In vivo最新文献

{"title":"Neutropenic Enterocolitis in Acute Myeloid Leukemia and Morbus Behcet: Pivotal Role of Medical Imaging and Multidisciplinary Management in a Complex Clinical Case.","authors":"Christian A Dascalescu, Alessia Fraccaroli, Erik Gaitzsch, Samixa Pudasaini, Felix L Herr, Jens Ricke, Clemens C Cyran, Karsten Spiekermann, Michael VON Bergwelt-Baildon, Joachim Stemmler, Max Seidensticker, Wolfgang G Kunz","doi":"10.21873/invivo.14102","DOIUrl":"https://doi.org/10.21873/invivo.14102","url":null,"abstract":"<p><strong>Background/aim: </strong>Neutropenic enterocolitis (NE), also known as typhlitis, is a life-threatening gastrointestinal complication primarily affecting immunocompromised patients undergoing intensive chemotherapy. Its management becomes particularly challenging when compounded by comorbidities such as Behçet's disease with gastrointestinal involvement.</p><p><strong>Case report: </strong>We report the case of a 44-year-old male with acute myeloid leukemia (AML) and intestinal Behçet's disease who developed severe NE during induction chemotherapy. Initial conservative management was guided by serial cross-sectional imaging, which revealed progressive bowel wall thickening, pneumatosis intestinalis, and signs of ileus. The clinical course was complicated by septic shock, multiorgan dysfunction, and later, acute gastrointestinal hemorrhage. Active arterial bleeding was identified via computed tomography angiography and successfully treated with interventional radiological coil embolization. Surgical intervention was avoided. Multidisciplinary collaboration, including hematology, radiology, rheumatology, intensive care, and pain management, was essential in navigating this complex clinical scenario.</p><p><strong>Conclusion: </strong>This case highlights the pivotal role of radiological imaging in diagnosing and monitoring NE and demonstrates the value of interventional radiology as a minimally invasive alternative to surgery. In patients with overlapping autoimmune and oncological pathologies, individualized, multidisciplinary management is crucial for optimizing outcomes.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 5","pages":"3008-3013"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peripheral Blood-based <i>NRN1</i> and <i>TNFAIP3</i> Gene Expression as Minimally Invasive Biomarkers for Gastric Cancer.","authors":"Daniel Soares Queiroz, Yeda Beatriz Louredo Santos, Leonardo Caires Santos, Elizabeth Suchi Chen, Carolina Oliveira Gigek, Laercio Gomes Lourenço, Carlos Haruo Arasaki, Ricardo Artigiani, Nora Manoukian Forones, Marilia Arruda Cardoso Smith, Fernanda Wisnieski","doi":"10.21873/invivo.14065","DOIUrl":"https://doi.org/10.21873/invivo.14065","url":null,"abstract":"<p><strong>Background/aim: </strong>Gastric cancer (GC) remains one of the most common and fatal cancer types worldwide. Herein, we explored neuritin 1 (<i>NRN1</i>) and tumor necrosis factor alpha-induced protein 3 (<i>TNFAIP3</i>) as minimally invasive biomarkers of GC.</p><p><strong>Materials and methods: </strong>The <i>NRN1</i> and <i>TNFAIP3</i> mRNA levels in the peripheral blood samples of 42 patients with GC were compared with 12 individuals with no cancer diagnosis, and monitored after gastrectomy.</p><p><strong>Results: </strong><i>NRN1</i> expression was elevated in patients compared with controls (<i>p</i><0.01), and was able to discriminate between these groups (area under the curve=0.72, <i>p</i>=0.02). After resection of the primary tumor, <i>NRN1</i> expression decreased (<i>p</i><0.01), reaching levels observed in controls. <i>TNFAIP3</i> expression showed no difference between patients and controls, nor postoperatively.</p><p><strong>Conclusion: </strong>Peripheral blood-based <i>NRN1</i> expression can be used as a minimally invasive biomarker for GC detection and postoperative monitoring.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 5","pages":"2646-2658"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396042/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk Factors for 30-day Mortality After Emergency Surgery for Non-occlusive Mesenteric Ischemia.","authors":"Yume Minagawa, Yasuhiro Ishiyama, Manabu Amiki, Yasumitsu Hirano","doi":"10.21873/invivo.14089","DOIUrl":"https://doi.org/10.21873/invivo.14089","url":null,"abstract":"<p><strong>Background/aim: </strong>Non-occlusive mesenteric ischemia (NOMI) is a rare and highly fatal disease characterized by intestinal ischemia or necrosis despite the absence of obstruction of the mesenteric vessels. This research aimed to investigate the risk factors for 30-day mortality of NOMI after emergency surgery.</p><p><strong>Patients and methods: </strong>We analyzed 42 consecutive patients who underwent emergency surgery for NOMI from April 2015 to August 2022. Patients were divided into two groups, based on 30-day postoperative survival as follows: early death (30-day mortality group, n=9) and survival (survivor group, n=33). The characteristics, past history, and surgical outcomes were compared between the groups.</p><p><strong>Results: </strong>The 30-day mortality rate was 21.4%. There were no significant differences in the patients' characteristics and surgical complications between groups. However, the 30-day mortality group had a significantly higher number of patients with chronic kidney disease (CKD) (<i>p</i><0.01) and on hemodialysis (<i>p</i>=0.01), as well as a significantly lower platelet count (<i>p</i>=0.02). In the multivariate analysis, a preoperative lactate dehydrogenase >1,127 U/l was identified as an independent risk factor for 30-day mortality following emergency surgery for NOMI (odds ratio=23.6; 95% confidence interval=2.12-262.9; <i>p</i>=0.01).</p><p><strong>Conclusion: </strong>CKD, hemodialysis, high LDH, and low platelet count were found to be risk factors for early mortality from NOMI.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 5","pages":"2893-2897"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asbestos Exposure and Leukemia Incidence: A Systematic Review and Meta-analysis.","authors":"Konstantinos Dodos, Tsampika-Vasileia Kalamara, Petros Papalexis, Dimitrios Ap Keramydas, Effie G Papageorgiou, Vasiliki Epameinondas Georgakopoulou","doi":"10.21873/invivo.14072","DOIUrl":"https://doi.org/10.21873/invivo.14072","url":null,"abstract":"<p><strong>Background/aim: </strong>Asbestos comprises six naturally occurring fibrous minerals known for their health risks, particularly in occupational settings. This systematic review evaluates the association between asbestos exposure and leukemia incidence, synthesizing findings from various studies.</p><p><strong>Materials and methods: </strong>We conducted a comprehensive literature search in PubMed, Cochrane Library, and Web of Science, adhering to PRISMA guidelines. Studies included participants exposed to asbestos compared to matched controls, focusing on leukemia incidence. Data extraction and quality assessment were performed using the Newcastle-Ottawa Scale.</p><p><strong>Results: </strong>A total of 1,751,580 participants were included, with 257,572 (14.7%) exposed to asbestos. The incidence of leukemia varied across studies, ranging from 0.02% to 0.45%. Meta-analysis revealed an overall risk ratio of 1.25 (95% confidence interval=0.81-1.94) with significant heterogeneity (I²=86%), indicating no statistically significant difference between exposed and non-exposed cohorts.</p><p><strong>Conclusion: </strong>The findings highlight the complexity of the relationship between asbestos exposure and leukemia, influenced by factors such as exposure type, duration, and confounding variables like smoking. While some studies suggest a potential link, the evidence remains inconclusive, necessitating further research. This review underscores the need for high-quality studies to clarify the association between asbestos and leukemia, informing public health policies to reduce exposure risks and protect vulnerable populations.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 5","pages":"2739-2751"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Albumin-bilirubin (ALBI) and Platelet-ALBI (PALBI) Grades: Novel Prognostic Factors for Cholangiocellular Carcinoma.","authors":"Ergin Aydemir, Funda Yilmaz, Alper Türkel, Öztürk Ateş, Mutlu Doğan","doi":"10.21873/invivo.14098","DOIUrl":"https://doi.org/10.21873/invivo.14098","url":null,"abstract":"<p><strong>Background/aim: </strong>Cholangiocarcinoma (CCA) is the second most frequently occurring primary malignant tumor of the liver, characterized by poor survival due to late diagnosis and limited treatment options. The albumin-bilirubin (ALBI) and platelet-ALBI (PALBI) scores, which reflect liver function and inflammation, have emerged as potential prognostic markers in hepatocellular carcinoma (HCC). Their prognostic significance in CCA, however, remains less established.</p><p><strong>Patients and methods: </strong>A retrospective analysis was conducted on 184 patients diagnosed with CCA between 2007 and 2024. The study evaluated the relationship between tumor location, ALBI/PALBI grades, and overall survival (OS). Patients were categorized into three groups based on their ALBI and PALBI scores, and survival outcomes were analyzed.</p><p><strong>Results: </strong>Tumor location significantly impacted OS. The median OS (mOS) was 18 months for distal CCA, 13 months for perihilar CCA, and 7 months for intrahepatic CCA (<i>p</i><0.001). When stratified by ALBI grade, mOS was 17 months for Grade 1, 8 months for Grade 2, and 2 months for Grade 3 (<i>p</i>=0.001). Similarly, for PALBI grade, mOS was 13 months for Grade A1, 11 months for Grade A2, and 8 months for Grade A3 (<i>p</i>=0.037). Among the variables included in the multivariate analysis, only the ALBI grade retained its significance as an independent prognostic factor for overall survival.</p><p><strong>Conclusion: </strong>ALBI and PALBI grades serve as effective prognostic indicators in CCA, with lower grades associated with enhanced survival rates. Notably, ALBI grade was found to be an independent predictor of OS, presenting a cost-efficient biomarker that may support clinical decision-making by providing crucial prognostic information insights.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 5","pages":"2976-2985"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396068/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement in Coronary Dose Distribution Using Dynamic Swing Arc in Volumetric-modulated Arc Therapy During Esophageal Cancer Radiotherapy.","authors":"Kouta Hirotaki, Kenji Makita, Masashi Wakabayashi, Hidehiro Hojo, Satoe Kitou, Kento Tomizawa, Masashi Ito, Takeji Sakae","doi":"10.21873/invivo.14082","DOIUrl":"https://doi.org/10.21873/invivo.14082","url":null,"abstract":"<p><strong>Background/aim: </strong>The dose to the left anterior descending coronary artery (LAD) is associated with mortality in patients with esophageal cancer (EC) who underwent radiotherapy. The aim of this study was to compare the dose distributions to the LAD region achieved through volumetric-modulated arc therapy (VMAT) planning using a dynamic swing arc in OXRAY (DSA-VMAT) and conventional coplanar (Conv-VMAT) planning.</p><p><strong>Patients and methods: </strong>Ten patients with EC who had undergone radiotherapy (60 Gy in 30 fractions) at our Institution were selected for inclusion in the study. Two virtual plans (DSA-VMAT and Conv-VMAT) were created to compare the dose distributions of the LAD region, heart, lungs, and planning target volume (PTV). All plans were analyzed using paired <i>t</i>-tests.</p><p><strong>Results: </strong>The mean values±standard deviation for 15 Gy to the LAD region (V15) were 10.48±13.04% for DSA-VMAT and 30.28±23.56% for Conv-VMAT. Compared with Conv-VMAT, DSA-VMAT significantly improved V15 of the LAD region (<i>p</i>=0.01). In addition, DSA-VMAT significantly reduced the mean heart dose (8.64±5.37 <i>vs.</i> 11.23±7.37 Gy), heart V40 (17.55±4.76% <i>vs.</i> 20.44±6.06%), lung V20 (14.87±5.93% <i>vs.</i> 17.81±7.70%), and lung V5 (57.27±8.24% <i>vs.</i> 61.15±9.97%) compared to Conv-VMAT (all <i>p</i>≤0.01). In contrast, there were no significant differences between the two groups in PTV dose coverage [D95 (<i>p</i>=0.61), D50 (<i>p</i>=0.62)], or conformity index (<i>p</i>=0.91).</p><p><strong>Conclusion: </strong>Compared with Conv-VMAT, DSA-VMAT improved the dose distribution of the LAD region without impairing the PTV dose coverage. Thus, DSA-VMAT may reduce radiation-induced heart disease in patients with EC without loss of efficacy.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 5","pages":"2824-2831"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396035/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silymarin Ameliorates Tacrolimus-induced Inflammation in Human Umbilical Vein Endothelial Cells.","authors":"Yu-Chia Chen, Kai-Tun Chang, Po-Ming Chen, Hsin-Hung Chen","doi":"10.21873/invivo.14062","DOIUrl":"https://doi.org/10.21873/invivo.14062","url":null,"abstract":"<p><strong>Background/aim: </strong>Tacrolimus (TAC), a cornerstone immunosuppressant in solid organ transplantation, is associated with significant cardiovascular toxicities, including endothelial dysfunction and inflammation. Silymarin (SM), a natural flavonoid complex from milk thistle, possesses known antioxidant and anti-inflammatory properties. This study investigated the pro-inflammatory effects of TAC on human umbilical vein endothelial cells (HUVECs) and evaluated the potential protective capacity of SM. This study aimed to investigate the inflammatory response induced by TAC in HUVECs and to determine whether co-treatment with SM can ameliorate TAC-induced inflammation.</p><p><strong>Materials and methods: </strong>HUVECs were cultured and treated with TAC (20 μg/ml) for varying durations (6, 24, 48, 72 h) under different culture conditions (1% or 2% FBS pre-starvation) to optimize the inflammatory response model. Lipopolysaccharide (LPS) served as a positive control. The optimized condition involved pre-starvation in 2% FBS medium followed by a 6-h induction. In the key experiment, HUVECs were treated with vehicle, TAC (20 μg/ml), or TAC (20 μg/ml) plus SM (50 μg/ml) for 6 h. The expression levels of pro-inflammatory mediators interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and cyclooxygenase-2 (COX-2) were assessed using western blotting.</p><p><strong>Results: </strong>TAC (20 μg/ml) significantly induced the expression of IL-1β, TNF-α, and COX-2 in HUVECs, particularly after 6 h of induction following pre-starvation in 2% FBS medium. Co-treatment with SM (50 μg/ml) markedly suppressed the TAC-induced up-regulation of all three inflammatory markers (IL-1β, TNF-α, and COX-2) compared to treatment with TAC alone.</p><p><strong>Conclusion: </strong>TAC directly promotes an inflammatory phenotype in HUVECs. SM effectively counteracts this TAC-induced endothelial inflammation <i>in vitro</i>. These findings suggest that silymarin, potentially through its antioxidant and NF-κB inhibitory actions, could be explored as a therapeutic agent to mitigate the vascular inflammation and endothelial dysfunction associated with TAC treatment in transplant recipients, potentially reducing cardiovascular complications.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 5","pages":"2617-2628"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396071/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Superficial Angiomyxoma Revisited.","authors":"Yuki Shinohara, Yoshiro Chijiiwa, Jun Nishio","doi":"10.21873/invivo.14053","DOIUrl":"https://doi.org/10.21873/invivo.14053","url":null,"abstract":"<p><p>Superficial angiomyxoma (SAM) is a rare benign mesenchymal tumor of uncertain differentiation that primarily occurs in the trunk, lower extremities and head and neck of middle-aged adults. It typically presents as a slow-growing, painless polypoid or papulonodular lesion. Ultrasonography shows an ovoid, well-defined mass with a homogeneous, hypoechoic echotexture. Color Doppler examination may demonstrate prominent hypervascularity. Computed tomography reveals a round to oval hypodense mass. On magnetic resonance imaging, SAM usually exhibits low to intermediate signal intensity on T1-weighted sequences and high signal intensity on T2-weighted sequences. Heterogenous enhancement is seen after intravenous contrast administration. Histologically, the lesion is composed of bland spindle-shaped and stellate-shaped cells in an abundant myxoid stroma with numerous small blood vessels. By immunohistochemistry, the tumor cells are positive for CD34 and show variable staining for smooth muscle actin and S-100 protein. Loss of protein kinase cAMP-dependent type I regulatory subunit alpha (PRKAR1A) expression is observed in a significant subset of cases. Complete surgical excision is the treatment of choice for SAM. This review provides an updated overview of the clinicopathological, radiological and genomic features of SAM and discusses the differential diagnosis of this peculiar tumor.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 5","pages":"2505-2511"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial Damage-induced Ferroptosis: The Molecular Mechanism by Which Psoralen Inhibits the Proliferation and Invasion of Non-small-cell Lung Cancer Cells.","authors":"Hangyu Deng, Jincheng Tang, Yun Xu, Ling Wu, Jingting Zhang, Hongyao Chen, Zhibin Wang, Renyi Yang, Wenhui Gao, Zuomei He","doi":"10.21873/invivo.14068","DOIUrl":"https://doi.org/10.21873/invivo.14068","url":null,"abstract":"<p><strong>Background/aim: </strong>Ferroptosis, an iron-dependent form of cell death mediated by lipid peroxidation, plays a critical role in non-small-cell lung cancer (NSCLC) progression. Psoralen, a bioactive natural compound, exhibits anticancer properties, but its effects and mechanisms in NSCLC remain unclear. This study explored whether psoralen induces ferroptosis by triggering mitochondrial damage and investigates the underlying molecular mechanisms.</p><p><strong>Materials and methods: </strong>Cell Counting Kit-8 was used to assess the impact of psoralen on cell viability, while 5-ethynyl-2'-deoxyuridine incorporation, colony-formation, scratch wound-healing, and Transwell assays evaluated its effects on proliferation, migration, and invasion. FerroOrange and 2',7'-dichlorodihydrofluorescein diacetate fluorescence probes, Western blot, and kits for malondialdehyde (MDA), lipid peroxidation (LPO), reduced glutathione (GSH), and oxidized glutathione disulfide (GSSG) were used to assess ferroptosis-related markers. JC-1, MitoTracker Green, and MitoSOX Red probes, along with transmission electron microscopy, were used to evaluate mitochondrial damage. Bioinformatics analysis, network pharmacology, and molecular docking were conducted to elucidate potential mechanisms.</p><p><strong>Results: </strong>Psoralen disrupted mitochondrial structure and function; increased Fe²⁺ accumulation; elevated levels of reactive oxygen species, MDA and LPO; depleted GSH; and downregulated glutathione peroxidase 4 (<i>GPX4</i>) and solute carrier family 7 member 11 (<i>SLC7A11</i>), ultimately inducing ferroptosis and inhibiting NSCLC cell proliferation and invasion. Eleven key target genes (<i>PRKCB, MIF, GPI, AKR1C3, PDE3B, VDR, ALOX5, PTGS2, NQO1, MMP13, and CA9</i>) were identified, with enrichment analysis linking them to arachidonic acid metabolism, vascular endothelial growth factor signaling, lipid metabolism, and oxidative stress. Molecular docking confirmed strong binding affinity of psoralen' to these targets.</p><p><strong>Conclusion: </strong>Psoralen induces ferroptosis in NSCLC by disrupting mitochondrial structure and function. These findings highlight its potential as a natural ferroptosis-targeting agent and provide insights for developing psoralen-based anticancer therapeutics.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 5","pages":"2681-2702"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Worsening Nutritional Status in Neoadjuvant Chemotherapy on Postoperative Outcomes in Esophageal Cancer.","authors":"Makoto Hasegawa, Zenichiro Saze, Hiroki Yago, Hiroya Suzuki, Dai Mitsui, Akira Matsuishi, Yuya Maruyama, Hajime Matsuida, Tomohiro Kikuchi, Azuma Nirei, Hiroyuki Hanayama, Koji Kono","doi":"10.21873/invivo.14084","DOIUrl":"https://doi.org/10.21873/invivo.14084","url":null,"abstract":"<p><strong>Background/aim: </strong>Esophageal squamous cell carcinoma (ESCC) significantly affects nutritional status. While neoadjuvant chemotherapy (NAC) is the standard treatment for clinical stage II/III ESCC, its impact on nutritional status and postoperative outcomes remains unclear. This study investigated the relationship between nutritional deterioration during NAC and outcomes following esophagectomy.</p><p><strong>Patients and methods: </strong>This single-center retrospective study included 85 patients with thoracic ESCC who received NAC followed by esophagectomy between January 2019 and December 2023. The NAC regimens included cisplatin plus 5-fluorouracil (CF), administered with or without radiation, and docetaxel, cisplatin, and fluorouracil (DCF). Nutritional status and postoperative outcomes were evaluated.</p><p><strong>Results: </strong>Of the 85 patients, 20 received DCF, 36 received CF alone, and 29 received CF with radiation. Nutritional deterioration was noted during NAC with significant decreases in Prognostic Nutritional Index (PNI), Geriatric Nutritional Risk Index (GNRI), and hemoglobin-albumin-lymphocyte-platelet (HALP) score. Grade 3-4 hematologic toxicities correlated with reductions in GNRI and increases in neutrophil-lymphocyte ratio (NLR) (<i>p</i>=0.004 and 0.020, respectively). An increase in NLR post-NAC and decrease in PNI post-NAC were associated with prolonged hospital stays (<i>p</i>=0.024 and 0.042, respectively). Post-NAC NLR of 2.3 or over and HALP score below 20 were significantly associated with poorer overall survival (<i>p</i>=0.012 and <0.001, respectively).</p><p><strong>Conclusion: </strong>Although NAC reduces tumor burden and eliminates micrometastases, it potentially worsens nutritional status. Chemotherapy-induced hematologic toxicities are a risk factor for this decline. Therefore, comprehensive nutritional assessment and timely intervention during NAC are essential to optimize patient outcomes.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 5","pages":"2842-2853"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12396034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}