCAO and control groups.</p><p><strong>Conclusion: </strong>MCAO in mice reduced the expression of BDNF in mature neurons of both the motor cortex and hippocampus at one and three days after surgery.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1414-1421"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}