In vivo最新文献

{"title":"Connexin-43 Protein Expression Pattern Analysis in Myocardial Infarction Tissues.","authors":"Alexandros Tsantoulas, Dimitrios Roukas, Sofianiki Mastronikoli, Evangelos Tsiambas, George Tsouvelas, Nikolaos Kafkas, Panagiotis Fotiades, Sotirios Papouliakos, George Agrogiannis, Andreas C Lazaris, Nikolaos Kavantzas","doi":"10.21873/invivo.13940","DOIUrl":"https://doi.org/10.21873/invivo.13940","url":null,"abstract":"<p><strong>Background/aim: </strong>Ischemic heart disease is a leading cause of death worldwide in comparison to malignant neoplasia. Myocardial infarction (MI) is the result of severe ischemia due to a low consumption of oxygen in the myocardium. The main pathophysiological reason is a progressive obstructive atherosclerotic endothelial lesion that causes reduction in coronary blood flow and increases the corresponding arterial stenosis. Our research aim was to investigate the role of altered expression connexin-43 (gene locus: 6q22.31) protein in MI tissue substrates with different clinico-pathological characteristics.</p><p><strong>Materials and methods: </strong>A set of fifty (n=50) MI archival tissue sections derived from a forensic pathology file were selected and micro-sectioned. Immunohistochemistry and digital image analysis assays were implemented for detecting and measuring the levels of connexin-43, respectively.</p><p><strong>Results: </strong>Low expression of Connexin-43 protein was detected in 16/50 (32%) cases, biphasic expression pattern (low/medium) was identified in 10/50 (20%), whereas moderate and high levels of protein expression were observed in the rest of them (24/50-48%). Connexin-43 overall expression was significantly correlated with the timing of the MI onset (recent or past) (<i>p</i>=0.001).</p><p><strong>Conclusion: </strong>Connexin-43 is a critical gap junction intermediate protein in MI pathology diagnosis and research. Different Connexin-43 expression levels, including single phase or biphasic patterns, should be a reliable biomarker for determining the timing of the MI lesions inside the corresponding tissue sections. Furthermore, implementation of sophisticated, accurate computerized techniques, such as digital image analysis provide very detailed, objective results regarding protein expression as modern precise (evidence-based) medicine requires.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1370-1377"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143984757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microstructural, Electrochemical, and Mechanical Assessment of Additive Manufactured Titanium Grade 23 for Dental Implants Application.","authors":"Mirko Teschke, Lorenz Grafe, Sebastian Stammkötter, Jochen Tenkamp, Frank Walther","doi":"10.21873/invivo.13978","DOIUrl":"https://doi.org/10.21873/invivo.13978","url":null,"abstract":"<p><strong>Background/aim: </strong>Due to its excellent mechanical properties and biocompatibility, the titanium grade 23 alloy is the material of choice for dental implants. Additive manufacturing enables patient-specific manufacturing and the reduction of stress shielding by using lattice structures instead of solid material. For the simulation and design of such structures, a comprehensive knowledge of the mechanical properties under quasi-static and cyclic loading, the microstructure, and the electrochemical properties is required. In addition, suitable heat treatments must be selected and validated. These properties were determined uniformly and will provide a complete database and benchmark for future applications.</p><p><strong>Materials and methods: </strong>The mechanical behavior of the laser powder bed fusion (PBF-LB/M) manufactured alloy Ti6Al4V in the as-built and heat-treated state was characterized in tensile and constant amplitude tests, as well as hardness and microstructure analysis. To characterize the electrochemical properties, electrochemical impedance spectroscopy and potentiodynamic polarization measurements were performed.</p><p><strong>Results: </strong>For use in medical implants, both conditions fulfilled the mechanical required specification in DIN EN ISO 5832-3, but heat treatment also reduced the high residual stresses caused by the manufacturing process. In the high cycle fatigue range, no significant difference was found between the two material states. The fatigue strength was increased compared to the literature. In electrochemical corrosion investigations, no remarkable differences between the two material states were detected by electrochemical impedance spectroscopy or potentiodynamic polarization measurements, but the high corrosion resistance of PBF-LB/M Ti6Al4V was demonstrated.</p><p><strong>Conclusion: </strong>The study provides a wide electrochemical and mechanical database for the design of the PBF-LB/M manufactured alloy Titanium grade 21 as an implant material.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1751-1766"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"End-to-end Anastomosis With ECHELON ENDOPATH™ Staple Line Reinforcement for Right-sided Colon Cancer: Safety and Feasibility.","authors":"Yasuhiro Ishiyama, Yasumitsu Hirano, Megumi Sasaki, Sohei Akuta, Masatoshi Yoshizawa, Misuzu Yamato, Akihito Nakanishi, Yume Minagawa, Hisashi Hayashi, Takatsugu Fujii, Naoto Okazaki, Toshimasa Ishii, Chikashi Hiranuma","doi":"10.21873/invivo.13957","DOIUrl":"https://doi.org/10.21873/invivo.13957","url":null,"abstract":"<p><strong>Background/aim: </strong>Functional end-to-end anastomosis (FEEA) is widely utilized in right-sided colorectal cancer surgery for joining the colon and ileum using mechanical staplers. ECHELON ENDOPATH™ Staple Line Reinforcement (SLR), a synthetic absorbable material introduced in 2020, represents a novel approach to reinforcing the anastomosis. However, surgical outcomes of FEEA utilizing SLR remain unreported in the literature. This retrospective study aimed to evaluate the efficacy and safety of FEEA with SLR compared to conventional FEEA in elective right-sided colon cancer surgery.</p><p><strong>Patients and methods: </strong>We conducted a retrospective analysis of 159 consecutive patients who underwent elective surgery for right-sided colon cancer at our institution between February 2019 and December 2022. Short-term surgical outcomes were compared between cohorts receiving FEEA with and without SLR.</p><p><strong>Results: </strong>Analysis revealed no significant differences between groups in operative time, blood loss, postoperative hospital stay duration, or overall complication rates. The SLR group demonstrated a significantly higher proportion of robotic surgical procedures (<i>p</i><0.001). Intraoperative staple line intervention was less frequently required in the SLR group (<i>p</i>=0.012). While one case of postoperative anastomotic leakage was documented in the non-SLR group, no such complications were observed in the SLR group.</p><p><strong>Conclusion: </strong>SLR represents a safe and effective adjunctive technique for FEEA in right-sided colon cancer surgery. Further validation through larger prospective studies is warranted to confirm these preliminary results.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1573-1579"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Over-expression of Truncated IK Ameliorates Dinitrochlorobenzene-Induced Allergic Contact Dermatitis Lesions in BALB/c Mice.","authors":"Jehee Son, Eun Young Oh, Sohyun Park, Sang-Myeong Lee","doi":"10.21873/invivo.13941","DOIUrl":"https://doi.org/10.21873/invivo.13941","url":null,"abstract":"<p><strong>Background/aim: </strong>Allergic contact dermatitis (ACD) is a delayed-type hypersensitivity reaction mediated by hapten-specific T cells. Dinitrochlorobenzene (DNCB)-induced mouse models are widely used to investigate the pathogenesis of contact dermatitis. Inhibitor K562 (IK) cytokine suppresses IFN-γ-induced MHC class II expression on B cells by increasing cAMP levels. Previously, we reported that truncated IK (tIK) expression in transgenic (Tg) mice ameliorated rheumatoid arthritis by suppressing CD4⁺ T helper cells (Th)-1 and Th17 cell differentiation, as well as macrophage activation. However, its role in hypersensitivity diseases such as ACD remains underexplored. This study aimed to evaluate whether tIK Tg mice exhibit reduced susceptibility to DNCB-induced ACD and passive systemic anaphylaxis (PSA).</p><p><strong>Materials and methods: </strong>ACD was induced in BALB/c and tIK Tg mice through repeated DNCB application. Ear thickness and scratching behavior were assessed. Serum IgE levels and mast cell-associated gene expression were analyzed. Th cell differentiation was evaluated using flow cytometry. PSA, an experimental model used to study systemic allergic reactions, was induced by IgE sensitization followed by antigen challenge, and hypothermia, serum IgE, and mast cell activation were measured.</p><p><strong>Results: </strong>DNCB-treated BALB/c mice developed severe dermatitis, including increased ear thickness and scratching behavior, whereas tIK Tg mice exhibited milder symptoms. tIK over-expression also led to lower serum IgE levels and reduced mast cell-associated gene expression. T cell analysis revealed suppressed Th2 and Th17 differentiation, while Tregs and Th1 cells remained unaffected. Beyond ACD, tIK Tg mice exhibited attenuated PSA responses, with less severe hypothermia, lower serum IgE levels, and reduced mast cell activation compared to wild-type controls.</p><p><strong>Conclusion: </strong>tIK suppresses both localized and systemic hypersensitivity by modulating Th cell differentiation and mast cell activity. tIK may serve as a potential therapeutic target for allergic and inflammatory diseases.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1378-1393"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143994530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transjugular Liver Biopsy for Histological Diagnosis of Refractory Ascites and Evaluation of Portal Hypertension.","authors":"Toru Ishikawa, Ryo Sato, Hiroki Natsui, Takahiro Iwasawa, Masahiro Ogawa, Yuji Kobayashi, Toshifumi Sato, Junji Yokoyama, Terasu Honma","doi":"10.21873/invivo.13959","DOIUrl":"https://doi.org/10.21873/invivo.13959","url":null,"abstract":"<p><strong>Background/aim: </strong>Determining the etiology of underlying liver disease is important when deciding on the optimal treatment course for refractory hepatic ascites. However, percutaneous liver biopsy is not recommended for such patients. To address this issue, we investigated the safety profile and diagnostic efficacy of wedged hepatic venous pressure (WHVP) measurement and transjugular liver biopsy (TJLB) to facilitate the histological diagnosis and evaluation of portal hypertension in cases of refractory ascites.</p><p><strong>Patients and methods: </strong>The clinical background, laboratory diagnostic success rate, complication rate, and survival-related factors were analyzed in 24 patients with refractory ascites, extracted from among 314 patients who underwent TJLB between May 2002 and December 2024.</p><p><strong>Results: </strong>Overall, 15/9 cases were male/female, respectively, the mean age, Alb level, MELD score, and WHVP were 67.21 years, 2.61 g/dl, 24.917, and 38.24 cmH2O, respectively. The overall diagnostic success rate of the TJLB procedure was 100.0%, providing sufficient material for diagnosis. No severe complications were observed. Cases of ascites caused by chronic myelomonocytic leukemia (n=1) and amyloidosis (n=4) had a poor prognosis, whereas cases caused by autoimmune hepatitis (AIH) had a good prognosis following disease treatment. AIH and Child-Pugh class B were identified as factors contributing to survival.</p><p><strong>Conclusion: </strong>TJLB has the advantage of allowing the assessment of portal venous pressure with WHVP, facilitating the histological diagnosis in cases of refractory ascites. TJLB could thus represent an alternative to percutaneous liver biopsy in patients with ascites, possibly contributing to better prognosis by allowing treatment strategy decisions according to the underlying disease cause.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1591-1597"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of the Chick Chorioallantoic Membrane (CAM) Model for Biocompatibility Analysis of Biomaterials in the Context of the 3R-cascade: A Pilot Study.","authors":"Mike Barbeck, Kerstin Huebner, Kim Burkhardt, Tonya Andreeva, Rumen Krastev, Reiner Schnettler, Sanja Stojanovic, Stevo Najman, Ole Jung, Regine Schneider-Stock","doi":"10.21873/invivo.13977","DOIUrl":"https://doi.org/10.21873/invivo.13977","url":null,"abstract":"<p><strong>Background/aim: </strong>The present study aimed to compare the tissue responses to biomaterials in the chick chorioallantoic membrane (CAM) model with those from the subcutaneous implantation model in rats at an early time point. It was especially investigated whether histopathological scoring according to DIN EN ISO 10993-6 is also possible after biomaterial implantation using the CAM model and to what extent the values differ from the data obtained from small animal experiments.</p><p><strong>Materials and methods: </strong>Implantation of a xenogeneic bone substitute using the CAM model for 24 h and subcutaneous implantation model in rats up to 10 days post implantation were conducted. Standardized histological and histopathological methods were used to apply for histopathological scoring according to DIN EN ISO 10993-6.</p><p><strong>Results: </strong>The histological analysis as well as the histo-pathological scoring revealed that the tissue responses to the xenogeneic bone substitute were completely comparable in both organisms with no visible or statistical differences.</p><p><strong>Conclusion: </strong>We suggest that bioincompatible biomaterials can already be sorted out in the context of THE preclinical <i>in vivo</i> test phase. Such pre-testing before the required small animal tests might clearly contribute to the 3R-concept to reduce the number of animals (REDUCE).</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1740-1750"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041968/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spot Scanning Proton Beam Therapy for Surgery-inaccessible Hepatocellular Carcinoma: Preliminary Results.","authors":"Yutaka Fujimoto, Sachika Shiraishi, Masashi Yamanaka, Akihiro Yamano, Kazuki Matsumoto, Shintaro Shiba, Toshitaka Tsukiyama, Masahiro Kobayashi, Koichi Tokuuye","doi":"10.21873/invivo.13966","DOIUrl":"https://doi.org/10.21873/invivo.13966","url":null,"abstract":"<p><strong>Background/aim: </strong>Spot scanning proton beam therapy (SS-PBT) was employed for the treatment of surgery-inaccessible hepatocellular carcinoma, primarily influenced by respiratory movement.</p><p><strong>Patients and methods: </strong>Between October 2022 and December 2023, 12 patients were enrolled in this study to evaluate the efficacy of SS-PBT. The median follow-up time was 13.1 months.</p><p><strong>Results: </strong>The one-year survival, progression-free survival, and local control rates were 79.5%, 57.1%, and 100%, respectively, without grade 2 or higher PBT-related toxicities. The clinical outcomes of these 12 patients appear comparable to data from prospective studies conducted at proton centers across Japan.</p><p><strong>Conclusion: </strong>SS-PBT shows promise as a treatment option for surgery-inaccessible hepatocellular carcinoma.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1654-1660"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effectiveness of Live Birth Rate of Traditional Chinese Medicine Intervention for Infertile Women Undergoing a Second Round of IVF Is Influenced by Age.","authors":"Shu-Chiu Wang, Liang-Miin Tsai, Po-Ming Chen, Yuan-Man Hsu, Shu-Chen Wang, Chiung-Hung Chang","doi":"10.21873/invivo.13963","DOIUrl":"https://doi.org/10.21873/invivo.13963","url":null,"abstract":"<p><strong>Background/aim: </strong>While <i>in vitro</i> fertilization (IVF) is an effective treatment for infertility, the live birth rate remains suboptimal. This study aimed to evaluate the factors influencing live birth rates in infertile women undergoing a second round of IVF combined with traditional Chinese medicine (TCM) treatment.</p><p><strong>Patients and methods: </strong>This was a single-center, retrospective cohort study involving 45 infertile women undergoing a second round of IVF who underwent a combined treatment approach integrating TCM with IVF. Clinical factors affecting live birth rates were analyzed using Student's <i>t</i> test, Pearson's Chi-square test, and univariate logistic regression models.</p><p><strong>Results: </strong>The findings revealed a clinical pregnancy rate of 51.11% and a live birth rate of 42.22%, underscoring the potential effectiveness of combining TCM with a second round of IVF for infertile women. Additionally, maternal age showed a statistically significant influence on the clinical pregnancy rate (39.32±2.93 years <i>vs.</i> 36.48±2.47 years, <i>p</i>=0.005) and live birth rate (39.68±2.85 years <i>vs.</i> 35.58±2.95 years, <i>p</i><0.001).</p><p><strong>Conclusion: </strong>Integrating traditional Chinese medicine into IVF protocols notably enhances maternal success rates. For women over 38, age-specific strategies such as fertility preservation, optimized ovarian stimulation, advanced embryo selection, and oocyte donation are key to addressing age-related challenges.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1625-1637"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age- and Periodontal Disease Independent Correlation of Salivary Amino Acids.","authors":"Shoji Tanaka, Hiroshi Sakagami, Masahiro Sugimoto","doi":"10.21873/invivo.13928","DOIUrl":"https://doi.org/10.21873/invivo.13928","url":null,"abstract":"<p><strong>Background/aim: </strong>This study explored the relationship between salivary metabolomic profiles, periodontal diseases, and age.</p><p><strong>Patients and methods: </strong>Resting whole saliva samples were collected from a cohort comprising 21 women and 30 men aged 20 to 70 years, including healthy volunteers and patients with different stages of periodontal diseases. Hydrophilic metabolites were analyzed using capillary electrophoresis-mass spectrometry. The concentrations were quantified and analyzed using multivariable analysis with or without normalization to eliminate overall differences in salivary concentrations across the samples.</p><p><strong>Results: </strong>Metabolomic analysis quantified the absolute concentration of 248 metabolites in saliva samples. The unnormalized metabolomic profiles formed large clusters, with more than half of the detected metabolites showing positive correlations with each other. The absence of such clusters in the normalized data suggests the presence of individual differences in the processed data. The presence of urea, whose concentration increased gradually with the degree of progression of periodontal disease, and leucine, whose concentration decreased gradually, was identified. Highly positive correlations were observed between proline and glycine, which remained consistent regardless of normalization, age, or disease progression.</p><p><strong>Conclusion: </strong>The metabolomic profiles of salivary samples revealed unique correlations between amino acids that were independent of age and periodontal disease.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1237-1250"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of Lipophosphonoxin-loaded Polycaprolactone Nanofiber Dressing for Full-thickness Wounds in a Porcine Model.","authors":"Hubert Šuca, Štefan Juhás, Jana Juhásová, Věra Jenčová, Eva Kuželová Košťáková, David Lukáš, Peter Bohuš, Zuzana Ballová, Robert Zajíček, Dominik Rejman, Peter Gál","doi":"10.21873/invivo.13937","DOIUrl":"https://doi.org/10.21873/invivo.13937","url":null,"abstract":"<p><strong>Background/aim: </strong>This study investigated the therapeutic potential of lipophosphonoxin (LPPO), an antibacterial agent, loaded into polycaprolactone nanofiber dressings (NANO-LPPO) for full-thickness wound healing. Using a porcine model, we aimed to assess the impact of areal weight of the dressing (10, 20 and 30 g/m²) on wound-healing outcomes and validate findings from previous murine studies.</p><p><strong>Materials and methods: </strong>Full-thickness wounds were created on porcine skin and treated with the NANO-LPPO dressings of differing thickness. Positive control (Aquacel Ag+) and standard control (Jelonet) groups were included for comparison. Wound-healing progression was evaluated macroscopically and on the histological level.</p><p><strong>Results: </strong>Macroscopic observations indicated no signs of infection in any group, with wounds covered by scabs by day 14. Thicker dressings (areal weights of 30 and 20 g/m²) demonstrated superior performance in promoting the formation of granulation tissue and healing compared to the thinner version (areal weight of 10 g/m²). LPPO-loading enhanced scaffold wettability and biodegradability without impairing healing outcomes. Both control groups exhibited similar healing characteristics.</p><p><strong>Conclusion: </strong>The findings underscore the importance of optimizing dressing thickness for effective wound healing. NANO-LPPO dressings exhibit translational potential as a therapeutic option for full-thickness wounds, warranting further preclinical and regulatory evaluation to support clinical application.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1331-1340"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}