In vivo最新文献

{"title":"Validation of the Chick Chorioallantoic Membrane (CAM) Model for Biocompatibility Analysis of Biomaterials in the Context of the 3R-cascade: A Pilot Study.","authors":"Mike Barbeck, Kerstin Huebner, Kim Burkhardt, Tonya Andreeva, Rumen Krastev, Reiner Schnettler, Sanja Stojanovic, Stevo Najman, Ole Jung, Regine Schneider-Stock","doi":"10.21873/invivo.13977","DOIUrl":"https://doi.org/10.21873/invivo.13977","url":null,"abstract":"<p><strong>Background/aim: </strong>The present study aimed to compare the tissue responses to biomaterials in the chick chorioallantoic membrane (CAM) model with those from the subcutaneous implantation model in rats at an early time point. It was especially investigated whether histopathological scoring according to DIN EN ISO 10993-6 is also possible after biomaterial implantation using the CAM model and to what extent the values differ from the data obtained from small animal experiments.</p><p><strong>Materials and methods: </strong>Implantation of a xenogeneic bone substitute using the CAM model for 24 h and subcutaneous implantation model in rats up to 10 days post implantation were conducted. Standardized histological and histopathological methods were used to apply for histopathological scoring according to DIN EN ISO 10993-6.</p><p><strong>Results: </strong>The histological analysis as well as the histo-pathological scoring revealed that the tissue responses to the xenogeneic bone substitute were completely comparable in both organisms with no visible or statistical differences.</p><p><strong>Conclusion: </strong>We suggest that bioincompatible biomaterials can already be sorted out in the context of THE preclinical <i>in vivo</i> test phase. Such pre-testing before the required small animal tests might clearly contribute to the 3R-concept to reduce the number of animals (REDUCE).</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1740-1750"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041968/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spot Scanning Proton Beam Therapy for Surgery-inaccessible Hepatocellular Carcinoma: Preliminary Results.","authors":"Yutaka Fujimoto, Sachika Shiraishi, Masashi Yamanaka, Akihiro Yamano, Kazuki Matsumoto, Shintaro Shiba, Toshitaka Tsukiyama, Masahiro Kobayashi, Koichi Tokuuye","doi":"10.21873/invivo.13966","DOIUrl":"https://doi.org/10.21873/invivo.13966","url":null,"abstract":"<p><strong>Background/aim: </strong>Spot scanning proton beam therapy (SS-PBT) was employed for the treatment of surgery-inaccessible hepatocellular carcinoma, primarily influenced by respiratory movement.</p><p><strong>Patients and methods: </strong>Between October 2022 and December 2023, 12 patients were enrolled in this study to evaluate the efficacy of SS-PBT. The median follow-up time was 13.1 months.</p><p><strong>Results: </strong>The one-year survival, progression-free survival, and local control rates were 79.5%, 57.1%, and 100%, respectively, without grade 2 or higher PBT-related toxicities. The clinical outcomes of these 12 patients appear comparable to data from prospective studies conducted at proton centers across Japan.</p><p><strong>Conclusion: </strong>SS-PBT shows promise as a treatment option for surgery-inaccessible hepatocellular carcinoma.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1654-1660"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effectiveness of Live Birth Rate of Traditional Chinese Medicine Intervention for Infertile Women Undergoing a Second Round of IVF Is Influenced by Age.","authors":"Shu-Chiu Wang, Liang-Miin Tsai, Po-Ming Chen, Yuan-Man Hsu, Shu-Chen Wang, Chiung-Hung Chang","doi":"10.21873/invivo.13963","DOIUrl":"https://doi.org/10.21873/invivo.13963","url":null,"abstract":"<p><strong>Background/aim: </strong>While <i>in vitro</i> fertilization (IVF) is an effective treatment for infertility, the live birth rate remains suboptimal. This study aimed to evaluate the factors influencing live birth rates in infertile women undergoing a second round of IVF combined with traditional Chinese medicine (TCM) treatment.</p><p><strong>Patients and methods: </strong>This was a single-center, retrospective cohort study involving 45 infertile women undergoing a second round of IVF who underwent a combined treatment approach integrating TCM with IVF. Clinical factors affecting live birth rates were analyzed using Student's <i>t</i> test, Pearson's Chi-square test, and univariate logistic regression models.</p><p><strong>Results: </strong>The findings revealed a clinical pregnancy rate of 51.11% and a live birth rate of 42.22%, underscoring the potential effectiveness of combining TCM with a second round of IVF for infertile women. Additionally, maternal age showed a statistically significant influence on the clinical pregnancy rate (39.32±2.93 years <i>vs.</i> 36.48±2.47 years, <i>p</i>=0.005) and live birth rate (39.68±2.85 years <i>vs.</i> 35.58±2.95 years, <i>p</i><0.001).</p><p><strong>Conclusion: </strong>Integrating traditional Chinese medicine into IVF protocols notably enhances maternal success rates. For women over 38, age-specific strategies such as fertility preservation, optimized ovarian stimulation, advanced embryo selection, and oocyte donation are key to addressing age-related challenges.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1625-1637"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age- and Periodontal Disease Independent Correlation of Salivary Amino Acids.","authors":"Shoji Tanaka, Hiroshi Sakagami, Masahiro Sugimoto","doi":"10.21873/invivo.13928","DOIUrl":"https://doi.org/10.21873/invivo.13928","url":null,"abstract":"<p><strong>Background/aim: </strong>This study explored the relationship between salivary metabolomic profiles, periodontal diseases, and age.</p><p><strong>Patients and methods: </strong>Resting whole saliva samples were collected from a cohort comprising 21 women and 30 men aged 20 to 70 years, including healthy volunteers and patients with different stages of periodontal diseases. Hydrophilic metabolites were analyzed using capillary electrophoresis-mass spectrometry. The concentrations were quantified and analyzed using multivariable analysis with or without normalization to eliminate overall differences in salivary concentrations across the samples.</p><p><strong>Results: </strong>Metabolomic analysis quantified the absolute concentration of 248 metabolites in saliva samples. The unnormalized metabolomic profiles formed large clusters, with more than half of the detected metabolites showing positive correlations with each other. The absence of such clusters in the normalized data suggests the presence of individual differences in the processed data. The presence of urea, whose concentration increased gradually with the degree of progression of periodontal disease, and leucine, whose concentration decreased gradually, was identified. Highly positive correlations were observed between proline and glycine, which remained consistent regardless of normalization, age, or disease progression.</p><p><strong>Conclusion: </strong>The metabolomic profiles of salivary samples revealed unique correlations between amino acids that were independent of age and periodontal disease.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1237-1250"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In Vivo</i> Antitumor Activity of Allicin in a Pediatric Neuroblastoma Patient-derived Xenograft (PDX) Mouse Model.","authors":"Chad R Schultz, Martin C H Gruhlke, Alan J Slusarenko, André S Bachmann","doi":"10.21873/invivo.13932","DOIUrl":"https://doi.org/10.21873/invivo.13932","url":null,"abstract":"<p><strong>Background/aim: </strong>Allicin is a small-molecule natural product found in garlic (Allium sativum). We previously showed that allicin inhibits ornithine decarboxylase (ODC) <i>in vitro</i> and induces apoptotic cell death in pediatric neuroblastoma (NB) cancer cell cultures. However, its potency as an anticancer agent <i>in vivo</i> has not been sufficiently explored.</p><p><strong>Materials and methods: </strong>In this study, we used cell proliferation assays, immunoblotting techniques, and light microscopy to study NB tumor cell cultures and human primary neonatal skin fibroblast control cells as well as a <i>MYCN</i>-amplified NB patient-derived xenograft (PDX) mouse tumor model to study the efficacy of allicin <i>in vivo</i>.</p><p><strong>Results: </strong>Allicin strongly inhibits NB tumor cell proliferation in a dose-dependent manner while non-cancerous human primary neonatal skin fibroblast control cells were largely unaffected. Importantly, two intra-tumoral injections of allicin over a two-week trial period significantly reduced the NB tumor burden in mice compared to controls (N=4-9 mice/group). Excised tumor tissues revealed that allicin treatment increased the cyclin-dependent kinase inhibitor p27^Kip1 protein levels, suggesting that <i>in vivo</i>, allicin increases p27^Kip1-mediated G₁/S cell cycle arrest.</p><p><strong>Conclusion: </strong>Our findings warrant further preclinical development of allicin as a potential anticancer agent, especially for those types of cancers that are treatable by intra-tumoral injections, including neuroblastoma, glioblastoma, and medulloblastoma.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1283-1292"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041990/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship Between Frailty and Drug Burden Index in Older Hospitalized Patients.","authors":"Yukako Morisaki, Misuzu Takashima, Ayako Maeda-Minami, Sayaka Izumi, Masanori Suzuki, Ryohkan Funakoshi, Yasunari Mano","doi":"10.21873/invivo.13971","DOIUrl":"https://doi.org/10.21873/invivo.13971","url":null,"abstract":"<p><strong>Background/aim: </strong>In a super-aging society, understanding the prescription status of drug burden index (DBI) drugs that have anticholinergic and sedative effects in patients with frailty to consider proper medical intervention and promote appropriate drug use for older adults is important. This study evaluated the association between frailty and the use of DBI drugs in older hospitalized patients using hospital electronic medical records.</p><p><strong>Patients and methods: </strong>This cross-sectional study included patients admitted to the Kameda Medical Center between October 1, 2016 and October 31, 2017. Patients with a Barthel Index of <90 or Mini-Mental State Examination score of <18 or otherwise were classified into the frailty and non-frailty groups, respectively. DBI drugs fall into nine categories based on previous studies, and 162 drugs marketed in Japan were included. Patients using DBI drugs were considered DBI drug users; otherwise, patients were considered DBI drug non-users. Comparisons of the DBI drug proportions in both groups were performed using logistic regression analysis while adjusting for patient background factors and calculating the adjusted odds ratios and 95% confidence intervals.</p><p><strong>Results: </strong>The proportion of DBI drug users was significantly lower in the frailty group compared to the non-frailty group (adjusted odds ratio=0.32, 95% confidence interval=0.24-0.42, <i>p</i><0.001).</p><p><strong>Conclusion: </strong>Hospitalized older patients with frailty in Japan may be associated with a lower risk of DBI drug use and may use drugs with caution. In clinical practice, drug treatment for older patients may be implemented in consideration of various patient backgrounds, including frailty.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1694-1792"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adverse Reactions With VEGF Inhibitors in Combination With NSAIDs: Disproportionality Analysis Using JADRE and FAERS.","authors":"Kazuki Saito, Satoru Nihei, Junichi Asaka, Kenzo Kudo","doi":"10.21873/invivo.13947","DOIUrl":"https://doi.org/10.21873/invivo.13947","url":null,"abstract":"<p><strong>Background/aim: </strong>The concurrent use of vascular endothelial growth factor (VEGF) inhibitors and nonsteroidal anti-inflammatory drugs (NSAIDs) raises concerns regarding the increased risk of adverse drug reactions (ADRs) due to potential pharmacodynamic interactions. However, no studies have specifically addressed this issue. The objective of this study was to investigate whether the combination of these drugs increased the risk of ADRs.</p><p><strong>Patients and methods: </strong>Disproportionality analysis was conducted on ADR reports from the Japanese Adverse Drug Event Report (JADER) and FDA Adverse Event Reporting System (FAERS) databases. The concomitant signal score and Ω shrinkage measure were used to identify safety signals associated with the drug combination. Additionally, logistic regression analysis focused on reports of ADRs related to cancer treatment and assessed the significance of the adjusted reporting odds ratio (aROR) for the interaction between these drugs.</p><p><strong>Results: </strong>Disproportionality analysis included ADR data from the JADER (<i>n</i>=1,509,399) and FAERS (<i>n</i>=38,610,433) databases. The concomitant signal score and Ω shrinkage measure identified a signal for gastrointestinal perforation in both databases. Logistic regression on cancer treatment-related ADRs (JADER: <i>n</i>=255,177; FAERS: <i>n</i>=1,167,941) showed a synergistic increase in gastrointestinal perforation risk with the drug combination [aROR for interaction term: JADER: 1.74 (95% confidence interval (CI)=1.45-2.07); FAERS: 1.49 (95% CI=1.29-1.72)].</p><p><strong>Conclusion: </strong>The combination of VEGF inhibitors and NSAIDs is associated with an increased risk of gastrointestinal perforation, a serious and potentially fatal ADR. Therefore, caution is warranted when prescribing a combination of these drugs.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1458-1469"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041964/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bilateral Venous Access for Cardiac Resynchronization Therapy in a Hemodialysis Patient With Cabozantinib-associated Heart Failure.","authors":"Amane Otoi, Akinori Higaki, Noriyoshi Miura, Keisho Kurokawa, Kohei Yoshimoto, Tomoaki Nishikawa, Rikako Horie, Arisa Abe, Yasuhisa Nakao, Tomoki Fujisawa, Shigehiro Miyazaki, Yusuke Akazawa, Toru Miyoshi, Hiroshi Kawakami, Haruhiko Higashi, Shunsuke Tamaki, Kazuhisa Nishimura, Katsuji Inoue, Shuntaro Ikeda, Osamu Yamaguchi","doi":"10.21873/invivo.13973","DOIUrl":"https://doi.org/10.21873/invivo.13973","url":null,"abstract":"<p><strong>Background: </strong>Cabozantinib, a multi-targeted tyrosine kinase inhibitor, is widely used for the treatment of renal and hepatic cancers. While cabozantinib-associated cardiotoxicity is rare, it has been documented in several cases. In most instances, cancer therapeutics-related cardiac dysfunction (CTRCD) is managed by discontinuing cabozantinib and initiating cardioprotective agents. In this report, we present the case of a 63-year-old male with cabozantinib-induced heart failure (HF) with reduced ejection fraction (EF) and complete left bundle branch block (CLBBB).</p><p><strong>Case report: </strong>The patient, undergoing hemodialysis for chronic kidney disease, had limited therapeutic options due to prior treatment failures. Despite six months of standard HF therapy, symptoms persisted, prompting cardiac resynchronization therapy (CRT) implantation without interrupting cabozantinib. Due to the presence of a dialysis shunt in the patient's left arm, the right subclavian vein was selected for venous access to minimize the risk of lead-related complications. Using a tunneling tool, the left ventricular lead was placed <i>via</i> the contralateral vasculature to the ipsilateral generator. Six months post-CRT, echocardiography showed significant reverse remodeling with improved EF and reduced left ventricular end-diastolic diameter, alongside clinical symptom relief.</p><p><strong>Conclusion: </strong>This case highlights the utility of bilateral venous access with a tunneling tool in cardiac resynchronization therapy, particularly for patients with hemodialysis shunts.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1719-1723"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041996/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neutrophil-to-Lymphocyte Ratio as a Biomarker for Postoperative Complications in Crohn's Disease.","authors":"Makoto Kawamoto, Daijiro Higashi, Ken Kinjo, Noritaka Takatsu, Yoshihiro Miyasaka, Hisatomi Arima, Satoshi Nimura, Takashi Hisabe, Masato Watanabe","doi":"10.21873/invivo.13958","DOIUrl":"https://doi.org/10.21873/invivo.13958","url":null,"abstract":"<p><strong>Background/aim: </strong>Despite advances in diagnosis and pharmacotherapy, surgery remains crucial for Crohn's disease (CD). Postoperative intra-abdominal septic complications (IASC) occur in 1.2-16.7% of cases. We investigated the frequency of postoperative IASC in elective surgeries for CD and the risk factors and potential biomarkers for postoperative IASC.</p><p><strong>Patients and methods: </strong>We conducted a retrospective single-center cohort study of patients who underwent abdominal surgery for CD at Fukuoka University Chikushi Hospital between January 2015 and December 2023. The primary focus was the incidence of IASC within 60 days postoperatively. Patient-related variables were examined using univariate and multivariable analyses.</p><p><strong>Results: </strong>The analysis included 206 of 249 surgeries. Postoperative IASC occurred in 26 patients (12.6%). Univariate analysis identified history of steroid use requiring steroid coverage (<i>p</i>=0.002), penetrating type (<i>p</i>=0.020), WBC count (<i>p</i>=0.037), neutrophil count (0.009), C-reactive protein (CRP) (<i>p</i>=0.035), CRP-albumin ratio (CAR) (<i>p</i>=0.034), neutrophil-to-lymphocyte ratio (NLR) (<i>p</i>=0.002), and operation duration (<i>p</i>=0.010) as significant factors. Multivariable analysis identified history of steroid use requiring steroid coverage (OR=6.23, 95%CI=1.61-24.1, <i>p</i>=0.008), high NLR (OR=3.43, 95%CI=1.30-9.04, <i>p</i>=0.013), and long duration of operation (OR=2.63, 95%CI=1.01-6.88, <i>p</i>=0.049) as independent predictors. The optimal cutoffs for predicting IASC were an NLR of 3.98 (sensitivity, 61.5%; specificity, 77.8%) and an operation time of 173 min (sensitivity, 65.4%; specificity, 65.0%), respectively.</p><p><strong>Conclusion: </strong>History of steroid use requiring steroid coverage, preoperative NLR ≧3.98, and duration of operation ≧173 min are independent risk factors for postoperative IASC in elective surgeries for CD. Recognition of high-risk patients would contribute to the decision-making process for perioperative management.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1580-1590"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Feasibility of Common Enterotomy Closure Using Bidirectional Barbed Sutures in Intracorporeal Overlap Anastomosis During Robotic Surgery for Colon Cancer.","authors":"Masatsune Shibutani, Tatsunari Fukuoka, Hiroaki Kasashima, Shintaro Ozawa, Hideki Tanda, Ken Yonemitsu, Yuki Seki, Kiyoshi Maeda","doi":"10.21873/invivo.13956","DOIUrl":"https://doi.org/10.21873/invivo.13956","url":null,"abstract":"<p><strong>Background/aim: </strong>Although intracorporeal anastomosis in minimally invasive colectomy has many advantages, it requires a longer operative time than extracorporeal anastomosis. For quick and reliable common enterotomy closure, we proposed a new method using bidirectional barbed sutures. The present study evaluated the safety and feasibility of common enterotomy closure using bidirectional barbed sutures in intracorporeal overlap anastomosis during robotic surgery for colon cancer.</p><p><strong>Patients and methods: </strong>A total of 39 patients who underwent common enterotomy closure using bidirectional barbed sutures in intracorporeal overlap anastomosis during robotic surgery for colon cancer were enrolled in this study.</p><p><strong>Results: </strong>Although minor infectious complications were observed in a few cases, no anastomotic leakage or stricture was observed.</p><p><strong>Conclusion: </strong>Common enterotomy closure <i>via</i> a new method using bidirectional barbed sutures in intracorporeal overlap anastomosis may be a safe and useful procedure, especially in hospitals newly introducing intracorporeal anastomosis.</p>","PeriodicalId":13364,"journal":{"name":"In vivo","volume":"39 3","pages":"1567-1572"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}